Title: Nuclear respiratory factor 1 regulates super enhancer-controlled SPIDR to protect
hepatocellular carcinoma cells from oxidative stress
Version: 0 Date: 24 Oct 2023
Reviewer's report:
This manuscript is of very interest for this topic, but major points should be addressed:
1, Some sentences, such as “SEs are characterized by high levels of acetylation of lysine 27 on
histone 3 (H3K27ac)” described by the authors do not sound to be scientific, because SE is a
segment of DNAs, it cannot be subjected to acetylation of lysine 27 on histone 3 (H3K27a).
Please use the paper sentences to describe the discovery within the real scientific language.
2. In Figure 6, the quality control of ChIP-qPCR should be provided along with a visualized
image.
3. All the relevant protein abundances should be examined by Western blotting and also
presented along with their results obtained from mRNA expression levels (e.g., Figures 3, 5,
6 and 7).
Title: Nuclear respiratory factor 1 regulates super enhancer-controlled SPIDR to protect
hepatocellular carcinoma cells from oxidative stress
Version: 0 Date: 24 Oct 2023
Reviewer's report:
This manuscript is of very interest for this topic, but major points should be addressed:
1, Some sentences, such as “SEs are characterized by high levels of acetylation of lysine 27 on
histone 3 (H3K27ac)” described by the authors do not sound to be scientific, because SE is a
segment of DNAs, it cannot be subjected to acetylation of lysine 27 on histone 3 (H3K27a).
Please use the paper sentences to describe the discovery within the real scientific language.
2. In Figure 6, the quality control of ChIP-qPCR should be provided along with a visualized
image.
3. All the relevant protein abundances should be examined by Western blotting and also
presented along with their results obtained from mRNA expression levels (e.g., Figures 3, 5,
6 and 7).
Please review this paper.
user
Title: Nuclear respiratory factor 1 regulates super enhancer-controlled SPIDR to protect
hepatocellular carcinoma cells from oxidative stress
Version: 0 Date: 24 Oct 2023
Reviewer's report:
This manuscript is of very interest for this topic, but major points should be addressed:
1, Some sentences, such as “SEs are characterized by high levels of acetylation of lysine 27 on
histone 3 (H3K27ac)” described by the authors do not sound to be scientific, because SE is a
segment of DNAs, it cannot be subjected to acetylation of lysine 27 on histone 3 (H3K27a).
Please use the paper sentences to describe the discovery within the real scientific language.
2. In Figure 6, the quality control of ChIP-qPCR should be provided along with a visualized
image.
3. All the relevant protein abundances should be examined by Western blotting and also
presented along with their results obtained from mRNA expression levels (e.g., Figures 3, 5,
6 and 7).
assistant