Title: Comparative Serum Imidazole Propionate Profiling in Colorectal Adenoma and Cancer
by UPLC-MS/MS
Version: 0 Date: 06 Apr 2025
Reviewer's report:
The authors evaluate the Imidazole propionate concentration using Ultra-Performance Liquid
Chromatography-Tandem Mass Spectrometry between patients with colorectal adenoma and
colorectal cancer. The objective of your study is acknowledged, and the technique was well
conducted; however, there are methodological concerns.
My comments are as follows.
Major:
1. Your study includes different types of polyps. However, the location plays an important role
since the microbiota is different from right colon to left colon, as same as the histology and the
predisposing risk of CRC since there are different mechanisms related to the development of this
pathology (chromosomal instability (CIN) pathway, microsatellite instability (MSI) pathway,
and CpG island methylator phenotype (CIMP pathway).
To avoid confounding between these distinct endpoints, it would be more appropriate to perform
separate analyses.
If a combined analysis is deemed necessary, clear and strong justification should be provided
for this approach.
Minor:
English Language and Formatting:
The manuscript would benefit from professional English editing to ensure appropriate sentence
structure and readability in medical writing.
It is recommended that the authors obtain professional English editing
Title: Comparative Serum Imidazole Propionate Profiling in Colorectal Adenoma and Cancer
by UPLC-MS/MS
Version: 0 Date: 06 Apr 2025
Reviewer's report:
The authors evaluate the Imidazole propionate concentration using Ultra-Performance Liquid
Chromatography-Tandem Mass Spectrometry between patients with colorectal adenoma and
colorectal cancer. The objective of your study is acknowledged, and the technique was well
conducted; however, there are methodological concerns.
My comments are as follows.
Major:
1. Your study includes different types of polyps. However, the location plays an important role
since the microbiota is different from right colon to left colon, as same as the histology and the
predisposing risk of CRC since there are different mechanisms related to the development of this
pathology (chromosomal instability (CIN) pathway, microsatellite instability (MSI) pathway,
and CpG island methylator phenotype (CIMP pathway).
To avoid confounding between these distinct endpoints, it would be more appropriate to perform
separate analyses.
If a combined analysis is deemed necessary, clear and strong justification should be provided
for this approach.
Minor:
English Language and Formatting:
The manuscript would benefit from professional English editing to ensure appropriate sentence
structure and readability in medical writing.
It is recommended that the authors obtain professional English editing
Please review this paper.
user
Title: Comparative Serum Imidazole Propionate Profiling in Colorectal Adenoma and Cancer
by UPLC-MS/MS
Version: 0 Date: 06 Apr 2025
Reviewer's report:
The authors evaluate the Imidazole propionate concentration using Ultra-Performance Liquid
Chromatography-Tandem Mass Spectrometry between patients with colorectal adenoma and
colorectal cancer. The objective of your study is acknowledged, and the technique was well
conducted; however, there are methodological concerns.
My comments are as follows.
Major:
1. Your study includes different types of polyps. However, the location plays an important role
since the microbiota is different from right colon to left colon, as same as the histology and the
predisposing risk of CRC since there are different mechanisms related to the development of this
pathology (chromosomal instability (CIN) pathway, microsatellite instability (MSI) pathway,
and CpG island methylator phenotype (CIMP pathway).
To avoid confounding between these distinct endpoints, it would be more appropriate to perform
separate analyses.
If a combined analysis is deemed necessary, clear and strong justification should be provided
for this approach.
Minor:
English Language and Formatting:
The manuscript would benefit from professional English editing to ensure appropriate sentence
structure and readability in medical writing.
It is recommended that the authors obtain professional English editing
assistant