Reviewer's report
Title:Glucagon-like peptides 1 and 2 and vasoactive intestinal peptide are
neuroprotective on cultured and mast cell co-cultured rat myenteric neurons
Version: 1Date:20 December 2011
Reviewer: Tanya Little
Reviewer's report:
This is a novel study. The authors aimed to determine whether the gut peptides
GLP-1, GLP-1 and VIP had neuroprotective effects on rat cultured myenteric
neurons. The series of experiments performed using previously established
techniques provide a solid answer to the question posed.
The authors have demonstrated protective effects of the gut peptides GLP-1 and
GLP-2 and VIP, on cultured myenteric neurons. These protective effects were
also demonstrated in myenteric neurons that were co-cultured with mast cells.
These observations were significantly strengthened by the demonstration that
these effects were reversed in the presence of specific antagonists of the GLP-1
and VIP receptors.
The discussion and conclusions are well balanced and supported by the data.
The authors clearly acknowledge previous work in the area, and discuss in
context with the current results. Limitations of the work are discussed.
Minor Essential Revisions
-Abstract, rephrase sentence referring to common super family of peptides.
-Methods, page 10, paragraph 2. The statement ‘followed by exposure of GLP-1,
GLP-2, or VIP” should read “followed by exposure to GLP-1, GLP-2, or VIP.”
Methods - comment on how the doses of GLP-1 GLP-2 and VIP used would
relate to concentrations found in vivo.
Results: page 10, please provide units after values for Emax.
-Figure 1 legend: Final line, the legend refers to G, instead of F.
-Please check the tense used throughout.
-Please consistently refer to hypbVIP as a VIP-receptor antagonist rather than a
VIP antagonist.
Level of interest: An article of outstanding merit and interest in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:Glucagon-like peptides 1 and 2 and vasoactive intestinal peptide are
neuroprotective on cultured and mast cell co-cultured rat myenteric neurons
Version: 1Date:20 December 2011
Reviewer: Tanya Little
Reviewer's report:
This is a novel study. The authors aimed to determine whether the gut peptides
GLP-1, GLP-1 and VIP had neuroprotective effects on rat cultured myenteric
neurons. The series of experiments performed using previously established
techniques provide a solid answer to the question posed.
The authors have demonstrated protective effects of the gut peptides GLP-1 and
GLP-2 and VIP, on cultured myenteric neurons. These protective effects were
also demonstrated in myenteric neurons that were co-cultured with mast cells.
These observations were significantly strengthened by the demonstration that
these effects were reversed in the presence of specific antagonists of the GLP-1
and VIP receptors.
The discussion and conclusions are well balanced and supported by the data.
The authors clearly acknowledge previous work in the area, and discuss in
context with the current results. Limitations of the work are discussed.
Minor Essential Revisions
-Abstract, rephrase sentence referring to common super family of peptides.
-Methods, page 10, paragraph 2. The statement ‘followed by exposure of GLP-1,
GLP-2, or VIP” should read “followed by exposure to GLP-1, GLP-2, or VIP.”
Methods - comment on how the doses of GLP-1 GLP-2 and VIP used would
relate to concentrations found in vivo.
Results: page 10, please provide units after values for Emax.
-Figure 1 legend: Final line, the legend refers to G, instead of F.
-Please check the tense used throughout.
-Please consistently refer to hypbVIP as a VIP-receptor antagonist rather than a
VIP antagonist.
Level of interest: An article of outstanding merit and interest in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:Glucagon-like peptides 1 and 2 and vasoactive intestinal peptide are
neuroprotective on cultured and mast cell co-cultured rat myenteric neurons
Version: 1Date:20 December 2011
Reviewer: Tanya Little
Reviewer's report:
This is a novel study. The authors aimed to determine whether the gut peptides
GLP-1, GLP-1 and VIP had neuroprotective effects on rat cultured myenteric
neurons. The series of experiments performed using previously established
techniques provide a solid answer to the question posed.
The authors have demonstrated protective effects of the gut peptides GLP-1 and
GLP-2 and VIP, on cultured myenteric neurons. These protective effects were
also demonstrated in myenteric neurons that were co-cultured with mast cells.
These observations were significantly strengthened by the demonstration that
these effects were reversed in the presence of specific antagonists of the GLP-1
and VIP receptors.
The discussion and conclusions are well balanced and supported by the data.
The authors clearly acknowledge previous work in the area, and discuss in
context with the current results. Limitations of the work are discussed.
Minor Essential Revisions
-Abstract, rephrase sentence referring to common super family of peptides.
-Methods, page 10, paragraph 2. The statement ‘followed by exposure of GLP-1,
GLP-2, or VIP” should read “followed by exposure to GLP-1, GLP-2, or VIP.”
Methods - comment on how the doses of GLP-1 GLP-2 and VIP used would
relate to concentrations found in vivo.
Results: page 10, please provide units after values for Emax.
-Figure 1 legend: Final line, the legend refers to G, instead of F.
-Please check the tense used throughout.
-Please consistently refer to hypbVIP as a VIP-receptor antagonist rather than a
VIP antagonist.
Level of interest: An article of outstanding merit and interest in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
assistant