Title: Unveiling gastric precancerous stages: metabolomic insights for early detection and
intervention
Version: 6 Date: 25 Mar 2025
Reviewer's report:
Sample Size & Generalizability:
"The single-center design and limited sample size (n=188) may affect the generalizability of
findings. Please discuss this limitation and consider multicentric validation in future studies."
Statistical Rigor:
"Were multiple testing corrections (e.g., FDR) applied for univariate analyses? Clarification is
needed to ensure robustness against Type I errors."
Mechanistic Insight:
"The in vitro validation focuses on gene expression changes. Additional experiments (e.g.,
functional assays) would strengthen the biological relevance of glycerophosphocholine/
tiglylcarnitine in delaying intestinalization."
Clinical Translation:
"Highlight how the proposed biomarkers (AUC 0.916) could integrate into existing diagnostic
workflows (e.g., endoscopy) to emphasize clinical utility."
Formatting & Typos:
"Correct 'Corea cascade' to 'Correa cascade' (Background section) and ensure figure legends are
free of placeholders ('Click here to Correct')."
Supplementary Data:
"Confirm that all referenced supplementary tables/figures (e.g., Supp. Table 4) are included and
properly labeled in the submission files."
Title: Unveiling gastric precancerous stages: metabolomic insights for early detection and
intervention
Version: 6 Date: 25 Mar 2025
Reviewer's report:
Sample Size & Generalizability:
"The single-center design and limited sample size (n=188) may affect the generalizability of
findings. Please discuss this limitation and consider multicentric validation in future studies."
Statistical Rigor:
"Were multiple testing corrections (e.g., FDR) applied for univariate analyses? Clarification is
needed to ensure robustness against Type I errors."
Mechanistic Insight:
"The in vitro validation focuses on gene expression changes. Additional experiments (e.g.,
functional assays) would strengthen the biological relevance of glycerophosphocholine/
tiglylcarnitine in delaying intestinalization."
Clinical Translation:
"Highlight how the proposed biomarkers (AUC 0.916) could integrate into existing diagnostic
workflows (e.g., endoscopy) to emphasize clinical utility."
Formatting & Typos:
"Correct 'Corea cascade' to 'Correa cascade' (Background section) and ensure figure legends are
free of placeholders ('Click here to Correct')."
Supplementary Data:
"Confirm that all referenced supplementary tables/figures (e.g., Supp. Table 4) are included and
properly labeled in the submission files."
Please review this paper.
user
Title: Unveiling gastric precancerous stages: metabolomic insights for early detection and
intervention
Version: 6 Date: 25 Mar 2025
Reviewer's report:
Sample Size & Generalizability:
"The single-center design and limited sample size (n=188) may affect the generalizability of
findings. Please discuss this limitation and consider multicentric validation in future studies."
Statistical Rigor:
"Were multiple testing corrections (e.g., FDR) applied for univariate analyses? Clarification is
needed to ensure robustness against Type I errors."
Mechanistic Insight:
"The in vitro validation focuses on gene expression changes. Additional experiments (e.g.,
functional assays) would strengthen the biological relevance of glycerophosphocholine/
tiglylcarnitine in delaying intestinalization."
Clinical Translation:
"Highlight how the proposed biomarkers (AUC 0.916) could integrate into existing diagnostic
workflows (e.g., endoscopy) to emphasize clinical utility."
Formatting & Typos:
"Correct 'Corea cascade' to 'Correa cascade' (Background section) and ensure figure legends are
free of placeholders ('Click here to Correct')."
Supplementary Data:
"Confirm that all referenced supplementary tables/figures (e.g., Supp. Table 4) are included and
properly labeled in the submission files."
assistant