Title: SPATS2 is correlated with cell cycle progression and immune cells infiltration in
hepatocellular carcinoma
Version: 2 Date: 19 Oct 2022
Reviewer's report:
In this study the authros assessed the spermatogenesis associated serine rich 2 (SPATS2)
abnormal expression is linked in HCC. They found that the elevated expression of SPATS2
was unfavorably associated with the clinical pathological stage and prognosis. SPATS2 resulted
to be associated with cell cycle, apoptosis and cancer cell metastasis processes in HCC. Our
results confirmed that knockdown of SPATS2 will affect cell cycle, apoptosis and invasion of
HCC cell lines. In addition, SPATS2 expression was positively correlated with immune cell
or gene markers of immune infiltration in HCC. They concluded that SPATS2 is associated
with progression and immune infiltration, and could serve as a prognostic biomarker for HCC
suggesting the potential of SPATS2 to be used as a therapeutic target for HCC.
The immune cell and immune microenvironment of HCC is known to be characterized by
immunosuppressive cell populations. In particular, CD4+CD25 FOXp3 regulatory T cells are
well known as the major immunosuppressive cell population in HCC. However, the author do
not describe in detail immune cell and immune infiltration (they only report "B cells, CD4+
T cells, Macrophage, Neutrophil, and Dendritic cells"). This is an important issue as current
immunotherapies are agents acting to reduce the cancer-induced suppression of anti-tumor
immune response.
Title: SPATS2 is correlated with cell cycle progression and immune cells infiltration in
hepatocellular carcinoma
Version: 2 Date: 19 Oct 2022
Reviewer's report:
In this study the authros assessed the spermatogenesis associated serine rich 2 (SPATS2)
abnormal expression is linked in HCC. They found that the elevated expression of SPATS2
was unfavorably associated with the clinical pathological stage and prognosis. SPATS2 resulted
to be associated with cell cycle, apoptosis and cancer cell metastasis processes in HCC. Our
results confirmed that knockdown of SPATS2 will affect cell cycle, apoptosis and invasion of
HCC cell lines. In addition, SPATS2 expression was positively correlated with immune cell
or gene markers of immune infiltration in HCC. They concluded that SPATS2 is associated
with progression and immune infiltration, and could serve as a prognostic biomarker for HCC
suggesting the potential of SPATS2 to be used as a therapeutic target for HCC.
The immune cell and immune microenvironment of HCC is known to be characterized by
immunosuppressive cell populations. In particular, CD4+CD25 FOXp3 regulatory T cells are
well known as the major immunosuppressive cell population in HCC. However, the author do
not describe in detail immune cell and immune infiltration (they only report "B cells, CD4+
T cells, Macrophage, Neutrophil, and Dendritic cells"). This is an important issue as current
immunotherapies are agents acting to reduce the cancer-induced suppression of anti-tumor
immune response.
Please review this paper.
user
Title: SPATS2 is correlated with cell cycle progression and immune cells infiltration in
hepatocellular carcinoma
Version: 2 Date: 19 Oct 2022
Reviewer's report:
In this study the authros assessed the spermatogenesis associated serine rich 2 (SPATS2)
abnormal expression is linked in HCC. They found that the elevated expression of SPATS2
was unfavorably associated with the clinical pathological stage and prognosis. SPATS2 resulted
to be associated with cell cycle, apoptosis and cancer cell metastasis processes in HCC. Our
results confirmed that knockdown of SPATS2 will affect cell cycle, apoptosis and invasion of
HCC cell lines. In addition, SPATS2 expression was positively correlated with immune cell
or gene markers of immune infiltration in HCC. They concluded that SPATS2 is associated
with progression and immune infiltration, and could serve as a prognostic biomarker for HCC
suggesting the potential of SPATS2 to be used as a therapeutic target for HCC.
The immune cell and immune microenvironment of HCC is known to be characterized by
immunosuppressive cell populations. In particular, CD4+CD25 FOXp3 regulatory T cells are
well known as the major immunosuppressive cell population in HCC. However, the author do
not describe in detail immune cell and immune infiltration (they only report "B cells, CD4+
T cells, Macrophage, Neutrophil, and Dendritic cells"). This is an important issue as current
immunotherapies are agents acting to reduce the cancer-induced suppression of anti-tumor
immune response.
assistant