Title: Optical biopsy for esophageal squamous cell neoplasia by using endocytoscopy
Version: 5 Date: 03 May 2022
Reviewer:  Akira Yokoyama
Reviewer's report:
Major comment
This paper shows that Endocytoscopy can differentiate dysplasia from cancer with extremely
high accuracy. The classification is clear in typical cases, but there are always borderline cases.
As Reviewer 2 pointed out, EC2 and EC3 are similar in typical cases, and the EC classification
cannot be validated correctly unless it is performed by a large number of endoscopists at other
institutions. Although this is a prospective, prospective study, it was conducted at only two
institutions, and although the number of endoscopists was not stated, it was probably conducted
by a small number of endoscopists, which does not make sense for a multicenter prospective
study as it should. We consider this to be a fatal problem in drawing conclusions for this paper.
On the other hand, as the results analyzed in this study design, we do not see any particular
problem with the analysis method, etc.
Minor comment
Endoscopist information should be included in the METHODS of the paper
Title: Optical biopsy for esophageal squamous cell neoplasia by using endocytoscopy
Version: 5 Date: 03 May 2022
Reviewer:  Akira Yokoyama
Reviewer's report:
Major comment
This paper shows that Endocytoscopy can differentiate dysplasia from cancer with extremely
high accuracy. The classification is clear in typical cases, but there are always borderline cases.
As Reviewer 2 pointed out, EC2 and EC3 are similar in typical cases, and the EC classification
cannot be validated correctly unless it is performed by a large number of endoscopists at other
institutions. Although this is a prospective, prospective study, it was conducted at only two
institutions, and although the number of endoscopists was not stated, it was probably conducted
by a small number of endoscopists, which does not make sense for a multicenter prospective
study as it should. We consider this to be a fatal problem in drawing conclusions for this paper.
On the other hand, as the results analyzed in this study design, we do not see any particular
problem with the analysis method, etc.
Minor comment
Endoscopist information should be included in the METHODS of the paper
Please review this paper.
user
Title: Optical biopsy for esophageal squamous cell neoplasia by using endocytoscopy
Version: 5 Date: 03 May 2022
Reviewer:  Akira Yokoyama
Reviewer's report:
Major comment
This paper shows that Endocytoscopy can differentiate dysplasia from cancer with extremely
high accuracy. The classification is clear in typical cases, but there are always borderline cases.
As Reviewer 2 pointed out, EC2 and EC3 are similar in typical cases, and the EC classification
cannot be validated correctly unless it is performed by a large number of endoscopists at other
institutions. Although this is a prospective, prospective study, it was conducted at only two
institutions, and although the number of endoscopists was not stated, it was probably conducted
by a small number of endoscopists, which does not make sense for a multicenter prospective
study as it should. We consider this to be a fatal problem in drawing conclusions for this paper.
On the other hand, as the results analyzed in this study design, we do not see any particular
problem with the analysis method, etc.
Minor comment
Endoscopist information should be included in the METHODS of the paper
assistant