Reviewer's report
Title:Altered Expression of a Putative Progenitor Cell Marker DCAMKL1 in the
Gastric Mucosa in Regeneration, Metaplasia and Dysplasia
Version: 1Date:10 March 2010
Reviewer: Jian Lin Ren
Reviewer's report:
1. Authors well characterized the normal distribution of DCAMKL1-expressing
cells in gastric mucosa using electron microscopy; showed the distribution of
other proliferating, foveolar, mucous neck, parietal and chief cells using the
common specific molecular markers by immunohistochemical staining.
2. This study showed that expression patterns of DCAMKL1 both in normal and
different pathological states using immunohistochemical analysis and concluded
that these cell are belongs to a progenitor cell population. These results are
interesting and valuable information concerning the new molecular marker of
progenitor cells in gastrointestinal mucosa. However, there is one major issue
that should be addressed.
3. Authors followed up the distributive changes of DCAMKL1 and PCNA
expressing cells in the mucosa at different time points after ethonal injury in
Figure 3, but it would be essential to show the correlated H&E staining images
and quantitatively analyze the DCAMKL1 and PCNA expressing cell numbers at
5min, 1h, 6h, 24h and 96h after injury compared with the normal condition.
Quality of written English: Acceptable
Reviewer's report
Title:Altered Expression of a Putative Progenitor Cell Marker DCAMKL1 in the
Gastric Mucosa in Regeneration, Metaplasia and Dysplasia
Version: 1Date:10 March 2010
Reviewer: Jian Lin Ren
Reviewer's report:
1. Authors well characterized the normal distribution of DCAMKL1-expressing
cells in gastric mucosa using electron microscopy; showed the distribution of
other proliferating, foveolar, mucous neck, parietal and chief cells using the
common specific molecular markers by immunohistochemical staining.
2. This study showed that expression patterns of DCAMKL1 both in normal and
different pathological states using immunohistochemical analysis and concluded
that these cell are belongs to a progenitor cell population. These results are
interesting and valuable information concerning the new molecular marker of
progenitor cells in gastrointestinal mucosa. However, there is one major issue
that should be addressed.
3. Authors followed up the distributive changes of DCAMKL1 and PCNA
expressing cells in the mucosa at different time points after ethonal injury in
Figure 3, but it would be essential to show the correlated H&E staining images
and quantitatively analyze the DCAMKL1 and PCNA expressing cell numbers at
5min, 1h, 6h, 24h and 96h after injury compared with the normal condition.
Quality of written English: Acceptable
Please review this paper.
user
Reviewer's report
Title:Altered Expression of a Putative Progenitor Cell Marker DCAMKL1 in the
Gastric Mucosa in Regeneration, Metaplasia and Dysplasia
Version: 1Date:10 March 2010
Reviewer: Jian Lin Ren
Reviewer's report:
1. Authors well characterized the normal distribution of DCAMKL1-expressing
cells in gastric mucosa using electron microscopy; showed the distribution of
other proliferating, foveolar, mucous neck, parietal and chief cells using the
common specific molecular markers by immunohistochemical staining.
2. This study showed that expression patterns of DCAMKL1 both in normal and
different pathological states using immunohistochemical analysis and concluded
that these cell are belongs to a progenitor cell population. These results are
interesting and valuable information concerning the new molecular marker of
progenitor cells in gastrointestinal mucosa. However, there is one major issue
that should be addressed.
3. Authors followed up the distributive changes of DCAMKL1 and PCNA
expressing cells in the mucosa at different time points after ethonal injury in
Figure 3, but it would be essential to show the correlated H&E staining images
and quantitatively analyze the DCAMKL1 and PCNA expressing cell numbers at
5min, 1h, 6h, 24h and 96h after injury compared with the normal condition.
Quality of written English: Acceptable
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