Reviewer's report
Title:Prevalence of Genetic Polymorphisms in the Promoter Region of the
Alpha-1 Antitrypsin (SERPINA1) Gene in Chronic Liver Disease: a Case Control
Study
Version: 3Date:18 December 2009
Reviewer: Sally Chappell
Reviewer's report:
The authors have addressed the majority of comments raised during the review
process. The replacement of table 2 is a vast improvement on the previous
version, although I would still like to see a little more detail on how the
diplotypes/haplotypes were constructed. Was a software package (eg HPlus,
Haploview, Unphased) used for this analysis? If so, more detail needs to be
given. Where individuals are either homozygous at all of the polymorphic sites, or
only heterozygous at one site, it is straightforward to determine the haplotypes.
However, this is more difficult with heterozygosity at 2 or more sites, and I would
be interested to know how this was done. Values for linkage disequilibrium (r2)
would also be of interest, and could be added here for the controls and cases
separately.
Level of interest: An article of limited interest
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Prevalence of Genetic Polymorphisms in the Promoter Region of the
Alpha-1 Antitrypsin (SERPINA1) Gene in Chronic Liver Disease: a Case Control
Study
Version: 3Date:18 December 2009
Reviewer: Sally Chappell
Reviewer's report:
The authors have addressed the majority of comments raised during the review
process. The replacement of table 2 is a vast improvement on the previous
version, although I would still like to see a little more detail on how the
diplotypes/haplotypes were constructed. Was a software package (eg HPlus,
Haploview, Unphased) used for this analysis? If so, more detail needs to be
given. Where individuals are either homozygous at all of the polymorphic sites, or
only heterozygous at one site, it is straightforward to determine the haplotypes.
However, this is more difficult with heterozygosity at 2 or more sites, and I would
be interested to know how this was done. Values for linkage disequilibrium (r2)
would also be of interest, and could be added here for the controls and cases
separately.
Level of interest: An article of limited interest
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Prevalence of Genetic Polymorphisms in the Promoter Region of the
Alpha-1 Antitrypsin (SERPINA1) Gene in Chronic Liver Disease: a Case Control
Study
Version: 3Date:18 December 2009
Reviewer: Sally Chappell
Reviewer's report:
The authors have addressed the majority of comments raised during the review
process. The replacement of table 2 is a vast improvement on the previous
version, although I would still like to see a little more detail on how the
diplotypes/haplotypes were constructed. Was a software package (eg HPlus,
Haploview, Unphased) used for this analysis? If so, more detail needs to be
given. Where individuals are either homozygous at all of the polymorphic sites, or
only heterozygous at one site, it is straightforward to determine the haplotypes.
However, this is more difficult with heterozygosity at 2 or more sites, and I would
be interested to know how this was done. Values for linkage disequilibrium (r2)
would also be of interest, and could be added here for the controls and cases
separately.
Level of interest: An article of limited interest
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
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