Reviewer's report
Title:Adhesion GPCRs are widely expressed throughout the subsections of the
gastrointestinal tract
Version: 2Date:27 June 2012
Reviewer: Hsi-Hsien Lin
Reviewer's report:
The manuscript described a mRNA profiling analysis of 30 adhesion-GPCRs in
the rat gastrointestinal tract. The relative expression levels of 30
adhesion-GPCRs were analyzed by qRT-PCR analysis in the rat GI tract divided
into many different sections. The authors identified three categories of
expression patterns in the analysis: ubiquitous, widespread and restricted. The
authors concluded that the adhesion-GPCRs might play an important role in the
function of GI tract and considered them as potential drug targets.
Extended from previous studies on the expression patterns of adhesion-GPCRs
by the same group of authors, this manuscript is an attempt to look at the
detailed expression profiles of adhesion-GPCRs in the GI tract. Thus, the GI tract
was divided into many different segments. The data suggested that
adhesion-GPCR members are expressed ubiquitously, widely, or restricted in the
GI tract. The expression patterns of individual adhesion-GPCR hence implicate
the potential importance in the GI tract function.
The data presented in the manuscript are overall well-controlled and provided a
satisfactory explanation. The information might be useful for the later
physiological and pathological study of GI tract. However, caution should be
taken as the expression data are purely at the transcript levels. There are a few
minor points that need to be clarified better.
Minor Essential Revisions:
1. The primers used in the study are located in the 7TM region. However, it is
well known that many adhesion-GPCR genes undergo extensive alternative
splicing and generate multiple transcripts that include soluble isoforms. Hence,
the primers used might not cover the entire transcript populations. This should be
somehow discussed in the paper. Also, it might be a good idea to list the
expected size of the PCR product in the Table.
2. It will be beneficial to summarize the data in a Table listing the relative
expression levels of all 30 adhesion-GPCR members based on the phylogenetic
groups.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Adhesion GPCRs are widely expressed throughout the subsections of the
gastrointestinal tract
Version: 2Date:27 June 2012
Reviewer: Hsi-Hsien Lin
Reviewer's report:
The manuscript described a mRNA profiling analysis of 30 adhesion-GPCRs in
the rat gastrointestinal tract. The relative expression levels of 30
adhesion-GPCRs were analyzed by qRT-PCR analysis in the rat GI tract divided
into many different sections. The authors identified three categories of
expression patterns in the analysis: ubiquitous, widespread and restricted. The
authors concluded that the adhesion-GPCRs might play an important role in the
function of GI tract and considered them as potential drug targets.
Extended from previous studies on the expression patterns of adhesion-GPCRs
by the same group of authors, this manuscript is an attempt to look at the
detailed expression profiles of adhesion-GPCRs in the GI tract. Thus, the GI tract
was divided into many different segments. The data suggested that
adhesion-GPCR members are expressed ubiquitously, widely, or restricted in the
GI tract. The expression patterns of individual adhesion-GPCR hence implicate
the potential importance in the GI tract function.
The data presented in the manuscript are overall well-controlled and provided a
satisfactory explanation. The information might be useful for the later
physiological and pathological study of GI tract. However, caution should be
taken as the expression data are purely at the transcript levels. There are a few
minor points that need to be clarified better.
Minor Essential Revisions:
1. The primers used in the study are located in the 7TM region. However, it is
well known that many adhesion-GPCR genes undergo extensive alternative
splicing and generate multiple transcripts that include soluble isoforms. Hence,
the primers used might not cover the entire transcript populations. This should be
somehow discussed in the paper. Also, it might be a good idea to list the
expected size of the PCR product in the Table.
2. It will be beneficial to summarize the data in a Table listing the relative
expression levels of all 30 adhesion-GPCR members based on the phylogenetic
groups.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Adhesion GPCRs are widely expressed throughout the subsections of the
gastrointestinal tract
Version: 2Date:27 June 2012
Reviewer: Hsi-Hsien Lin
Reviewer's report:
The manuscript described a mRNA profiling analysis of 30 adhesion-GPCRs in
the rat gastrointestinal tract. The relative expression levels of 30
adhesion-GPCRs were analyzed by qRT-PCR analysis in the rat GI tract divided
into many different sections. The authors identified three categories of
expression patterns in the analysis: ubiquitous, widespread and restricted. The
authors concluded that the adhesion-GPCRs might play an important role in the
function of GI tract and considered them as potential drug targets.
Extended from previous studies on the expression patterns of adhesion-GPCRs
by the same group of authors, this manuscript is an attempt to look at the
detailed expression profiles of adhesion-GPCRs in the GI tract. Thus, the GI tract
was divided into many different segments. The data suggested that
adhesion-GPCR members are expressed ubiquitously, widely, or restricted in the
GI tract. The expression patterns of individual adhesion-GPCR hence implicate
the potential importance in the GI tract function.
The data presented in the manuscript are overall well-controlled and provided a
satisfactory explanation. The information might be useful for the later
physiological and pathological study of GI tract. However, caution should be
taken as the expression data are purely at the transcript levels. There are a few
minor points that need to be clarified better.
Minor Essential Revisions:
1. The primers used in the study are located in the 7TM region. However, it is
well known that many adhesion-GPCR genes undergo extensive alternative
splicing and generate multiple transcripts that include soluble isoforms. Hence,
the primers used might not cover the entire transcript populations. This should be
somehow discussed in the paper. Also, it might be a good idea to list the
expected size of the PCR product in the Table.
2. It will be beneficial to summarize the data in a Table listing the relative
expression levels of all 30 adhesion-GPCR members based on the phylogenetic
groups.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
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