Title: Investigation of the Mechanism by which miR-223-3p Inhibits Reflux Esophagitis
through Targeting the NLRP3 Inflammasome
Version: 3 Date: 14 Feb 2025
Reviewer's report:
The manuscript by Shuying Lin et al. describes the protective role of miR-223-3p against reflux
esophagitis. Overexpression of miR-223-3p markedly inhibited NLRP3 expression, reduced the
release of inflammatory factors, decreased cell apoptosis, promoted cell cycle progression, and
enhanced cell viability. However, their conclusions are based solely on experiments conducted
using a single cell line (HET-1A). While the regulatory mechanisms of miR-223-3p play a key
role in reducing inflammation and cellular damage in reflux esophagitis by targeting the NLRP3
inflammasome, these mechanisms need to be further explored. I recommend that the authors
include experiments utilizing mouse models or human samples that focus on reflux esophagitis.
Title: Investigation of the Mechanism by which miR-223-3p Inhibits Reflux Esophagitis
through Targeting the NLRP3 Inflammasome
Version: 3 Date: 14 Feb 2025
Reviewer's report:
The manuscript by Shuying Lin et al. describes the protective role of miR-223-3p against reflux
esophagitis. Overexpression of miR-223-3p markedly inhibited NLRP3 expression, reduced the
release of inflammatory factors, decreased cell apoptosis, promoted cell cycle progression, and
enhanced cell viability. However, their conclusions are based solely on experiments conducted
using a single cell line (HET-1A). While the regulatory mechanisms of miR-223-3p play a key
role in reducing inflammation and cellular damage in reflux esophagitis by targeting the NLRP3
inflammasome, these mechanisms need to be further explored. I recommend that the authors
include experiments utilizing mouse models or human samples that focus on reflux esophagitis.
Please review this paper.
user
Title: Investigation of the Mechanism by which miR-223-3p Inhibits Reflux Esophagitis
through Targeting the NLRP3 Inflammasome
Version: 3 Date: 14 Feb 2025
Reviewer's report:
The manuscript by Shuying Lin et al. describes the protective role of miR-223-3p against reflux
esophagitis. Overexpression of miR-223-3p markedly inhibited NLRP3 expression, reduced the
release of inflammatory factors, decreased cell apoptosis, promoted cell cycle progression, and
enhanced cell viability. However, their conclusions are based solely on experiments conducted
using a single cell line (HET-1A). While the regulatory mechanisms of miR-223-3p play a key
role in reducing inflammation and cellular damage in reflux esophagitis by targeting the NLRP3
inflammasome, these mechanisms need to be further explored. I recommend that the authors
include experiments utilizing mouse models or human samples that focus on reflux esophagitis.
assistant