Reviewer's report
Title:Lubiprostone ameliorates the cystic fibrosis mouse intestinal phenotype
Version: 3Date:8 September 2010
Reviewer: Hugo De Jonge
Reviewer's report:
The author's reply to comments 1-3 of my re-review is satisfactory. With respect
to the author's answer to comment 1, it should be noted that ex vivo studies of
lubiprostone action in native mouse and human intestine, reporting a high
potency of lubiprostone in inducing CFTR activation via EP4-cAMP-PKA
signaling (EC50 of 50 nM; ref. 23), are clinically more relevant as compared to
studies in the A6 Xenopus kidney cell line, which might lack one ore more
components of this signal transduction pathway, such as EP4 receptors.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:Lubiprostone ameliorates the cystic fibrosis mouse intestinal phenotype
Version: 3Date:8 September 2010
Reviewer: Hugo De Jonge
Reviewer's report:
The author's reply to comments 1-3 of my re-review is satisfactory. With respect
to the author's answer to comment 1, it should be noted that ex vivo studies of
lubiprostone action in native mouse and human intestine, reporting a high
potency of lubiprostone in inducing CFTR activation via EP4-cAMP-PKA
signaling (EC50 of 50 nM; ref. 23), are clinically more relevant as compared to
studies in the A6 Xenopus kidney cell line, which might lack one ore more
components of this signal transduction pathway, such as EP4 receptors.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:Lubiprostone ameliorates the cystic fibrosis mouse intestinal phenotype
Version: 3Date:8 September 2010
Reviewer: Hugo De Jonge
Reviewer's report:
The author's reply to comments 1-3 of my re-review is satisfactory. With respect
to the author's answer to comment 1, it should be noted that ex vivo studies of
lubiprostone action in native mouse and human intestine, reporting a high
potency of lubiprostone in inducing CFTR activation via EP4-cAMP-PKA
signaling (EC50 of 50 nM; ref. 23), are clinically more relevant as compared to
studies in the A6 Xenopus kidney cell line, which might lack one ore more
components of this signal transduction pathway, such as EP4 receptors.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
assistant