Title: USP9X regulates the proliferation, survival, migration and invasion of gastric cancer cells
by stabilizing MTH1
Version: 1 Date: 01 May 2024
Reviewer:  Haruhiko Sugimura
Reviewer's report:
1. This is a sound analysis of USP9X and MTH1 in gastric cancer cell lines.
2. In terms of clinical relevance, the in silica data here is not impressive correlation bet ween
two gene expression.
3. As the authors are aware, the roles of these genes are known in other malignancies, thus , I
am afraid this is a kind of salami studies (just change the organs). The authors should particular
importance of this finding in gastric cancer.
4. State the significance or possible biological mechanism of MTH1 in gastric  carcinogenesis.
MUT1 is, I believe, one of base excision repair genes responsible to repair ROS DNA damage
in addition to MutYH  or others. Did you check the mutation burden when you put into MTH1
into gastric cancer cell lines? USP9X Downregulation (siRNA or CRISPR method) brought
about the same effect. Furthermore, is it unique to gastric cancer cell lines? How about distantly
related cancer such as endometrial cell cancer and lung cancer?
Title: USP9X regulates the proliferation, survival, migration and invasion of gastric cancer cells
by stabilizing MTH1
Version: 1 Date: 01 May 2024
Reviewer:  Haruhiko Sugimura
Reviewer's report:
1. This is a sound analysis of USP9X and MTH1 in gastric cancer cell lines.
2. In terms of clinical relevance, the in silica data here is not impressive correlation bet ween
two gene expression.
3. As the authors are aware, the roles of these genes are known in other malignancies, thus , I
am afraid this is a kind of salami studies (just change the organs). The authors should particular
importance of this finding in gastric cancer.
4. State the significance or possible biological mechanism of MTH1 in gastric  carcinogenesis.
MUT1 is, I believe, one of base excision repair genes responsible to repair ROS DNA damage
in addition to MutYH  or others. Did you check the mutation burden when you put into MTH1
into gastric cancer cell lines? USP9X Downregulation (siRNA or CRISPR method) brought
about the same effect. Furthermore, is it unique to gastric cancer cell lines? How about distantly
related cancer such as endometrial cell cancer and lung cancer?
Please review this paper.
user
Title: USP9X regulates the proliferation, survival, migration and invasion of gastric cancer cells
by stabilizing MTH1
Version: 1 Date: 01 May 2024
Reviewer:  Haruhiko Sugimura
Reviewer's report:
1. This is a sound analysis of USP9X and MTH1 in gastric cancer cell lines.
2. In terms of clinical relevance, the in silica data here is not impressive correlation bet ween
two gene expression.
3. As the authors are aware, the roles of these genes are known in other malignancies, thus , I
am afraid this is a kind of salami studies (just change the organs). The authors should particular
importance of this finding in gastric cancer.
4. State the significance or possible biological mechanism of MTH1 in gastric  carcinogenesis.
MUT1 is, I believe, one of base excision repair genes responsible to repair ROS DNA damage
in addition to MutYH  or others. Did you check the mutation burden when you put into MTH1
into gastric cancer cell lines? USP9X Downregulation (siRNA or CRISPR method) brought
about the same effect. Furthermore, is it unique to gastric cancer cell lines? How about distantly
related cancer such as endometrial cell cancer and lung cancer?
assistant