Reviewer's report
Title:The gene-reduction effect of chromosomal losses detected in gastric
cancers
Version: 1Date:19 August 2010
Reviewer: Jun Yu
Reviewer's report:
This manuscript reported the cell-adverse effect of gene reduction in relation to
gastric cancer differentiation types. They demonstrated that detection of loss of
heterozygosity using microsatellite analysis may have the potential as a method
of pretreatment genetic diagnosis marker. They concluded that the cell-adverse
effect of gene reduction is more tolerated in intestinal-type gastric cancers than in
diffuse-type cancers, and the loss of high-dose genes is associated with
hematogenous metastasis. The study is generally well designed. However, there
are several major comments should be addressed:
Major Compulsory Revisions
1) As shown in Table 3, in high-risk genotypes, age is positively correlated with
LOH level. When it comes to low-risk genotypes, age is negatively correlated
with LOH level. This phenomenon should be discussed accordingly?
2)The age and gender should be included in the models of multivariate analysis,
no matter they were significant or not.
Minor Revisions
1) Page8, Paragraph 1, Line 1: “One hundred forty-five” should be “One hundred
and forty-five”.
2) Page8, Paragraph 1, Line 4: “One hundred sixteen” should be “One hundred
and sixteen”.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Reviewer's report
Title:The gene-reduction effect of chromosomal losses detected in gastric
cancers
Version: 1Date:19 August 2010
Reviewer: Jun Yu
Reviewer's report:
This manuscript reported the cell-adverse effect of gene reduction in relation to
gastric cancer differentiation types. They demonstrated that detection of loss of
heterozygosity using microsatellite analysis may have the potential as a method
of pretreatment genetic diagnosis marker. They concluded that the cell-adverse
effect of gene reduction is more tolerated in intestinal-type gastric cancers than in
diffuse-type cancers, and the loss of high-dose genes is associated with
hematogenous metastasis. The study is generally well designed. However, there
are several major comments should be addressed:
Major Compulsory Revisions
1) As shown in Table 3, in high-risk genotypes, age is positively correlated with
LOH level. When it comes to low-risk genotypes, age is negatively correlated
with LOH level. This phenomenon should be discussed accordingly?
2)The age and gender should be included in the models of multivariate analysis,
no matter they were significant or not.
Minor Revisions
1) Page8, Paragraph 1, Line 1: “One hundred forty-five” should be “One hundred
and forty-five”.
2) Page8, Paragraph 1, Line 4: “One hundred sixteen” should be “One hundred
and sixteen”.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Please review this paper.
user
Reviewer's report
Title:The gene-reduction effect of chromosomal losses detected in gastric
cancers
Version: 1Date:19 August 2010
Reviewer: Jun Yu
Reviewer's report:
This manuscript reported the cell-adverse effect of gene reduction in relation to
gastric cancer differentiation types. They demonstrated that detection of loss of
heterozygosity using microsatellite analysis may have the potential as a method
of pretreatment genetic diagnosis marker. They concluded that the cell-adverse
effect of gene reduction is more tolerated in intestinal-type gastric cancers than in
diffuse-type cancers, and the loss of high-dose genes is associated with
hematogenous metastasis. The study is generally well designed. However, there
are several major comments should be addressed:
Major Compulsory Revisions
1) As shown in Table 3, in high-risk genotypes, age is positively correlated with
LOH level. When it comes to low-risk genotypes, age is negatively correlated
with LOH level. This phenomenon should be discussed accordingly?
2)The age and gender should be included in the models of multivariate analysis,
no matter they were significant or not.
Minor Revisions
1) Page8, Paragraph 1, Line 1: “One hundred forty-five” should be “One hundred
and forty-five”.
2) Page8, Paragraph 1, Line 4: “One hundred sixteen” should be “One hundred
and sixteen”.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
assistant