Title: Optimization of the Cholesterol Gallstone Model in C57BL/6 Mice and Evaluation of
Lactobacillus Intervention Effects
Version: 2 Date: 13 Jan 2025
Reviewer's report:
In the study, authors optimized the cholesterol gallstone model of C57BL/6 mice and evaluated
the effect of probiotic intervention on the formation of cholestol stones, focusing on determining
the optimal feeding time for gallstone induction under a high-fat diet, as well as the optimal
timing and efficacy of probiotic intervention. Through a phased experimental design, this
study showed that the probiotic intervention significantly reduced the incidence and severity of
gallstones and improved liver pathology. The results suggest that the beneficial bacteria may
play a protective role by regulating bile acid and cholesterol metabolism, which has important
scientific innovation and clinical relevance.
The effect of intestinal probiotics on the formation of gallstones is the focus of current research,
but the literature on gallstone models is still limited. By inducing gallstone formation with a
high-fat diet, combined with probiotic interventions at different time periods (2 and 4 weeks of
preconditioning), this study provides data supporting time-dependent and dose-response effects
of probiotics. The protective effect of probiotics was systematically validated by a variety of
parameters, including body weight, liver pathology, and gallstone score. This study provides an
important basis for optimizing gallstone models and probiotic intervention strategies, and has
certain clinical translational potential.
Although studies have mentioned the regulatory role of probiotics on bile acid and cholesterol
metabolism, specific molecular pathways, such as key metabolic pathways and related
molecules, have not been mentioned. Despite the clinical relevance of probiotic preconditioning
and different doses of the study, the findings are based only on mouse models.
This study has certain innovative and scientific value, reasonable experimental design and
reliable data, and provides valuable insights for optimizing the mouse model of cholsterol
stone and probiotic preventive intervention. Based on its scientific value and potential clinical
relevance, I recommend this study for publication.
Title: Optimization of the Cholesterol Gallstone Model in C57BL/6 Mice and Evaluation of
Lactobacillus Intervention Effects
Version: 2 Date: 13 Jan 2025
Reviewer's report:
In the study, authors optimized the cholesterol gallstone model of C57BL/6 mice and evaluated
the effect of probiotic intervention on the formation of cholestol stones, focusing on determining
the optimal feeding time for gallstone induction under a high-fat diet, as well as the optimal
timing and efficacy of probiotic intervention. Through a phased experimental design, this
study showed that the probiotic intervention significantly reduced the incidence and severity of
gallstones and improved liver pathology. The results suggest that the beneficial bacteria may
play a protective role by regulating bile acid and cholesterol metabolism, which has important
scientific innovation and clinical relevance.
The effect of intestinal probiotics on the formation of gallstones is the focus of current research,
but the literature on gallstone models is still limited. By inducing gallstone formation with a
high-fat diet, combined with probiotic interventions at different time periods (2 and 4 weeks of
preconditioning), this study provides data supporting time-dependent and dose-response effects
of probiotics. The protective effect of probiotics was systematically validated by a variety of
parameters, including body weight, liver pathology, and gallstone score. This study provides an
important basis for optimizing gallstone models and probiotic intervention strategies, and has
certain clinical translational potential.
Although studies have mentioned the regulatory role of probiotics on bile acid and cholesterol
metabolism, specific molecular pathways, such as key metabolic pathways and related
molecules, have not been mentioned. Despite the clinical relevance of probiotic preconditioning
and different doses of the study, the findings are based only on mouse models.
This study has certain innovative and scientific value, reasonable experimental design and
reliable data, and provides valuable insights for optimizing the mouse model of cholsterol
stone and probiotic preventive intervention. Based on its scientific value and potential clinical
relevance, I recommend this study for publication.
Please review this paper.
user
Title: Optimization of the Cholesterol Gallstone Model in C57BL/6 Mice and Evaluation of
Lactobacillus Intervention Effects
Version: 2 Date: 13 Jan 2025
Reviewer's report:
In the study, authors optimized the cholesterol gallstone model of C57BL/6 mice and evaluated
the effect of probiotic intervention on the formation of cholestol stones, focusing on determining
the optimal feeding time for gallstone induction under a high-fat diet, as well as the optimal
timing and efficacy of probiotic intervention. Through a phased experimental design, this
study showed that the probiotic intervention significantly reduced the incidence and severity of
gallstones and improved liver pathology. The results suggest that the beneficial bacteria may
play a protective role by regulating bile acid and cholesterol metabolism, which has important
scientific innovation and clinical relevance.
The effect of intestinal probiotics on the formation of gallstones is the focus of current research,
but the literature on gallstone models is still limited. By inducing gallstone formation with a
high-fat diet, combined with probiotic interventions at different time periods (2 and 4 weeks of
preconditioning), this study provides data supporting time-dependent and dose-response effects
of probiotics. The protective effect of probiotics was systematically validated by a variety of
parameters, including body weight, liver pathology, and gallstone score. This study provides an
important basis for optimizing gallstone models and probiotic intervention strategies, and has
certain clinical translational potential.
Although studies have mentioned the regulatory role of probiotics on bile acid and cholesterol
metabolism, specific molecular pathways, such as key metabolic pathways and related
molecules, have not been mentioned. Despite the clinical relevance of probiotic preconditioning
and different doses of the study, the findings are based only on mouse models.
This study has certain innovative and scientific value, reasonable experimental design and
reliable data, and provides valuable insights for optimizing the mouse model of cholsterol
stone and probiotic preventive intervention. Based on its scientific value and potential clinical
relevance, I recommend this study for publication.
assistant