Reviewer's report 
 Grey scale enhancement by a new self-made contrast agent in early cirrhotic stage of rabbit liver Title: 
 2  24 January 2007 Version: Date: 
 Adrian Lim Reviewer: 
Reviewer's report: 
The numbers are small and statistical review by a statistician to ensure that the appropriate statistical 
analysis has been performed is suggested. I note that there are 5 groups (F0 to F4) and only 12 rabbits 
used. Please provide the number of subjects in each category in table 1. 
The data would be enhanced if table 1 could be presented graphically (also you only need to include the 
statistics which are signifcant). The discussion although improved needs to include some of the more 
recent papers which investigate hepatic transit times in liver fibrosis within humans. More information on the 
make and content of the microbubble would also enhance the introduction and discussion. A limitations 
section should also be included. 
 Unable to decide on acceptance or rejection until the authors have responded to the major What next?: 
compulsory revisions 
 An article of limited interest Level of interest: 
 Not suitable for publication unless extensively edited Quality of written English: 
 Yes, but I do not feel adequately qualified to assess the statistics. Statistical review: 
Declaration of competing interests: 
No 
Reviewer's report 
 Grey scale enhancement by a new self-made contrast agent in early cirrhotic stage of rabbit liver Title: 
 2  24 January 2007 Version: Date: 
 Adrian Lim Reviewer: 
Reviewer's report: 
The numbers are small and statistical review by a statistician to ensure that the appropriate statistical 
analysis has been performed is suggested. I note that there are 5 groups (F0 to F4) and only 12 rabbits 
used. Please provide the number of subjects in each category in table 1. 
The data would be enhanced if table 1 could be presented graphically (also you only need to include the 
statistics which are signifcant). The discussion although improved needs to include some of the more 
recent papers which investigate hepatic transit times in liver fibrosis within humans. More information on the 
make and content of the microbubble would also enhance the introduction and discussion. A limitations 
section should also be included. 
 Unable to decide on acceptance or rejection until the authors have responded to the major What next?: 
compulsory revisions 
 An article of limited interest Level of interest: 
 Not suitable for publication unless extensively edited Quality of written English: 
 Yes, but I do not feel adequately qualified to assess the statistics. Statistical review: 
Declaration of competing interests: 
No 
Please review this paper.
user
Reviewer's report 
 Grey scale enhancement by a new self-made contrast agent in early cirrhotic stage of rabbit liver Title: 
 2  24 January 2007 Version: Date: 
 Adrian Lim Reviewer: 
Reviewer's report: 
The numbers are small and statistical review by a statistician to ensure that the appropriate statistical 
analysis has been performed is suggested. I note that there are 5 groups (F0 to F4) and only 12 rabbits 
used. Please provide the number of subjects in each category in table 1. 
The data would be enhanced if table 1 could be presented graphically (also you only need to include the 
statistics which are signifcant). The discussion although improved needs to include some of the more 
recent papers which investigate hepatic transit times in liver fibrosis within humans. More information on the 
make and content of the microbubble would also enhance the introduction and discussion. A limitations 
section should also be included. 
 Unable to decide on acceptance or rejection until the authors have responded to the major What next?: 
compulsory revisions 
 An article of limited interest Level of interest: 
 Not suitable for publication unless extensively edited Quality of written English: 
 Yes, but I do not feel adequately qualified to assess the statistics. Statistical review: 
Declaration of competing interests: 
No 
assistant