Title: CDX2 and Reg IV expression and correlation in gastric cancer
Version: 5 Date: 10 Feb 2021
Reviewer's report:
I think the authors have addressed the comments well and am happy to recommend this
manuscript for publication pending a couple of very minor issues which would benefit the
manuscript.
It might help future readers if some of the explanation was integrated into the text. I suspect
future readers may have the same questions. This is specifically referring to comments: 1 and 4
(simply explaining that its relative to beta actin in the legend would be sufficient)
I still have concerns about the Western blots representing the CDX2 protein for Figs 4 b. The
full blots now added as a supplementary file do not give much additional information. It would
be more convincing for me if a positive control sample could have been run alongside (perhaps
a sample from blot 4a (AGS cells which clearly expresses this protein at high levels)). Do the
authors have such a blot?
Similarly the same concerns exist for some of the RegIV blots appearing in the main figs. In this
case however the supp figures are better and could be used to try and obtain better resolution
images for the main figs. Specifically, the image for RegIV staining in fig 4a seems much less
clear than the full blot from the same fig (Supp 2a) and the same for RegIV staining in 4b which
also is clearer in Supp Fig 2c).
Title: CDX2 and Reg IV expression and correlation in gastric cancer
Version: 5 Date: 10 Feb 2021
Reviewer's report:
I think the authors have addressed the comments well and am happy to recommend this
manuscript for publication pending a couple of very minor issues which would benefit the
manuscript.
It might help future readers if some of the explanation was integrated into the text. I suspect
future readers may have the same questions. This is specifically referring to comments: 1 and 4
(simply explaining that its relative to beta actin in the legend would be sufficient)
I still have concerns about the Western blots representing the CDX2 protein for Figs 4 b. The
full blots now added as a supplementary file do not give much additional information. It would
be more convincing for me if a positive control sample could have been run alongside (perhaps
a sample from blot 4a (AGS cells which clearly expresses this protein at high levels)). Do the
authors have such a blot?
Similarly the same concerns exist for some of the RegIV blots appearing in the main figs. In this
case however the supp figures are better and could be used to try and obtain better resolution
images for the main figs. Specifically, the image for RegIV staining in fig 4a seems much less
clear than the full blot from the same fig (Supp 2a) and the same for RegIV staining in 4b which
also is clearer in Supp Fig 2c).
Please review this paper.
user
Title: CDX2 and Reg IV expression and correlation in gastric cancer
Version: 5 Date: 10 Feb 2021
Reviewer's report:
I think the authors have addressed the comments well and am happy to recommend this
manuscript for publication pending a couple of very minor issues which would benefit the
manuscript.
It might help future readers if some of the explanation was integrated into the text. I suspect
future readers may have the same questions. This is specifically referring to comments: 1 and 4
(simply explaining that its relative to beta actin in the legend would be sufficient)
I still have concerns about the Western blots representing the CDX2 protein for Figs 4 b. The
full blots now added as a supplementary file do not give much additional information. It would
be more convincing for me if a positive control sample could have been run alongside (perhaps
a sample from blot 4a (AGS cells which clearly expresses this protein at high levels)). Do the
authors have such a blot?
Similarly the same concerns exist for some of the RegIV blots appearing in the main figs. In this
case however the supp figures are better and could be used to try and obtain better resolution
images for the main figs. Specifically, the image for RegIV staining in fig 4a seems much less
clear than the full blot from the same fig (Supp 2a) and the same for RegIV staining in 4b which
also is clearer in Supp Fig 2c).
assistant