Reviewer ’s report  
 
Title: Clinical significance of UGT1A1 polymorphism and expression of ERCC1, BRCA1, 
TYMS, RRM1, TUBB3, STMN1 and TOP2A in gastric cancer  
 
Version: 0 Date: 23 Jun 2016  
 
Reviewer: Rong Jun Guo  
 
Reviewer's report:  
 
There are several essential concerning points:  
1) One of the authors' conclusions was "The genotype frequencies for the UGT1A1  
polymorphism at position -211 were associated with tubular adenocarcinoma".   There are 
concerning points regarding this conclusion:  the authors classified the cancers into high, 
moderate, low and  undifferentiated for their data analysis.  Tubular adenoc arcinoma is just a 
morphological feature of adenocarcinoma. It seems to me that the conclusion was not 
consistent with their analysis.  Moreover, the classification is very pathology oriented and 
needs well -trained histopathological pathologists involved.  I do not see any practicing 
pathologist involved.    
2) The manuscript was not well written, in terms of theory, rationale and hypothesis.  
3) The manuscript needs more editing in terms of English language.  
Are the methods appropriate and well described?  
If not, please spec ify what is required in your comments to the authors.  
 
Yes 
 
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
 
No 
 
Are the conclusions drawn adequately supported by the data shown?  
If not, please explain in your comments to the authors.  
 
No 
 
Are you able to assess any statistics in the manuscript or would you recommend an  
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects req uire further  
assessment in your comments to the editors.  
 
I recommend additional statistical review  
 
Quality of written English  
Please indicate the quality of language in the manuscript:  
 
Not suitable for publication unless extensively edited  
 
Declaration of competing interests  
Please complete a declaration of competing interests, considering the following questions:  
 
1. Have you in the past five years received reimbursements, fees, funding, or salary from an  
organisation that may in any way gain  or lose financially from the publication of this  
manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares in an organisation that may in any way gain or lose  
financially from the publication of this manuscript, either now or in the fut ure? 
 
3. Do you hold or are you currently applying for any patents relating to the content of the  
manuscript?  
 
4. Have you received reimbursements, fees, funding, or salary from an organization that 
holds  or has applied for patents relating to the content of  the manuscript?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have any non -financial competing interests in relation to this paper?  
 
If you can answer no to all of the above, write 'I declare that I have no competing interests' 
below.  If your reply is yes to any, please give details below.  
 
I declare that I have no competing interests.  
 
I agree to the open peer review policy of the journal. I understand that my name will be included  
on my report to the authors and, if the manuscript is acc epted for publication, my named report  
including any attachments I upload will be posted on the website along with the authors'  
responses. I agree for my report to be made available under an Open Access Creative Commons  
CC-BY license ( http://creativecommon s.org/licenses/by/4.0/ ). I understand that any comments  
which I do not wish to be included in my named report can be included as confidential comments  
to the editors, which will not be published.  
 
I agree to the open peer review policy of the journal  
Reviewer ’s report  
 
Title: Clinical significance of UGT1A1 polymorphism and expression of ERCC1, BRCA1, 
TYMS, RRM1, TUBB3, STMN1 and TOP2A in gastric cancer  
 
Version: 0 Date: 23 Jun 2016  
 
Reviewer: Rong Jun Guo  
 
Reviewer's report:  
 
There are several essential concerning points:  
1) One of the authors' conclusions was "The genotype frequencies for the UGT1A1  
polymorphism at position -211 were associated with tubular adenocarcinoma".   There are 
concerning points regarding this conclusion:  the authors classified the cancers into high, 
moderate, low and  undifferentiated for their data analysis.  Tubular adenoc arcinoma is just a 
morphological feature of adenocarcinoma. It seems to me that the conclusion was not 
consistent with their analysis.  Moreover, the classification is very pathology oriented and 
needs well -trained histopathological pathologists involved.  I do not see any practicing 
pathologist involved.    
2) The manuscript was not well written, in terms of theory, rationale and hypothesis.  
3) The manuscript needs more editing in terms of English language.  
Are the methods appropriate and well described?  
If not, please spec ify what is required in your comments to the authors.  
 
Yes 
 
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
 
No 
 
Are the conclusions drawn adequately supported by the data shown?  
If not, please explain in your comments to the authors.  
 
No 
 
Are you able to assess any statistics in the manuscript or would you recommend an  
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects req uire further  
assessment in your comments to the editors.  
 
