Reviewer's report
Title:Growth Inhibition and Apoptosis Induced by 6-fluoro-3-formylchromone in
Hepatocellular Carcinoma
Version:1Date:2 January 2014
Reviewer: Tyvin A A Rich
Reviewer's report:
In this manuscript, the effects of 6-fluoro-3-formylchromone on hepatocellular
carcinoma cell line SMMC-7721 were investigated. The results indicated that
6-fluoro-3-formylchromone could significantly inhibit hepatocellular carcinoma cell
growth through cell cycle arrest and inducing apoptosis by suppressing PCNA
expression and modulating the Bax/Bcl-2 ratio. Here are my suggestions for
minor revision:
1 The abstract of this manuscript should be written in more details, such as the
studied concentrations and statitical analysis results.
2 In this study, the expression levels of PCNA, Bax and Bcl-2 in SMMC-7721
cells incubated with 6-fluoro-3-formylchromone for 48 h were measured. The
importance of these three indexes might be better described briefly in the
introduction.
3 Only the chemical structure of 6-fluoro-3-formylchromone was shown in Figure
1A and the characteristics of this substance could be introduced.
4 The cells were treated with 0, 2, 5, 10, and 20 µg/ml
6-fluoro-3-formylchromone. Do 0, 2, 5, 10, and 20 µg/ml mean the final
concentrations of cell solution or the concentrations of 6-fluoro-3-formylchromone
solution? If the latter, please give the volume of the 6-fluoro-3-formylchromone
solution.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Growth Inhibition and Apoptosis Induced by 6-fluoro-3-formylchromone in
Hepatocellular Carcinoma
Version:1Date:2 January 2014
Reviewer: Tyvin A A Rich
Reviewer's report:
In this manuscript, the effects of 6-fluoro-3-formylchromone on hepatocellular
carcinoma cell line SMMC-7721 were investigated. The results indicated that
6-fluoro-3-formylchromone could significantly inhibit hepatocellular carcinoma cell
growth through cell cycle arrest and inducing apoptosis by suppressing PCNA
expression and modulating the Bax/Bcl-2 ratio. Here are my suggestions for
minor revision:
1 The abstract of this manuscript should be written in more details, such as the
studied concentrations and statitical analysis results.
2 In this study, the expression levels of PCNA, Bax and Bcl-2 in SMMC-7721
cells incubated with 6-fluoro-3-formylchromone for 48 h were measured. The
importance of these three indexes might be better described briefly in the
introduction.
3 Only the chemical structure of 6-fluoro-3-formylchromone was shown in Figure
1A and the characteristics of this substance could be introduced.
4 The cells were treated with 0, 2, 5, 10, and 20 µg/ml
6-fluoro-3-formylchromone. Do 0, 2, 5, 10, and 20 µg/ml mean the final
concentrations of cell solution or the concentrations of 6-fluoro-3-formylchromone
solution? If the latter, please give the volume of the 6-fluoro-3-formylchromone
solution.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Growth Inhibition and Apoptosis Induced by 6-fluoro-3-formylchromone in
Hepatocellular Carcinoma
Version:1Date:2 January 2014
Reviewer: Tyvin A A Rich
Reviewer's report:
In this manuscript, the effects of 6-fluoro-3-formylchromone on hepatocellular
carcinoma cell line SMMC-7721 were investigated. The results indicated that
6-fluoro-3-formylchromone could significantly inhibit hepatocellular carcinoma cell
growth through cell cycle arrest and inducing apoptosis by suppressing PCNA
expression and modulating the Bax/Bcl-2 ratio. Here are my suggestions for
minor revision:
1 The abstract of this manuscript should be written in more details, such as the
studied concentrations and statitical analysis results.
2 In this study, the expression levels of PCNA, Bax and Bcl-2 in SMMC-7721
cells incubated with 6-fluoro-3-formylchromone for 48 h were measured. The
importance of these three indexes might be better described briefly in the
introduction.
3 Only the chemical structure of 6-fluoro-3-formylchromone was shown in Figure
1A and the characteristics of this substance could be introduced.
4 The cells were treated with 0, 2, 5, 10, and 20 µg/ml
6-fluoro-3-formylchromone. Do 0, 2, 5, 10, and 20 µg/ml mean the final
concentrations of cell solution or the concentrations of 6-fluoro-3-formylchromone
solution? If the latter, please give the volume of the 6-fluoro-3-formylchromone
solution.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I declare that I have no competing interests
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