Title: Causal Relationship Between Major Depressive Disorder, Anxiety Disorder and
Constipation: A Two-sample Mendelian randomization study
Version: 1 Date: 30 Oct 2024
Reviewer's report:
PEER REVIEWER ASSESSMENTS:
OBJECTIVE - Full research articles: is there a clear objective that addresses one or several
testable research questions? (Brief or other article types: is there a clear objective?)
Yes - there is a clear objective
DESIGN - Is the current approach (including controls and analysis protocols) appropriate for
the objective?
Yes - the approach is appropriate
EXECUTION - Are the experiments and analyses performed with sufficient technical rigor to
allow confidence in the results?
Yes - experiments and analyses were performed appropriately
STATISTICS - Is the use of statistics in the manuscript appropriate?
Yes - appropriate statistical analyses have been used in the study
INTERPRETATION - Is the current interpretation/discussion of the results reasonable and not
overstated?
Yes - the author's interpretation is reasonable
OVERALL MANUSCRIPT POTENTIAL - Has the author addressed your concerns sufficiently
for you to now recommend the work as a technically sound contribution? If not, can further
revisions be made to make the work technically sound?
Yes - current version is technically sound
PEER REVIEWER COMMENTS:
GENERAL COMMENTS: This is a repeat review of the manuscript titled "Causal
Relationship Between Major Depressive Disorder, Anxiety Disorder and Constipation: A Two-
sample Mendelian randomization study". Authors used Mendelian randomization (MR) to
extract significant causal relationships between anxiety phenotypes, depression phenotypes,
constipation, and have also discussed contribution of varius microbial species of the gut to each
of these phenotypes. MS has greatly improved since last review, and is ready to be published now
ADDITIONAL REQUESTS/SUGGESTIONS:
Thank you for doing the corrections highlighted by our previous review and be Rev 1
Title: Causal Relationship Between Major Depressive Disorder, Anxiety Disorder and
Constipation: A Two-sample Mendelian randomization study
Version: 1 Date: 30 Oct 2024
Reviewer's report:
PEER REVIEWER ASSESSMENTS:
OBJECTIVE - Full research articles: is there a clear objective that addresses one or several
testable research questions? (Brief or other article types: is there a clear objective?)
Yes - there is a clear objective
DESIGN - Is the current approach (including controls and analysis protocols) appropriate for
the objective?
Yes - the approach is appropriate
EXECUTION - Are the experiments and analyses performed with sufficient technical rigor to
allow confidence in the results?
Yes - experiments and analyses were performed appropriately
STATISTICS - Is the use of statistics in the manuscript appropriate?
Yes - appropriate statistical analyses have been used in the study
INTERPRETATION - Is the current interpretation/discussion of the results reasonable and not
overstated?
Yes - the author's interpretation is reasonable
OVERALL MANUSCRIPT POTENTIAL - Has the author addressed your concerns sufficiently
for you to now recommend the work as a technically sound contribution? If not, can further
revisions be made to make the work technically sound?
Yes - current version is technically sound
PEER REVIEWER COMMENTS:
GENERAL COMMENTS: This is a repeat review of the manuscript titled "Causal
Relationship Between Major Depressive Disorder, Anxiety Disorder and Constipation: A Two-
sample Mendelian randomization study". Authors used Mendelian randomization (MR) to
extract significant causal relationships between anxiety phenotypes, depression phenotypes,
constipation, and have also discussed contribution of varius microbial species of the gut to each
of these phenotypes. MS has greatly improved since last review, and is ready to be published now
ADDITIONAL REQUESTS/SUGGESTIONS:
Thank you for doing the corrections highlighted by our previous review and be Rev 1
Please review this paper.
user
Title: Causal Relationship Between Major Depressive Disorder, Anxiety Disorder and
Constipation: A Two-sample Mendelian randomization study
Version: 1 Date: 30 Oct 2024
Reviewer's report:
PEER REVIEWER ASSESSMENTS:
OBJECTIVE - Full research articles: is there a clear objective that addresses one or several
testable research questions? (Brief or other article types: is there a clear objective?)
Yes - there is a clear objective
DESIGN - Is the current approach (including controls and analysis protocols) appropriate for
the objective?
Yes - the approach is appropriate
EXECUTION - Are the experiments and analyses performed with sufficient technical rigor to
allow confidence in the results?
Yes - experiments and analyses were performed appropriately
STATISTICS - Is the use of statistics in the manuscript appropriate?
Yes - appropriate statistical analyses have been used in the study
INTERPRETATION - Is the current interpretation/discussion of the results reasonable and not
overstated?
Yes - the author's interpretation is reasonable
OVERALL MANUSCRIPT POTENTIAL - Has the author addressed your concerns sufficiently
for you to now recommend the work as a technically sound contribution? If not, can further
revisions be made to make the work technically sound?
Yes - current version is technically sound
PEER REVIEWER COMMENTS:
GENERAL COMMENTS: This is a repeat review of the manuscript titled "Causal
Relationship Between Major Depressive Disorder, Anxiety Disorder and Constipation: A Two-
sample Mendelian randomization study". Authors used Mendelian randomization (MR) to
extract significant causal relationships between anxiety phenotypes, depression phenotypes,
constipation, and have also discussed contribution of varius microbial species of the gut to each
of these phenotypes. MS has greatly improved since last review, and is ready to be published now
ADDITIONAL REQUESTS/SUGGESTIONS:
Thank you for doing the corrections highlighted by our previous review and be Rev 1
assistant