Reviewer's report
Title:The roles of tumor necrosis factor-alpha in colon tight junction protein
expression and intestinal mucosa structure in a mouse model of acute liver
failure
Version: 4Date:25 May 2009
Reviewer: Salvatore Cuzzocrea
Reviewer's report:
In this paper the authors show that TNF-# affects the structure of the intestinal
mucosa, decreases expression of ZO-1, and affects the morphology of the colon
in a mouse model of acute liver failure, by the use of anti-TNF-# IgG antibody or
anti-TNF-# R1 antibody. The work is well thought-out and well executed, and the
data support the author conclusions, but there are some questions that need to
be addressed:
Major Compulsory Revision
1. TNF-# increases intestinal epithelial tight junction permeability. Moreover it has
been demonstrated that TNF-#-induced increase in Caco-2 cell TJ permeability
was mediated by NF-kappa B activation. What is the role of TNF-# on NF-kappa
B activation in this model?
2. There are data with knockout mice about the role of TNF-# in model of acute
liver failure?
Minor essential revisions
1. The manuscript needs to be carefully revised
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests'
Reviewer's report
Title:The roles of tumor necrosis factor-alpha in colon tight junction protein
expression and intestinal mucosa structure in a mouse model of acute liver
failure
Version: 4Date:25 May 2009
Reviewer: Salvatore Cuzzocrea
Reviewer's report:
In this paper the authors show that TNF-# affects the structure of the intestinal
mucosa, decreases expression of ZO-1, and affects the morphology of the colon
in a mouse model of acute liver failure, by the use of anti-TNF-# IgG antibody or
anti-TNF-# R1 antibody. The work is well thought-out and well executed, and the
data support the author conclusions, but there are some questions that need to
be addressed:
Major Compulsory Revision
1. TNF-# increases intestinal epithelial tight junction permeability. Moreover it has
been demonstrated that TNF-#-induced increase in Caco-2 cell TJ permeability
was mediated by NF-kappa B activation. What is the role of TNF-# on NF-kappa
B activation in this model?
2. There are data with knockout mice about the role of TNF-# in model of acute
liver failure?
Minor essential revisions
1. The manuscript needs to be carefully revised
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests'
Please review this paper.
user
Reviewer's report
Title:The roles of tumor necrosis factor-alpha in colon tight junction protein
expression and intestinal mucosa structure in a mouse model of acute liver
failure
Version: 4Date:25 May 2009
Reviewer: Salvatore Cuzzocrea
Reviewer's report:
In this paper the authors show that TNF-# affects the structure of the intestinal
mucosa, decreases expression of ZO-1, and affects the morphology of the colon
in a mouse model of acute liver failure, by the use of anti-TNF-# IgG antibody or
anti-TNF-# R1 antibody. The work is well thought-out and well executed, and the
data support the author conclusions, but there are some questions that need to
be addressed:
Major Compulsory Revision
1. TNF-# increases intestinal epithelial tight junction permeability. Moreover it has
been demonstrated that TNF-#-induced increase in Caco-2 cell TJ permeability
was mediated by NF-kappa B activation. What is the role of TNF-# on NF-kappa
B activation in this model?
2. There are data with knockout mice about the role of TNF-# in model of acute
liver failure?
Minor essential revisions
1. The manuscript needs to be carefully revised
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests'
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