Title: Biochemical and histopathological evidence for beneficial effects of empagliflozin
pretreatment on acetic acid-induced colitis in rats
Version: 5 Date: 19 May 2023
Reviewer:  Paul Aimé Noubissi
Reviewer's report:
Biochemical and histopathological evidence for beneficial effects of empagliflozin pretreatment
on acetic acid-induced colitis in rats
This study aimed to validate the biochemical and histopathological evidence for beneficial
effects of empagliflozin pretreatment on acetic acid-induced colitis in rats. However, there are
some minor problems that need to be addressed.
Abstract
Results
- The abstract might present the only source of knowledge for many researchers who has no
access to the whole work, therefore it is highly recommended to add some digital results in the
abstract rather than elastic sentences. The authors should include values in the result section of
the abstract. At the moment, they are barely described.
Experimental design
- How did you measure the colon length?
Results
- On Fig 1., it was indicated that ## stands for p < 0.01 vs. AA group. How can you explain
that the Empa group with the weight-to-length ratio of 81.00 ± 5.28 mg/cm and the AA + Dexa
group with 81.80 ± 4.18 mg/cm were significantly different compared to AA group; and not the
control group with 58.80 ± 5.69 mg/cm?
- Fig 3 lacks the magnification power. It has to be written directly under the diagram.
Effect of empagliflozin on oxidative stress and antioxidant activity
- Why did you not evaluate the levels of some pro or anti-inflammatory cytokines in colonic
tissue homogenate?
Discussion
- “In the current study application of AA disturbed the colonic mucus” How did you evaluate
that? Because there was no result on the amount of colonic mucus!
- In general, English editing is required.
Title: Biochemical and histopathological evidence for beneficial effects of empagliflozin
pretreatment on acetic acid-induced colitis in rats
Version: 5 Date: 19 May 2023
Reviewer:  Paul Aimé Noubissi
Reviewer's report:
Biochemical and histopathological evidence for beneficial effects of empagliflozin pretreatment
on acetic acid-induced colitis in rats
This study aimed to validate the biochemical and histopathological evidence for beneficial
effects of empagliflozin pretreatment on acetic acid-induced colitis in rats. However, there are
some minor problems that need to be addressed.
Abstract
Results
- The abstract might present the only source of knowledge for many researchers who has no
access to the whole work, therefore it is highly recommended to add some digital results in the
abstract rather than elastic sentences. The authors should include values in the result section of
the abstract. At the moment, they are barely described.
Experimental design
- How did you measure the colon length?
Results
- On Fig 1., it was indicated that ## stands for p < 0.01 vs. AA group. How can you explain
that the Empa group with the weight-to-length ratio of 81.00 ± 5.28 mg/cm and the AA + Dexa
group with 81.80 ± 4.18 mg/cm were significantly different compared to AA group; and not the
control group with 58.80 ± 5.69 mg/cm?
- Fig 3 lacks the magnification power. It has to be written directly under the diagram.
Effect of empagliflozin on oxidative stress and antioxidant activity
- Why did you not evaluate the levels of some pro or anti-inflammatory cytokines in colonic
tissue homogenate?
Discussion
- “In the current study application of AA disturbed the colonic mucus” How did you evaluate
that? Because there was no result on the amount of colonic mucus!
- In general, English editing is required.
Please review this paper.
user
Title: Biochemical and histopathological evidence for beneficial effects of empagliflozin
pretreatment on acetic acid-induced colitis in rats
Version: 5 Date: 19 May 2023
Reviewer:  Paul Aimé Noubissi
Reviewer's report:
Biochemical and histopathological evidence for beneficial effects of empagliflozin pretreatment
on acetic acid-induced colitis in rats
This study aimed to validate the biochemical and histopathological evidence for beneficial
effects of empagliflozin pretreatment on acetic acid-induced colitis in rats. However, there are
some minor problems that need to be addressed.
Abstract
Results
- The abstract might present the only source of knowledge for many researchers who has no
access to the whole work, therefore it is highly recommended to add some digital results in the
abstract rather than elastic sentences. The authors should include values in the result section of
the abstract. At the moment, they are barely described.
Experimental design
- How did you measure the colon length?
Results
- On Fig 1., it was indicated that ## stands for p < 0.01 vs. AA group. How can you explain
that the Empa group with the weight-to-length ratio of 81.00 ± 5.28 mg/cm and the AA + Dexa
group with 81.80 ± 4.18 mg/cm were significantly different compared to AA group; and not the
control group with 58.80 ± 5.69 mg/cm?
- Fig 3 lacks the magnification power. It has to be written directly under the diagram.
Effect of empagliflozin on oxidative stress and antioxidant activity
- Why did you not evaluate the levels of some pro or anti-inflammatory cytokines in colonic
tissue homogenate?
Discussion
- “In the current study application of AA disturbed the colonic mucus” How did you evaluate
that? Because there was no result on the amount of colonic mucus!
- In general, English editing is required.
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