Title: Integration of single-cell and bulk RNA sequencing to establish a prognostic signature
based on tumor-associated macrophages in colorectal cancer
Version: 2 Date: 26 Sep 2023
Reviewer's report:
The authors report that TAM-related biomarkers useful for predicting the prognosis of CRC.
I recommend to address following points for the article more informative.
#Please let me know whether the cluster classification is based on previous reports or your own
research.
#We would like to determine if there is a significant difference in the percentage of M2
macrophages between normal and tumor tissues. It appears that there is a significant difference
in M1 macrophages.
#Please explain for the selection of seven gene symbols as factors for calculating the risk score.
#We would like to inquire about your assessment regarding the usefulness of the risk score in
predicting short-term or long-term prognosis, as determined by ROC curve analysis.
#Finally, in the paper, statistical analysis was conducted on three or more groups, and a
significant difference was observed. However, please verify the accuracy of the analysis.
Title: Integration of single-cell and bulk RNA sequencing to establish a prognostic signature
based on tumor-associated macrophages in colorectal cancer
Version: 2 Date: 26 Sep 2023
Reviewer's report:
The authors report that TAM-related biomarkers useful for predicting the prognosis of CRC.
I recommend to address following points for the article more informative.
#Please let me know whether the cluster classification is based on previous reports or your own
research.
#We would like to determine if there is a significant difference in the percentage of M2
macrophages between normal and tumor tissues. It appears that there is a significant difference
in M1 macrophages.
#Please explain for the selection of seven gene symbols as factors for calculating the risk score.
#We would like to inquire about your assessment regarding the usefulness of the risk score in
predicting short-term or long-term prognosis, as determined by ROC curve analysis.
#Finally, in the paper, statistical analysis was conducted on three or more groups, and a
significant difference was observed. However, please verify the accuracy of the analysis.
Please review this paper.
user
Title: Integration of single-cell and bulk RNA sequencing to establish a prognostic signature
based on tumor-associated macrophages in colorectal cancer
Version: 2 Date: 26 Sep 2023
Reviewer's report:
The authors report that TAM-related biomarkers useful for predicting the prognosis of CRC.
I recommend to address following points for the article more informative.
#Please let me know whether the cluster classification is based on previous reports or your own
research.
#We would like to determine if there is a significant difference in the percentage of M2
macrophages between normal and tumor tissues. It appears that there is a significant difference
in M1 macrophages.
#Please explain for the selection of seven gene symbols as factors for calculating the risk score.
#We would like to inquire about your assessment regarding the usefulness of the risk score in
predicting short-term or long-term prognosis, as determined by ROC curve analysis.
#Finally, in the paper, statistical analysis was conducted on three or more groups, and a
significant difference was observed. However, please verify the accuracy of the analysis.
assistant