Title: Optimization of the Cholesterol Gallstone Model in C57BL/6 Mice and Evaluation of
Lactobacillus Intervention Effects
Version: 2 Date: 15 Jan 2025
Reviewer's report:
Comments to the Authors
I recommend its potential publication. Some comments are as follows.
1. In the second phase of experiment, how many C57BL/6 mice were included?
2. In the whole experimental stage, how many C57BL/6 mice had cholesterol gallstone
formation?
3. In the section “Liver Pathology in Mice”, the authors should explain which group corresponds
to the Figure 6E.
4. In this study, the authors used only one strain of Lactobacillus to evaluate the effects on
cholesterol gallstone formation. The word “systematically” is misfit.
5. In this study, the authors used only one strain of Lactobacillus to evaluate the effects on
cholesterol gallstone formation. “Probiotic” should be revised as “Lactobacillus” in the text and
title.
6. The word “First” appears twice in the discussion section, and the authors should change the
“First” to “Second” in the second paragraph.
7. The words “with the goal of …” are meaningless in the introduction section. Delete them.
Title: Optimization of the Cholesterol Gallstone Model in C57BL/6 Mice and Evaluation of
Lactobacillus Intervention Effects
Version: 2 Date: 15 Jan 2025
Reviewer's report:
Comments to the Authors
I recommend its potential publication. Some comments are as follows.
1. In the second phase of experiment, how many C57BL/6 mice were included?
2. In the whole experimental stage, how many C57BL/6 mice had cholesterol gallstone
formation?
3. In the section “Liver Pathology in Mice”, the authors should explain which group corresponds
to the Figure 6E.
4. In this study, the authors used only one strain of Lactobacillus to evaluate the effects on
cholesterol gallstone formation. The word “systematically” is misfit.
5. In this study, the authors used only one strain of Lactobacillus to evaluate the effects on
cholesterol gallstone formation. “Probiotic” should be revised as “Lactobacillus” in the text and
title.
6. The word “First” appears twice in the discussion section, and the authors should change the
“First” to “Second” in the second paragraph.
7. The words “with the goal of …” are meaningless in the introduction section. Delete them.
Please review this paper.
user
Title: Optimization of the Cholesterol Gallstone Model in C57BL/6 Mice and Evaluation of
Lactobacillus Intervention Effects
Version: 2 Date: 15 Jan 2025
Reviewer's report:
Comments to the Authors
I recommend its potential publication. Some comments are as follows.
1. In the second phase of experiment, how many C57BL/6 mice were included?
2. In the whole experimental stage, how many C57BL/6 mice had cholesterol gallstone
formation?
3. In the section “Liver Pathology in Mice”, the authors should explain which group corresponds
to the Figure 6E.
4. In this study, the authors used only one strain of Lactobacillus to evaluate the effects on
cholesterol gallstone formation. The word “systematically” is misfit.
5. In this study, the authors used only one strain of Lactobacillus to evaluate the effects on
cholesterol gallstone formation. “Probiotic” should be revised as “Lactobacillus” in the text and
title.
6. The word “First” appears twice in the discussion section, and the authors should change the
“First” to “Second” in the second paragraph.
7. The words “with the goal of …” are meaningless in the introduction section. Delete them.
assistant