Reviewer's report
Title:Transcription factors GATA-4 and GATA-6 in normal and neoplastic human
gastrointestinal mucosa
Version: 2Date:17 December 2007
Reviewer: Kentaro Sugano
Reviewer's report:
The most of the points raised by this reviewer were answered by authors.
However, the reason why they chose to use anti-Na+-K+-ATPase (alfa subunit)to
identify parietal cells is not well understood, because the antibody to either alfa or
beta subunit to H+-K-ATPase is commercally availble.
As authors commented that the localization of Na+-K+-ATPase (alfa subunit)was
in the basolateral membrane, it is obvious that the antibody did not recognize
H+-K-ATPase which is located in the cytoplasm (microvesicles) or luminal
membrane.
Thus, the identity of parietal cells should be re-confirmed with the specific
antibody to H+, K+-ATPase.
What next?: Accept after minor essential revisions
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
No conflicts of interests exist.
Reviewer's report
Title:Transcription factors GATA-4 and GATA-6 in normal and neoplastic human
gastrointestinal mucosa
Version: 2Date:17 December 2007
Reviewer: Kentaro Sugano
Reviewer's report:
The most of the points raised by this reviewer were answered by authors.
However, the reason why they chose to use anti-Na+-K+-ATPase (alfa subunit)to
identify parietal cells is not well understood, because the antibody to either alfa or
beta subunit to H+-K-ATPase is commercally availble.
As authors commented that the localization of Na+-K+-ATPase (alfa subunit)was
in the basolateral membrane, it is obvious that the antibody did not recognize
H+-K-ATPase which is located in the cytoplasm (microvesicles) or luminal
membrane.
Thus, the identity of parietal cells should be re-confirmed with the specific
antibody to H+, K+-ATPase.
What next?: Accept after minor essential revisions
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
No conflicts of interests exist.
Please review this paper.
user
Reviewer's report
Title:Transcription factors GATA-4 and GATA-6 in normal and neoplastic human
gastrointestinal mucosa
Version: 2Date:17 December 2007
Reviewer: Kentaro Sugano
Reviewer's report:
The most of the points raised by this reviewer were answered by authors.
However, the reason why they chose to use anti-Na+-K+-ATPase (alfa subunit)to
identify parietal cells is not well understood, because the antibody to either alfa or
beta subunit to H+-K-ATPase is commercally availble.
As authors commented that the localization of Na+-K+-ATPase (alfa subunit)was
in the basolateral membrane, it is obvious that the antibody did not recognize
H+-K-ATPase which is located in the cytoplasm (microvesicles) or luminal
membrane.
Thus, the identity of parietal cells should be re-confirmed with the specific
antibody to H+, K+-ATPase.
What next?: Accept after minor essential revisions
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
No conflicts of interests exist.
assistant