Title: APC mutation correlated with poor response of immunotherapy in colon cancer
Version: 2 Date: 18 Jan 2023
Reviewer's report:
Overall, this is an interesting study. Using a computational approach, the authors show that in
colon cancer patients, the presence of adenomatous polyposis coli (APC) mutation correlated
with a less effective immunotherapy outcome. In my opinion, the manuscript is suitable for
publication after the authors have addressed the following questions and comments
1. In Table 1, the authors suggest that there is no correlation between APC mutation and tumor
mutational burden (TMB) while comparing APC mutant and wildtype group in the MSKCC
dataset. However, in Figure 4, the authors show that APC mutation is associated with lower
TMB. How can the authors explain this apparent contradiction in their findings?
2. The authors present interesting findings in Figure 5. However, it would be nice to see if the
computational findings correlate with experimental data. Could the authors stain patient samples
(APC wildtype and mutants) to look at immune cell infiltration?
3. The manuscript is well organized, and it is easy to follow the logical flow of ideas and
the rationale of the study. However, there are several grammatical and typographical errors
throughout the manuscript.  The manuscript needs to be carefully revised to improve the written
content.
Title: APC mutation correlated with poor response of immunotherapy in colon cancer
Version: 2 Date: 18 Jan 2023
Reviewer's report:
Overall, this is an interesting study. Using a computational approach, the authors show that in
colon cancer patients, the presence of adenomatous polyposis coli (APC) mutation correlated
with a less effective immunotherapy outcome. In my opinion, the manuscript is suitable for
publication after the authors have addressed the following questions and comments
1. In Table 1, the authors suggest that there is no correlation between APC mutation and tumor
mutational burden (TMB) while comparing APC mutant and wildtype group in the MSKCC
dataset. However, in Figure 4, the authors show that APC mutation is associated with lower
TMB. How can the authors explain this apparent contradiction in their findings?
2. The authors present interesting findings in Figure 5. However, it would be nice to see if the
computational findings correlate with experimental data. Could the authors stain patient samples
(APC wildtype and mutants) to look at immune cell infiltration?
3. The manuscript is well organized, and it is easy to follow the logical flow of ideas and
the rationale of the study. However, there are several grammatical and typographical errors
throughout the manuscript.  The manuscript needs to be carefully revised to improve the written
content.
Please review this paper.
user
Title: APC mutation correlated with poor response of immunotherapy in colon cancer
Version: 2 Date: 18 Jan 2023
Reviewer's report:
Overall, this is an interesting study. Using a computational approach, the authors show that in
colon cancer patients, the presence of adenomatous polyposis coli (APC) mutation correlated
with a less effective immunotherapy outcome. In my opinion, the manuscript is suitable for
publication after the authors have addressed the following questions and comments
1. In Table 1, the authors suggest that there is no correlation between APC mutation and tumor
mutational burden (TMB) while comparing APC mutant and wildtype group in the MSKCC
dataset. However, in Figure 4, the authors show that APC mutation is associated with lower
TMB. How can the authors explain this apparent contradiction in their findings?
2. The authors present interesting findings in Figure 5. However, it would be nice to see if the
computational findings correlate with experimental data. Could the authors stain patient samples
(APC wildtype and mutants) to look at immune cell infiltration?
3. The manuscript is well organized, and it is easy to follow the logical flow of ideas and
the rationale of the study. However, there are several grammatical and typographical errors
throughout the manuscript.  The manuscript needs to be carefully revised to improve the written
content.
assistant