Title: The hsa_circ_0039857/miR-338-3p/RAB32 axis promotes the malignant progression of
colorectal cancer
Version: 1 Date: 21 Jun 2022
Reviewer's report:
• In  the sentence " The targeting relationship between molecules was then analyzed by
bioinformatics." And " Surprisingly, bioinformatics analysis revealed that circ_0039857 binds
to miR-338-3p (Fig. 5A)." the authors mentioned the bioinformatics analysis. Unfortunately, I
couldn't find in detail material and method and results for bioinformatics analysis. Please provide
the related sections .
• In the introduction section, the authors didn't describe how they select circ_0039857 ,
miR-338-3p, RAB32  for evaluation in detail.   The authors provide general information about
circ-RNAs, miRNAs, and mRNAs . please mention more detail about selected circ-RNA,
miRNA, and mRNA.
• The country of each product should be mentioned in addition to the company name for example:
"The cells were cultured in RPMI-1640 medium (Gibco, USA) with 10% FBS (Gibco,USA),
1% penicillin and streptomycin (Gibco, USA) solution."
• in the sentence "GAPDH and U6 were set as the internal parameters for mRNA and miRNA."
please declare that for normalization of circ-RNA , miRNA, and mRNA which internal was used
separately.  Was the GAPDH used for circ-RNA normalization?
• In table 2 please provide the accession number of circ-RNA, miRNA, and mRNA.
• In the sentence "Each group of cells (300 cells) was added to a 6-well plate and cultured two
weeks" please declare that if all used cell lines were seeded in equal density and evaluated at
equal times (two weeks).
Title: The hsa_circ_0039857/miR-338-3p/RAB32 axis promotes the malignant progression of
colorectal cancer
Version: 1 Date: 21 Jun 2022
Reviewer's report:
• In  the sentence " The targeting relationship between molecules was then analyzed by
bioinformatics." And " Surprisingly, bioinformatics analysis revealed that circ_0039857 binds
to miR-338-3p (Fig. 5A)." the authors mentioned the bioinformatics analysis. Unfortunately, I
couldn't find in detail material and method and results for bioinformatics analysis. Please provide
the related sections .
• In the introduction section, the authors didn't describe how they select circ_0039857 ,
miR-338-3p, RAB32  for evaluation in detail.   The authors provide general information about
circ-RNAs, miRNAs, and mRNAs . please mention more detail about selected circ-RNA,
miRNA, and mRNA.
• The country of each product should be mentioned in addition to the company name for example:
"The cells were cultured in RPMI-1640 medium (Gibco, USA) with 10% FBS (Gibco,USA),
1% penicillin and streptomycin (Gibco, USA) solution."
• in the sentence "GAPDH and U6 were set as the internal parameters for mRNA and miRNA."
please declare that for normalization of circ-RNA , miRNA, and mRNA which internal was used
separately.  Was the GAPDH used for circ-RNA normalization?
• In table 2 please provide the accession number of circ-RNA, miRNA, and mRNA.
• In the sentence "Each group of cells (300 cells) was added to a 6-well plate and cultured two
weeks" please declare that if all used cell lines were seeded in equal density and evaluated at
equal times (two weeks).
Please review this paper.
user
Title: The hsa_circ_0039857/miR-338-3p/RAB32 axis promotes the malignant progression of
colorectal cancer
Version: 1 Date: 21 Jun 2022
Reviewer's report:
• In  the sentence " The targeting relationship between molecules was then analyzed by
bioinformatics." And " Surprisingly, bioinformatics analysis revealed that circ_0039857 binds
to miR-338-3p (Fig. 5A)." the authors mentioned the bioinformatics analysis. Unfortunately, I
couldn't find in detail material and method and results for bioinformatics analysis. Please provide
the related sections .
• In the introduction section, the authors didn't describe how they select circ_0039857 ,
miR-338-3p, RAB32  for evaluation in detail.   The authors provide general information about
circ-RNAs, miRNAs, and mRNAs . please mention more detail about selected circ-RNA,
miRNA, and mRNA.
• The country of each product should be mentioned in addition to the company name for example:
"The cells were cultured in RPMI-1640 medium (Gibco, USA) with 10% FBS (Gibco,USA),
1% penicillin and streptomycin (Gibco, USA) solution."
• in the sentence "GAPDH and U6 were set as the internal parameters for mRNA and miRNA."
please declare that for normalization of circ-RNA , miRNA, and mRNA which internal was used
separately.  Was the GAPDH used for circ-RNA normalization?
• In table 2 please provide the accession number of circ-RNA, miRNA, and mRNA.
• In the sentence "Each group of cells (300 cells) was added to a 6-well plate and cultured two
weeks" please declare that if all used cell lines were seeded in equal density and evaluated at
equal times (two weeks).
assistant