Reviewer's report
Title:Weak Up-regulation of Serum Response Factor in Gastric Ulcers in
Patients with Co-morbidities is Associated with Increased Risk of Recurrent
Bleeding
Version: 1Date:16 November 2010
Reviewer: Peter C Konturek
Reviewer's report:
Manuscripts by Cheng et al entitled “Weak up-regulation of serum response
factor in gastric ulcers in patients with co-morbidities is associated with increased
risk of recurrent bleeding” determined the association between
immunohistochemical expression of SRF in gastric ulcers and risk of recurrent
bleeding. The multivariate logistic regression confirmed that co-morbidities and
weak SRF-expression were two independent factors of recurrent gastric ulcer
bleeding.
Major comments:
Both NSAIDs and H.pylori, thr most important damaging factors of gastric
mucosa had no effect on SRF expression. Since SRF plays a central role in ulcer
healing, how this phenomenon could be explained? The authors should discuss
this issue in context of literature.
What was the positive control for immunohistochemical staining for SRF?
Authors should include the positive SRF-immunostaining control in the
manuscript.
SRF is an important component of ulcer healing which promotes migration and
proliferation of gastric epithelial and smooth muscle cells. Authors should analyse
the changes in proliferation index (PCNA or Ki-67) at the ulcer edge in
dependence of SRF expression.
Decrease in SRF expression inhibits angiogenesis driven by VEGF, which plays
a crucial role in ulcer healing. It would be of importance to investigate this aspect
of ulcer healing. Did patients with low SRF-expression had decreased VEGF
expression?
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Reviewer's report
Title:Weak Up-regulation of Serum Response Factor in Gastric Ulcers in
Patients with Co-morbidities is Associated with Increased Risk of Recurrent
Bleeding
Version: 1Date:16 November 2010
Reviewer: Peter C Konturek
Reviewer's report:
Manuscripts by Cheng et al entitled “Weak up-regulation of serum response
factor in gastric ulcers in patients with co-morbidities is associated with increased
risk of recurrent bleeding” determined the association between
immunohistochemical expression of SRF in gastric ulcers and risk of recurrent
bleeding. The multivariate logistic regression confirmed that co-morbidities and
weak SRF-expression were two independent factors of recurrent gastric ulcer
bleeding.
Major comments:
Both NSAIDs and H.pylori, thr most important damaging factors of gastric
mucosa had no effect on SRF expression. Since SRF plays a central role in ulcer
healing, how this phenomenon could be explained? The authors should discuss
this issue in context of literature.
What was the positive control for immunohistochemical staining for SRF?
Authors should include the positive SRF-immunostaining control in the
manuscript.
SRF is an important component of ulcer healing which promotes migration and
proliferation of gastric epithelial and smooth muscle cells. Authors should analyse
the changes in proliferation index (PCNA or Ki-67) at the ulcer edge in
dependence of SRF expression.
Decrease in SRF expression inhibits angiogenesis driven by VEGF, which plays
a crucial role in ulcer healing. It would be of importance to investigate this aspect
of ulcer healing. Did patients with low SRF-expression had decreased VEGF
expression?
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Please review this paper.
user
Reviewer's report
Title:Weak Up-regulation of Serum Response Factor in Gastric Ulcers in
Patients with Co-morbidities is Associated with Increased Risk of Recurrent
Bleeding
Version: 1Date:16 November 2010
Reviewer: Peter C Konturek
Reviewer's report:
Manuscripts by Cheng et al entitled “Weak up-regulation of serum response
factor in gastric ulcers in patients with co-morbidities is associated with increased
risk of recurrent bleeding” determined the association between
immunohistochemical expression of SRF in gastric ulcers and risk of recurrent
bleeding. The multivariate logistic regression confirmed that co-morbidities and
weak SRF-expression were two independent factors of recurrent gastric ulcer
bleeding.
Major comments:
Both NSAIDs and H.pylori, thr most important damaging factors of gastric
mucosa had no effect on SRF expression. Since SRF plays a central role in ulcer
healing, how this phenomenon could be explained? The authors should discuss
this issue in context of literature.
What was the positive control for immunohistochemical staining for SRF?
Authors should include the positive SRF-immunostaining control in the
manuscript.
SRF is an important component of ulcer healing which promotes migration and
proliferation of gastric epithelial and smooth muscle cells. Authors should analyse
the changes in proliferation index (PCNA or Ki-67) at the ulcer edge in
dependence of SRF expression.
Decrease in SRF expression inhibits angiogenesis driven by VEGF, which plays
a crucial role in ulcer healing. It would be of importance to investigate this aspect
of ulcer healing. Did patients with low SRF-expression had decreased VEGF
expression?
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
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