Title: Epidermal Growth Factor Receptor (EGFR) and SMAD4 negatively correlated in the
Progression of Gallbladder Adenocarcinoma in Eastern Indian Patients
Version: 4 Date: 08 Aug 2024
Reviewer's report:
Comments to the authors:
In the manuscript titled “Epidermal Growth Factor Receptor (EGFR) and SMAD4 negatively
correlated in the Progression of Gallbladder Adenocarcinoma in Eastern Indian Patients,” the
authors investigated the mutational proportion of the KRAS gene. They also examined the
expression and amplification patterns of the ERBB2/Her2-neu gene, along with the expression
patterns of six mostly dysregulated genes, the expression status of TGF-beta, and the status of
bacterial infections such as S. Typhi and H. pylori. Additionally, the study analyzed two SNPs of
the ERBB2/Her2-neu and CCND1 genes in the context of the Indian population of West Bengal
in Eastern India.
This manuscript is well-written, with a thorough examination and analysis of the subject matter.
It provides important insights. However, the following concerns should be addressed:
• The aim and objective are not clear. A precise statement outlining the main goals of the research
is necessary to provide readers with a clear understanding of the study’s purpose.
• The sample selection criteria should be detailed in the methodology section.
• The basis for selecting these specific genes should be explained. The rationale behind the
selection of the specific genes studied needs to be clearly explained.
Title: Epidermal Growth Factor Receptor (EGFR) and SMAD4 negatively correlated in the
Progression of Gallbladder Adenocarcinoma in Eastern Indian Patients
Version: 4 Date: 08 Aug 2024
Reviewer's report:
Comments to the authors:
In the manuscript titled “Epidermal Growth Factor Receptor (EGFR) and SMAD4 negatively
correlated in the Progression of Gallbladder Adenocarcinoma in Eastern Indian Patients,” the
authors investigated the mutational proportion of the KRAS gene. They also examined the
expression and amplification patterns of the ERBB2/Her2-neu gene, along with the expression
patterns of six mostly dysregulated genes, the expression status of TGF-beta, and the status of
bacterial infections such as S. Typhi and H. pylori. Additionally, the study analyzed two SNPs of
the ERBB2/Her2-neu and CCND1 genes in the context of the Indian population of West Bengal
in Eastern India.
This manuscript is well-written, with a thorough examination and analysis of the subject matter.
It provides important insights. However, the following concerns should be addressed:
• The aim and objective are not clear. A precise statement outlining the main goals of the research
is necessary to provide readers with a clear understanding of the study’s purpose.
• The sample selection criteria should be detailed in the methodology section.
• The basis for selecting these specific genes should be explained. The rationale behind the
selection of the specific genes studied needs to be clearly explained.
Please review this paper.
user
Title: Epidermal Growth Factor Receptor (EGFR) and SMAD4 negatively correlated in the
Progression of Gallbladder Adenocarcinoma in Eastern Indian Patients
Version: 4 Date: 08 Aug 2024
Reviewer's report:
Comments to the authors:
In the manuscript titled “Epidermal Growth Factor Receptor (EGFR) and SMAD4 negatively
correlated in the Progression of Gallbladder Adenocarcinoma in Eastern Indian Patients,” the
authors investigated the mutational proportion of the KRAS gene. They also examined the
expression and amplification patterns of the ERBB2/Her2-neu gene, along with the expression
patterns of six mostly dysregulated genes, the expression status of TGF-beta, and the status of
bacterial infections such as S. Typhi and H. pylori. Additionally, the study analyzed two SNPs of
the ERBB2/Her2-neu and CCND1 genes in the context of the Indian population of West Bengal
in Eastern India.
This manuscript is well-written, with a thorough examination and analysis of the subject matter.
It provides important insights. However, the following concerns should be addressed:
• The aim and objective are not clear. A precise statement outlining the main goals of the research
is necessary to provide readers with a clear understanding of the study’s purpose.
• The sample selection criteria should be detailed in the methodology section.
• The basis for selecting these specific genes should be explained. The rationale behind the
selection of the specific genes studied needs to be clearly explained.
assistant