Title: CD10 and Das1: a biomarker study using immunohistochemistry to subtype gastric
intestinal metaplasia
Version: 0 Date: 06 Oct 2021
Reviewer's report:
This is an interesting topic and I think the study was well performed overall. However, there are
several major issues to be addressed.
1. There are already several studies investigating CD10 and DAS-1 as IM markers. If this study
was to validate the usefulness in clinical application, a larger number of samples should have
been included.
2. This study was aimed to explore the potential of CD10 and Das-1 as biomarkers to subtype
IM. I’m afraid it seems inappropriate to include gene expression analysis of IM in the results.
I suggest authors move it to supplementary data.
3. Authors determined the IM subtype only by histological evaluation. However, it can be hard
even for experienced pathologists because IM often occurs in mixed type or in hybrid type. Thus,
I believe that the determination of IM subtype should be backed up by ancillary studies such as
special stainings or other established IHC markers.
4. Hybrid-type IM is likely to express both CD10 and Das-1, which could confound the results.
I’m wondering whether there were no samples containing hybrid type IM.
5. In the lower panel of fig. 2B, authors claim that it is incomplete type IM, however, it seems
to be mixed type IM and Das-1 positive area is mostly complete IM, whereas incomplete type
IM portion is negative for Das-1.
Title: CD10 and Das1: a biomarker study using immunohistochemistry to subtype gastric
intestinal metaplasia
Version: 0 Date: 06 Oct 2021
Reviewer's report:
This is an interesting topic and I think the study was well performed overall. However, there are
several major issues to be addressed.
1. There are already several studies investigating CD10 and DAS-1 as IM markers. If this study
was to validate the usefulness in clinical application, a larger number of samples should have
been included.
2. This study was aimed to explore the potential of CD10 and Das-1 as biomarkers to subtype
IM. I’m afraid it seems inappropriate to include gene expression analysis of IM in the results.
I suggest authors move it to supplementary data.
3. Authors determined the IM subtype only by histological evaluation. However, it can be hard
even for experienced pathologists because IM often occurs in mixed type or in hybrid type. Thus,
I believe that the determination of IM subtype should be backed up by ancillary studies such as
special stainings or other established IHC markers.
4. Hybrid-type IM is likely to express both CD10 and Das-1, which could confound the results.
I’m wondering whether there were no samples containing hybrid type IM.
5. In the lower panel of fig. 2B, authors claim that it is incomplete type IM, however, it seems
to be mixed type IM and Das-1 positive area is mostly complete IM, whereas incomplete type
IM portion is negative for Das-1.
Please review this paper.
user
Title: CD10 and Das1: a biomarker study using immunohistochemistry to subtype gastric
intestinal metaplasia
Version: 0 Date: 06 Oct 2021
Reviewer's report:
This is an interesting topic and I think the study was well performed overall. However, there are
several major issues to be addressed.
1. There are already several studies investigating CD10 and DAS-1 as IM markers. If this study
was to validate the usefulness in clinical application, a larger number of samples should have
been included.
2. This study was aimed to explore the potential of CD10 and Das-1 as biomarkers to subtype
IM. I’m afraid it seems inappropriate to include gene expression analysis of IM in the results.
I suggest authors move it to supplementary data.
3. Authors determined the IM subtype only by histological evaluation. However, it can be hard
even for experienced pathologists because IM often occurs in mixed type or in hybrid type. Thus,
I believe that the determination of IM subtype should be backed up by ancillary studies such as
special stainings or other established IHC markers.
4. Hybrid-type IM is likely to express both CD10 and Das-1, which could confound the results.
I’m wondering whether there were no samples containing hybrid type IM.
5. In the lower panel of fig. 2B, authors claim that it is incomplete type IM, however, it seems
to be mixed type IM and Das-1 positive area is mostly complete IM, whereas incomplete type
IM portion is negative for Das-1.
assistant