Reviewer's report
 Melatonin reduces TNF-a induced expression of MAdCAM-1 via inhibition of NF-kB. Title:
Authors:
Makotoh Sasaki MD (msasak@lsuhsc.edu )
Paul Jordan MD (pjorda1@lsuhsc.edu )
Takashi Joh MD (jalexa@lsuhsc.edu )
Makotoh Itoh MD (jalexa@lsuhsc.edu )
Michael Jenkins (michael.jenkins@virgin.net )
Kevin Pavlick PhD (kpavli@lsuhsc.edu )
Alireza Minagar MD (aminag@lsuhsc.edu )
J Steven Alexander PhD (jalexa@lsuhsc.edu )
 8  18 Apr 2002 Version: Date:
 Wayne Patton Reviewer:
 A paper whose findings are important to those with closely related research interests Level of interest:
 Accept after discretionary revisions Advice on publication:
The latest version is much 
improved over the other two and is responsive to my original criticisms. The 
abstract in the current version is a bit raw, but with minor English editing 
should be fine. Specifically, line 3 should have a comma after injury models, 
line three should end with "has" or "may have" instead of "have", line 5 should 
begin with "levels" or "expression", line 6 needs "was" after "expression", line 
7 needs the "m" in "um" capitalized, and line 9 should end in "expression". On page 2, line 5, "to" is required between "appears" and "promote" 
Under reagents on page 2 (line19), the Laemmli sample buffer should be 
referenced. 
On page 3, line 6, the ECL reagent is referred to for a second time. The city and state is different from the first mention on page 2, which has the correct 
information. I recommend deleting "(Amersham, La Jolla, CA)" on page 3. 
Competing interests:
The reviewer has no financial competing interests with regard to the paper. From 1987-1989, the 
reviewer did serve in the same academic laboratory as the senior author of the paper. This laboratory investigated cytoskeletal regulation of endothelial cell inflammation.
Reviewer's report
 Melatonin reduces TNF-a induced expression of MAdCAM-1 via inhibition of NF-kB. Title:
Authors:
Makotoh Sasaki MD (msasak@lsuhsc.edu )
Paul Jordan MD (pjorda1@lsuhsc.edu )
Takashi Joh MD (jalexa@lsuhsc.edu )
Makotoh Itoh MD (jalexa@lsuhsc.edu )
Michael Jenkins (michael.jenkins@virgin.net )
Kevin Pavlick PhD (kpavli@lsuhsc.edu )
Alireza Minagar MD (aminag@lsuhsc.edu )
J Steven Alexander PhD (jalexa@lsuhsc.edu )
 8  18 Apr 2002 Version: Date:
 Wayne Patton Reviewer:
 A paper whose findings are important to those with closely related research interests Level of interest:
 Accept after discretionary revisions Advice on publication:
The latest version is much 
improved over the other two and is responsive to my original criticisms. The 
abstract in the current version is a bit raw, but with minor English editing 
should be fine. Specifically, line 3 should have a comma after injury models, 
line three should end with "has" or "may have" instead of "have", line 5 should 
begin with "levels" or "expression", line 6 needs "was" after "expression", line 
7 needs the "m" in "um" capitalized, and line 9 should end in "expression". On page 2, line 5, "to" is required between "appears" and "promote" 
Under reagents on page 2 (line19), the Laemmli sample buffer should be 
referenced. 
On page 3, line 6, the ECL reagent is referred to for a second time. The city and state is different from the first mention on page 2, which has the correct 
information. I recommend deleting "(Amersham, La Jolla, CA)" on page 3. 
Competing interests:
The reviewer has no financial competing interests with regard to the paper. From 1987-1989, the 
reviewer did serve in the same academic laboratory as the senior author of the paper. This laboratory investigated cytoskeletal regulation of endothelial cell inflammation.
Please review this paper.
user
Reviewer's report
 Melatonin reduces TNF-a induced expression of MAdCAM-1 via inhibition of NF-kB. Title:
Authors:
Makotoh Sasaki MD (msasak@lsuhsc.edu )
Paul Jordan MD (pjorda1@lsuhsc.edu )
Takashi Joh MD (jalexa@lsuhsc.edu )
Makotoh Itoh MD (jalexa@lsuhsc.edu )
Michael Jenkins (michael.jenkins@virgin.net )
Kevin Pavlick PhD (kpavli@lsuhsc.edu )
Alireza Minagar MD (aminag@lsuhsc.edu )
J Steven Alexander PhD (jalexa@lsuhsc.edu )
 8  18 Apr 2002 Version: Date:
 Wayne Patton Reviewer:
 A paper whose findings are important to those with closely related research interests Level of interest:
 Accept after discretionary revisions Advice on publication:
The latest version is much 
improved over the other two and is responsive to my original criticisms. The 
abstract in the current version is a bit raw, but with minor English editing 
should be fine. Specifically, line 3 should have a comma after injury models, 
line three should end with "has" or "may have" instead of "have", line 5 should 
begin with "levels" or "expression", line 6 needs "was" after "expression", line 
7 needs the "m" in "um" capitalized, and line 9 should end in "expression". On page 2, line 5, "to" is required between "appears" and "promote" 
Under reagents on page 2 (line19), the Laemmli sample buffer should be 
referenced. 
On page 3, line 6, the ECL reagent is referred to for a second time. The city and state is different from the first mention on page 2, which has the correct 
information. I recommend deleting "(Amersham, La Jolla, CA)" on page 3. 
Competing interests:
The reviewer has no financial competing interests with regard to the paper. From 1987-1989, the 
reviewer did serve in the same academic laboratory as the senior author of the paper. This laboratory investigated cytoskeletal regulation of endothelial cell inflammation.
assistant