Reviewer's report
Title:Effect of intracellular lipid accumulation in a new model of non-alcoholic
fatty liver disease
Version: 1Date:2 November 2011
Reviewer: Ali Canbay
Reviewer's report:
The manuscript of Chavez-Tapia et al. deals with the effects of intracellular lipid
accumulation in a cell culture model of non-alcoholic fatty liver disease (NAFLD).
HuH7 cells were incubated with different concentrations of free fatty acids
(palmitic and oleic acid at a molar ratio of 1:2) for 24h. After the incubation period
fatty acid uptake, cytotoxic effects, apoptosis, oxidative stress and the release of
inflammatory and fibrogenic cytokines as interleukin-6 and -8, TNF#, TGF-#,
A2M, VEGFA, CTGF, IGF2, NGF and THBS1 were measured. The experiments
could show that free fatty acids play a primary role within the pathogenesis of
NAFLD and NASH by affecting the expression and release of pro-inflammatory
and profibrotic cytokines, enhancing oxidative stress and apoptosis.
The results are reported in a clear and well-organized manner with established
and adequate methods and exactness.
Major Compusory Revisions:
1. As the authors already mentioned using HuH7 cell line demonstrates a limiting
factor. Further experiments with additional hepatic cell lines or ideally with
primary hepatocytes could emphasize and upgrade this study.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:Effect of intracellular lipid accumulation in a new model of non-alcoholic
fatty liver disease
Version: 1Date:2 November 2011
Reviewer: Ali Canbay
Reviewer's report:
The manuscript of Chavez-Tapia et al. deals with the effects of intracellular lipid
accumulation in a cell culture model of non-alcoholic fatty liver disease (NAFLD).
HuH7 cells were incubated with different concentrations of free fatty acids
(palmitic and oleic acid at a molar ratio of 1:2) for 24h. After the incubation period
fatty acid uptake, cytotoxic effects, apoptosis, oxidative stress and the release of
inflammatory and fibrogenic cytokines as interleukin-6 and -8, TNF#, TGF-#,
A2M, VEGFA, CTGF, IGF2, NGF and THBS1 were measured. The experiments
could show that free fatty acids play a primary role within the pathogenesis of
NAFLD and NASH by affecting the expression and release of pro-inflammatory
and profibrotic cytokines, enhancing oxidative stress and apoptosis.
The results are reported in a clear and well-organized manner with established
and adequate methods and exactness.
Major Compusory Revisions:
1. As the authors already mentioned using HuH7 cell line demonstrates a limiting
factor. Further experiments with additional hepatic cell lines or ideally with
primary hepatocytes could emphasize and upgrade this study.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:Effect of intracellular lipid accumulation in a new model of non-alcoholic
fatty liver disease
Version: 1Date:2 November 2011
Reviewer: Ali Canbay
Reviewer's report:
The manuscript of Chavez-Tapia et al. deals with the effects of intracellular lipid
accumulation in a cell culture model of non-alcoholic fatty liver disease (NAFLD).
HuH7 cells were incubated with different concentrations of free fatty acids
(palmitic and oleic acid at a molar ratio of 1:2) for 24h. After the incubation period
fatty acid uptake, cytotoxic effects, apoptosis, oxidative stress and the release of
inflammatory and fibrogenic cytokines as interleukin-6 and -8, TNF#, TGF-#,
A2M, VEGFA, CTGF, IGF2, NGF and THBS1 were measured. The experiments
could show that free fatty acids play a primary role within the pathogenesis of
NAFLD and NASH by affecting the expression and release of pro-inflammatory
and profibrotic cytokines, enhancing oxidative stress and apoptosis.
The results are reported in a clear and well-organized manner with established
and adequate methods and exactness.
Major Compusory Revisions:
1. As the authors already mentioned using HuH7 cell line demonstrates a limiting
factor. Further experiments with additional hepatic cell lines or ideally with
primary hepatocytes could emphasize and upgrade this study.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
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