Title: Analysis of the status and risk factors for Helicobacter pylori infection and drug resistance
in the Lianyungang area, China
Version: 0 Date: 06 Jan 2025
Reviewer:  Fernando P. Monroy
Reviewer's report:
This study used stool samples from residents in Lianyungang to identify the prevalence of H.
pylori infection and resistance to commonly used antibacterial agents and identified potential
risk factors. The manuscript is easy to read and contains all the necessary information, but there
are several concerns that need to be addressed:
1) It would have been easier for the reviewer to have the used line numbers in the text.
2) Throughout the manuscript, please use italics for H. pylori and other genes described.
3) In the abstract, what do you mean by cytotoxin-associated gene A (CagA) and vacuolating
cytotoxin A (VacA) were mutated? Did they express different genotypes (vacA s1,i1,m1; cagA
ABC), or did you base this statement on a comparison to a reference strain?
4) Please provide the reference(s) for the fluorescence PCR melting curve method or list the
primer sequences used in this study.
5) Likewise, provide citations for the nucleic acid mass spectrometry approach.
6) Table 1, under mutation site. Are those commas used? Use italics for genes.
7) Table 2 should be a figure. Also, describe the meaning of attenuated strains. If these strains
lacked both cagA and vacA or only one of these.
8) Similarly, Table 3 should be a figure.
9) In the discussion section, use 13C/14C for isotopes.
10) In the discussion, “Many studies(10-13) have shown ………… Move the (10-13) to the end
of the paragraph.
11) A good read-through for grammatical and style errors throughout the manuscript.
Title: Analysis of the status and risk factors for Helicobacter pylori infection and drug resistance
in the Lianyungang area, China
Version: 0 Date: 06 Jan 2025
Reviewer:  Fernando P. Monroy
Reviewer's report:
This study used stool samples from residents in Lianyungang to identify the prevalence of H.
pylori infection and resistance to commonly used antibacterial agents and identified potential
risk factors. The manuscript is easy to read and contains all the necessary information, but there
are several concerns that need to be addressed:
1) It would have been easier for the reviewer to have the used line numbers in the text.
2) Throughout the manuscript, please use italics for H. pylori and other genes described.
3) In the abstract, what do you mean by cytotoxin-associated gene A (CagA) and vacuolating
cytotoxin A (VacA) were mutated? Did they express different genotypes (vacA s1,i1,m1; cagA
ABC), or did you base this statement on a comparison to a reference strain?
4) Please provide the reference(s) for the fluorescence PCR melting curve method or list the
primer sequences used in this study.
5) Likewise, provide citations for the nucleic acid mass spectrometry approach.
6) Table 1, under mutation site. Are those commas used? Use italics for genes.
7) Table 2 should be a figure. Also, describe the meaning of attenuated strains. If these strains
lacked both cagA and vacA or only one of these.
8) Similarly, Table 3 should be a figure.
9) In the discussion section, use 13C/14C for isotopes.
10) In the discussion, “Many studies(10-13) have shown ………… Move the (10-13) to the end
of the paragraph.
11) A good read-through for grammatical and style errors throughout the manuscript.
Please review this paper.
user
Title: Analysis of the status and risk factors for Helicobacter pylori infection and drug resistance
in the Lianyungang area, China
Version: 0 Date: 06 Jan 2025
Reviewer:  Fernando P. Monroy
Reviewer's report:
This study used stool samples from residents in Lianyungang to identify the prevalence of H.
pylori infection and resistance to commonly used antibacterial agents and identified potential
risk factors. The manuscript is easy to read and contains all the necessary information, but there
are several concerns that need to be addressed:
1) It would have been easier for the reviewer to have the used line numbers in the text.
2) Throughout the manuscript, please use italics for H. pylori and other genes described.
3) In the abstract, what do you mean by cytotoxin-associated gene A (CagA) and vacuolating
cytotoxin A (VacA) were mutated? Did they express different genotypes (vacA s1,i1,m1; cagA
ABC), or did you base this statement on a comparison to a reference strain?
4) Please provide the reference(s) for the fluorescence PCR melting curve method or list the
primer sequences used in this study.
5) Likewise, provide citations for the nucleic acid mass spectrometry approach.
6) Table 1, under mutation site. Are those commas used? Use italics for genes.
7) Table 2 should be a figure. Also, describe the meaning of attenuated strains. If these strains
lacked both cagA and vacA or only one of these.
8) Similarly, Table 3 should be a figure.
9) In the discussion section, use 13C/14C for isotopes.
10) In the discussion, “Many studies(10-13) have shown ………… Move the (10-13) to the end
of the paragraph.
11) A good read-through for grammatical and style errors throughout the manuscript.
assistant