Reviewer's report
Title:Stromal expression of miR-21 in T3-4a colorectal cancer is an independent
predictor of early tumor relapse
Version:1Date:31 July 2014
Reviewer: manuela gariboldi
Reviewer's report:
Authors investigate miR-21 expression in advanced CRC and evaluated its
correlation with markers of progression in 277 T3/T4a patients. The question
posed is well defined. As stated in the introduction, it is already known that
expression of miR-21 is directly correlated with tumor progression, poor survival
and response to chemotherapy in CRC. Also predominant expression of this
miRNA in the tumor surrounding tissue has been already shown. Their results
obtained by ISH of miR-21 on TMAs support what already found. Correlation of
miR-21 levels with clinical variables confirms its higher expression in advanced
cancers. In addition, authors found that miR-21 expression is inversely correlated
with that of E-cadherin but directly related with that of MTA1. Interestingly miR-21
expression was linked to shorter disease free survival only for colon and not for
rectal cancer. Finally, its expression was lower in stage II cases with shorter
RFS. This result was not confirmed in Stage III cases.
Methods are appropriate and well described.
Major Compulsory Revisions
Authors should better discuss the differences in RFS observed between stage II
and stage III cases and among colon and rectal cancers.
Minor Essential Revisions
figure 1B: there is a typo error: insert and not inset
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Stromal expression of miR-21 in T3-4a colorectal cancer is an independent
predictor of early tumor relapse
Version:1Date:31 July 2014
Reviewer: manuela gariboldi
Reviewer's report:
Authors investigate miR-21 expression in advanced CRC and evaluated its
correlation with markers of progression in 277 T3/T4a patients. The question
posed is well defined. As stated in the introduction, it is already known that
expression of miR-21 is directly correlated with tumor progression, poor survival
and response to chemotherapy in CRC. Also predominant expression of this
miRNA in the tumor surrounding tissue has been already shown. Their results
obtained by ISH of miR-21 on TMAs support what already found. Correlation of
miR-21 levels with clinical variables confirms its higher expression in advanced
cancers. In addition, authors found that miR-21 expression is inversely correlated
with that of E-cadherin but directly related with that of MTA1. Interestingly miR-21
expression was linked to shorter disease free survival only for colon and not for
rectal cancer. Finally, its expression was lower in stage II cases with shorter
RFS. This result was not confirmed in Stage III cases.
Methods are appropriate and well described.
Major Compulsory Revisions
Authors should better discuss the differences in RFS observed between stage II
and stage III cases and among colon and rectal cancers.
Minor Essential Revisions
figure 1B: there is a typo error: insert and not inset
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Stromal expression of miR-21 in T3-4a colorectal cancer is an independent
predictor of early tumor relapse
Version:1Date:31 July 2014
Reviewer: manuela gariboldi
Reviewer's report:
Authors investigate miR-21 expression in advanced CRC and evaluated its
correlation with markers of progression in 277 T3/T4a patients. The question
posed is well defined. As stated in the introduction, it is already known that
expression of miR-21 is directly correlated with tumor progression, poor survival
and response to chemotherapy in CRC. Also predominant expression of this
miRNA in the tumor surrounding tissue has been already shown. Their results
obtained by ISH of miR-21 on TMAs support what already found. Correlation of
miR-21 levels with clinical variables confirms its higher expression in advanced
cancers. In addition, authors found that miR-21 expression is inversely correlated
with that of E-cadherin but directly related with that of MTA1. Interestingly miR-21
expression was linked to shorter disease free survival only for colon and not for
rectal cancer. Finally, its expression was lower in stage II cases with shorter
RFS. This result was not confirmed in Stage III cases.
Methods are appropriate and well described.
Major Compulsory Revisions
Authors should better discuss the differences in RFS observed between stage II
and stage III cases and among colon and rectal cancers.
Minor Essential Revisions
figure 1B: there is a typo error: insert and not inset
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I declare that I have no competing interests
assistant