Title: Analysis of the effect of fecal SDC2, ADHFE1 and PPP2R5C gene methylation test for
screening colorectal cancer in the Otog Front Banner
Version: 0 Date: 14 Nov 2024
Reviewer:  Konosuke Nakaji
Reviewer's report:
The article has described the usefulness of stool DNA testing for screening CRC programs.
The author stated that a combination of SDC2, ADHFE1, and PPP2R5C gene methylation
and subsequent colonoscopy for each positive patient is helpful for CRC screening. This is
an interesting article. However, there are some concerns. 1. In the introduction, the authors
described DNA methylation assay has become a standard tool for early screening and diagnosis
of CRC. Is this firm evidence? 2. In the material and methods section, the authors should briefly
describe how to analyze the stool, such as real-time PCR amplification for the SDC2 gene. 3.
The authors should discuss the low compliance rate of about 60%. 4. What kinds of colonoscopy
were used? Who is diagnosed with CRC? 5. A table needs to be remade due to the lack of
explanation of each factor [e.g., Age (years old)], and Table five needs to correct the verb as
P and #2-value.
Title: Analysis of the effect of fecal SDC2, ADHFE1 and PPP2R5C gene methylation test for
screening colorectal cancer in the Otog Front Banner
Version: 0 Date: 14 Nov 2024
Reviewer:  Konosuke Nakaji
Reviewer's report:
The article has described the usefulness of stool DNA testing for screening CRC programs.
The author stated that a combination of SDC2, ADHFE1, and PPP2R5C gene methylation
and subsequent colonoscopy for each positive patient is helpful for CRC screening. This is
an interesting article. However, there are some concerns. 1. In the introduction, the authors
described DNA methylation assay has become a standard tool for early screening and diagnosis
of CRC. Is this firm evidence? 2. In the material and methods section, the authors should briefly
describe how to analyze the stool, such as real-time PCR amplification for the SDC2 gene. 3.
The authors should discuss the low compliance rate of about 60%. 4. What kinds of colonoscopy
were used? Who is diagnosed with CRC? 5. A table needs to be remade due to the lack of
explanation of each factor [e.g., Age (years old)], and Table five needs to correct the verb as
P and #2-value.
Please review this paper.
user
Title: Analysis of the effect of fecal SDC2, ADHFE1 and PPP2R5C gene methylation test for
screening colorectal cancer in the Otog Front Banner
Version: 0 Date: 14 Nov 2024
Reviewer:  Konosuke Nakaji
Reviewer's report:
The article has described the usefulness of stool DNA testing for screening CRC programs.
The author stated that a combination of SDC2, ADHFE1, and PPP2R5C gene methylation
and subsequent colonoscopy for each positive patient is helpful for CRC screening. This is
an interesting article. However, there are some concerns. 1. In the introduction, the authors
described DNA methylation assay has become a standard tool for early screening and diagnosis
of CRC. Is this firm evidence? 2. In the material and methods section, the authors should briefly
describe how to analyze the stool, such as real-time PCR amplification for the SDC2 gene. 3.
The authors should discuss the low compliance rate of about 60%. 4. What kinds of colonoscopy
were used? Who is diagnosed with CRC? 5. A table needs to be remade due to the lack of
explanation of each factor [e.g., Age (years old)], and Table five needs to correct the verb as
P and #2-value.
assistant