Title: The contribution of lysophosphatidic acid receptors in the response of human lower
esophageal sphincter under the electrical field stimulation
Version: 1 Date: 20 Jan 2023
Reviewer's report:
Feng et al. present an experimental  study with ex-vivo fragments of human esophagi to evaluate
the role of lysophosphatidic acid receptors  in the regulation of the lower esophageal sphincter
motility.
The idea is interesting and it is a follow up of previous experiemnts by the group.  The
manuscript; however, needs some clarification and perhaps another set of experiments to
adequate the methodology.
1) why this is called a "randomized comtrolled  study"?
2) I am familiar with the methodology but most readers will not be. A figure of the organ bath
would be contributory
3) Figures presented are not  comprehensible per se.  They represent a single result and not the
average for the n=28 as I understood. Tables would be more adequate.
4) In regards to the methodology, 2 points are lacking. First, how the specimens were preserved
until the experiment? How long between the operation retrieval and  the experiment? If this
was variable, vitality of the samples could be compromized and the second and most important
criticism is that there is no control group. How do I know that there was no effect of the frug or
that the muscle was simpling dying or weaking with time?
Title: The contribution of lysophosphatidic acid receptors in the response of human lower
esophageal sphincter under the electrical field stimulation
Version: 1 Date: 20 Jan 2023
Reviewer's report:
Feng et al. present an experimental  study with ex-vivo fragments of human esophagi to evaluate
the role of lysophosphatidic acid receptors  in the regulation of the lower esophageal sphincter
motility.
The idea is interesting and it is a follow up of previous experiemnts by the group.  The
manuscript; however, needs some clarification and perhaps another set of experiments to
adequate the methodology.
1) why this is called a "randomized comtrolled  study"?
2) I am familiar with the methodology but most readers will not be. A figure of the organ bath
would be contributory
3) Figures presented are not  comprehensible per se.  They represent a single result and not the
average for the n=28 as I understood. Tables would be more adequate.
4) In regards to the methodology, 2 points are lacking. First, how the specimens were preserved
until the experiment? How long between the operation retrieval and  the experiment? If this
was variable, vitality of the samples could be compromized and the second and most important
criticism is that there is no control group. How do I know that there was no effect of the frug or
that the muscle was simpling dying or weaking with time?
Please review this paper.
user
Title: The contribution of lysophosphatidic acid receptors in the response of human lower
esophageal sphincter under the electrical field stimulation
Version: 1 Date: 20 Jan 2023
Reviewer's report:
Feng et al. present an experimental  study with ex-vivo fragments of human esophagi to evaluate
the role of lysophosphatidic acid receptors  in the regulation of the lower esophageal sphincter
motility.
The idea is interesting and it is a follow up of previous experiemnts by the group.  The
manuscript; however, needs some clarification and perhaps another set of experiments to
adequate the methodology.
1) why this is called a "randomized comtrolled  study"?
2) I am familiar with the methodology but most readers will not be. A figure of the organ bath
would be contributory
3) Figures presented are not  comprehensible per se.  They represent a single result and not the
average for the n=28 as I understood. Tables would be more adequate.
4) In regards to the methodology, 2 points are lacking. First, how the specimens were preserved
until the experiment? How long between the operation retrieval and  the experiment? If this
was variable, vitality of the samples could be compromized and the second and most important
criticism is that there is no control group. How do I know that there was no effect of the frug or
that the muscle was simpling dying or weaking with time?
assistant