Reviewer's report
Title:Longitudinal fluctuations in PD1 and PD-L1 expression in association with
changes in anti-viral immune response in chronic hepatitis B
Version: 5Date:31 July 2012
Reviewer: Christoph Neumann-Haefelin
Reviewer's report:
The authors have now provided a control staining without tetramer as well as
control tetramer stainings from HBV-negative controls. These data are shown in
the supplementary materials.
The validity of these controls is strongly reduced, since the gating procedure is
different from the gating procedure in Figure 1 - using the same gating cut-off for
the PE (tetramer) channel, none of the tests in Figure 1 would be positive.
In conclusion, the validity/ significance of the FACS data is limited. This needs to
be kept in mind when interpreting the data.
Level of interest: An article of limited interest
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Longitudinal fluctuations in PD1 and PD-L1 expression in association with
changes in anti-viral immune response in chronic hepatitis B
Version: 5Date:31 July 2012
Reviewer: Christoph Neumann-Haefelin
Reviewer's report:
The authors have now provided a control staining without tetramer as well as
control tetramer stainings from HBV-negative controls. These data are shown in
the supplementary materials.
The validity of these controls is strongly reduced, since the gating procedure is
different from the gating procedure in Figure 1 - using the same gating cut-off for
the PE (tetramer) channel, none of the tests in Figure 1 would be positive.
In conclusion, the validity/ significance of the FACS data is limited. This needs to
be kept in mind when interpreting the data.
Level of interest: An article of limited interest
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Longitudinal fluctuations in PD1 and PD-L1 expression in association with
changes in anti-viral immune response in chronic hepatitis B
Version: 5Date:31 July 2012
Reviewer: Christoph Neumann-Haefelin
Reviewer's report:
The authors have now provided a control staining without tetramer as well as
control tetramer stainings from HBV-negative controls. These data are shown in
the supplementary materials.
The validity of these controls is strongly reduced, since the gating procedure is
different from the gating procedure in Figure 1 - using the same gating cut-off for
the PE (tetramer) channel, none of the tests in Figure 1 would be positive.
In conclusion, the validity/ significance of the FACS data is limited. This needs to
be kept in mind when interpreting the data.
Level of interest: An article of limited interest
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
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