Reviewer's report
Title:Clostridium leptum group bacteria abundance and diversity in the fecal
microbiota of patients with inflammatory bowel disease: a case-control study in
India
Version: 1Date:14 October 2012
Reviewer: liping chen
Reviewer's report:
C.leptum group, which belongs to the Firmicutes phylum, can produce short
chain fatty acid exerting anti-inflammatory effect. This study explored the
characteristics of C.leptum group in Indian IBD patients by TTGE and qPCR. It is
interesting and important for us to improve our understanding about dysboisis of
bacterial subgroups in IBD patients with different geographical background.
In this study, authors draw a conclusion that total numbers of C.leptum group
bacteria and F.prausnitzii was reduced in both CD and UC patients, and were not
related with disease activity.
I think it is debatable in that author did not characterize the number of total
bacteria. The premise to compare quantity variance of bacterium (group)
between IBD and healthy controls is that total bacterial number is comparative
between IBD and healthy controls. But i have no information about it from this
study. I think it is reasonable to compare bacterium (group) in different groups
with ratio of some bacterium (group) and the number of total bacteria. I suggests
that author characterizes the number of total bacteria furtherly.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
Declaration of competing interests:
I declare that i have no competing interests
Reviewer's report
Title:Clostridium leptum group bacteria abundance and diversity in the fecal
microbiota of patients with inflammatory bowel disease: a case-control study in
India
Version: 1Date:14 October 2012
Reviewer: liping chen
Reviewer's report:
C.leptum group, which belongs to the Firmicutes phylum, can produce short
chain fatty acid exerting anti-inflammatory effect. This study explored the
characteristics of C.leptum group in Indian IBD patients by TTGE and qPCR. It is
interesting and important for us to improve our understanding about dysboisis of
bacterial subgroups in IBD patients with different geographical background.
In this study, authors draw a conclusion that total numbers of C.leptum group
bacteria and F.prausnitzii was reduced in both CD and UC patients, and were not
related with disease activity.
I think it is debatable in that author did not characterize the number of total
bacteria. The premise to compare quantity variance of bacterium (group)
between IBD and healthy controls is that total bacterial number is comparative
between IBD and healthy controls. But i have no information about it from this
study. I think it is reasonable to compare bacterium (group) in different groups
with ratio of some bacterium (group) and the number of total bacteria. I suggests
that author characterizes the number of total bacteria furtherly.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
Declaration of competing interests:
I declare that i have no competing interests
Please review this paper.
user
Reviewer's report
Title:Clostridium leptum group bacteria abundance and diversity in the fecal
microbiota of patients with inflammatory bowel disease: a case-control study in
India
Version: 1Date:14 October 2012
Reviewer: liping chen
Reviewer's report:
C.leptum group, which belongs to the Firmicutes phylum, can produce short
chain fatty acid exerting anti-inflammatory effect. This study explored the
characteristics of C.leptum group in Indian IBD patients by TTGE and qPCR. It is
interesting and important for us to improve our understanding about dysboisis of
bacterial subgroups in IBD patients with different geographical background.
In this study, authors draw a conclusion that total numbers of C.leptum group
bacteria and F.prausnitzii was reduced in both CD and UC patients, and were not
related with disease activity.
I think it is debatable in that author did not characterize the number of total
bacteria. The premise to compare quantity variance of bacterium (group)
between IBD and healthy controls is that total bacterial number is comparative
between IBD and healthy controls. But i have no information about it from this
study. I think it is reasonable to compare bacterium (group) in different groups
with ratio of some bacterium (group) and the number of total bacteria. I suggests
that author characterizes the number of total bacteria furtherly.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
Declaration of competing interests:
I declare that i have no competing interests
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