Title: LUM as a novel prognostic marker and its correlation with immune infiltration in gastric
cancer: a study based on immunohistochemical analysis and bioinformatics
Version: 1 Date: 03 Nov 2023
Reviewer's report:
The authors explored the LUM as a novel prognostic marker in gastric cancer using a series of
bioinformatics methods. And then validated LUM protein expression using IHC. The manuscript
gave a detailed in-depth analysis for LUM in gastric cancer. It is interesting. However, still, I
have some questions.
1. The criteria was set as |log2FC| > 1 (FC = fold change) and a P-value < 0.05, in fact, the
adjusted p values should be used in filtering DEGs for microarray or RNA seq data.
2. I suggest the authors to predict the functions of LUM in gastric cancer, using GSEA or GSVA
to samples dividing by the expression level of LUM.
3. The authors demonstrated that there were significant associations between LUM and the
immune cell infiltration especially macrophage. As I know, there were other genes played the
similar functions in GC. The authors should discuss the possible mechanisms for LUM to affect
the GC progress by promoting macrophage or other immune cells infiltration.
Title: LUM as a novel prognostic marker and its correlation with immune infiltration in gastric
cancer: a study based on immunohistochemical analysis and bioinformatics
Version: 1 Date: 03 Nov 2023
Reviewer's report:
The authors explored the LUM as a novel prognostic marker in gastric cancer using a series of
bioinformatics methods. And then validated LUM protein expression using IHC. The manuscript
gave a detailed in-depth analysis for LUM in gastric cancer. It is interesting. However, still, I
have some questions.
1. The criteria was set as |log2FC| > 1 (FC = fold change) and a P-value < 0.05, in fact, the
adjusted p values should be used in filtering DEGs for microarray or RNA seq data.
2. I suggest the authors to predict the functions of LUM in gastric cancer, using GSEA or GSVA
to samples dividing by the expression level of LUM.
3. The authors demonstrated that there were significant associations between LUM and the
immune cell infiltration especially macrophage. As I know, there were other genes played the
similar functions in GC. The authors should discuss the possible mechanisms for LUM to affect
the GC progress by promoting macrophage or other immune cells infiltration.
Please review this paper.
user
Title: LUM as a novel prognostic marker and its correlation with immune infiltration in gastric
cancer: a study based on immunohistochemical analysis and bioinformatics
Version: 1 Date: 03 Nov 2023
Reviewer's report:
The authors explored the LUM as a novel prognostic marker in gastric cancer using a series of
bioinformatics methods. And then validated LUM protein expression using IHC. The manuscript
gave a detailed in-depth analysis for LUM in gastric cancer. It is interesting. However, still, I
have some questions.
1. The criteria was set as |log2FC| > 1 (FC = fold change) and a P-value < 0.05, in fact, the
adjusted p values should be used in filtering DEGs for microarray or RNA seq data.
2. I suggest the authors to predict the functions of LUM in gastric cancer, using GSEA or GSVA
to samples dividing by the expression level of LUM.
3. The authors demonstrated that there were significant associations between LUM and the
immune cell infiltration especially macrophage. As I know, there were other genes played the
similar functions in GC. The authors should discuss the possible mechanisms for LUM to affect
the GC progress by promoting macrophage or other immune cells infiltration.
assistant