Reviewer's report
Title:Ringer's Lactate improves liver recovery in a murine model of
acetaminophen toxicity
Version: 2Date:13 July 2011
Reviewer: Jim P. Luyendyk
Reviewer's report:
1) The authors should include the 48 hour data as a figure in the manuscript, and
described in the results section as opposed to the methods. This will be
beneficial to the reader as the primary conclusion that RLS improves recovery is
based on a single time point at which injury is nearly entirely resolved, based on
ALT activity.
2) The manuscript would benefit by the addition of another biomarker for liver
regeneration/hepatocyte proliferation, such as PCNA staining. The authors note
that they were unable to perform BrdU analysis on account of an animal protocol
issue. It is unclear why PCNA staining cannot be performed on existing paraffin
sections from the 24, 48, and 72 hour time points. Including these data would
substantially increase the impact of the manuscript. Perhaps there is increased
regeneration at 24 hours not reflected by ALT activity at that time point.
Minor comment: The new text (currently in methods) describing the 48 hour data
should read “did not show statistically lower serum…”
Level of interest: An article of limited interest
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
no competing interests.
Reviewer's report
Title:Ringer's Lactate improves liver recovery in a murine model of
acetaminophen toxicity
Version: 2Date:13 July 2011
Reviewer: Jim P. Luyendyk
Reviewer's report:
1) The authors should include the 48 hour data as a figure in the manuscript, and
described in the results section as opposed to the methods. This will be
beneficial to the reader as the primary conclusion that RLS improves recovery is
based on a single time point at which injury is nearly entirely resolved, based on
ALT activity.
2) The manuscript would benefit by the addition of another biomarker for liver
regeneration/hepatocyte proliferation, such as PCNA staining. The authors note
that they were unable to perform BrdU analysis on account of an animal protocol
issue. It is unclear why PCNA staining cannot be performed on existing paraffin
sections from the 24, 48, and 72 hour time points. Including these data would
substantially increase the impact of the manuscript. Perhaps there is increased
regeneration at 24 hours not reflected by ALT activity at that time point.
Minor comment: The new text (currently in methods) describing the 48 hour data
should read “did not show statistically lower serum…”
Level of interest: An article of limited interest
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
no competing interests.
Please review this paper.
user
Reviewer's report
Title:Ringer's Lactate improves liver recovery in a murine model of
acetaminophen toxicity
Version: 2Date:13 July 2011
Reviewer: Jim P. Luyendyk
Reviewer's report:
1) The authors should include the 48 hour data as a figure in the manuscript, and
described in the results section as opposed to the methods. This will be
beneficial to the reader as the primary conclusion that RLS improves recovery is
based on a single time point at which injury is nearly entirely resolved, based on
ALT activity.
2) The manuscript would benefit by the addition of another biomarker for liver
regeneration/hepatocyte proliferation, such as PCNA staining. The authors note
that they were unable to perform BrdU analysis on account of an animal protocol
issue. It is unclear why PCNA staining cannot be performed on existing paraffin
sections from the 24, 48, and 72 hour time points. Including these data would
substantially increase the impact of the manuscript. Perhaps there is increased
regeneration at 24 hours not reflected by ALT activity at that time point.
Minor comment: The new text (currently in methods) describing the 48 hour data
should read “did not show statistically lower serum…”
Level of interest: An article of limited interest
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
no competing interests.
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