Reviewer's report
Title:Epigenetic mechanisms involved in differential MDR1 mRNA expression
between gastric and colon cancer cell lines and rationales for clinical
chemotherapy
Version: 4Date:23 May 2008
Reviewer: Masakazu Yashiro
Reviewer's report:
Discretionary Revisions
The authors added new experimental data in response to the reviewers’
comments. They provided evidence of DNA methylation status by bisulfate DNA
sequencing assay. Authors demonstrated that methylation status by
quantification PCR-based methylation analysis were completely matched with the
results obtained by bisulfite DNA sequencing assay. They suggested that
complete methylation in the extended MS1 site was responsible for increased
MDR1 mRNA expression by the treatment with 5AC. In addition, MS1 site
derived from exon1 of MDR1 promoter has shown to be more important with
respect to gene expression than MS2 site from intron 1 of MDR1 promoter. This
DNA sequencing studies revised the manuscript extensively.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Reviewer's report
Title:Epigenetic mechanisms involved in differential MDR1 mRNA expression
between gastric and colon cancer cell lines and rationales for clinical
chemotherapy
Version: 4Date:23 May 2008
Reviewer: Masakazu Yashiro
Reviewer's report:
Discretionary Revisions
The authors added new experimental data in response to the reviewers’
comments. They provided evidence of DNA methylation status by bisulfate DNA
sequencing assay. Authors demonstrated that methylation status by
quantification PCR-based methylation analysis were completely matched with the
results obtained by bisulfite DNA sequencing assay. They suggested that
complete methylation in the extended MS1 site was responsible for increased
MDR1 mRNA expression by the treatment with 5AC. In addition, MS1 site
derived from exon1 of MDR1 promoter has shown to be more important with
respect to gene expression than MS2 site from intron 1 of MDR1 promoter. This
DNA sequencing studies revised the manuscript extensively.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Please review this paper.
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Reviewer's report
Title:Epigenetic mechanisms involved in differential MDR1 mRNA expression
between gastric and colon cancer cell lines and rationales for clinical
chemotherapy
Version: 4Date:23 May 2008
Reviewer: Masakazu Yashiro
Reviewer's report:
Discretionary Revisions
The authors added new experimental data in response to the reviewers’
comments. They provided evidence of DNA methylation status by bisulfate DNA
sequencing assay. Authors demonstrated that methylation status by
quantification PCR-based methylation analysis were completely matched with the
results obtained by bisulfite DNA sequencing assay. They suggested that
complete methylation in the extended MS1 site was responsible for increased
MDR1 mRNA expression by the treatment with 5AC. In addition, MS1 site
derived from exon1 of MDR1 promoter has shown to be more important with
respect to gene expression than MS2 site from intron 1 of MDR1 promoter. This
DNA sequencing studies revised the manuscript extensively.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
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