Title: PPAR# activation attenuates neonatal CRD-induced visceral pain sensitization and
anxiety in male rats by alleviating oxidative stress
Version: 4 Date: 23 Nov 2024
Reviewer's report:
Dear editor,
On the manuscript entitled: PPAR# activation attenuates neonatal CRD-induced visceral pain
sensitization and anxiety in male rats by alleviating oxidative stress
1- There are several spelling errors in the text that need to be corrected.
2- In the methods section of the abstract, it should be specified in which tissue SOD and MDA
were measured.
3- Sentence “In addition, rosiglitazone inhibited the upregulation of MDA and the
downregulation of SOD in the spinal cord” should be written better in the results section of
abstract.
4- In line 77, the number 2 should be deleted from the “Hasegawa2”.
5- Enough information regarding that CRD create symptoms of IBS should be added to the
introduction.
6- How many rats were tested?
7- Was the angioplasty balloon placed in the descending colon for only 60 seconds each day from
day 7 to 13? Is this enough time to induce IBS models? please insert appropriate references?
8- What was your rationale for choosing the dosage of the medications?
9- Why the GW9662 did not inject before the single doses of rosiglitazone? Did GW9662 inject
at single dose? please clarify.
10- The caption of the figure 1 is too long. It is better to remove the content that can be written
in the method section.
11- It is better to allocate a section in the Materials and methods section for grouping animals.
12- The caption of the figure 1A states that 20 neonatal rats were randomly divided into 2 groups
of 10 rats each: the Sham and the IBS group, but in figure 1B it says that 24 mice were divided
into 4 groups. Your writing style is confusing. Please write more clearly. Why different number
of rats were aligned to each group?
13- What was your rationale for choosing the pressures of 40 and 60 mmHg for
electromyography? I suggest that appropriate references putted for all methods.
14- Did you anesthetize the mice twice? Once for electromyography and once for SOD and
MDA measurements? Method of anesthesia and sacrificing need to be stated clearly.
15- In the results section on line 206, it would be better to remove SD from “SD rats”.
16- What is meant secondary CRD in line 207 in the phrase “On 50 d, secondary CRD was
performed on rats that had undergone neonatal CRD”? please clarify.
17- SOD, MDA and were only measured in animals that received repeated injections of
rosiglitazone, why were not measured in animals that received single dose of rosiglitazone?
18- Discussion is too long. Please also concise conclusion based on your core finding.
Title: PPAR# activation attenuates neonatal CRD-induced visceral pain sensitization and
anxiety in male rats by alleviating oxidative stress
Version: 4 Date: 23 Nov 2024
Reviewer's report:
Dear editor,
On the manuscript entitled: PPAR# activation attenuates neonatal CRD-induced visceral pain
sensitization and anxiety in male rats by alleviating oxidative stress
1- There are several spelling errors in the text that need to be corrected.
2- In the methods section of the abstract, it should be specified in which tissue SOD and MDA
were measured.
3- Sentence “In addition, rosiglitazone inhibited the upregulation of MDA and the
downregulation of SOD in the spinal cord” should be written better in the results section of
abstract.
4- In line 77, the number 2 should be deleted from the “Hasegawa2”.
5- Enough information regarding that CRD create symptoms of IBS should be added to the
introduction.
6- How many rats were tested?
7- Was the angioplasty balloon placed in the descending colon for only 60 seconds each day from
day 7 to 13? Is this enough time to induce IBS models? please insert appropriate references?
8- What was your rationale for choosing the dosage of the medications?
9- Why the GW9662 did not inject before the single doses of rosiglitazone? Did GW9662 inject
at single dose? please clarify.
10- The caption of the figure 1 is too long. It is better to remove the content that can be written
in the method section.
11- It is better to allocate a section in the Materials and methods section for grouping animals.
12- The caption of the figure 1A states that 20 neonatal rats were randomly divided into 2 groups
of 10 rats each: the Sham and the IBS group, but in figure 1B it says that 24 mice were divided
into 4 groups. Your writing style is confusing. Please write more clearly. Why different number
of rats were aligned to each group?
13- What was your rationale for choosing the pressures of 40 and 60 mmHg for
electromyography? I suggest that appropriate references putted for all methods.
14- Did you anesthetize the mice twice? Once for electromyography and once for SOD and
MDA measurements? Method of anesthesia and sacrificing need to be stated clearly.
15- In the results section on line 206, it would be better to remove SD from “SD rats”.
16- What is meant secondary CRD in line 207 in the phrase “On 50 d, secondary CRD was
performed on rats that had undergone neonatal CRD”? please clarify.
17- SOD, MDA and were only measured in animals that received repeated injections of
rosiglitazone, why were not measured in animals that received single dose of rosiglitazone?
18- Discussion is too long. Please also concise conclusion based on your core finding.
Please review this paper.
user
Title: PPAR# activation attenuates neonatal CRD-induced visceral pain sensitization and
anxiety in male rats by alleviating oxidative stress
Version: 4 Date: 23 Nov 2024
Reviewer's report:
Dear editor,
On the manuscript entitled: PPAR# activation attenuates neonatal CRD-induced visceral pain
sensitization and anxiety in male rats by alleviating oxidative stress
1- There are several spelling errors in the text that need to be corrected.
2- In the methods section of the abstract, it should be specified in which tissue SOD and MDA
were measured.
3- Sentence “In addition, rosiglitazone inhibited the upregulation of MDA and the
downregulation of SOD in the spinal cord” should be written better in the results section of
abstract.
4- In line 77, the number 2 should be deleted from the “Hasegawa2”.
5- Enough information regarding that CRD create symptoms of IBS should be added to the
introduction.
6- How many rats were tested?
7- Was the angioplasty balloon placed in the descending colon for only 60 seconds each day from
day 7 to 13? Is this enough time to induce IBS models? please insert appropriate references?
8- What was your rationale for choosing the dosage of the medications?
9- Why the GW9662 did not inject before the single doses of rosiglitazone? Did GW9662 inject
at single dose? please clarify.
10- The caption of the figure 1 is too long. It is better to remove the content that can be written
in the method section.
11- It is better to allocate a section in the Materials and methods section for grouping animals.
12- The caption of the figure 1A states that 20 neonatal rats were randomly divided into 2 groups
of 10 rats each: the Sham and the IBS group, but in figure 1B it says that 24 mice were divided
into 4 groups. Your writing style is confusing. Please write more clearly. Why different number
of rats were aligned to each group?
13- What was your rationale for choosing the pressures of 40 and 60 mmHg for
electromyography? I suggest that appropriate references putted for all methods.
14- Did you anesthetize the mice twice? Once for electromyography and once for SOD and
MDA measurements? Method of anesthesia and sacrificing need to be stated clearly.
15- In the results section on line 206, it would be better to remove SD from “SD rats”.
16- What is meant secondary CRD in line 207 in the phrase “On 50 d, secondary CRD was
performed on rats that had undergone neonatal CRD”? please clarify.
17- SOD, MDA and were only measured in animals that received repeated injections of
rosiglitazone, why were not measured in animals that received single dose of rosiglitazone?
18- Discussion is too long. Please also concise conclusion based on your core finding.
assistant