Title: CircRNA circ-ATAD1 suppresses miR-618 maturation to participate in colorectal cancer
Version: 1 Date: 06 May 2021
Reviewer's report:
Cao and colleges examined the relationship between circ-ATAD1 and miR-168 in colorectal
cancer. Although the data shown by the authors are interesting, the paper lacks context thus
difficult to understand why authors conducted some of the experiments. In particular, the
methods in which authors identified the key target miRNA (miR-168) is unclear, but this is
perhaps the most novel part of the study. Therefore, there is a need for the authors to explain
in detail, how and why miR-168 was selected as the potential target. In addition, authors
should consider conducting additional functional experiments to show what is the cause of the
increasing proliferation of circ-ATAD1.
Please add, a target identification data to the manuscript explaining, how authors identified
miR-168 as the target genes for circRNA.
Please use actual values for qRT PCR data and represent the data as box and whisker plot or
something that is easier to evaluate the differences in gene expression (rather than heap map).
Also please conduct statistical analysis to show that the gene expression is different.
Title: CircRNA circ-ATAD1 suppresses miR-618 maturation to participate in colorectal cancer
Version: 1 Date: 06 May 2021
Reviewer's report:
Cao and colleges examined the relationship between circ-ATAD1 and miR-168 in colorectal
cancer. Although the data shown by the authors are interesting, the paper lacks context thus
difficult to understand why authors conducted some of the experiments. In particular, the
methods in which authors identified the key target miRNA (miR-168) is unclear, but this is
perhaps the most novel part of the study. Therefore, there is a need for the authors to explain
in detail, how and why miR-168 was selected as the potential target. In addition, authors
should consider conducting additional functional experiments to show what is the cause of the
increasing proliferation of circ-ATAD1.
Please add, a target identification data to the manuscript explaining, how authors identified
miR-168 as the target genes for circRNA.
Please use actual values for qRT PCR data and represent the data as box and whisker plot or
something that is easier to evaluate the differences in gene expression (rather than heap map).
Also please conduct statistical analysis to show that the gene expression is different.
Please review this paper.
user
Title: CircRNA circ-ATAD1 suppresses miR-618 maturation to participate in colorectal cancer
Version: 1 Date: 06 May 2021
Reviewer's report:
Cao and colleges examined the relationship between circ-ATAD1 and miR-168 in colorectal
cancer. Although the data shown by the authors are interesting, the paper lacks context thus
difficult to understand why authors conducted some of the experiments. In particular, the
methods in which authors identified the key target miRNA (miR-168) is unclear, but this is
perhaps the most novel part of the study. Therefore, there is a need for the authors to explain
in detail, how and why miR-168 was selected as the potential target. In addition, authors
should consider conducting additional functional experiments to show what is the cause of the
increasing proliferation of circ-ATAD1.
Please add, a target identification data to the manuscript explaining, how authors identified
miR-168 as the target genes for circRNA.
Please use actual values for qRT PCR data and represent the data as box and whisker plot or
something that is easier to evaluate the differences in gene expression (rather than heap map).
Also please conduct statistical analysis to show that the gene expression is different.
assistant