Title: Expression of 5-hydroxytryptamine 7 receptor in intestinal mucosa correlates with the
degree of intestinal inflammation in Crohn's disease
Version: 1 Date: 22 Jul 2022
Reviewer's report:
Xu et al. reported that the expression of 5-hydroxytryptamine 7 receptor predicts the degree of
intestinal inflammation in Crohn´s Disease patients. The topic of the manuscript is quite relevant
since it points to a possible biomarker of the severity of these patients. For that reason, it includes
the analysis of a sufficient number of patients in both active and remission status, with a control
group without the disease. In spite of the fact that the manuscript includes enough number of
experiments to reach the main conclusion, for this reviewer there are some major concerns that
need to be addressed in order to be published in this journal.
- In the introduction, authors should explain more about the role of the receptor 5-HT7R in IBD.
They should also include all the results published in vitro and in vivo in order to highlight the
importance of this receptor.
- In Methods Section, authors mention immunohistochemistry, whereas they have performed
immunofluorescence. They should include the name of the specific fluorophores used. Authors
should repeat the experiment with a negative control in order to discard the autofluorescence
and also with Hoechst in order to show the nuclei and the architecture of the tissue analysed.
- Authors state that there is no protein in control patients but they have detected the gene
expression. How can they explain these discrepancies?
- Authors only show the expression of the receptor in Cd11c cells. Is it not expressed in any
other cell type?
- In all the correlations of the manuscript, authors have included the control group. The
correlations must be repeated only with CD patients in order to demonstrate that this receptor
positively correlates with intestinal inflammation.
Title: Expression of 5-hydroxytryptamine 7 receptor in intestinal mucosa correlates with the
degree of intestinal inflammation in Crohn's disease
Version: 1 Date: 22 Jul 2022
Reviewer's report:
Xu et al. reported that the expression of 5-hydroxytryptamine 7 receptor predicts the degree of
intestinal inflammation in Crohn´s Disease patients. The topic of the manuscript is quite relevant
since it points to a possible biomarker of the severity of these patients. For that reason, it includes
the analysis of a sufficient number of patients in both active and remission status, with a control
group without the disease. In spite of the fact that the manuscript includes enough number of
experiments to reach the main conclusion, for this reviewer there are some major concerns that
need to be addressed in order to be published in this journal.
- In the introduction, authors should explain more about the role of the receptor 5-HT7R in IBD.
They should also include all the results published in vitro and in vivo in order to highlight the
importance of this receptor.
- In Methods Section, authors mention immunohistochemistry, whereas they have performed
immunofluorescence. They should include the name of the specific fluorophores used. Authors
should repeat the experiment with a negative control in order to discard the autofluorescence
and also with Hoechst in order to show the nuclei and the architecture of the tissue analysed.
- Authors state that there is no protein in control patients but they have detected the gene
expression. How can they explain these discrepancies?
- Authors only show the expression of the receptor in Cd11c cells. Is it not expressed in any
other cell type?
- In all the correlations of the manuscript, authors have included the control group. The
correlations must be repeated only with CD patients in order to demonstrate that this receptor
positively correlates with intestinal inflammation.
Please review this paper.
user
Title: Expression of 5-hydroxytryptamine 7 receptor in intestinal mucosa correlates with the
degree of intestinal inflammation in Crohn's disease
Version: 1 Date: 22 Jul 2022
Reviewer's report:
Xu et al. reported that the expression of 5-hydroxytryptamine 7 receptor predicts the degree of
intestinal inflammation in Crohn´s Disease patients. The topic of the manuscript is quite relevant
since it points to a possible biomarker of the severity of these patients. For that reason, it includes
the analysis of a sufficient number of patients in both active and remission status, with a control
group without the disease. In spite of the fact that the manuscript includes enough number of
experiments to reach the main conclusion, for this reviewer there are some major concerns that
need to be addressed in order to be published in this journal.
- In the introduction, authors should explain more about the role of the receptor 5-HT7R in IBD.
They should also include all the results published in vitro and in vivo in order to highlight the
importance of this receptor.
- In Methods Section, authors mention immunohistochemistry, whereas they have performed
immunofluorescence. They should include the name of the specific fluorophores used. Authors
should repeat the experiment with a negative control in order to discard the autofluorescence
and also with Hoechst in order to show the nuclei and the architecture of the tissue analysed.
- Authors state that there is no protein in control patients but they have detected the gene
expression. How can they explain these discrepancies?
- Authors only show the expression of the receptor in Cd11c cells. Is it not expressed in any
other cell type?
- In all the correlations of the manuscript, authors have included the control group. The
correlations must be repeated only with CD patients in order to demonstrate that this receptor
positively correlates with intestinal inflammation.
assistant