Reviewer's report
Title:Genetic deletion of dectin-1 does not affect the course of murine
experimental colitis.
Version: 2Date:2 February 2012
Reviewer: Colin de Haar
Reviewer's report:
The authors have answered all major points to my satisfaction and changed the
manuscript accordingly.
Although I do not fully agree with their comment with regard to point 7, I agree
with them that this will not be contribute to the conclusion they draw with regard
to dectin-1 in mouse colitis models, but instead will give broader information
about the role of fungi in colitis models.
Discretionary revision:
Point 8:
With regard to their response:
Blocking the IL-10R does affect the levels of IL-10 by blocking its consumption,
but more importantly it inhibit its function. Since IL-10 has recently been shown to
be the key cytokine that enables rescue from Hh-colits, it could still be interesting
to see if dectin-1 deficient mice will develop Hh-colits whereas the WT will not.
However, the limited difference in IL-10 levels between the WT and dectin-1
deficient macrophages would be a sensible reason not to perform this
experiment.
Minor essential revision:
Point 8: The involvement of the adaptive immune response is mentioned here as
rationale for the Hh-colitis model, but no data are presented with regard to the
adaptive immune response. Since IFN-gamma levels were measured in the
colon homogenates (M&M), these data could be included to show that at least
this “adaptive parameter” was not affected.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Genetic deletion of dectin-1 does not affect the course of murine
experimental colitis.
Version: 2Date:2 February 2012
Reviewer: Colin de Haar
Reviewer's report:
The authors have answered all major points to my satisfaction and changed the
manuscript accordingly.
Although I do not fully agree with their comment with regard to point 7, I agree
with them that this will not be contribute to the conclusion they draw with regard
to dectin-1 in mouse colitis models, but instead will give broader information
about the role of fungi in colitis models.
Discretionary revision:
Point 8:
With regard to their response:
Blocking the IL-10R does affect the levels of IL-10 by blocking its consumption,
but more importantly it inhibit its function. Since IL-10 has recently been shown to
be the key cytokine that enables rescue from Hh-colits, it could still be interesting
to see if dectin-1 deficient mice will develop Hh-colits whereas the WT will not.
However, the limited difference in IL-10 levels between the WT and dectin-1
deficient macrophages would be a sensible reason not to perform this
experiment.
Minor essential revision:
Point 8: The involvement of the adaptive immune response is mentioned here as
rationale for the Hh-colitis model, but no data are presented with regard to the
adaptive immune response. Since IFN-gamma levels were measured in the
colon homogenates (M&M), these data could be included to show that at least
this “adaptive parameter” was not affected.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Genetic deletion of dectin-1 does not affect the course of murine
experimental colitis.
Version: 2Date:2 February 2012
Reviewer: Colin de Haar
Reviewer's report:
The authors have answered all major points to my satisfaction and changed the
manuscript accordingly.
Although I do not fully agree with their comment with regard to point 7, I agree
with them that this will not be contribute to the conclusion they draw with regard
to dectin-1 in mouse colitis models, but instead will give broader information
about the role of fungi in colitis models.
Discretionary revision:
Point 8:
With regard to their response:
Blocking the IL-10R does affect the levels of IL-10 by blocking its consumption,
but more importantly it inhibit its function. Since IL-10 has recently been shown to
be the key cytokine that enables rescue from Hh-colits, it could still be interesting
to see if dectin-1 deficient mice will develop Hh-colits whereas the WT will not.
However, the limited difference in IL-10 levels between the WT and dectin-1
deficient macrophages would be a sensible reason not to perform this
experiment.
Minor essential revision:
Point 8: The involvement of the adaptive immune response is mentioned here as
rationale for the Hh-colitis model, but no data are presented with regard to the
adaptive immune response. Since IFN-gamma levels were measured in the
colon homogenates (M&M), these data could be included to show that at least
this “adaptive parameter” was not affected.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
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