Title: Correlation and Diagnostic Significance of CD4 T Cell Subsets and NLRP3
Inflammasome in Ulcerative Colitis: The Role of the NLRP3/T-bet/GATA3 Axis
Version: 1 Date: 18 Nov 2024
Reviewer:  Aleksandra Piechota-Polanczyk
Reviewer's report:
The article entitled „Correlation and Diagnostic Significance of CD4 T Cell Subsets and
NLRP3 Inflammasome in Ulcerative Colitis: The Role of the NLRP3/T-bet/GATA3 Axis”  is
an interesting original paper on Chinees population with UC. The authors analyse relatively
big cohorts with two stages of UC and control patients. They measure several markers of
inflammation using different immunoenzymatic methods. The results are clearly presented and
discussed but could be improved. My detailed comments are below.
Methods:
IHC- the authors should specify how many areas per patient were analyse, and how the positive
signal was analysed.
The authors should add drug history of analysed patients and discuss if taken medications should
influence T cells cohorts.
Results:
Figures with IHC stainings- the authors should reduce the pinkish background which shades
DAB staining and strongly interrupts with data interpretation.
Figure 2- IFN-gamma- there are two outliers- did the authors use Grubb’s test to exclude
significant outliers. It looks like this could be significant outliers.
Figure 4- there is no description of panel B
The authors could analyse the populations of T cells in the blood and add this information to
correlation studies.
The authors should clarify what they mean by “UC-LM”, “UC-S”, “UC-L”, and “UC-MH” on
each figure.
Results and discussion:
The authors have equal number of males and females- they should consider doing additional
analysis in sex-divided subgroups and fluctuating hormonal levels in females could influence
inflammatory reaction within the colon tissue.
There is no rationale behind panel Fig 4B. How the authors determined binding affinity? It is
not visible in a created panel.
The authors conclude that “By suppressing T-bet, IL-6 hinders Th1 responses and upregulates
GATA3, suggesting that targeting this axis could effectively manage UC by addressing both
inflammation and regulation”. Does it have clinical value? Can the authors suggest any drug
which could target this axis?
Title: Correlation and Diagnostic Significance of CD4 T Cell Subsets and NLRP3
Inflammasome in Ulcerative Colitis: The Role of the NLRP3/T-bet/GATA3 Axis
Version: 1 Date: 18 Nov 2024
Reviewer:  Aleksandra Piechota-Polanczyk
Reviewer's report:
The article entitled „Correlation and Diagnostic Significance of CD4 T Cell Subsets and
NLRP3 Inflammasome in Ulcerative Colitis: The Role of the NLRP3/T-bet/GATA3 Axis”  is
an interesting original paper on Chinees population with UC. The authors analyse relatively
big cohorts with two stages of UC and control patients. They measure several markers of
inflammation using different immunoenzymatic methods. The results are clearly presented and
discussed but could be improved. My detailed comments are below.
Methods:
IHC- the authors should specify how many areas per patient were analyse, and how the positive
signal was analysed.
The authors should add drug history of analysed patients and discuss if taken medications should
influence T cells cohorts.
Results:
Figures with IHC stainings- the authors should reduce the pinkish background which shades
DAB staining and strongly interrupts with data interpretation.
Figure 2- IFN-gamma- there are two outliers- did the authors use Grubb’s test to exclude
significant outliers. It looks like this could be significant outliers.
Figure 4- there is no description of panel B
The authors could analyse the populations of T cells in the blood and add this information to
correlation studies.
The authors should clarify what they mean by “UC-LM”, “UC-S”, “UC-L”, and “UC-MH” on
each figure.
Results and discussion:
The authors have equal number of males and females- they should consider doing additional
analysis in sex-divided subgroups and fluctuating hormonal levels in females could influence
inflammatory reaction within the colon tissue.
There is no rationale behind panel Fig 4B. How the authors determined binding affinity? It is
not visible in a created panel.
The authors conclude that “By suppressing T-bet, IL-6 hinders Th1 responses and upregulates
GATA3, suggesting that targeting this axis could effectively manage UC by addressing both
inflammation and regulation”. Does it have clinical value? Can the authors suggest any drug
which could target this axis?
Please review this paper.
user
Title: Correlation and Diagnostic Significance of CD4 T Cell Subsets and NLRP3
Inflammasome in Ulcerative Colitis: The Role of the NLRP3/T-bet/GATA3 Axis
Version: 1 Date: 18 Nov 2024
Reviewer:  Aleksandra Piechota-Polanczyk
Reviewer's report:
The article entitled „Correlation and Diagnostic Significance of CD4 T Cell Subsets and
NLRP3 Inflammasome in Ulcerative Colitis: The Role of the NLRP3/T-bet/GATA3 Axis”  is
an interesting original paper on Chinees population with UC. The authors analyse relatively
big cohorts with two stages of UC and control patients. They measure several markers of
inflammation using different immunoenzymatic methods. The results are clearly presented and
discussed but could be improved. My detailed comments are below.
Methods:
IHC- the authors should specify how many areas per patient were analyse, and how the positive
signal was analysed.
The authors should add drug history of analysed patients and discuss if taken medications should
influence T cells cohorts.
Results:
Figures with IHC stainings- the authors should reduce the pinkish background which shades
DAB staining and strongly interrupts with data interpretation.
Figure 2- IFN-gamma- there are two outliers- did the authors use Grubb’s test to exclude
significant outliers. It looks like this could be significant outliers.
Figure 4- there is no description of panel B
The authors could analyse the populations of T cells in the blood and add this information to
correlation studies.
The authors should clarify what they mean by “UC-LM”, “UC-S”, “UC-L”, and “UC-MH” on
each figure.
Results and discussion:
The authors have equal number of males and females- they should consider doing additional
analysis in sex-divided subgroups and fluctuating hormonal levels in females could influence
inflammatory reaction within the colon tissue.
There is no rationale behind panel Fig 4B. How the authors determined binding affinity? It is
not visible in a created panel.
The authors conclude that “By suppressing T-bet, IL-6 hinders Th1 responses and upregulates
GATA3, suggesting that targeting this axis could effectively manage UC by addressing both
inflammation and regulation”. Does it have clinical value? Can the authors suggest any drug
which could target this axis?
assistant