Title: ZBTB10 as a potential prognosis biomarker and correlates with tumor immune
microenvironment in stomach adenocarcinoma
Version: 1 Date: 09 Feb 2025
Reviewer's report:
In this study, Jiang and colleagues investigated the role of ZBTB10 in gastric cancer. They
identified that ZEBTB10 was overexpressed in GC and predicted a poor prognosis. Also,
ZEBTB10 could be a prognosis biomarker in GC. In addition, they found a close correlation
between ZEBTB10 expression and microenvironment. In vitro experiments revealed that the
capacities of tumor cells proliferation, organoid morphologically area and migration ability were
significantly inhibited after ZBTB10 knockdown. In general, this work is interesting and has
innovation. However, some major issues need to be addressed before publication.
1. The figure 1B in results should be placed to supplementary files, as it did not provide effective
information.
2.  What is the difference between Figure 1A and Figure 2A. These results are duplicated.
3. The description “significantly upregulated” in Figure 2D is wrong, as only one case could
not conduct statistical analysis.
4. In Figure 3, it is necessary to specify between which two groups the comparison shows
significance with the significance symbols.
5. The authors should investigate the role of ZBTB10 in EMT signaling of gastric cancer cells,
as knockdown of ZBTB10 inhibited cell migaration. I think this reference (36808651) may be
helpful to you.
6. Some key references (38806480, 38479160, 36681667) regarding on tumor
microenvironment and metastasis should be included.
Title: ZBTB10 as a potential prognosis biomarker and correlates with tumor immune
microenvironment in stomach adenocarcinoma
Version: 1 Date: 09 Feb 2025
Reviewer's report:
In this study, Jiang and colleagues investigated the role of ZBTB10 in gastric cancer. They
identified that ZEBTB10 was overexpressed in GC and predicted a poor prognosis. Also,
ZEBTB10 could be a prognosis biomarker in GC. In addition, they found a close correlation
between ZEBTB10 expression and microenvironment. In vitro experiments revealed that the
capacities of tumor cells proliferation, organoid morphologically area and migration ability were
significantly inhibited after ZBTB10 knockdown. In general, this work is interesting and has
innovation. However, some major issues need to be addressed before publication.
1. The figure 1B in results should be placed to supplementary files, as it did not provide effective
information.
2.  What is the difference between Figure 1A and Figure 2A. These results are duplicated.
3. The description “significantly upregulated” in Figure 2D is wrong, as only one case could
not conduct statistical analysis.
4. In Figure 3, it is necessary to specify between which two groups the comparison shows
significance with the significance symbols.
5. The authors should investigate the role of ZBTB10 in EMT signaling of gastric cancer cells,
as knockdown of ZBTB10 inhibited cell migaration. I think this reference (36808651) may be
helpful to you.
6. Some key references (38806480, 38479160, 36681667) regarding on tumor
microenvironment and metastasis should be included.
Please review this paper.
user
Title: ZBTB10 as a potential prognosis biomarker and correlates with tumor immune
microenvironment in stomach adenocarcinoma
Version: 1 Date: 09 Feb 2025
Reviewer's report:
In this study, Jiang and colleagues investigated the role of ZBTB10 in gastric cancer. They
identified that ZEBTB10 was overexpressed in GC and predicted a poor prognosis. Also,
ZEBTB10 could be a prognosis biomarker in GC. In addition, they found a close correlation
between ZEBTB10 expression and microenvironment. In vitro experiments revealed that the
capacities of tumor cells proliferation, organoid morphologically area and migration ability were
significantly inhibited after ZBTB10 knockdown. In general, this work is interesting and has
innovation. However, some major issues need to be addressed before publication.
1. The figure 1B in results should be placed to supplementary files, as it did not provide effective
information.
2.  What is the difference between Figure 1A and Figure 2A. These results are duplicated.
3. The description “significantly upregulated” in Figure 2D is wrong, as only one case could
not conduct statistical analysis.
4. In Figure 3, it is necessary to specify between which two groups the comparison shows
significance with the significance symbols.
5. The authors should investigate the role of ZBTB10 in EMT signaling of gastric cancer cells,
as knockdown of ZBTB10 inhibited cell migaration. I think this reference (36808651) may be
helpful to you.
6. Some key references (38806480, 38479160, 36681667) regarding on tumor
microenvironment and metastasis should be included.
assistant