Reviewer's report
Title:Palm Vitamin E Reduces Catecholamines, Xanthine Oxidase Activity and
Gastric Lesions in Rats Exposed To Water-Immersion Restraint Stress
Version: 1Date:21 November 2011
Reviewer: Lori A Coburn
Reviewer's report:
Major Compulsory Revisions – None related to the basic scientific procedures
Minor Essential Revisions
1. Please describe why the dose of palm vitamin E and alpha-tocopherol was
chosen to be 60mg/kg a day? Why was it given for 28 days prior to stress? How
does this dose relate to the amount present in the regular diet?
2. Olive oil was given as the vehicle – olive oil does contain some vitamin E –
approx. 1.6mg per tablespoon – it is not clear how much olive oil was given every
day though it was likely a small amount.
3. In the abstract – “Sixty male Sprague-Dawley rats (200-250g) were randomly
divided into four equal sized groups.” – should be three equal sized groups.
4. “Although tocopherol is well known to be the most abundant and active form of
vitamin E, but recently the role of tocotrienols has received renewed attention.” It
may be the most studied though I am not sure if it can be said to the most
abundant.
5. Page 8 – “We had previously reported that the increase in the noradrenalin
level was blocked in rats given TT supplementation but not in rats receiving #-TF”
define TT. Why was TT used in the prior experiments and palm vitamin E in this
experiment?
6. The references do not all have the same formatting – eg #5 – bold and italics
missing
7. I would recommend the authors work to clarify some of the English syntax in
the manuscript
Discretionary Revisions
1. Representative photographs of the gastric mucosal lesions would be helpful to
supplement Figure 1.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:Palm Vitamin E Reduces Catecholamines, Xanthine Oxidase Activity and
Gastric Lesions in Rats Exposed To Water-Immersion Restraint Stress
Version: 1Date:21 November 2011
Reviewer: Lori A Coburn
Reviewer's report:
Major Compulsory Revisions – None related to the basic scientific procedures
Minor Essential Revisions
1. Please describe why the dose of palm vitamin E and alpha-tocopherol was
chosen to be 60mg/kg a day? Why was it given for 28 days prior to stress? How
does this dose relate to the amount present in the regular diet?
2. Olive oil was given as the vehicle – olive oil does contain some vitamin E –
approx. 1.6mg per tablespoon – it is not clear how much olive oil was given every
day though it was likely a small amount.
3. In the abstract – “Sixty male Sprague-Dawley rats (200-250g) were randomly
divided into four equal sized groups.” – should be three equal sized groups.
4. “Although tocopherol is well known to be the most abundant and active form of
vitamin E, but recently the role of tocotrienols has received renewed attention.” It
may be the most studied though I am not sure if it can be said to the most
abundant.
5. Page 8 – “We had previously reported that the increase in the noradrenalin
level was blocked in rats given TT supplementation but not in rats receiving #-TF”
define TT. Why was TT used in the prior experiments and palm vitamin E in this
experiment?
6. The references do not all have the same formatting – eg #5 – bold and italics
missing
7. I would recommend the authors work to clarify some of the English syntax in
the manuscript
Discretionary Revisions
1. Representative photographs of the gastric mucosal lesions would be helpful to
supplement Figure 1.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:Palm Vitamin E Reduces Catecholamines, Xanthine Oxidase Activity and
Gastric Lesions in Rats Exposed To Water-Immersion Restraint Stress
Version: 1Date:21 November 2011
Reviewer: Lori A Coburn
Reviewer's report:
Major Compulsory Revisions – None related to the basic scientific procedures
Minor Essential Revisions
1. Please describe why the dose of palm vitamin E and alpha-tocopherol was
chosen to be 60mg/kg a day? Why was it given for 28 days prior to stress? How
does this dose relate to the amount present in the regular diet?
2. Olive oil was given as the vehicle – olive oil does contain some vitamin E –
approx. 1.6mg per tablespoon – it is not clear how much olive oil was given every
day though it was likely a small amount.
3. In the abstract – “Sixty male Sprague-Dawley rats (200-250g) were randomly
divided into four equal sized groups.” – should be three equal sized groups.
4. “Although tocopherol is well known to be the most abundant and active form of
vitamin E, but recently the role of tocotrienols has received renewed attention.” It
may be the most studied though I am not sure if it can be said to the most
abundant.
5. Page 8 – “We had previously reported that the increase in the noradrenalin
level was blocked in rats given TT supplementation but not in rats receiving #-TF”
define TT. Why was TT used in the prior experiments and palm vitamin E in this
experiment?
6. The references do not all have the same formatting – eg #5 – bold and italics
missing
7. I would recommend the authors work to clarify some of the English syntax in
the manuscript
Discretionary Revisions
1. Representative photographs of the gastric mucosal lesions would be helpful to
supplement Figure 1.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
assistant