Reviewer's report
Title:Expression, localization and polymorphisms of the nuclear receptor PXR in
Barrett's esophagus and esophageal adenocarcinoma
Version: 10Date:13 April 2011
Reviewer: Katerina Dvorak
Reviewer's report:
The authors nicely answered majority of comments, however there few points
that needs to be clarified.
Point 2. LCA and TLCA bind with strong affinity to PXR, however these two bile
acids are present in negligible concentrations in the refluxate. It is not valid
justification for use of these bile acids in the study, even if the PXR expression is
increased in BE and esophageal adenocarcinoma. It should be better explained.
As a justification for not using other bile acids the authors state that other bile
acids as well as a mix of bile acids did not affect PXR mRNA levels. This
argument is not valid, since the data show no effect of TLCA on mRNA levels in
OE19 (Figure3).
Point 4. I do not agree with the statement that western blot analysis would
provide data only on the overall PXR expression. It is possible to separate
subcellular fractions and determine the amount of PXR in cytoplasm or nucleus.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:Expression, localization and polymorphisms of the nuclear receptor PXR in
Barrett's esophagus and esophageal adenocarcinoma
Version: 10Date:13 April 2011
Reviewer: Katerina Dvorak
Reviewer's report:
The authors nicely answered majority of comments, however there few points
that needs to be clarified.
Point 2. LCA and TLCA bind with strong affinity to PXR, however these two bile
acids are present in negligible concentrations in the refluxate. It is not valid
justification for use of these bile acids in the study, even if the PXR expression is
increased in BE and esophageal adenocarcinoma. It should be better explained.
As a justification for not using other bile acids the authors state that other bile
acids as well as a mix of bile acids did not affect PXR mRNA levels. This
argument is not valid, since the data show no effect of TLCA on mRNA levels in
OE19 (Figure3).
Point 4. I do not agree with the statement that western blot analysis would
provide data only on the overall PXR expression. It is possible to separate
subcellular fractions and determine the amount of PXR in cytoplasm or nucleus.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
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Reviewer's report
Title:Expression, localization and polymorphisms of the nuclear receptor PXR in
Barrett's esophagus and esophageal adenocarcinoma
Version: 10Date:13 April 2011
Reviewer: Katerina Dvorak
Reviewer's report:
The authors nicely answered majority of comments, however there few points
that needs to be clarified.
Point 2. LCA and TLCA bind with strong affinity to PXR, however these two bile
acids are present in negligible concentrations in the refluxate. It is not valid
justification for use of these bile acids in the study, even if the PXR expression is
increased in BE and esophageal adenocarcinoma. It should be better explained.
As a justification for not using other bile acids the authors state that other bile
acids as well as a mix of bile acids did not affect PXR mRNA levels. This
argument is not valid, since the data show no effect of TLCA on mRNA levels in
OE19 (Figure3).
Point 4. I do not agree with the statement that western blot analysis would
provide data only on the overall PXR expression. It is possible to separate
subcellular fractions and determine the amount of PXR in cytoplasm or nucleus.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
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