Title: Emodin Protects Against Severe Acute Pancreatitis-associated Acute Lung Injury by
Activating Nrf2/HO-1/GPX4 Signal and Inhibiting Ferroptosis in Vivo and in Vitro
Version: 2 Date: 06 Jan 2025
Reviewer:  Chuanhai Cao
Reviewer's report:
The manuscript submitted by Shen et al. to BMC Gastroenterology, titled “Emodin Protects
Against Severe Acute Pancreatitis-associated Acute Lung Injury by Activating Nrf2/HO-1/
GPX4 Signal and Inhibiting Ferroptosis in Vivo and in Vitro” presents findings on the use of
Emodin (EMO) as a preventive agent against SAP-ALI in both in vitro and in vivo models.
The study concludes that EMO effectively inhibits ferroptosis and modulates the Nrf2/HO-1/
GPX4 signaling pathway, thereby providing protection against SAP-ALI. This research is well-
structured, using both animal and cell models, with methods described that are clearly articulated
and easy to replicate. The results are presented comprehensively, accompanied by adequate
explanation and the conclusions are strictly adhered to the results. Overall, this is a very
nice research study and aligns well with the journal very well. I recommend accepting it for
publication, after making revision to the following points:
1. Change “This research was conducted to explore how EMO protects against lung injury in
SAP rats and alveolar epithelial cells, concentrating on the classical ferroptosis pathway” to
“This study was undertaken to investigate the protective effects of EMO against lung injury
in SAP rats and alveolar epithelial cells, with a particular focus on the classical ferroptosis
pathway”
2. Modify “In an in vivo study, forty male SD rats were split into five groups on average” to
“Forty SD rats were evenly split into five groups”
3. Revised the conclusions from” This study confirmed that EMO inhibited in vivo and in vitro
ferroptosis and modulated Nrf2/HO-1/GPX4 signaling to protect against SAP-ALI” to “Our
results demonstrated that EMO effectively inhibited ferroptosis both in vivo and in vitro, while
also modulating the Nrf2/HO-1/GPX4 signaling pathway to provide protection against SAP-
ALI”
4. Please ensure that all animal numbers are included in each figure legend, along with
indications of significance.
Title: Emodin Protects Against Severe Acute Pancreatitis-associated Acute Lung Injury by
Activating Nrf2/HO-1/GPX4 Signal and Inhibiting Ferroptosis in Vivo and in Vitro
Version: 2 Date: 06 Jan 2025
Reviewer:  Chuanhai Cao
Reviewer's report:
The manuscript submitted by Shen et al. to BMC Gastroenterology, titled “Emodin Protects
Against Severe Acute Pancreatitis-associated Acute Lung Injury by Activating Nrf2/HO-1/
GPX4 Signal and Inhibiting Ferroptosis in Vivo and in Vitro” presents findings on the use of
Emodin (EMO) as a preventive agent against SAP-ALI in both in vitro and in vivo models.
The study concludes that EMO effectively inhibits ferroptosis and modulates the Nrf2/HO-1/
GPX4 signaling pathway, thereby providing protection against SAP-ALI. This research is well-
structured, using both animal and cell models, with methods described that are clearly articulated
and easy to replicate. The results are presented comprehensively, accompanied by adequate
explanation and the conclusions are strictly adhered to the results. Overall, this is a very
nice research study and aligns well with the journal very well. I recommend accepting it for
publication, after making revision to the following points:
1. Change “This research was conducted to explore how EMO protects against lung injury in
SAP rats and alveolar epithelial cells, concentrating on the classical ferroptosis pathway” to
“This study was undertaken to investigate the protective effects of EMO against lung injury
in SAP rats and alveolar epithelial cells, with a particular focus on the classical ferroptosis
pathway”
2. Modify “In an in vivo study, forty male SD rats were split into five groups on average” to
“Forty SD rats were evenly split into five groups”
3. Revised the conclusions from” This study confirmed that EMO inhibited in vivo and in vitro
ferroptosis and modulated Nrf2/HO-1/GPX4 signaling to protect against SAP-ALI” to “Our
results demonstrated that EMO effectively inhibited ferroptosis both in vivo and in vitro, while
also modulating the Nrf2/HO-1/GPX4 signaling pathway to provide protection against SAP-
ALI”
4. Please ensure that all animal numbers are included in each figure legend, along with
indications of significance.
Please review this paper.
user
Title: Emodin Protects Against Severe Acute Pancreatitis-associated Acute Lung Injury by
Activating Nrf2/HO-1/GPX4 Signal and Inhibiting Ferroptosis in Vivo and in Vitro
Version: 2 Date: 06 Jan 2025
Reviewer:  Chuanhai Cao
Reviewer's report:
The manuscript submitted by Shen et al. to BMC Gastroenterology, titled “Emodin Protects
Against Severe Acute Pancreatitis-associated Acute Lung Injury by Activating Nrf2/HO-1/
GPX4 Signal and Inhibiting Ferroptosis in Vivo and in Vitro” presents findings on the use of
Emodin (EMO) as a preventive agent against SAP-ALI in both in vitro and in vivo models.
The study concludes that EMO effectively inhibits ferroptosis and modulates the Nrf2/HO-1/
GPX4 signaling pathway, thereby providing protection against SAP-ALI. This research is well-
structured, using both animal and cell models, with methods described that are clearly articulated
and easy to replicate. The results are presented comprehensively, accompanied by adequate
explanation and the conclusions are strictly adhered to the results. Overall, this is a very
nice research study and aligns well with the journal very well. I recommend accepting it for
publication, after making revision to the following points:
1. Change “This research was conducted to explore how EMO protects against lung injury in
SAP rats and alveolar epithelial cells, concentrating on the classical ferroptosis pathway” to
“This study was undertaken to investigate the protective effects of EMO against lung injury
in SAP rats and alveolar epithelial cells, with a particular focus on the classical ferroptosis
pathway”
2. Modify “In an in vivo study, forty male SD rats were split into five groups on average” to
“Forty SD rats were evenly split into five groups”
3. Revised the conclusions from” This study confirmed that EMO inhibited in vivo and in vitro
ferroptosis and modulated Nrf2/HO-1/GPX4 signaling to protect against SAP-ALI” to “Our
results demonstrated that EMO effectively inhibited ferroptosis both in vivo and in vitro, while
also modulating the Nrf2/HO-1/GPX4 signaling pathway to provide protection against SAP-
ALI”
4. Please ensure that all animal numbers are included in each figure legend, along with
indications of significance.
assistant