Title: CircRNA circ-ATAD1 suppresses miR-618 maturation to participate in colorectal cancer
Version: 1 Date: 19 Mar 2021
Reviewer's report:
1.What is RT-qPCR amplification procedures and reaction systems in the section of Materials
and Methods.
2. I don't find the primer sequence. Preferably provided in the materials method.
3. Was BLAST performed or was any reference used ?(If so, please cite)This is important.
4. The materials and methods section should describe how the expression vector is constructed.
Sequencing results can be attached to supplementary materials.
5. Since mature miR-168 plays a major role, why does RNA pull down only detect premature
miR-168?
6. There is clinical data, why didn't  compare circ-ATAD1 with other clinical characteristics to
see if it was associated with other clinical traits besides proliferation?
7. There is an overexpression vector, a plate clone can be made to verify with CCK8.
Title: CircRNA circ-ATAD1 suppresses miR-618 maturation to participate in colorectal cancer
Version: 1 Date: 19 Mar 2021
Reviewer's report:
1.What is RT-qPCR amplification procedures and reaction systems in the section of Materials
and Methods.
2. I don't find the primer sequence. Preferably provided in the materials method.
3. Was BLAST performed or was any reference used ?(If so, please cite)This is important.
4. The materials and methods section should describe how the expression vector is constructed.
Sequencing results can be attached to supplementary materials.
5. Since mature miR-168 plays a major role, why does RNA pull down only detect premature
miR-168?
6. There is clinical data, why didn't  compare circ-ATAD1 with other clinical characteristics to
see if it was associated with other clinical traits besides proliferation?
7. There is an overexpression vector, a plate clone can be made to verify with CCK8.
Please review this paper.
user
Title: CircRNA circ-ATAD1 suppresses miR-618 maturation to participate in colorectal cancer
Version: 1 Date: 19 Mar 2021
Reviewer's report:
1.What is RT-qPCR amplification procedures and reaction systems in the section of Materials
and Methods.
2. I don't find the primer sequence. Preferably provided in the materials method.
3. Was BLAST performed or was any reference used ?(If so, please cite)This is important.
4. The materials and methods section should describe how the expression vector is constructed.
Sequencing results can be attached to supplementary materials.
5. Since mature miR-168 plays a major role, why does RNA pull down only detect premature
miR-168?
6. There is clinical data, why didn't  compare circ-ATAD1 with other clinical characteristics to
see if it was associated with other clinical traits besides proliferation?
7. There is an overexpression vector, a plate clone can be made to verify with CCK8.
assistant