Title: Combined application of chondroitinase ABC and photobiomodulation with low-intensity
laser on the anal sphincter repair in rabbit
Version: 1 Date: 14 Sep 2021
Reviewer:  Nivaldo Antonio Parizotto
Reviewer's report:
The paper "Combined application of chondroitinase ABC and low-level laser on the anal
sphincter repair in rabbit" contains innovative data for the area and was carried out in accordance
with the appropriate animal management standards. The methods and procedures used were well
planned and the manuscript correctly records all the steps of the study, allowing its reproduction.
However, it is suggested to use the most current nomenclature for the use of low-intensity laser
therapy, which is photobiomodulation with low-intensity laser. This terminology was introduced
in 2015 and should be used to replace the previous terms, surpassed by the broader and more
correct characteristics of the term.
The results demonstrate an effective recovery of the anal sphincter of rabbits by associating
Low-level laser and chondroitinase ABC (ChABC) using functional and morphological analyzes
using immunohistochemistry, with evidence that gene expression is modified and contributes to
this muscle recovery analyses and their function.
Title: Combined application of chondroitinase ABC and photobiomodulation with low-intensity
laser on the anal sphincter repair in rabbit
Version: 1 Date: 14 Sep 2021
Reviewer:  Nivaldo Antonio Parizotto
Reviewer's report:
The paper "Combined application of chondroitinase ABC and low-level laser on the anal
sphincter repair in rabbit" contains innovative data for the area and was carried out in accordance
with the appropriate animal management standards. The methods and procedures used were well
planned and the manuscript correctly records all the steps of the study, allowing its reproduction.
However, it is suggested to use the most current nomenclature for the use of low-intensity laser
therapy, which is photobiomodulation with low-intensity laser. This terminology was introduced
in 2015 and should be used to replace the previous terms, surpassed by the broader and more
correct characteristics of the term.
The results demonstrate an effective recovery of the anal sphincter of rabbits by associating
Low-level laser and chondroitinase ABC (ChABC) using functional and morphological analyzes
using immunohistochemistry, with evidence that gene expression is modified and contributes to
this muscle recovery analyses and their function.
Please review this paper.
user
Title: Combined application of chondroitinase ABC and photobiomodulation with low-intensity
laser on the anal sphincter repair in rabbit
Version: 1 Date: 14 Sep 2021
Reviewer:  Nivaldo Antonio Parizotto
Reviewer's report:
The paper "Combined application of chondroitinase ABC and low-level laser on the anal
sphincter repair in rabbit" contains innovative data for the area and was carried out in accordance
with the appropriate animal management standards. The methods and procedures used were well
planned and the manuscript correctly records all the steps of the study, allowing its reproduction.
However, it is suggested to use the most current nomenclature for the use of low-intensity laser
therapy, which is photobiomodulation with low-intensity laser. This terminology was introduced
in 2015 and should be used to replace the previous terms, surpassed by the broader and more
correct characteristics of the term.
The results demonstrate an effective recovery of the anal sphincter of rabbits by associating
Low-level laser and chondroitinase ABC (ChABC) using functional and morphological analyzes
using immunohistochemistry, with evidence that gene expression is modified and contributes to
this muscle recovery analyses and their function.
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