Title: Self-Assembled Nanoparticles of Costunolide and Glycyrrhizic Acid for Enhanced
Ulcerative Colitis Treatment
Version: 3 Date: 16 Jun 2024
Reviewer's report:
Self-Assembled Nanoparticles of Costunolide and Glycyrrhizic Acid for Enhanced Ulcerative
Colitis Treatment
Type of Paper: Research Article
Abstract:
The abstract is well written but please add full forms of abbreviation in the abstract.
Introduction:
1) The introduction is well written. Please mention the studies that have already reported the
self-assembly properties of COS and GA because along with the other perspectives the whole
concept lies behind the self-assembly properties of COS and GA and then their mutual capability
to serve as an anti-inflammatory agent.
2) In the introduction section (line#64) Studies have demonstrated that COS alleviates symptoms
of UC in mice. Please add Reference studies
Methodology:
1) The methodology section is fine but in 2.1 Preparation of COS-GA NPs please rewrite it is
not clear.
2) Rewrite method 2.3.
3) 2.6: DSS-induced UC mice model establishment
Please clearly mention that how many study group were used (name it)
How many mice used in each group?
How the therapeutic group received the dose (dispersion media)
For how many days
The author mentioned that UC via DSS was induced for day 1-7. However, therapy was started
from day 3-10?. I believe at first the induction of colitis must be confirmed and then the treatment
must be initiated to monitor the overall progress of how the treatment is working
Result and discussion:
1) Section 3: Results and Discussion
and then section 4: Discussion (Please correct the titles)
2) In section 3.1 Preparation and morphological characterization of COS-GA NPs there is a
difference between TEM and Zeta Results as in zeta the average diameter is mentioned to be
369nm. Plz add a discussion
3) Add new figure for 1E.
4) Explanation required for section F of Figure 1. Two different groups are being plotted in the
graph.
5) In section 3.3,  30 minutes to assess the storage stability of the particles is not enough time,
the minimum period ranges from one week up to 3 months. Please check the literature and
accordingly report the stability study.
6) 3.4; Change uc to UC
7) The discussion is very short. The author need to discuss each result and compare their results
with similar studies.
The overall study is designed and conducted well. However, in my opinion, after reading this
article, I am thinking that what could be the mechanism of self-assembled NPs to relieve colitis.
These NPs will degrade in the Colitis site? Since these NPs will pass the stomach, small intestine
to reach the colon, any pH-dependent stability or degradation could be an important study. Please
add an explanation in the discussion part
Title: Self-Assembled Nanoparticles of Costunolide and Glycyrrhizic Acid for Enhanced
Ulcerative Colitis Treatment
Version: 3 Date: 16 Jun 2024
Reviewer's report:
Self-Assembled Nanoparticles of Costunolide and Glycyrrhizic Acid for Enhanced Ulcerative
Colitis Treatment
Type of Paper: Research Article
Abstract:
The abstract is well written but please add full forms of abbreviation in the abstract.
Introduction:
1) The introduction is well written. Please mention the studies that have already reported the
self-assembly properties of COS and GA because along with the other perspectives the whole
concept lies behind the self-assembly properties of COS and GA and then their mutual capability
to serve as an anti-inflammatory agent.
2) In the introduction section (line#64) Studies have demonstrated that COS alleviates symptoms
of UC in mice. Please add Reference studies
Methodology:
1) The methodology section is fine but in 2.1 Preparation of COS-GA NPs please rewrite it is
not clear.
2) Rewrite method 2.3.
3) 2.6: DSS-induced UC mice model establishment
Please clearly mention that how many study group were used (name it)
How many mice used in each group?
How the therapeutic group received the dose (dispersion media)
For how many days
The author mentioned that UC via DSS was induced for day 1-7. However, therapy was started
from day 3-10?. I believe at first the induction of colitis must be confirmed and then the treatment
must be initiated to monitor the overall progress of how the treatment is working
Result and discussion:
1) Section 3: Results and Discussion
and then section 4: Discussion (Please correct the titles)
2) In section 3.1 Preparation and morphological characterization of COS-GA NPs there is a
difference between TEM and Zeta Results as in zeta the average diameter is mentioned to be
369nm. Plz add a discussion
3) Add new figure for 1E.
4) Explanation required for section F of Figure 1. Two different groups are being plotted in the
graph.
5) In section 3.3,  30 minutes to assess the storage stability of the particles is not enough time,
the minimum period ranges from one week up to 3 months. Please check the literature and
accordingly report the stability study.
6) 3.4; Change uc to UC
7) The discussion is very short. The author need to discuss each result and compare their results
with similar studies.
The overall study is designed and conducted well. However, in my opinion, after reading this
article, I am thinking that what could be the mechanism of self-assembled NPs to relieve colitis.
These NPs will degrade in the Colitis site? Since these NPs will pass the stomach, small intestine
to reach the colon, any pH-dependent stability or degradation could be an important study. Please
add an explanation in the discussion part
Please review this paper.
user
Title: Self-Assembled Nanoparticles of Costunolide and Glycyrrhizic Acid for Enhanced
Ulcerative Colitis Treatment
Version: 3 Date: 16 Jun 2024
Reviewer's report:
Self-Assembled Nanoparticles of Costunolide and Glycyrrhizic Acid for Enhanced Ulcerative
Colitis Treatment
Type of Paper: Research Article
Abstract:
The abstract is well written but please add full forms of abbreviation in the abstract.
Introduction:
1) The introduction is well written. Please mention the studies that have already reported the
self-assembly properties of COS and GA because along with the other perspectives the whole
concept lies behind the self-assembly properties of COS and GA and then their mutual capability
to serve as an anti-inflammatory agent.
2) In the introduction section (line#64) Studies have demonstrated that COS alleviates symptoms
of UC in mice. Please add Reference studies
Methodology:
1) The methodology section is fine but in 2.1 Preparation of COS-GA NPs please rewrite it is
not clear.
2) Rewrite method 2.3.
3) 2.6: DSS-induced UC mice model establishment
Please clearly mention that how many study group were used (name it)
How many mice used in each group?
How the therapeutic group received the dose (dispersion media)
For how many days
The author mentioned that UC via DSS was induced for day 1-7. However, therapy was started
from day 3-10?. I believe at first the induction of colitis must be confirmed and then the treatment
must be initiated to monitor the overall progress of how the treatment is working
Result and discussion:
1) Section 3: Results and Discussion
and then section 4: Discussion (Please correct the titles)
2) In section 3.1 Preparation and morphological characterization of COS-GA NPs there is a
difference between TEM and Zeta Results as in zeta the average diameter is mentioned to be
369nm. Plz add a discussion
3) Add new figure for 1E.
4) Explanation required for section F of Figure 1. Two different groups are being plotted in the
graph.
5) In section 3.3,  30 minutes to assess the storage stability of the particles is not enough time,
the minimum period ranges from one week up to 3 months. Please check the literature and
accordingly report the stability study.
6) 3.4; Change uc to UC
7) The discussion is very short. The author need to discuss each result and compare their results
with similar studies.
The overall study is designed and conducted well. However, in my opinion, after reading this
article, I am thinking that what could be the mechanism of self-assembled NPs to relieve colitis.
These NPs will degrade in the Colitis site? Since these NPs will pass the stomach, small intestine
to reach the colon, any pH-dependent stability or degradation could be an important study. Please
add an explanation in the discussion part
assistant