Reviewer's report
Title:Prevalence of Helicobacter pylori high tetracycline resistant 16S rDNA
AGA926-928TTC genotype in gastric biopsy specimens from dyspeptic patients
of a city in the interior of Sao Paulo, Brazil
Version: 1Date:3 February 2012
Reviewer: Dawn Israel
Reviewer's report:
Antibiotic resistance among H. pylori stains is of clinical relevance and increasing
significance. Some studies have previously reported frequent tetracycline
resistance (TetR) in H. pylori isolates from countries including Brazil. Therefore,
in the study presented, the investigators’ goal was to assess the utility of
PCR/RFLP technique to predict TetR in clinical samples from Brazil. H. pylori
was diagnosed by histology, DNA was isolated from biopsies determined to be H.
pylori positive and these samples were subjected to PCR of a region of 16SrRNA
followed by RFLP analysis.
Major Compulsory Revisions
Unfortunately, no samples were cultured in order to determine definitively the
susceptibility to tetracycline. The investigators did not identify the mutation
expected to result in TetR in their samples, but since the resistance status of the
strains was not determined, it cannot be concluded that the technique is useful or
not since the result overall was a negative one. Further, the manuscript was
difficult to uncommon usage of English at times and numerous misspellings.
Minor Essential Revisions
1. P. 4. I do not understand the last sentence on this page continuing to page 5.
2. P. 6, 3rd paragraph. The “household rapid urease test” might be omitted if
usable results were not obtained. If it is included, it be renamed “rapid urease
test” and described in the methods.
3. P. 7, 2nd paragraph. “926, 927 e 928”: “e” should be “and”.
4. Results. If TetR H. pylori is highly prevalent in this population, further
possibilities should be described regarding the lack of the predicted mutation.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:Prevalence of Helicobacter pylori high tetracycline resistant 16S rDNA
AGA926-928TTC genotype in gastric biopsy specimens from dyspeptic patients
of a city in the interior of Sao Paulo, Brazil
Version: 1Date:3 February 2012
Reviewer: Dawn Israel
Reviewer's report:
Antibiotic resistance among H. pylori stains is of clinical relevance and increasing
significance. Some studies have previously reported frequent tetracycline
resistance (TetR) in H. pylori isolates from countries including Brazil. Therefore,
in the study presented, the investigators’ goal was to assess the utility of
PCR/RFLP technique to predict TetR in clinical samples from Brazil. H. pylori
was diagnosed by histology, DNA was isolated from biopsies determined to be H.
pylori positive and these samples were subjected to PCR of a region of 16SrRNA
followed by RFLP analysis.
Major Compulsory Revisions
Unfortunately, no samples were cultured in order to determine definitively the
susceptibility to tetracycline. The investigators did not identify the mutation
expected to result in TetR in their samples, but since the resistance status of the
strains was not determined, it cannot be concluded that the technique is useful or
not since the result overall was a negative one. Further, the manuscript was
difficult to uncommon usage of English at times and numerous misspellings.
Minor Essential Revisions
1. P. 4. I do not understand the last sentence on this page continuing to page 5.
2. P. 6, 3rd paragraph. The “household rapid urease test” might be omitted if
usable results were not obtained. If it is included, it be renamed “rapid urease
test” and described in the methods.
3. P. 7, 2nd paragraph. “926, 927 e 928”: “e” should be “and”.
4. Results. If TetR H. pylori is highly prevalent in this population, further
possibilities should be described regarding the lack of the predicted mutation.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:Prevalence of Helicobacter pylori high tetracycline resistant 16S rDNA
AGA926-928TTC genotype in gastric biopsy specimens from dyspeptic patients
of a city in the interior of Sao Paulo, Brazil
Version: 1Date:3 February 2012
Reviewer: Dawn Israel
Reviewer's report:
Antibiotic resistance among H. pylori stains is of clinical relevance and increasing
significance. Some studies have previously reported frequent tetracycline
resistance (TetR) in H. pylori isolates from countries including Brazil. Therefore,
in the study presented, the investigators’ goal was to assess the utility of
PCR/RFLP technique to predict TetR in clinical samples from Brazil. H. pylori
was diagnosed by histology, DNA was isolated from biopsies determined to be H.
pylori positive and these samples were subjected to PCR of a region of 16SrRNA
followed by RFLP analysis.
Major Compulsory Revisions
Unfortunately, no samples were cultured in order to determine definitively the
susceptibility to tetracycline. The investigators did not identify the mutation
expected to result in TetR in their samples, but since the resistance status of the
strains was not determined, it cannot be concluded that the technique is useful or
not since the result overall was a negative one. Further, the manuscript was
difficult to uncommon usage of English at times and numerous misspellings.
Minor Essential Revisions
1. P. 4. I do not understand the last sentence on this page continuing to page 5.
2. P. 6, 3rd paragraph. The “household rapid urease test” might be omitted if
usable results were not obtained. If it is included, it be renamed “rapid urease
test” and described in the methods.
3. P. 7, 2nd paragraph. “926, 927 e 928”: “e” should be “and”.
4. Results. If TetR H. pylori is highly prevalent in this population, further
possibilities should be described regarding the lack of the predicted mutation.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
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