Title: Efficacy of extracorporeal immunoadsorption therapy for removal of HBsAg
Version: 1 Date: 19 Feb 2025
Reviewer:  Yoshihiko Yano
Reviewer's report:
The manuscript shows the efficacy and safety of immunoadsorption therapy. It is potentially
interesting, but some modifications and additions are needed.
Major revision:
1. In the study using in vivo rabbit, blood samples were collected before and after the treatment.
This part is clinically important, but in the after treatment, how long does that effect last in the
long term effect? If it is a transient effect and there is no long term drug effect, it would not
be realistic. At least for HBs antigen and HBVDNA, the test results should be repeated and
presented several days and even months after the treatment.
2. For each experiment, the purpose of the experiment should be clearly described. Each
paragraph in the method section should describe why the study is being conducted.
Minor revision:
1. Figure 7 shows the laboratory values before and after the treatment. What would be the
changes in bilirubin level, which is an indicator of hemolysis, and globulin?
2. Why did author use V79 cells? It should be explained why this cell was used and whether the
results can be generalized to experiments with this cell.
3. Author should also describe in more detail how you measured HBS antigen, HBVDNA, etc.
Title: Efficacy of extracorporeal immunoadsorption therapy for removal of HBsAg
Version: 1 Date: 19 Feb 2025
Reviewer:  Yoshihiko Yano
Reviewer's report:
The manuscript shows the efficacy and safety of immunoadsorption therapy. It is potentially
interesting, but some modifications and additions are needed.
Major revision:
1. In the study using in vivo rabbit, blood samples were collected before and after the treatment.
This part is clinically important, but in the after treatment, how long does that effect last in the
long term effect? If it is a transient effect and there is no long term drug effect, it would not
be realistic. At least for HBs antigen and HBVDNA, the test results should be repeated and
presented several days and even months after the treatment.
2. For each experiment, the purpose of the experiment should be clearly described. Each
paragraph in the method section should describe why the study is being conducted.
Minor revision:
1. Figure 7 shows the laboratory values before and after the treatment. What would be the
changes in bilirubin level, which is an indicator of hemolysis, and globulin?
2. Why did author use V79 cells? It should be explained why this cell was used and whether the
results can be generalized to experiments with this cell.
3. Author should also describe in more detail how you measured HBS antigen, HBVDNA, etc.
Please review this paper.
user
Title: Efficacy of extracorporeal immunoadsorption therapy for removal of HBsAg
Version: 1 Date: 19 Feb 2025
Reviewer:  Yoshihiko Yano
Reviewer's report:
The manuscript shows the efficacy and safety of immunoadsorption therapy. It is potentially
interesting, but some modifications and additions are needed.
Major revision:
1. In the study using in vivo rabbit, blood samples were collected before and after the treatment.
This part is clinically important, but in the after treatment, how long does that effect last in the
long term effect? If it is a transient effect and there is no long term drug effect, it would not
be realistic. At least for HBs antigen and HBVDNA, the test results should be repeated and
presented several days and even months after the treatment.
2. For each experiment, the purpose of the experiment should be clearly described. Each
paragraph in the method section should describe why the study is being conducted.
Minor revision:
1. Figure 7 shows the laboratory values before and after the treatment. What would be the
changes in bilirubin level, which is an indicator of hemolysis, and globulin?
2. Why did author use V79 cells? It should be explained why this cell was used and whether the
results can be generalized to experiments with this cell.
3. Author should also describe in more detail how you measured HBS antigen, HBVDNA, etc.
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