Reviewer ’s report  
Title: Non Alcoholic Fatty Liver disease and eNOS dysfunction in humans  
Version: 0 Date: 07 Nov 2016  
Reviewer: Christa Buechler  
Reviewer's report:  
The article "Non Alcoholic Fatty Liver disease and eNOS dysfunction in humans" by Marcello 
Persico et al demonstrates that eNOS is affected in NAFLD patients.  
 
Manuscript can be improved by addressing the following issues:  
 "Insulin" please use lower case letters (Abstract and text).  
"in both in platelets and in liver" in has to be deleted (Abstract).  
Please correct these and further grammatical / typing errors: "due to the to" (Introduction), 
"p<0,05", "TNF -alfa"  
 
Table 1:  is the BMI of NAFLD patients correct? When looking at BMIs of NAFL and NASH 
this is unlikely. Patients with hypertension are 20 in the whole patients group and 21 when 
groups are listed separately.  
Table 2, please give range of NAS.  
Quality of figure 3  (NAFL and NASH) has to be improved.  
Is there a difference in iNOS activity in the platelets? This should be analyzed in the samples that 
are already available.  
Platelets were treated with insulin and dysfunctional insulin effects on Akt phosphorylatio n 
should be analyzed in these samples.  
"in which it was clearly demonstrated that NAFLD patients had significantly  lower levels of 
s1177 -p-eNOS if compared to both NASH and healthy controls." NAFLD has to be replaced by 
NAFL  
Are the methods appropriate and well described?  
If not, please specify what is required in your comments to the authors.  
 
Yes 
 
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
 
Yes 
 
Are the conclusions drawn adequately supported by the data shown?  
If not, pl ease explain in your comments to the authors.  
 
Yes 
 
Are you able to assess any statistics in the manuscript or would you recommend an  
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects require furth er 
assessment in your comments to the editors.  
 
I am able to assess the statistics  
 
Quality of written English  
Please indicate the quality of language in the manuscript:  
 
Needs some language corrections before being published  
 
Declarati on of competing interests  
Please complete a declaration of competing interests, considering the following questions:  
 
1. Have you in the past five years received reimbursements, fees, funding, or salary from an  
organisation that may in any way gain or lose financially from the publication of this  
manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares in an organisation that may in any way gain or lose  
financially from the publication of this manuscript, either now or in the future?  
 
3. Do you hold or are you currently applying for any patents relating to the content of the  
manuscript?  
 
4. Have you received reimbursements, fees, funding, or salary from an organization that 
holds  or has applied for patents relating to the content of the manu script?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have any non -financial competing interests in relation to this paper?  
 
If you can answer no to all of the above, write 'I declare that I have no competing interests' 
below.  If your re ply is yes to any, please give details below.  
 
I declare that I have no competing interests  
 
I agree to the open peer review policy of the journal. I understand that my name will be included  
on my report to the authors and, if the manuscript is accepted for publication, my named report  
including any attachments I upload will be posted on the website along with the authors'  
responses. I agree for my report to be made available under an Open Access Creative Commons  
CC-BY license ( http://creativecommons.org/lice nses/by/4.0/ ). I understand that any comments  
which I do not wish to be included in my named report can be included as confidential comments  
to the editors, which will not be published.  
 
I agree to the open peer review policy of the journal  
Reviewer ’s report  
Title: Non Alcoholic Fatty Liver disease and eNOS dysfunction in humans  
Version: 0 Date: 07 Nov 2016  
Reviewer: Christa Buechler  
Reviewer's report:  
The article "Non Alcoholic Fatty Liver disease and eNOS dysfunction in humans" by Marcello 
Persico et al demonstrates that eNOS is affected in NAFLD patients.  
 
Manuscript can be improved by addressing the following issues:  
 "Insulin" please use lower case letters (Abstract and text).  
"in both in platelets and in liver" in has to be deleted (Abstract).  
Please correct these and further grammatical / typing errors: "due to the to" (Introduction), 
"p<0,05", "TNF -alfa"  
 
Table 1:  is the BMI of NAFLD patients correct? When looking at BMIs of NAFL and NASH 
this is unlikely. Patients with hypertension are 20 in the whole patients group and 21 when 
groups are listed separately.  
Table 2, please give range of NAS.  
Quality of figure 3  (NAFL and NASH) has to be improved.  
Is there a difference in iNOS activity in the platelets? This should be analyzed in the samples that 
are already available.  
Platelets were treated with insulin and dysfunctional insulin effects on Akt phosphorylatio n 
should be analyzed in these samples.  
"in which it was clearly demonstrated that NAFLD patients had significantly  lower levels of 
s1177 -p-eNOS if compared to both NASH and healthy controls." NAFLD has to be replaced by 
NAFL  
Are the methods appropriate and well described?  
If not, please specify what is required in your comments to the authors.  
 
Yes 
 
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
 
Yes 
 
Are the conclusions drawn adequately supported by the data shown?  
If not, pl ease explain in your comments to the authors.  
 
