Reviewer's report 
 Effects of oral adenosine 5-'triphosphate and adenosine in enteric-coated capsules on Title: 
indomethacin-induced permeability changes in the human small intestine: a randomized cross-over study 
 1  4 April 2007 Version: Date: 
 Martin Gotteland Reviewer: 
Reviewer's report: 
------------------------------------------------------------------------------- 
Discretionary Revisions (which the author can choose to ignore) 
The present study was carried out to evaluate the effect of the oral administration of encapsulated 
adenosine or ATP on indomethacin-induced alterations of the intestinal barrier function. It was based on the previous observation by the authors that topical application of ATP through naso-intestinal tube normalized intestinal permeability to lactulose/rhamnose. 
The objective is well defined, the methods are appropriate and the data are well controlled and support 
adequately the discussion and conclusions. 
The relevance of the study, however, is limited due to the negative results obtained: no protective effects of 
ATP or adenosine were observed when these compounds were administered as enteric-coated capsules. 
As explained by the authors, this is probably due to the fact that indomethacin acts on the upper segments 
of the small intestine while the opening of the Eudragit capsules occurs at a more distal site. This means 
that the authors have to use another model of gut barrier alteration affecting the intestine more distally, or to use another type of coating allowing the release of the drug in the upper intestine. 
 Reject because too small an advance to publish What next?: 
 An article of limited interest Level of interest: 
 Acceptable Quality of written English: 
 No, the manuscript does not need to be seen by a statistician. Statistical review: 
Declaration of competing interests: 
I declare that I have no competing interests 
Reviewer's report 
 Effects of oral adenosine 5-'triphosphate and adenosine in enteric-coated capsules on Title: 
indomethacin-induced permeability changes in the human small intestine: a randomized cross-over study 
 1  4 April 2007 Version: Date: 
 Martin Gotteland Reviewer: 
Reviewer's report: 
------------------------------------------------------------------------------- 
Discretionary Revisions (which the author can choose to ignore) 
The present study was carried out to evaluate the effect of the oral administration of encapsulated 
adenosine or ATP on indomethacin-induced alterations of the intestinal barrier function. It was based on the previous observation by the authors that topical application of ATP through naso-intestinal tube normalized intestinal permeability to lactulose/rhamnose. 
The objective is well defined, the methods are appropriate and the data are well controlled and support 
adequately the discussion and conclusions. 
The relevance of the study, however, is limited due to the negative results obtained: no protective effects of 
ATP or adenosine were observed when these compounds were administered as enteric-coated capsules. 
As explained by the authors, this is probably due to the fact that indomethacin acts on the upper segments 
of the small intestine while the opening of the Eudragit capsules occurs at a more distal site. This means 
that the authors have to use another model of gut barrier alteration affecting the intestine more distally, or to use another type of coating allowing the release of the drug in the upper intestine. 
 Reject because too small an advance to publish What next?: 
 An article of limited interest Level of interest: 
 Acceptable Quality of written English: 
 No, the manuscript does not need to be seen by a statistician. Statistical review: 
Declaration of competing interests: 
I declare that I have no competing interests 
Please review this paper.
user
Reviewer's report 
 Effects of oral adenosine 5-'triphosphate and adenosine in enteric-coated capsules on Title: 
indomethacin-induced permeability changes in the human small intestine: a randomized cross-over study 
 1  4 April 2007 Version: Date: 
 Martin Gotteland Reviewer: 
Reviewer's report: 
------------------------------------------------------------------------------- 
Discretionary Revisions (which the author can choose to ignore) 
The present study was carried out to evaluate the effect of the oral administration of encapsulated 
adenosine or ATP on indomethacin-induced alterations of the intestinal barrier function. It was based on the previous observation by the authors that topical application of ATP through naso-intestinal tube normalized intestinal permeability to lactulose/rhamnose. 
The objective is well defined, the methods are appropriate and the data are well controlled and support 
adequately the discussion and conclusions. 
The relevance of the study, however, is limited due to the negative results obtained: no protective effects of 
ATP or adenosine were observed when these compounds were administered as enteric-coated capsules. 
As explained by the authors, this is probably due to the fact that indomethacin acts on the upper segments 
of the small intestine while the opening of the Eudragit capsules occurs at a more distal site. This means 
that the authors have to use another model of gut barrier alteration affecting the intestine more distally, or to use another type of coating allowing the release of the drug in the upper intestine. 
 Reject because too small an advance to publish What next?: 
 An article of limited interest Level of interest: 
 Acceptable Quality of written English: 
 No, the manuscript does not need to be seen by a statistician. Statistical review: 
Declaration of competing interests: 
I declare that I have no competing interests 
assistant