Title: The contribution of lysophosphatidic acid receptors in the response of human lower
esophageal sphincter under the electrical field stimulation
Version: 1 Date: 24 Jan 2023
Reviewer's report:
This investigation is based on the fact that in the past, lysophosphatidic acid receptor agonists
have been shown to cause contraction in clasp and sling fibers. The researchers’ previous studies
found that 10-5mol/L selective LPA1, LPA2 receptor agonists, and selective LPA3 receptor
agonists can induce the maximum contraction effect of clasp and sling fiber muscle strips. In
addition, they found that non-specific antagonists’ have been shown to block this effect in human
clasp and sling. (reference 21).
Therefore, it appears that they set out to determine which specific lysophosphatidic acid receptor
antagonists were involved in blocking the contractile and relaxation responses. Combined with
the above research results, the investigators compared the response of human LES induced
by EFS, before and after the application of 10-5mol / L selective LPA1 and LPA3 receptor
antagonists, to clarify the role of LPA1 and LPA3 receptors in EFS. They found that there was
no significant difference in contractile or relaxation response to these two antagonists. However,
the authors don’t comment on why they didn’t try a selective LPA2 receptor antagonist. At a
minimum, I would suggest that the authors explain why this wasn’t done. Is it because there is no
LPA2 antagonist available? However, if an LPA2 receptor antagonist is available, the authors
should run those experiments to determine the effect, especially since the nonspecific antagonist
blocks the effect. Alternatively, if there is no selective LPA2 antagonist they could explain that
blocking the LPA1 and LPA3 receptors with selective antagonists has no effect, yet using a
nonspecific antagonist blocks the response. Therefore, the LP3 receptors must be responsible
for blocking the response. The investigators should have the paper reviewed for grammar and
syntax.
Title: The contribution of lysophosphatidic acid receptors in the response of human lower
esophageal sphincter under the electrical field stimulation
Version: 1 Date: 24 Jan 2023
Reviewer's report:
This investigation is based on the fact that in the past, lysophosphatidic acid receptor agonists
have been shown to cause contraction in clasp and sling fibers. The researchers’ previous studies
found that 10-5mol/L selective LPA1, LPA2 receptor agonists, and selective LPA3 receptor
agonists can induce the maximum contraction effect of clasp and sling fiber muscle strips. In
addition, they found that non-specific antagonists’ have been shown to block this effect in human
clasp and sling. (reference 21).
Therefore, it appears that they set out to determine which specific lysophosphatidic acid receptor
antagonists were involved in blocking the contractile and relaxation responses. Combined with
the above research results, the investigators compared the response of human LES induced
by EFS, before and after the application of 10-5mol / L selective LPA1 and LPA3 receptor
antagonists, to clarify the role of LPA1 and LPA3 receptors in EFS. They found that there was
no significant difference in contractile or relaxation response to these two antagonists. However,
the authors don’t comment on why they didn’t try a selective LPA2 receptor antagonist. At a
minimum, I would suggest that the authors explain why this wasn’t done. Is it because there is no
LPA2 antagonist available? However, if an LPA2 receptor antagonist is available, the authors
should run those experiments to determine the effect, especially since the nonspecific antagonist
blocks the effect. Alternatively, if there is no selective LPA2 antagonist they could explain that
blocking the LPA1 and LPA3 receptors with selective antagonists has no effect, yet using a
nonspecific antagonist blocks the response. Therefore, the LP3 receptors must be responsible
for blocking the response. The investigators should have the paper reviewed for grammar and
syntax.
Please review this paper.
user
Title: The contribution of lysophosphatidic acid receptors in the response of human lower
esophageal sphincter under the electrical field stimulation
Version: 1 Date: 24 Jan 2023
Reviewer's report:
This investigation is based on the fact that in the past, lysophosphatidic acid receptor agonists
have been shown to cause contraction in clasp and sling fibers. The researchers’ previous studies
found that 10-5mol/L selective LPA1, LPA2 receptor agonists, and selective LPA3 receptor
agonists can induce the maximum contraction effect of clasp and sling fiber muscle strips. In
addition, they found that non-specific antagonists’ have been shown to block this effect in human
clasp and sling. (reference 21).
Therefore, it appears that they set out to determine which specific lysophosphatidic acid receptor
antagonists were involved in blocking the contractile and relaxation responses. Combined with
the above research results, the investigators compared the response of human LES induced
by EFS, before and after the application of 10-5mol / L selective LPA1 and LPA3 receptor
antagonists, to clarify the role of LPA1 and LPA3 receptors in EFS. They found that there was
no significant difference in contractile or relaxation response to these two antagonists. However,
the authors don’t comment on why they didn’t try a selective LPA2 receptor antagonist. At a
minimum, I would suggest that the authors explain why this wasn’t done. Is it because there is no
LPA2 antagonist available? However, if an LPA2 receptor antagonist is available, the authors
should run those experiments to determine the effect, especially since the nonspecific antagonist
blocks the effect. Alternatively, if there is no selective LPA2 antagonist they could explain that
blocking the LPA1 and LPA3 receptors with selective antagonists has no effect, yet using a
nonspecific antagonist blocks the response. Therefore, the LP3 receptors must be responsible
for blocking the response. The investigators should have the paper reviewed for grammar and
syntax.
assistant