Title: Behavioral abnormalities in C57BL/6 mice with chronic ulcerative colitis induced by DSS
Version: 1 Date: 15 Jan 2023
Reviewer's report:
We often encounter patients with ulcerative colitis who are mentally stressed or prone to
mental disorders. It is very interesting that the author reported the association between intestinal
inflammation, behavioral abnormalities, and the hippocampus using an experimental animal
model mouse.
I have a major or several minor questions with the report that the author has presented.
1) Should the authors do an objective scoring of enteritis and hippocampal pathology. Frankly,
I do not see any difference between the CONTROL and DSS groups. To substantiate this, an
objective score should be provided.
At the risk of repeating myself, the author notes in his discussion that the hippocampal pathology
in the 3% DSS group was not significantly different versus the control group. How does the
author conclude that there was no significant difference, something that is not quantified?
2) Does the unpaired t test (two-tailed) mentioned in the statistical methods section mean the
same thing as the T test (and nonparametric tests)? Would it be different? I suggest you correct
it, as it is difficult for the reader to understand. Or please check with a statistician.
3) (Fig 3 e) If you found a significant difference in sucrose water consumption, the author should
show it on the graph. Was there a significant decrease in sucrose water drinking? Or was there
no significant difference in it?
4) In the conclusion, the author states that both the acute and chronic enteritis models
cause behavioral abnormalities. In the acute enteritis model, enteritis symptoms cause
behavioral abnormalities. In the chronic enteritis model, psychological stress causes behavioral
abnormalities. However, based on the results presented by the authors, can we say that in the
chronic enteritis model, enteritis symptoms are not the cause?
5) The sucrose preference test; what does it mean that the acute enteritis model has reduced
preference and the chronic enteritis model does not? The authors should discuss that in the
discussion.
6) In the discussion, "Therefore, we speculated whether the mice in the DSS-induced UC model
had behavioral abnormalities, and hoped to improve the However, there is no experimental
system (whether or not enteritis is improved) in which a therapeutic drug (antipsychotic) was
used on mice in the DSS-induced UC model in this study. There is a disconnect between the
objectives and conclusions.
7) What the author states in the conclusion of the abstract is different from what is presented
in the results. Didn't both models, acute and chronic enteritis models, cause behavioral
abnormalities? Don't you want to state that the causes of the behavioral abnormalities are
different? The abstract should correct the conclusions in the body of the text.
Title: Behavioral abnormalities in C57BL/6 mice with chronic ulcerative colitis induced by DSS
Version: 1 Date: 15 Jan 2023
Reviewer's report:
We often encounter patients with ulcerative colitis who are mentally stressed or prone to
mental disorders. It is very interesting that the author reported the association between intestinal
inflammation, behavioral abnormalities, and the hippocampus using an experimental animal
model mouse.
I have a major or several minor questions with the report that the author has presented.
1) Should the authors do an objective scoring of enteritis and hippocampal pathology. Frankly,
I do not see any difference between the CONTROL and DSS groups. To substantiate this, an
objective score should be provided.
At the risk of repeating myself, the author notes in his discussion that the hippocampal pathology
in the 3% DSS group was not significantly different versus the control group. How does the
author conclude that there was no significant difference, something that is not quantified?
2) Does the unpaired t test (two-tailed) mentioned in the statistical methods section mean the
same thing as the T test (and nonparametric tests)? Would it be different? I suggest you correct
it, as it is difficult for the reader to understand. Or please check with a statistician.
3) (Fig 3 e) If you found a significant difference in sucrose water consumption, the author should
show it on the graph. Was there a significant decrease in sucrose water drinking? Or was there
no significant difference in it?
4) In the conclusion, the author states that both the acute and chronic enteritis models
cause behavioral abnormalities. In the acute enteritis model, enteritis symptoms cause
behavioral abnormalities. In the chronic enteritis model, psychological stress causes behavioral
abnormalities. However, based on the results presented by the authors, can we say that in the
chronic enteritis model, enteritis symptoms are not the cause?
5) The sucrose preference test; what does it mean that the acute enteritis model has reduced
preference and the chronic enteritis model does not? The authors should discuss that in the
discussion.
6) In the discussion, "Therefore, we speculated whether the mice in the DSS-induced UC model
had behavioral abnormalities, and hoped to improve the However, there is no experimental
system (whether or not enteritis is improved) in which a therapeutic drug (antipsychotic) was
used on mice in the DSS-induced UC model in this study. There is a disconnect between the
objectives and conclusions.
7) What the author states in the conclusion of the abstract is different from what is presented
in the results. Didn't both models, acute and chronic enteritis models, cause behavioral
abnormalities? Don't you want to state that the causes of the behavioral abnormalities are
different? The abstract should correct the conclusions in the body of the text.
Please review this paper.
user
Title: Behavioral abnormalities in C57BL/6 mice with chronic ulcerative colitis induced by DSS
Version: 1 Date: 15 Jan 2023
Reviewer's report:
We often encounter patients with ulcerative colitis who are mentally stressed or prone to
mental disorders. It is very interesting that the author reported the association between intestinal
inflammation, behavioral abnormalities, and the hippocampus using an experimental animal
model mouse.
I have a major or several minor questions with the report that the author has presented.
1) Should the authors do an objective scoring of enteritis and hippocampal pathology. Frankly,
I do not see any difference between the CONTROL and DSS groups. To substantiate this, an
objective score should be provided.
At the risk of repeating myself, the author notes in his discussion that the hippocampal pathology
in the 3% DSS group was not significantly different versus the control group. How does the
author conclude that there was no significant difference, something that is not quantified?
2) Does the unpaired t test (two-tailed) mentioned in the statistical methods section mean the
same thing as the T test (and nonparametric tests)? Would it be different? I suggest you correct
it, as it is difficult for the reader to understand. Or please check with a statistician.
3) (Fig 3 e) If you found a significant difference in sucrose water consumption, the author should
show it on the graph. Was there a significant decrease in sucrose water drinking? Or was there
no significant difference in it?
4) In the conclusion, the author states that both the acute and chronic enteritis models
cause behavioral abnormalities. In the acute enteritis model, enteritis symptoms cause
behavioral abnormalities. In the chronic enteritis model, psychological stress causes behavioral
abnormalities. However, based on the results presented by the authors, can we say that in the
chronic enteritis model, enteritis symptoms are not the cause?
5) The sucrose preference test; what does it mean that the acute enteritis model has reduced
preference and the chronic enteritis model does not? The authors should discuss that in the
discussion.
6) In the discussion, "Therefore, we speculated whether the mice in the DSS-induced UC model
had behavioral abnormalities, and hoped to improve the However, there is no experimental
system (whether or not enteritis is improved) in which a therapeutic drug (antipsychotic) was
used on mice in the DSS-induced UC model in this study. There is a disconnect between the
objectives and conclusions.
7) What the author states in the conclusion of the abstract is different from what is presented
in the results. Didn't both models, acute and chronic enteritis models, cause behavioral
abnormalities? Don't you want to state that the causes of the behavioral abnormalities are
different? The abstract should correct the conclusions in the body of the text.
assistant