Reviewer's report
Title:Differential hepatotoxicity of dietary and DNL-derived palmitate in the
methionine-choline-deficient model of steatohepatitis
Version:2Date:22 March 2015
Reviewer: Julia Kälsch
Reviewer's report:
Review of BMC Gastroenterology Article titled “Differential hepatotoxicity of
dietary and DNL-derived palmitate in the methionine-choline-deficient model of
staetohepatitis” by Andrew A. Pierce, Michael K. Pickens, Kevin Siao, James P.
Grenert and Jacquelyn J. Maher
The manuscript by Andrew A. Pierce et al. performed an analysis of liver injury
induced by custom methionine-choline-deficient (MCD) diet with 4 different
macronutrient combinations in mice. The objective of the study was to determine
whether dietary palmitate exerts the same toxicity as carbohydrate-derived
palmitate.
Adult male mice were fed for 21 days with one of different custom
methionine-choline-sufficient (MCS) or MCD diet formulas. The custom-made
formulas contained different components of carbohydrates and fats (e.g. starch
or sucrose, oleate or palmetate). After the mice were killed, serum alanine
aminotransferase (ALT) was measured, triglyceride and fatty acid analysis was
performed by extracting lipids from fresh liver tissue, furthermore, gene
expression was evaluated by extraction of total RNA from liver tissue. Results
were compared by analysis of variance with Tukey post-hoc testing.
The manuscript is well written and the study design was appropriate for the given
aims. The authors managed to sum up interesting and important results mainly
indicating that dietary and DNL palmitate have different effects on liver cells in
mice. They also managed the challenge to visualize their results, clearly giving
the reader an impression of the most important findings.
Major comments:
- No major comments
Minor comments:
- The authors should also measure aspartate amino transferase (AST), not only
ALT, as well as gamma-glutamyl transferase (GGT), to determine the whole
spectrum of liver damage.
- Performance of M-30 and M-65 cell death assay would be another tool to
evaluate the difference in liver injury.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:Differential hepatotoxicity of dietary and DNL-derived palmitate in the
methionine-choline-deficient model of steatohepatitis
Version:2Date:22 March 2015
Reviewer: Julia Kälsch
Reviewer's report:
Review of BMC Gastroenterology Article titled “Differential hepatotoxicity of
dietary and DNL-derived palmitate in the methionine-choline-deficient model of
staetohepatitis” by Andrew A. Pierce, Michael K. Pickens, Kevin Siao, James P.
Grenert and Jacquelyn J. Maher
The manuscript by Andrew A. Pierce et al. performed an analysis of liver injury
induced by custom methionine-choline-deficient (MCD) diet with 4 different
macronutrient combinations in mice. The objective of the study was to determine
whether dietary palmitate exerts the same toxicity as carbohydrate-derived
palmitate.
Adult male mice were fed for 21 days with one of different custom
methionine-choline-sufficient (MCS) or MCD diet formulas. The custom-made
formulas contained different components of carbohydrates and fats (e.g. starch
or sucrose, oleate or palmetate). After the mice were killed, serum alanine
aminotransferase (ALT) was measured, triglyceride and fatty acid analysis was
performed by extracting lipids from fresh liver tissue, furthermore, gene
expression was evaluated by extraction of total RNA from liver tissue. Results
were compared by analysis of variance with Tukey post-hoc testing.
The manuscript is well written and the study design was appropriate for the given
aims. The authors managed to sum up interesting and important results mainly
indicating that dietary and DNL palmitate have different effects on liver cells in
mice. They also managed the challenge to visualize their results, clearly giving
the reader an impression of the most important findings.
Major comments:
- No major comments
Minor comments:
- The authors should also measure aspartate amino transferase (AST), not only
ALT, as well as gamma-glutamyl transferase (GGT), to determine the whole
spectrum of liver damage.
- Performance of M-30 and M-65 cell death assay would be another tool to
evaluate the difference in liver injury.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:Differential hepatotoxicity of dietary and DNL-derived palmitate in the
methionine-choline-deficient model of steatohepatitis
Version:2Date:22 March 2015
Reviewer: Julia Kälsch
Reviewer's report:
Review of BMC Gastroenterology Article titled “Differential hepatotoxicity of
dietary and DNL-derived palmitate in the methionine-choline-deficient model of
staetohepatitis” by Andrew A. Pierce, Michael K. Pickens, Kevin Siao, James P.
Grenert and Jacquelyn J. Maher
The manuscript by Andrew A. Pierce et al. performed an analysis of liver injury
induced by custom methionine-choline-deficient (MCD) diet with 4 different
macronutrient combinations in mice. The objective of the study was to determine
whether dietary palmitate exerts the same toxicity as carbohydrate-derived
palmitate.
Adult male mice were fed for 21 days with one of different custom
methionine-choline-sufficient (MCS) or MCD diet formulas. The custom-made
formulas contained different components of carbohydrates and fats (e.g. starch
or sucrose, oleate or palmetate). After the mice were killed, serum alanine
aminotransferase (ALT) was measured, triglyceride and fatty acid analysis was
performed by extracting lipids from fresh liver tissue, furthermore, gene
expression was evaluated by extraction of total RNA from liver tissue. Results
were compared by analysis of variance with Tukey post-hoc testing.
The manuscript is well written and the study design was appropriate for the given
aims. The authors managed to sum up interesting and important results mainly
indicating that dietary and DNL palmitate have different effects on liver cells in
mice. They also managed the challenge to visualize their results, clearly giving
the reader an impression of the most important findings.
Major comments:
- No major comments
Minor comments:
- The authors should also measure aspartate amino transferase (AST), not only
ALT, as well as gamma-glutamyl transferase (GGT), to determine the whole
spectrum of liver damage.
- Performance of M-30 and M-65 cell death assay would be another tool to
evaluate the difference in liver injury.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests.
assistant