Reviewer's report
Title:Combined effect of regulatory polymorphisms on transcription of UGT1A1
as a cause of Gilbert syndrome
Version: 2Date:10 February 2010
Reviewer: Ching-Shan Huang
Reviewer's report:
1. Are all the SNPs found in the subjects suffering from Gilbert syndrome also
observed in the normal controls? If the answer is yes, “mutation” should be
converted to “variation” at all.
2. Page 15: “Our results for A(TA)7TAA and c.-3275T>G are consistent with the
fact that some homozygotes with A(TA)7TAA do not suffer from Gilbert syndrome
[27,28], and that there are patients with homozygous c.-3275T>G but without
A(TA)7TAA [25].” The following article may be considered to be cited as an
additional reference: Huang YY, Huang MJ, Yang SS, Teng HC, Huang CS:
Variations in the UDP- glucuronosyltransferase 1A1 gene for the development of
unconjugated hyperbilirubinemia in Taiwanese. Pharmacogenomics 2008,
9:1229-1235.
3. Page 16: “Our study shows that -3275T>G and the ten polymorphisms
detected in the patients are not pppless important in their effect on transcription
than A(TA)7TAA, and that Gilbert syndrome is likely to be caused by the
combined effects of these polymorphisms.” Is “pppless” a mistake? Would it be
“less”?
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Combined effect of regulatory polymorphisms on transcription of UGT1A1
as a cause of Gilbert syndrome
Version: 2Date:10 February 2010
Reviewer: Ching-Shan Huang
Reviewer's report:
1. Are all the SNPs found in the subjects suffering from Gilbert syndrome also
observed in the normal controls? If the answer is yes, “mutation” should be
converted to “variation” at all.
2. Page 15: “Our results for A(TA)7TAA and c.-3275T>G are consistent with the
fact that some homozygotes with A(TA)7TAA do not suffer from Gilbert syndrome
[27,28], and that there are patients with homozygous c.-3275T>G but without
A(TA)7TAA [25].” The following article may be considered to be cited as an
additional reference: Huang YY, Huang MJ, Yang SS, Teng HC, Huang CS:
Variations in the UDP- glucuronosyltransferase 1A1 gene for the development of
unconjugated hyperbilirubinemia in Taiwanese. Pharmacogenomics 2008,
9:1229-1235.
3. Page 16: “Our study shows that -3275T>G and the ten polymorphisms
detected in the patients are not pppless important in their effect on transcription
than A(TA)7TAA, and that Gilbert syndrome is likely to be caused by the
combined effects of these polymorphisms.” Is “pppless” a mistake? Would it be
“less”?
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Combined effect of regulatory polymorphisms on transcription of UGT1A1
as a cause of Gilbert syndrome
Version: 2Date:10 February 2010
Reviewer: Ching-Shan Huang
Reviewer's report:
1. Are all the SNPs found in the subjects suffering from Gilbert syndrome also
observed in the normal controls? If the answer is yes, “mutation” should be
converted to “variation” at all.
2. Page 15: “Our results for A(TA)7TAA and c.-3275T>G are consistent with the
fact that some homozygotes with A(TA)7TAA do not suffer from Gilbert syndrome
[27,28], and that there are patients with homozygous c.-3275T>G but without
A(TA)7TAA [25].” The following article may be considered to be cited as an
additional reference: Huang YY, Huang MJ, Yang SS, Teng HC, Huang CS:
Variations in the UDP- glucuronosyltransferase 1A1 gene for the development of
unconjugated hyperbilirubinemia in Taiwanese. Pharmacogenomics 2008,
9:1229-1235.
3. Page 16: “Our study shows that -3275T>G and the ten polymorphisms
detected in the patients are not pppless important in their effect on transcription
than A(TA)7TAA, and that Gilbert syndrome is likely to be caused by the
combined effects of these polymorphisms.” Is “pppless” a mistake? Would it be
“less”?
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
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