Reviewer's report
Title:HMGB1 cytoplasmic translocation in patients with acute liver failure
Version: 1Date:30 December 2010
Reviewer: Kohji Okamoto
Reviewer's report:
This is an interesting paper which adds to the understanding of the link hepatic
HMGB1 release, cytoplasmic translocation and ALF.
However, it seems that there is several points contrariety in this report.
1. In HepG2 cell culture, LPS or TNF induced HMGB1 cytolasmic translocation
and release. There is a difference of cytokine release between LPS and TNF.
Why was there the difference of cytokine release between two groups?
2. Furthermore, authors should make the additional experiment which IL8 and/or
GRO can induce HMGB1 release in HepG2.
3. HMGB1 cytoplasmic translocation in hepatocytes of ALF patients caused by
hepatitis B. These cytoplasmic HMGB1 might be a state that HMGB1, released
from nuclear of necrotic hepatic cells, was combined with a receptor (ex. RAGE,
TLR etc) of hepatic cell
4. In animal models, authors showed high percentage of hepatocytes with
HMGB1 cytoplasmic translocation at 3h after injection of D-GalN and LPS. At this
point, the rate of hepatic apoptosis was still low. How much was the ratio of
necrotic hepatocytes? It is known that HMGB1 is released from necrotic cells not
but apoptotic cells. (Scaffidi P et al. Nature 418:191-195,2002).
5. The details of the experiment of HepG2 were not written down in method of
Abstract.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:HMGB1 cytoplasmic translocation in patients with acute liver failure
Version: 1Date:30 December 2010
Reviewer: Kohji Okamoto
Reviewer's report:
This is an interesting paper which adds to the understanding of the link hepatic
HMGB1 release, cytoplasmic translocation and ALF.
However, it seems that there is several points contrariety in this report.
1. In HepG2 cell culture, LPS or TNF induced HMGB1 cytolasmic translocation
and release. There is a difference of cytokine release between LPS and TNF.
Why was there the difference of cytokine release between two groups?
2. Furthermore, authors should make the additional experiment which IL8 and/or
GRO can induce HMGB1 release in HepG2.
3. HMGB1 cytoplasmic translocation in hepatocytes of ALF patients caused by
hepatitis B. These cytoplasmic HMGB1 might be a state that HMGB1, released
from nuclear of necrotic hepatic cells, was combined with a receptor (ex. RAGE,
TLR etc) of hepatic cell
4. In animal models, authors showed high percentage of hepatocytes with
HMGB1 cytoplasmic translocation at 3h after injection of D-GalN and LPS. At this
point, the rate of hepatic apoptosis was still low. How much was the ratio of
necrotic hepatocytes? It is known that HMGB1 is released from necrotic cells not
but apoptotic cells. (Scaffidi P et al. Nature 418:191-195,2002).
5. The details of the experiment of HepG2 were not written down in method of
Abstract.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:HMGB1 cytoplasmic translocation in patients with acute liver failure
Version: 1Date:30 December 2010
Reviewer: Kohji Okamoto
Reviewer's report:
This is an interesting paper which adds to the understanding of the link hepatic
HMGB1 release, cytoplasmic translocation and ALF.
However, it seems that there is several points contrariety in this report.
1. In HepG2 cell culture, LPS or TNF induced HMGB1 cytolasmic translocation
and release. There is a difference of cytokine release between LPS and TNF.
Why was there the difference of cytokine release between two groups?
2. Furthermore, authors should make the additional experiment which IL8 and/or
GRO can induce HMGB1 release in HepG2.
3. HMGB1 cytoplasmic translocation in hepatocytes of ALF patients caused by
hepatitis B. These cytoplasmic HMGB1 might be a state that HMGB1, released
from nuclear of necrotic hepatic cells, was combined with a receptor (ex. RAGE,
TLR etc) of hepatic cell
4. In animal models, authors showed high percentage of hepatocytes with
HMGB1 cytoplasmic translocation at 3h after injection of D-GalN and LPS. At this
point, the rate of hepatic apoptosis was still low. How much was the ratio of
necrotic hepatocytes? It is known that HMGB1 is released from necrotic cells not
but apoptotic cells. (Scaffidi P et al. Nature 418:191-195,2002).
5. The details of the experiment of HepG2 were not written down in method of
Abstract.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
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