Title: Change of monocytes/macrophages in ulcerative colitis patients with symptoms of anxiety
and depression
Version: 1 Date: 18 Sep 2022
Reviewer's report:
The authors conducted a study focusing on the relationship between patients with ulcerative
colitis and anxiety/depressive symptoms and showed that patients with anxiety/depressive
symptoms tend to have worse QOL and worse clinical symptoms of ulcerative colitis. It
was also shown that concentrations of inflammatory cytokines such as TNF-#, IL-1#, and
IL-6 were significantly higher in UC patients with anxiety/depressive symptoms. Furthermore,
patients with anxiety and depressive symptoms had a higher percentage of CD14++CD16+ and
CD14+CD16++ monocytes, a higher percentage of CD68+ cells in the intestinal mucosal layer,
and a higher M1/M2 ratio.
These are very interesting results, but some additional confirmation is needed at the time of
publication.
1) Could you please tell me if the patients in your study had any history of anxiolytic use or
psychiatric consultation?
2) Since you mentioned that there was a difference in steroid use between the two groups, what
was the dose and duration of steroid use?
Title: Change of monocytes/macrophages in ulcerative colitis patients with symptoms of anxiety
and depression
Version: 1 Date: 18 Sep 2022
Reviewer's report:
The authors conducted a study focusing on the relationship between patients with ulcerative
colitis and anxiety/depressive symptoms and showed that patients with anxiety/depressive
symptoms tend to have worse QOL and worse clinical symptoms of ulcerative colitis. It
was also shown that concentrations of inflammatory cytokines such as TNF-#, IL-1#, and
IL-6 were significantly higher in UC patients with anxiety/depressive symptoms. Furthermore,
patients with anxiety and depressive symptoms had a higher percentage of CD14++CD16+ and
CD14+CD16++ monocytes, a higher percentage of CD68+ cells in the intestinal mucosal layer,
and a higher M1/M2 ratio.
These are very interesting results, but some additional confirmation is needed at the time of
publication.
1) Could you please tell me if the patients in your study had any history of anxiolytic use or
psychiatric consultation?
2) Since you mentioned that there was a difference in steroid use between the two groups, what
was the dose and duration of steroid use?
Please review this paper.
user
Title: Change of monocytes/macrophages in ulcerative colitis patients with symptoms of anxiety
and depression
Version: 1 Date: 18 Sep 2022
Reviewer's report:
The authors conducted a study focusing on the relationship between patients with ulcerative
colitis and anxiety/depressive symptoms and showed that patients with anxiety/depressive
symptoms tend to have worse QOL and worse clinical symptoms of ulcerative colitis. It
was also shown that concentrations of inflammatory cytokines such as TNF-#, IL-1#, and
IL-6 were significantly higher in UC patients with anxiety/depressive symptoms. Furthermore,
patients with anxiety and depressive symptoms had a higher percentage of CD14++CD16+ and
CD14+CD16++ monocytes, a higher percentage of CD68+ cells in the intestinal mucosal layer,
and a higher M1/M2 ratio.
These are very interesting results, but some additional confirmation is needed at the time of
publication.
1) Could you please tell me if the patients in your study had any history of anxiolytic use or
psychiatric consultation?
2) Since you mentioned that there was a difference in steroid use between the two groups, what
was the dose and duration of steroid use?
assistant