Title: OncomiR-181a promotes carcinogenesis by repressing the extracellular matrix
proteoglycan decorin in hepatocellular carcinoma
Version: 2 Date: 11 Aug 2024
Reviewer's report:
Several studies have found that decreased decorin (DCN) expression in HCC and its deficiency
promotes hepatic carcinogenesis (Front Oncol. 2020 May 12;10:645; Cell Signal. 2019
Oct;62:109354; Matrix Biol. 2014 Apr;35:194-205.). In this study, the authors discovered that
miR-181a targeted DCN at the 3’UTR and decreased the expression of decorin. miR-181a also
promoted cell viability, proliferation, and clonogenicity in HuH-7 cells. They also found that
miR-181a increased Myc and E2F while decreasing p53 and Rb signaling. However, there are
still some issues that need to be addressed.
1. Figure 1a shows mRNA levels of DCN in liver cancer, healthy liver tissues, and Huh7 cells.
What about the levels of miR-181a? The authors can supplement the results.
2. Figure 4c shows only a statistical graph for clonogenicity. Please display the figure under a
microscope.
3. The authors stated in their title that miR-181a promotes cell cycle progression. However,
I cannot see the direct proof in Figure 5. Please include an experiment to show that, such as
detecting with flow cytometry.
Title: OncomiR-181a promotes carcinogenesis by repressing the extracellular matrix
proteoglycan decorin in hepatocellular carcinoma
Version: 2 Date: 11 Aug 2024
Reviewer's report:
Several studies have found that decreased decorin (DCN) expression in HCC and its deficiency
promotes hepatic carcinogenesis (Front Oncol. 2020 May 12;10:645; Cell Signal. 2019
Oct;62:109354; Matrix Biol. 2014 Apr;35:194-205.). In this study, the authors discovered that
miR-181a targeted DCN at the 3’UTR and decreased the expression of decorin. miR-181a also
promoted cell viability, proliferation, and clonogenicity in HuH-7 cells. They also found that
miR-181a increased Myc and E2F while decreasing p53 and Rb signaling. However, there are
still some issues that need to be addressed.
1. Figure 1a shows mRNA levels of DCN in liver cancer, healthy liver tissues, and Huh7 cells.
What about the levels of miR-181a? The authors can supplement the results.
2. Figure 4c shows only a statistical graph for clonogenicity. Please display the figure under a
microscope.
3. The authors stated in their title that miR-181a promotes cell cycle progression. However,
I cannot see the direct proof in Figure 5. Please include an experiment to show that, such as
detecting with flow cytometry.
Please review this paper.
user
Title: OncomiR-181a promotes carcinogenesis by repressing the extracellular matrix
proteoglycan decorin in hepatocellular carcinoma
Version: 2 Date: 11 Aug 2024
Reviewer's report:
Several studies have found that decreased decorin (DCN) expression in HCC and its deficiency
promotes hepatic carcinogenesis (Front Oncol. 2020 May 12;10:645; Cell Signal. 2019
Oct;62:109354; Matrix Biol. 2014 Apr;35:194-205.). In this study, the authors discovered that
miR-181a targeted DCN at the 3’UTR and decreased the expression of decorin. miR-181a also
promoted cell viability, proliferation, and clonogenicity in HuH-7 cells. They also found that
miR-181a increased Myc and E2F while decreasing p53 and Rb signaling. However, there are
still some issues that need to be addressed.
1. Figure 1a shows mRNA levels of DCN in liver cancer, healthy liver tissues, and Huh7 cells.
What about the levels of miR-181a? The authors can supplement the results.
2. Figure 4c shows only a statistical graph for clonogenicity. Please display the figure under a
microscope.
3. The authors stated in their title that miR-181a promotes cell cycle progression. However,
I cannot see the direct proof in Figure 5. Please include an experiment to show that, such as
detecting with flow cytometry.
assistant