Reviewer's report
Title:Paraoxonase-1 is related to inflammation, fibrosis and PPAR delta in
experimental liver disease
Version: 1Date:27 September 2008
Reviewer: Nathalie Janel
Reviewer's report:
Marsillach and colleagues showed the relationships between paraoxonase-1
(PON1) expression and MCP-1 production, in a experimental model of chronic
liver disease. This is an interesting study, but three points need to be clarified in
order to accept the figure 6.
Major Compulsory Revisions:
In figure 2, despite the increased hepatic PON1 protein expression, the serum
PON1 expression was decreased. Can you explain this important point?
In figure 4, there is an increased activity of cathepsin B at 6 weeks of CCL4
treatment compared to control, but an increased hepatic PON1 concentration.
Can you really assume that the decreased PON1 protein degradation explains
the increase hepatic levels of PON1 at 6 weeks of treatment?
In figure 5, despite the increased hepatic PON1 protein expression, the lactonase
activity was decreased. Can you explain this important point?
Minor Essential Revisions:
In figure 1, if you give histograms for control and treated rats, we should added
the micrographs of protein expression for the control group.
Level of interest: An article of outstanding merit and interest in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests below.
Reviewer's report
Title:Paraoxonase-1 is related to inflammation, fibrosis and PPAR delta in
experimental liver disease
Version: 1Date:27 September 2008
Reviewer: Nathalie Janel
Reviewer's report:
Marsillach and colleagues showed the relationships between paraoxonase-1
(PON1) expression and MCP-1 production, in a experimental model of chronic
liver disease. This is an interesting study, but three points need to be clarified in
order to accept the figure 6.
Major Compulsory Revisions:
In figure 2, despite the increased hepatic PON1 protein expression, the serum
PON1 expression was decreased. Can you explain this important point?
In figure 4, there is an increased activity of cathepsin B at 6 weeks of CCL4
treatment compared to control, but an increased hepatic PON1 concentration.
Can you really assume that the decreased PON1 protein degradation explains
the increase hepatic levels of PON1 at 6 weeks of treatment?
In figure 5, despite the increased hepatic PON1 protein expression, the lactonase
activity was decreased. Can you explain this important point?
Minor Essential Revisions:
In figure 1, if you give histograms for control and treated rats, we should added
the micrographs of protein expression for the control group.
Level of interest: An article of outstanding merit and interest in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests below.
Please review this paper.
user
Reviewer's report
Title:Paraoxonase-1 is related to inflammation, fibrosis and PPAR delta in
experimental liver disease
Version: 1Date:27 September 2008
Reviewer: Nathalie Janel
Reviewer's report:
Marsillach and colleagues showed the relationships between paraoxonase-1
(PON1) expression and MCP-1 production, in a experimental model of chronic
liver disease. This is an interesting study, but three points need to be clarified in
order to accept the figure 6.
Major Compulsory Revisions:
In figure 2, despite the increased hepatic PON1 protein expression, the serum
PON1 expression was decreased. Can you explain this important point?
In figure 4, there is an increased activity of cathepsin B at 6 weeks of CCL4
treatment compared to control, but an increased hepatic PON1 concentration.
Can you really assume that the decreased PON1 protein degradation explains
the increase hepatic levels of PON1 at 6 weeks of treatment?
In figure 5, despite the increased hepatic PON1 protein expression, the lactonase
activity was decreased. Can you explain this important point?
Minor Essential Revisions:
In figure 1, if you give histograms for control and treated rats, we should added
the micrographs of protein expression for the control group.
Level of interest: An article of outstanding merit and interest in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests below.
assistant