Title:  Immunohistochemical identification and assessment of the location of leptin, visfatin and
chemerin in the liver of men with different body mass index
Version: 1 Date: 21 Jun 2021
Reviewer's report:
The authors present a small study that evaluated the immunohistochemistry and mRNA
expression of 3 adipokines (leptin, visfatin and chemerin) in the liver of 14 liver organ-donors.
The study is interesting and original, but it should either be presented as a short communication/
letter or it needs further developments:
1) Was the number of patients necessary calculated or was this a convenience sample?
2) The immunohistochemistry/mRNA expression data should be controlled/correlated with
different variables: age, abdominal circumference, liver enzymes, serum levels of the studied
adipokines, adipose tissue expression, glucose levels/insulin resistance, serum lipid profile,
quantification of liver steatosis, inflammation or fibrosis.
Furthermore, I have real concerns regarding the immunohistochemistry of leptin. It may simply
reflect unspecific staining.
How do you explain the different results in immunohistochemistry and mRNA expression of
chemerin?
Title:  Immunohistochemical identification and assessment of the location of leptin, visfatin and
chemerin in the liver of men with different body mass index
Version: 1 Date: 21 Jun 2021
Reviewer's report:
The authors present a small study that evaluated the immunohistochemistry and mRNA
expression of 3 adipokines (leptin, visfatin and chemerin) in the liver of 14 liver organ-donors.
The study is interesting and original, but it should either be presented as a short communication/
letter or it needs further developments:
1) Was the number of patients necessary calculated or was this a convenience sample?
2) The immunohistochemistry/mRNA expression data should be controlled/correlated with
different variables: age, abdominal circumference, liver enzymes, serum levels of the studied
adipokines, adipose tissue expression, glucose levels/insulin resistance, serum lipid profile,
quantification of liver steatosis, inflammation or fibrosis.
Furthermore, I have real concerns regarding the immunohistochemistry of leptin. It may simply
reflect unspecific staining.
How do you explain the different results in immunohistochemistry and mRNA expression of
chemerin?
Please review this paper.
user
Title:  Immunohistochemical identification and assessment of the location of leptin, visfatin and
chemerin in the liver of men with different body mass index
Version: 1 Date: 21 Jun 2021
Reviewer's report:
The authors present a small study that evaluated the immunohistochemistry and mRNA
expression of 3 adipokines (leptin, visfatin and chemerin) in the liver of 14 liver organ-donors.
The study is interesting and original, but it should either be presented as a short communication/
letter or it needs further developments:
1) Was the number of patients necessary calculated or was this a convenience sample?
2) The immunohistochemistry/mRNA expression data should be controlled/correlated with
different variables: age, abdominal circumference, liver enzymes, serum levels of the studied
adipokines, adipose tissue expression, glucose levels/insulin resistance, serum lipid profile,
quantification of liver steatosis, inflammation or fibrosis.
Furthermore, I have real concerns regarding the immunohistochemistry of leptin. It may simply
reflect unspecific staining.
How do you explain the different results in immunohistochemistry and mRNA expression of
chemerin?
assistant