Title: An Alternative Splicing Signature in Human Crohn’s Disease
Version: 2 Date: 03 Jun 2021
Reviewer's report:
In the validation study, the authors sequenced 8 subjects. The sample size is really small which
made the conclusion is not so robust. The included CD patients all received different medications
including biologics and immunosuppression which may also infect the expression of genes.
Better to include treatment naïve CD patients.
Among the discovered approximately 3,000 unique AS events in CD patients compared to the
healthy controls from a public mRNA-seq data, the authors only validated two AS events which
are STAT3 and IRF1 genes. How about the other genes? Moreover, whether these identified AS
events play a vital role in the pathogenesis of CD, eg, affecting protein diversity or downstream
signaling transduction pathways warrant further study.
There are some grammatical and spelling errors in this manuscript which need to be further
proof-reading by native speakers.
Title: An Alternative Splicing Signature in Human Crohn’s Disease
Version: 2 Date: 03 Jun 2021
Reviewer's report:
In the validation study, the authors sequenced 8 subjects. The sample size is really small which
made the conclusion is not so robust. The included CD patients all received different medications
including biologics and immunosuppression which may also infect the expression of genes.
Better to include treatment naïve CD patients.
Among the discovered approximately 3,000 unique AS events in CD patients compared to the
healthy controls from a public mRNA-seq data, the authors only validated two AS events which
are STAT3 and IRF1 genes. How about the other genes? Moreover, whether these identified AS
events play a vital role in the pathogenesis of CD, eg, affecting protein diversity or downstream
signaling transduction pathways warrant further study.
There are some grammatical and spelling errors in this manuscript which need to be further
proof-reading by native speakers.
Please review this paper.
user
Title: An Alternative Splicing Signature in Human Crohn’s Disease
Version: 2 Date: 03 Jun 2021
Reviewer's report:
In the validation study, the authors sequenced 8 subjects. The sample size is really small which
made the conclusion is not so robust. The included CD patients all received different medications
including biologics and immunosuppression which may also infect the expression of genes.
Better to include treatment naïve CD patients.
Among the discovered approximately 3,000 unique AS events in CD patients compared to the
healthy controls from a public mRNA-seq data, the authors only validated two AS events which
are STAT3 and IRF1 genes. How about the other genes? Moreover, whether these identified AS
events play a vital role in the pathogenesis of CD, eg, affecting protein diversity or downstream
signaling transduction pathways warrant further study.
There are some grammatical and spelling errors in this manuscript which need to be further
proof-reading by native speakers.
assistant