Reviewer's report
Title:Epigenetic mechanisms involved in differential MDR1 mRNA expression
between gastric and colon cancer cell lines and rationales for clinical
chemotherapy
Version: 1Date:29 July 2007
Reviewer: Jing-Yuan FANG
Reviewer's report:
General
-------------------------------------------------------------------------------
Major Compulsory Revisions (that the author must respond to before a decision
on publication can be reached)
Specific points:
1. Why does authors did not assay the methylation of the promoter or/and exon 1
using the bisulfite modification and sequencing ( mapping ) ? Because the result
from mapping contains all of data from the MSP and restriction endonuclease
digestion as well as direct sequencing for MSP products. The primers design
problem? Please explain the reason.
2.Please add some publications published from 2002~2007, in References.
-------------------------------------------------------------------------
Minor Essential Revisions (such as missing labels on figures, or the wrong use of
a term, which the author can be trusted to correct)
-------------------------------------------------------------------------------
Discretionary Revisions (which the author can choose to ignore)
What next?: Accept after discretionary revisions
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:Epigenetic mechanisms involved in differential MDR1 mRNA expression
between gastric and colon cancer cell lines and rationales for clinical
chemotherapy
Version: 1Date:29 July 2007
Reviewer: Jing-Yuan FANG
Reviewer's report:
General
-------------------------------------------------------------------------------
Major Compulsory Revisions (that the author must respond to before a decision
on publication can be reached)
Specific points:
1. Why does authors did not assay the methylation of the promoter or/and exon 1
using the bisulfite modification and sequencing ( mapping ) ? Because the result
from mapping contains all of data from the MSP and restriction endonuclease
digestion as well as direct sequencing for MSP products. The primers design
problem? Please explain the reason.
2.Please add some publications published from 2002~2007, in References.
-------------------------------------------------------------------------
Minor Essential Revisions (such as missing labels on figures, or the wrong use of
a term, which the author can be trusted to correct)
-------------------------------------------------------------------------------
Discretionary Revisions (which the author can choose to ignore)
What next?: Accept after discretionary revisions
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:Epigenetic mechanisms involved in differential MDR1 mRNA expression
between gastric and colon cancer cell lines and rationales for clinical
chemotherapy
Version: 1Date:29 July 2007
Reviewer: Jing-Yuan FANG
Reviewer's report:
General
-------------------------------------------------------------------------------
Major Compulsory Revisions (that the author must respond to before a decision
on publication can be reached)
Specific points:
1. Why does authors did not assay the methylation of the promoter or/and exon 1
using the bisulfite modification and sequencing ( mapping ) ? Because the result
from mapping contains all of data from the MSP and restriction endonuclease
digestion as well as direct sequencing for MSP products. The primers design
problem? Please explain the reason.
2.Please add some publications published from 2002~2007, in References.
-------------------------------------------------------------------------
Minor Essential Revisions (such as missing labels on figures, or the wrong use of
a term, which the author can be trusted to correct)
-------------------------------------------------------------------------------
Discretionary Revisions (which the author can choose to ignore)
What next?: Accept after discretionary revisions
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
assistant