Title: A novel role for Helicobacter pylori cytotoxin-associated gene A in negative regulation
of autophagy in human gastric cells.
Version: 1 Date: 29 Mar 2023
Reviewer's report:
This original article found that CagA negatively regulates autophagy and promotes the
production of inflammatory cytokines by down-regulating BECN1. However, there were some
limitations in the experiment design, there are some questions that need to be answered.
1.The description of statistical methods is not clear. What software is used for statistical analysis?
2. Figure 4A The fourth group added autophagy inhibitor and enhancer, Figure 4B the fourth
group added autophagy inhibitor twice, which will affect the cell state, the significance of this
grouping?
3.This study was only verified at the cell level, but it was not verified in clinical samples.
4. The results of ATG5, ULK1 and P62 were not analyzed.
Title: A novel role for Helicobacter pylori cytotoxin-associated gene A in negative regulation
of autophagy in human gastric cells.
Version: 1 Date: 29 Mar 2023
Reviewer's report:
This original article found that CagA negatively regulates autophagy and promotes the
production of inflammatory cytokines by down-regulating BECN1. However, there were some
limitations in the experiment design, there are some questions that need to be answered.
1.The description of statistical methods is not clear. What software is used for statistical analysis?
2. Figure 4A The fourth group added autophagy inhibitor and enhancer, Figure 4B the fourth
group added autophagy inhibitor twice, which will affect the cell state, the significance of this
grouping?
3.This study was only verified at the cell level, but it was not verified in clinical samples.
4. The results of ATG5, ULK1 and P62 were not analyzed.
Please review this paper.
user
Title: A novel role for Helicobacter pylori cytotoxin-associated gene A in negative regulation
of autophagy in human gastric cells.
Version: 1 Date: 29 Mar 2023
Reviewer's report:
This original article found that CagA negatively regulates autophagy and promotes the
production of inflammatory cytokines by down-regulating BECN1. However, there were some
limitations in the experiment design, there are some questions that need to be answered.
1.The description of statistical methods is not clear. What software is used for statistical analysis?
2. Figure 4A The fourth group added autophagy inhibitor and enhancer, Figure 4B the fourth
group added autophagy inhibitor twice, which will affect the cell state, the significance of this
grouping?
3.This study was only verified at the cell level, but it was not verified in clinical samples.
4. The results of ATG5, ULK1 and P62 were not analyzed.
assistant