Reviewer's report
Title:1 MAC Isoflurane Preconditioning Induce Protective Effects of Heme
oxygenase-1 on Hepatic Ischemia Reperfusion in Rats
Version: 1Date:3 June 2010
Reviewer: Pronobesh Chattopadhyay
Reviewer's report:
Xin et al, shows novelty of Isoflurane Preconditioning anesthesia and Heme
oxygenase-1 relationship. Though prolong surgery Isoflurane is not
recommended because slow recovery rate. However the finding is novel and is
suitable for publication. I will be convinced and the paper may give good look if
the authors are followed this suggestion.
1. The authors should mention the HO-1 activity in ischemia followed reperfusion.
Introduction should be start with HO-1
2. Ischemia followed reperfusion should be noted uniformly IR or I/R through out
the paper.
3. The authors also suggested the IL-6 and IL-10 level along with neutrophil
accumulation.
4. Was any necrotic cells and any hyperplasia was observed? Should mentioned
in histopathology.
5. What was the viability of hepatocytes?
6. In research paper Phenobarbitone should not use in Ischemia group. May be
Phenobarbitone effect participate Isoflurane action
The paper has novelty with relation HO-1 expression and anesthesia. The paper
can be published with improving English and above said suggestion
Reviewer's report
Title:1 MAC Isoflurane Preconditioning Induce Protective Effects of Heme
oxygenase-1 on Hepatic Ischemia Reperfusion in Rats
Version: 1Date:3 June 2010
Reviewer: Pronobesh Chattopadhyay
Reviewer's report:
Xin et al, shows novelty of Isoflurane Preconditioning anesthesia and Heme
oxygenase-1 relationship. Though prolong surgery Isoflurane is not
recommended because slow recovery rate. However the finding is novel and is
suitable for publication. I will be convinced and the paper may give good look if
the authors are followed this suggestion.
1. The authors should mention the HO-1 activity in ischemia followed reperfusion.
Introduction should be start with HO-1
2. Ischemia followed reperfusion should be noted uniformly IR or I/R through out
the paper.
3. The authors also suggested the IL-6 and IL-10 level along with neutrophil
accumulation.
4. Was any necrotic cells and any hyperplasia was observed? Should mentioned
in histopathology.
5. What was the viability of hepatocytes?
6. In research paper Phenobarbitone should not use in Ischemia group. May be
Phenobarbitone effect participate Isoflurane action
The paper has novelty with relation HO-1 expression and anesthesia. The paper
can be published with improving English and above said suggestion
Please review this paper.
user
Reviewer's report
Title:1 MAC Isoflurane Preconditioning Induce Protective Effects of Heme
oxygenase-1 on Hepatic Ischemia Reperfusion in Rats
Version: 1Date:3 June 2010
Reviewer: Pronobesh Chattopadhyay
Reviewer's report:
Xin et al, shows novelty of Isoflurane Preconditioning anesthesia and Heme
oxygenase-1 relationship. Though prolong surgery Isoflurane is not
recommended because slow recovery rate. However the finding is novel and is
suitable for publication. I will be convinced and the paper may give good look if
the authors are followed this suggestion.
1. The authors should mention the HO-1 activity in ischemia followed reperfusion.
Introduction should be start with HO-1
2. Ischemia followed reperfusion should be noted uniformly IR or I/R through out
the paper.
3. The authors also suggested the IL-6 and IL-10 level along with neutrophil
accumulation.
4. Was any necrotic cells and any hyperplasia was observed? Should mentioned
in histopathology.
5. What was the viability of hepatocytes?
6. In research paper Phenobarbitone should not use in Ischemia group. May be
Phenobarbitone effect participate Isoflurane action
The paper has novelty with relation HO-1 expression and anesthesia. The paper
can be published with improving English and above said suggestion
assistant