Reviewer's report
Title:Systemic administration of a novel human umbilical cord mesenchymal
stem cells population accelerate the resolution of acute liver injury
Version: 2Date:27 February 2012
Reviewer: Claus Kordes
Reviewer's report:
Major Compulsory Revision:
The authors improved their manuscript substantially. However, the TGF-beta
isoform measured by qPCR is still not mentioned in the manuscript. A detailed
analysis of the primer sequences of mice used by the authors revealed that a
target gene of TGF-beta 1 (i.e. transforming growth factor beta 1 induced
transcript 1 or Tgfb1i1; NM_009365.2), but not TGF-beta 1 was measured.
Moreover, the primers for IL-5 and beta-actin of mice are barely homologous to
the mRNA reference sequences NM_010558 and NM_007393. In addition, it is
surprising that the authors are not able to show the basal expression of albumin
and alpha-fetoprotein by RT-PCR in a normal agarose gel, which is also a very
sensitive method. If the “no template control” is not included, the qPCR can also
provide false positive data through measuring for example primer dimers. This
should be excluded.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:Systemic administration of a novel human umbilical cord mesenchymal
stem cells population accelerate the resolution of acute liver injury
Version: 2Date:27 February 2012
Reviewer: Claus Kordes
Reviewer's report:
Major Compulsory Revision:
The authors improved their manuscript substantially. However, the TGF-beta
isoform measured by qPCR is still not mentioned in the manuscript. A detailed
analysis of the primer sequences of mice used by the authors revealed that a
target gene of TGF-beta 1 (i.e. transforming growth factor beta 1 induced
transcript 1 or Tgfb1i1; NM_009365.2), but not TGF-beta 1 was measured.
Moreover, the primers for IL-5 and beta-actin of mice are barely homologous to
the mRNA reference sequences NM_010558 and NM_007393. In addition, it is
surprising that the authors are not able to show the basal expression of albumin
and alpha-fetoprotein by RT-PCR in a normal agarose gel, which is also a very
sensitive method. If the “no template control” is not included, the qPCR can also
provide false positive data through measuring for example primer dimers. This
should be excluded.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:Systemic administration of a novel human umbilical cord mesenchymal
stem cells population accelerate the resolution of acute liver injury
Version: 2Date:27 February 2012
Reviewer: Claus Kordes
Reviewer's report:
Major Compulsory Revision:
The authors improved their manuscript substantially. However, the TGF-beta
isoform measured by qPCR is still not mentioned in the manuscript. A detailed
analysis of the primer sequences of mice used by the authors revealed that a
target gene of TGF-beta 1 (i.e. transforming growth factor beta 1 induced
transcript 1 or Tgfb1i1; NM_009365.2), but not TGF-beta 1 was measured.
Moreover, the primers for IL-5 and beta-actin of mice are barely homologous to
the mRNA reference sequences NM_010558 and NM_007393. In addition, it is
surprising that the authors are not able to show the basal expression of albumin
and alpha-fetoprotein by RT-PCR in a normal agarose gel, which is also a very
sensitive method. If the “no template control” is not included, the qPCR can also
provide false positive data through measuring for example primer dimers. This
should be excluded.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
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