Title: Netrin-1-CD146 and S100A9-CD146 are associated with early stage of lymph node
metastasis in colorectal cancer
Version: 1 Date: 23 May 2024
Reviewer's report:
The concept of the study is well described. However, the manuscript should go through
significant clarification to be useful for readers. Some of the points/suggestions regarding this
paper are given below:
1. The authors should ask the pathologist to check the colon tissue histology because some
images did not show representative disease histology. The presentation of such pathology images
may undermine the reliability of the results of subsequent analyses.
2. How did the authors identify the sentinel lymph nodes in this study?
3. For IHC experiments, the antigen retrieval condition should be described in detail.
4. The relationship between CD146 and S100 protein and CRC progression might be novel and
interesting. However, this study did not perform functional analysis using CRC cell lines. The
authors should conduct functional analysis experiments if they claim a novel relationship.
5. In this study, the authors focus on the relationship between lymph node metastasis and
netrin-1/CD146. If so, stratifying the prognostic analysis by the degree of lymph node metastasis
may support the authors' hypothesis more.
Title: Netrin-1-CD146 and S100A9-CD146 are associated with early stage of lymph node
metastasis in colorectal cancer
Version: 1 Date: 23 May 2024
Reviewer's report:
The concept of the study is well described. However, the manuscript should go through
significant clarification to be useful for readers. Some of the points/suggestions regarding this
paper are given below:
1. The authors should ask the pathologist to check the colon tissue histology because some
images did not show representative disease histology. The presentation of such pathology images
may undermine the reliability of the results of subsequent analyses.
2. How did the authors identify the sentinel lymph nodes in this study?
3. For IHC experiments, the antigen retrieval condition should be described in detail.
4. The relationship between CD146 and S100 protein and CRC progression might be novel and
interesting. However, this study did not perform functional analysis using CRC cell lines. The
authors should conduct functional analysis experiments if they claim a novel relationship.
5. In this study, the authors focus on the relationship between lymph node metastasis and
netrin-1/CD146. If so, stratifying the prognostic analysis by the degree of lymph node metastasis
may support the authors' hypothesis more.
Please review this paper.
user
Title: Netrin-1-CD146 and S100A9-CD146 are associated with early stage of lymph node
metastasis in colorectal cancer
Version: 1 Date: 23 May 2024
Reviewer's report:
The concept of the study is well described. However, the manuscript should go through
significant clarification to be useful for readers. Some of the points/suggestions regarding this
paper are given below:
1. The authors should ask the pathologist to check the colon tissue histology because some
images did not show representative disease histology. The presentation of such pathology images
may undermine the reliability of the results of subsequent analyses.
2. How did the authors identify the sentinel lymph nodes in this study?
3. For IHC experiments, the antigen retrieval condition should be described in detail.
4. The relationship between CD146 and S100 protein and CRC progression might be novel and
interesting. However, this study did not perform functional analysis using CRC cell lines. The
authors should conduct functional analysis experiments if they claim a novel relationship.
5. In this study, the authors focus on the relationship between lymph node metastasis and
netrin-1/CD146. If so, stratifying the prognostic analysis by the degree of lymph node metastasis
may support the authors' hypothesis more.
assistant