Title: Discriminant equation using mucosally expressed cytokines and transcription factor for
making definite diagnosis of inflammatory bowel disease unclassified
Version: 1 Date: 15 Dec 2020
Reviewer's report:
Okuno et al. reported the results of the Discriminant analysis using mucosally expressed
cytokines and transcription factors to conduct a definitive diagnosis of inflammatory bowel
disease unclassified. The study is crucial as it demonstrates the discriminant analysis of
differential diagnosis of Ulcerative Colitis (UC) or Crohn’s Disease (CD) for Inflammatory
bowel disease unclassified (IBD-U) using mucosally expressed cytokines.
The results demonstrate that the discriminant equation using mucosally expressed cytokines can
be a diagnostic tool for a subtype of IBD; however, there are several concerns that need to be
addressed.
1. The authors should also note the clinical course of IBD-U patients, such as the duration it took
to diagnose each case with UC or CD. When were upper gastrointestinal endoscopy and small
intestinal examination including barium enema conducted? Were the diagnostic tests conducted
during the first visit for all cases?
2. The authors explained the reasons for why the three-parameter discriminant equation
displayed a high probability of 90%, but since 70% (7/10) of the final diagnosis in IBD-U was
CD, can this bias affect the result? In Fig 3B, in all situations of CD, only three-parameter
discriminant equations are zero or less. Please clarify.
3. The authors mentioned that the expression of inflammatory genes in patients with IBD-U can
overlap more frequently than conventional IBDs. Please mention the rationale for differential
expression patterns of inflammatory genes between IBD-U and conventional IBDs.
4. On page 9: Table S2 appears to be the same as Table S1. Please clarify.
5. In Table 1, Disease types contains two examples of Pancolitis. Please comfirm.
6. Please check the description method of reference 8 on page 17.
(Th1 response8 #Th1 response [8])
Title: Discriminant equation using mucosally expressed cytokines and transcription factor for
making definite diagnosis of inflammatory bowel disease unclassified
Version: 1 Date: 15 Dec 2020
Reviewer's report:
Okuno et al. reported the results of the Discriminant analysis using mucosally expressed
cytokines and transcription factors to conduct a definitive diagnosis of inflammatory bowel
disease unclassified. The study is crucial as it demonstrates the discriminant analysis of
differential diagnosis of Ulcerative Colitis (UC) or Crohn’s Disease (CD) for Inflammatory
bowel disease unclassified (IBD-U) using mucosally expressed cytokines.
The results demonstrate that the discriminant equation using mucosally expressed cytokines can
be a diagnostic tool for a subtype of IBD; however, there are several concerns that need to be
addressed.
1. The authors should also note the clinical course of IBD-U patients, such as the duration it took
to diagnose each case with UC or CD. When were upper gastrointestinal endoscopy and small
intestinal examination including barium enema conducted? Were the diagnostic tests conducted
during the first visit for all cases?
2. The authors explained the reasons for why the three-parameter discriminant equation
displayed a high probability of 90%, but since 70% (7/10) of the final diagnosis in IBD-U was
CD, can this bias affect the result? In Fig 3B, in all situations of CD, only three-parameter
discriminant equations are zero or less. Please clarify.
3. The authors mentioned that the expression of inflammatory genes in patients with IBD-U can
overlap more frequently than conventional IBDs. Please mention the rationale for differential
expression patterns of inflammatory genes between IBD-U and conventional IBDs.
4. On page 9: Table S2 appears to be the same as Table S1. Please clarify.
5. In Table 1, Disease types contains two examples of Pancolitis. Please comfirm.
6. Please check the description method of reference 8 on page 17.
(Th1 response8 #Th1 response [8])
Please review this paper.
user
Title: Discriminant equation using mucosally expressed cytokines and transcription factor for
making definite diagnosis of inflammatory bowel disease unclassified
Version: 1 Date: 15 Dec 2020
Reviewer's report:
Okuno et al. reported the results of the Discriminant analysis using mucosally expressed
cytokines and transcription factors to conduct a definitive diagnosis of inflammatory bowel
disease unclassified. The study is crucial as it demonstrates the discriminant analysis of
differential diagnosis of Ulcerative Colitis (UC) or Crohn’s Disease (CD) for Inflammatory
bowel disease unclassified (IBD-U) using mucosally expressed cytokines.
The results demonstrate that the discriminant equation using mucosally expressed cytokines can
be a diagnostic tool for a subtype of IBD; however, there are several concerns that need to be
addressed.
1. The authors should also note the clinical course of IBD-U patients, such as the duration it took
to diagnose each case with UC or CD. When were upper gastrointestinal endoscopy and small
intestinal examination including barium enema conducted? Were the diagnostic tests conducted
during the first visit for all cases?
2. The authors explained the reasons for why the three-parameter discriminant equation
displayed a high probability of 90%, but since 70% (7/10) of the final diagnosis in IBD-U was
CD, can this bias affect the result? In Fig 3B, in all situations of CD, only three-parameter
discriminant equations are zero or less. Please clarify.
3. The authors mentioned that the expression of inflammatory genes in patients with IBD-U can
overlap more frequently than conventional IBDs. Please mention the rationale for differential
expression patterns of inflammatory genes between IBD-U and conventional IBDs.
4. On page 9: Table S2 appears to be the same as Table S1. Please clarify.
5. In Table 1, Disease types contains two examples of Pancolitis. Please comfirm.
6. Please check the description method of reference 8 on page 17.
(Th1 response8 #Th1 response [8])
assistant