Title: PLAGL1 is associated with prognosis and cell proliferation in pancreatic adenocarcinoma
Version: 0 Date: 07 Nov 2022
Reviewer's report:
With interest, I read the paper by Liang et al., titled “Downregulated PLAGL1 is associated
with tumor progression and predicts poor prognosis in pancreatic adenocarcinoma”. This is
a good study confirming that downregulated PLAGL1 predicts poor prognosis in pancreatic
adenocarcinoma using several cohorts (TCGA, GEO, Renji Hospital, Shanghai OutdoBiotech
Ltd.). I have several comments.
Majors:
Based on current findings, the conclusion that downregulated PLAGL1 is associated with tumor
progression cannot be obtained. And “PLAGL1 inhibits the proliferation of PAAD by regulating
the cell cycle and DNA replication”should be revised. The impact of PLAGL1 overexpression
or silence on the function of pancreatic cancer cells, especially proliferation should be explored.
Minors:
1# PAAD synonym should be listed in Abstract.
2# Fig 1D IHC figure is blur.
3# Fig 2A, for stage III-IV pancreatic cancer, how to get specimens, by resection or FNA?
4# Fig 2B, what is NSUN6?
5# Fig 4A, please explain which portal was used to analyze the TCGA data. I checked the
expression of PLAGL1 in GEPIA and found the P value of 0.041, not P < 0.001 (http://
gepia2.cancer-pku.cn/#survival)
6# Fig 2C, it is confused to see the figure. Maybe better to present the result using table format.
7# “The relationship between PLAGL1 and MKI67 expression levels also confirmed that the
mRNA expression level of PLAGL1 was significantly negatively correlated with MKI67 mRNA
expression levels in the TCGA-PAAD cohort, GSE28735 cohort and our PAAD verification
cohort 1 (Fig. 2d-2f).” Delete “significantly”
8# The capacities of these PLAGL1 nearby genes somehow imply a the similar function of
PLAGL1. Correct “a the”
Title: PLAGL1 is associated with prognosis and cell proliferation in pancreatic adenocarcinoma
Version: 0 Date: 07 Nov 2022
Reviewer's report:
With interest, I read the paper by Liang et al., titled “Downregulated PLAGL1 is associated
with tumor progression and predicts poor prognosis in pancreatic adenocarcinoma”. This is
a good study confirming that downregulated PLAGL1 predicts poor prognosis in pancreatic
adenocarcinoma using several cohorts (TCGA, GEO, Renji Hospital, Shanghai OutdoBiotech
Ltd.). I have several comments.
Majors:
Based on current findings, the conclusion that downregulated PLAGL1 is associated with tumor
progression cannot be obtained. And “PLAGL1 inhibits the proliferation of PAAD by regulating
the cell cycle and DNA replication”should be revised. The impact of PLAGL1 overexpression
or silence on the function of pancreatic cancer cells, especially proliferation should be explored.
Minors:
1# PAAD synonym should be listed in Abstract.
2# Fig 1D IHC figure is blur.
3# Fig 2A, for stage III-IV pancreatic cancer, how to get specimens, by resection or FNA?
4# Fig 2B, what is NSUN6?
5# Fig 4A, please explain which portal was used to analyze the TCGA data. I checked the
expression of PLAGL1 in GEPIA and found the P value of 0.041, not P < 0.001 (http://
gepia2.cancer-pku.cn/#survival)
6# Fig 2C, it is confused to see the figure. Maybe better to present the result using table format.
7# “The relationship between PLAGL1 and MKI67 expression levels also confirmed that the
mRNA expression level of PLAGL1 was significantly negatively correlated with MKI67 mRNA
expression levels in the TCGA-PAAD cohort, GSE28735 cohort and our PAAD verification
cohort 1 (Fig. 2d-2f).” Delete “significantly”
8# The capacities of these PLAGL1 nearby genes somehow imply a the similar function of
PLAGL1. Correct “a the”
Please review this paper.
user
Title: PLAGL1 is associated with prognosis and cell proliferation in pancreatic adenocarcinoma
Version: 0 Date: 07 Nov 2022
Reviewer's report:
With interest, I read the paper by Liang et al., titled “Downregulated PLAGL1 is associated
with tumor progression and predicts poor prognosis in pancreatic adenocarcinoma”. This is
a good study confirming that downregulated PLAGL1 predicts poor prognosis in pancreatic
adenocarcinoma using several cohorts (TCGA, GEO, Renji Hospital, Shanghai OutdoBiotech
Ltd.). I have several comments.
Majors:
Based on current findings, the conclusion that downregulated PLAGL1 is associated with tumor
progression cannot be obtained. And “PLAGL1 inhibits the proliferation of PAAD by regulating
the cell cycle and DNA replication”should be revised. The impact of PLAGL1 overexpression
or silence on the function of pancreatic cancer cells, especially proliferation should be explored.
Minors:
1# PAAD synonym should be listed in Abstract.
2# Fig 1D IHC figure is blur.
3# Fig 2A, for stage III-IV pancreatic cancer, how to get specimens, by resection or FNA?
4# Fig 2B, what is NSUN6?
5# Fig 4A, please explain which portal was used to analyze the TCGA data. I checked the
expression of PLAGL1 in GEPIA and found the P value of 0.041, not P < 0.001 (http://
gepia2.cancer-pku.cn/#survival)
6# Fig 2C, it is confused to see the figure. Maybe better to present the result using table format.
7# “The relationship between PLAGL1 and MKI67 expression levels also confirmed that the
mRNA expression level of PLAGL1 was significantly negatively correlated with MKI67 mRNA
expression levels in the TCGA-PAAD cohort, GSE28735 cohort and our PAAD verification
cohort 1 (Fig. 2d-2f).” Delete “significantly”
8# The capacities of these PLAGL1 nearby genes somehow imply a the similar function of
PLAGL1. Correct “a the”
assistant