Title: CD45+ erythroid progenitor cells as potential biomarkers for disease progression in
hepatitis B virus-related acute-on-chronic liver failure
Version: 0 Date: 04 Apr 2025
Reviewer's report:
This authors aimed to evaluate the impact of CD45 + erythroid progenitor cells (EPCs) on
disease progression of hepatitis B virus-related  HBV-ACLF. The subject is interested and
the findings may be significant for prediction of the prognosis of HBV-ACLF.  However, the
followings need to be considered:
1.  The abnormal coagulation function, such as markable  abnormality of prothrombin time (PT),
prothrombin time activity (PTA) and international normalized ratio (INR),  is the most common
manifestation and related with the progress of ACLF.   In this study, according to figure 2,  the
CD45+ EPCs were not correlated with the above markers, how to explate the results?
2. The conclusion should focus  the discussion on the mechanism and  important value  of the
findings, rather than literature review. Please enrich the content of the discussion.
3. In figure 2 and figure 3, there are multiple graphs showed no significant between the CD45+
EPCs with the blood markers or complications and outcome, I wonder whether the figures are
necessary to be displayed.
Title: CD45+ erythroid progenitor cells as potential biomarkers for disease progression in
hepatitis B virus-related acute-on-chronic liver failure
Version: 0 Date: 04 Apr 2025
Reviewer's report:
This authors aimed to evaluate the impact of CD45 + erythroid progenitor cells (EPCs) on
disease progression of hepatitis B virus-related  HBV-ACLF. The subject is interested and
the findings may be significant for prediction of the prognosis of HBV-ACLF.  However, the
followings need to be considered:
1.  The abnormal coagulation function, such as markable  abnormality of prothrombin time (PT),
prothrombin time activity (PTA) and international normalized ratio (INR),  is the most common
manifestation and related with the progress of ACLF.   In this study, according to figure 2,  the
CD45+ EPCs were not correlated with the above markers, how to explate the results?
2. The conclusion should focus  the discussion on the mechanism and  important value  of the
findings, rather than literature review. Please enrich the content of the discussion.
3. In figure 2 and figure 3, there are multiple graphs showed no significant between the CD45+
EPCs with the blood markers or complications and outcome, I wonder whether the figures are
necessary to be displayed.
Please review this paper.
user
Title: CD45+ erythroid progenitor cells as potential biomarkers for disease progression in
hepatitis B virus-related acute-on-chronic liver failure
Version: 0 Date: 04 Apr 2025
Reviewer's report:
This authors aimed to evaluate the impact of CD45 + erythroid progenitor cells (EPCs) on
disease progression of hepatitis B virus-related  HBV-ACLF. The subject is interested and
the findings may be significant for prediction of the prognosis of HBV-ACLF.  However, the
followings need to be considered:
1.  The abnormal coagulation function, such as markable  abnormality of prothrombin time (PT),
prothrombin time activity (PTA) and international normalized ratio (INR),  is the most common
manifestation and related with the progress of ACLF.   In this study, according to figure 2,  the
CD45+ EPCs were not correlated with the above markers, how to explate the results?
2. The conclusion should focus  the discussion on the mechanism and  important value  of the
findings, rather than literature review. Please enrich the content of the discussion.
3. In figure 2 and figure 3, there are multiple graphs showed no significant between the CD45+
EPCs with the blood markers or complications and outcome, I wonder whether the figures are
necessary to be displayed.
assistant