Reviewer's report
Title:Novel application of low pH-dependent fluorescent dyes to examine colitis
Version: 1Date:6 October 2009
Reviewer: Carmen Waengler
Reviewer's report:
Minor essential revisions to be made:
1.
The authors compare their fluorescent dye NMD-PZ to others, namely
LysoSensor Green DND-153 and DND-189 but do not mention their suitability for
the detection of colitis in the introduction. To complete the background in the
introduction, these dyes should be mentioned as the authors themselves
investigated them with regard to low pH applications in “Ishiguro K, Ando T, Goto
H: Novel application of 4-nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole to visualize
lysosomes in live cells. Biotechniques 2008, 45 (4):465, 467-468”.
2.
In Figure 7 are shown the results from a mutagenicity test of NBD-PZ. It can be
seen that a concentration of up to 2.5 µg/ml NBD-PZ results in no mutation.
However, a concentration of 10 µg/ml exhibits a low but apparent mutagenicity
and the authors themselves state that a “careful monitoring should be performed
at a clinical trial of NBD-PZ application to avoid any harmful events” in their
discussion. Therefore, the authors should state in their conclusion that not only
the clinical validity of the results remains to be determined but also the
harmlessness of the compound.
3.
“We applied NBD-PZ to the mucosa of TNBS-induced colitis and found that there
was a
disappearance of the ring-shaped fluorescent stains that were observed in the
normal
colon (Fig. 2A-D).”
Change to: … observed in the normal colon (Fig. 1A-D).
4.
Please make clear the table legends that every circle stands for one experiment
performed.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Reviewer's report
Title:Novel application of low pH-dependent fluorescent dyes to examine colitis
Version: 1Date:6 October 2009
Reviewer: Carmen Waengler
Reviewer's report:
Minor essential revisions to be made:
1.
The authors compare their fluorescent dye NMD-PZ to others, namely
LysoSensor Green DND-153 and DND-189 but do not mention their suitability for
the detection of colitis in the introduction. To complete the background in the
introduction, these dyes should be mentioned as the authors themselves
investigated them with regard to low pH applications in “Ishiguro K, Ando T, Goto
H: Novel application of 4-nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole to visualize
lysosomes in live cells. Biotechniques 2008, 45 (4):465, 467-468”.
2.
In Figure 7 are shown the results from a mutagenicity test of NBD-PZ. It can be
seen that a concentration of up to 2.5 µg/ml NBD-PZ results in no mutation.
However, a concentration of 10 µg/ml exhibits a low but apparent mutagenicity
and the authors themselves state that a “careful monitoring should be performed
at a clinical trial of NBD-PZ application to avoid any harmful events” in their
discussion. Therefore, the authors should state in their conclusion that not only
the clinical validity of the results remains to be determined but also the
harmlessness of the compound.
3.
“We applied NBD-PZ to the mucosa of TNBS-induced colitis and found that there
was a
disappearance of the ring-shaped fluorescent stains that were observed in the
normal
colon (Fig. 2A-D).”
Change to: … observed in the normal colon (Fig. 1A-D).
4.
Please make clear the table legends that every circle stands for one experiment
performed.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Please review this paper.
user
Reviewer's report
Title:Novel application of low pH-dependent fluorescent dyes to examine colitis
Version: 1Date:6 October 2009
Reviewer: Carmen Waengler
Reviewer's report:
Minor essential revisions to be made:
1.
The authors compare their fluorescent dye NMD-PZ to others, namely
LysoSensor Green DND-153 and DND-189 but do not mention their suitability for
the detection of colitis in the introduction. To complete the background in the
introduction, these dyes should be mentioned as the authors themselves
investigated them with regard to low pH applications in “Ishiguro K, Ando T, Goto
H: Novel application of 4-nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole to visualize
lysosomes in live cells. Biotechniques 2008, 45 (4):465, 467-468”.
2.
In Figure 7 are shown the results from a mutagenicity test of NBD-PZ. It can be
seen that a concentration of up to 2.5 µg/ml NBD-PZ results in no mutation.
However, a concentration of 10 µg/ml exhibits a low but apparent mutagenicity
and the authors themselves state that a “careful monitoring should be performed
at a clinical trial of NBD-PZ application to avoid any harmful events” in their
discussion. Therefore, the authors should state in their conclusion that not only
the clinical validity of the results remains to be determined but also the
harmlessness of the compound.
3.
“We applied NBD-PZ to the mucosa of TNBS-induced colitis and found that there
was a
disappearance of the ring-shaped fluorescent stains that were observed in the
normal
colon (Fig. 2A-D).”
Change to: … observed in the normal colon (Fig. 1A-D).
4.
Please make clear the table legends that every circle stands for one experiment
performed.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
assistant