Reviewer's report
Title:TNF-alpha and LPA promote synergistic expression of COX-2 in human
colonic myofibroblasts: role of LPA-mediated transactivation of upregulated
EGFR
Version: 1Date:4 October 2012
Reviewer: ling xu
Reviewer's report:
1.The author investigated whether EGFR signaling plays a role in the synergistic
COX-2 expression induced by LPA and TNF--#.18Co cells were treated with
TNF-##and LPA . EGFR and COX-2 protein expression, Y1068 phosphorylation,
and p42/44 MAPK phosphorylation were assessed by Western Blot. The results
were described by 5 steps. Design of this experiment is reasonable. Results
explained clearly.
2.The author has done pre-research “TNF-alpha induces upregulation of EGFR
expression and signaling in human colonic myofibroblasts. Am J Physiol
Gastrointest Liver Physiol, 302(8):G805-814.” This manuscript was expressed a
further study. The data and graph were explained clearly. The writing of
manuscript is acceptable.
3.The method of research is only done by Western Blot. It will be better that
adding the detection in the level of gene in this study.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:TNF-alpha and LPA promote synergistic expression of COX-2 in human
colonic myofibroblasts: role of LPA-mediated transactivation of upregulated
EGFR
Version: 1Date:4 October 2012
Reviewer: ling xu
Reviewer's report:
1.The author investigated whether EGFR signaling plays a role in the synergistic
COX-2 expression induced by LPA and TNF--#.18Co cells were treated with
TNF-##and LPA . EGFR and COX-2 protein expression, Y1068 phosphorylation,
and p42/44 MAPK phosphorylation were assessed by Western Blot. The results
were described by 5 steps. Design of this experiment is reasonable. Results
explained clearly.
2.The author has done pre-research “TNF-alpha induces upregulation of EGFR
expression and signaling in human colonic myofibroblasts. Am J Physiol
Gastrointest Liver Physiol, 302(8):G805-814.” This manuscript was expressed a
further study. The data and graph were explained clearly. The writing of
manuscript is acceptable.
3.The method of research is only done by Western Blot. It will be better that
adding the detection in the level of gene in this study.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:TNF-alpha and LPA promote synergistic expression of COX-2 in human
colonic myofibroblasts: role of LPA-mediated transactivation of upregulated
EGFR
Version: 1Date:4 October 2012
Reviewer: ling xu
Reviewer's report:
1.The author investigated whether EGFR signaling plays a role in the synergistic
COX-2 expression induced by LPA and TNF--#.18Co cells were treated with
TNF-##and LPA . EGFR and COX-2 protein expression, Y1068 phosphorylation,
and p42/44 MAPK phosphorylation were assessed by Western Blot. The results
were described by 5 steps. Design of this experiment is reasonable. Results
explained clearly.
2.The author has done pre-research “TNF-alpha induces upregulation of EGFR
expression and signaling in human colonic myofibroblasts. Am J Physiol
Gastrointest Liver Physiol, 302(8):G805-814.” This manuscript was expressed a
further study. The data and graph were explained clearly. The writing of
manuscript is acceptable.
3.The method of research is only done by Western Blot. It will be better that
adding the detection in the level of gene in this study.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
assistant