Title: Excess fatty acids induce pancreatic acinar cell pyroptosis through macrophage M1
polarization
Version: 0 Date: 29 Sep 2021
Reviewer:  Undurti N. Das
Reviewer's report:
Well conducted study. But the manuscript need to be reorganized.
1. First describe the in vitro studies, then animal study and finally clinical study. By combining
all the studies in a random fashion it is rather confusing and logical sequence of study is lost.
2. Describe the in vitro studies and form a hypothesis or logical argument as to what you wish
to do in an in vivo study and why.
3. The describe the animal studies with clear hypothesis and form arguments as to how they
have been verified and how the results obtained are in support of the same.
4. Finally describe how the in vitro and animal study results have been verified are supported
by the human studies.
This logical sequence of arrangement of the manuscript is needed to make it to make it more
appealing. In the same sequence even the experimental procedures also need to be arranged. The
same sequence need to be followed to describe results and discussion sections as well.
5. I wonder why plasma levels of cytokines were not measured in patients with pancreatitis to
reestablish wat they have obtained in the animal model.
6. Assuming that hyperlipidemia occurs or precedes the occurrence of pancreatitis, why plasma
levels of unsaturated fatty acids was not measured. Is it possible that palmitic acid induced M1
generation can be prevented by unsaturated fatty acids?
7. Does this mean unsaturated fatty acids infusion is of help to those with acute pancreatitis.
8. If the model developed/used by the authors in the animals is right, can the pancreatitis be
reversed by u=suitable intervention(s). If so, what measrues or interventions the authors propose
9. I would appreciate if the manuscript is complete with all the figures as one file instead of all
the figures being submitted or sent to the reviewer separately.
Title: Excess fatty acids induce pancreatic acinar cell pyroptosis through macrophage M1
polarization
Version: 0 Date: 29 Sep 2021
Reviewer:  Undurti N. Das
Reviewer's report:
Well conducted study. But the manuscript need to be reorganized.
1. First describe the in vitro studies, then animal study and finally clinical study. By combining
all the studies in a random fashion it is rather confusing and logical sequence of study is lost.
2. Describe the in vitro studies and form a hypothesis or logical argument as to what you wish
to do in an in vivo study and why.
3. The describe the animal studies with clear hypothesis and form arguments as to how they
have been verified and how the results obtained are in support of the same.
4. Finally describe how the in vitro and animal study results have been verified are supported
by the human studies.
This logical sequence of arrangement of the manuscript is needed to make it to make it more
appealing. In the same sequence even the experimental procedures also need to be arranged. The
same sequence need to be followed to describe results and discussion sections as well.
5. I wonder why plasma levels of cytokines were not measured in patients with pancreatitis to
reestablish wat they have obtained in the animal model.
6. Assuming that hyperlipidemia occurs or precedes the occurrence of pancreatitis, why plasma
levels of unsaturated fatty acids was not measured. Is it possible that palmitic acid induced M1
generation can be prevented by unsaturated fatty acids?
7. Does this mean unsaturated fatty acids infusion is of help to those with acute pancreatitis.
8. If the model developed/used by the authors in the animals is right, can the pancreatitis be
reversed by u=suitable intervention(s). If so, what measrues or interventions the authors propose
9. I would appreciate if the manuscript is complete with all the figures as one file instead of all
the figures being submitted or sent to the reviewer separately.
Please review this paper.
user
Title: Excess fatty acids induce pancreatic acinar cell pyroptosis through macrophage M1
polarization
Version: 0 Date: 29 Sep 2021
Reviewer:  Undurti N. Das
Reviewer's report:
Well conducted study. But the manuscript need to be reorganized.
1. First describe the in vitro studies, then animal study and finally clinical study. By combining
all the studies in a random fashion it is rather confusing and logical sequence of study is lost.
2. Describe the in vitro studies and form a hypothesis or logical argument as to what you wish
to do in an in vivo study and why.
3. The describe the animal studies with clear hypothesis and form arguments as to how they
have been verified and how the results obtained are in support of the same.
4. Finally describe how the in vitro and animal study results have been verified are supported
by the human studies.
This logical sequence of arrangement of the manuscript is needed to make it to make it more
appealing. In the same sequence even the experimental procedures also need to be arranged. The
same sequence need to be followed to describe results and discussion sections as well.
5. I wonder why plasma levels of cytokines were not measured in patients with pancreatitis to
reestablish wat they have obtained in the animal model.
6. Assuming that hyperlipidemia occurs or precedes the occurrence of pancreatitis, why plasma
levels of unsaturated fatty acids was not measured. Is it possible that palmitic acid induced M1
generation can be prevented by unsaturated fatty acids?
7. Does this mean unsaturated fatty acids infusion is of help to those with acute pancreatitis.
8. If the model developed/used by the authors in the animals is right, can the pancreatitis be
reversed by u=suitable intervention(s). If so, what measrues or interventions the authors propose
9. I would appreciate if the manuscript is complete with all the figures as one file instead of all
the figures being submitted or sent to the reviewer separately.
assistant