Reviewer's report
Title:Effects of mannoprotein E1 in liquid diet on inflammatory response and
TLR5 expression in the gut of rats infected by Salmonella typhimurium
Version: 1Date:12 January 2010
Reviewer: Shizuo Akira
Reviewer's report:
Several lines of evidence support a role for Toll-like receptor (TLR) signaling to
protect the intestine from pathogenic infection. TLR signaling at the level of the
intestinal epithelium is critical for mucosal immune responses. The intestine is
normally colonized by a large and diverse commensal microbiota and is
occasionally exposed to a variety of potential pathogens. One specific molecular
interaction that appears to play a key role in governing bacterial-intestinal
interactions is that of the bacterial protein flagellin with toll-like receptor 5. This
study investigates the effects of mannoprotein administration in a liquid diet on
inflammatory response and TLR5 expression changes during intestinal tissue
injury in a rat model of infection with Salmonella typhimurium. The study has
been well improved according to previous reviewer’s suggestions this time.
Major concerns:
1. Due to the change of their text, evaluation of bacterial translocation (BT) is
now appropriate.
2.After the paper has been reviewed by a gastroenterologist, the terminology
became correct.
3.The mechanisms of flagellin-TLR5 pathway (MYD 88) as well as mechanisms
of proliferatin (ERK) and apoptosis (bax,bcl2, FAS etc) were well-discussed in
the revised manuscript.
Minor concerns:
The authors changed all the points according to the reviewer’s suggestion
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Declaration of competing interests:
I declare that I have no competing interests' below.
Reviewer's report
Title:Effects of mannoprotein E1 in liquid diet on inflammatory response and
TLR5 expression in the gut of rats infected by Salmonella typhimurium
Version: 1Date:12 January 2010
Reviewer: Shizuo Akira
Reviewer's report:
Several lines of evidence support a role for Toll-like receptor (TLR) signaling to
protect the intestine from pathogenic infection. TLR signaling at the level of the
intestinal epithelium is critical for mucosal immune responses. The intestine is
normally colonized by a large and diverse commensal microbiota and is
occasionally exposed to a variety of potential pathogens. One specific molecular
interaction that appears to play a key role in governing bacterial-intestinal
interactions is that of the bacterial protein flagellin with toll-like receptor 5. This
study investigates the effects of mannoprotein administration in a liquid diet on
inflammatory response and TLR5 expression changes during intestinal tissue
injury in a rat model of infection with Salmonella typhimurium. The study has
been well improved according to previous reviewer’s suggestions this time.
Major concerns:
1. Due to the change of their text, evaluation of bacterial translocation (BT) is
now appropriate.
2.After the paper has been reviewed by a gastroenterologist, the terminology
became correct.
3.The mechanisms of flagellin-TLR5 pathway (MYD 88) as well as mechanisms
of proliferatin (ERK) and apoptosis (bax,bcl2, FAS etc) were well-discussed in
the revised manuscript.
Minor concerns:
The authors changed all the points according to the reviewer’s suggestion
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Declaration of competing interests:
I declare that I have no competing interests' below.
Please review this paper.
user
Reviewer's report
Title:Effects of mannoprotein E1 in liquid diet on inflammatory response and
TLR5 expression in the gut of rats infected by Salmonella typhimurium
Version: 1Date:12 January 2010
Reviewer: Shizuo Akira
Reviewer's report:
Several lines of evidence support a role for Toll-like receptor (TLR) signaling to
protect the intestine from pathogenic infection. TLR signaling at the level of the
intestinal epithelium is critical for mucosal immune responses. The intestine is
normally colonized by a large and diverse commensal microbiota and is
occasionally exposed to a variety of potential pathogens. One specific molecular
interaction that appears to play a key role in governing bacterial-intestinal
interactions is that of the bacterial protein flagellin with toll-like receptor 5. This
study investigates the effects of mannoprotein administration in a liquid diet on
inflammatory response and TLR5 expression changes during intestinal tissue
injury in a rat model of infection with Salmonella typhimurium. The study has
been well improved according to previous reviewer’s suggestions this time.
Major concerns:
1. Due to the change of their text, evaluation of bacterial translocation (BT) is
now appropriate.
2.After the paper has been reviewed by a gastroenterologist, the terminology
became correct.
3.The mechanisms of flagellin-TLR5 pathway (MYD 88) as well as mechanisms
of proliferatin (ERK) and apoptosis (bax,bcl2, FAS etc) were well-discussed in
the revised manuscript.
Minor concerns:
The authors changed all the points according to the reviewer’s suggestion
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Declaration of competing interests:
I declare that I have no competing interests' below.
assistant