Yes 
 
Are you able to assess any statistics in the manuscript or would you recommend an  
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects require furth er 
assessment in your comments to the editors.  
 
I am able to assess the statistics  
 
Quality of written English  
Please indicate the quality of language in the manuscript:  
 
Needs some language corrections before being published  
 
Declarati on of competing interests  
Please complete a declaration of competing interests, considering the following questions:  
 
1. Have you in the past five years received reimbursements, fees, funding, or salary from an  
organisation that may in any way gain or lose financially from the publication of this  
manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares in an organisation that may in any way gain or lose  
financially from the publication of this manuscript, either now or in the future?  
 
3. Do you hold or are you currently applying for any patents relating to the content of the  
manuscript?  
 
4. Have you received reimbursements, fees, funding, or salary from an organization that 
holds  or has applied for patents relating to the content of the manu script?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have any non -financial competing interests in relation to this paper?  
 
If you can answer no to all of the above, write 'I declare that I have no competing interests' 
below.  If your re ply is yes to any, please give details below.  
 
I declare that I have no competing interests  
 
I agree to the open peer review policy of the journal. I understand that my name will be included  
on my report to the authors and, if the manuscript is accepted for publication, my named report  
including any attachments I upload will be posted on the website along with the authors'  
responses. I agree for my report to be made available under an Open Access Creative Commons  
CC-BY license ( http://creativecommons.org/lice nses/by/4.0/ ). I understand that any comments  
which I do not wish to be included in my named report can be included as confidential comments  
to the editors, which will not be published.  
 
I agree to the open peer review policy of the journal  
Please review this paper.
user
Reviewer ’s report  
Title: Non Alcoholic Fatty Liver disease and eNOS dysfunction in humans  
Version: 0 Date: 07 Nov 2016  
Reviewer: Christa Buechler  
Reviewer's report:  
The article "Non Alcoholic Fatty Liver disease and eNOS dysfunction in humans" by Marcello 
Persico et al demonstrates that eNOS is affected in NAFLD patients.  
 
Manuscript can be improved by addressing the following issues:  
 "Insulin" please use lower case letters (Abstract and text).  
"in both in platelets and in liver" in has to be deleted (Abstract).  
Please correct these and further grammatical / typing errors: "due to the to" (Introduction), 
"p<0,05", "TNF -alfa"  
 
Table 1:  is the BMI of NAFLD patients correct? When looking at BMIs of NAFL and NASH 
this is unlikely. Patients with hypertension are 20 in the whole patients group and 21 when 
groups are listed separately.  
Table 2, please give range of NAS.  
Quality of figure 3  (NAFL and NASH) has to be improved.  
Is there a difference in iNOS activity in the platelets? This should be analyzed in the samples that 
are already available.  
Platelets were treated with insulin and dysfunctional insulin effects on Akt phosphorylatio n 
should be analyzed in these samples.  
"in which it was clearly demonstrated that NAFLD patients had significantly  lower levels of 
s1177 -p-eNOS if compared to both NASH and healthy controls." NAFLD has to be replaced by 
NAFL  
Are the methods appropriate and well described?  
If not, please specify what is required in your comments to the authors.  
 
Yes 
 
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
 
Yes 
 
Are the conclusions drawn adequately supported by the data shown?  
If not, pl ease explain in your comments to the authors.  
 
Yes 
 
Are you able to assess any statistics in the manuscript or would you recommend an  
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects require furth er 
assessment in your comments to the editors.  
 
I am able to assess the statistics  
 
Quality of written English  
Please indicate the quality of language in the manuscript:  
 
Needs some language corrections before being published  
 
Declarati on of competing interests  
Please complete a declaration of competing interests, considering the following questions:  
 
1. Have you in the past five years received reimbursements, fees, funding, or salary from an  
organisation that may in any way gain or lose financially from the publication of this  
manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares in an organisation that may in any way gain or lose  
financially from the publication of this manuscript, either now or in the future?  
 
3. Do you hold or are you currently applying for any patents relating to the content of the  
manuscript?  
 
4. Have you received reimbursements, fees, funding, or salary from an organization that 
holds  or has applied for patents relating to the content of the manu script?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have any non -financial competing interests in relation to this paper?  
 
If you can answer no to all of the above, write 'I declare that I have no competing interests' 
below.  If your re ply is yes to any, please give details below.  
 
I declare that I have no competing interests  
 
I agree to the open peer review policy of the journal. I understand that my name will be included  
on my report to the authors and, if the manuscript is accepted for publication, my named report  
including any attachments I upload will be posted on the website along with the authors'  
responses. I agree for my report to be made available under an Open Access Creative Commons  
CC-BY license ( http://creativecommons.org/lice nses/by/4.0/ ). I understand that any comments  
which I do not wish to be included in my named report can be included as confidential comments  
to the editors, which will not be published.  
 
I agree to the open peer review policy of the journal  
assistant