Title: Expression and function of myelin expression factor 2 in hepatocellular carcinoma
Version: 3 Date: 24 Aug 2022
Reviewer's report:
In this study, Zhang et al. aimed to find a biomarker for the diagnosis and prognosis of
HCC. They found that MYEF2 may be a potential target. Although finding biomarkers for the
diagnosis and prognosis of HCC is an urgent and crucial matter, and MYEF2 has indeed never
been reported in HCC, there are, however, several suggestions I’d like to give that may improve
the article.
1. Please provide a higher resolution of image 1C. The quality doesn’t meet the standard for
publication, in my opinion;
2. Is it necessary to present all OS, DSS, PFS, and RFS results in fig 1D-1G? I think it is a bit
redundant;
3. SK-Hep1 cells is endothelial origin, it is therefore not a typical HCC cell line. Please modify
the article accordingly or present results of a different cell line;
4. The details of the 142 pairs of tissue are unclear;
5. Please provide a higher resolution of fig 3B. I would also suggest the authors to provide one
consecutive image with both HCC tissue and adjacent tissue to increase the reliability of this
result;
6. In the methods, the authors wrote, “Cell migration time: SK-HEP-1 cells, 20 h; PLC/PRF/5
cells, 14 d. Cell invasion time: SK-HEP-1 cells, 24 h; PLC/PRF/5 cells, 15 d.”. Why does it
take two weeks for PLC/PRF/5 cells to migrate or invade? And id indeed this cell line takes
such a long time to migrate and invade, then how can it be distinguished from cell proliferation?
Besides, cell contours in fig 6D are unrecognizable.
Title: Expression and function of myelin expression factor 2 in hepatocellular carcinoma
Version: 3 Date: 24 Aug 2022
Reviewer's report:
In this study, Zhang et al. aimed to find a biomarker for the diagnosis and prognosis of
HCC. They found that MYEF2 may be a potential target. Although finding biomarkers for the
diagnosis and prognosis of HCC is an urgent and crucial matter, and MYEF2 has indeed never
been reported in HCC, there are, however, several suggestions I’d like to give that may improve
the article.
1. Please provide a higher resolution of image 1C. The quality doesn’t meet the standard for
publication, in my opinion;
2. Is it necessary to present all OS, DSS, PFS, and RFS results in fig 1D-1G? I think it is a bit
redundant;
3. SK-Hep1 cells is endothelial origin, it is therefore not a typical HCC cell line. Please modify
the article accordingly or present results of a different cell line;
4. The details of the 142 pairs of tissue are unclear;
5. Please provide a higher resolution of fig 3B. I would also suggest the authors to provide one
consecutive image with both HCC tissue and adjacent tissue to increase the reliability of this
result;
6. In the methods, the authors wrote, “Cell migration time: SK-HEP-1 cells, 20 h; PLC/PRF/5
cells, 14 d. Cell invasion time: SK-HEP-1 cells, 24 h; PLC/PRF/5 cells, 15 d.”. Why does it
take two weeks for PLC/PRF/5 cells to migrate or invade? And id indeed this cell line takes
such a long time to migrate and invade, then how can it be distinguished from cell proliferation?
Besides, cell contours in fig 6D are unrecognizable.
Please review this paper.
user
Title: Expression and function of myelin expression factor 2 in hepatocellular carcinoma
Version: 3 Date: 24 Aug 2022
Reviewer's report:
In this study, Zhang et al. aimed to find a biomarker for the diagnosis and prognosis of
HCC. They found that MYEF2 may be a potential target. Although finding biomarkers for the
diagnosis and prognosis of HCC is an urgent and crucial matter, and MYEF2 has indeed never
been reported in HCC, there are, however, several suggestions I’d like to give that may improve
the article.
1. Please provide a higher resolution of image 1C. The quality doesn’t meet the standard for
publication, in my opinion;
2. Is it necessary to present all OS, DSS, PFS, and RFS results in fig 1D-1G? I think it is a bit
redundant;
3. SK-Hep1 cells is endothelial origin, it is therefore not a typical HCC cell line. Please modify
the article accordingly or present results of a different cell line;
4. The details of the 142 pairs of tissue are unclear;
5. Please provide a higher resolution of fig 3B. I would also suggest the authors to provide one
consecutive image with both HCC tissue and adjacent tissue to increase the reliability of this
result;
6. In the methods, the authors wrote, “Cell migration time: SK-HEP-1 cells, 20 h; PLC/PRF/5
cells, 14 d. Cell invasion time: SK-HEP-1 cells, 24 h; PLC/PRF/5 cells, 15 d.”. Why does it
take two weeks for PLC/PRF/5 cells to migrate or invade? And id indeed this cell line takes
such a long time to migrate and invade, then how can it be distinguished from cell proliferation?
Besides, cell contours in fig 6D are unrecognizable.
assistant