Title: KIF3C inhibits the progression and proliferation of colorectal cancer
Version: 6 Date: 17 Sep 2024
Reviewer:  Kumar S. Bishnupuri
Reviewer's report:
The manuscript still needs edits. Some of examples are as below:
* All 'in vitro' and 'in vivo' should be in italics.
* Abstract: Methods:  Correct this sentence 'KIF3C function expression in CRC cells'
* The tissue was dewaxed after being divided (??)  into 3um slices.
* Correct the structure of this sentence in 'Materials and Methods' section: The NCM-460 normal
colon cancer epithelial cell lines and human colon cancer cell lines (HCT116, SW-480, and
SW-620) were obtained from the (Cell Bank of the Type Culture Collection at the Shanghai
Institute of Cell Biology, Chinese Academy of Sciences).
* Correct the structure of this sentence in 'Materials and Methods' section: The different cell
lines were cultured in RPMI-1640 medium, supplemented with 10% fetal bovine serum (FBS)
(Invitrogen, Carlsbad, CA, USA), and supplemented with  (??) 100 U/mL penicillin and 100µg/
ml streptomycin (SV30010, Hyclone, Cytiva), at 37°C in a 5% CO2 environment.
* 1x10^3 cells were seeded in a 96-well plate and cultured overnight at 37°C. At 24, 48, 72,
and 96 hours, (...better to replace this comma with period) 10 µL of cell counting Kit-8 (CCK-8)
reagent from Beyotime Institute of Biotechnology, Shanghai, China, was added to each well
and incubated for 2 hours.
* Stage I&Stage II - spacing issue.
* Stage III&Stage IV - spacing issue.
* while the SW-620 cells was (either replace 'cells' with 'cell line' or replace 'was' with 'were)
employed to examine the effects of KIF3C overexpression.
* Be consistent with 'was knocked down ' or 'was knockdown' .
* relationship between KIF3C and the PI3K/Akt pathway, with Bax, Bcl-2, and cyclin D-1
proteins associated with apoptosis (Cyclin D1 is not associated with apoptosis??)
* and elevated levels of these proteins in Bax and p53  (??).
* The si-KIF3C was separately transfected into SW-480 and HCT-116 cells (??), while the oe-
KIF3C overexpression (??) was in SW-620 cells.
* Nevertheless, Bcl-2 and the Cyclin D1 cell cycle regulator protein CyclinD1 are of a kind
that is closely linked to the development of tumors (??  -    Bcl-2 is a regulator of apoptosis
and Cyclin D1 is a regulator of cell cycle) - please be careful while mentioning their respective
involvement in apoptosis regulation or cell cycle regulation.
* Conclusion: oncogene KIF3C, whether suppressed or overexpressed, stimulated cell migration
and invasion while suppressing cell apoptosis in CRC - As shown in figure 3, si-KIF3C
(knockdown) inhibited migration and invasion, while in figure  4, oe-KIF3C stimulated
migration and invasion, but  concluding remark says 'whether suppressed or overexpressed,
stimulated cell migration and invasion'.
Overall, although the manuscript includes interesting results, it's painful to see so many errors
and confusing statements  in a second time resubmitted manuscript.
Title: KIF3C inhibits the progression and proliferation of colorectal cancer
Version: 6 Date: 17 Sep 2024
Reviewer:  Kumar S. Bishnupuri
Reviewer's report:
The manuscript still needs edits. Some of examples are as below:
* All 'in vitro' and 'in vivo' should be in italics.
* Abstract: Methods:  Correct this sentence 'KIF3C function expression in CRC cells'
* The tissue was dewaxed after being divided (??)  into 3um slices.
* Correct the structure of this sentence in 'Materials and Methods' section: The NCM-460 normal
colon cancer epithelial cell lines and human colon cancer cell lines (HCT116, SW-480, and
SW-620) were obtained from the (Cell Bank of the Type Culture Collection at the Shanghai
Institute of Cell Biology, Chinese Academy of Sciences).
* Correct the structure of this sentence in 'Materials and Methods' section: The different cell
lines were cultured in RPMI-1640 medium, supplemented with 10% fetal bovine serum (FBS)
(Invitrogen, Carlsbad, CA, USA), and supplemented with  (??) 100 U/mL penicillin and 100µg/
ml streptomycin (SV30010, Hyclone, Cytiva), at 37°C in a 5% CO2 environment.
* 1x10^3 cells were seeded in a 96-well plate and cultured overnight at 37°C. At 24, 48, 72,
and 96 hours, (...better to replace this comma with period) 10 µL of cell counting Kit-8 (CCK-8)
reagent from Beyotime Institute of Biotechnology, Shanghai, China, was added to each well
and incubated for 2 hours.
* Stage I&Stage II - spacing issue.
