Reviewer's report
Title:The effect of enteral and parenteral feeding on secretion of orexigenic
peptides in infants
Version: 1Date:14 May 2009
Reviewer: Mylene Perreault
Reviewer's report:
The current manuscript by Tomasik et al. reports that the secretion of orexigenic
peptides is regulated by the source of milk fed to infants. Although the different
feeding paradigms tested in this study had no significant effects on glucose
levels, they observed significant changes in ghrelin and orexin-a circulating
levels.
1)The study is well designed and provides novel information on the secretion of
orexigenic peptides in response to breast feeding versus other enteral or
parenteral feeding paradigm. The authors reported that plasma ghrelin levels
were regulated by the different paradigms studied. It is well established that
ghrelin circulates in two different forms: acylated and unacylated (desacyl). The
acylated form (known as active ghrelin) is thought to be essential for binding to
the growth hormone secretagogue receptor (GHSR). The authors need to
measure the levels of acylated ghrelin in those samples. Measurements of
growth hormone levels, stimulated upon ghrelin binding to its receptor, would
also be essential to determine if the observed increase/decrease in total ghrelin
has any relevant physiological effects.
2)The authors show for the first time that another orexigenic peptide, orexin-a, is
differently regulated by feeding human milk versus infant formula. Were other
neuroendocrine peptides, including CCK and GLP-1, also regulated? Please
include the data in this manuscript or discuss.
3)What was the proportion of males and females for each group and were there
any differences in orexigenic peptide secretion between genders? Please include
the proportions in the methods and add the data in the results section.
4)The results presented in this form are difficult to understand. I would suggest to
combine the fasting and postprandial graphs (figure 1 and 2) so the effects of
fasting and feeding on glucose, ghrelin and orexin-a are easily appreciated. The
legend to figures should be modified to reflect this change but also need to be
more descriptive.
5)Several references to figures or tables are missing or erroneous in the result
section.
6) This sentence needs to be fixed in the discussion. "The plasma levels of OXA
one hour after the feeding were similar to these observed in the fasting state,
except the breast-fed children showing INCREASE in its concentration." If
"increase" is really intended, then the sentence needs to be reformulated
because I do not agree with this conclusion.
7)The quality of the written english is for the most part acceptable but needs to
be addressed, specially for the following:
-Background section: "The breast feeding of newborns and infants declines a risk
of overweight since the second year, improves lipid profile and lowers blood
pressure in the further life [25, 26, 27, 28]. Probably, way of feeding alters
gut-brain axis and generates mentioned above short and long time differences in
the development."
-Background section: "Presented in this paper determination of the fasting and
postprandial levels of ghrelin and OXA in plasma in nursed infants, infants fed
with milk formula, babies maintaining on a hypoallergic, highly hydrolyzed diet as
well as in children on the total parenteral nutrition contributes to that problem."
-Discussion section:"They also received with nutrients similar energy (108 ± 20
kcal/kg/24h)."
-Discussion section:"Likely, the level of the hydrolysis of the proteins as well as
carbohydrates and additive of ghrelin itself could play a certain role."
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:The effect of enteral and parenteral feeding on secretion of orexigenic
peptides in infants
Version: 1Date:14 May 2009
Reviewer: Mylene Perreault
Reviewer's report:
The current manuscript by Tomasik et al. reports that the secretion of orexigenic
peptides is regulated by the source of milk fed to infants. Although the different
feeding paradigms tested in this study had no significant effects on glucose
levels, they observed significant changes in ghrelin and orexin-a circulating
levels.
1)The study is well designed and provides novel information on the secretion of
orexigenic peptides in response to breast feeding versus other enteral or
parenteral feeding paradigm. The authors reported that plasma ghrelin levels
were regulated by the different paradigms studied. It is well established that
ghrelin circulates in two different forms: acylated and unacylated (desacyl). The
acylated form (known as active ghrelin) is thought to be essential for binding to
the growth hormone secretagogue receptor (GHSR). The authors need to
measure the levels of acylated ghrelin in those samples. Measurements of
growth hormone levels, stimulated upon ghrelin binding to its receptor, would
also be essential to determine if the observed increase/decrease in total ghrelin
has any relevant physiological effects.
2)The authors show for the first time that another orexigenic peptide, orexin-a, is
differently regulated by feeding human milk versus infant formula. Were other
neuroendocrine peptides, including CCK and GLP-1, also regulated? Please
include the data in this manuscript or discuss.
3)What was the proportion of males and females for each group and were there
any differences in orexigenic peptide secretion between genders? Please include
the proportions in the methods and add the data in the results section.
