Title: Efficacy of extracorporeal immunoadsorption therapy for removal of HBsAg
Version: 2 Date: 18 Mar 2025
Reviewer's report:
Hi!
Interesting article in terms of preparation medical device but some information still unclear:
1.  Antibody structure used for affinity media preparation was not discussed.
2.  Effectiveness of  ligand immobilization on the matrix was studied only trough microscope
analysis.  Passivation of activated  -CHO groups was not discussed.
3. "Adsorption",  "Adsorption efficiency",  "Adsorption ratio", "Adsorption rate" used for same
parameter but there no definition of it in the article.
4. some  topic titles have to be reconsidered.
5.  Need to clarify  when something is "adsorbed" by  media.
6. Dynamic and static adsorption section can be reviewed to provide better understanding of
these experiments.
7. "Immunosorbent adsorption percentage " shall be better defined and difference between it
and "efficacy" should be shown.
8. Difference in numbers between in vivo and in vitro experiments can be discussed.
9. ability to reduce DNA concentration shall be discussed.
I include manuscript with some comments.  I ask  editor to decide are my comments valuable or
not and to decide  can this article  to be published or not. As a specialist i enjoyed reading and
want to see the results of  this work to be published but some items can be more clarified.
Best wishes and good luck with your work!
Title: Efficacy of extracorporeal immunoadsorption therapy for removal of HBsAg
Version: 2 Date: 18 Mar 2025
Reviewer's report:
Hi!
Interesting article in terms of preparation medical device but some information still unclear:
1.  Antibody structure used for affinity media preparation was not discussed.
2.  Effectiveness of  ligand immobilization on the matrix was studied only trough microscope
analysis.  Passivation of activated  -CHO groups was not discussed.
3. "Adsorption",  "Adsorption efficiency",  "Adsorption ratio", "Adsorption rate" used for same
parameter but there no definition of it in the article.
4. some  topic titles have to be reconsidered.
5.  Need to clarify  when something is "adsorbed" by  media.
6. Dynamic and static adsorption section can be reviewed to provide better understanding of
these experiments.
7. "Immunosorbent adsorption percentage " shall be better defined and difference between it
and "efficacy" should be shown.
8. Difference in numbers between in vivo and in vitro experiments can be discussed.
9. ability to reduce DNA concentration shall be discussed.
I include manuscript with some comments.  I ask  editor to decide are my comments valuable or
not and to decide  can this article  to be published or not. As a specialist i enjoyed reading and
want to see the results of  this work to be published but some items can be more clarified.
Best wishes and good luck with your work!
Please review this paper.
user
Title: Efficacy of extracorporeal immunoadsorption therapy for removal of HBsAg
Version: 2 Date: 18 Mar 2025
Reviewer's report:
Hi!
Interesting article in terms of preparation medical device but some information still unclear:
1.  Antibody structure used for affinity media preparation was not discussed.
2.  Effectiveness of  ligand immobilization on the matrix was studied only trough microscope
analysis.  Passivation of activated  -CHO groups was not discussed.
3. "Adsorption",  "Adsorption efficiency",  "Adsorption ratio", "Adsorption rate" used for same
parameter but there no definition of it in the article.
4. some  topic titles have to be reconsidered.
5.  Need to clarify  when something is "adsorbed" by  media.
6. Dynamic and static adsorption section can be reviewed to provide better understanding of
these experiments.
7. "Immunosorbent adsorption percentage " shall be better defined and difference between it
and "efficacy" should be shown.
8. Difference in numbers between in vivo and in vitro experiments can be discussed.
9. ability to reduce DNA concentration shall be discussed.
I include manuscript with some comments.  I ask  editor to decide are my comments valuable or
not and to decide  can this article  to be published or not. As a specialist i enjoyed reading and
want to see the results of  this work to be published but some items can be more clarified.
Best wishes and good luck with your work!
assistant