Title: Effect of probiotics combined with immune checkpoint suppressors and chemotherapeutic
agents on digestive system function, intestinal immunity and prognosis in patients with
metastatic colorectal carcinoma: a quasi-experimental study
Version: 0 Date: 20 Dec 2024
Reviewer's report:
1.Whether this manuscript is a retrospective or prospective study, we failed to find out in the
manuscript. For a retrospective study, how many cases were collected, how many cases met the
inclusion criteria, and how many cases were excluded, and 96 cases remained. They were then
divided into control and study groups as treated, and this process is described in a Fig. If it is
a prospective study,  the selected size of the sample , the method of grouping, etc.should be
describe . Please explain it.
2.In the Methods section, please describe the time of each treatment cycle.
3.The names of the two groups are inconsistent in the text: study group or research group or R
group; control group or C group, please modified.
4.In the chart, the marker for statistical results are inconsistent, A should be changed to a
5.In the observation index, the observation point of each index should be indicated as 4 treatment
cycles, which are not specified in the stool culture and quality of life.
6.In the discussion, this sentence:‘Our findings indicated that the levels of D-LA and DAO were
elevated, the and the increase in the study group was smaller after treatment’ should be placed
in the upper paragraph.
7.In the graphical representation of survival groups, six weeks, 12 weeks and two years were
inconsistent. Please modify.
8.This sentence ‘Immune checkpoint suppressor-based and chemotherapy in combination with
probiotic administration did not influence the prognosis of the patients’Inconsistent with the
result, please modify it
9‘Note: the control group before and after treatment, aP < 0.05; Comparison of the study
group before and after treatment, bP < 0.05.’may be#Compare with Before treament, a:P <
 0.05;Compare with control group,b:P < 0.05.Please checkout and revise it.
Title: Effect of probiotics combined with immune checkpoint suppressors and chemotherapeutic
agents on digestive system function, intestinal immunity and prognosis in patients with
metastatic colorectal carcinoma: a quasi-experimental study
Version: 0 Date: 20 Dec 2024
Reviewer's report:
1.Whether this manuscript is a retrospective or prospective study, we failed to find out in the
manuscript. For a retrospective study, how many cases were collected, how many cases met the
inclusion criteria, and how many cases were excluded, and 96 cases remained. They were then
divided into control and study groups as treated, and this process is described in a Fig. If it is
a prospective study,  the selected size of the sample , the method of grouping, etc.should be
describe . Please explain it.
2.In the Methods section, please describe the time of each treatment cycle.
3.The names of the two groups are inconsistent in the text: study group or research group or R
group; control group or C group, please modified.
4.In the chart, the marker for statistical results are inconsistent, A should be changed to a
5.In the observation index, the observation point of each index should be indicated as 4 treatment
cycles, which are not specified in the stool culture and quality of life.
6.In the discussion, this sentence:‘Our findings indicated that the levels of D-LA and DAO were
elevated, the and the increase in the study group was smaller after treatment’ should be placed
in the upper paragraph.
7.In the graphical representation of survival groups, six weeks, 12 weeks and two years were
inconsistent. Please modify.
8.This sentence ‘Immune checkpoint suppressor-based and chemotherapy in combination with
probiotic administration did not influence the prognosis of the patients’Inconsistent with the
result, please modify it
9‘Note: the control group before and after treatment, aP < 0.05; Comparison of the study
group before and after treatment, bP < 0.05.’may be#Compare with Before treament, a:P <
 0.05;Compare with control group,b:P < 0.05.Please checkout and revise it.
Please review this paper.
user
Title: Effect of probiotics combined with immune checkpoint suppressors and chemotherapeutic
agents on digestive system function, intestinal immunity and prognosis in patients with
metastatic colorectal carcinoma: a quasi-experimental study
Version: 0 Date: 20 Dec 2024
Reviewer's report:
1.Whether this manuscript is a retrospective or prospective study, we failed to find out in the
manuscript. For a retrospective study, how many cases were collected, how many cases met the
inclusion criteria, and how many cases were excluded, and 96 cases remained. They were then
divided into control and study groups as treated, and this process is described in a Fig. If it is
a prospective study,  the selected size of the sample , the method of grouping, etc.should be
describe . Please explain it.
2.In the Methods section, please describe the time of each treatment cycle.
3.The names of the two groups are inconsistent in the text: study group or research group or R
group; control group or C group, please modified.
4.In the chart, the marker for statistical results are inconsistent, A should be changed to a
5.In the observation index, the observation point of each index should be indicated as 4 treatment
cycles, which are not specified in the stool culture and quality of life.
6.In the discussion, this sentence:‘Our findings indicated that the levels of D-LA and DAO were
elevated, the and the increase in the study group was smaller after treatment’ should be placed
in the upper paragraph.
7.In the graphical representation of survival groups, six weeks, 12 weeks and two years were
inconsistent. Please modify.
8.This sentence ‘Immune checkpoint suppressor-based and chemotherapy in combination with
probiotic administration did not influence the prognosis of the patients’Inconsistent with the
result, please modify it
9‘Note: the control group before and after treatment, aP < 0.05; Comparison of the study
group before and after treatment, bP < 0.05.’may be#Compare with Before treament, a:P <
 0.05;Compare with control group,b:P < 0.05.Please checkout and revise it.
assistant