Title: Macrophage polarization toward M1 phenotype in T cell transfer colitis model
Version: 1 Date: 23 Oct 2023
Reviewer:  Hui HAN
Reviewer's report:
In their manuscript, Ebihara and colleagues conducted research to investigate the role of
macrophages (MFs) in a preclinical model of colitis. The experimental design of the animal
model appears well-reasoned, incorporating a comprehensive range of parameters, including
colonoscopy, histology, the measurement of pro-inflammatory cytokines, and flow cytometry.
However, it is essential to note that this animal model has been extensively characterized in
previous studies. Overall, the manuscript is well-written and exhibits minimal errors.
Here are some comments aimed at enhancing the manuscript:
1. Providing an introductory section that outlines the specific role of MFs in colitis development
and discusses the challenges and treatment options for colitis by targeting MFs would greatly
benefit the reader's understanding.
2. What are the reasons behind the increase in M1 MFs in this animal model. Are there any
potential interactions between transferred T cells and MFs?
3. Whether the changes in MF markers observed in this model are replicable in human colitis.
This can be explored by comparing the findings to publicly available datasets.
4. It is unclear the rationale behind using dimension reduction techniques for the flow cytometry
data analysis. If the authors are primarily focused on one or two M1 and M2 markers, it may
be unnecessary.
5. In the context of the in vitro experiment, whether there are any other T cell activation markers
up regulated. It would be helpful if authors could clarify the T cell subsets they differentiated
into.
6. Are there any explanations on decreasing in M2 MFs? Is it possible that some M2 MFs
transition into the M1 phenotype during the progression of the disease?
7. In the discussion section, there's no need to reference specific figure numbers.
Title: Macrophage polarization toward M1 phenotype in T cell transfer colitis model
Version: 1 Date: 23 Oct 2023
Reviewer:  Hui HAN
Reviewer's report:
In their manuscript, Ebihara and colleagues conducted research to investigate the role of
macrophages (MFs) in a preclinical model of colitis. The experimental design of the animal
model appears well-reasoned, incorporating a comprehensive range of parameters, including
colonoscopy, histology, the measurement of pro-inflammatory cytokines, and flow cytometry.
However, it is essential to note that this animal model has been extensively characterized in
previous studies. Overall, the manuscript is well-written and exhibits minimal errors.
Here are some comments aimed at enhancing the manuscript:
1. Providing an introductory section that outlines the specific role of MFs in colitis development
and discusses the challenges and treatment options for colitis by targeting MFs would greatly
benefit the reader's understanding.
2. What are the reasons behind the increase in M1 MFs in this animal model. Are there any
potential interactions between transferred T cells and MFs?
3. Whether the changes in MF markers observed in this model are replicable in human colitis.
This can be explored by comparing the findings to publicly available datasets.
4. It is unclear the rationale behind using dimension reduction techniques for the flow cytometry
data analysis. If the authors are primarily focused on one or two M1 and M2 markers, it may
be unnecessary.
5. In the context of the in vitro experiment, whether there are any other T cell activation markers
up regulated. It would be helpful if authors could clarify the T cell subsets they differentiated
into.
6. Are there any explanations on decreasing in M2 MFs? Is it possible that some M2 MFs
transition into the M1 phenotype during the progression of the disease?
7. In the discussion section, there's no need to reference specific figure numbers.
Please review this paper.
user
Title: Macrophage polarization toward M1 phenotype in T cell transfer colitis model
Version: 1 Date: 23 Oct 2023
Reviewer:  Hui HAN
Reviewer's report:
In their manuscript, Ebihara and colleagues conducted research to investigate the role of
macrophages (MFs) in a preclinical model of colitis. The experimental design of the animal
model appears well-reasoned, incorporating a comprehensive range of parameters, including
colonoscopy, histology, the measurement of pro-inflammatory cytokines, and flow cytometry.
However, it is essential to note that this animal model has been extensively characterized in
previous studies. Overall, the manuscript is well-written and exhibits minimal errors.
Here are some comments aimed at enhancing the manuscript:
1. Providing an introductory section that outlines the specific role of MFs in colitis development
and discusses the challenges and treatment options for colitis by targeting MFs would greatly
benefit the reader's understanding.
2. What are the reasons behind the increase in M1 MFs in this animal model. Are there any
potential interactions between transferred T cells and MFs?
3. Whether the changes in MF markers observed in this model are replicable in human colitis.
This can be explored by comparing the findings to publicly available datasets.
4. It is unclear the rationale behind using dimension reduction techniques for the flow cytometry
data analysis. If the authors are primarily focused on one or two M1 and M2 markers, it may
be unnecessary.
5. In the context of the in vitro experiment, whether there are any other T cell activation markers
up regulated. It would be helpful if authors could clarify the T cell subsets they differentiated
into.
6. Are there any explanations on decreasing in M2 MFs? Is it possible that some M2 MFs
transition into the M1 phenotype during the progression of the disease?
7. In the discussion section, there's no need to reference specific figure numbers.
assistant