Title: The Effects of Low-Dose IL-2 on Th17/Treg Cell Imbalance in Primary Biliary
Cholangitis Mouse Models
Version: 3 Date: 13 Dec 2023
Reviewer's report:
The whole article should be rearranged for better understanding and to make it easier to follow
the researcher's thoughts. Moreover, the description of the method used in this study needs to
be written better and more consistently. Please improve this section for better transparency of
your results. Furthermore, the Authors should also improve the figure descriptions (legends).
Whether the KEGG analysis was performed on publicly available data (GSE79850 ) or whether
the authors performed those RNA-Seq needs to be clarified. Please indicate this information in
the main text.
The description of the results from bioinformatical analysis needs to be improved. Also, the
authors should consider rewriting the article title to make it more consistent with the conclusions
drawn.
The discussion is too general and requires specific improvement. Please distinguish which of the
cited studies concern in vitro analyses and which concern animal models or studies on human
material.
It should also be indicated which information in the literature is consistent and which is contrary
to the obtained results. Also, the discussion section needs to include the part that discusses the
limitations of this study.
The article contains minor language errors. English editing is suggested.
Details:
Line 113: please add the catalog number and manufacturer of the used antibodies
IHC method - please indicate how the staining was visualized. (DAB?)
Line 122: How were the results of the ELISA test analyzed after measurements?
Line 127: Please add the used software version.
Lines 222-223: This sentence should be removed from the discussion.
Title: The Effects of Low-Dose IL-2 on Th17/Treg Cell Imbalance in Primary Biliary
Cholangitis Mouse Models
Version: 3 Date: 13 Dec 2023
Reviewer's report:
The whole article should be rearranged for better understanding and to make it easier to follow
the researcher's thoughts. Moreover, the description of the method used in this study needs to
be written better and more consistently. Please improve this section for better transparency of
your results. Furthermore, the Authors should also improve the figure descriptions (legends).
Whether the KEGG analysis was performed on publicly available data (GSE79850 ) or whether
the authors performed those RNA-Seq needs to be clarified. Please indicate this information in
the main text.
The description of the results from bioinformatical analysis needs to be improved. Also, the
authors should consider rewriting the article title to make it more consistent with the conclusions
drawn.
The discussion is too general and requires specific improvement. Please distinguish which of the
cited studies concern in vitro analyses and which concern animal models or studies on human
material.
It should also be indicated which information in the literature is consistent and which is contrary
to the obtained results. Also, the discussion section needs to include the part that discusses the
limitations of this study.
The article contains minor language errors. English editing is suggested.
Details:
Line 113: please add the catalog number and manufacturer of the used antibodies
IHC method - please indicate how the staining was visualized. (DAB?)
Line 122: How were the results of the ELISA test analyzed after measurements?
Line 127: Please add the used software version.
Lines 222-223: This sentence should be removed from the discussion.
Please review this paper.
user
Title: The Effects of Low-Dose IL-2 on Th17/Treg Cell Imbalance in Primary Biliary
Cholangitis Mouse Models
Version: 3 Date: 13 Dec 2023
Reviewer's report:
The whole article should be rearranged for better understanding and to make it easier to follow
the researcher's thoughts. Moreover, the description of the method used in this study needs to
be written better and more consistently. Please improve this section for better transparency of
your results. Furthermore, the Authors should also improve the figure descriptions (legends).
Whether the KEGG analysis was performed on publicly available data (GSE79850 ) or whether
the authors performed those RNA-Seq needs to be clarified. Please indicate this information in
the main text.
The description of the results from bioinformatical analysis needs to be improved. Also, the
authors should consider rewriting the article title to make it more consistent with the conclusions
drawn.
The discussion is too general and requires specific improvement. Please distinguish which of the
cited studies concern in vitro analyses and which concern animal models or studies on human
material.
It should also be indicated which information in the literature is consistent and which is contrary
to the obtained results. Also, the discussion section needs to include the part that discusses the
limitations of this study.
The article contains minor language errors. English editing is suggested.
Details:
Line 113: please add the catalog number and manufacturer of the used antibodies
IHC method - please indicate how the staining was visualized. (DAB?)
Line 122: How were the results of the ELISA test analyzed after measurements?
Line 127: Please add the used software version.
Lines 222-223: This sentence should be removed from the discussion.
assistant