Reviewer's report
Title:The protective effect of VSL#3 on intestinal permeability in a rat model of
alcoholic liver disease
Version: 1Date:3 August 2013
Reviewer: akif alt&#305;nbas
Reviewer's report:
Dear Editor,
Chang et al. declared that they aimed to investigate the pathogenetic role of gut
– liver axis after acute alcohol administration in the alcohol related liver injury,
and the protective role of a probiotic mixture (VSL#3) and glutamin (an essential
amino acid resulted in intestinal mucosal atrophy with lacking).
To reach their aim, they administered alcohol via nasogastric tube to six different
groups of rat. Control group did not administered alcohol. To investigate the
protective role of VSL#3 and glutamin, they also administered VSL#3 and
glutamin seperately in to two groups, and to define their sinerjistic affect, both in
one group. And also, heat-killed VSL#3 and plasebo administered in another two
seperately groups. The investigated agents were all administered 30 min before
alcohol administration.
Alcohol related high small intestinal distrubtion was shown via
electron-microscopically, and the reduced mRNA of tight junction proteins by
using PCR and western blot.
And then, TNFalpha, endotoxin, ALT and AST levels in serum were all found to
be the highest level in only alcohol and plasebo groups, whereas the lowest level
in VSL#3 and glutamin combined group. And also, the levels of TNFalpha,
endotoxin, ALT and AST levels in serum were lower in the VSL#3, and glutamin
and heat-killed VSL#3 treated groups than alcohol group.
Minor essential revision
Even though the protocol of alcohol administration preffered in this study usually
resulted in alcohol related liver injury, the invetigators did not check out any
spesific findings of liver injury. So, they can only claim that VSL# and glutamin
may be protective role of acute alcohol consumption related intestinal injury, not
of liver injury. And also, it can not be understood why the investigators also
studied the severity of injury in the same rats.
In that reason, the authors should remove the words of liver injury from the text.
Instead, they pointed out that both VSL# and glutamin have protective role of
acute alcohol consumption related intestinal injury.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:The protective effect of VSL#3 on intestinal permeability in a rat model of
alcoholic liver disease
Version: 1Date:3 August 2013
Reviewer: akif alt&#305;nbas
Reviewer's report:
Dear Editor,
Chang et al. declared that they aimed to investigate the pathogenetic role of gut
– liver axis after acute alcohol administration in the alcohol related liver injury,
and the protective role of a probiotic mixture (VSL#3) and glutamin (an essential
amino acid resulted in intestinal mucosal atrophy with lacking).
To reach their aim, they administered alcohol via nasogastric tube to six different
groups of rat. Control group did not administered alcohol. To investigate the
protective role of VSL#3 and glutamin, they also administered VSL#3 and
glutamin seperately in to two groups, and to define their sinerjistic affect, both in
one group. And also, heat-killed VSL#3 and plasebo administered in another two
seperately groups. The investigated agents were all administered 30 min before
alcohol administration.
Alcohol related high small intestinal distrubtion was shown via
electron-microscopically, and the reduced mRNA of tight junction proteins by
using PCR and western blot.
And then, TNFalpha, endotoxin, ALT and AST levels in serum were all found to
be the highest level in only alcohol and plasebo groups, whereas the lowest level
in VSL#3 and glutamin combined group. And also, the levels of TNFalpha,
endotoxin, ALT and AST levels in serum were lower in the VSL#3, and glutamin
and heat-killed VSL#3 treated groups than alcohol group.
Minor essential revision
Even though the protocol of alcohol administration preffered in this study usually
resulted in alcohol related liver injury, the invetigators did not check out any
spesific findings of liver injury. So, they can only claim that VSL# and glutamin
may be protective role of acute alcohol consumption related intestinal injury, not
of liver injury. And also, it can not be understood why the investigators also
studied the severity of injury in the same rats.
In that reason, the authors should remove the words of liver injury from the text.
Instead, they pointed out that both VSL# and glutamin have protective role of
acute alcohol consumption related intestinal injury.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:The protective effect of VSL#3 on intestinal permeability in a rat model of
alcoholic liver disease
Version: 1Date:3 August 2013
Reviewer: akif alt&#305;nbas
Reviewer's report:
Dear Editor,
Chang et al. declared that they aimed to investigate the pathogenetic role of gut
– liver axis after acute alcohol administration in the alcohol related liver injury,
and the protective role of a probiotic mixture (VSL#3) and glutamin (an essential
amino acid resulted in intestinal mucosal atrophy with lacking).
To reach their aim, they administered alcohol via nasogastric tube to six different
groups of rat. Control group did not administered alcohol. To investigate the
protective role of VSL#3 and glutamin, they also administered VSL#3 and
glutamin seperately in to two groups, and to define their sinerjistic affect, both in
one group. And also, heat-killed VSL#3 and plasebo administered in another two
seperately groups. The investigated agents were all administered 30 min before
alcohol administration.
Alcohol related high small intestinal distrubtion was shown via
electron-microscopically, and the reduced mRNA of tight junction proteins by
using PCR and western blot.
And then, TNFalpha, endotoxin, ALT and AST levels in serum were all found to
be the highest level in only alcohol and plasebo groups, whereas the lowest level
in VSL#3 and glutamin combined group. And also, the levels of TNFalpha,
endotoxin, ALT and AST levels in serum were lower in the VSL#3, and glutamin
and heat-killed VSL#3 treated groups than alcohol group.
Minor essential revision
Even though the protocol of alcohol administration preffered in this study usually
resulted in alcohol related liver injury, the invetigators did not check out any
spesific findings of liver injury. So, they can only claim that VSL# and glutamin
may be protective role of acute alcohol consumption related intestinal injury, not
of liver injury. And also, it can not be understood why the investigators also
studied the severity of injury in the same rats.
In that reason, the authors should remove the words of liver injury from the text.
Instead, they pointed out that both VSL# and glutamin have protective role of
acute alcohol consumption related intestinal injury.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
assistant