Reviewer's report
Title:Paraoxonase-1 is related to inflammation, fibrosis and PPAR delta in
experimental liver disease
Version: 1Date:24 October 2008
Reviewer: Fabio Marra
Reviewer's report:
MAJOR COMPULSORY REVISIONS
1. No explanation is provided as to why mRNA levels drop and protein is
increased. After 12 weeks, protein levels should follow those of steady-state
mRNA levels, otherwise one of the two determinations may be not reliable. The
intrahepatic levels of PON1 must be reassessed using an alternative method, to
confirm the present results.
2. No direct proof of the interaction between MCP-1 and PON1 is provided. It is
said that the two proteins are directly related, but actually in the discussion it is
stated that when PON1 is high, MCP-1 is low (page 10). How should one
understand? Similarly, the possible regulation of PON1 by PPAR-delta or
cathepsin B is only hypothetical, and this should be clearly stated. It is said that
cathepsin B decreased during CCl4 administration, but I could not gather this
information from Figure 4.
MINOR ESSENTIAL REVISIONS
1. The role of PON1 has been previously shown in the liver, including data with
PON1 overexpression. It should be more clearly stated to what extent this study
provides novel information
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Paraoxonase-1 is related to inflammation, fibrosis and PPAR delta in
experimental liver disease
Version: 1Date:24 October 2008
Reviewer: Fabio Marra
Reviewer's report:
MAJOR COMPULSORY REVISIONS
1. No explanation is provided as to why mRNA levels drop and protein is
increased. After 12 weeks, protein levels should follow those of steady-state
mRNA levels, otherwise one of the two determinations may be not reliable. The
intrahepatic levels of PON1 must be reassessed using an alternative method, to
confirm the present results.
2. No direct proof of the interaction between MCP-1 and PON1 is provided. It is
said that the two proteins are directly related, but actually in the discussion it is
stated that when PON1 is high, MCP-1 is low (page 10). How should one
understand? Similarly, the possible regulation of PON1 by PPAR-delta or
cathepsin B is only hypothetical, and this should be clearly stated. It is said that
cathepsin B decreased during CCl4 administration, but I could not gather this
information from Figure 4.
MINOR ESSENTIAL REVISIONS
1. The role of PON1 has been previously shown in the liver, including data with
PON1 overexpression. It should be more clearly stated to what extent this study
provides novel information
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Paraoxonase-1 is related to inflammation, fibrosis and PPAR delta in
experimental liver disease
Version: 1Date:24 October 2008
Reviewer: Fabio Marra
Reviewer's report:
MAJOR COMPULSORY REVISIONS
1. No explanation is provided as to why mRNA levels drop and protein is
increased. After 12 weeks, protein levels should follow those of steady-state
mRNA levels, otherwise one of the two determinations may be not reliable. The
intrahepatic levels of PON1 must be reassessed using an alternative method, to
confirm the present results.
2. No direct proof of the interaction between MCP-1 and PON1 is provided. It is
said that the two proteins are directly related, but actually in the discussion it is
stated that when PON1 is high, MCP-1 is low (page 10). How should one
understand? Similarly, the possible regulation of PON1 by PPAR-delta or
cathepsin B is only hypothetical, and this should be clearly stated. It is said that
cathepsin B decreased during CCl4 administration, but I could not gather this
information from Figure 4.
MINOR ESSENTIAL REVISIONS
1. The role of PON1 has been previously shown in the liver, including data with
PON1 overexpression. It should be more clearly stated to what extent this study
provides novel information
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
assistant