Reviewer's report
Title:Impact of Gastroesophageal Reflux on MicroRNA Expression, Location
and Function
Version: 1Date:24 October 2012
Reviewer: Andrew Feber
Reviewer's report:
The manuscript by Smith et al., "Impact of Gastroesophageal Reflux on
MicroRNA Expression, Location and Function". The authors report the
expression of a limited number of microRNAs in a small cohort of mucosa
samples from oesophageal reflux (n=10) and control patients. Through the use of
rt-pcr they show a significant difference in the expression of 3 miRNAs. They
subsequently show that over expression of these miRNAs induces decreased
proliferation and increased apoptosis. They also cells over expressing the miRNA
to be located in the basal epithelium.
The statistics appear suitable.
The data presented is of some interest, and for the first time shows the increased
expression of miRNAs in non-diseased esophageal mucossa. Although all the
analysed miRNAs have previously been shown to be unregulated in barretts
esophagus. However, the analysis has been carried out in only a small sample
cohort and further validation in an enlarged set would be highly desirable,
although not a pre-requisite for publication.
Major Compulsory Revisions
Minor Essential Revisions
1)The author need to address the figures and figure legends. At present the
figure labels not not match those in the text. Further more the the figure
particularly the ISH figures are unclear and fuzzy, higher quality images are
needed.
2) A more in depth assessment of the expression of miRNA target genes would
also be desirable.
3) Show effect on the expression of CK8, BMP4 and CK14 upon transfection of
miRNA
Discretionary Revisions
1)The mirnas identified have already been shown to be over expressed in
Barrett's esophagus, and a further detailed analysis of the role of these
microRNAs in the from reflux to barretts to malignant disease would be desirable.
2) is there any association between miRNA expression and progression to
barretts esophagus.
Level of interest: An article of limited interest
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
Declaration of competing interests:
'I declare that I have no competing interests
Reviewer's report
Title:Impact of Gastroesophageal Reflux on MicroRNA Expression, Location
and Function
Version: 1Date:24 October 2012
Reviewer: Andrew Feber
Reviewer's report:
The manuscript by Smith et al., "Impact of Gastroesophageal Reflux on
MicroRNA Expression, Location and Function". The authors report the
expression of a limited number of microRNAs in a small cohort of mucosa
samples from oesophageal reflux (n=10) and control patients. Through the use of
rt-pcr they show a significant difference in the expression of 3 miRNAs. They
subsequently show that over expression of these miRNAs induces decreased
proliferation and increased apoptosis. They also cells over expressing the miRNA
to be located in the basal epithelium.
The statistics appear suitable.
The data presented is of some interest, and for the first time shows the increased
expression of miRNAs in non-diseased esophageal mucossa. Although all the
analysed miRNAs have previously been shown to be unregulated in barretts
esophagus. However, the analysis has been carried out in only a small sample
cohort and further validation in an enlarged set would be highly desirable,
although not a pre-requisite for publication.
Major Compulsory Revisions
Minor Essential Revisions
1)The author need to address the figures and figure legends. At present the
figure labels not not match those in the text. Further more the the figure
particularly the ISH figures are unclear and fuzzy, higher quality images are
needed.
2) A more in depth assessment of the expression of miRNA target genes would
also be desirable.
3) Show effect on the expression of CK8, BMP4 and CK14 upon transfection of
miRNA
Discretionary Revisions
1)The mirnas identified have already been shown to be over expressed in
Barrett's esophagus, and a further detailed analysis of the role of these
microRNAs in the from reflux to barretts to malignant disease would be desirable.
2) is there any association between miRNA expression and progression to
barretts esophagus.
Level of interest: An article of limited interest
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
Declaration of competing interests:
'I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Impact of Gastroesophageal Reflux on MicroRNA Expression, Location
and Function
Version: 1Date:24 October 2012
Reviewer: Andrew Feber
Reviewer's report:
The manuscript by Smith et al., "Impact of Gastroesophageal Reflux on
MicroRNA Expression, Location and Function". The authors report the
expression of a limited number of microRNAs in a small cohort of mucosa
samples from oesophageal reflux (n=10) and control patients. Through the use of
rt-pcr they show a significant difference in the expression of 3 miRNAs. They
subsequently show that over expression of these miRNAs induces decreased
proliferation and increased apoptosis. They also cells over expressing the miRNA
to be located in the basal epithelium.
The statistics appear suitable.
The data presented is of some interest, and for the first time shows the increased
expression of miRNAs in non-diseased esophageal mucossa. Although all the
analysed miRNAs have previously been shown to be unregulated in barretts
esophagus. However, the analysis has been carried out in only a small sample
cohort and further validation in an enlarged set would be highly desirable,
although not a pre-requisite for publication.
Major Compulsory Revisions
Minor Essential Revisions
1)The author need to address the figures and figure legends. At present the
figure labels not not match those in the text. Further more the the figure
particularly the ISH figures are unclear and fuzzy, higher quality images are
needed.
2) A more in depth assessment of the expression of miRNA target genes would
also be desirable.
3) Show effect on the expression of CK8, BMP4 and CK14 upon transfection of
miRNA
Discretionary Revisions
1)The mirnas identified have already been shown to be over expressed in
Barrett's esophagus, and a further detailed analysis of the role of these
microRNAs in the from reflux to barretts to malignant disease would be desirable.
2) is there any association between miRNA expression and progression to
barretts esophagus.
Level of interest: An article of limited interest
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
Declaration of competing interests:
'I declare that I have no competing interests
assistant