Reviewer's report
Title:Role of inducible nitric oxide synthase pathway on methotrexate-induced
intestinal mucositis in rodents
Version: 1Date:24 May 2011
Reviewer: Guntram Grassl
Reviewer's report:
In their manuscript “Role of inducible nitric oxide synthase pathway on
methotrexate-
induced intestinal mucositis in rodents” the authors demonstrate that NOS
inhibitors ameliorate methotrexate-induced intestinal damage. In addition they
show that iNOS-/- mice do not develop strong inflammation upon methotrexate
treatment.
The manuscripts provides novel insights into the role of NO in
methotrexate-triggered mucosal inflammation. However, apparently some parts
were mixed up during the preparation of the manuscript. This needs to be
corrected, so that these parts can be evaluated properly.
Major comments:
1. Mix up in Figure legends/missing figures: Figure legend 4 does not correspond
to any figure in the manuscript.
2. Figure legend 5 corresponds to figure 4.
3. Figure 5 does not have a legend at all.
4. Figures 4 and 5 and their respective Results sections are switched.
Minor comments:
1. The references don’t correspond to the numbers in the text in some cases (eg
introduction, page 4, reference 18 corresponds to reference 21 in the Reference
list) – all references should be checked and corrected.
2. Figure 3: legend reads units/mg of tissue, figure reads /g of tissue. This needs
to be corrected.
3. The addition of labelling within the figure in figure 4 would make it easier to
understand the panels.
4. Table 2: stating the range as (…+/-…) would make it clearer
5. It’s not clear if Figure 3 has data from duodenum, jejunum and ileum combined
or only one of the sections.
6. Why are there only data of the jejunum in Table 3?
7. Table 4: commas should be replaced by points.
8. ‘C57BL’ should probably read ‘C57BL/6’
9. Discussion, page 14: ‘tumor necrosis alpha’ should read ‘tumor necrosis factor
alpha’
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Role of inducible nitric oxide synthase pathway on methotrexate-induced
intestinal mucositis in rodents
Version: 1Date:24 May 2011
Reviewer: Guntram Grassl
Reviewer's report:
In their manuscript “Role of inducible nitric oxide synthase pathway on
methotrexate-
induced intestinal mucositis in rodents” the authors demonstrate that NOS
inhibitors ameliorate methotrexate-induced intestinal damage. In addition they
show that iNOS-/- mice do not develop strong inflammation upon methotrexate
treatment.
The manuscripts provides novel insights into the role of NO in
methotrexate-triggered mucosal inflammation. However, apparently some parts
were mixed up during the preparation of the manuscript. This needs to be
corrected, so that these parts can be evaluated properly.
Major comments:
1. Mix up in Figure legends/missing figures: Figure legend 4 does not correspond
to any figure in the manuscript.
2. Figure legend 5 corresponds to figure 4.
3. Figure 5 does not have a legend at all.
4. Figures 4 and 5 and their respective Results sections are switched.
Minor comments:
1. The references don’t correspond to the numbers in the text in some cases (eg
introduction, page 4, reference 18 corresponds to reference 21 in the Reference
list) – all references should be checked and corrected.
2. Figure 3: legend reads units/mg of tissue, figure reads /g of tissue. This needs
to be corrected.
3. The addition of labelling within the figure in figure 4 would make it easier to
understand the panels.
4. Table 2: stating the range as (…+/-…) would make it clearer
5. It’s not clear if Figure 3 has data from duodenum, jejunum and ileum combined
or only one of the sections.
6. Why are there only data of the jejunum in Table 3?
7. Table 4: commas should be replaced by points.
8. ‘C57BL’ should probably read ‘C57BL/6’
9. Discussion, page 14: ‘tumor necrosis alpha’ should read ‘tumor necrosis factor
alpha’
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Role of inducible nitric oxide synthase pathway on methotrexate-induced
intestinal mucositis in rodents
Version: 1Date:24 May 2011
Reviewer: Guntram Grassl
Reviewer's report:
In their manuscript “Role of inducible nitric oxide synthase pathway on
methotrexate-
induced intestinal mucositis in rodents” the authors demonstrate that NOS
inhibitors ameliorate methotrexate-induced intestinal damage. In addition they
show that iNOS-/- mice do not develop strong inflammation upon methotrexate
treatment.
The manuscripts provides novel insights into the role of NO in
methotrexate-triggered mucosal inflammation. However, apparently some parts
were mixed up during the preparation of the manuscript. This needs to be
corrected, so that these parts can be evaluated properly.
Major comments:
1. Mix up in Figure legends/missing figures: Figure legend 4 does not correspond
to any figure in the manuscript.
2. Figure legend 5 corresponds to figure 4.
3. Figure 5 does not have a legend at all.
4. Figures 4 and 5 and their respective Results sections are switched.
Minor comments:
1. The references don’t correspond to the numbers in the text in some cases (eg
introduction, page 4, reference 18 corresponds to reference 21 in the Reference
list) – all references should be checked and corrected.
2. Figure 3: legend reads units/mg of tissue, figure reads /g of tissue. This needs
to be corrected.
3. The addition of labelling within the figure in figure 4 would make it easier to
understand the panels.
4. Table 2: stating the range as (…+/-…) would make it clearer
5. It’s not clear if Figure 3 has data from duodenum, jejunum and ileum combined
or only one of the sections.
6. Why are there only data of the jejunum in Table 3?
7. Table 4: commas should be replaced by points.
8. ‘C57BL’ should probably read ‘C57BL/6’
9. Discussion, page 14: ‘tumor necrosis alpha’ should read ‘tumor necrosis factor
alpha’
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
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