Title: Semantic clustering analysis of E3-ubiquitin ligases in gastrointestinal tract defines genes
ontology clusters with tissue expression patterns
Version: 8 Date: 19 Mar 2022
Reviewer's report:
Veronika et al. demonstrated that the expression profiling of GIT-specific UB-ligases shows the
unique expression profiling by the semantic clustering protocol. Their work may help to develop
the functional classification of Ub-ligases. However, some issues should be addressed before
publication. Specific comments are described below.
1. Fig. 2 shows the GO analysis. Authors collected the whole intestinal tissues for analysis. The
intestinal tissue includes different cell-types, epithelial cells, immune cells, and muscular cells
etc. I am just wondering if these analyses nicely work with different cell-types.
2. Authors analyzed the stomach and small intestines as well as colon in DSS-colitic mice.
Evidences that DSS induced the tissue damage and regeneration in stomach and small intestines
will reinforce their findings.
Title: Semantic clustering analysis of E3-ubiquitin ligases in gastrointestinal tract defines genes
ontology clusters with tissue expression patterns
Version: 8 Date: 19 Mar 2022
Reviewer's report:
Veronika et al. demonstrated that the expression profiling of GIT-specific UB-ligases shows the
unique expression profiling by the semantic clustering protocol. Their work may help to develop
the functional classification of Ub-ligases. However, some issues should be addressed before
publication. Specific comments are described below.
1. Fig. 2 shows the GO analysis. Authors collected the whole intestinal tissues for analysis. The
intestinal tissue includes different cell-types, epithelial cells, immune cells, and muscular cells
etc. I am just wondering if these analyses nicely work with different cell-types.
2. Authors analyzed the stomach and small intestines as well as colon in DSS-colitic mice.
Evidences that DSS induced the tissue damage and regeneration in stomach and small intestines
will reinforce their findings.
Please review this paper.
user
Title: Semantic clustering analysis of E3-ubiquitin ligases in gastrointestinal tract defines genes
ontology clusters with tissue expression patterns
Version: 8 Date: 19 Mar 2022
Reviewer's report:
Veronika et al. demonstrated that the expression profiling of GIT-specific UB-ligases shows the
unique expression profiling by the semantic clustering protocol. Their work may help to develop
the functional classification of Ub-ligases. However, some issues should be addressed before
publication. Specific comments are described below.
1. Fig. 2 shows the GO analysis. Authors collected the whole intestinal tissues for analysis. The
intestinal tissue includes different cell-types, epithelial cells, immune cells, and muscular cells
etc. I am just wondering if these analyses nicely work with different cell-types.
2. Authors analyzed the stomach and small intestines as well as colon in DSS-colitic mice.
Evidences that DSS induced the tissue damage and regeneration in stomach and small intestines
will reinforce their findings.
assistant