Reviewer's report
Title:Constitutive activation of glycogen synthase kinase-3beta correlates with
better prognosis and cyclin-dependent kinase inhibitors in human gastric cancer
Version: 5Date:13 February 2010
Reviewer: Gregory Kouraklis
Reviewer's report:
The full length article by Yu Jin Cho et al describes the expression of pGSK-3b in
276 human gastric carcinoma speciments.
Other studies have studied the role of GSK-3b in gastric cancer cells.
Today, the role of GSK-3Bb in gastric cancer still remains inconclusive.
The authors of the current study found that GSK-3b activation was frequently
observed in early-stage gastric carcinoma.
Major Compulsory Revisions
1. In the section "Patients", it is written that "The files of 276 surgically resected
gatric cancer examined ....".
In the same section later it is written: "The cases enrolled in this study included
64 advanced and 217 early stage gastric carcinomas".
What is the total number, 276 or 281?
2. It is not stated whether surgical treatment was curative in all patients.
3. It should be stated whether patients received postoperative radio- or
chemotherapy. If additional treatment was given, this must be encounter for the
multivariate analysis.
Discretionary Revisions
1. The authors should state how the sections were assessed. What was the
biological justification (i.e. molecular genetic) for a 10% cutoff for scoring tumors
as positive or negative?
Overall, it would have been advantageous for the authors to have analyzed their
results according to % of tumor cells stained and levels of staining intensity for all
the protein studied. It would have made for a more in-depth and complete work.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests'
Reviewer's report
Title:Constitutive activation of glycogen synthase kinase-3beta correlates with
better prognosis and cyclin-dependent kinase inhibitors in human gastric cancer
Version: 5Date:13 February 2010
Reviewer: Gregory Kouraklis
Reviewer's report:
The full length article by Yu Jin Cho et al describes the expression of pGSK-3b in
276 human gastric carcinoma speciments.
Other studies have studied the role of GSK-3b in gastric cancer cells.
Today, the role of GSK-3Bb in gastric cancer still remains inconclusive.
The authors of the current study found that GSK-3b activation was frequently
observed in early-stage gastric carcinoma.
Major Compulsory Revisions
1. In the section "Patients", it is written that "The files of 276 surgically resected
gatric cancer examined ....".
In the same section later it is written: "The cases enrolled in this study included
64 advanced and 217 early stage gastric carcinomas".
What is the total number, 276 or 281?
2. It is not stated whether surgical treatment was curative in all patients.
3. It should be stated whether patients received postoperative radio- or
chemotherapy. If additional treatment was given, this must be encounter for the
multivariate analysis.
Discretionary Revisions
1. The authors should state how the sections were assessed. What was the
biological justification (i.e. molecular genetic) for a 10% cutoff for scoring tumors
as positive or negative?
Overall, it would have been advantageous for the authors to have analyzed their
results according to % of tumor cells stained and levels of staining intensity for all
the protein studied. It would have made for a more in-depth and complete work.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests'
Please review this paper.
user
Reviewer's report
Title:Constitutive activation of glycogen synthase kinase-3beta correlates with
better prognosis and cyclin-dependent kinase inhibitors in human gastric cancer
Version: 5Date:13 February 2010
Reviewer: Gregory Kouraklis
Reviewer's report:
The full length article by Yu Jin Cho et al describes the expression of pGSK-3b in
276 human gastric carcinoma speciments.
Other studies have studied the role of GSK-3b in gastric cancer cells.
Today, the role of GSK-3Bb in gastric cancer still remains inconclusive.
The authors of the current study found that GSK-3b activation was frequently
observed in early-stage gastric carcinoma.
Major Compulsory Revisions
1. In the section "Patients", it is written that "The files of 276 surgically resected
gatric cancer examined ....".
In the same section later it is written: "The cases enrolled in this study included
64 advanced and 217 early stage gastric carcinomas".
What is the total number, 276 or 281?
2. It is not stated whether surgical treatment was curative in all patients.
3. It should be stated whether patients received postoperative radio- or
chemotherapy. If additional treatment was given, this must be encounter for the
multivariate analysis.
Discretionary Revisions
1. The authors should state how the sections were assessed. What was the
biological justification (i.e. molecular genetic) for a 10% cutoff for scoring tumors
as positive or negative?
Overall, it would have been advantageous for the authors to have analyzed their
results according to % of tumor cells stained and levels of staining intensity for all
the protein studied. It would have made for a more in-depth and complete work.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests'
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