Title: Common laboratory blood test immune panel markers are useful for grading ulcerative
colitis endoscopic severity
Version: 1 Date: 04 Nov 2022
Reviewer's report:
Dear Editor, Authors
Thank you for sending manuscript entitled’ Common laboratory blood test immune panel
markers are useful for grading ulcerative colitis endoscopic severity: a case control study’ for
revision, kindly accept the following comments
- Frist, authors  ignore the presence of specific validated markers like calprotectin which actually
included in international guidelines
-  Authors mentioned it is a  retrospective study and in same time they mentioned in methodology
‘The blood samples were obtained on an empty stomach in the morning. Laboratory investigators
were blinded to the patient clinical data’ usually this is done in prospective studies.
- Also how can authors such details in retrospective study’  , visible blood in the stool (percentage
of movements), fecal incontinence, abdominal pain or cramping, general well-being, abdominal
tenderness, and a need for anti-diarrheal drugs.’
- Other systemic infections should be within exclusion criteria.
- Long sentences , frequent grammatical mistakes
- Finally, it is a good idea searching for inflammatory markers , but such markers included in
the study could be sensitive not specific . data mentioned in methodology should be sure trusted
as it obtained only from patient files, while authors gave specific details or instructions for lab
study like blind investigations done without background about patient information, HOW SUCH
DETAILS WRITTEN IN RETROSPECTIVE STUDY.
- Thanks
Title: Common laboratory blood test immune panel markers are useful for grading ulcerative
colitis endoscopic severity
Version: 1 Date: 04 Nov 2022
Reviewer's report:
Dear Editor, Authors
Thank you for sending manuscript entitled’ Common laboratory blood test immune panel
markers are useful for grading ulcerative colitis endoscopic severity: a case control study’ for
revision, kindly accept the following comments
- Frist, authors  ignore the presence of specific validated markers like calprotectin which actually
included in international guidelines
-  Authors mentioned it is a  retrospective study and in same time they mentioned in methodology
‘The blood samples were obtained on an empty stomach in the morning. Laboratory investigators
were blinded to the patient clinical data’ usually this is done in prospective studies.
- Also how can authors such details in retrospective study’  , visible blood in the stool (percentage
of movements), fecal incontinence, abdominal pain or cramping, general well-being, abdominal
tenderness, and a need for anti-diarrheal drugs.’
- Other systemic infections should be within exclusion criteria.
- Long sentences , frequent grammatical mistakes
- Finally, it is a good idea searching for inflammatory markers , but such markers included in
the study could be sensitive not specific . data mentioned in methodology should be sure trusted
as it obtained only from patient files, while authors gave specific details or instructions for lab
study like blind investigations done without background about patient information, HOW SUCH
DETAILS WRITTEN IN RETROSPECTIVE STUDY.
- Thanks
Please review this paper.
user
Title: Common laboratory blood test immune panel markers are useful for grading ulcerative
colitis endoscopic severity
Version: 1 Date: 04 Nov 2022
Reviewer's report:
Dear Editor, Authors
Thank you for sending manuscript entitled’ Common laboratory blood test immune panel
markers are useful for grading ulcerative colitis endoscopic severity: a case control study’ for
revision, kindly accept the following comments
- Frist, authors  ignore the presence of specific validated markers like calprotectin which actually
included in international guidelines
-  Authors mentioned it is a  retrospective study and in same time they mentioned in methodology
‘The blood samples were obtained on an empty stomach in the morning. Laboratory investigators
were blinded to the patient clinical data’ usually this is done in prospective studies.
- Also how can authors such details in retrospective study’  , visible blood in the stool (percentage
of movements), fecal incontinence, abdominal pain or cramping, general well-being, abdominal
tenderness, and a need for anti-diarrheal drugs.’
- Other systemic infections should be within exclusion criteria.
- Long sentences , frequent grammatical mistakes
- Finally, it is a good idea searching for inflammatory markers , but such markers included in
the study could be sensitive not specific . data mentioned in methodology should be sure trusted
as it obtained only from patient files, while authors gave specific details or instructions for lab
study like blind investigations done without background about patient information, HOW SUCH
DETAILS WRITTEN IN RETROSPECTIVE STUDY.
- Thanks
assistant