Title: Identification and immunological characterization of cuproptosis-related molecular
clusters in Ulcerative Colitis
Version: 2 Date: 21 Feb 2023
Reviewer's report:
The paper named “Identification and immunological characterization of cuproptosis-related
molecular clusters in Ulcerative Colitis” conducted by Pu et al. has been evaluated. I think this
study has certain innovation and clinical significance. In this study, the author comprehensively
explored the cuproptosis-related genes in ulcerative colitis. However, some obvious limitations
impair the overall quality. Before it is suitable for publication, I suggest the author make
substantive modifications.
1. However, I noticed that some of the figures have a low resolution which makes them hard
to read. I would suggest that the authors increase the resolution of these figures, which would
make them easier to read and understand. This would also make it easier for readers to fully
understand the results and make use of it. I believe that increasing the resolution of these figures
would greatly improve the manuscript.
2. Some previous studies have used similar methods or statistical methods. Although it will
lose some innovation, I think it will benefit the reader if the authors could include the
following references, which would provide readers with a more complete understanding of the
whole study (PMID: 33850225, PMID: 34099740, PMID: 35277527, PMID: 33223511, PMID:
34358932 ...).
3. Detailed description of all method sections is required.
4. State the * and statistic value in the method section or otherwise mention in every figure
legend. e.g., *p>0.05.
5. Check the reference style and abbreviation of journals.
6. Some typos are there; rectify those (Minor).
7. the relationship between immune cell infiltration and specifc pathways should be well
discussed in the relevant section.
8. The result of figure 9D seems to be over-fitting. Can the author take measures or explain how
he avoids the risk of over-fitting?
Title: Identification and immunological characterization of cuproptosis-related molecular
clusters in Ulcerative Colitis
Version: 2 Date: 21 Feb 2023
Reviewer's report:
The paper named “Identification and immunological characterization of cuproptosis-related
molecular clusters in Ulcerative Colitis” conducted by Pu et al. has been evaluated. I think this
study has certain innovation and clinical significance. In this study, the author comprehensively
explored the cuproptosis-related genes in ulcerative colitis. However, some obvious limitations
impair the overall quality. Before it is suitable for publication, I suggest the author make
substantive modifications.
1. However, I noticed that some of the figures have a low resolution which makes them hard
to read. I would suggest that the authors increase the resolution of these figures, which would
make them easier to read and understand. This would also make it easier for readers to fully
understand the results and make use of it. I believe that increasing the resolution of these figures
would greatly improve the manuscript.
2. Some previous studies have used similar methods or statistical methods. Although it will
lose some innovation, I think it will benefit the reader if the authors could include the
following references, which would provide readers with a more complete understanding of the
whole study (PMID: 33850225, PMID: 34099740, PMID: 35277527, PMID: 33223511, PMID:
34358932 ...).
3. Detailed description of all method sections is required.
4. State the * and statistic value in the method section or otherwise mention in every figure
legend. e.g., *p>0.05.
5. Check the reference style and abbreviation of journals.
6. Some typos are there; rectify those (Minor).
7. the relationship between immune cell infiltration and specifc pathways should be well
discussed in the relevant section.
8. The result of figure 9D seems to be over-fitting. Can the author take measures or explain how
he avoids the risk of over-fitting?
Please review this paper.
user
Title: Identification and immunological characterization of cuproptosis-related molecular
clusters in Ulcerative Colitis
Version: 2 Date: 21 Feb 2023
Reviewer's report:
The paper named “Identification and immunological characterization of cuproptosis-related
molecular clusters in Ulcerative Colitis” conducted by Pu et al. has been evaluated. I think this
study has certain innovation and clinical significance. In this study, the author comprehensively
explored the cuproptosis-related genes in ulcerative colitis. However, some obvious limitations
impair the overall quality. Before it is suitable for publication, I suggest the author make
substantive modifications.
1. However, I noticed that some of the figures have a low resolution which makes them hard
to read. I would suggest that the authors increase the resolution of these figures, which would
make them easier to read and understand. This would also make it easier for readers to fully
understand the results and make use of it. I believe that increasing the resolution of these figures
would greatly improve the manuscript.
2. Some previous studies have used similar methods or statistical methods. Although it will
lose some innovation, I think it will benefit the reader if the authors could include the
following references, which would provide readers with a more complete understanding of the
whole study (PMID: 33850225, PMID: 34099740, PMID: 35277527, PMID: 33223511, PMID:
34358932 ...).
3. Detailed description of all method sections is required.
4. State the * and statistic value in the method section or otherwise mention in every figure
legend. e.g., *p>0.05.
5. Check the reference style and abbreviation of journals.
6. Some typos are there; rectify those (Minor).
7. the relationship between immune cell infiltration and specifc pathways should be well
discussed in the relevant section.
8. The result of figure 9D seems to be over-fitting. Can the author take measures or explain how
he avoids the risk of over-fitting?
assistant