Reviewer's report
Title:High mobility group B1 impairs hepatocyte regeneration in acetaminophen
hepatotoxicity
Version: 3Date:26 March 2012
Reviewer: Leo A van Grunsven
Reviewer's report:
Yang et al.
High mobility group B1 impairs hepatocyte regeneration in acetaminophen
Hepatotoxicity
Authors have addressed all comments and modified the manuscript accordingly
and is suitable for publication in BMG.
Minor comments on their changes and letter:
The authors make a strong point that NF-kB bandshift withouth supershift or cold
competition are informative in this study. Their comment on my request was
“Nowadays, most of the papers and journals no longer require NF-kB cold
competition and supershift tests”
This is probably true because the standard nowadays is ChIP, nobody bothers
with EMSA anymore due to its low predictability of the real binding situation of
the protein of interest. It gives the potential of the protein to bind and in this
manuscript it indicates the amount of activated NF-kB present in the
ant-HMGB-1-2 injected animals. Since this is the only claim that the authors
make “activation of NF-kB by blockade of HMGB1” the experiment is valid and
rightly used.
Figure 6 has been added, but the legend is not very clear, i.e. it is not clear what
is A, B or C in the figure.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:High mobility group B1 impairs hepatocyte regeneration in acetaminophen
hepatotoxicity
Version: 3Date:26 March 2012
Reviewer: Leo A van Grunsven
Reviewer's report:
Yang et al.
High mobility group B1 impairs hepatocyte regeneration in acetaminophen
Hepatotoxicity
Authors have addressed all comments and modified the manuscript accordingly
and is suitable for publication in BMG.
Minor comments on their changes and letter:
The authors make a strong point that NF-kB bandshift withouth supershift or cold
competition are informative in this study. Their comment on my request was
“Nowadays, most of the papers and journals no longer require NF-kB cold
competition and supershift tests”
This is probably true because the standard nowadays is ChIP, nobody bothers
with EMSA anymore due to its low predictability of the real binding situation of
the protein of interest. It gives the potential of the protein to bind and in this
manuscript it indicates the amount of activated NF-kB present in the
ant-HMGB-1-2 injected animals. Since this is the only claim that the authors
make “activation of NF-kB by blockade of HMGB1” the experiment is valid and
rightly used.
Figure 6 has been added, but the legend is not very clear, i.e. it is not clear what
is A, B or C in the figure.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:High mobility group B1 impairs hepatocyte regeneration in acetaminophen
hepatotoxicity
Version: 3Date:26 March 2012
Reviewer: Leo A van Grunsven
Reviewer's report:
Yang et al.
High mobility group B1 impairs hepatocyte regeneration in acetaminophen
Hepatotoxicity
Authors have addressed all comments and modified the manuscript accordingly
and is suitable for publication in BMG.
Minor comments on their changes and letter:
The authors make a strong point that NF-kB bandshift withouth supershift or cold
competition are informative in this study. Their comment on my request was
“Nowadays, most of the papers and journals no longer require NF-kB cold
competition and supershift tests”
This is probably true because the standard nowadays is ChIP, nobody bothers
with EMSA anymore due to its low predictability of the real binding situation of
the protein of interest. It gives the potential of the protein to bind and in this
manuscript it indicates the amount of activated NF-kB present in the
ant-HMGB-1-2 injected animals. Since this is the only claim that the authors
make “activation of NF-kB by blockade of HMGB1” the experiment is valid and
rightly used.
Figure 6 has been added, but the legend is not very clear, i.e. it is not clear what
is A, B or C in the figure.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
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