Reviewer's report
Title:Association of the -511C/-31T haplotype of IL-1beta and genotypes of
Helicobacter pylori-vacA with chronic gastritis and gastric ulcer
Version: 1Date:24 May 2010
Reviewer: Mitsushige Sugimoto
Reviewer's report:
General:
The authors investigated the effects of bacterial factors of H. pylori (vacA) and
host genetic factors of IL-1B on the development of gastritis and peptic ulcer.
Authors showed that the host IL-1B polymorphisms could play a role in
determining kinds of H. pylori-related diseases in Mexican population. Previously,
although there were many studies to investigate association of diseases
development and host genetic factors of IL-1B, results were controversial, as
suggested by authors. Therefore, this study may have potential to evaluate
evidences of this field.
Major comments:
1. Most important thing of this study is to have sufficient sample number to
evaluate significances. Is it enough? Please analyze sample power and add this
information in revised paper.
2. It is difficult to consider risks of H. pylori infection-related disease development
by only one parameter, such as IL-1B and vacA genotype. How about combined
analysis of IL-1B and vacA genotype?
3. Authors introduced that two previous studies have investigated the
IL-1B-31C>T polymorphism in Mexican population. Were there differences in
prevalence of IL-1B genotypes (control and patients) between previous studies
and this study? If so, it may suggest sampling error.
4. As authors suggested, the association of IL-1B polymorphism on development
of diseases controversial in the world. Therefore, it will be required to measure
IL-1beta level among different genotype groups in Mexican population.
5. Authors explained that the lack of a significant association between the vacA
genotypes with gastric ulcer when compared to chronic gastritis could be
explained by the similarity between the genotypic frequencies in both gastritis
and peptic ulcer. In East-Asian population, most of strains were cagA-positive,
vacA s1 and m1 genotypes. If so, above explanation will be acceptable.
However, prevalence of s1 and m1 were 82-88% and 50-58%. Therefore, I think
there is variation. One of causes may be sampling error, especially in gastric
ulcer, and loss of sample number.
Minor comments:
1. There are many typographical error in this version. Please check carefully.
2. How about mixed infection of H. pylori? Previous Mexican study had high rate
of mixed infection.
3. Do you have data of grade of gastric mucosal atrophy?
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Reviewer's report
Title:Association of the -511C/-31T haplotype of IL-1beta and genotypes of
Helicobacter pylori-vacA with chronic gastritis and gastric ulcer
Version: 1Date:24 May 2010
Reviewer: Mitsushige Sugimoto
Reviewer's report:
General:
The authors investigated the effects of bacterial factors of H. pylori (vacA) and
host genetic factors of IL-1B on the development of gastritis and peptic ulcer.
Authors showed that the host IL-1B polymorphisms could play a role in
determining kinds of H. pylori-related diseases in Mexican population. Previously,
although there were many studies to investigate association of diseases
development and host genetic factors of IL-1B, results were controversial, as
suggested by authors. Therefore, this study may have potential to evaluate
evidences of this field.
Major comments:
1. Most important thing of this study is to have sufficient sample number to
evaluate significances. Is it enough? Please analyze sample power and add this
information in revised paper.
2. It is difficult to consider risks of H. pylori infection-related disease development
by only one parameter, such as IL-1B and vacA genotype. How about combined
analysis of IL-1B and vacA genotype?
3. Authors introduced that two previous studies have investigated the
IL-1B-31C>T polymorphism in Mexican population. Were there differences in
prevalence of IL-1B genotypes (control and patients) between previous studies
and this study? If so, it may suggest sampling error.
4. As authors suggested, the association of IL-1B polymorphism on development
of diseases controversial in the world. Therefore, it will be required to measure
IL-1beta level among different genotype groups in Mexican population.
5. Authors explained that the lack of a significant association between the vacA
genotypes with gastric ulcer when compared to chronic gastritis could be
explained by the similarity between the genotypic frequencies in both gastritis
and peptic ulcer. In East-Asian population, most of strains were cagA-positive,
vacA s1 and m1 genotypes. If so, above explanation will be acceptable.
However, prevalence of s1 and m1 were 82-88% and 50-58%. Therefore, I think
there is variation. One of causes may be sampling error, especially in gastric
ulcer, and loss of sample number.
Minor comments:
1. There are many typographical error in this version. Please check carefully.
2. How about mixed infection of H. pylori? Previous Mexican study had high rate
of mixed infection.
3. Do you have data of grade of gastric mucosal atrophy?
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Please review this paper.
user
Reviewer's report
Title:Association of the -511C/-31T haplotype of IL-1beta and genotypes of
Helicobacter pylori-vacA with chronic gastritis and gastric ulcer
Version: 1Date:24 May 2010
Reviewer: Mitsushige Sugimoto
Reviewer's report:
General:
The authors investigated the effects of bacterial factors of H. pylori (vacA) and
host genetic factors of IL-1B on the development of gastritis and peptic ulcer.
Authors showed that the host IL-1B polymorphisms could play a role in
determining kinds of H. pylori-related diseases in Mexican population. Previously,
although there were many studies to investigate association of diseases
development and host genetic factors of IL-1B, results were controversial, as
suggested by authors. Therefore, this study may have potential to evaluate
evidences of this field.
Major comments:
1. Most important thing of this study is to have sufficient sample number to
evaluate significances. Is it enough? Please analyze sample power and add this
information in revised paper.
2. It is difficult to consider risks of H. pylori infection-related disease development
by only one parameter, such as IL-1B and vacA genotype. How about combined
analysis of IL-1B and vacA genotype?
3. Authors introduced that two previous studies have investigated the
IL-1B-31C>T polymorphism in Mexican population. Were there differences in
prevalence of IL-1B genotypes (control and patients) between previous studies
and this study? If so, it may suggest sampling error.
4. As authors suggested, the association of IL-1B polymorphism on development
of diseases controversial in the world. Therefore, it will be required to measure
IL-1beta level among different genotype groups in Mexican population.
5. Authors explained that the lack of a significant association between the vacA
genotypes with gastric ulcer when compared to chronic gastritis could be
explained by the similarity between the genotypic frequencies in both gastritis
and peptic ulcer. In East-Asian population, most of strains were cagA-positive,
vacA s1 and m1 genotypes. If so, above explanation will be acceptable.
However, prevalence of s1 and m1 were 82-88% and 50-58%. Therefore, I think
there is variation. One of causes may be sampling error, especially in gastric
ulcer, and loss of sample number.
Minor comments:
1. There are many typographical error in this version. Please check carefully.
2. How about mixed infection of H. pylori? Previous Mexican study had high rate
of mixed infection.
3. Do you have data of grade of gastric mucosal atrophy?
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
assistant