Title: ERMAP attenuates DSS-induced colitis  in mice by regulating macrophage and T cell
functions
Version: 1 Date: 17 Dec 2024
Reviewer's report:
This study focused on the role of ERMAP in IBD. In DSS-induced colitis mice, ERMAP protein
increased M2 macrophages, inhibited T cell activation. ERMAP knockdown in macrophages had
opposite effects. ERMAP inhibits NLR pathway. Overall, ERMAP protects colitis by regulating
T and macrophage functions. However, I have some questions regarding this study.
1. Why was the dose of ERMAP-Ig set at 25 µg? What was the basis for this choice? It is
recommended that the authors test two different doses.
2. It is recommended to use Western blot to detect the expression levels of Cleaved-GSDMD,
Cleaved-Caspase1 and Cleaved-IL1# in Fig. 5D.
3. The H&E staining images in Fig. 6E lack pathological scores. It is recommended that the
authors add them.
4. The possible mechanism by which ERMAP regulates the NOD - like receptor signaling
pathway should be discussed.
Title: ERMAP attenuates DSS-induced colitis  in mice by regulating macrophage and T cell
functions
Version: 1 Date: 17 Dec 2024
Reviewer's report:
This study focused on the role of ERMAP in IBD. In DSS-induced colitis mice, ERMAP protein
increased M2 macrophages, inhibited T cell activation. ERMAP knockdown in macrophages had
opposite effects. ERMAP inhibits NLR pathway. Overall, ERMAP protects colitis by regulating
T and macrophage functions. However, I have some questions regarding this study.
1. Why was the dose of ERMAP-Ig set at 25 µg? What was the basis for this choice? It is
recommended that the authors test two different doses.
2. It is recommended to use Western blot to detect the expression levels of Cleaved-GSDMD,
Cleaved-Caspase1 and Cleaved-IL1# in Fig. 5D.
3. The H&E staining images in Fig. 6E lack pathological scores. It is recommended that the
authors add them.
4. The possible mechanism by which ERMAP regulates the NOD - like receptor signaling
pathway should be discussed.
Please review this paper.
user
Title: ERMAP attenuates DSS-induced colitis  in mice by regulating macrophage and T cell
functions
Version: 1 Date: 17 Dec 2024
Reviewer's report:
This study focused on the role of ERMAP in IBD. In DSS-induced colitis mice, ERMAP protein
increased M2 macrophages, inhibited T cell activation. ERMAP knockdown in macrophages had
opposite effects. ERMAP inhibits NLR pathway. Overall, ERMAP protects colitis by regulating
T and macrophage functions. However, I have some questions regarding this study.
1. Why was the dose of ERMAP-Ig set at 25 µg? What was the basis for this choice? It is
recommended that the authors test two different doses.
2. It is recommended to use Western blot to detect the expression levels of Cleaved-GSDMD,
Cleaved-Caspase1 and Cleaved-IL1# in Fig. 5D.
3. The H&E staining images in Fig. 6E lack pathological scores. It is recommended that the
authors add them.
4. The possible mechanism by which ERMAP regulates the NOD - like receptor signaling
pathway should be discussed.
assistant