Reviewer ’s report  
Title: A New Method to Induce Nonalcoholic Steatohepatitis (NASH) in Mice  
Version: 1 Date: 20 Oct 2018  
Reviewer: Manuella Lanzetti  
Reviewer's report:  
Please include all comments for the authors in this box rather than uploading your report as an 
attachment. Please only upload as attachments annotated versions of manuscripts, graphs, 
supporting materials or other aspects of your report which cannot be in cluded in a text format.  
Please overwrite this text when adding your comments to the authors.  
The study "A New Method to Induce Nonalcoholic Steatohepatitis (NASH) in Mice" has a 
descriptive approach about different diets to induce NAFLD. It was well cond ucted and written, 
regarding some crucial tools to conduct the conclusion. The authors, also investigated the action 
of cigarette smoke in every experimental models included in this study, as a co -factor for hepatic 
damage. None important intervention is n eeded to publication by this journal. However, any 
mechanism pathway was investigated to better elucidate the obtained data, specially in concern 
about the relation of cigarette smoke and the increased risk to hepatic lesions in some diet tested.   
About t he cigarette smoke model used in this study, I recommend a better explanation about the 
employed dose of 4 cigarettes/day, once it can be consider a low smoking load to develop 
chronic diseases.  
 
Some minor corrections can be done.  
On pdf page 11, line 1 1: use "serum level" instead of "Serum level".  
On page 12, line 17: use "inflammation"instead of "Inflammation". Add a dot after "(central 
vein)".  
Please, consider review the term "prounced"on page 16, line 15.  
On figure legends, please mention the congo red stain on figure 2 legend and also include the 
magnification of the images.    
Are the methods appropriate and well described?  
If not, please specify what is requi red in your comments to the authors.  
 
Yes 
 
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
 
Yes 
 
Are the conclusions drawn adequately supported by the data shown?  
If not, plea se explain in your comments to the authors.  
 
Yes 
 
Are you able to assess any statistics in the manuscript or would you recommend an 
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects require furth er 
assessment in your comments to the editors.  
 
I am able to assess the statistics  
 
Quality of written English  
Please indicate the quality of language in the manuscript:  
 
Acceptable  
 
Declaration of competing interests  
Please complete a declaration of competi ng interests, considering the following questions:  
 
1. Have you in the past five years received reimbursements, fees, funding, or salary from an 
organisation that may in any way gain or lose financially from the publication of this 
manuscript, either now or i n the future?  
 
2. Do you hold any stocks or shares in an organisation that may in any way gain or lose 
financially from the publication of this manuscript, either now or in the future?  
 
3. Do you hold or are you currently applying for any patents relating to the  content of the 
manuscript?  
 
4. Have you received reimbursements, fees, funding, or salary from an organization that 
holds or has applied for patents relating to the content of the manuscript?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have  any non -financial competing interests in relation to this paper?  
 
If you can answer no to all of the above, write 'I declare that I have no competing interests' 
below. If your reply is yes to any, please give details below.  
 
I declare that I have no competing interest.  
 
I agree to the open peer  review policy of the journal. I understand that my name will be included 
on my report to the authors and, if the manuscript is accepted for publication, my named report 
including any attachments I upload will be posted on the website along with the author s' 
responses. I agree for my report to be made available under an Open Access Creative Commons 
CC-BY license ( http://creativecommons.org/licenses/by/4.0/ ).  I understand that any comments 
which I do not wish to be included in my named report can be included as confidential comments 
to the editors, which will not be published.  
 
I agree to the open peer review policy of the journal  
Reviewer ’s report  
Title: A New Method to Induce Nonalcoholic Steatohepatitis (NASH) in Mice  
Version: 1 Date: 20 Oct 2018  
Reviewer: Manuella Lanzetti  
Reviewer's report:  
Please include all comments for the authors in this box rather than uploading your report as an 
attachment. Please only upload as attachments annotated versions of manuscripts, graphs, 
supporting materials or other aspects of your report which cannot be in cluded in a text format.  
Please overwrite this text when adding your comments to the authors.  
The study "A New Method to Induce Nonalcoholic Steatohepatitis (NASH) in Mice" has a 
descriptive approach about different diets to induce NAFLD. It was well cond ucted and written, 
regarding some crucial tools to conduct the conclusion. The authors, also investigated the action 
of cigarette smoke in every experimental models included in this study, as a co -factor for hepatic 
damage. None important intervention is n eeded to publication by this journal. However, any 
mechanism pathway was investigated to better elucidate the obtained data, specially in concern 
about the relation of cigarette smoke and the increased risk to hepatic lesions in some diet tested.   
About t he cigarette smoke model used in this study, I recommend a better explanation about the 
employed dose of 4 cigarettes/day, once it can be consider a low smoking load to develop 
chronic diseases.  
 
Some minor corrections can be done.  
On pdf page 11, line 1 1: use "serum level" instead of "Serum level".  
On page 12, line 17: use "inflammation"instead of "Inflammation". Add a dot after "(central 
vein)".  
Please, consider review the term "prounced"on page 16, line 15.  
On figure legends, please mention the congo red stain on figure 2 legend and also include the 
magnification of the images.    
Are the methods appropriate and well described?  
If not, please specify what is requi red in your comments to the authors.  
 
