Title: Endoscopic Ultrasound Guided Fine Needle Biopsy (EUS-FNB) from Peritoneal Lesions:
A Prospective Cohort Pilot Study
Version: 1 Date: 15 Jul 2021
Reviewer's report:
In this prospective study, the authors evaluated the yield of EUS-FNB for the diagnosis of
peritoneal lesions. The topic is interesting and not fully investigated so far.
Here my comments:
- Please add this reference (PMID: 29941722) after this sentence in the introduction section:
“Nevertheless, it is not practical to use tissue from EUS-FNA for IHC staining due to the
relatively small volume of tissue collected compared to a EUS-guided fine needle biopsy (EUS-
FNB)”.
- In Table 2, please indicate the diagnostic yield of abdominal paracentesis.
- In your study, you used a large bore, side-fenestrated needle (20 gauge). To me, this needle
is not the optimal one for sampling small peritoneal lesions. This point could explain the low
sensitivity you obtained in your study (63%). Moreover, recent studies have demonstrated that
new generation end-cutting needles (e.g., Fork-tip and Franseen) outperformed side fenestrated
ones. Please discuss this point by adding these references
(PMID: 34116031, PMID: 32433914)
- A novel technique for peritoneal sampling has been recently proposed (Moray microforceps).
This tool demonstrated to be able to achieve a definitive diagnosis in more than 70% of cases.
Please discuss this point by adding these references (PMID: 33765374, PMID: 31323232).
Title: Endoscopic Ultrasound Guided Fine Needle Biopsy (EUS-FNB) from Peritoneal Lesions:
A Prospective Cohort Pilot Study
Version: 1 Date: 15 Jul 2021
Reviewer's report:
In this prospective study, the authors evaluated the yield of EUS-FNB for the diagnosis of
peritoneal lesions. The topic is interesting and not fully investigated so far.
Here my comments:
- Please add this reference (PMID: 29941722) after this sentence in the introduction section:
“Nevertheless, it is not practical to use tissue from EUS-FNA for IHC staining due to the
relatively small volume of tissue collected compared to a EUS-guided fine needle biopsy (EUS-
FNB)”.
- In Table 2, please indicate the diagnostic yield of abdominal paracentesis.
- In your study, you used a large bore, side-fenestrated needle (20 gauge). To me, this needle
is not the optimal one for sampling small peritoneal lesions. This point could explain the low
sensitivity you obtained in your study (63%). Moreover, recent studies have demonstrated that
new generation end-cutting needles (e.g., Fork-tip and Franseen) outperformed side fenestrated
ones. Please discuss this point by adding these references
(PMID: 34116031, PMID: 32433914)
- A novel technique for peritoneal sampling has been recently proposed (Moray microforceps).
This tool demonstrated to be able to achieve a definitive diagnosis in more than 70% of cases.
Please discuss this point by adding these references (PMID: 33765374, PMID: 31323232).
Please review this paper.
user
Title: Endoscopic Ultrasound Guided Fine Needle Biopsy (EUS-FNB) from Peritoneal Lesions:
A Prospective Cohort Pilot Study
Version: 1 Date: 15 Jul 2021
Reviewer's report:
In this prospective study, the authors evaluated the yield of EUS-FNB for the diagnosis of
peritoneal lesions. The topic is interesting and not fully investigated so far.
Here my comments:
- Please add this reference (PMID: 29941722) after this sentence in the introduction section:
“Nevertheless, it is not practical to use tissue from EUS-FNA for IHC staining due to the
relatively small volume of tissue collected compared to a EUS-guided fine needle biopsy (EUS-
FNB)”.
- In Table 2, please indicate the diagnostic yield of abdominal paracentesis.
- In your study, you used a large bore, side-fenestrated needle (20 gauge). To me, this needle
is not the optimal one for sampling small peritoneal lesions. This point could explain the low
sensitivity you obtained in your study (63%). Moreover, recent studies have demonstrated that
new generation end-cutting needles (e.g., Fork-tip and Franseen) outperformed side fenestrated
ones. Please discuss this point by adding these references
(PMID: 34116031, PMID: 32433914)
- A novel technique for peritoneal sampling has been recently proposed (Moray microforceps).
This tool demonstrated to be able to achieve a definitive diagnosis in more than 70% of cases.
Please discuss this point by adding these references (PMID: 33765374, PMID: 31323232).
assistant