Title: LINC00634 is a novel prognostic biomarker for colon cancer associated with immune
infiltration
Version: 2 Date: 30 Dec 2023
Reviewer:  Giovanni Tomasicchio
Reviewer's report:
In this interesting study, the author used bioinformatics methods to screen genes related to
colon cancer, by constructing a predictive model, conducting in vitro experiments, and analyzing
its relationship with TIME. They showed a novel prognostic biomarker for colon cancer,
LINC00634. However, the author needs to improve the methods and results sections of the
paper. I suggest the authors follow the STROBE guidelines before writing a paper.
I have several questions for the author:
- In your introduction, you explain the role of TIME and lncRNAs in the progression of cancers,
but you didn’t explain their relationship. This is a key point for the aim of your study that you
wrote only at the end of the discussion. Please add, with references, in your introduction.
- Material and Methods is a crucial part of the paper, to understand results and discussion. You
need to modify your material and methods: you divided it in different paragraphs, writing parts
of the methods in the results, omitting information, and becoming unclear to the reader.
For example, you made a Kaplan-Meier analysis but didn’t write it in methods but directly in
results. Please write a single paragraph following the STROBE statement.
- Split the methods from the statistical part, you don’t need to write it for the different variables.
- Please add your study design in material and methods, you didn’t write it clearly in the text.
- Also, in the Results section, you made a mix of methods and discussion. You need to write only
your result you should not suggest your idea or discuss it in results. For example: “suggesting
that this gene set has potential ability to predict the prognosis of patients with colon cancer” or
“These results suggest that LINC00634” or “mediates the alteration of the immune environment
in colon cancer aroused our curiosity”
Title: LINC00634 is a novel prognostic biomarker for colon cancer associated with immune
infiltration
Version: 2 Date: 30 Dec 2023
Reviewer:  Giovanni Tomasicchio
Reviewer's report:
In this interesting study, the author used bioinformatics methods to screen genes related to
colon cancer, by constructing a predictive model, conducting in vitro experiments, and analyzing
its relationship with TIME. They showed a novel prognostic biomarker for colon cancer,
LINC00634. However, the author needs to improve the methods and results sections of the
paper. I suggest the authors follow the STROBE guidelines before writing a paper.
I have several questions for the author:
- In your introduction, you explain the role of TIME and lncRNAs in the progression of cancers,
but you didn’t explain their relationship. This is a key point for the aim of your study that you
wrote only at the end of the discussion. Please add, with references, in your introduction.
- Material and Methods is a crucial part of the paper, to understand results and discussion. You
need to modify your material and methods: you divided it in different paragraphs, writing parts
of the methods in the results, omitting information, and becoming unclear to the reader.
For example, you made a Kaplan-Meier analysis but didn’t write it in methods but directly in
results. Please write a single paragraph following the STROBE statement.
- Split the methods from the statistical part, you don’t need to write it for the different variables.
- Please add your study design in material and methods, you didn’t write it clearly in the text.
- Also, in the Results section, you made a mix of methods and discussion. You need to write only
your result you should not suggest your idea or discuss it in results. For example: “suggesting
that this gene set has potential ability to predict the prognosis of patients with colon cancer” or
“These results suggest that LINC00634” or “mediates the alteration of the immune environment
in colon cancer aroused our curiosity”
Please review this paper.
user
Title: LINC00634 is a novel prognostic biomarker for colon cancer associated with immune
infiltration
Version: 2 Date: 30 Dec 2023
Reviewer:  Giovanni Tomasicchio
Reviewer's report:
In this interesting study, the author used bioinformatics methods to screen genes related to
colon cancer, by constructing a predictive model, conducting in vitro experiments, and analyzing
its relationship with TIME. They showed a novel prognostic biomarker for colon cancer,
LINC00634. However, the author needs to improve the methods and results sections of the
paper. I suggest the authors follow the STROBE guidelines before writing a paper.
I have several questions for the author:
- In your introduction, you explain the role of TIME and lncRNAs in the progression of cancers,
but you didn’t explain their relationship. This is a key point for the aim of your study that you
wrote only at the end of the discussion. Please add, with references, in your introduction.
- Material and Methods is a crucial part of the paper, to understand results and discussion. You
need to modify your material and methods: you divided it in different paragraphs, writing parts
of the methods in the results, omitting information, and becoming unclear to the reader.
For example, you made a Kaplan-Meier analysis but didn’t write it in methods but directly in
results. Please write a single paragraph following the STROBE statement.
- Split the methods from the statistical part, you don’t need to write it for the different variables.
- Please add your study design in material and methods, you didn’t write it clearly in the text.
- Also, in the Results section, you made a mix of methods and discussion. You need to write only
your result you should not suggest your idea or discuss it in results. For example: “suggesting
that this gene set has potential ability to predict the prognosis of patients with colon cancer” or
“These results suggest that LINC00634” or “mediates the alteration of the immune environment
in colon cancer aroused our curiosity”
assistant