Title: Intestinal Claudin-7 deficiency impacts the intestinal microbiota in mice with colitis
Version: 0 Date: 19 May 2021
Reviewer's report:
In previous research of this lab, non-tight junction effects of claudin-7 deficiency have been
characterized. The present study continues on this topic by examining claudin-7 deficiency in
a DSS-induced colitis mouse model.
It is shown here that in DSS-treated mice the Cldn7-deficiency causes (i) the intestinal damage to
become worse, (ii) the mucus barrier being impaired which may increase the access to bacteria,
(iii) CreERT2 mice having a greater reduction in intestinal microbial diversity than CreW mice,
and (iv) phylum proteobacteria E. Shigella being the most significant species in CreERT2 mice.
Specific points:
In the Abstract as well as in the Conclusions it is claimed that the results of this study provide
a new basis for IBD treatment, but no idea how this should work was given. Therefore, this
statement should be removed at both sites.
Fig. 3: Letterings are too small, all lines too thin.
Fig. 4: All parts of the Figure are too small.
Title: Intestinal Claudin-7 deficiency impacts the intestinal microbiota in mice with colitis
Version: 0 Date: 19 May 2021
Reviewer's report:
In previous research of this lab, non-tight junction effects of claudin-7 deficiency have been
characterized. The present study continues on this topic by examining claudin-7 deficiency in
a DSS-induced colitis mouse model.
It is shown here that in DSS-treated mice the Cldn7-deficiency causes (i) the intestinal damage to
become worse, (ii) the mucus barrier being impaired which may increase the access to bacteria,
(iii) CreERT2 mice having a greater reduction in intestinal microbial diversity than CreW mice,
and (iv) phylum proteobacteria E. Shigella being the most significant species in CreERT2 mice.
Specific points:
In the Abstract as well as in the Conclusions it is claimed that the results of this study provide
a new basis for IBD treatment, but no idea how this should work was given. Therefore, this
statement should be removed at both sites.
Fig. 3: Letterings are too small, all lines too thin.
Fig. 4: All parts of the Figure are too small.
Please review this paper.
user
Title: Intestinal Claudin-7 deficiency impacts the intestinal microbiota in mice with colitis
Version: 0 Date: 19 May 2021
Reviewer's report:
In previous research of this lab, non-tight junction effects of claudin-7 deficiency have been
characterized. The present study continues on this topic by examining claudin-7 deficiency in
a DSS-induced colitis mouse model.
It is shown here that in DSS-treated mice the Cldn7-deficiency causes (i) the intestinal damage to
become worse, (ii) the mucus barrier being impaired which may increase the access to bacteria,
(iii) CreERT2 mice having a greater reduction in intestinal microbial diversity than CreW mice,
and (iv) phylum proteobacteria E. Shigella being the most significant species in CreERT2 mice.
Specific points:
In the Abstract as well as in the Conclusions it is claimed that the results of this study provide
a new basis for IBD treatment, but no idea how this should work was given. Therefore, this
statement should be removed at both sites.
Fig. 3: Letterings are too small, all lines too thin.
Fig. 4: All parts of the Figure are too small.
assistant