Title: Triclosan Exposure Induced Disturbance of Gut Microbiota and Exaggerated
Experimental Colitis in Mice
Version: 7 Date: 26 Sep 2022
Reviewer's report:
In this paper, Jing et al. proposed to determine the effects of triclosan (TCS), an antibiotic heavily
used in consumer products, on intestinal inflammation and ulcerative colitis model. The authors
have addressed all my concerns, which have made this manuscript significantly clearer and with
more realistic interpretation of results.
The only thing missing still regards the unit for the panel B in Figures 1, 4, and 7. Authors
replied my previous comment saying the proteins were detected by semi-quantitative western
blot without concentration unit, but they should indicate the normalization. Please include in
the Figure "relative concentration" or include “Protein/#-actin ratio” and include in the figure
legend they have been normalized to #-actin concentration (see examples PMIDs: 28864290,
33420088, 24837592) as this information is not mentioned elsewhere.
Title: Triclosan Exposure Induced Disturbance of Gut Microbiota and Exaggerated
Experimental Colitis in Mice
Version: 7 Date: 26 Sep 2022
Reviewer's report:
In this paper, Jing et al. proposed to determine the effects of triclosan (TCS), an antibiotic heavily
used in consumer products, on intestinal inflammation and ulcerative colitis model. The authors
have addressed all my concerns, which have made this manuscript significantly clearer and with
more realistic interpretation of results.
The only thing missing still regards the unit for the panel B in Figures 1, 4, and 7. Authors
replied my previous comment saying the proteins were detected by semi-quantitative western
blot without concentration unit, but they should indicate the normalization. Please include in
the Figure "relative concentration" or include “Protein/#-actin ratio” and include in the figure
legend they have been normalized to #-actin concentration (see examples PMIDs: 28864290,
33420088, 24837592) as this information is not mentioned elsewhere.
Please review this paper.
user
Title: Triclosan Exposure Induced Disturbance of Gut Microbiota and Exaggerated
Experimental Colitis in Mice
Version: 7 Date: 26 Sep 2022
Reviewer's report:
In this paper, Jing et al. proposed to determine the effects of triclosan (TCS), an antibiotic heavily
used in consumer products, on intestinal inflammation and ulcerative colitis model. The authors
have addressed all my concerns, which have made this manuscript significantly clearer and with
more realistic interpretation of results.
The only thing missing still regards the unit for the panel B in Figures 1, 4, and 7. Authors
replied my previous comment saying the proteins were detected by semi-quantitative western
blot without concentration unit, but they should indicate the normalization. Please include in
the Figure "relative concentration" or include “Protein/#-actin ratio” and include in the figure
legend they have been normalized to #-actin concentration (see examples PMIDs: 28864290,
33420088, 24837592) as this information is not mentioned elsewhere.
assistant