Title: Expression profile and N6-methyadenosine modification of Circular RNA analysis in
MAFLD
Version: 0 Date: 07 Dec 2024
Reviewer's report:
This manuscript explores the expression profile of circular RNAs (circRNAs) and their N6-
methyladenosine (m6A) modifications in metabolic-associated fatty liver disease (MAFLD).
The study combines bioinformatics analysis with experimental validation, offering fresh insights
into the molecular mechanisms of MAFLD. The manuscript is well-structured, with credible
conclusions and potential implications for clinical diagnosis and treatment. However, several
aspects need improvement to enhance the overall quality of the paper.
1. Some figures (e.g., Figure 2A’s density plots and Figure 3’s GO/KEGG analysis) are overly
dense and difficult to interpret. Please improve the legends and annotations to make the data
easier to understand.
In Figure 4, the statistical graphs for RT-qPCR and MeRIP-qPCR lack clear indications of
significance. Consider adding specific p-values for better clarity.
2. The description of the microarray analysis and SRAMP prediction tool lacks specific details.
Please provide the parameters and criteria used for these analyses.
The workflow and data analysis for the MeRIP-qPCR experiments are too brief. A more detailed
explanation is needed.
3. The study does not adequately discuss its limitations, such as the relatively small sample size
(only five cases per group), which might affect the generalizability of the results. Additionally,
the causal relationship between m6A modifications and circRNA expression remains unclear.
Addressing these points in the discussion section would improve the manuscript.
This study provides valuable new insights and has scientific merit, but improvements in data
presentation, methodological details, and discussion depth are necessary.  I recommend minor
revisions before it can be considered for publication.
Title: Expression profile and N6-methyadenosine modification of Circular RNA analysis in
MAFLD
Version: 0 Date: 07 Dec 2024
Reviewer's report:
This manuscript explores the expression profile of circular RNAs (circRNAs) and their N6-
methyladenosine (m6A) modifications in metabolic-associated fatty liver disease (MAFLD).
The study combines bioinformatics analysis with experimental validation, offering fresh insights
into the molecular mechanisms of MAFLD. The manuscript is well-structured, with credible
conclusions and potential implications for clinical diagnosis and treatment. However, several
aspects need improvement to enhance the overall quality of the paper.
1. Some figures (e.g., Figure 2A’s density plots and Figure 3’s GO/KEGG analysis) are overly
dense and difficult to interpret. Please improve the legends and annotations to make the data
easier to understand.
In Figure 4, the statistical graphs for RT-qPCR and MeRIP-qPCR lack clear indications of
significance. Consider adding specific p-values for better clarity.
2. The description of the microarray analysis and SRAMP prediction tool lacks specific details.
Please provide the parameters and criteria used for these analyses.
The workflow and data analysis for the MeRIP-qPCR experiments are too brief. A more detailed
explanation is needed.
3. The study does not adequately discuss its limitations, such as the relatively small sample size
(only five cases per group), which might affect the generalizability of the results. Additionally,
the causal relationship between m6A modifications and circRNA expression remains unclear.
Addressing these points in the discussion section would improve the manuscript.
This study provides valuable new insights and has scientific merit, but improvements in data
presentation, methodological details, and discussion depth are necessary.  I recommend minor
revisions before it can be considered for publication.
Please review this paper.
user
Title: Expression profile and N6-methyadenosine modification of Circular RNA analysis in
MAFLD
Version: 0 Date: 07 Dec 2024
Reviewer's report:
This manuscript explores the expression profile of circular RNAs (circRNAs) and their N6-
methyladenosine (m6A) modifications in metabolic-associated fatty liver disease (MAFLD).
The study combines bioinformatics analysis with experimental validation, offering fresh insights
into the molecular mechanisms of MAFLD. The manuscript is well-structured, with credible
conclusions and potential implications for clinical diagnosis and treatment. However, several
aspects need improvement to enhance the overall quality of the paper.
1. Some figures (e.g., Figure 2A’s density plots and Figure 3’s GO/KEGG analysis) are overly
dense and difficult to interpret. Please improve the legends and annotations to make the data
easier to understand.
In Figure 4, the statistical graphs for RT-qPCR and MeRIP-qPCR lack clear indications of
significance. Consider adding specific p-values for better clarity.
2. The description of the microarray analysis and SRAMP prediction tool lacks specific details.
Please provide the parameters and criteria used for these analyses.
The workflow and data analysis for the MeRIP-qPCR experiments are too brief. A more detailed
explanation is needed.
3. The study does not adequately discuss its limitations, such as the relatively small sample size
(only five cases per group), which might affect the generalizability of the results. Additionally,
the causal relationship between m6A modifications and circRNA expression remains unclear.
Addressing these points in the discussion section would improve the manuscript.
This study provides valuable new insights and has scientific merit, but improvements in data
presentation, methodological details, and discussion depth are necessary.  I recommend minor
revisions before it can be considered for publication.
assistant