Title: The upregulation of stromal antigen 3 expression suppresses the phenotypic hallmarks
of hepatocellular carcinoma through the Smad3-CDK4/CDK6-cyclin D1 and CXCR4/RhoA
pathways
Version: 2 Date: 17 Mar 2022
Reviewer's report:
The manuscript by Zhao et al. reports the effect of STAG3 on hepatocellular carcinoma. Even
though the manuscript described an interesting story. However, I have some problems to improve
the manuscript, as follows:
1. In figure 1A, low expression of STAG3 in hepatocytes and high level of STAG3 in liver non-
parenchymal cells of both para-carcinoma tissues and cancer tissues.
2. The data of transfection efficiency (Figure 1F-G) should be put in Figure 2.
3. In this manuscript, STAG3 suppresses the proliferation, migration and invasion of hepatoma
cells. Meanwhile, STAG3 induces apoptosis of hepatoma cells. That is to say, the effect of
STAG3 on hepatoma cells is various. Why the authors investigate the mechanism about the cell
cycle-related pathway?
4. In figure 5A-B, the data of transfection efficiency should be added.
5. The localization of STAG3 is in the nuclei of liver non-parenchymal cells (Figure 1A). In
figure 2-5, the authors investigate the effect of STAG3 in HCC cell lines. The authors should
explain the mechanism of non-parenchymal cell-derived STAG3 affecting the proliferation,
migration, invasion, apoptosis of hepatocytes.
Title: The upregulation of stromal antigen 3 expression suppresses the phenotypic hallmarks
of hepatocellular carcinoma through the Smad3-CDK4/CDK6-cyclin D1 and CXCR4/RhoA
pathways
Version: 2 Date: 17 Mar 2022
Reviewer's report:
The manuscript by Zhao et al. reports the effect of STAG3 on hepatocellular carcinoma. Even
though the manuscript described an interesting story. However, I have some problems to improve
the manuscript, as follows:
1. In figure 1A, low expression of STAG3 in hepatocytes and high level of STAG3 in liver non-
parenchymal cells of both para-carcinoma tissues and cancer tissues.
2. The data of transfection efficiency (Figure 1F-G) should be put in Figure 2.
3. In this manuscript, STAG3 suppresses the proliferation, migration and invasion of hepatoma
cells. Meanwhile, STAG3 induces apoptosis of hepatoma cells. That is to say, the effect of
STAG3 on hepatoma cells is various. Why the authors investigate the mechanism about the cell
cycle-related pathway?
4. In figure 5A-B, the data of transfection efficiency should be added.
5. The localization of STAG3 is in the nuclei of liver non-parenchymal cells (Figure 1A). In
figure 2-5, the authors investigate the effect of STAG3 in HCC cell lines. The authors should
explain the mechanism of non-parenchymal cell-derived STAG3 affecting the proliferation,
migration, invasion, apoptosis of hepatocytes.
Please review this paper.
user
Title: The upregulation of stromal antigen 3 expression suppresses the phenotypic hallmarks
of hepatocellular carcinoma through the Smad3-CDK4/CDK6-cyclin D1 and CXCR4/RhoA
pathways
Version: 2 Date: 17 Mar 2022
Reviewer's report:
The manuscript by Zhao et al. reports the effect of STAG3 on hepatocellular carcinoma. Even
though the manuscript described an interesting story. However, I have some problems to improve
the manuscript, as follows:
1. In figure 1A, low expression of STAG3 in hepatocytes and high level of STAG3 in liver non-
parenchymal cells of both para-carcinoma tissues and cancer tissues.
2. The data of transfection efficiency (Figure 1F-G) should be put in Figure 2.
3. In this manuscript, STAG3 suppresses the proliferation, migration and invasion of hepatoma
cells. Meanwhile, STAG3 induces apoptosis of hepatoma cells. That is to say, the effect of
STAG3 on hepatoma cells is various. Why the authors investigate the mechanism about the cell
cycle-related pathway?
4. In figure 5A-B, the data of transfection efficiency should be added.
5. The localization of STAG3 is in the nuclei of liver non-parenchymal cells (Figure 1A). In
figure 2-5, the authors investigate the effect of STAG3 in HCC cell lines. The authors should
explain the mechanism of non-parenchymal cell-derived STAG3 affecting the proliferation,
migration, invasion, apoptosis of hepatocytes.
assistant