Reviewer's report
Title:The role of prostaglandin E2 (PGE2) in toll-like receptor 4 (TLR4)-mediated
colitis-associated neoplasia
Version: 1Date:24 February 2010
Reviewer: Jin-Kyung Kim
Reviewer's report:
The authors claim that their work demonstrated that exogenous PGE2
administration increase colitis-associated tumorigenesis via activation of EGFR
signaling pathway and dysregulation of PGE2 synthesis in TLR4 KO mice.
I have a few minor concerns that need to be addressed.
Fig. 2B,
Size-bar should be indicated.
Fig. 3C,
The levels of 15d-PGJ2 of colon in experimental groups were shown by
bar-graph. If the PGE2 levels were shown same pattern (not just explain in the
text), the audience easily understand that PGE2 administration affects the
balance between 15d-PGJ2 and PGE2.
Could the author explain the reason(s) how PGE2 administration increased the
level of 15d-PGJ2 in colon.
Fig. 4A,
Magnification and size-bar should be indicated.
Fig. 5A,
The author showed the mRNA levels of AR to explain PGE2 regulate
EGFR-signaling pathway in TLR4 KO mice. Based on their previous work (2007),
they showed the protein levels of AR. How about the protein levels in the present
study ?
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
Reviewer's report
Title:The role of prostaglandin E2 (PGE2) in toll-like receptor 4 (TLR4)-mediated
colitis-associated neoplasia
Version: 1Date:24 February 2010
Reviewer: Jin-Kyung Kim
Reviewer's report:
The authors claim that their work demonstrated that exogenous PGE2
administration increase colitis-associated tumorigenesis via activation of EGFR
signaling pathway and dysregulation of PGE2 synthesis in TLR4 KO mice.
I have a few minor concerns that need to be addressed.
Fig. 2B,
Size-bar should be indicated.
Fig. 3C,
The levels of 15d-PGJ2 of colon in experimental groups were shown by
bar-graph. If the PGE2 levels were shown same pattern (not just explain in the
text), the audience easily understand that PGE2 administration affects the
balance between 15d-PGJ2 and PGE2.
Could the author explain the reason(s) how PGE2 administration increased the
level of 15d-PGJ2 in colon.
Fig. 4A,
Magnification and size-bar should be indicated.
Fig. 5A,
The author showed the mRNA levels of AR to explain PGE2 regulate
EGFR-signaling pathway in TLR4 KO mice. Based on their previous work (2007),
they showed the protein levels of AR. How about the protein levels in the present
study ?
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
Please review this paper.
user
Reviewer's report
Title:The role of prostaglandin E2 (PGE2) in toll-like receptor 4 (TLR4)-mediated
colitis-associated neoplasia
Version: 1Date:24 February 2010
Reviewer: Jin-Kyung Kim
Reviewer's report:
The authors claim that their work demonstrated that exogenous PGE2
administration increase colitis-associated tumorigenesis via activation of EGFR
signaling pathway and dysregulation of PGE2 synthesis in TLR4 KO mice.
I have a few minor concerns that need to be addressed.
Fig. 2B,
Size-bar should be indicated.
Fig. 3C,
The levels of 15d-PGJ2 of colon in experimental groups were shown by
bar-graph. If the PGE2 levels were shown same pattern (not just explain in the
text), the audience easily understand that PGE2 administration affects the
balance between 15d-PGJ2 and PGE2.
Could the author explain the reason(s) how PGE2 administration increased the
level of 15d-PGJ2 in colon.
Fig. 4A,
Magnification and size-bar should be indicated.
Fig. 5A,
The author showed the mRNA levels of AR to explain PGE2 regulate
EGFR-signaling pathway in TLR4 KO mice. Based on their previous work (2007),
they showed the protein levels of AR. How about the protein levels in the present
study ?
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
assistant