Reviewer's report
Title:Expression of energy metabolism related genes in the gastric tissue of
obese individuals with non-alcoholic fatty liver disease
Version: 1Date:16 July 2013
Reviewer: Munechika Enjoji
Reviewer's report:
Authors presented the expression profiles of energy metabolism associated
genes in gastric tissue of obese NAFLD patients and assessed the association of
the expression levels with hepatic steatosis, inflammation, fibrosis, and NASH.
This study includes novel findings which may be helpful for investigation of
NAFLD. Then, this report has potential for publication, I think. But there are some
incoherent expressions in the text. Major Compulsory Revisions are needed.
Comments
1. [Abstract: 1st line] Authors wrote “this energy balance…..”. What is the
meaning of “this”?
2. [Methods] What extent of gastric wall was included in your samples for PCR,
mucosa only, with submucosa, or entire gastric wall? Which kind of cells is
important for this study?
3. [Abstract & Discussion] Authors wrote “accumulation of the fat in the liver
parenchyma is accompanied by downregulation of genes encoding for CPE and
IL1B in the gastric mucosa of same patient”. But the data of expression levels of
these genes were not shown in different stage of fatty liver in same patient.
4. Do you have any data of patients without obesity, which may be a better
control?
5. [Methods] Please show us the definition of morbidly obese NAFLD patients.
How to select the enrolled patients?
6. [Introduction] More detailed explanation of your previous manuscript
(reference No 3) should be presented.
7. [Table 1] Patient number of advanced inflammation is 13. But the number of
steatosis with advanced inflammation (n=23) and that of NASH with advanced
inflammation (n=18) are larger than 13. In addition, the number of NASH with
advanced inflammation (n=18) are larger than that of NASH (n=15).
8. [Figures] I do not get the meaning of Figure 2. Are these figures required?
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Reviewer's report
Title:Expression of energy metabolism related genes in the gastric tissue of
obese individuals with non-alcoholic fatty liver disease
Version: 1Date:16 July 2013
Reviewer: Munechika Enjoji
Reviewer's report:
Authors presented the expression profiles of energy metabolism associated
genes in gastric tissue of obese NAFLD patients and assessed the association of
the expression levels with hepatic steatosis, inflammation, fibrosis, and NASH.
This study includes novel findings which may be helpful for investigation of
NAFLD. Then, this report has potential for publication, I think. But there are some
incoherent expressions in the text. Major Compulsory Revisions are needed.
Comments
1. [Abstract: 1st line] Authors wrote “this energy balance…..”. What is the
meaning of “this”?
2. [Methods] What extent of gastric wall was included in your samples for PCR,
mucosa only, with submucosa, or entire gastric wall? Which kind of cells is
important for this study?
3. [Abstract & Discussion] Authors wrote “accumulation of the fat in the liver
parenchyma is accompanied by downregulation of genes encoding for CPE and
IL1B in the gastric mucosa of same patient”. But the data of expression levels of
these genes were not shown in different stage of fatty liver in same patient.
4. Do you have any data of patients without obesity, which may be a better
control?
5. [Methods] Please show us the definition of morbidly obese NAFLD patients.
How to select the enrolled patients?
6. [Introduction] More detailed explanation of your previous manuscript
(reference No 3) should be presented.
7. [Table 1] Patient number of advanced inflammation is 13. But the number of
steatosis with advanced inflammation (n=23) and that of NASH with advanced
inflammation (n=18) are larger than 13. In addition, the number of NASH with
advanced inflammation (n=18) are larger than that of NASH (n=15).
8. [Figures] I do not get the meaning of Figure 2. Are these figures required?
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Please review this paper.
user
Reviewer's report
Title:Expression of energy metabolism related genes in the gastric tissue of
obese individuals with non-alcoholic fatty liver disease
Version: 1Date:16 July 2013
Reviewer: Munechika Enjoji
Reviewer's report:
Authors presented the expression profiles of energy metabolism associated
genes in gastric tissue of obese NAFLD patients and assessed the association of
the expression levels with hepatic steatosis, inflammation, fibrosis, and NASH.
This study includes novel findings which may be helpful for investigation of
NAFLD. Then, this report has potential for publication, I think. But there are some
incoherent expressions in the text. Major Compulsory Revisions are needed.
Comments
1. [Abstract: 1st line] Authors wrote “this energy balance…..”. What is the
meaning of “this”?
2. [Methods] What extent of gastric wall was included in your samples for PCR,
mucosa only, with submucosa, or entire gastric wall? Which kind of cells is
important for this study?
3. [Abstract & Discussion] Authors wrote “accumulation of the fat in the liver
parenchyma is accompanied by downregulation of genes encoding for CPE and
IL1B in the gastric mucosa of same patient”. But the data of expression levels of
these genes were not shown in different stage of fatty liver in same patient.
4. Do you have any data of patients without obesity, which may be a better
control?
5. [Methods] Please show us the definition of morbidly obese NAFLD patients.
How to select the enrolled patients?
6. [Introduction] More detailed explanation of your previous manuscript
(reference No 3) should be presented.
7. [Table 1] Patient number of advanced inflammation is 13. But the number of
steatosis with advanced inflammation (n=23) and that of NASH with advanced
inflammation (n=18) are larger than 13. In addition, the number of NASH with
advanced inflammation (n=18) are larger than that of NASH (n=15).
8. [Figures] I do not get the meaning of Figure 2. Are these figures required?
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
assistant