Reviewer's report
Title:Role of myosin light chain kinase in intestinal epithelial barrier defects in a
rat model of bowel obstruction
Version: 1Date:24 November 2009
Reviewer: Stephan Samel
Reviewer's report:
Major Compulsory Revisions:
- Methods: (1) the authors ought to provide more details on experimental animals
(age), experimental procedures (e.g. central venous fluid substitution) and their
performance under surgery (monitoring of arterial and venous blood pressure,
mesenteric blood flow, oxygen saturation etc.). (2) Bowel obstruction is
associated with ischemia of the bowel and increased bacterial translocation (BT).
the experimental procedure of single bowel ligation may be insufficient to cause
critical bowel ischemia. histological findings close to the ligature may be
traumatic in origin. the authors ought to discuss their model in comparision to
other experimental settings(3) bacterial colonisation of the bowel ought to be
described and compared in detail.
Minor Essential Revisions
- the manuscript is to long and ought to be shorted by 25%.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:Role of myosin light chain kinase in intestinal epithelial barrier defects in a
rat model of bowel obstruction
Version: 1Date:24 November 2009
Reviewer: Stephan Samel
Reviewer's report:
Major Compulsory Revisions:
- Methods: (1) the authors ought to provide more details on experimental animals
(age), experimental procedures (e.g. central venous fluid substitution) and their
performance under surgery (monitoring of arterial and venous blood pressure,
mesenteric blood flow, oxygen saturation etc.). (2) Bowel obstruction is
associated with ischemia of the bowel and increased bacterial translocation (BT).
the experimental procedure of single bowel ligation may be insufficient to cause
critical bowel ischemia. histological findings close to the ligature may be
traumatic in origin. the authors ought to discuss their model in comparision to
other experimental settings(3) bacterial colonisation of the bowel ought to be
described and compared in detail.
Minor Essential Revisions
- the manuscript is to long and ought to be shorted by 25%.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:Role of myosin light chain kinase in intestinal epithelial barrier defects in a
rat model of bowel obstruction
Version: 1Date:24 November 2009
Reviewer: Stephan Samel
Reviewer's report:
Major Compulsory Revisions:
- Methods: (1) the authors ought to provide more details on experimental animals
(age), experimental procedures (e.g. central venous fluid substitution) and their
performance under surgery (monitoring of arterial and venous blood pressure,
mesenteric blood flow, oxygen saturation etc.). (2) Bowel obstruction is
associated with ischemia of the bowel and increased bacterial translocation (BT).
the experimental procedure of single bowel ligation may be insufficient to cause
critical bowel ischemia. histological findings close to the ligature may be
traumatic in origin. the authors ought to discuss their model in comparision to
other experimental settings(3) bacterial colonisation of the bowel ought to be
described and compared in detail.
Minor Essential Revisions
- the manuscript is to long and ought to be shorted by 25%.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests.
assistant