Reviewer's report
Title:Heat Shock Protein-27, -60 and -90 expression in gastric cancer:
association with clinicopathological variables and patient survival
Version: 1Date:8 May 2008
Reviewer: Gabriele Multhoff
Reviewer's report:
BMC Gastroentereology
MS by Stamatios Theocharis
The findings in the Ms reveal an association of Hsp27, 60 and 90 expression in
gastric cancer (n=66) with clinicoapthological parameters. The data are
predominantly based on immunohistochemical studies.
In the discussion part the authors also include data from Hsp70 analyses,
however these data are not shown as results in the manuscript.
The data are important with respect to the identification of new prognostic
markers. However, the Ms would improve by including some additional
informations
Figure 1: Could data of stainings with isotype-matched control antibodies be
included
Is it possible to show data for Hsp70 and Hsp40 immunohistochemical analyses.
What is the association between intracellular and extracellular HSPs.
Is it possible to show examples of high and low expression of the HSPs in
comparison.
Did the authors measure the HSPs in the serum of the patients and if so is there
a link between intracellular and extarcellular HSP levels.
Discussion: Could the authors provide some more ideas about the physiological
role of the increased HSP levels in gastric cancer.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competeing interests
Reviewer's report
Title:Heat Shock Protein-27, -60 and -90 expression in gastric cancer:
association with clinicopathological variables and patient survival
Version: 1Date:8 May 2008
Reviewer: Gabriele Multhoff
Reviewer's report:
BMC Gastroentereology
MS by Stamatios Theocharis
The findings in the Ms reveal an association of Hsp27, 60 and 90 expression in
gastric cancer (n=66) with clinicoapthological parameters. The data are
predominantly based on immunohistochemical studies.
In the discussion part the authors also include data from Hsp70 analyses,
however these data are not shown as results in the manuscript.
The data are important with respect to the identification of new prognostic
markers. However, the Ms would improve by including some additional
informations
Figure 1: Could data of stainings with isotype-matched control antibodies be
included
Is it possible to show data for Hsp70 and Hsp40 immunohistochemical analyses.
What is the association between intracellular and extracellular HSPs.
Is it possible to show examples of high and low expression of the HSPs in
comparison.
Did the authors measure the HSPs in the serum of the patients and if so is there
a link between intracellular and extarcellular HSP levels.
Discussion: Could the authors provide some more ideas about the physiological
role of the increased HSP levels in gastric cancer.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competeing interests
Please review this paper.
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Reviewer's report
Title:Heat Shock Protein-27, -60 and -90 expression in gastric cancer:
association with clinicopathological variables and patient survival
Version: 1Date:8 May 2008
Reviewer: Gabriele Multhoff
Reviewer's report:
BMC Gastroentereology
MS by Stamatios Theocharis
The findings in the Ms reveal an association of Hsp27, 60 and 90 expression in
gastric cancer (n=66) with clinicoapthological parameters. The data are
predominantly based on immunohistochemical studies.
In the discussion part the authors also include data from Hsp70 analyses,
however these data are not shown as results in the manuscript.
The data are important with respect to the identification of new prognostic
markers. However, the Ms would improve by including some additional
informations
Figure 1: Could data of stainings with isotype-matched control antibodies be
included
Is it possible to show data for Hsp70 and Hsp40 immunohistochemical analyses.
What is the association between intracellular and extracellular HSPs.
Is it possible to show examples of high and low expression of the HSPs in
comparison.
Did the authors measure the HSPs in the serum of the patients and if so is there
a link between intracellular and extarcellular HSP levels.
Discussion: Could the authors provide some more ideas about the physiological
role of the increased HSP levels in gastric cancer.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competeing interests
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