Title: Macrophage polarization toward M1 phenotype in T cell transfer colitis model
Version: 1 Date: 29 Oct 2023
Reviewer:  Mousumi Mandal
Reviewer's report:
The present study designed to explore macrophage polarization toward M1 phenotype in T
cell transfer colitis model. Authors have analyzed the inflammatory pattern (colonoscopy and
histology), inflammatory cell phenotype by flowcytometry and the levels of inflammatory
markers. They concluded that M1 macrophage polarization in the colonic lamina propria and
this M1 phenotype play vital role in T cell transfer colitis model. The study was well conducted,
and their findings could support the conclusion. However, several points need to be improved.
Please, see below the comments.
1. Colonoscopic pictures (Fig1. B-D) are needed to keep in first (Fig1. A) from where the scores
have been analyzed. Similarly, histological score plot should be represented after the histological
images in figure1.
2. Gating strategy for the flowcytometric analysis of LP immune cells has been clearly depicted
in Fig3A and the numbers of colonic LP macrophages, inflammatory monocytes neutrophils and
T cells has been analyzed in Fig3B–E. It will be good to add the exact flow cytometric dot plots
of these cells for better visualization. In that case Fig3 might be divided to another figure.
3. It is needed to add the approval no with date of Institutional Animal Care and Use Committee
in the materials and methods part of the manuscript.
Title: Macrophage polarization toward M1 phenotype in T cell transfer colitis model
Version: 1 Date: 29 Oct 2023
Reviewer:  Mousumi Mandal
Reviewer's report:
The present study designed to explore macrophage polarization toward M1 phenotype in T
cell transfer colitis model. Authors have analyzed the inflammatory pattern (colonoscopy and
histology), inflammatory cell phenotype by flowcytometry and the levels of inflammatory
markers. They concluded that M1 macrophage polarization in the colonic lamina propria and
this M1 phenotype play vital role in T cell transfer colitis model. The study was well conducted,
and their findings could support the conclusion. However, several points need to be improved.
Please, see below the comments.
1. Colonoscopic pictures (Fig1. B-D) are needed to keep in first (Fig1. A) from where the scores
have been analyzed. Similarly, histological score plot should be represented after the histological
images in figure1.
2. Gating strategy for the flowcytometric analysis of LP immune cells has been clearly depicted
in Fig3A and the numbers of colonic LP macrophages, inflammatory monocytes neutrophils and
T cells has been analyzed in Fig3B–E. It will be good to add the exact flow cytometric dot plots
of these cells for better visualization. In that case Fig3 might be divided to another figure.
3. It is needed to add the approval no with date of Institutional Animal Care and Use Committee
in the materials and methods part of the manuscript.
Please review this paper.
user
Title: Macrophage polarization toward M1 phenotype in T cell transfer colitis model
Version: 1 Date: 29 Oct 2023
Reviewer:  Mousumi Mandal
Reviewer's report:
The present study designed to explore macrophage polarization toward M1 phenotype in T
cell transfer colitis model. Authors have analyzed the inflammatory pattern (colonoscopy and
histology), inflammatory cell phenotype by flowcytometry and the levels of inflammatory
markers. They concluded that M1 macrophage polarization in the colonic lamina propria and
this M1 phenotype play vital role in T cell transfer colitis model. The study was well conducted,
and their findings could support the conclusion. However, several points need to be improved.
Please, see below the comments.
1. Colonoscopic pictures (Fig1. B-D) are needed to keep in first (Fig1. A) from where the scores
have been analyzed. Similarly, histological score plot should be represented after the histological
images in figure1.
2. Gating strategy for the flowcytometric analysis of LP immune cells has been clearly depicted
in Fig3A and the numbers of colonic LP macrophages, inflammatory monocytes neutrophils and
T cells has been analyzed in Fig3B–E. It will be good to add the exact flow cytometric dot plots
of these cells for better visualization. In that case Fig3 might be divided to another figure.
3. It is needed to add the approval no with date of Institutional Animal Care and Use Committee
in the materials and methods part of the manuscript.
assistant