Reviewer's report
Title:Carbon monoxide-Releasing Molecule-2 (CORM-2) attenuates acute
hepatic ischemia reperfusion injury in rats
Version: 1Date:20 January 2010
Reviewer: Sarah Hosgood
Reviewer's report:
Major compulsory revisions
1: This manuscript presents very clear and concise results. However, I suggest
that more detail given to the statistical analysis. The Kruskal-Wallis test was used
to compared groups. I assume that the P value stated in the legends (<0.05)
related to the post test? I think a more detailed explanation of the statistics and
inclusion of the P value in the text of the results section is needed.
2: The first paragraph of the discussion states that CORM-2 reduced
ROS-mediated hepatic ischemia reperfusion injury. This is not completely
accurate as no measures of oxidative damage were performed.
Minor essential revisions
1: In figure 3b, is the level of caspase 3 significantly lower in the CORM 2 group?
The * is missing
Discretionary Revisions
1: Measures of oxidatived damaged would strengthen this manuscript, however I
do not feel that this is essential given the substantial range of parameters
measured.
2: I assume that all rats survived during the 6 hour reperfusion perfusion period?
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Carbon monoxide-Releasing Molecule-2 (CORM-2) attenuates acute
hepatic ischemia reperfusion injury in rats
Version: 1Date:20 January 2010
Reviewer: Sarah Hosgood
Reviewer's report:
Major compulsory revisions
1: This manuscript presents very clear and concise results. However, I suggest
that more detail given to the statistical analysis. The Kruskal-Wallis test was used
to compared groups. I assume that the P value stated in the legends (<0.05)
related to the post test? I think a more detailed explanation of the statistics and
inclusion of the P value in the text of the results section is needed.
2: The first paragraph of the discussion states that CORM-2 reduced
ROS-mediated hepatic ischemia reperfusion injury. This is not completely
accurate as no measures of oxidative damage were performed.
Minor essential revisions
1: In figure 3b, is the level of caspase 3 significantly lower in the CORM 2 group?
The * is missing
Discretionary Revisions
1: Measures of oxidatived damaged would strengthen this manuscript, however I
do not feel that this is essential given the substantial range of parameters
measured.
2: I assume that all rats survived during the 6 hour reperfusion perfusion period?
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Carbon monoxide-Releasing Molecule-2 (CORM-2) attenuates acute
hepatic ischemia reperfusion injury in rats
Version: 1Date:20 January 2010
Reviewer: Sarah Hosgood
Reviewer's report:
Major compulsory revisions
1: This manuscript presents very clear and concise results. However, I suggest
that more detail given to the statistical analysis. The Kruskal-Wallis test was used
to compared groups. I assume that the P value stated in the legends (<0.05)
related to the post test? I think a more detailed explanation of the statistics and
inclusion of the P value in the text of the results section is needed.
2: The first paragraph of the discussion states that CORM-2 reduced
ROS-mediated hepatic ischemia reperfusion injury. This is not completely
accurate as no measures of oxidative damage were performed.
Minor essential revisions
1: In figure 3b, is the level of caspase 3 significantly lower in the CORM 2 group?
The * is missing
Discretionary Revisions
1: Measures of oxidatived damaged would strengthen this manuscript, however I
do not feel that this is essential given the substantial range of parameters
measured.
2: I assume that all rats survived during the 6 hour reperfusion perfusion period?
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
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