Title: Elevated tumor expression of Astroprincin (FAM171A1) is an independent marker of
poor prognosis in colon cancer
Version: 5 Date: 20 Jul 2021
Reviewer's report:
Kaprio et al have resubmitted their manuscript on the investigation of APCN in colon cancer. I
appreciate their response to my comments and concerns. In particular, I believe the manuscript
was strengthened by the now included supplementary data on patient information and the
additional analysis on Stage II data. I have three additional improvements that need to be made
that are described below (which should be easy for the authors to address).
1. Please correct the grammar in this sentence that was added to the introduction – it is
incomplete. “Translating biomarkers into clinical use is challenging, in addition to potential
biomarker also large enough patient series, validation series, analytical validation between
laboratories and prospective series in a later phase are needed.”
2. In Figure 1. The authors added a scale bar to Figure 1A, but an appropriate scale bar needs
to be added to every image.
3. Figure 1E was also added and described in the legend as “Colon cancer and tumor-next normal
epithelium” this is worded a bit odd. Perhaps simply “Normal epithelium neighboring a colon
tumor”.
Title: Elevated tumor expression of Astroprincin (FAM171A1) is an independent marker of
poor prognosis in colon cancer
Version: 5 Date: 20 Jul 2021
Reviewer's report:
Kaprio et al have resubmitted their manuscript on the investigation of APCN in colon cancer. I
appreciate their response to my comments and concerns. In particular, I believe the manuscript
was strengthened by the now included supplementary data on patient information and the
additional analysis on Stage II data. I have three additional improvements that need to be made
that are described below (which should be easy for the authors to address).
1. Please correct the grammar in this sentence that was added to the introduction – it is
incomplete. “Translating biomarkers into clinical use is challenging, in addition to potential
biomarker also large enough patient series, validation series, analytical validation between
laboratories and prospective series in a later phase are needed.”
2. In Figure 1. The authors added a scale bar to Figure 1A, but an appropriate scale bar needs
to be added to every image.
3. Figure 1E was also added and described in the legend as “Colon cancer and tumor-next normal
epithelium” this is worded a bit odd. Perhaps simply “Normal epithelium neighboring a colon
tumor”.
Please review this paper.
user
Title: Elevated tumor expression of Astroprincin (FAM171A1) is an independent marker of
poor prognosis in colon cancer
Version: 5 Date: 20 Jul 2021
Reviewer's report:
Kaprio et al have resubmitted their manuscript on the investigation of APCN in colon cancer. I
appreciate their response to my comments and concerns. In particular, I believe the manuscript
was strengthened by the now included supplementary data on patient information and the
additional analysis on Stage II data. I have three additional improvements that need to be made
that are described below (which should be easy for the authors to address).
1. Please correct the grammar in this sentence that was added to the introduction – it is
incomplete. “Translating biomarkers into clinical use is challenging, in addition to potential
biomarker also large enough patient series, validation series, analytical validation between
laboratories and prospective series in a later phase are needed.”
2. In Figure 1. The authors added a scale bar to Figure 1A, but an appropriate scale bar needs
to be added to every image.
3. Figure 1E was also added and described in the legend as “Colon cancer and tumor-next normal
epithelium” this is worded a bit odd. Perhaps simply “Normal epithelium neighboring a colon
tumor”.
assistant