Title: RNA N6-methyladenosine demethylase FTO targets MOXD1 promoting the malignant
phenotype of gastric cancer
Version: 2 Date: 12 Sep 2023
Reviewer's report:
In the current study the author explored the expression and clinical effect of FTO in gastric
cancer and further investigated the possible mechanisms. There are several points need to be
considered carefully
1. The expression status, survival analysis and function of FTO have been well studied in GC
in previous study (PMID 35064107) thus the significance of the current study is poor.
2. Many grammatical mistakes are found in the manuscript in the background section “Aims of
this study to analyze the function of differentially expressed gene of FTO depleted GC cells,
and further explore the targeted genes of FTO downstream modification in GC.”
3. All the expression data are present with public data set or qPCR. The protein should be
examined.
4. In Figure 7C the gating of the FACS should be identical in NC and MOXD1 knock down
gourp
4. The author explored the function of FTO and MOXD1 separately but the author intended to
show “FTO targets MOXD1 promoting the malignant phenotype of gastric cancer”. Since the
function of FTO in GC has been studied in GC, to increase the novelty of the current study the
author should investigate the function and mechanisms of MOXD1 instead of FTO
”
Title: RNA N6-methyladenosine demethylase FTO targets MOXD1 promoting the malignant
phenotype of gastric cancer
Version: 2 Date: 12 Sep 2023
Reviewer's report:
In the current study the author explored the expression and clinical effect of FTO in gastric
cancer and further investigated the possible mechanisms. There are several points need to be
considered carefully
1. The expression status, survival analysis and function of FTO have been well studied in GC
in previous study (PMID 35064107) thus the significance of the current study is poor.
2. Many grammatical mistakes are found in the manuscript in the background section “Aims of
this study to analyze the function of differentially expressed gene of FTO depleted GC cells,
and further explore the targeted genes of FTO downstream modification in GC.”
3. All the expression data are present with public data set or qPCR. The protein should be
examined.
4. In Figure 7C the gating of the FACS should be identical in NC and MOXD1 knock down
gourp
4. The author explored the function of FTO and MOXD1 separately but the author intended to
show “FTO targets MOXD1 promoting the malignant phenotype of gastric cancer”. Since the
function of FTO in GC has been studied in GC, to increase the novelty of the current study the
author should investigate the function and mechanisms of MOXD1 instead of FTO
”
Please review this paper.
user
Title: RNA N6-methyladenosine demethylase FTO targets MOXD1 promoting the malignant
phenotype of gastric cancer
Version: 2 Date: 12 Sep 2023
Reviewer's report:
In the current study the author explored the expression and clinical effect of FTO in gastric
cancer and further investigated the possible mechanisms. There are several points need to be
considered carefully
1. The expression status, survival analysis and function of FTO have been well studied in GC
in previous study (PMID 35064107) thus the significance of the current study is poor.
2. Many grammatical mistakes are found in the manuscript in the background section “Aims of
this study to analyze the function of differentially expressed gene of FTO depleted GC cells,
and further explore the targeted genes of FTO downstream modification in GC.”
3. All the expression data are present with public data set or qPCR. The protein should be
examined.
4. In Figure 7C the gating of the FACS should be identical in NC and MOXD1 knock down
gourp
4. The author explored the function of FTO and MOXD1 separately but the author intended to
show “FTO targets MOXD1 promoting the malignant phenotype of gastric cancer”. Since the
function of FTO in GC has been studied in GC, to increase the novelty of the current study the
author should investigate the function and mechanisms of MOXD1 instead of FTO
”
assistant