Title: Effect of disease duration on fecal biomarkers in ulcerative colitis: A prospective cohort
study
Version: 0 Date: 04 Jul 2022
Reviewer:  Sakuma Takahashi
Reviewer's report:
It is my pleasure to review the manuscript entitled "Effect of disease duration on fecal biomarkers
in ulcerative colitis: A prospective cohort study". However, I have several concerns that need
to be addressed before considering publication.
1) The analysis of "Cohort 1" is quite complicated and difficult to understand. The authors write
that "In the analysis of the long-term disease-duration group, the group with a disease duration
of 16 years or more was omitted because of their small sample sizes. Similarly, in the analysis
of the short-term disease-duration group, the group with a disease duration of #2 years, which
had small sample sizes, were omitted. In addition, the data for groups with disease durations of
20 and 30 years or more were evaluated since the sample sizes of groups with disease durations
of 16 to 38 years or more were small." on line 180-186, page 11-12.
These sentences may give arbitrary Impression for readers.
2) The authors write that "To avoid the effects of colonoscopic preparation or examination
on biomarkers, fecal samples for FC and FIT were collected the day before or after the
colonoscopy." on line 138-139, page 9.  I wonder FC and FIT collected the day after
the colonoscopy are influenced  colonoscopic preparation or examination, especially biopsy
procedures.
3) The authors write that "We suspect that DNA damage via this mechanism also alters
the inflammatory cytokine profile of the intestinal tract and may affect FC, which reflects
neutrophils in the intestinal tract." on line 326-328, page 20. It may be appropriate to include
references support this hypothesis.
Title: Effect of disease duration on fecal biomarkers in ulcerative colitis: A prospective cohort
study
Version: 0 Date: 04 Jul 2022
Reviewer:  Sakuma Takahashi
Reviewer's report:
It is my pleasure to review the manuscript entitled "Effect of disease duration on fecal biomarkers
in ulcerative colitis: A prospective cohort study". However, I have several concerns that need
to be addressed before considering publication.
1) The analysis of "Cohort 1" is quite complicated and difficult to understand. The authors write
that "In the analysis of the long-term disease-duration group, the group with a disease duration
of 16 years or more was omitted because of their small sample sizes. Similarly, in the analysis
of the short-term disease-duration group, the group with a disease duration of #2 years, which
had small sample sizes, were omitted. In addition, the data for groups with disease durations of
20 and 30 years or more were evaluated since the sample sizes of groups with disease durations
of 16 to 38 years or more were small." on line 180-186, page 11-12.
These sentences may give arbitrary Impression for readers.
2) The authors write that "To avoid the effects of colonoscopic preparation or examination
on biomarkers, fecal samples for FC and FIT were collected the day before or after the
colonoscopy." on line 138-139, page 9.  I wonder FC and FIT collected the day after
the colonoscopy are influenced  colonoscopic preparation or examination, especially biopsy
procedures.
3) The authors write that "We suspect that DNA damage via this mechanism also alters
the inflammatory cytokine profile of the intestinal tract and may affect FC, which reflects
neutrophils in the intestinal tract." on line 326-328, page 20. It may be appropriate to include
references support this hypothesis.
Please review this paper.
user
Title: Effect of disease duration on fecal biomarkers in ulcerative colitis: A prospective cohort
study
Version: 0 Date: 04 Jul 2022
Reviewer:  Sakuma Takahashi
Reviewer's report:
It is my pleasure to review the manuscript entitled "Effect of disease duration on fecal biomarkers
in ulcerative colitis: A prospective cohort study". However, I have several concerns that need
to be addressed before considering publication.
1) The analysis of "Cohort 1" is quite complicated and difficult to understand. The authors write
that "In the analysis of the long-term disease-duration group, the group with a disease duration
of 16 years or more was omitted because of their small sample sizes. Similarly, in the analysis
of the short-term disease-duration group, the group with a disease duration of #2 years, which
had small sample sizes, were omitted. In addition, the data for groups with disease durations of
20 and 30 years or more were evaluated since the sample sizes of groups with disease durations
of 16 to 38 years or more were small." on line 180-186, page 11-12.
These sentences may give arbitrary Impression for readers.
2) The authors write that "To avoid the effects of colonoscopic preparation or examination
on biomarkers, fecal samples for FC and FIT were collected the day before or after the
colonoscopy." on line 138-139, page 9.  I wonder FC and FIT collected the day after
the colonoscopy are influenced  colonoscopic preparation or examination, especially biopsy
procedures.
3) The authors write that "We suspect that DNA damage via this mechanism also alters
the inflammatory cytokine profile of the intestinal tract and may affect FC, which reflects
neutrophils in the intestinal tract." on line 326-328, page 20. It may be appropriate to include
references support this hypothesis.
assistant