Reviewer's report
Title:NOX2-generated oxidative stress is associated with severity of ultrasound
liver steatosis in patients with non-alcoholic fatty liver disease
Version: 3Date:8 October 2013
Reviewer: Begoña Ochoa
Reviewer's report:
Given that few studies have reported increased circulating levels of oxidative
stress markers in patients with non-alcoholic fatty liver and no published study
has been performed with newer markers of systemic oxidative stress, the authors
aim to assess the relationship between urinary 8-iso-prostaglandin F2# , a well
admited marker of oxidative stress, and serum soluble NOX2-derived peptide
(sNOX2-dp) and the severity of liver steatosis in subjects with non-alcoholic fatty
liver. Overall, the study is designed and performed appropriately, including an
ample cohort of patients, although it elicits the drawbacks that the authors
themselves point out in the Discussion section. In summary, the study
demonstrates that the levels of urinary 8-iso-PGF2# were independent predictors
of non-alcoholic fatty liver
and a strong association of urinary 8-iso-PGF2# and of serum sNOX2-dp with
the severity of steatosis at ultrasound was also observed. These findings are
interesting and valid in clinical practice.
Minor essential revisions:
The authors conclude "We demonstrated an increased NOX2-generated
oxidative stress in subjects with non-alcoholic fatty liver. Oxidative stress was
independent from obesity, diabetes and metabolic syndrome and increased with
the severity of liver steatosis at ultrasound." None of the sentences is right.
Serum oxidative stress markers are general markers of the oxidant-antioxidant
balance of the whole body and no measurement of free radicals derived from
NOX2 has been performed. Neither "such oxidative stress" can be said that is
independent of the conditions alluded. Therefore, conclusions should be rewritten
properly.
Although the authors report that subjects underwent routine biochemical
evaluation, they should clearly state how urine and blood collection and
treatment was performed, otherwise experimental conditions may be not
reproducible.
Tables. Parameters should be clearly defined in footnotes in all tables. Revise
commas and dots.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare no competing interest.
Reviewer's report
Title:NOX2-generated oxidative stress is associated with severity of ultrasound
liver steatosis in patients with non-alcoholic fatty liver disease
Version: 3Date:8 October 2013
Reviewer: Begoña Ochoa
Reviewer's report:
Given that few studies have reported increased circulating levels of oxidative
stress markers in patients with non-alcoholic fatty liver and no published study
has been performed with newer markers of systemic oxidative stress, the authors
aim to assess the relationship between urinary 8-iso-prostaglandin F2# , a well
admited marker of oxidative stress, and serum soluble NOX2-derived peptide
(sNOX2-dp) and the severity of liver steatosis in subjects with non-alcoholic fatty
liver. Overall, the study is designed and performed appropriately, including an
ample cohort of patients, although it elicits the drawbacks that the authors
themselves point out in the Discussion section. In summary, the study
demonstrates that the levels of urinary 8-iso-PGF2# were independent predictors
of non-alcoholic fatty liver
and a strong association of urinary 8-iso-PGF2# and of serum sNOX2-dp with
the severity of steatosis at ultrasound was also observed. These findings are
interesting and valid in clinical practice.
Minor essential revisions:
The authors conclude "We demonstrated an increased NOX2-generated
oxidative stress in subjects with non-alcoholic fatty liver. Oxidative stress was
independent from obesity, diabetes and metabolic syndrome and increased with
the severity of liver steatosis at ultrasound." None of the sentences is right.
Serum oxidative stress markers are general markers of the oxidant-antioxidant
balance of the whole body and no measurement of free radicals derived from
NOX2 has been performed. Neither "such oxidative stress" can be said that is
independent of the conditions alluded. Therefore, conclusions should be rewritten
properly.
Although the authors report that subjects underwent routine biochemical
evaluation, they should clearly state how urine and blood collection and
treatment was performed, otherwise experimental conditions may be not
reproducible.
Tables. Parameters should be clearly defined in footnotes in all tables. Revise
commas and dots.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare no competing interest.
Please review this paper.
user
Reviewer's report
Title:NOX2-generated oxidative stress is associated with severity of ultrasound
liver steatosis in patients with non-alcoholic fatty liver disease
Version: 3Date:8 October 2013
Reviewer: Begoña Ochoa
Reviewer's report:
Given that few studies have reported increased circulating levels of oxidative
stress markers in patients with non-alcoholic fatty liver and no published study
has been performed with newer markers of systemic oxidative stress, the authors
aim to assess the relationship between urinary 8-iso-prostaglandin F2# , a well
admited marker of oxidative stress, and serum soluble NOX2-derived peptide
(sNOX2-dp) and the severity of liver steatosis in subjects with non-alcoholic fatty
liver. Overall, the study is designed and performed appropriately, including an
ample cohort of patients, although it elicits the drawbacks that the authors
themselves point out in the Discussion section. In summary, the study
demonstrates that the levels of urinary 8-iso-PGF2# were independent predictors
of non-alcoholic fatty liver
and a strong association of urinary 8-iso-PGF2# and of serum sNOX2-dp with
the severity of steatosis at ultrasound was also observed. These findings are
interesting and valid in clinical practice.
Minor essential revisions:
The authors conclude "We demonstrated an increased NOX2-generated
oxidative stress in subjects with non-alcoholic fatty liver. Oxidative stress was
independent from obesity, diabetes and metabolic syndrome and increased with
the severity of liver steatosis at ultrasound." None of the sentences is right.
Serum oxidative stress markers are general markers of the oxidant-antioxidant
balance of the whole body and no measurement of free radicals derived from
NOX2 has been performed. Neither "such oxidative stress" can be said that is
independent of the conditions alluded. Therefore, conclusions should be rewritten
properly.
Although the authors report that subjects underwent routine biochemical
evaluation, they should clearly state how urine and blood collection and
treatment was performed, otherwise experimental conditions may be not
reproducible.
Tables. Parameters should be clearly defined in footnotes in all tables. Revise
commas and dots.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare no competing interest.
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