Title: Correlation analyses of ultrasonographic and histopathological characteristics of porta
hepatis lymph nodes in biliary atresia
Version: 0 Date: 28 Jan 2025
Reviewer's report:
Authors provided sufficient information in material and methods for the analyses of the
correlation of ultrasonographic and histopathological characters of porta hepatic LNs.
-It is quite odd to provide a control group with completely different features in all aspects from
the study group in terms of age, gender and laboratory data. The fact that the control group has
choledochal cyst and the study group has biliary atresia which BA demonstrated significantly
more hepatic fibrosis and lymphocyte infiltration in all cell types seemed straight forward, is
there a reason to compare these two groups?
-The manuscript referred to supplementary table 1-8 whereas the supplementary tables were
named S1-S8.
-Table S2 "(days)" should be after "Age"
-Table S3 named "Pathological features of hepatic portal LNs in the BA group" while PHLN
(porta hepatis LN) throughout the manuscript.
-The abbreviations and their meanings used : , ; inconsistently.
-In the study of degrees of lymphocyte infiltration, most tables used "Manifold area" is that the
same as portal area used in the manuscript?
-Manuscript line 251-252 misspelled "fibrosis"
-It is interesting to find larger areas and higher numbers of germinal centers in PHLNs, along
with a high number ofCD8+ T lymphocytes in the livers of patients with BA correlating to
a higher degree of liver fibrosis and inflammation. Also that a greater number of CD8+ T
lymphocytes in the livers of patients with BA associated with a  worsen jaundice reduction after
surgery.  All of which differed from previous studies by Zhang et al, Ahmed et al. and Derkow et
al. On the contrary, according to supplementary table S6, CD3+ and CD4+ T cells and CD20+ B
cells were more infiltrated in the portal area than in the hepatic lobes which portal area is where
the bile ducts were destroyed. Any comments on these differences?
-CD8+ T cells and pathological size of the PHLNs were the only 2 factors associated with
poor prognosis after portoenterostomy, however the authors concluded that the enlarged PHLN
may predict the prognosis of KPE which the ultrasonographic size did not show significant
association.
-Some of the reference paragraphs were distributed too wide.
Title: Correlation analyses of ultrasonographic and histopathological characteristics of porta
hepatis lymph nodes in biliary atresia
Version: 0 Date: 28 Jan 2025
Reviewer's report:
Authors provided sufficient information in material and methods for the analyses of the
correlation of ultrasonographic and histopathological characters of porta hepatic LNs.
-It is quite odd to provide a control group with completely different features in all aspects from
the study group in terms of age, gender and laboratory data. The fact that the control group has
choledochal cyst and the study group has biliary atresia which BA demonstrated significantly
more hepatic fibrosis and lymphocyte infiltration in all cell types seemed straight forward, is
there a reason to compare these two groups?
-The manuscript referred to supplementary table 1-8 whereas the supplementary tables were
named S1-S8.
-Table S2 "(days)" should be after "Age"
-Table S3 named "Pathological features of hepatic portal LNs in the BA group" while PHLN
(porta hepatis LN) throughout the manuscript.
-The abbreviations and their meanings used : , ; inconsistently.
-In the study of degrees of lymphocyte infiltration, most tables used "Manifold area" is that the
same as portal area used in the manuscript?
-Manuscript line 251-252 misspelled "fibrosis"
-It is interesting to find larger areas and higher numbers of germinal centers in PHLNs, along
with a high number ofCD8+ T lymphocytes in the livers of patients with BA correlating to
a higher degree of liver fibrosis and inflammation. Also that a greater number of CD8+ T
lymphocytes in the livers of patients with BA associated with a  worsen jaundice reduction after
surgery.  All of which differed from previous studies by Zhang et al, Ahmed et al. and Derkow et
al. On the contrary, according to supplementary table S6, CD3+ and CD4+ T cells and CD20+ B
cells were more infiltrated in the portal area than in the hepatic lobes which portal area is where
the bile ducts were destroyed. Any comments on these differences?
-CD8+ T cells and pathological size of the PHLNs were the only 2 factors associated with
poor prognosis after portoenterostomy, however the authors concluded that the enlarged PHLN
may predict the prognosis of KPE which the ultrasonographic size did not show significant
association.
-Some of the reference paragraphs were distributed too wide.
Please review this paper.
user
Title: Correlation analyses of ultrasonographic and histopathological characteristics of porta
hepatis lymph nodes in biliary atresia
Version: 0 Date: 28 Jan 2025
Reviewer's report:
Authors provided sufficient information in material and methods for the analyses of the
correlation of ultrasonographic and histopathological characters of porta hepatic LNs.
-It is quite odd to provide a control group with completely different features in all aspects from
the study group in terms of age, gender and laboratory data. The fact that the control group has
choledochal cyst and the study group has biliary atresia which BA demonstrated significantly
more hepatic fibrosis and lymphocyte infiltration in all cell types seemed straight forward, is
there a reason to compare these two groups?
-The manuscript referred to supplementary table 1-8 whereas the supplementary tables were
named S1-S8.
-Table S2 "(days)" should be after "Age"
-Table S3 named "Pathological features of hepatic portal LNs in the BA group" while PHLN
(porta hepatis LN) throughout the manuscript.
-The abbreviations and their meanings used : , ; inconsistently.
-In the study of degrees of lymphocyte infiltration, most tables used "Manifold area" is that the
same as portal area used in the manuscript?
-Manuscript line 251-252 misspelled "fibrosis"
-It is interesting to find larger areas and higher numbers of germinal centers in PHLNs, along
with a high number ofCD8+ T lymphocytes in the livers of patients with BA correlating to
a higher degree of liver fibrosis and inflammation. Also that a greater number of CD8+ T
lymphocytes in the livers of patients with BA associated with a  worsen jaundice reduction after
surgery.  All of which differed from previous studies by Zhang et al, Ahmed et al. and Derkow et
al. On the contrary, according to supplementary table S6, CD3+ and CD4+ T cells and CD20+ B
cells were more infiltrated in the portal area than in the hepatic lobes which portal area is where
the bile ducts were destroyed. Any comments on these differences?
-CD8+ T cells and pathological size of the PHLNs were the only 2 factors associated with
poor prognosis after portoenterostomy, however the authors concluded that the enlarged PHLN
may predict the prognosis of KPE which the ultrasonographic size did not show significant
association.
-Some of the reference paragraphs were distributed too wide.
assistant