Reviewer's report
Title:The MLH1 2101C>A (Q701K) variation is related to risk of gastric cancer in
Chinese males
Version: 1Date:23 November 2010
Reviewer: TL Chan
Reviewer's report:
Current manuscript described the mutation spectrum of MLH1 in the cohort of
Chinese patients developed gastric cancer. The authors identified six mutations
including 2 in the promoter region and 4 in the coding region. However, none of
them shows direct evidence of pathogenic mutation. The association of MLH1
2101C>A and gastric cancer has been proposed although the authors did not
provide explanation for MLH1 2101 C>A that only affect a specific age group.
Specific comments:
1) The authors did not state clearly why MLH1 gene is selected and examined.
Even MLH1 was selected, it is not clear immunohistochemistry/MSI test, which
are effective screening step in colorectal cancer associated with MMR genes
defect, was not adopted as a preliminary screening strategy.
2) The background of MLH1 methylation should be addressed and it is, in fact,
one of the publication of Yap.
3) The correlation of mutation and family history of gastric cancer of the patient
should be provided.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:The MLH1 2101C>A (Q701K) variation is related to risk of gastric cancer in
Chinese males
Version: 1Date:23 November 2010
Reviewer: TL Chan
Reviewer's report:
Current manuscript described the mutation spectrum of MLH1 in the cohort of
Chinese patients developed gastric cancer. The authors identified six mutations
including 2 in the promoter region and 4 in the coding region. However, none of
them shows direct evidence of pathogenic mutation. The association of MLH1
2101C>A and gastric cancer has been proposed although the authors did not
provide explanation for MLH1 2101 C>A that only affect a specific age group.
Specific comments:
1) The authors did not state clearly why MLH1 gene is selected and examined.
Even MLH1 was selected, it is not clear immunohistochemistry/MSI test, which
are effective screening step in colorectal cancer associated with MMR genes
defect, was not adopted as a preliminary screening strategy.
2) The background of MLH1 methylation should be addressed and it is, in fact,
one of the publication of Yap.
3) The correlation of mutation and family history of gastric cancer of the patient
should be provided.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:The MLH1 2101C>A (Q701K) variation is related to risk of gastric cancer in
Chinese males
Version: 1Date:23 November 2010
Reviewer: TL Chan
Reviewer's report:
Current manuscript described the mutation spectrum of MLH1 in the cohort of
Chinese patients developed gastric cancer. The authors identified six mutations
including 2 in the promoter region and 4 in the coding region. However, none of
them shows direct evidence of pathogenic mutation. The association of MLH1
2101C>A and gastric cancer has been proposed although the authors did not
provide explanation for MLH1 2101 C>A that only affect a specific age group.
Specific comments:
1) The authors did not state clearly why MLH1 gene is selected and examined.
Even MLH1 was selected, it is not clear immunohistochemistry/MSI test, which
are effective screening step in colorectal cancer associated with MMR genes
defect, was not adopted as a preliminary screening strategy.
2) The background of MLH1 methylation should be addressed and it is, in fact,
one of the publication of Yap.
3) The correlation of mutation and family history of gastric cancer of the patient
should be provided.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
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