Reviewer's report
Title:A pH-sensitive gel added improves the stability of microemulsion for
orientating removal of colonic ammonia in gastrointestinal tract
Version: 2Date:21 September 2010
Reviewer: BEOM-JIN LEE
Reviewer's report:
No page numbering is very difficult for me to review the manuscript!!
The microemulsion-based gels for removal of ammonia in colon were prepared to
prevent hepatic encephalopathy. Colonic ammonia, appearances and pH values
of gastrointestinal fluid were detected. However, the system is poorly designed
and NOT scientifically clear. The w/o microemulsion formulation design should
be clearly described.
The goal is very unclear. The background should be clearly described why the
microemulsion is useful to remove ammonia in colon. The references are also
needed.
More importantly, there is no information of o/w ME formulation and preparation
method in EXP part. How do you prove the type of ME?
The alginate is stable in low pH but swells in high pH solution. However, it’s hard
to say that the alginate system containing w/o microemulsion is pH sensitive and
stable in GI system.
The alginate gel is widely used for controlled release drug delivery but not a good
candidate of adsorption of ammonia. What is the adsorption principle of w/o
ME-RA or MBD-RA?
Show the physical stability of w/o ME when added to the alginate aqueous gel.
Explain the detailed route of administration in rats (Group I, II, III).
There are many types of gels commercially available. Explain the specific
reasons to select the alginate gels.
How can you determine the concentration of ammonia?
What is the meaning of “Proper proportion of both preparation~~” Explain the
“Proper”!
Abstract part: Explain the unclear text “In simulative gastrointestinal process
MBGs-RA could [0]keep more stable and release emulsion(ME-RA) in colon
fluid”
What is the MBGs-RA[0]?
How do you measure the stability and release emulsion (ME-RA) from MBE-RA
in GI tract?
Is the pH changes an index of stability?
In general, the texts are poorly written and unclear. There are many errors and
mistakes in texts and sentences to be improved English. I recommend editorial
service of the manuscript.
Experiemetal part : “The clearance rates of artificial colon ammonia were
compared in the simulative gastrointestinal transit time and pH environment,
performed as described before.”
“After stained by sudan #, the morphous of the collected content in each group
was observed under microscope”
Level of interest: An article of limited interest
Quality of written English: Not suitable for publication unless extensively edited
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Reviewer's report
Title:A pH-sensitive gel added improves the stability of microemulsion for
orientating removal of colonic ammonia in gastrointestinal tract
Version: 2Date:21 September 2010
Reviewer: BEOM-JIN LEE
Reviewer's report:
No page numbering is very difficult for me to review the manuscript!!
The microemulsion-based gels for removal of ammonia in colon were prepared to
prevent hepatic encephalopathy. Colonic ammonia, appearances and pH values
of gastrointestinal fluid were detected. However, the system is poorly designed
and NOT scientifically clear. The w/o microemulsion formulation design should
be clearly described.
The goal is very unclear. The background should be clearly described why the
microemulsion is useful to remove ammonia in colon. The references are also
needed.
More importantly, there is no information of o/w ME formulation and preparation
method in EXP part. How do you prove the type of ME?
The alginate is stable in low pH but swells in high pH solution. However, it’s hard
to say that the alginate system containing w/o microemulsion is pH sensitive and
stable in GI system.
The alginate gel is widely used for controlled release drug delivery but not a good
candidate of adsorption of ammonia. What is the adsorption principle of w/o
ME-RA or MBD-RA?
Show the physical stability of w/o ME when added to the alginate aqueous gel.
Explain the detailed route of administration in rats (Group I, II, III).
There are many types of gels commercially available. Explain the specific
reasons to select the alginate gels.
How can you determine the concentration of ammonia?
What is the meaning of “Proper proportion of both preparation~~” Explain the
“Proper”!
Abstract part: Explain the unclear text “In simulative gastrointestinal process
MBGs-RA could [0]keep more stable and release emulsion(ME-RA) in colon
fluid”
What is the MBGs-RA[0]?
How do you measure the stability and release emulsion (ME-RA) from MBE-RA
in GI tract?
Is the pH changes an index of stability?
In general, the texts are poorly written and unclear. There are many errors and
mistakes in texts and sentences to be improved English. I recommend editorial
service of the manuscript.
Experiemetal part : “The clearance rates of artificial colon ammonia were
compared in the simulative gastrointestinal transit time and pH environment,
performed as described before.”
“After stained by sudan #, the morphous of the collected content in each group
was observed under microscope”
Level of interest: An article of limited interest
Quality of written English: Not suitable for publication unless extensively edited
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Please review this paper.
user
Reviewer's report
Title:A pH-sensitive gel added improves the stability of microemulsion for
orientating removal of colonic ammonia in gastrointestinal tract
Version: 2Date:21 September 2010
Reviewer: BEOM-JIN LEE
Reviewer's report:
No page numbering is very difficult for me to review the manuscript!!
The microemulsion-based gels for removal of ammonia in colon were prepared to
prevent hepatic encephalopathy. Colonic ammonia, appearances and pH values
of gastrointestinal fluid were detected. However, the system is poorly designed
and NOT scientifically clear. The w/o microemulsion formulation design should
be clearly described.
The goal is very unclear. The background should be clearly described why the
microemulsion is useful to remove ammonia in colon. The references are also
needed.
More importantly, there is no information of o/w ME formulation and preparation
method in EXP part. How do you prove the type of ME?
The alginate is stable in low pH but swells in high pH solution. However, it’s hard
to say that the alginate system containing w/o microemulsion is pH sensitive and
stable in GI system.
The alginate gel is widely used for controlled release drug delivery but not a good
candidate of adsorption of ammonia. What is the adsorption principle of w/o
ME-RA or MBD-RA?
Show the physical stability of w/o ME when added to the alginate aqueous gel.
Explain the detailed route of administration in rats (Group I, II, III).
There are many types of gels commercially available. Explain the specific
reasons to select the alginate gels.
How can you determine the concentration of ammonia?
What is the meaning of “Proper proportion of both preparation~~” Explain the
“Proper”!
Abstract part: Explain the unclear text “In simulative gastrointestinal process
MBGs-RA could [0]keep more stable and release emulsion(ME-RA) in colon
fluid”
What is the MBGs-RA[0]?
How do you measure the stability and release emulsion (ME-RA) from MBE-RA
in GI tract?
Is the pH changes an index of stability?
In general, the texts are poorly written and unclear. There are many errors and
mistakes in texts and sentences to be improved English. I recommend editorial
service of the manuscript.
Experiemetal part : “The clearance rates of artificial colon ammonia were
compared in the simulative gastrointestinal transit time and pH environment,
performed as described before.”
“After stained by sudan #, the morphous of the collected content in each group
was observed under microscope”
Level of interest: An article of limited interest
Quality of written English: Not suitable for publication unless extensively edited
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
assistant