Title: TRIM69: A Marker of Metastasis and Potential Sensitizer to 5-Fluorouracil and PD-1
Blockers in Colon Adenocarcinoma
Version: 1 Date: 16 Jun 2023
Reviewer's report:
1. The work by He et al aims to demonstrate that high TRIM69 expression has the potential to
enhance tumor sensitivity to 5-fluorouracil and programmed cell death protein 1 (PD-1) blockers
in COAD, but the evidence is unfortunately insufficient. The overall research work is relatively
simple. The authors only analyzed the correlation between the IC50 value of 5-fluorouracil, IPSs
of PD-1 blockers and the expression of TRIM69. However, there is a lack of direct experimental
evidence and clinical data to support its conclusions, that TRIM69 expression can affect drug
resistance.
2. In the second result, the authors analyzed the relationship between TRIP69 expression and
immune cell infiltration. Why did not analyze the correlation between T cells and TRIM69
expression? T cells play a crucial role in immunity.
3. Figure 1I-J did not show an association between TRIM69 expression and overall survival
(OS) or disease-free survival (DFS). Does it suggest that TRIM69 is of little clinical value in
COAD patients?
4. In Figure 6B, the authors should show how many samples were used for immunohistochemical
staining, and whether the TRIM69 expression was related to the clinical characteristics of the
COAD patients.
Title: TRIM69: A Marker of Metastasis and Potential Sensitizer to 5-Fluorouracil and PD-1
Blockers in Colon Adenocarcinoma
Version: 1 Date: 16 Jun 2023
Reviewer's report:
1. The work by He et al aims to demonstrate that high TRIM69 expression has the potential to
enhance tumor sensitivity to 5-fluorouracil and programmed cell death protein 1 (PD-1) blockers
in COAD, but the evidence is unfortunately insufficient. The overall research work is relatively
simple. The authors only analyzed the correlation between the IC50 value of 5-fluorouracil, IPSs
of PD-1 blockers and the expression of TRIM69. However, there is a lack of direct experimental
evidence and clinical data to support its conclusions, that TRIM69 expression can affect drug
resistance.
2. In the second result, the authors analyzed the relationship between TRIP69 expression and
immune cell infiltration. Why did not analyze the correlation between T cells and TRIM69
expression? T cells play a crucial role in immunity.
3. Figure 1I-J did not show an association between TRIM69 expression and overall survival
(OS) or disease-free survival (DFS). Does it suggest that TRIM69 is of little clinical value in
COAD patients?
4. In Figure 6B, the authors should show how many samples were used for immunohistochemical
staining, and whether the TRIM69 expression was related to the clinical characteristics of the
COAD patients.
Please review this paper.
user
Title: TRIM69: A Marker of Metastasis and Potential Sensitizer to 5-Fluorouracil and PD-1
Blockers in Colon Adenocarcinoma
Version: 1 Date: 16 Jun 2023
Reviewer's report:
1. The work by He et al aims to demonstrate that high TRIM69 expression has the potential to
enhance tumor sensitivity to 5-fluorouracil and programmed cell death protein 1 (PD-1) blockers
in COAD, but the evidence is unfortunately insufficient. The overall research work is relatively
simple. The authors only analyzed the correlation between the IC50 value of 5-fluorouracil, IPSs
of PD-1 blockers and the expression of TRIM69. However, there is a lack of direct experimental
evidence and clinical data to support its conclusions, that TRIM69 expression can affect drug
resistance.
2. In the second result, the authors analyzed the relationship between TRIP69 expression and
immune cell infiltration. Why did not analyze the correlation between T cells and TRIM69
expression? T cells play a crucial role in immunity.
3. Figure 1I-J did not show an association between TRIM69 expression and overall survival
(OS) or disease-free survival (DFS). Does it suggest that TRIM69 is of little clinical value in
COAD patients?
4. In Figure 6B, the authors should show how many samples were used for immunohistochemical
staining, and whether the TRIM69 expression was related to the clinical characteristics of the
COAD patients.
assistant