Reviewer's report
Title:Glucagon-like peptides 1 and 2 and vasoactive intestinal peptide are
neuroprotective on cultured and mast cell co-cultured rat myenteric neurons
Version: 1Date:19 December 2011
Reviewer: Marcin Arciszewski
Reviewer's report:
The study of E. Ekblad group is well designed and the manuscript is written in
understandable manner. The aims, methods and the results are clearly
described. The discussion is broad and adequate.
Discretionary Revisions
The authors declared that two neuronal markers (PGP 9.5 and Hu C/D) were
used in the study. However, Fig 1 presents cultured myenteric neurons stained
with PGP 9.5 anti-sera only (no Hu C/D-positive cultured perikarya were
presented) which makes an impression that PGP 9.5 neuronal marker was
predominantly used. If the latter is true it is not clear in which circumstances the
authors decided to use Hu C/D antibodies. Moreover, it is not clear whether any
changes in percentages of survived neurons were noted when analogous
neuronal cultures were stained with different neuronal markers (Hu C/D vs. PGP
9.5). This aspect may be of importance since PGP 9.5 was found to be
expressed in cultured fibroblast (Olerud et al.; J Inv. Derm, 1998). Therefore,
there is a low risk that a few PGP-positive cultured fibroblast (which are known to
be present in myenteric neuronal cultures) could be counted as cultured neurons.
Level of interest: An article of outstanding merit and interest in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Glucagon-like peptides 1 and 2 and vasoactive intestinal peptide are
neuroprotective on cultured and mast cell co-cultured rat myenteric neurons
Version: 1Date:19 December 2011
Reviewer: Marcin Arciszewski
Reviewer's report:
The study of E. Ekblad group is well designed and the manuscript is written in
understandable manner. The aims, methods and the results are clearly
described. The discussion is broad and adequate.
Discretionary Revisions
The authors declared that two neuronal markers (PGP 9.5 and Hu C/D) were
used in the study. However, Fig 1 presents cultured myenteric neurons stained
with PGP 9.5 anti-sera only (no Hu C/D-positive cultured perikarya were
presented) which makes an impression that PGP 9.5 neuronal marker was
predominantly used. If the latter is true it is not clear in which circumstances the
authors decided to use Hu C/D antibodies. Moreover, it is not clear whether any
changes in percentages of survived neurons were noted when analogous
neuronal cultures were stained with different neuronal markers (Hu C/D vs. PGP
9.5). This aspect may be of importance since PGP 9.5 was found to be
expressed in cultured fibroblast (Olerud et al.; J Inv. Derm, 1998). Therefore,
there is a low risk that a few PGP-positive cultured fibroblast (which are known to
be present in myenteric neuronal cultures) could be counted as cultured neurons.
Level of interest: An article of outstanding merit and interest in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
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Reviewer's report
Title:Glucagon-like peptides 1 and 2 and vasoactive intestinal peptide are
neuroprotective on cultured and mast cell co-cultured rat myenteric neurons
Version: 1Date:19 December 2011
Reviewer: Marcin Arciszewski
Reviewer's report:
The study of E. Ekblad group is well designed and the manuscript is written in
understandable manner. The aims, methods and the results are clearly
described. The discussion is broad and adequate.
Discretionary Revisions
The authors declared that two neuronal markers (PGP 9.5 and Hu C/D) were
used in the study. However, Fig 1 presents cultured myenteric neurons stained
with PGP 9.5 anti-sera only (no Hu C/D-positive cultured perikarya were
presented) which makes an impression that PGP 9.5 neuronal marker was
predominantly used. If the latter is true it is not clear in which circumstances the
authors decided to use Hu C/D antibodies. Moreover, it is not clear whether any
changes in percentages of survived neurons were noted when analogous
neuronal cultures were stained with different neuronal markers (Hu C/D vs. PGP
9.5). This aspect may be of importance since PGP 9.5 was found to be
expressed in cultured fibroblast (Olerud et al.; J Inv. Derm, 1998). Therefore,
there is a low risk that a few PGP-positive cultured fibroblast (which are known to
be present in myenteric neuronal cultures) could be counted as cultured neurons.
Level of interest: An article of outstanding merit and interest in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
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