Title: Association of alterations in transcriptomics and intestinal immune responses with
Bifidobacterium longum BAA2573 in improving dextran sulfate sodium- induced colitis
Version: 2 Date: 22 Apr 2025
Reviewer's report:
The authors demonstrated that pretreatment of B. longum BAA2573 demonstrated a promising
anti-inflammatory effect in DSS-induced colitis.  The present study was well organized and well
investigated, and will give us a new information, especially in the field of probiotics. To improve
the quality of this paper, the author should revise it according to the following suggestions;
1) RNA-seq and metabolome results suggest that B. longum BAA2573 induces anti-
inflammatory macrophages. FACS analysis of intestinal mucosal immune cells should be
performed to evaluate changes in M1 and M2 macrophages and the effects of BAA2573.
2) It is unclear what metabolites of B. longum BAA2573 have the effect of inducing
M2 macrophages. Can candidate molecules such as short-chain fatty acids and tryptophan
metabolites be identified from metabolomic metabolic analysis?
Title: Association of alterations in transcriptomics and intestinal immune responses with
Bifidobacterium longum BAA2573 in improving dextran sulfate sodium- induced colitis
Version: 2 Date: 22 Apr 2025
Reviewer's report:
The authors demonstrated that pretreatment of B. longum BAA2573 demonstrated a promising
anti-inflammatory effect in DSS-induced colitis.  The present study was well organized and well
investigated, and will give us a new information, especially in the field of probiotics. To improve
the quality of this paper, the author should revise it according to the following suggestions;
1) RNA-seq and metabolome results suggest that B. longum BAA2573 induces anti-
inflammatory macrophages. FACS analysis of intestinal mucosal immune cells should be
performed to evaluate changes in M1 and M2 macrophages and the effects of BAA2573.
2) It is unclear what metabolites of B. longum BAA2573 have the effect of inducing
M2 macrophages. Can candidate molecules such as short-chain fatty acids and tryptophan
metabolites be identified from metabolomic metabolic analysis?
Please review this paper.
user
Title: Association of alterations in transcriptomics and intestinal immune responses with
Bifidobacterium longum BAA2573 in improving dextran sulfate sodium- induced colitis
Version: 2 Date: 22 Apr 2025
Reviewer's report:
The authors demonstrated that pretreatment of B. longum BAA2573 demonstrated a promising
anti-inflammatory effect in DSS-induced colitis.  The present study was well organized and well
investigated, and will give us a new information, especially in the field of probiotics. To improve
the quality of this paper, the author should revise it according to the following suggestions;
1) RNA-seq and metabolome results suggest that B. longum BAA2573 induces anti-
inflammatory macrophages. FACS analysis of intestinal mucosal immune cells should be
performed to evaluate changes in M1 and M2 macrophages and the effects of BAA2573.
2) It is unclear what metabolites of B. longum BAA2573 have the effect of inducing
M2 macrophages. Can candidate molecules such as short-chain fatty acids and tryptophan
metabolites be identified from metabolomic metabolic analysis?
assistant