Title: Bile acid diarrhoea and metabolic changes after cholecystectomy: A prospective case-
control study
Version: 1 Date: 21 Jun 2024
Reviewer's report:
The authors have conducted this study to determine the incidence of bile acid diarrhea and the
factors associated with it. I have following comments regarding the manuscript:
1. The title does not reflect the content of the manuscript. Please revise it as "Bile acid diarrhea
and metabolic changes after cholecystectomy: A prospective case-control study:
2. It is mentioned in the Results of the Abstract that "Gallbladder PPAR # was found to have
a significant correlation with stool consistency, with the lower the PPAR# concentration the
higher the Bristol stool chart number (i.e. looser stool). " Please mention its significance in the
Conclusion.
3. In the Abstract conclusion it is mentioned that "There was also no correlation with PPAR#
suggesting the observed rise is independent of this pathway. " Please add a sentence in Results
section to support this sentence.
4. In the Methods section, Please mention the following details:
a. Type of study - Prospective or retrospective
b. Study period
c. Study center
d. How was sample size calculation done?
e. BSG criteria for diarrhea
5. Why were bariatric surgery patients admitted in the control group as it is a major surgery
which can significantly affect the lipid profile and bowel frequency of the patients and affect
the results?
6. How was gallbladder tissue sample tested for FGF19 and PPAR# in the study group patients?
Please mention its methodology.
7. Please mention the p values in the Table 1.
8.  Please mention the study limitations including small sample size, single center study, etc.
Title: Bile acid diarrhoea and metabolic changes after cholecystectomy: A prospective case-
control study
Version: 1 Date: 21 Jun 2024
Reviewer's report:
The authors have conducted this study to determine the incidence of bile acid diarrhea and the
factors associated with it. I have following comments regarding the manuscript:
1. The title does not reflect the content of the manuscript. Please revise it as "Bile acid diarrhea
and metabolic changes after cholecystectomy: A prospective case-control study:
2. It is mentioned in the Results of the Abstract that "Gallbladder PPAR # was found to have
a significant correlation with stool consistency, with the lower the PPAR# concentration the
higher the Bristol stool chart number (i.e. looser stool). " Please mention its significance in the
Conclusion.
3. In the Abstract conclusion it is mentioned that "There was also no correlation with PPAR#
suggesting the observed rise is independent of this pathway. " Please add a sentence in Results
section to support this sentence.
4. In the Methods section, Please mention the following details:
a. Type of study - Prospective or retrospective
b. Study period
c. Study center
d. How was sample size calculation done?
e. BSG criteria for diarrhea
5. Why were bariatric surgery patients admitted in the control group as it is a major surgery
which can significantly affect the lipid profile and bowel frequency of the patients and affect
the results?
6. How was gallbladder tissue sample tested for FGF19 and PPAR# in the study group patients?
Please mention its methodology.
7. Please mention the p values in the Table 1.
8.  Please mention the study limitations including small sample size, single center study, etc.
Please review this paper.
user
Title: Bile acid diarrhoea and metabolic changes after cholecystectomy: A prospective case-
control study
Version: 1 Date: 21 Jun 2024
Reviewer's report:
The authors have conducted this study to determine the incidence of bile acid diarrhea and the
factors associated with it. I have following comments regarding the manuscript:
1. The title does not reflect the content of the manuscript. Please revise it as "Bile acid diarrhea
and metabolic changes after cholecystectomy: A prospective case-control study:
2. It is mentioned in the Results of the Abstract that "Gallbladder PPAR # was found to have
a significant correlation with stool consistency, with the lower the PPAR# concentration the
higher the Bristol stool chart number (i.e. looser stool). " Please mention its significance in the
Conclusion.
3. In the Abstract conclusion it is mentioned that "There was also no correlation with PPAR#
suggesting the observed rise is independent of this pathway. " Please add a sentence in Results
section to support this sentence.
4. In the Methods section, Please mention the following details:
a. Type of study - Prospective or retrospective
b. Study period
c. Study center
d. How was sample size calculation done?
e. BSG criteria for diarrhea
5. Why were bariatric surgery patients admitted in the control group as it is a major surgery
which can significantly affect the lipid profile and bowel frequency of the patients and affect
the results?
6. How was gallbladder tissue sample tested for FGF19 and PPAR# in the study group patients?
Please mention its methodology.
7. Please mention the p values in the Table 1.
8.  Please mention the study limitations including small sample size, single center study, etc.
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