Title: Bacterial and Clinical Metabolic Signatures and Their Interactions in Obese Patients Post-
Bariatric Surgery
Version: 5 Date: 02 Sep 2024
Reviewer's report:
The authors evaluate the changes and effects of intestinal microbiota after laparoscopic sleeve
gastrectomy (LSG). They found that LSG reduced patients' BMI and altered the composition
of the intestinal microbiota in obese patients in China. However, many studies also showed
significant gut microbiota change after bariatric surgery already.
Comment
1. You can have three groups for comparison, obesity before LSG, after LSG, and normal BMI
control.
2. Do you have the data of stool metabolites before and after LSG, it is also very important in
these bariatric patients. They are strong associated with or related to gut microbiota.
3. Do you have the data of gut hormones like GLP-1, PYY before and after LSG, they may have
changed after surgery.
4. Diet can modify the body weight, gut microbiota, do you record the diet pattern, food amount
before and after LSG ?
5. Are you sure it is 16srDNA rather than 16srRNA in DNA analysis of the stool samples ?
6. Do you have the legends for figure 2-D.
7. In the discussion, you described that there are significant population differences in species-
level diversity, the analysis of V3,V4 16srRNA in your study cannot discrete the gut microbiota
into species-level.
8. In the legend of figure 4, “black indicates positive correlation, and red indicates negative
correlation. The size of the circles represents the magnitude of the correlation, with larger circles
indicating higher correlation values.” What are higher correlation values, for example r=0.7 or
0.8, please quantify them.
Title: Bacterial and Clinical Metabolic Signatures and Their Interactions in Obese Patients Post-
Bariatric Surgery
Version: 5 Date: 02 Sep 2024
Reviewer's report:
The authors evaluate the changes and effects of intestinal microbiota after laparoscopic sleeve
gastrectomy (LSG). They found that LSG reduced patients' BMI and altered the composition
of the intestinal microbiota in obese patients in China. However, many studies also showed
significant gut microbiota change after bariatric surgery already.
Comment
1. You can have three groups for comparison, obesity before LSG, after LSG, and normal BMI
control.
2. Do you have the data of stool metabolites before and after LSG, it is also very important in
these bariatric patients. They are strong associated with or related to gut microbiota.
3. Do you have the data of gut hormones like GLP-1, PYY before and after LSG, they may have
changed after surgery.
4. Diet can modify the body weight, gut microbiota, do you record the diet pattern, food amount
before and after LSG ?
5. Are you sure it is 16srDNA rather than 16srRNA in DNA analysis of the stool samples ?
6. Do you have the legends for figure 2-D.
7. In the discussion, you described that there are significant population differences in species-
level diversity, the analysis of V3,V4 16srRNA in your study cannot discrete the gut microbiota
into species-level.
8. In the legend of figure 4, “black indicates positive correlation, and red indicates negative
correlation. The size of the circles represents the magnitude of the correlation, with larger circles
indicating higher correlation values.” What are higher correlation values, for example r=0.7 or
0.8, please quantify them.
Please review this paper.
user
Title: Bacterial and Clinical Metabolic Signatures and Their Interactions in Obese Patients Post-
Bariatric Surgery
Version: 5 Date: 02 Sep 2024
Reviewer's report:
The authors evaluate the changes and effects of intestinal microbiota after laparoscopic sleeve
gastrectomy (LSG). They found that LSG reduced patients' BMI and altered the composition
of the intestinal microbiota in obese patients in China. However, many studies also showed
significant gut microbiota change after bariatric surgery already.
Comment
1. You can have three groups for comparison, obesity before LSG, after LSG, and normal BMI
control.
2. Do you have the data of stool metabolites before and after LSG, it is also very important in
these bariatric patients. They are strong associated with or related to gut microbiota.
3. Do you have the data of gut hormones like GLP-1, PYY before and after LSG, they may have
changed after surgery.
4. Diet can modify the body weight, gut microbiota, do you record the diet pattern, food amount
before and after LSG ?
5. Are you sure it is 16srDNA rather than 16srRNA in DNA analysis of the stool samples ?
6. Do you have the legends for figure 2-D.
7. In the discussion, you described that there are significant population differences in species-
level diversity, the analysis of V3,V4 16srRNA in your study cannot discrete the gut microbiota
into species-level.
8. In the legend of figure 4, “black indicates positive correlation, and red indicates negative
correlation. The size of the circles represents the magnitude of the correlation, with larger circles
indicating higher correlation values.” What are higher correlation values, for example r=0.7 or
0.8, please quantify them.
assistant