Reviewer's report
Title:Intestinal Epithelial Serum Amyloid A Modulates Bacterial Growth and
Protects from Experimental Colitis
Version: 1Date:22 September 2010
Reviewer: Aldo AM Lima
Reviewer's report:
* Major Compulsory Revisions
This is an interesting paper showing the modulation of SAA on E. coli in vitro
growth and pro-inflammatory response of experimental colitis in a mouse model.
Major concern on this paper is how to fit the clinical results on the finding from
the in vitro and in vivo results. As showed in the clinical results, the cases
(Crohn's diseases) increased the SAA1/2 RNA expressions with the diseases
which are against the controling of inflammation compared to control health
volunteers. Since the in vivo model are not close comparable to clinical disease, I
would suggest to delete this part without prejudice to the whole nice work. I agree
that more work will be necessary to explain the modulation of SAA in Crohn's
diseases and the authors stated that in the discussion.
* Minor Essential Revisions
Some minor points:
1. Suggested for new title: Intestinal epithelial serum amyloid A modulates
bacterial growth in vitro and pro-inflammatory responses in mouse experimental
colitis;
2. At some how I felt hard to follow the abstract before read the whole paper.
Please revise saying the three isoforms of SAA/add if all forms types of
IECs/delete result clinical results since no conclusion could be make using these
results;
3. Add a few more keywords would help;
4. What is the nomeclature denomination for gene and protein for SAA?;
5. on methods 2nd paragraphic please spell the letters "OCT";
6. Results, subitem SAA protects against DSS colitits; please revise if lost weight
was statistical significant since it is not clear there or in the correspondent Fig. 5;
7.Do the authors have a chance to add IEC cells with internalized E. coli?;
8. Figure 1 was hard to follow as described without saying what we should look in
term of colors etc; specially Fig1 F and it maybe good to add arrows to show
better those points.
* Discretionary Revisions
Delete clinical results.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:Intestinal Epithelial Serum Amyloid A Modulates Bacterial Growth and
Protects from Experimental Colitis
Version: 1Date:22 September 2010
Reviewer: Aldo AM Lima
Reviewer's report:
* Major Compulsory Revisions
This is an interesting paper showing the modulation of SAA on E. coli in vitro
growth and pro-inflammatory response of experimental colitis in a mouse model.
Major concern on this paper is how to fit the clinical results on the finding from
the in vitro and in vivo results. As showed in the clinical results, the cases
(Crohn's diseases) increased the SAA1/2 RNA expressions with the diseases
which are against the controling of inflammation compared to control health
volunteers. Since the in vivo model are not close comparable to clinical disease, I
would suggest to delete this part without prejudice to the whole nice work. I agree
that more work will be necessary to explain the modulation of SAA in Crohn's
diseases and the authors stated that in the discussion.
* Minor Essential Revisions
Some minor points:
1. Suggested for new title: Intestinal epithelial serum amyloid A modulates
bacterial growth in vitro and pro-inflammatory responses in mouse experimental
colitis;
2. At some how I felt hard to follow the abstract before read the whole paper.
Please revise saying the three isoforms of SAA/add if all forms types of
IECs/delete result clinical results since no conclusion could be make using these
results;
3. Add a few more keywords would help;
4. What is the nomeclature denomination for gene and protein for SAA?;
5. on methods 2nd paragraphic please spell the letters "OCT";
6. Results, subitem SAA protects against DSS colitits; please revise if lost weight
was statistical significant since it is not clear there or in the correspondent Fig. 5;
7.Do the authors have a chance to add IEC cells with internalized E. coli?;
8. Figure 1 was hard to follow as described without saying what we should look in
term of colors etc; specially Fig1 F and it maybe good to add arrows to show
better those points.
* Discretionary Revisions
Delete clinical results.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:Intestinal Epithelial Serum Amyloid A Modulates Bacterial Growth and
Protects from Experimental Colitis
Version: 1Date:22 September 2010
Reviewer: Aldo AM Lima
Reviewer's report:
* Major Compulsory Revisions
This is an interesting paper showing the modulation of SAA on E. coli in vitro
growth and pro-inflammatory response of experimental colitis in a mouse model.
Major concern on this paper is how to fit the clinical results on the finding from
the in vitro and in vivo results. As showed in the clinical results, the cases
(Crohn's diseases) increased the SAA1/2 RNA expressions with the diseases
which are against the controling of inflammation compared to control health
volunteers. Since the in vivo model are not close comparable to clinical disease, I
would suggest to delete this part without prejudice to the whole nice work. I agree
that more work will be necessary to explain the modulation of SAA in Crohn's
diseases and the authors stated that in the discussion.
* Minor Essential Revisions
Some minor points:
1. Suggested for new title: Intestinal epithelial serum amyloid A modulates
bacterial growth in vitro and pro-inflammatory responses in mouse experimental
colitis;
2. At some how I felt hard to follow the abstract before read the whole paper.
Please revise saying the three isoforms of SAA/add if all forms types of
IECs/delete result clinical results since no conclusion could be make using these
results;
3. Add a few more keywords would help;
4. What is the nomeclature denomination for gene and protein for SAA?;
5. on methods 2nd paragraphic please spell the letters "OCT";
6. Results, subitem SAA protects against DSS colitits; please revise if lost weight
was statistical significant since it is not clear there or in the correspondent Fig. 5;
7.Do the authors have a chance to add IEC cells with internalized E. coli?;
8. Figure 1 was hard to follow as described without saying what we should look in
term of colors etc; specially Fig1 F and it maybe good to add arrows to show
better those points.
* Discretionary Revisions
Delete clinical results.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
assistant