Title: Validity of rapid urease test using swab of gastric mucus to mucosal forceps and 13C-
urease breath test: a multicenter prospective observational study
Version: 2 Date: 27 Jun 2024
Reviewer's report:
1. Correlation with UBT and biopsy-RUT: The paper suggests that Swab-RUT shows good
correlation with both UBT and biopsy-RUT. This indicates that Swab-RUT could be a reliable
alternative to these tests when biopsy samples are not available or not preferred.
2. Limitation due to biopsy-RUT: Since a significant portion of subjects (>50%) did not undergo
biopsy-RUT, the study lacks direct biopsy confirmation for these individuals. Biopsy-RUT or
histology is typically considered the gold standard for detecting H. pylori infection because it
directly tests the gastric tissue where the bacteria reside.
3. Impact on accuracy assessment: Without biopsy-RUT results for a large portion of the
subjects, it's challenging to accurately determine the sensitivity, specificity, and overall
diagnostic accuracy of Swab-RUT for detecting H. pylori infection. The accuracy could
potentially be overestimated if biopsy-RUT-negative cases were missed among those who did
not undergo biopsy.
Conclusion: The findings suggest that while Swab-RUT correlates well with other tests like
UBT and biopsy-RUT, its true accuracy in diagnosing H. pylori infection remains uncertain
without biopsy confirmation. This uncertainty could affect treatment decisions, as biopsy results
are crucial for guiding appropriate therapy.
Title: Validity of rapid urease test using swab of gastric mucus to mucosal forceps and 13C-
urease breath test: a multicenter prospective observational study
Version: 2 Date: 27 Jun 2024
Reviewer's report:
1. Correlation with UBT and biopsy-RUT: The paper suggests that Swab-RUT shows good
correlation with both UBT and biopsy-RUT. This indicates that Swab-RUT could be a reliable
alternative to these tests when biopsy samples are not available or not preferred.
2. Limitation due to biopsy-RUT: Since a significant portion of subjects (>50%) did not undergo
biopsy-RUT, the study lacks direct biopsy confirmation for these individuals. Biopsy-RUT or
histology is typically considered the gold standard for detecting H. pylori infection because it
directly tests the gastric tissue where the bacteria reside.
3. Impact on accuracy assessment: Without biopsy-RUT results for a large portion of the
subjects, it's challenging to accurately determine the sensitivity, specificity, and overall
diagnostic accuracy of Swab-RUT for detecting H. pylori infection. The accuracy could
potentially be overestimated if biopsy-RUT-negative cases were missed among those who did
not undergo biopsy.
Conclusion: The findings suggest that while Swab-RUT correlates well with other tests like
UBT and biopsy-RUT, its true accuracy in diagnosing H. pylori infection remains uncertain
without biopsy confirmation. This uncertainty could affect treatment decisions, as biopsy results
are crucial for guiding appropriate therapy.
Please review this paper.
user
Title: Validity of rapid urease test using swab of gastric mucus to mucosal forceps and 13C-
urease breath test: a multicenter prospective observational study
Version: 2 Date: 27 Jun 2024
Reviewer's report:
1. Correlation with UBT and biopsy-RUT: The paper suggests that Swab-RUT shows good
correlation with both UBT and biopsy-RUT. This indicates that Swab-RUT could be a reliable
alternative to these tests when biopsy samples are not available or not preferred.
2. Limitation due to biopsy-RUT: Since a significant portion of subjects (>50%) did not undergo
biopsy-RUT, the study lacks direct biopsy confirmation for these individuals. Biopsy-RUT or
histology is typically considered the gold standard for detecting H. pylori infection because it
directly tests the gastric tissue where the bacteria reside.
3. Impact on accuracy assessment: Without biopsy-RUT results for a large portion of the
subjects, it's challenging to accurately determine the sensitivity, specificity, and overall
diagnostic accuracy of Swab-RUT for detecting H. pylori infection. The accuracy could
potentially be overestimated if biopsy-RUT-negative cases were missed among those who did
not undergo biopsy.
Conclusion: The findings suggest that while Swab-RUT correlates well with other tests like
UBT and biopsy-RUT, its true accuracy in diagnosing H. pylori infection remains uncertain
without biopsy confirmation. This uncertainty could affect treatment decisions, as biopsy results
are crucial for guiding appropriate therapy.
assistant