Yes 
 
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
 
Yes 
 
Are the conclusions drawn adequately supported by the data shown?  
If not, plea se explain in your comments to the authors.  
 
Yes 
 
Are you able to assess any statistics in the manuscript or would you recommend an 
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects require furth er 
assessment in your comments to the editors.  
 
I am able to assess the statistics  
 
Quality of written English  
Please indicate the quality of language in the manuscript:  
 
Acceptable  
 
Declaration of competing interests  
Please complete a declaration of competi ng interests, considering the following questions:  
 
1. Have you in the past five years received reimbursements, fees, funding, or salary from an 
organisation that may in any way gain or lose financially from the publication of this 
manuscript, either now or i n the future?  
 
2. Do you hold any stocks or shares in an organisation that may in any way gain or lose 
financially from the publication of this manuscript, either now or in the future?  
 
3. Do you hold or are you currently applying for any patents relating to the  content of the 
manuscript?  
 
4. Have you received reimbursements, fees, funding, or salary from an organization that 
holds or has applied for patents relating to the content of the manuscript?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have  any non -financial competing interests in relation to this paper?  
 
If you can answer no to all of the above, write 'I declare that I have no competing interests' 
below. If your reply is yes to any, please give details below.  
 
I declare that I have no competing interest.  
 
I agree to the open peer  review policy of the journal. I understand that my name will be included 
on my report to the authors and, if the manuscript is accepted for publication, my named report 
including any attachments I upload will be posted on the website along with the author s' 
responses. I agree for my report to be made available under an Open Access Creative Commons 
CC-BY license ( http://creativecommons.org/licenses/by/4.0/ ).  I understand that any comments 
which I do not wish to be included in my named report can be included as confidential comments 
to the editors, which will not be published.  
 
I agree to the open peer review policy of the journal  
Please review this paper.
user
Reviewer ’s report  
Title: A New Method to Induce Nonalcoholic Steatohepatitis (NASH) in Mice  
Version: 1 Date: 20 Oct 2018  
Reviewer: Manuella Lanzetti  
Reviewer's report:  
Please include all comments for the authors in this box rather than uploading your report as an 
attachment. Please only upload as attachments annotated versions of manuscripts, graphs, 
supporting materials or other aspects of your report which cannot be in cluded in a text format.  
Please overwrite this text when adding your comments to the authors.  
The study "A New Method to Induce Nonalcoholic Steatohepatitis (NASH) in Mice" has a 
descriptive approach about different diets to induce NAFLD. It was well cond ucted and written, 
regarding some crucial tools to conduct the conclusion. The authors, also investigated the action 
of cigarette smoke in every experimental models included in this study, as a co -factor for hepatic 
damage. None important intervention is n eeded to publication by this journal. However, any 
mechanism pathway was investigated to better elucidate the obtained data, specially in concern 
about the relation of cigarette smoke and the increased risk to hepatic lesions in some diet tested.   
About t he cigarette smoke model used in this study, I recommend a better explanation about the 
employed dose of 4 cigarettes/day, once it can be consider a low smoking load to develop 
chronic diseases.  
 
Some minor corrections can be done.  
On pdf page 11, line 1 1: use "serum level" instead of "Serum level".  
On page 12, line 17: use "inflammation"instead of "Inflammation". Add a dot after "(central 
vein)".  
Please, consider review the term "prounced"on page 16, line 15.  
On figure legends, please mention the congo red stain on figure 2 legend and also include the 
magnification of the images.    
Are the methods appropriate and well described?  
If not, please specify what is requi red in your comments to the authors.  
 
Yes 
 
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
 
Yes 
 
Are the conclusions drawn adequately supported by the data shown?  
If not, plea se explain in your comments to the authors.  
 
Yes 
 
Are you able to assess any statistics in the manuscript or would you recommend an 
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects require furth er 
assessment in your comments to the editors.  
 
I am able to assess the statistics  
 
Quality of written English  
Please indicate the quality of language in the manuscript:  
 
Acceptable  
 
Declaration of competing interests  
Please complete a declaration of competi ng interests, considering the following questions:  
 
1. Have you in the past five years received reimbursements, fees, funding, or salary from an 
organisation that may in any way gain or lose financially from the publication of this 
manuscript, either now or i n the future?  
 
2. Do you hold any stocks or shares in an organisation that may in any way gain or lose 
financially from the publication of this manuscript, either now or in the future?  
 
3. Do you hold or are you currently applying for any patents relating to the  content of the 
manuscript?  
 
4. Have you received reimbursements, fees, funding, or salary from an organization that 
holds or has applied for patents relating to the content of the manuscript?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have  any non -financial competing interests in relation to this paper?  
 
If you can answer no to all of the above, write 'I declare that I have no competing interests' 
below. If your reply is yes to any, please give details below.  
 
I declare that I have no competing interest.  
 
I agree to the open peer  review policy of the journal. I understand that my name will be included 
on my report to the authors and, if the manuscript is accepted for publication, my named report 
including any attachments I upload will be posted on the website along with the author s' 
responses. I agree for my report to be made available under an Open Access Creative Commons 
CC-BY license ( http://creativecommons.org/licenses/by/4.0/ ).  I understand that any comments 
which I do not wish to be included in my named report can be included as confidential comments 
to the editors, which will not be published.  
 
I agree to the open peer review policy of the journal  
assistant