Title: Sinomenine hydrochloride improves DSS-induced colitis in mice through inhibition of
the Notch signaling pathway
Version: 3 Date: 02 Nov 2024
Reviewer:  Panagiotis Tsibouris
Reviewer's report:
Title Rodents donot suffer from Uc but can developed colitis mimicing UC Thus UC model
should be better used Abstract Accodingly DSS colitis should be used and not UC! Abstract
Authors should provide numerical and p values for statistically significant relations Methods
Materials should be better presented on their first appearance in the experiment It is not clear in
which way intragastric infusion was performed Was a scale to record overt bleeding used? Was
number of imes rodents open their bowels recorded? DAI score should be better descibed as well
as CMDI Histology should be described in detail Also PCRs should be described in more detail
if possible Statistics Six subjests is a too small number to draw any conclusions Have authors
calculated power of the study or their survey is a preliminary one?  Results Authors should
provide numerical and p values for statistically significant relations in the text Any difference
between the two doses of SH should be reported Immunohistochemistry pictures could be also
included
Title: Sinomenine hydrochloride improves DSS-induced colitis in mice through inhibition of
the Notch signaling pathway
Version: 3 Date: 02 Nov 2024
Reviewer:  Panagiotis Tsibouris
Reviewer's report:
Title Rodents donot suffer from Uc but can developed colitis mimicing UC Thus UC model
should be better used Abstract Accodingly DSS colitis should be used and not UC! Abstract
Authors should provide numerical and p values for statistically significant relations Methods
Materials should be better presented on their first appearance in the experiment It is not clear in
which way intragastric infusion was performed Was a scale to record overt bleeding used? Was
number of imes rodents open their bowels recorded? DAI score should be better descibed as well
as CMDI Histology should be described in detail Also PCRs should be described in more detail
if possible Statistics Six subjests is a too small number to draw any conclusions Have authors
calculated power of the study or their survey is a preliminary one?  Results Authors should
provide numerical and p values for statistically significant relations in the text Any difference
between the two doses of SH should be reported Immunohistochemistry pictures could be also
included
Please review this paper.
user
Title: Sinomenine hydrochloride improves DSS-induced colitis in mice through inhibition of
the Notch signaling pathway
Version: 3 Date: 02 Nov 2024
Reviewer:  Panagiotis Tsibouris
Reviewer's report:
Title Rodents donot suffer from Uc but can developed colitis mimicing UC Thus UC model
should be better used Abstract Accodingly DSS colitis should be used and not UC! Abstract
Authors should provide numerical and p values for statistically significant relations Methods
Materials should be better presented on their first appearance in the experiment It is not clear in
which way intragastric infusion was performed Was a scale to record overt bleeding used? Was
number of imes rodents open their bowels recorded? DAI score should be better descibed as well
as CMDI Histology should be described in detail Also PCRs should be described in more detail
if possible Statistics Six subjests is a too small number to draw any conclusions Have authors
calculated power of the study or their survey is a preliminary one?  Results Authors should
provide numerical and p values for statistically significant relations in the text Any difference
between the two doses of SH should be reported Immunohistochemistry pictures could be also
included
assistant