Title: Quantitative proteomic studies of the intestinal mucosa provide new insights into the
molecular mechanism of ulcerative colitis
Version: 0 Date: 21 Sep 2024
Reviewer's report:
This article presents a unique study that delves into the pathogenesis of ulcerative colitis (UC)
through the innovative quantitative proteome analysis of the intestinal mucosa.
The authors described collecting mucosa from non-inflamed and inflamed areas and using it for
proteome analysis, but was the presence of inflammation determined endoscopically? Was the
inflammation in the biopsied tissue assessed histologically? Please describe clearly.
It is important to note that drugs administered to patients can influence the expression of
molecules in the mucosa, including cytokines. Therefore, the authors should consider the drugs
used in the enrolled patient and interpret the results obtained. Please discuss in the Discussion.
The duration of the disease, not age, also causes differences in the molecules expressed in the
mucosa. The authors need to take this into account when interpreting the results.
Interestingly, the present study identified enhanced expression of TXNDC5 in the colonic
mucosa of UC. However, the mechanism by which TXNDC5 is upregulated in the mucosa of
UC has yet to be investigated in detail. A detailed study of this mechanism using suitable cell
lines is needed.
Title: Quantitative proteomic studies of the intestinal mucosa provide new insights into the
molecular mechanism of ulcerative colitis
Version: 0 Date: 21 Sep 2024
Reviewer's report:
This article presents a unique study that delves into the pathogenesis of ulcerative colitis (UC)
through the innovative quantitative proteome analysis of the intestinal mucosa.
The authors described collecting mucosa from non-inflamed and inflamed areas and using it for
proteome analysis, but was the presence of inflammation determined endoscopically? Was the
inflammation in the biopsied tissue assessed histologically? Please describe clearly.
It is important to note that drugs administered to patients can influence the expression of
molecules in the mucosa, including cytokines. Therefore, the authors should consider the drugs
used in the enrolled patient and interpret the results obtained. Please discuss in the Discussion.
The duration of the disease, not age, also causes differences in the molecules expressed in the
mucosa. The authors need to take this into account when interpreting the results.
Interestingly, the present study identified enhanced expression of TXNDC5 in the colonic
mucosa of UC. However, the mechanism by which TXNDC5 is upregulated in the mucosa of
UC has yet to be investigated in detail. A detailed study of this mechanism using suitable cell
lines is needed.
Please review this paper.
user
Title: Quantitative proteomic studies of the intestinal mucosa provide new insights into the
molecular mechanism of ulcerative colitis
Version: 0 Date: 21 Sep 2024
Reviewer's report:
This article presents a unique study that delves into the pathogenesis of ulcerative colitis (UC)
through the innovative quantitative proteome analysis of the intestinal mucosa.
The authors described collecting mucosa from non-inflamed and inflamed areas and using it for
proteome analysis, but was the presence of inflammation determined endoscopically? Was the
inflammation in the biopsied tissue assessed histologically? Please describe clearly.
It is important to note that drugs administered to patients can influence the expression of
molecules in the mucosa, including cytokines. Therefore, the authors should consider the drugs
used in the enrolled patient and interpret the results obtained. Please discuss in the Discussion.
The duration of the disease, not age, also causes differences in the molecules expressed in the
mucosa. The authors need to take this into account when interpreting the results.
Interestingly, the present study identified enhanced expression of TXNDC5 in the colonic
mucosa of UC. However, the mechanism by which TXNDC5 is upregulated in the mucosa of
UC has yet to be investigated in detail. A detailed study of this mechanism using suitable cell
lines is needed.
assistant