Reviewer's report
Title:Moderate performance of serum S100A12, in distinguishing inflammatory
bowel disease from irritable bowel syndrome
Version: 2Date:2 August 2010
Reviewer: Dirk Foell
Reviewer's report:
Although confirmed by the manufacturer of the ELISA assay, the problems of the
ELISA are not mentioned at all in the revised manuscript. As CISbio confirmed,
the assay will never be launched for blood analyses. It is not sufficient to state
the pre-analytical steps in the methods section. Instead, the limitations of the
used assay should be thoroughly discussed in the context of existing reports on
studies using at least two independent ELISAs which work in blood samples,
obviously.
It is unacceptable that the authors did not follow the advice to at least discuss
this issue (and that is really a minimum requirement).
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
As previously stated
Reviewer's report
Title:Moderate performance of serum S100A12, in distinguishing inflammatory
bowel disease from irritable bowel syndrome
Version: 2Date:2 August 2010
Reviewer: Dirk Foell
Reviewer's report:
Although confirmed by the manufacturer of the ELISA assay, the problems of the
ELISA are not mentioned at all in the revised manuscript. As CISbio confirmed,
the assay will never be launched for blood analyses. It is not sufficient to state
the pre-analytical steps in the methods section. Instead, the limitations of the
used assay should be thoroughly discussed in the context of existing reports on
studies using at least two independent ELISAs which work in blood samples,
obviously.
It is unacceptable that the authors did not follow the advice to at least discuss
this issue (and that is really a minimum requirement).
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
As previously stated
Please review this paper.
user
Reviewer's report
Title:Moderate performance of serum S100A12, in distinguishing inflammatory
bowel disease from irritable bowel syndrome
Version: 2Date:2 August 2010
Reviewer: Dirk Foell
Reviewer's report:
Although confirmed by the manufacturer of the ELISA assay, the problems of the
ELISA are not mentioned at all in the revised manuscript. As CISbio confirmed,
the assay will never be launched for blood analyses. It is not sufficient to state
the pre-analytical steps in the methods section. Instead, the limitations of the
used assay should be thoroughly discussed in the context of existing reports on
studies using at least two independent ELISAs which work in blood samples,
obviously.
It is unacceptable that the authors did not follow the advice to at least discuss
this issue (and that is really a minimum requirement).
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
As previously stated
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