4)The results presented in this form are difficult to understand. I would suggest to
combine the fasting and postprandial graphs (figure 1 and 2) so the effects of
fasting and feeding on glucose, ghrelin and orexin-a are easily appreciated. The
legend to figures should be modified to reflect this change but also need to be
more descriptive.
5)Several references to figures or tables are missing or erroneous in the result
section.
6) This sentence needs to be fixed in the discussion. "The plasma levels of OXA
one hour after the feeding were similar to these observed in the fasting state,
except the breast-fed children showing INCREASE in its concentration." If
"increase" is really intended, then the sentence needs to be reformulated
because I do not agree with this conclusion.
7)The quality of the written english is for the most part acceptable but needs to
be addressed, specially for the following:
-Background section: "The breast feeding of newborns and infants declines a risk
of overweight since the second year, improves lipid profile and lowers blood
pressure in the further life [25, 26, 27, 28]. Probably, way of feeding alters
gut-brain axis and generates mentioned above short and long time differences in
the development."
-Background section: "Presented in this paper determination of the fasting and
postprandial levels of ghrelin and OXA in plasma in nursed infants, infants fed
with milk formula, babies maintaining on a hypoallergic, highly hydrolyzed diet as
well as in children on the total parenteral nutrition contributes to that problem."
-Discussion section:"They also received with nutrients similar energy (108 ± 20
kcal/kg/24h)."
-Discussion section:"Likely, the level of the hydrolysis of the proteins as well as
carbohydrates and additive of ghrelin itself could play a certain role."
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:The effect of enteral and parenteral feeding on secretion of orexigenic
peptides in infants
Version: 1Date:14 May 2009
Reviewer: Mylene Perreault
Reviewer's report:
The current manuscript by Tomasik et al. reports that the secretion of orexigenic
peptides is regulated by the source of milk fed to infants. Although the different
feeding paradigms tested in this study had no significant effects on glucose
levels, they observed significant changes in ghrelin and orexin-a circulating
levels.
1)The study is well designed and provides novel information on the secretion of
orexigenic peptides in response to breast feeding versus other enteral or
parenteral feeding paradigm. The authors reported that plasma ghrelin levels
were regulated by the different paradigms studied. It is well established that
ghrelin circulates in two different forms: acylated and unacylated (desacyl). The
acylated form (known as active ghrelin) is thought to be essential for binding to
the growth hormone secretagogue receptor (GHSR). The authors need to
measure the levels of acylated ghrelin in those samples. Measurements of
growth hormone levels, stimulated upon ghrelin binding to its receptor, would
also be essential to determine if the observed increase/decrease in total ghrelin
has any relevant physiological effects.
2)The authors show for the first time that another orexigenic peptide, orexin-a, is
differently regulated by feeding human milk versus infant formula. Were other
neuroendocrine peptides, including CCK and GLP-1, also regulated? Please
include the data in this manuscript or discuss.
3)What was the proportion of males and females for each group and were there
any differences in orexigenic peptide secretion between genders? Please include
the proportions in the methods and add the data in the results section.
4)The results presented in this form are difficult to understand. I would suggest to
combine the fasting and postprandial graphs (figure 1 and 2) so the effects of
fasting and feeding on glucose, ghrelin and orexin-a are easily appreciated. The
legend to figures should be modified to reflect this change but also need to be
more descriptive.
5)Several references to figures or tables are missing or erroneous in the result
section.
6) This sentence needs to be fixed in the discussion. "The plasma levels of OXA
one hour after the feeding were similar to these observed in the fasting state,
except the breast-fed children showing INCREASE in its concentration." If
"increase" is really intended, then the sentence needs to be reformulated
because I do not agree with this conclusion.
7)The quality of the written english is for the most part acceptable but needs to
be addressed, specially for the following:
-Background section: "The breast feeding of newborns and infants declines a risk
of overweight since the second year, improves lipid profile and lowers blood
pressure in the further life [25, 26, 27, 28]. Probably, way of feeding alters
gut-brain axis and generates mentioned above short and long time differences in
the development."
-Background section: "Presented in this paper determination of the fasting and
postprandial levels of ghrelin and OXA in plasma in nursed infants, infants fed
with milk formula, babies maintaining on a hypoallergic, highly hydrolyzed diet as
well as in children on the total parenteral nutrition contributes to that problem."
-Discussion section:"They also received with nutrients similar energy (108 ± 20
kcal/kg/24h)."
-Discussion section:"Likely, the level of the hydrolysis of the proteins as well as
carbohydrates and additive of ghrelin itself could play a certain role."
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
assistant