Reviewer's report 
 Effects of a Cholesterol-Enriched Diet on Intestinal Smooth Muscle Contraction: Inhibition of Title: 
Muscarinic Receptors and their Disinhibition by the 5-HT4 Agonist - Tegaserod 
 3  12 January 2006 Version: Date: 
 David Schneider Reviewer: 
Reviewer's report: 
General 
All major compulsory and discretionary revisions have been satisfactorily addressed. There are a 
few Minor Essential Revisions still necessary before publication, but this reviewer finds the first 
revision acceptable for publication with minor edits. 
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Major Compulsory Revisions (that the author must respond to before a decision on publication can be reached) 
None. 
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Minor Essential Revisions (such as missing labels on figures, or the wrong use of a term, which the 
author can be trusted to correct) 
Regarding Minor Essential Revision 8: There remains a discrepancy in the number of animals 
reported to have been used. The authors have adequately explained why they have changed the 
total number of animals used from 96 to 64, and why 4 less animals were subsequently available for 
experimentation. The authors have changed the experimental design numbers per group from 19 to 
18. However, it seems that at least 72 animals must have been used given 4 experimental groups of 18. This must be clarified. 
Regarding Minor Essential Revision 23: The X-axis title in all figures should be “log[Carbachol](M)” 
and not “log[Carbachol].M” 
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Discretionary Revisions (which the author can choose to ignore) 
None. 
 Accept after minor essential revisions What next?: 
 An article whose findings are important to those with closely related research Level of interest: 
interests 
 Acceptable Quality of written English: 
 No Statistical review: 
Declaration of competing interests: 
'I declare that I have no competing interests. 
Reviewer's report 
 Effects of a Cholesterol-Enriched Diet on Intestinal Smooth Muscle Contraction: Inhibition of Title: 
Muscarinic Receptors and their Disinhibition by the 5-HT4 Agonist - Tegaserod 
 3  12 January 2006 Version: Date: 
 David Schneider Reviewer: 
Reviewer's report: 
General 
All major compulsory and discretionary revisions have been satisfactorily addressed. There are a 
few Minor Essential Revisions still necessary before publication, but this reviewer finds the first 
revision acceptable for publication with minor edits. 
------------------------------------------------------------------------------- 
Major Compulsory Revisions (that the author must respond to before a decision on publication can be reached) 
None. 
------------------------------------------------------------------------------- 
Minor Essential Revisions (such as missing labels on figures, or the wrong use of a term, which the 
author can be trusted to correct) 
Regarding Minor Essential Revision 8: There remains a discrepancy in the number of animals 
reported to have been used. The authors have adequately explained why they have changed the 
total number of animals used from 96 to 64, and why 4 less animals were subsequently available for 
experimentation. The authors have changed the experimental design numbers per group from 19 to 
18. However, it seems that at least 72 animals must have been used given 4 experimental groups of 18. This must be clarified. 
Regarding Minor Essential Revision 23: The X-axis title in all figures should be “log[Carbachol](M)” 
and not “log[Carbachol].M” 
------------------------------------------------------------------------------- 
Discretionary Revisions (which the author can choose to ignore) 
None. 
 Accept after minor essential revisions What next?: 
 An article whose findings are important to those with closely related research Level of interest: 
interests 
 Acceptable Quality of written English: 
 No Statistical review: 
Declaration of competing interests: 
'I declare that I have no competing interests. 
Please review this paper.
user
Reviewer's report 
 Effects of a Cholesterol-Enriched Diet on Intestinal Smooth Muscle Contraction: Inhibition of Title: 
Muscarinic Receptors and their Disinhibition by the 5-HT4 Agonist - Tegaserod 
 3  12 January 2006 Version: Date: 
 David Schneider Reviewer: 
Reviewer's report: 
General 
All major compulsory and discretionary revisions have been satisfactorily addressed. There are a 
few Minor Essential Revisions still necessary before publication, but this reviewer finds the first 
revision acceptable for publication with minor edits. 
------------------------------------------------------------------------------- 
Major Compulsory Revisions (that the author must respond to before a decision on publication can be reached) 
None. 
------------------------------------------------------------------------------- 
Minor Essential Revisions (such as missing labels on figures, or the wrong use of a term, which the 
author can be trusted to correct) 
Regarding Minor Essential Revision 8: There remains a discrepancy in the number of animals 
reported to have been used. The authors have adequately explained why they have changed the 
total number of animals used from 96 to 64, and why 4 less animals were subsequently available for 
experimentation. The authors have changed the experimental design numbers per group from 19 to 
18. However, it seems that at least 72 animals must have been used given 4 experimental groups of 18. This must be clarified. 
Regarding Minor Essential Revision 23: The X-axis title in all figures should be “log[Carbachol](M)” 
and not “log[Carbachol].M” 
------------------------------------------------------------------------------- 
Discretionary Revisions (which the author can choose to ignore) 
None. 
 Accept after minor essential revisions What next?: 
 An article whose findings are important to those with closely related research Level of interest: 
interests 
 Acceptable Quality of written English: 
 No Statistical review: 
Declaration of competing interests: 
'I declare that I have no competing interests. 
assistant