Title: Correlation between PDGF-BB and M1-type macrophage in inflammatory bowel disease:
a case-control study
Version: 1 Date: 30 Sep 2024
Reviewer:  Panagiotis Tsibouris
Reviewer's report:
Abstract results reporting should define serum and colonic measurements  They should report
the levels of PDGF in all patients and not in a small subgroup Methods Why authors selected
a 12 week instead of 24 or 48 week time to perform colonoscopy as most guidelines suggest
in order to verify remission Were there any inclusion or exclusion criteria apart from the
underlined disease, such as age, presence of comorbidities, presence of neoplasia, pregnancy
and lactation? Authors report that they sample the same area after entering remission How thay
have accomplish this task because any marking might have influenced sampling? Definition of
how immunohistochemistry was read should be provided as well as about serum measurements
of cytokines Does any comparison has performed to the ajustent 'normal' mucosa?  Authors
should define clinical and demographic parameters recorded Why smoking habits and alcohol
consumption were excluded? Duration of IBD is useful to be reported Fecal calprotectin
numerical value instead of qualitative evaluation should be provided IBD activity measurement
should also be provideded using an accepted scale Statistics ANOVA is the appropriate statistical
method to campare 3 variables and not Mann-Whitney U test Results ROC curves is better to
be calculated between presence of various markers and the activity index It would be useful that
the authors might provide representative immunohistochemistry pictures
Title: Correlation between PDGF-BB and M1-type macrophage in inflammatory bowel disease:
a case-control study
Version: 1 Date: 30 Sep 2024
Reviewer:  Panagiotis Tsibouris
Reviewer's report:
Abstract results reporting should define serum and colonic measurements  They should report
the levels of PDGF in all patients and not in a small subgroup Methods Why authors selected
a 12 week instead of 24 or 48 week time to perform colonoscopy as most guidelines suggest
in order to verify remission Were there any inclusion or exclusion criteria apart from the
underlined disease, such as age, presence of comorbidities, presence of neoplasia, pregnancy
and lactation? Authors report that they sample the same area after entering remission How thay
have accomplish this task because any marking might have influenced sampling? Definition of
how immunohistochemistry was read should be provided as well as about serum measurements
of cytokines Does any comparison has performed to the ajustent 'normal' mucosa?  Authors
should define clinical and demographic parameters recorded Why smoking habits and alcohol
consumption were excluded? Duration of IBD is useful to be reported Fecal calprotectin
numerical value instead of qualitative evaluation should be provided IBD activity measurement
should also be provideded using an accepted scale Statistics ANOVA is the appropriate statistical
method to campare 3 variables and not Mann-Whitney U test Results ROC curves is better to
be calculated between presence of various markers and the activity index It would be useful that
the authors might provide representative immunohistochemistry pictures
Please review this paper.
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Title: Correlation between PDGF-BB and M1-type macrophage in inflammatory bowel disease:
a case-control study
Version: 1 Date: 30 Sep 2024
Reviewer:  Panagiotis Tsibouris
Reviewer's report:
Abstract results reporting should define serum and colonic measurements  They should report
the levels of PDGF in all patients and not in a small subgroup Methods Why authors selected
a 12 week instead of 24 or 48 week time to perform colonoscopy as most guidelines suggest
in order to verify remission Were there any inclusion or exclusion criteria apart from the
underlined disease, such as age, presence of comorbidities, presence of neoplasia, pregnancy
and lactation? Authors report that they sample the same area after entering remission How thay
have accomplish this task because any marking might have influenced sampling? Definition of
how immunohistochemistry was read should be provided as well as about serum measurements
of cytokines Does any comparison has performed to the ajustent 'normal' mucosa?  Authors
should define clinical and demographic parameters recorded Why smoking habits and alcohol
consumption were excluded? Duration of IBD is useful to be reported Fecal calprotectin
numerical value instead of qualitative evaluation should be provided IBD activity measurement
should also be provideded using an accepted scale Statistics ANOVA is the appropriate statistical
method to campare 3 variables and not Mann-Whitney U test Results ROC curves is better to
be calculated between presence of various markers and the activity index It would be useful that
the authors might provide representative immunohistochemistry pictures
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