Reviewer's report
 The plasma membrane carbonic anhydrase in murine hepatocytes identified as isozyme XIV Title:
Authors:
Seppo Parkkila (seppo.parkkila@uta.fi )
Antti J Kivela (akivela@paju.oulu.fi )
Kari Kaunisto (kari.kaunisto@oulu.fi )
Anna-Kaisa Parkkila (akparkkila@hotmail.com )
Jukka Hakkola (jukka.hakkola@oulu.fi )
Hannu Rajaniemi (hannu.rajaniemi@oulu.fi )
Abdul Waheed (waheeda@slu.edu )
William S Sly (slyws@slu.edu )
 1  8 May 2002 Version: Date:
 Claudiu Supuran Reviewer:
 not specified Level of interest:
 Accept after discretionary revisions Advice on publication:
Recommendation: PUBLISH with minor revision 
Comments: This is an interesting work regarding the identification of the isozyme type responsible for 
CA activity in the hepatocyte plasma membranes. Up to now it was considered that this is due to 
iszoyme CA IV, but the present authors clearly show that this is due to the recently isoalted isozyme CA 
XIV. The experimental techniques described are straightforward and properly described. 
Furtermore: 
1. Are the conclusions drawn adequately supported by the data shown: 
Yes, they are. 
2. Are sufficcient details provided to allow replication of the work or comparison with related analyses: 
Yes. 
3. Does the manuscript adhere to the relevant standards for reporting and data deposition: 
yes. 
4. Is the writing acceptable? 
No, references provided are insufficient. For example, in several recent reviews, the cellular distribution 
and physiological functions of the various CA isozymes have been extensively discussed. These works 
must be cited, such as for example: C.T. Supuran, A. Scozzafava, Exp. Opin. Ther. Patents 2002, 12, 217-242. 
C.T. Supuran, A. Scozzafava, Exp. Opin. Ther. Patents 2000, 10, 575-600. 
Competing interests:
None declared.
Reviewer's report
 The plasma membrane carbonic anhydrase in murine hepatocytes identified as isozyme XIV Title:
Authors:
Seppo Parkkila (seppo.parkkila@uta.fi )
Antti J Kivela (akivela@paju.oulu.fi )
Kari Kaunisto (kari.kaunisto@oulu.fi )
Anna-Kaisa Parkkila (akparkkila@hotmail.com )
Jukka Hakkola (jukka.hakkola@oulu.fi )
Hannu Rajaniemi (hannu.rajaniemi@oulu.fi )
Abdul Waheed (waheeda@slu.edu )
William S Sly (slyws@slu.edu )
 1  8 May 2002 Version: Date:
 Claudiu Supuran Reviewer:
 not specified Level of interest:
 Accept after discretionary revisions Advice on publication:
Recommendation: PUBLISH with minor revision 
Comments: This is an interesting work regarding the identification of the isozyme type responsible for 
CA activity in the hepatocyte plasma membranes. Up to now it was considered that this is due to 
iszoyme CA IV, but the present authors clearly show that this is due to the recently isoalted isozyme CA 
XIV. The experimental techniques described are straightforward and properly described. 
Furtermore: 
1. Are the conclusions drawn adequately supported by the data shown: 
Yes, they are. 
2. Are sufficcient details provided to allow replication of the work or comparison with related analyses: 
Yes. 
3. Does the manuscript adhere to the relevant standards for reporting and data deposition: 
yes. 
4. Is the writing acceptable? 
No, references provided are insufficient. For example, in several recent reviews, the cellular distribution 
and physiological functions of the various CA isozymes have been extensively discussed. These works 
must be cited, such as for example: C.T. Supuran, A. Scozzafava, Exp. Opin. Ther. Patents 2002, 12, 217-242. 
C.T. Supuran, A. Scozzafava, Exp. Opin. Ther. Patents 2000, 10, 575-600. 
Competing interests:
None declared.
Please review this paper.
user
Reviewer's report
 The plasma membrane carbonic anhydrase in murine hepatocytes identified as isozyme XIV Title:
Authors:
Seppo Parkkila (seppo.parkkila@uta.fi )
Antti J Kivela (akivela@paju.oulu.fi )
Kari Kaunisto (kari.kaunisto@oulu.fi )
Anna-Kaisa Parkkila (akparkkila@hotmail.com )
Jukka Hakkola (jukka.hakkola@oulu.fi )
Hannu Rajaniemi (hannu.rajaniemi@oulu.fi )
Abdul Waheed (waheeda@slu.edu )
William S Sly (slyws@slu.edu )
 1  8 May 2002 Version: Date:
 Claudiu Supuran Reviewer:
 not specified Level of interest:
 Accept after discretionary revisions Advice on publication:
Recommendation: PUBLISH with minor revision 
Comments: This is an interesting work regarding the identification of the isozyme type responsible for 
CA activity in the hepatocyte plasma membranes. Up to now it was considered that this is due to 
iszoyme CA IV, but the present authors clearly show that this is due to the recently isoalted isozyme CA 
XIV. The experimental techniques described are straightforward and properly described. 
Furtermore: 
1. Are the conclusions drawn adequately supported by the data shown: 
Yes, they are. 
2. Are sufficcient details provided to allow replication of the work or comparison with related analyses: 
Yes. 
3. Does the manuscript adhere to the relevant standards for reporting and data deposition: 
yes. 
4. Is the writing acceptable? 
No, references provided are insufficient. For example, in several recent reviews, the cellular distribution 
and physiological functions of the various CA isozymes have been extensively discussed. These works 
must be cited, such as for example: C.T. Supuran, A. Scozzafava, Exp. Opin. Ther. Patents 2002, 12, 217-242. 
C.T. Supuran, A. Scozzafava, Exp. Opin. Ther. Patents 2000, 10, 575-600. 
Competing interests:
None declared.
assistant