Title: Fecal biomarkers of environmental enteric dysfunction and associated factors among
children aged 24-59 months in east Dembiya district, northwest Ethiopia
Version: 0 Date: 18 Dec 2021
Reviewer's report:
Comments to author
In the Abstract section, the authors need to avoid abbreviation
Background
It is better to mention the global epidemiology of EED so as to show the gap of the study.
Methods
EED may be caused by parasites, bacteria, virus or fungi. However, the authors have mainly
focused on parasites.  I do not understand why the author did not isolate other microorganism
from the children’s stool rather than from food or water.
Line 100 : The author selected 238 out of 372 children, is 372 children the total population?
make it clear.
Line 108: Stool samples were collected without fixative for ELISA test. What about samples
used for intestinal parasite detection, with fixative or without fixative? If with with fixative,
what chemical was used?
Besides ELISA test procedures, A laboratory procedures for intestinal parasites and E. coli
detection should be mentioned in detail.
Result
line 160:  which parasites were predominantly identified ? B/se mentioning which parasites are
associated with fecal biomarker is something attractive to readers
Table 4 What is the purpose of analyzing EED b/se EED is measured by the three (AAT, MPO,
and NEO) . It is also better to say Adjusted odds ratio instead of adjusted beta
Title: Fecal biomarkers of environmental enteric dysfunction and associated factors among
children aged 24-59 months in east Dembiya district, northwest Ethiopia
Version: 0 Date: 18 Dec 2021
Reviewer's report:
Comments to author
In the Abstract section, the authors need to avoid abbreviation
Background
It is better to mention the global epidemiology of EED so as to show the gap of the study.
Methods
EED may be caused by parasites, bacteria, virus or fungi. However, the authors have mainly
focused on parasites.  I do not understand why the author did not isolate other microorganism
from the children’s stool rather than from food or water.
Line 100 : The author selected 238 out of 372 children, is 372 children the total population?
make it clear.
Line 108: Stool samples were collected without fixative for ELISA test. What about samples
used for intestinal parasite detection, with fixative or without fixative? If with with fixative,
what chemical was used?
Besides ELISA test procedures, A laboratory procedures for intestinal parasites and E. coli
detection should be mentioned in detail.
Result
line 160:  which parasites were predominantly identified ? B/se mentioning which parasites are
associated with fecal biomarker is something attractive to readers
Table 4 What is the purpose of analyzing EED b/se EED is measured by the three (AAT, MPO,
and NEO) . It is also better to say Adjusted odds ratio instead of adjusted beta
Please review this paper.
user
Title: Fecal biomarkers of environmental enteric dysfunction and associated factors among
children aged 24-59 months in east Dembiya district, northwest Ethiopia
Version: 0 Date: 18 Dec 2021
Reviewer's report:
Comments to author
In the Abstract section, the authors need to avoid abbreviation
Background
It is better to mention the global epidemiology of EED so as to show the gap of the study.
Methods
EED may be caused by parasites, bacteria, virus or fungi. However, the authors have mainly
focused on parasites.  I do not understand why the author did not isolate other microorganism
from the children’s stool rather than from food or water.
Line 100 : The author selected 238 out of 372 children, is 372 children the total population?
make it clear.
Line 108: Stool samples were collected without fixative for ELISA test. What about samples
used for intestinal parasite detection, with fixative or without fixative? If with with fixative,
what chemical was used?
Besides ELISA test procedures, A laboratory procedures for intestinal parasites and E. coli
detection should be mentioned in detail.
Result
line 160:  which parasites were predominantly identified ? B/se mentioning which parasites are
associated with fecal biomarker is something attractive to readers
Table 4 What is the purpose of analyzing EED b/se EED is measured by the three (AAT, MPO,
and NEO) . It is also better to say Adjusted odds ratio instead of adjusted beta
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