Title: Colonic distribution of FMT by different enema procedures compared to colonoscopy –
proof of concept study using contrast fluid
Version: 3 Date: 03 Aug 2023
Reviewer:  Zhi-Ning Ye
Reviewer's report:
Strengths and limitations
The topic of this study is interesting, as it firstly evaluated how fluid similar to FMT is distributed
in the colon after delivery by colonoscopy or enema. It is important for the in-depth study of the
therapeutic mechanism of FMT on various diseases. However, the generalizability of the study
is greatly reduced by the limitations of the study's sample size and observation outcomes.
Results:
1.The study incorporated a sample that was predominantly older. As colonic motility may vary
across different age groups, it would be better to discuss the potential effect of age on the study's
observed outcomes.
2.The authors should use the grayscale of the pictures as an evaluation outcome if possible.
Discussion:
1.Another potential limitation of this study is the absence of a longitudinal investigation into the
diffusion of CF. Such an investigation may have imparted additional significance to the findings.
Title: Colonic distribution of FMT by different enema procedures compared to colonoscopy –
proof of concept study using contrast fluid
Version: 3 Date: 03 Aug 2023
Reviewer:  Zhi-Ning Ye
Reviewer's report:
Strengths and limitations
The topic of this study is interesting, as it firstly evaluated how fluid similar to FMT is distributed
in the colon after delivery by colonoscopy or enema. It is important for the in-depth study of the
therapeutic mechanism of FMT on various diseases. However, the generalizability of the study
is greatly reduced by the limitations of the study's sample size and observation outcomes.
Results:
1.The study incorporated a sample that was predominantly older. As colonic motility may vary
across different age groups, it would be better to discuss the potential effect of age on the study's
observed outcomes.
2.The authors should use the grayscale of the pictures as an evaluation outcome if possible.
Discussion:
1.Another potential limitation of this study is the absence of a longitudinal investigation into the
diffusion of CF. Such an investigation may have imparted additional significance to the findings.
Please review this paper.
user
Title: Colonic distribution of FMT by different enema procedures compared to colonoscopy –
proof of concept study using contrast fluid
Version: 3 Date: 03 Aug 2023
Reviewer:  Zhi-Ning Ye
Reviewer's report:
Strengths and limitations
The topic of this study is interesting, as it firstly evaluated how fluid similar to FMT is distributed
in the colon after delivery by colonoscopy or enema. It is important for the in-depth study of the
therapeutic mechanism of FMT on various diseases. However, the generalizability of the study
is greatly reduced by the limitations of the study's sample size and observation outcomes.
Results:
1.The study incorporated a sample that was predominantly older. As colonic motility may vary
across different age groups, it would be better to discuss the potential effect of age on the study's
observed outcomes.
2.The authors should use the grayscale of the pictures as an evaluation outcome if possible.
Discussion:
1.Another potential limitation of this study is the absence of a longitudinal investigation into the
diffusion of CF. Such an investigation may have imparted additional significance to the findings.
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