Reviewer's report
Title:Requirement of epithelial integrin-linked kinase for facilitation of Citrobacter
rodentium-induced colitis.
Version: 1Date:7 April 2013
Reviewer: Shahid Umar
Reviewer's report:
Integrin-linked kinases (ILK) have been shown previously to be involved in
bacterial infection-induced stabilization of focal adhesion and cell attachment. In
the study presented here, the authors examine ILK's role in the pathogenesis of
Citrobacter rodentium infection and provide a possible therapeutic target for
studying diseases with infectious etiology. The manuscript is well written for the
most part and provides significant amount of data to justify ILK's role in bacterial
infection-induced hyperplastic and/or inflammatory responses. The manuscript
may be further improved if following modifications are made:
1. It will be helpful if half-life of ILK in various cell lines is atleast discussed if not
experimentally proven.
2. The way the results are interpreted for Figure 1 suggests that somehow total
Akt has to be affected in response to ILK activation, giving less significance to
the phosphorylated form of the protein which is more important.
3. Please provide ILK levels in Figure 1B to show its knockdown following siRNA
treatment.
4. Likewise, ILK levels should be shown for comparison in Figure 5. It may be
beyond the scope of this paper but the manuscript will improve significantly if
epithelial cells instead of whole tissue lysate is utilized for protein measurement
as doing this will confirm ILK expression in the crypt given that the IHC shown for
ILK in Figure 2E is not very conclusive.
Level of interest: An article of outstanding merit and interest in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
'I declare that I have no competing interests'
Reviewer's report
Title:Requirement of epithelial integrin-linked kinase for facilitation of Citrobacter
rodentium-induced colitis.
Version: 1Date:7 April 2013
Reviewer: Shahid Umar
Reviewer's report:
Integrin-linked kinases (ILK) have been shown previously to be involved in
bacterial infection-induced stabilization of focal adhesion and cell attachment. In
the study presented here, the authors examine ILK's role in the pathogenesis of
Citrobacter rodentium infection and provide a possible therapeutic target for
studying diseases with infectious etiology. The manuscript is well written for the
most part and provides significant amount of data to justify ILK's role in bacterial
infection-induced hyperplastic and/or inflammatory responses. The manuscript
may be further improved if following modifications are made:
1. It will be helpful if half-life of ILK in various cell lines is atleast discussed if not
experimentally proven.
2. The way the results are interpreted for Figure 1 suggests that somehow total
Akt has to be affected in response to ILK activation, giving less significance to
the phosphorylated form of the protein which is more important.
3. Please provide ILK levels in Figure 1B to show its knockdown following siRNA
treatment.
4. Likewise, ILK levels should be shown for comparison in Figure 5. It may be
beyond the scope of this paper but the manuscript will improve significantly if
epithelial cells instead of whole tissue lysate is utilized for protein measurement
as doing this will confirm ILK expression in the crypt given that the IHC shown for
ILK in Figure 2E is not very conclusive.
Level of interest: An article of outstanding merit and interest in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
'I declare that I have no competing interests'
Please review this paper.
user
Reviewer's report
Title:Requirement of epithelial integrin-linked kinase for facilitation of Citrobacter
rodentium-induced colitis.
Version: 1Date:7 April 2013
Reviewer: Shahid Umar
Reviewer's report:
Integrin-linked kinases (ILK) have been shown previously to be involved in
bacterial infection-induced stabilization of focal adhesion and cell attachment. In
the study presented here, the authors examine ILK's role in the pathogenesis of
Citrobacter rodentium infection and provide a possible therapeutic target for
studying diseases with infectious etiology. The manuscript is well written for the
most part and provides significant amount of data to justify ILK's role in bacterial
infection-induced hyperplastic and/or inflammatory responses. The manuscript
may be further improved if following modifications are made:
1. It will be helpful if half-life of ILK in various cell lines is atleast discussed if not
experimentally proven.
2. The way the results are interpreted for Figure 1 suggests that somehow total
Akt has to be affected in response to ILK activation, giving less significance to
the phosphorylated form of the protein which is more important.
3. Please provide ILK levels in Figure 1B to show its knockdown following siRNA
treatment.
4. Likewise, ILK levels should be shown for comparison in Figure 5. It may be
beyond the scope of this paper but the manuscript will improve significantly if
epithelial cells instead of whole tissue lysate is utilized for protein measurement
as doing this will confirm ILK expression in the crypt given that the IHC shown for
ILK in Figure 2E is not very conclusive.
Level of interest: An article of outstanding merit and interest in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
'I declare that I have no competing interests'
assistant