* Stage III&Stage IV - spacing issue.
* while the SW-620 cells was (either replace 'cells' with 'cell line' or replace 'was' with 'were)
employed to examine the effects of KIF3C overexpression.
* Be consistent with 'was knocked down ' or 'was knockdown' .
* relationship between KIF3C and the PI3K/Akt pathway, with Bax, Bcl-2, and cyclin D-1
proteins associated with apoptosis (Cyclin D1 is not associated with apoptosis??)
* and elevated levels of these proteins in Bax and p53  (??).
* The si-KIF3C was separately transfected into SW-480 and HCT-116 cells (??), while the oe-
KIF3C overexpression (??) was in SW-620 cells.
* Nevertheless, Bcl-2 and the Cyclin D1 cell cycle regulator protein CyclinD1 are of a kind
that is closely linked to the development of tumors (??  -    Bcl-2 is a regulator of apoptosis
and Cyclin D1 is a regulator of cell cycle) - please be careful while mentioning their respective
involvement in apoptosis regulation or cell cycle regulation.
* Conclusion: oncogene KIF3C, whether suppressed or overexpressed, stimulated cell migration
and invasion while suppressing cell apoptosis in CRC - As shown in figure 3, si-KIF3C
(knockdown) inhibited migration and invasion, while in figure  4, oe-KIF3C stimulated
migration and invasion, but  concluding remark says 'whether suppressed or overexpressed,
stimulated cell migration and invasion'.
Overall, although the manuscript includes interesting results, it's painful to see so many errors
and confusing statements  in a second time resubmitted manuscript.
Please review this paper.
user
Title: KIF3C inhibits the progression and proliferation of colorectal cancer
Version: 6 Date: 17 Sep 2024
Reviewer:  Kumar S. Bishnupuri
Reviewer's report:
The manuscript still needs edits. Some of examples are as below:
* All 'in vitro' and 'in vivo' should be in italics.
* Abstract: Methods:  Correct this sentence 'KIF3C function expression in CRC cells'
* The tissue was dewaxed after being divided (??)  into 3um slices.
* Correct the structure of this sentence in 'Materials and Methods' section: The NCM-460 normal
colon cancer epithelial cell lines and human colon cancer cell lines (HCT116, SW-480, and
SW-620) were obtained from the (Cell Bank of the Type Culture Collection at the Shanghai
Institute of Cell Biology, Chinese Academy of Sciences).
* Correct the structure of this sentence in 'Materials and Methods' section: The different cell
lines were cultured in RPMI-1640 medium, supplemented with 10% fetal bovine serum (FBS)
(Invitrogen, Carlsbad, CA, USA), and supplemented with  (??) 100 U/mL penicillin and 100µg/
ml streptomycin (SV30010, Hyclone, Cytiva), at 37°C in a 5% CO2 environment.
* 1x10^3 cells were seeded in a 96-well plate and cultured overnight at 37°C. At 24, 48, 72,
and 96 hours, (...better to replace this comma with period) 10 µL of cell counting Kit-8 (CCK-8)
reagent from Beyotime Institute of Biotechnology, Shanghai, China, was added to each well
and incubated for 2 hours.
* Stage I&Stage II - spacing issue.
* Stage III&Stage IV - spacing issue.
* while the SW-620 cells was (either replace 'cells' with 'cell line' or replace 'was' with 'were)
employed to examine the effects of KIF3C overexpression.
* Be consistent with 'was knocked down ' or 'was knockdown' .
* relationship between KIF3C and the PI3K/Akt pathway, with Bax, Bcl-2, and cyclin D-1
proteins associated with apoptosis (Cyclin D1 is not associated with apoptosis??)
* and elevated levels of these proteins in Bax and p53  (??).
* The si-KIF3C was separately transfected into SW-480 and HCT-116 cells (??), while the oe-
KIF3C overexpression (??) was in SW-620 cells.
* Nevertheless, Bcl-2 and the Cyclin D1 cell cycle regulator protein CyclinD1 are of a kind
that is closely linked to the development of tumors (??  -    Bcl-2 is a regulator of apoptosis
and Cyclin D1 is a regulator of cell cycle) - please be careful while mentioning their respective
involvement in apoptosis regulation or cell cycle regulation.
* Conclusion: oncogene KIF3C, whether suppressed or overexpressed, stimulated cell migration
and invasion while suppressing cell apoptosis in CRC - As shown in figure 3, si-KIF3C
(knockdown) inhibited migration and invasion, while in figure  4, oe-KIF3C stimulated
migration and invasion, but  concluding remark says 'whether suppressed or overexpressed,
stimulated cell migration and invasion'.
Overall, although the manuscript includes interesting results, it's painful to see so many errors
and confusing statements  in a second time resubmitted manuscript.
assistant