Title: Differential expression of PLAC1 and Netrin-1 in liver metastasis of colorectal cancer
and its predictive value
Version: 1 Date: 25 Jun 2023
Reviewer's report:
1.Introduction section is too simple in describing the role of PLAC1 and Netrin-1 in diseases.
The author need to add relevant content of PLAC1 and Netrin-1 as predictive indicators in liver
metastasis in colorectal cancer, or other diseases.
2.Revise the descriptions: “ The content of PLAC1 in the tissues of CRC with liver metastasis
had a close relationship with differentiation degree and lymph node metastasis (P<0.05). The
expression of Netrin-1 in the tissues of CRC with liver metastasis was associated with Dukes
stage, differentiation degree and lymph node metastasis (P<0.05, Table 2)”. The expression is
inaccurate. Table 2 only compared different levels without conducting correlation analysis.
3. According to Table 4, the regression coefficients and constant terms are used to construct the
model, and the prediction model equation is Logit (P). Does this equation include PLAC1 and
Netrin-1 indicators, and does it deviate from the theme and content of the article?
4. "Logistic regression analysis showed that Dukes stage, differentiation degree, lymph node
metastasis, CEA, Alb and D-dimer were the independent risk factors for liver metastasis of CRC
(P<0.05, Table 4)." The statement is incorrect. All indicators should be assigned values before
multiple factor analysis. It is suggested to carry out two classifications. The risk factors should
reach a certain level
5. The discussion and analysis are lacking details. It is recommended to conduct a detailed
analysis based on the order of the tables, with a focus on analyzing the results with statistical
difference.
6.The two groups are divided according to whether liver metastasis occurs. The authors need to
state how to separate the groups first, followed by other data detection methods.
7. Add the sources, and origins for instruments, drugs, and reagents mentioned in the paper.
8. Add discussions about why PLAC1 and Netrin-1 can serve as factors affecting liver metastasis
in colorectal cancer and why they have high diagnostic value.
Title: Differential expression of PLAC1 and Netrin-1 in liver metastasis of colorectal cancer
and its predictive value
Version: 1 Date: 25 Jun 2023
Reviewer's report:
1.Introduction section is too simple in describing the role of PLAC1 and Netrin-1 in diseases.
The author need to add relevant content of PLAC1 and Netrin-1 as predictive indicators in liver
metastasis in colorectal cancer, or other diseases.
2.Revise the descriptions: “ The content of PLAC1 in the tissues of CRC with liver metastasis
had a close relationship with differentiation degree and lymph node metastasis (P<0.05). The
expression of Netrin-1 in the tissues of CRC with liver metastasis was associated with Dukes
stage, differentiation degree and lymph node metastasis (P<0.05, Table 2)”. The expression is
inaccurate. Table 2 only compared different levels without conducting correlation analysis.
3. According to Table 4, the regression coefficients and constant terms are used to construct the
model, and the prediction model equation is Logit (P). Does this equation include PLAC1 and
Netrin-1 indicators, and does it deviate from the theme and content of the article?
4. "Logistic regression analysis showed that Dukes stage, differentiation degree, lymph node
metastasis, CEA, Alb and D-dimer were the independent risk factors for liver metastasis of CRC
(P<0.05, Table 4)." The statement is incorrect. All indicators should be assigned values before
multiple factor analysis. It is suggested to carry out two classifications. The risk factors should
reach a certain level
5. The discussion and analysis are lacking details. It is recommended to conduct a detailed
analysis based on the order of the tables, with a focus on analyzing the results with statistical
difference.
6.The two groups are divided according to whether liver metastasis occurs. The authors need to
state how to separate the groups first, followed by other data detection methods.
7. Add the sources, and origins for instruments, drugs, and reagents mentioned in the paper.
8. Add discussions about why PLAC1 and Netrin-1 can serve as factors affecting liver metastasis
in colorectal cancer and why they have high diagnostic value.
Please review this paper.
user
Title: Differential expression of PLAC1 and Netrin-1 in liver metastasis of colorectal cancer
and its predictive value
Version: 1 Date: 25 Jun 2023
Reviewer's report:
1.Introduction section is too simple in describing the role of PLAC1 and Netrin-1 in diseases.
The author need to add relevant content of PLAC1 and Netrin-1 as predictive indicators in liver
metastasis in colorectal cancer, or other diseases.
2.Revise the descriptions: “ The content of PLAC1 in the tissues of CRC with liver metastasis
had a close relationship with differentiation degree and lymph node metastasis (P<0.05). The
expression of Netrin-1 in the tissues of CRC with liver metastasis was associated with Dukes
stage, differentiation degree and lymph node metastasis (P<0.05, Table 2)”. The expression is
inaccurate. Table 2 only compared different levels without conducting correlation analysis.
3. According to Table 4, the regression coefficients and constant terms are used to construct the
model, and the prediction model equation is Logit (P). Does this equation include PLAC1 and
Netrin-1 indicators, and does it deviate from the theme and content of the article?
4. "Logistic regression analysis showed that Dukes stage, differentiation degree, lymph node
metastasis, CEA, Alb and D-dimer were the independent risk factors for liver metastasis of CRC
(P<0.05, Table 4)." The statement is incorrect. All indicators should be assigned values before
multiple factor analysis. It is suggested to carry out two classifications. The risk factors should
reach a certain level
5. The discussion and analysis are lacking details. It is recommended to conduct a detailed
analysis based on the order of the tables, with a focus on analyzing the results with statistical
difference.
6.The two groups are divided according to whether liver metastasis occurs. The authors need to
state how to separate the groups first, followed by other data detection methods.
7. Add the sources, and origins for instruments, drugs, and reagents mentioned in the paper.
8. Add discussions about why PLAC1 and Netrin-1 can serve as factors affecting liver metastasis
in colorectal cancer and why they have high diagnostic value.
assistant