I recommend additional statistical review  
 
Quality of written English  
Please indicate the quality of language in the manuscript:  
 
Not suitable for publication unless extensively edited  
 
Declaration of competing interests  
Please complete a declaration of competing interests, considering the following questions:  
 
1. Have you in the past five years received reimbursements, fees, funding, or salary from an  
organisation that may in any way gain  or lose financially from the publication of this  
manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares in an organisation that may in any way gain or lose  
financially from the publication of this manuscript, either now or in the fut ure? 
 
3. Do you hold or are you currently applying for any patents relating to the content of the  
manuscript?  
 
4. Have you received reimbursements, fees, funding, or salary from an organization that 
holds  or has applied for patents relating to the content of  the manuscript?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have any non -financial competing interests in relation to this paper?  
 
If you can answer no to all of the above, write 'I declare that I have no competing interests' 
below.  If your reply is yes to any, please give details below.  
 
I declare that I have no competing interests.  
 
I agree to the open peer review policy of the journal. I understand that my name will be included  
on my report to the authors and, if the manuscript is acc epted for publication, my named report  
including any attachments I upload will be posted on the website along with the authors'  
responses. I agree for my report to be made available under an Open Access Creative Commons  
CC-BY license ( http://creativecommon s.org/licenses/by/4.0/ ). I understand that any comments  
which I do not wish to be included in my named report can be included as confidential comments  
to the editors, which will not be published.  
 
I agree to the open peer review policy of the journal  
Please review this paper.
user
Reviewer ’s report  
 
Title: Clinical significance of UGT1A1 polymorphism and expression of ERCC1, BRCA1, 
TYMS, RRM1, TUBB3, STMN1 and TOP2A in gastric cancer  
 
Version: 0 Date: 23 Jun 2016  
 
Reviewer: Rong Jun Guo  
 
Reviewer's report:  
 
There are several essential concerning points:  
1) One of the authors' conclusions was "The genotype frequencies for the UGT1A1  
polymorphism at position -211 were associated with tubular adenocarcinoma".   There are 
concerning points regarding this conclusion:  the authors classified the cancers into high, 
moderate, low and  undifferentiated for their data analysis.  Tubular adenoc arcinoma is just a 
morphological feature of adenocarcinoma. It seems to me that the conclusion was not 
consistent with their analysis.  Moreover, the classification is very pathology oriented and 
needs well -trained histopathological pathologists involved.  I do not see any practicing 
pathologist involved.    
2) The manuscript was not well written, in terms of theory, rationale and hypothesis.  
3) The manuscript needs more editing in terms of English language.  
Are the methods appropriate and well described?  
If not, please spec ify what is required in your comments to the authors.  
 
Yes 
 
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
 
No 
 
Are the conclusions drawn adequately supported by the data shown?  
If not, please explain in your comments to the authors.  
 
No 
 
Are you able to assess any statistics in the manuscript or would you recommend an  
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects req uire further  
assessment in your comments to the editors.  
 
I recommend additional statistical review  
 
Quality of written English  
Please indicate the quality of language in the manuscript:  
 
Not suitable for publication unless extensively edited  
 
Declaration of competing interests  
Please complete a declaration of competing interests, considering the following questions:  
 
1. Have you in the past five years received reimbursements, fees, funding, or salary from an  
organisation that may in any way gain  or lose financially from the publication of this  
manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares in an organisation that may in any way gain or lose  
financially from the publication of this manuscript, either now or in the fut ure? 
 
3. Do you hold or are you currently applying for any patents relating to the content of the  
manuscript?  
 
4. Have you received reimbursements, fees, funding, or salary from an organization that 
holds  or has applied for patents relating to the content of  the manuscript?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have any non -financial competing interests in relation to this paper?  
 
If you can answer no to all of the above, write 'I declare that I have no competing interests' 
below.  If your reply is yes to any, please give details below.  
 
I declare that I have no competing interests.  
 
I agree to the open peer review policy of the journal. I understand that my name will be included  
on my report to the authors and, if the manuscript is acc epted for publication, my named report  
including any attachments I upload will be posted on the website along with the authors'  
responses. I agree for my report to be made available under an Open Access Creative Commons  
CC-BY license ( http://creativecommon s.org/licenses/by/4.0/ ). I understand that any comments  
which I do not wish to be included in my named report can be included as confidential comments  
to the editors, which will not be published.  
 
I agree to the open peer review policy of the journal  
assistant