Title: Host cytokine genetic polymorphisms in a selected population of persons living with
hepatitis B virus infection in the Central Region of Ghana
Version: 2 Date: 23 Jul 2024
Reviewer:  Kingsley Andrew Egbe
Reviewer's report:
The article presents intriguing insights that will significantly contribute to our understanding of
“Host cytokine genetic polymorphisms in a selected population of persons living with hepatitis
B virus infection in the Central Region of Ghana.”
However, several observations have been noted. The authors should review the manuscript for
grammatical accuracy and correct the identified errors, such as:
Abstract.
Under the background section of the abstract, the 4th line reads,  The outcome of HBV infection
is influenced by viral and hots factors including genetic. It should be viral host factors, including
genetics.
Under the result section of the abstract, the starting: There 20–39-year-old group formed
majority of the HBV infected  participants. The authors should consider rewording the sentence
accordingly for grammatical accuracy.
Methods
The authors should indicate when the sampling was done
Ethical consideration
The authors should consider checking for grammatical accuracy.
After the reference number it should read…..Participants who met the inclusion criteria were
approached at the clinic during their visit and informed of the study, and those who were willing
were enrolled.
Laboratory management
The authors should include the instrument for blood collection and how the plasma was
processed as indicated in the manuscript.
HBV antigen profile test
The authors should confirm whether it is Wondo or Wondfo and maintain consistency.
DNA Extraction and cytokine genotyping
The reverse primer sequence for TNF-# should be in capital letters.
Statistical Analysis
The authors should consider including the type of data collected using KoBo.
Results
Table 2
The explanation for table 2 should be checked for grammatical accuracy.
Figure 3:
The pair-wise LD association between three SNPs and the corresponding D’ and R’ values. The
colour gradient from red to white reveals higher?
Discussion
Line 16 reads: In a study that looked inactive HBV inactive carriers, it was discovered that 92%
of patients were inactive carriers which was much higher than our cases, although they have a
reduced sample size of 52 (38). It should be properly stated.
The authors should consider including and stressing the relevance of the study to global
discourse. For example, what specific target (not just to mention Goal 1, Goal 2, etc.)  in the
SDGs or AU Agenda 2063 does the findings of this study contribute to?
Title: Host cytokine genetic polymorphisms in a selected population of persons living with
hepatitis B virus infection in the Central Region of Ghana
Version: 2 Date: 23 Jul 2024
Reviewer:  Kingsley Andrew Egbe
Reviewer's report:
The article presents intriguing insights that will significantly contribute to our understanding of
“Host cytokine genetic polymorphisms in a selected population of persons living with hepatitis
B virus infection in the Central Region of Ghana.”
However, several observations have been noted. The authors should review the manuscript for
grammatical accuracy and correct the identified errors, such as:
Abstract.
Under the background section of the abstract, the 4th line reads,  The outcome of HBV infection
is influenced by viral and hots factors including genetic. It should be viral host factors, including
genetics.
Under the result section of the abstract, the starting: There 20–39-year-old group formed
majority of the HBV infected  participants. The authors should consider rewording the sentence
accordingly for grammatical accuracy.
Methods
The authors should indicate when the sampling was done
Ethical consideration
The authors should consider checking for grammatical accuracy.
After the reference number it should read…..Participants who met the inclusion criteria were
approached at the clinic during their visit and informed of the study, and those who were willing
were enrolled.
Laboratory management
The authors should include the instrument for blood collection and how the plasma was
processed as indicated in the manuscript.
HBV antigen profile test
The authors should confirm whether it is Wondo or Wondfo and maintain consistency.
DNA Extraction and cytokine genotyping
The reverse primer sequence for TNF-# should be in capital letters.
Statistical Analysis
The authors should consider including the type of data collected using KoBo.
Results
Table 2
The explanation for table 2 should be checked for grammatical accuracy.
Figure 3:
The pair-wise LD association between three SNPs and the corresponding D’ and R’ values. The
colour gradient from red to white reveals higher?
Discussion
Line 16 reads: In a study that looked inactive HBV inactive carriers, it was discovered that 92%
of patients were inactive carriers which was much higher than our cases, although they have a
reduced sample size of 52 (38). It should be properly stated.
The authors should consider including and stressing the relevance of the study to global
discourse. For example, what specific target (not just to mention Goal 1, Goal 2, etc.)  in the
SDGs or AU Agenda 2063 does the findings of this study contribute to?
Please review this paper.
user
Title: Host cytokine genetic polymorphisms in a selected population of persons living with
hepatitis B virus infection in the Central Region of Ghana
Version: 2 Date: 23 Jul 2024
Reviewer:  Kingsley Andrew Egbe
Reviewer's report:
The article presents intriguing insights that will significantly contribute to our understanding of
“Host cytokine genetic polymorphisms in a selected population of persons living with hepatitis
B virus infection in the Central Region of Ghana.”
However, several observations have been noted. The authors should review the manuscript for
grammatical accuracy and correct the identified errors, such as:
Abstract.
Under the background section of the abstract, the 4th line reads,  The outcome of HBV infection
is influenced by viral and hots factors including genetic. It should be viral host factors, including
genetics.
Under the result section of the abstract, the starting: There 20–39-year-old group formed
majority of the HBV infected  participants. The authors should consider rewording the sentence
accordingly for grammatical accuracy.
Methods
The authors should indicate when the sampling was done
Ethical consideration
The authors should consider checking for grammatical accuracy.
After the reference number it should read…..Participants who met the inclusion criteria were
approached at the clinic during their visit and informed of the study, and those who were willing
were enrolled.
Laboratory management
The authors should include the instrument for blood collection and how the plasma was
processed as indicated in the manuscript.
HBV antigen profile test
The authors should confirm whether it is Wondo or Wondfo and maintain consistency.
DNA Extraction and cytokine genotyping
The reverse primer sequence for TNF-# should be in capital letters.
Statistical Analysis
The authors should consider including the type of data collected using KoBo.
Results
Table 2
The explanation for table 2 should be checked for grammatical accuracy.
Figure 3:
The pair-wise LD association between three SNPs and the corresponding D’ and R’ values. The
colour gradient from red to white reveals higher?
Discussion
Line 16 reads: In a study that looked inactive HBV inactive carriers, it was discovered that 92%
of patients were inactive carriers which was much higher than our cases, although they have a
reduced sample size of 52 (38). It should be properly stated.
The authors should consider including and stressing the relevance of the study to global
discourse. For example, what specific target (not just to mention Goal 1, Goal 2, etc.)  in the
SDGs or AU Agenda 2063 does the findings of this study contribute to?
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