Title: Discriminant equation using mucosally expressed cytokines and transcription factor for
making definite diagnosis of inflammatory bowel disease unclassified
Version: 1 Date: 16 Dec 2020
Reviewer's report:
This manuscript examined whether the discriminant equation using the mucosally expressed
mediators could characterize IBD-U, PSC-UC, or AIP-UC. The authors found that the
discriminant equation using IL-13, IL-21 and T-bet can be used as a diagnostic tool for IBD-U,
PSC-UC and AIP-UC. The findings are interesting, but I have some comments.
My comments are as follows. 1) Was there a difference in the mucosal expression of
inflammatory mediators with age? Also, does the use of drugs, especially anti-TNF# antibody,
affect the expression of inflammatory mediators? 2) Authors examined whether there was any
difference in the expression patterns of inflammatory mediators based on the different locations
in one patient each with UC or CD. Then authors showed there are similar expression patterns
of inflammatory mediators among them. Are all these samples taken from inflamed areas? This
is because the ileum is less prone to inflammation in patients with ulcerative colitis. 3) Authors
showed that the three-parameter discriminant equation diagnosed patients with IBD-U as UC
or CD correctly with high accuracy. Please describe the clinical importance of this result more
concretely in the discussion section, for example, possibility of early intervention with biologic
agents before definitive diagnosis. 4) Please explain the principal component analysis in a little
more detail.
I hope these comments will be helpful.
Title: Discriminant equation using mucosally expressed cytokines and transcription factor for
making definite diagnosis of inflammatory bowel disease unclassified
Version: 1 Date: 16 Dec 2020
Reviewer's report:
This manuscript examined whether the discriminant equation using the mucosally expressed
mediators could characterize IBD-U, PSC-UC, or AIP-UC. The authors found that the
discriminant equation using IL-13, IL-21 and T-bet can be used as a diagnostic tool for IBD-U,
PSC-UC and AIP-UC. The findings are interesting, but I have some comments.
My comments are as follows. 1) Was there a difference in the mucosal expression of
inflammatory mediators with age? Also, does the use of drugs, especially anti-TNF# antibody,
affect the expression of inflammatory mediators? 2) Authors examined whether there was any
difference in the expression patterns of inflammatory mediators based on the different locations
in one patient each with UC or CD. Then authors showed there are similar expression patterns
of inflammatory mediators among them. Are all these samples taken from inflamed areas? This
is because the ileum is less prone to inflammation in patients with ulcerative colitis. 3) Authors
showed that the three-parameter discriminant equation diagnosed patients with IBD-U as UC
or CD correctly with high accuracy. Please describe the clinical importance of this result more
concretely in the discussion section, for example, possibility of early intervention with biologic
agents before definitive diagnosis. 4) Please explain the principal component analysis in a little
more detail.
I hope these comments will be helpful.
Please review this paper.
user
Title: Discriminant equation using mucosally expressed cytokines and transcription factor for
making definite diagnosis of inflammatory bowel disease unclassified
Version: 1 Date: 16 Dec 2020
Reviewer's report:
This manuscript examined whether the discriminant equation using the mucosally expressed
mediators could characterize IBD-U, PSC-UC, or AIP-UC. The authors found that the
discriminant equation using IL-13, IL-21 and T-bet can be used as a diagnostic tool for IBD-U,
PSC-UC and AIP-UC. The findings are interesting, but I have some comments.
My comments are as follows. 1) Was there a difference in the mucosal expression of
inflammatory mediators with age? Also, does the use of drugs, especially anti-TNF# antibody,
affect the expression of inflammatory mediators? 2) Authors examined whether there was any
difference in the expression patterns of inflammatory mediators based on the different locations
in one patient each with UC or CD. Then authors showed there are similar expression patterns
of inflammatory mediators among them. Are all these samples taken from inflamed areas? This
is because the ileum is less prone to inflammation in patients with ulcerative colitis. 3) Authors
showed that the three-parameter discriminant equation diagnosed patients with IBD-U as UC
or CD correctly with high accuracy. Please describe the clinical importance of this result more
concretely in the discussion section, for example, possibility of early intervention with biologic
agents before definitive diagnosis. 4) Please explain the principal component analysis in a little
more detail.
I hope these comments will be helpful.
assistant