Title: Corticosteroid suppresses urea-cycle-related gene expressions in ornithine
transcarbamylase deficiency
Version: 7 Date: 10 Mar 2022
Reviewer's report:
BMC Gastroenterology   Imoto K paper                  01222022
The author adequately responded to the questions raised by the reviewer in the first draft.
The reviewer thinks the authors are requested to answer one more question before this  paper
is published in BMC Gastroenterology.
The author states “ARG1 mRNA expression level in Otcspf-ash mice may have been decreased
as compensation for the decrease of ornithine level” (p16, lines 13-15).
OTCD patients generally need a higher ornithine concentration than healthy individuals to
maximize limited citrulline synthesis. For this reason, it seems to the reviewer that OTCD
individuals (both human and mouse) are expected to have high ARG1 activity to supply enough
ornithine.
The reviewer thinks that to thoroughly elucidate effect of corticosteroid hormone on ureagenesis,
some more extended studies employing metabolomic, proteomic and molecular genetic
techniques is necessary. In this regard, the present article does not contain the whole information
that is necessary to address the research question in detail. Nonetheless, the reviewer supports
that this report can be published because  the reviewer believes that this work is the first part
that is essential for further extended research.  The reviewer  expects to  read further studies.
Title: Corticosteroid suppresses urea-cycle-related gene expressions in ornithine
transcarbamylase deficiency
Version: 7 Date: 10 Mar 2022
Reviewer's report:
BMC Gastroenterology   Imoto K paper                  01222022
The author adequately responded to the questions raised by the reviewer in the first draft.
The reviewer thinks the authors are requested to answer one more question before this  paper
is published in BMC Gastroenterology.
The author states “ARG1 mRNA expression level in Otcspf-ash mice may have been decreased
as compensation for the decrease of ornithine level” (p16, lines 13-15).
OTCD patients generally need a higher ornithine concentration than healthy individuals to
maximize limited citrulline synthesis. For this reason, it seems to the reviewer that OTCD
individuals (both human and mouse) are expected to have high ARG1 activity to supply enough
ornithine.
The reviewer thinks that to thoroughly elucidate effect of corticosteroid hormone on ureagenesis,
some more extended studies employing metabolomic, proteomic and molecular genetic
techniques is necessary. In this regard, the present article does not contain the whole information
that is necessary to address the research question in detail. Nonetheless, the reviewer supports
that this report can be published because  the reviewer believes that this work is the first part
that is essential for further extended research.  The reviewer  expects to  read further studies.
Please review this paper.
user
Title: Corticosteroid suppresses urea-cycle-related gene expressions in ornithine
transcarbamylase deficiency
Version: 7 Date: 10 Mar 2022
Reviewer's report:
BMC Gastroenterology   Imoto K paper                  01222022
The author adequately responded to the questions raised by the reviewer in the first draft.
The reviewer thinks the authors are requested to answer one more question before this  paper
is published in BMC Gastroenterology.
The author states “ARG1 mRNA expression level in Otcspf-ash mice may have been decreased
as compensation for the decrease of ornithine level” (p16, lines 13-15).
OTCD patients generally need a higher ornithine concentration than healthy individuals to
maximize limited citrulline synthesis. For this reason, it seems to the reviewer that OTCD
individuals (both human and mouse) are expected to have high ARG1 activity to supply enough
ornithine.
The reviewer thinks that to thoroughly elucidate effect of corticosteroid hormone on ureagenesis,
some more extended studies employing metabolomic, proteomic and molecular genetic
techniques is necessary. In this regard, the present article does not contain the whole information
that is necessary to address the research question in detail. Nonetheless, the reviewer supports
that this report can be published because  the reviewer believes that this work is the first part
that is essential for further extended research.  The reviewer  expects to  read further studies.
assistant