Reviewer's report
Title:Induction of G1 and G2/M cell cycle arrests by the dietary compound
3,3'-diindolylmethane in HT-29 human colon cancer cells
Version: 1Date:2 February 2009
Reviewer: Farrukh Afaq
Reviewer's report:
In this study, the authors examined the effect of 3,3’-diindolylmetnane (DIM) on
cell cycle progression in HT-29 cells. This study is an extension of the previous
work in which the authors have shown that DIM inhibited the growth of colon
cells. Here, the authors further suggest that treatment of cells with DIM resulted
in a dose-dependent reduction in DNA synthesis. Furthermore, the percentage of
cells in G1 and G2/M phases were significantly increased after DIM treatment
and this was accompanied by reduced expression cyclins and cdk4 an increased
expression of p21 1nd p27. This is an interesting study and the experiments
appear to be well-planned and conducted. There are some concerns which are
listed below:
The authors should also provide dose-dependent data to further prove that DIM
treatment resulted in G1 and G2/M phase arrest.
The authors should also examine the effect of DIM on normal cells?
Since the expression of p21 is tightly regulated by p53, the authors are
encouraged to examine the status of p53 after DIM treatment.
What was the effect of DIM on p16 expression?
Some of the sentences in the manuscript are difficult to understand and need
rephrasing.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
'I declare that I have no competing interests'
Reviewer's report
Title:Induction of G1 and G2/M cell cycle arrests by the dietary compound
3,3'-diindolylmethane in HT-29 human colon cancer cells
Version: 1Date:2 February 2009
Reviewer: Farrukh Afaq
Reviewer's report:
In this study, the authors examined the effect of 3,3’-diindolylmetnane (DIM) on
cell cycle progression in HT-29 cells. This study is an extension of the previous
work in which the authors have shown that DIM inhibited the growth of colon
cells. Here, the authors further suggest that treatment of cells with DIM resulted
in a dose-dependent reduction in DNA synthesis. Furthermore, the percentage of
cells in G1 and G2/M phases were significantly increased after DIM treatment
and this was accompanied by reduced expression cyclins and cdk4 an increased
expression of p21 1nd p27. This is an interesting study and the experiments
appear to be well-planned and conducted. There are some concerns which are
listed below:
The authors should also provide dose-dependent data to further prove that DIM
treatment resulted in G1 and G2/M phase arrest.
The authors should also examine the effect of DIM on normal cells?
Since the expression of p21 is tightly regulated by p53, the authors are
encouraged to examine the status of p53 after DIM treatment.
What was the effect of DIM on p16 expression?
Some of the sentences in the manuscript are difficult to understand and need
rephrasing.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
'I declare that I have no competing interests'
Please review this paper.
user
Reviewer's report
Title:Induction of G1 and G2/M cell cycle arrests by the dietary compound
3,3'-diindolylmethane in HT-29 human colon cancer cells
Version: 1Date:2 February 2009
Reviewer: Farrukh Afaq
Reviewer's report:
In this study, the authors examined the effect of 3,3’-diindolylmetnane (DIM) on
cell cycle progression in HT-29 cells. This study is an extension of the previous
work in which the authors have shown that DIM inhibited the growth of colon
cells. Here, the authors further suggest that treatment of cells with DIM resulted
in a dose-dependent reduction in DNA synthesis. Furthermore, the percentage of
cells in G1 and G2/M phases were significantly increased after DIM treatment
and this was accompanied by reduced expression cyclins and cdk4 an increased
expression of p21 1nd p27. This is an interesting study and the experiments
appear to be well-planned and conducted. There are some concerns which are
listed below:
The authors should also provide dose-dependent data to further prove that DIM
treatment resulted in G1 and G2/M phase arrest.
The authors should also examine the effect of DIM on normal cells?
Since the expression of p21 is tightly regulated by p53, the authors are
encouraged to examine the status of p53 after DIM treatment.
What was the effect of DIM on p16 expression?
Some of the sentences in the manuscript are difficult to understand and need
rephrasing.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
'I declare that I have no competing interests'
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