Title: First Report on Molecular Docking Analysis and Drug Resistance Substitutions to
Approved HCV NS5A and NS5B Inhibitors amongst Iranian Patients
Version: 3 Date: 31 Aug 2021
Reviewer's report:
The fact that the expected phosphorylation amino acids play a critical role in viral replication,
pathogenesis and liver disease should be demonstrated by clinical data and not just affirmed on
the basis of mere hypotheses.
I do not understand the role in the discussino of the issue concerning yeast and E.Coli NS5A
and NS5B protein expression; where does this want to lead?
In general the biochemical part of the discussion is written in very complicated , confusing and
inconclusive way and makes the pathogenetic  aspects  difficult to understand.
The explanation of the great difference between the mutations found by the authors and other
studies must  be discussed in depth. Numerical, geographical, clinical and genetic aspects in
other studies should be evaluated and reported in the discussion not generally suggested.
References in the introduction should be corrected because they are often not relevant (REF 1,
2, 3, 4)
The manuscript needs to be streamlined and made easier to read.
Methods:
What was the detection limit of the test? How many copies of HCV?
Sanger Sequencing methods are not reported
The table two reports the mutations in NS5a and NS5B; mutations at positions 62, 309 and 327
in NS5a are reported in the discussion.
The table seems to show mutations 309 and 327 in NS5B.... Is that so?
The paper must be corrected in typing and English
Title: First Report on Molecular Docking Analysis and Drug Resistance Substitutions to
Approved HCV NS5A and NS5B Inhibitors amongst Iranian Patients
Version: 3 Date: 31 Aug 2021
Reviewer's report:
The fact that the expected phosphorylation amino acids play a critical role in viral replication,
pathogenesis and liver disease should be demonstrated by clinical data and not just affirmed on
the basis of mere hypotheses.
I do not understand the role in the discussino of the issue concerning yeast and E.Coli NS5A
and NS5B protein expression; where does this want to lead?
In general the biochemical part of the discussion is written in very complicated , confusing and
inconclusive way and makes the pathogenetic  aspects  difficult to understand.
The explanation of the great difference between the mutations found by the authors and other
studies must  be discussed in depth. Numerical, geographical, clinical and genetic aspects in
other studies should be evaluated and reported in the discussion not generally suggested.
References in the introduction should be corrected because they are often not relevant (REF 1,
2, 3, 4)
The manuscript needs to be streamlined and made easier to read.
Methods:
What was the detection limit of the test? How many copies of HCV?
Sanger Sequencing methods are not reported
The table two reports the mutations in NS5a and NS5B; mutations at positions 62, 309 and 327
in NS5a are reported in the discussion.
The table seems to show mutations 309 and 327 in NS5B.... Is that so?
The paper must be corrected in typing and English
Please review this paper.
user
Title: First Report on Molecular Docking Analysis and Drug Resistance Substitutions to
Approved HCV NS5A and NS5B Inhibitors amongst Iranian Patients
Version: 3 Date: 31 Aug 2021
Reviewer's report:
The fact that the expected phosphorylation amino acids play a critical role in viral replication,
pathogenesis and liver disease should be demonstrated by clinical data and not just affirmed on
the basis of mere hypotheses.
I do not understand the role in the discussino of the issue concerning yeast and E.Coli NS5A
and NS5B protein expression; where does this want to lead?
In general the biochemical part of the discussion is written in very complicated , confusing and
inconclusive way and makes the pathogenetic  aspects  difficult to understand.
The explanation of the great difference between the mutations found by the authors and other
studies must  be discussed in depth. Numerical, geographical, clinical and genetic aspects in
other studies should be evaluated and reported in the discussion not generally suggested.
References in the introduction should be corrected because they are often not relevant (REF 1,
2, 3, 4)
The manuscript needs to be streamlined and made easier to read.
Methods:
What was the detection limit of the test? How many copies of HCV?
Sanger Sequencing methods are not reported
The table two reports the mutations in NS5a and NS5B; mutations at positions 62, 309 and 327
in NS5a are reported in the discussion.
The table seems to show mutations 309 and 327 in NS5B.... Is that so?
The paper must be corrected in typing and English
assistant