Reviewer's report
Title:Transglucosidase improves the gut microbiota profile of type 2 diabetes
mellitus patients
Version: 3Date:19 January 2013
Reviewer: Toshio Watanabe
Reviewer's report:
The authors examined effect of transglucosidase (TGD) on the gut microbiota in
patients with type 2 diabetes mellitus (T2DM) patients. They reported that
significant decreases in Clostridium cluster IVand Clostridium subcluster XIVa
components were observed in the T2DM patients compared with the healthy
individuals, whereas the Lactobacillales and Bifidobacterium populations
significantly increased in the T2DM patients They also demonstrated that after 12
month-treatment with TGD increased, the Bacteroides to Firmicutes ratio. There
are some new findings in this manuscript, but the data are insufficient to support
the author’s conclusions.
1. No data on body weight and blood glucose (or HbA1c) level after TGD
treatment were presented in the manuscript. These data were very important and
should be reported. Furthermore, the authors should examine the relation
between changes in body weight or blood glucose level and the gut microbiota.
2. Based on the data by cluster analyses, the authors described that TGD
modulated the gut microbiota profile in patients with T2DM. Were the changes in
the microbiota by TGD statistically significant? In other words, did the author
evaluate the differences in bacterial diversity between before and after TGD
treatment?
3. The authors should add the data of placebo group in Figure 3 which shows the
fecal bacteria structure before and after treatment.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I have no competing interests
Reviewer's report
Title:Transglucosidase improves the gut microbiota profile of type 2 diabetes
mellitus patients
Version: 3Date:19 January 2013
Reviewer: Toshio Watanabe
Reviewer's report:
The authors examined effect of transglucosidase (TGD) on the gut microbiota in
patients with type 2 diabetes mellitus (T2DM) patients. They reported that
significant decreases in Clostridium cluster IVand Clostridium subcluster XIVa
components were observed in the T2DM patients compared with the healthy
individuals, whereas the Lactobacillales and Bifidobacterium populations
significantly increased in the T2DM patients They also demonstrated that after 12
month-treatment with TGD increased, the Bacteroides to Firmicutes ratio. There
are some new findings in this manuscript, but the data are insufficient to support
the author’s conclusions.
1. No data on body weight and blood glucose (or HbA1c) level after TGD
treatment were presented in the manuscript. These data were very important and
should be reported. Furthermore, the authors should examine the relation
between changes in body weight or blood glucose level and the gut microbiota.
2. Based on the data by cluster analyses, the authors described that TGD
modulated the gut microbiota profile in patients with T2DM. Were the changes in
the microbiota by TGD statistically significant? In other words, did the author
evaluate the differences in bacterial diversity between before and after TGD
treatment?
3. The authors should add the data of placebo group in Figure 3 which shows the
fecal bacteria structure before and after treatment.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Transglucosidase improves the gut microbiota profile of type 2 diabetes
mellitus patients
Version: 3Date:19 January 2013
Reviewer: Toshio Watanabe
Reviewer's report:
The authors examined effect of transglucosidase (TGD) on the gut microbiota in
patients with type 2 diabetes mellitus (T2DM) patients. They reported that
significant decreases in Clostridium cluster IVand Clostridium subcluster XIVa
components were observed in the T2DM patients compared with the healthy
individuals, whereas the Lactobacillales and Bifidobacterium populations
significantly increased in the T2DM patients They also demonstrated that after 12
month-treatment with TGD increased, the Bacteroides to Firmicutes ratio. There
are some new findings in this manuscript, but the data are insufficient to support
the author’s conclusions.
1. No data on body weight and blood glucose (or HbA1c) level after TGD
treatment were presented in the manuscript. These data were very important and
should be reported. Furthermore, the authors should examine the relation
between changes in body weight or blood glucose level and the gut microbiota.
2. Based on the data by cluster analyses, the authors described that TGD
modulated the gut microbiota profile in patients with T2DM. Were the changes in
the microbiota by TGD statistically significant? In other words, did the author
evaluate the differences in bacterial diversity between before and after TGD
treatment?
3. The authors should add the data of placebo group in Figure 3 which shows the
fecal bacteria structure before and after treatment.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I have no competing interests
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