Title: Correlation and Diagnostic Significance of CD4 T Cell Subsets and NLRP3
Inflammasome in Ulcerative Colitis: The Role of the NLRP3/T-bet/GATA3 Axis
Version: 1 Date: 18 Nov 2024
Reviewer's report:
This scientific paper provides information regarding the relation between CD4 T cell subsets
and the activation of NLRP3 inflammasome in IBD patients, more specifically in UC patients,
and its therapeutic potential as biomarkers of the disease diagnosis, severity and response to
treatment. It was well written and adequately executed, and the assumption was supported by
analyzing the serum and colonic expression of CD4 T cell cytokines and proteins of the NLRP3/
T-bet/GATA3 Axis, which are known to be involved in the etiopathology of UC, which showed
distinct levels between the controls and the UC-LM and UC-MH patients, and in addition a
correlation between some of those factors was proven.
Having said that, however, there is still room for improvement.
Specifically, in the Results section, throughout all the paragraphs, the P values are mistakenly
written as “P>0.05” etc., instead of "P<0.05” etc. as shown in the Figure legends and is usually
presented. It should be corrected. In addition, I believe that it would benefit the text in the Results
section if it included the Medians as well as the IQRs of each statistically significant result,
in order to give more detailed results and provide a better understanding of their significance,
instead of just mentioning the P values.
Last but not least, some minor points include:
1) In the line 95 of the Introduction, where it is presented as “NLRP3 inflammasome has
identified as a pivotal element” while it should be “NLRP3 inflammasome was identified as a
pivotal element”.
2) In the line 319 of the Discussion, where it is presented as “differences among the groupsa”
while it should be "differences among the groups”.
3) In the line 324 of the Discussion, where it is presented as “increased IL-2 exacerbats
inflammation” while it should be “increased IL-2 exacerbates inflammation”.
Title: Correlation and Diagnostic Significance of CD4 T Cell Subsets and NLRP3
Inflammasome in Ulcerative Colitis: The Role of the NLRP3/T-bet/GATA3 Axis
Version: 1 Date: 18 Nov 2024
Reviewer's report:
This scientific paper provides information regarding the relation between CD4 T cell subsets
and the activation of NLRP3 inflammasome in IBD patients, more specifically in UC patients,
and its therapeutic potential as biomarkers of the disease diagnosis, severity and response to
treatment. It was well written and adequately executed, and the assumption was supported by
analyzing the serum and colonic expression of CD4 T cell cytokines and proteins of the NLRP3/
T-bet/GATA3 Axis, which are known to be involved in the etiopathology of UC, which showed
distinct levels between the controls and the UC-LM and UC-MH patients, and in addition a
correlation between some of those factors was proven.
Having said that, however, there is still room for improvement.
Specifically, in the Results section, throughout all the paragraphs, the P values are mistakenly
written as “P>0.05” etc., instead of "P<0.05” etc. as shown in the Figure legends and is usually
presented. It should be corrected. In addition, I believe that it would benefit the text in the Results
section if it included the Medians as well as the IQRs of each statistically significant result,
in order to give more detailed results and provide a better understanding of their significance,
instead of just mentioning the P values.
Last but not least, some minor points include:
1) In the line 95 of the Introduction, where it is presented as “NLRP3 inflammasome has
identified as a pivotal element” while it should be “NLRP3 inflammasome was identified as a
pivotal element”.
2) In the line 319 of the Discussion, where it is presented as “differences among the groupsa”
while it should be "differences among the groups”.
3) In the line 324 of the Discussion, where it is presented as “increased IL-2 exacerbats
inflammation” while it should be “increased IL-2 exacerbates inflammation”.
Please review this paper.
user
Title: Correlation and Diagnostic Significance of CD4 T Cell Subsets and NLRP3
Inflammasome in Ulcerative Colitis: The Role of the NLRP3/T-bet/GATA3 Axis
Version: 1 Date: 18 Nov 2024
Reviewer's report:
This scientific paper provides information regarding the relation between CD4 T cell subsets
and the activation of NLRP3 inflammasome in IBD patients, more specifically in UC patients,
and its therapeutic potential as biomarkers of the disease diagnosis, severity and response to
treatment. It was well written and adequately executed, and the assumption was supported by
analyzing the serum and colonic expression of CD4 T cell cytokines and proteins of the NLRP3/
T-bet/GATA3 Axis, which are known to be involved in the etiopathology of UC, which showed
distinct levels between the controls and the UC-LM and UC-MH patients, and in addition a
correlation between some of those factors was proven.
Having said that, however, there is still room for improvement.
Specifically, in the Results section, throughout all the paragraphs, the P values are mistakenly
written as “P>0.05” etc., instead of "P<0.05” etc. as shown in the Figure legends and is usually
presented. It should be corrected. In addition, I believe that it would benefit the text in the Results
section if it included the Medians as well as the IQRs of each statistically significant result,
in order to give more detailed results and provide a better understanding of their significance,
instead of just mentioning the P values.
Last but not least, some minor points include:
1) In the line 95 of the Introduction, where it is presented as “NLRP3 inflammasome has
identified as a pivotal element” while it should be “NLRP3 inflammasome was identified as a
pivotal element”.
2) In the line 319 of the Discussion, where it is presented as “differences among the groupsa”
while it should be "differences among the groups”.
3) In the line 324 of the Discussion, where it is presented as “increased IL-2 exacerbats
inflammation” while it should be “increased IL-2 exacerbates inflammation”.
assistant