Reviewer's report
Title:Lymphatic marker podoplanin/D2-40 in human advanced cirrhotic liver-
Reevaluations of microlymphatic abnormalities
Version: 1Date:29 August 2010
Reviewer: Armin Thelen
Reviewer's report:
The present manuscript describes a study about the specifity of the monoclonal
antibody D2-40 to stain lymphatic vessels in human liver tissue. Furthermore, it
analyzes the quantity of lymphatic vessels in normal and cirrhotic liver
specimens. In general, the subject of the manuscript is interesting and it is well
structured and well written. However, there are some issues which may be
addressed prior to publication.
Minor:
1. The most critisism of the study is the low number of analyzed tissue
specimens (only 5 in each group). That is not important for the analyzation of the
speciity of the antibody but it is a disadvantage for the analyzation and the
comparison of the vesel density between the different study groups. Therefore, it
may be helpful to adress this shortcoming of the study in the discussion section
2. First paragraph of the section “Materials and methods”: It is not clear to me
which groups of tissue speimens come from patients wh underwent resection
and who underwent liver transplantation as both are described as tissues from
cirrhoic livers. Authors may specify this.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Lymphatic marker podoplanin/D2-40 in human advanced cirrhotic liver-
Reevaluations of microlymphatic abnormalities
Version: 1Date:29 August 2010
Reviewer: Armin Thelen
Reviewer's report:
The present manuscript describes a study about the specifity of the monoclonal
antibody D2-40 to stain lymphatic vessels in human liver tissue. Furthermore, it
analyzes the quantity of lymphatic vessels in normal and cirrhotic liver
specimens. In general, the subject of the manuscript is interesting and it is well
structured and well written. However, there are some issues which may be
addressed prior to publication.
Minor:
1. The most critisism of the study is the low number of analyzed tissue
specimens (only 5 in each group). That is not important for the analyzation of the
speciity of the antibody but it is a disadvantage for the analyzation and the
comparison of the vesel density between the different study groups. Therefore, it
may be helpful to adress this shortcoming of the study in the discussion section
2. First paragraph of the section “Materials and methods”: It is not clear to me
which groups of tissue speimens come from patients wh underwent resection
and who underwent liver transplantation as both are described as tissues from
cirrhoic livers. Authors may specify this.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Lymphatic marker podoplanin/D2-40 in human advanced cirrhotic liver-
Reevaluations of microlymphatic abnormalities
Version: 1Date:29 August 2010
Reviewer: Armin Thelen
Reviewer's report:
The present manuscript describes a study about the specifity of the monoclonal
antibody D2-40 to stain lymphatic vessels in human liver tissue. Furthermore, it
analyzes the quantity of lymphatic vessels in normal and cirrhotic liver
specimens. In general, the subject of the manuscript is interesting and it is well
structured and well written. However, there are some issues which may be
addressed prior to publication.
Minor:
1. The most critisism of the study is the low number of analyzed tissue
specimens (only 5 in each group). That is not important for the analyzation of the
speciity of the antibody but it is a disadvantage for the analyzation and the
comparison of the vesel density between the different study groups. Therefore, it
may be helpful to adress this shortcoming of the study in the discussion section
2. First paragraph of the section “Materials and methods”: It is not clear to me
which groups of tissue speimens come from patients wh underwent resection
and who underwent liver transplantation as both are described as tissues from
cirrhoic livers. Authors may specify this.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
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