Title: Genetic evidence causally linking gastroesophageal reflux disease to cholecystitis#A two-
sample Mendelian randomization study
Version: 0 Date: 18 Feb 2024
Reviewer's report:
The  paper is quite well written.  The article covers a very interesting and current topic.
Nevertheless, in my opinion, some parts need to be improved, I have some comments:
1) Conclusion: Our study demonstrated a positive correlation between GERD and the likelihood
of developing cholecystitis. These findings may serve as a fundamental basis for comprehending
the intricate interplay between GERD and cholecystitis. However, additional investigations are
warranted to elucidate the precise underlying mechanisms of this plausible causal relationship.
Abstract might be beneficial to include a sentence  that briefly summarizes the key findings of
the study. This can provide readers with a quick overview of the research.
2) In this study, we conducted a two-sample MR study utilizing single SNPs obtained from
genome-wide association studies linked to GERD as IVs, in order to examine the causal impact
of GERD on cholecystitis risk, marking the first such attempt. Please, improve the description
of study aim and underline the novelty of the article.
3) 3. Results
3.1 Detail Information of the Included Instrumental variables (IVs)
This study incorporated a total of 77 independent SNPs from GWASs on GERD as IVs. All
these SNPs were found to be significantly associated with GERD at the genome-wide level
(p<5×10#8) and possessed F-statistics greater than 10, indicating that none of the SNPs were
weak instrumental variables. Supplementary Table S1  ... Please, improve  and clarify this part,
in particular underline the inclusion and the exclusion criteria.
4) 4. Discussion
A two-sample MR analysis was conducted to evaluate the causal association between GERD
and cholecystitis in this study. Our results indicated that genetically predicted GERD was
significantly associated with a higher risk of cholecystitis. The discussion section needs to be
improved.  It could be interesting to record the aim of the study. It is necessary to be more
concise in the presentation of the facts, clarifying the results obtained and comparing them with
previous or similar studies. However, it is interesting to answer the questions that arise from
these results, backed up by published literature.
Title: Genetic evidence causally linking gastroesophageal reflux disease to cholecystitis#A two-
sample Mendelian randomization study
Version: 0 Date: 18 Feb 2024
Reviewer's report:
The  paper is quite well written.  The article covers a very interesting and current topic.
Nevertheless, in my opinion, some parts need to be improved, I have some comments:
1) Conclusion: Our study demonstrated a positive correlation between GERD and the likelihood
of developing cholecystitis. These findings may serve as a fundamental basis for comprehending
the intricate interplay between GERD and cholecystitis. However, additional investigations are
warranted to elucidate the precise underlying mechanisms of this plausible causal relationship.
Abstract might be beneficial to include a sentence  that briefly summarizes the key findings of
the study. This can provide readers with a quick overview of the research.
2) In this study, we conducted a two-sample MR study utilizing single SNPs obtained from
genome-wide association studies linked to GERD as IVs, in order to examine the causal impact
of GERD on cholecystitis risk, marking the first such attempt. Please, improve the description
of study aim and underline the novelty of the article.
3) 3. Results
3.1 Detail Information of the Included Instrumental variables (IVs)
This study incorporated a total of 77 independent SNPs from GWASs on GERD as IVs. All
these SNPs were found to be significantly associated with GERD at the genome-wide level
(p<5×10#8) and possessed F-statistics greater than 10, indicating that none of the SNPs were
weak instrumental variables. Supplementary Table S1  ... Please, improve  and clarify this part,
in particular underline the inclusion and the exclusion criteria.
4) 4. Discussion
A two-sample MR analysis was conducted to evaluate the causal association between GERD
and cholecystitis in this study. Our results indicated that genetically predicted GERD was
significantly associated with a higher risk of cholecystitis. The discussion section needs to be
improved.  It could be interesting to record the aim of the study. It is necessary to be more
concise in the presentation of the facts, clarifying the results obtained and comparing them with
previous or similar studies. However, it is interesting to answer the questions that arise from
these results, backed up by published literature.
Please review this paper.
user
Title: Genetic evidence causally linking gastroesophageal reflux disease to cholecystitis#A two-
sample Mendelian randomization study
Version: 0 Date: 18 Feb 2024
Reviewer's report:
The  paper is quite well written.  The article covers a very interesting and current topic.
Nevertheless, in my opinion, some parts need to be improved, I have some comments:
1) Conclusion: Our study demonstrated a positive correlation between GERD and the likelihood
of developing cholecystitis. These findings may serve as a fundamental basis for comprehending
the intricate interplay between GERD and cholecystitis. However, additional investigations are
warranted to elucidate the precise underlying mechanisms of this plausible causal relationship.
Abstract might be beneficial to include a sentence  that briefly summarizes the key findings of
the study. This can provide readers with a quick overview of the research.
2) In this study, we conducted a two-sample MR study utilizing single SNPs obtained from
genome-wide association studies linked to GERD as IVs, in order to examine the causal impact
of GERD on cholecystitis risk, marking the first such attempt. Please, improve the description
of study aim and underline the novelty of the article.
3) 3. Results
3.1 Detail Information of the Included Instrumental variables (IVs)
This study incorporated a total of 77 independent SNPs from GWASs on GERD as IVs. All
these SNPs were found to be significantly associated with GERD at the genome-wide level
(p<5×10#8) and possessed F-statistics greater than 10, indicating that none of the SNPs were
weak instrumental variables. Supplementary Table S1  ... Please, improve  and clarify this part,
in particular underline the inclusion and the exclusion criteria.
4) 4. Discussion
A two-sample MR analysis was conducted to evaluate the causal association between GERD
and cholecystitis in this study. Our results indicated that genetically predicted GERD was
significantly associated with a higher risk of cholecystitis. The discussion section needs to be
improved.  It could be interesting to record the aim of the study. It is necessary to be more
concise in the presentation of the facts, clarifying the results obtained and comparing them with
previous or similar studies. However, it is interesting to answer the questions that arise from
these results, backed up by published literature.
assistant