Title: Biochemical and histopathological evidence for beneficial effects of empagliflozin
pretreatment on acetic acid-induced colitis in rats
Version: 2 Date: 27 Jan 2023
Reviewer's report:
"PEER REVIEWER ASSESSMENTS:
OBJECTIVE - Full research articles: is there a clear objective that addresses a testable research
question(s) (brief or other article types: is there a clear objective)?
Yes - there is a clear objective
DESIGN - Is the current approach (including controls and analysis protocols) appropriate for
the objective?
Yes - the approach is appropriate
EXECUTION - Are the experiments and analyses performed with technical rigor to allow
confidence in the results?
Yes - experiments and analyses were performed appropriately
STATISTICS - Is the use of statistics in the manuscript appropriate?
Yes - appropriate statistical analyses have been used in the study
INTERPRETATION - Is the current interpretation/discussion of the results reasonable and not
overstated?
Yes - the author's interpretation is reasonable
OVERALL MANUSCRIPT POTENTIAL - Is the current version of this work technically
sound? If not, can revisions be made to make the work technically sound?
Probably - with minor revisions
PEER REVIEWER COMMENTS:
GENERAL COMMENTS: The study was well conducted, although some improvements and
clarifications of information are required. The objectives of the research are clear, the study
design and execution are appropriate, and the interpretation of data is correct.
The manuscript reported the potential use of an anti-diabetic drug, empagliflozin, in the
treatment of ulcerative colitis (UC).
All the methods used are conventional and established methods.
This work is proposing empagliflozin as a new drug for the treatment of inflammatory bowel
disease (IBD).
Other researchers researching in this area (UC and IBD) may, among any other further studies,
compare and contrast the efficacy of existing treatments with empagliflozin to further ascertain
the potential of this drug in clinical practice.
REQUESTED REVISIONS:
There were numerous grammatical and typographical errors throughout the manuscript. The
authors should carefully proofread the manuscript before submission.
The Methodology section needs to include the strain, gender, and age of the animals.
The Discussion section can be improved - present in various paragraphs to improve the
readability of the manuscript. The authors may consider discussing the possible mechanism
underlying the beneficial effects of Empa on UC, and how does Empa, which act on a receptor
in the kidney, ameliorates oxidative stress in the colon.
It may be useful to include the health status of other organs following treatment with acetic acid
and Empa – would Empa also improve any pathological damages to these organs induced by
acetic acid?"
Title: Biochemical and histopathological evidence for beneficial effects of empagliflozin
pretreatment on acetic acid-induced colitis in rats
Version: 2 Date: 27 Jan 2023
Reviewer's report:
"PEER REVIEWER ASSESSMENTS:
OBJECTIVE - Full research articles: is there a clear objective that addresses a testable research
question(s) (brief or other article types: is there a clear objective)?
Yes - there is a clear objective
DESIGN - Is the current approach (including controls and analysis protocols) appropriate for
the objective?
Yes - the approach is appropriate
EXECUTION - Are the experiments and analyses performed with technical rigor to allow
confidence in the results?
Yes - experiments and analyses were performed appropriately
STATISTICS - Is the use of statistics in the manuscript appropriate?
Yes - appropriate statistical analyses have been used in the study
INTERPRETATION - Is the current interpretation/discussion of the results reasonable and not
overstated?
Yes - the author's interpretation is reasonable
OVERALL MANUSCRIPT POTENTIAL - Is the current version of this work technically
sound? If not, can revisions be made to make the work technically sound?
Probably - with minor revisions
PEER REVIEWER COMMENTS:
GENERAL COMMENTS: The study was well conducted, although some improvements and
clarifications of information are required. The objectives of the research are clear, the study
design and execution are appropriate, and the interpretation of data is correct.
The manuscript reported the potential use of an anti-diabetic drug, empagliflozin, in the
treatment of ulcerative colitis (UC).
All the methods used are conventional and established methods.
This work is proposing empagliflozin as a new drug for the treatment of inflammatory bowel
disease (IBD).
Other researchers researching in this area (UC and IBD) may, among any other further studies,
compare and contrast the efficacy of existing treatments with empagliflozin to further ascertain
the potential of this drug in clinical practice.
REQUESTED REVISIONS:
There were numerous grammatical and typographical errors throughout the manuscript. The
authors should carefully proofread the manuscript before submission.
The Methodology section needs to include the strain, gender, and age of the animals.
The Discussion section can be improved - present in various paragraphs to improve the
readability of the manuscript. The authors may consider discussing the possible mechanism
underlying the beneficial effects of Empa on UC, and how does Empa, which act on a receptor
in the kidney, ameliorates oxidative stress in the colon.
It may be useful to include the health status of other organs following treatment with acetic acid
and Empa – would Empa also improve any pathological damages to these organs induced by
acetic acid?"
Please review this paper.
user
Title: Biochemical and histopathological evidence for beneficial effects of empagliflozin
pretreatment on acetic acid-induced colitis in rats
Version: 2 Date: 27 Jan 2023
Reviewer's report:
"PEER REVIEWER ASSESSMENTS:
OBJECTIVE - Full research articles: is there a clear objective that addresses a testable research
question(s) (brief or other article types: is there a clear objective)?
Yes - there is a clear objective
DESIGN - Is the current approach (including controls and analysis protocols) appropriate for
the objective?
Yes - the approach is appropriate
EXECUTION - Are the experiments and analyses performed with technical rigor to allow
confidence in the results?
Yes - experiments and analyses were performed appropriately
STATISTICS - Is the use of statistics in the manuscript appropriate?
Yes - appropriate statistical analyses have been used in the study
INTERPRETATION - Is the current interpretation/discussion of the results reasonable and not
overstated?
Yes - the author's interpretation is reasonable
OVERALL MANUSCRIPT POTENTIAL - Is the current version of this work technically
sound? If not, can revisions be made to make the work technically sound?
Probably - with minor revisions
PEER REVIEWER COMMENTS:
GENERAL COMMENTS: The study was well conducted, although some improvements and
clarifications of information are required. The objectives of the research are clear, the study
design and execution are appropriate, and the interpretation of data is correct.
The manuscript reported the potential use of an anti-diabetic drug, empagliflozin, in the
treatment of ulcerative colitis (UC).
All the methods used are conventional and established methods.
This work is proposing empagliflozin as a new drug for the treatment of inflammatory bowel
disease (IBD).
Other researchers researching in this area (UC and IBD) may, among any other further studies,
compare and contrast the efficacy of existing treatments with empagliflozin to further ascertain
the potential of this drug in clinical practice.
REQUESTED REVISIONS:
There were numerous grammatical and typographical errors throughout the manuscript. The
authors should carefully proofread the manuscript before submission.
The Methodology section needs to include the strain, gender, and age of the animals.
The Discussion section can be improved - present in various paragraphs to improve the
readability of the manuscript. The authors may consider discussing the possible mechanism
underlying the beneficial effects of Empa on UC, and how does Empa, which act on a receptor
in the kidney, ameliorates oxidative stress in the colon.
It may be useful to include the health status of other organs following treatment with acetic acid
and Empa – would Empa also improve any pathological damages to these organs induced by
acetic acid?"
assistant