Reviewer's report
Title:Mitochondrial Uncouplers Inhibit Hepatic Stellate Cell Activation
Version: 1Date:29 February 2012
Reviewer: Margarete Odenthal
Reviewer's report:
The authors describe the impact of mitochondrial uncoupling on culture-induced
HSC activation and their response to pro-fibrogenic cytokines like TGF-#. This
study is of high interest, because the authors show inhibition of profibrogenic
features by mitochondrial uncoupling. This knowledge might be useful
concerning future therapeutic approaches.
Level of interest: An article of outstanding merit and interest in its field
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
Declaration of competing interests:
The authors describe the impact of mitochondrial uncoupling on culture-induced
HSC activation and the response to TGF-#. This study is of high interest,
because the authors show inhibition of profibrogenic features by mitochondrial
uncoupling in stellate cells. This knowledge might be useful concerning future
therapeutic approaches. The manuscript is well written and the study includes a
wide range of sophisticated technologies such as stellate cell preparation from
human liver tissues and many others.
There is only a minor objection.
Minor comment:
The reviewer just asks the authors to correct the title of table 1 into:
Table 1: Primer sequences, probes and accession numbers of transcripts, used
for RT PCR quantification.
Further, the reviewer suggests that the authors should show this table as a
supplemental table.
Reviewer's report
Title:Mitochondrial Uncouplers Inhibit Hepatic Stellate Cell Activation
Version: 1Date:29 February 2012
Reviewer: Margarete Odenthal
Reviewer's report:
The authors describe the impact of mitochondrial uncoupling on culture-induced
HSC activation and their response to pro-fibrogenic cytokines like TGF-#. This
study is of high interest, because the authors show inhibition of profibrogenic
features by mitochondrial uncoupling. This knowledge might be useful
concerning future therapeutic approaches.
Level of interest: An article of outstanding merit and interest in its field
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
Declaration of competing interests:
The authors describe the impact of mitochondrial uncoupling on culture-induced
HSC activation and the response to TGF-#. This study is of high interest,
because the authors show inhibition of profibrogenic features by mitochondrial
uncoupling in stellate cells. This knowledge might be useful concerning future
therapeutic approaches. The manuscript is well written and the study includes a
wide range of sophisticated technologies such as stellate cell preparation from
human liver tissues and many others.
There is only a minor objection.
Minor comment:
The reviewer just asks the authors to correct the title of table 1 into:
Table 1: Primer sequences, probes and accession numbers of transcripts, used
for RT PCR quantification.
Further, the reviewer suggests that the authors should show this table as a
supplemental table.
Please review this paper.
user
Reviewer's report
Title:Mitochondrial Uncouplers Inhibit Hepatic Stellate Cell Activation
Version: 1Date:29 February 2012
Reviewer: Margarete Odenthal
Reviewer's report:
The authors describe the impact of mitochondrial uncoupling on culture-induced
HSC activation and their response to pro-fibrogenic cytokines like TGF-#. This
study is of high interest, because the authors show inhibition of profibrogenic
features by mitochondrial uncoupling. This knowledge might be useful
concerning future therapeutic approaches.
Level of interest: An article of outstanding merit and interest in its field
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
Declaration of competing interests:
The authors describe the impact of mitochondrial uncoupling on culture-induced
HSC activation and the response to TGF-#. This study is of high interest,
because the authors show inhibition of profibrogenic features by mitochondrial
uncoupling in stellate cells. This knowledge might be useful concerning future
therapeutic approaches. The manuscript is well written and the study includes a
wide range of sophisticated technologies such as stellate cell preparation from
human liver tissues and many others.
There is only a minor objection.
Minor comment:
The reviewer just asks the authors to correct the title of table 1 into:
Table 1: Primer sequences, probes and accession numbers of transcripts, used
for RT PCR quantification.
Further, the reviewer suggests that the authors should show this table as a
supplemental table.
assistant