Title: PVA enema ameliorates DSS-induced acute colitis in mice
Version: 1 Date: 16 Aug 2023
Reviewer:  Sumeet patiyal patiyal
Reviewer's report:
The authors have presented a very interesting and novel approach. They have  observed that
administering an aqueous solution of Polyvinyl alcohol at concentrations of 1 mg/ml and 3 mg/
ml led to a noteworthy reduction in weight loss among the colitis-affected group. Additionally,
there was a substantial enhancement in histopathology scores, along with a significant decrease
in the levels of inflammatory factors within the intestinal mucosal tissue. Furthermore, it was
established that PVA had the capacity to significantly amplify the count of colonic organoids in
an in vitro setting. However, I have the following comments:
1) "Furthermore, many children also suffer from this disease. This has seriously affected the
growth and development of children". This sentences seems like out of context, I would suggest
authors to provide a statistics along with relevant references.
2) Sentences in the Introduction seems incomplete at many places. Please check and rectify the
same.
3) Second paragraph of Introduction needs rearranging the sentences as the flow of sentences
is terrible.
4) Rewrite the following sentence:
"Nevertheless, there are also many and serious complications after the operation".
5) Please provide the full-form of SEM, as the audience could not be expert in statistics.
6) What is the rationale behind using the parametric test as ANOVA? Has authors checked the
distribution of their data.
7) I would suggest authors to provide more recent evidences support their claims.
8) A strong conclusion is recommended.
9) There are many grammatical and punctuation errors. Please lookout for them in the manuscript
and rectify them.
10) I I would suggest all the authors to read the manuscript thoroughly and make the flow
readable, as the present form of the manuscript is not up to the mark.
11) I failed to understand the motivation behind choosing the PVA. Kindly provide the same
in the introduction.
12) Have authors considered other bio materials too before choosing the PVA?
Title: PVA enema ameliorates DSS-induced acute colitis in mice
Version: 1 Date: 16 Aug 2023
Reviewer:  Sumeet patiyal patiyal
Reviewer's report:
The authors have presented a very interesting and novel approach. They have  observed that
administering an aqueous solution of Polyvinyl alcohol at concentrations of 1 mg/ml and 3 mg/
ml led to a noteworthy reduction in weight loss among the colitis-affected group. Additionally,
there was a substantial enhancement in histopathology scores, along with a significant decrease
in the levels of inflammatory factors within the intestinal mucosal tissue. Furthermore, it was
established that PVA had the capacity to significantly amplify the count of colonic organoids in
an in vitro setting. However, I have the following comments:
1) "Furthermore, many children also suffer from this disease. This has seriously affected the
growth and development of children". This sentences seems like out of context, I would suggest
authors to provide a statistics along with relevant references.
2) Sentences in the Introduction seems incomplete at many places. Please check and rectify the
same.
3) Second paragraph of Introduction needs rearranging the sentences as the flow of sentences
is terrible.
4) Rewrite the following sentence:
"Nevertheless, there are also many and serious complications after the operation".
5) Please provide the full-form of SEM, as the audience could not be expert in statistics.
6) What is the rationale behind using the parametric test as ANOVA? Has authors checked the
distribution of their data.
7) I would suggest authors to provide more recent evidences support their claims.
8) A strong conclusion is recommended.
9) There are many grammatical and punctuation errors. Please lookout for them in the manuscript
and rectify them.
10) I I would suggest all the authors to read the manuscript thoroughly and make the flow
readable, as the present form of the manuscript is not up to the mark.
11) I failed to understand the motivation behind choosing the PVA. Kindly provide the same
in the introduction.
12) Have authors considered other bio materials too before choosing the PVA?
Please review this paper.
user
Title: PVA enema ameliorates DSS-induced acute colitis in mice
Version: 1 Date: 16 Aug 2023
Reviewer:  Sumeet patiyal patiyal
Reviewer's report:
The authors have presented a very interesting and novel approach. They have  observed that
administering an aqueous solution of Polyvinyl alcohol at concentrations of 1 mg/ml and 3 mg/
ml led to a noteworthy reduction in weight loss among the colitis-affected group. Additionally,
there was a substantial enhancement in histopathology scores, along with a significant decrease
in the levels of inflammatory factors within the intestinal mucosal tissue. Furthermore, it was
established that PVA had the capacity to significantly amplify the count of colonic organoids in
an in vitro setting. However, I have the following comments:
1) "Furthermore, many children also suffer from this disease. This has seriously affected the
growth and development of children". This sentences seems like out of context, I would suggest
authors to provide a statistics along with relevant references.
2) Sentences in the Introduction seems incomplete at many places. Please check and rectify the
same.
3) Second paragraph of Introduction needs rearranging the sentences as the flow of sentences
is terrible.
4) Rewrite the following sentence:
"Nevertheless, there are also many and serious complications after the operation".
5) Please provide the full-form of SEM, as the audience could not be expert in statistics.
6) What is the rationale behind using the parametric test as ANOVA? Has authors checked the
distribution of their data.
7) I would suggest authors to provide more recent evidences support their claims.
8) A strong conclusion is recommended.
9) There are many grammatical and punctuation errors. Please lookout for them in the manuscript
and rectify them.
10) I I would suggest all the authors to read the manuscript thoroughly and make the flow
readable, as the present form of the manuscript is not up to the mark.
11) I failed to understand the motivation behind choosing the PVA. Kindly provide the same
in the introduction.
12) Have authors considered other bio materials too before choosing the PVA?
assistant