Title: Integration of bile proteomics and metabolomics analyses reveals novel insights into
different types of gallstones in a high-altitude area
Version: 3 Date: 15 Aug 2024
Reviewer's report:
I have read the manuscript carefully.
The study used proteomics and metabolomics to genomically analyse the bile of patients with
two types of gallstones, which has some clinical significance, however, I have the following
specific comments on this manuscript#
1#In the overall experimental design it was stated that this study included 20 patients with CGD
and 30 patients with PGD, however the bile collection process was described as collecting bile
specimens from 40 patients in both groups after surgery. What about the other 10 patients?
2#Metabolomics took 20 specimens from each group of patients and 9 specimens from each
group for proteomics, however, this was not stated in the paper.
3#The results of the study are not described in detail as to how the metabolome and proteome
were correlated and analysed, the conclusions are not detailed enough, the overall analysis is
too simple, and the images are too simple and not clear enough.
4#Proteomics and metabolomics testing companies should be described in the paper.
Title: Integration of bile proteomics and metabolomics analyses reveals novel insights into
different types of gallstones in a high-altitude area
Version: 3 Date: 15 Aug 2024
Reviewer's report:
I have read the manuscript carefully.
The study used proteomics and metabolomics to genomically analyse the bile of patients with
two types of gallstones, which has some clinical significance, however, I have the following
specific comments on this manuscript#
1#In the overall experimental design it was stated that this study included 20 patients with CGD
and 30 patients with PGD, however the bile collection process was described as collecting bile
specimens from 40 patients in both groups after surgery. What about the other 10 patients?
2#Metabolomics took 20 specimens from each group of patients and 9 specimens from each
group for proteomics, however, this was not stated in the paper.
3#The results of the study are not described in detail as to how the metabolome and proteome
were correlated and analysed, the conclusions are not detailed enough, the overall analysis is
too simple, and the images are too simple and not clear enough.
4#Proteomics and metabolomics testing companies should be described in the paper.
Please review this paper.
user
Title: Integration of bile proteomics and metabolomics analyses reveals novel insights into
different types of gallstones in a high-altitude area
Version: 3 Date: 15 Aug 2024
Reviewer's report:
I have read the manuscript carefully.
The study used proteomics and metabolomics to genomically analyse the bile of patients with
two types of gallstones, which has some clinical significance, however, I have the following
specific comments on this manuscript#
1#In the overall experimental design it was stated that this study included 20 patients with CGD
and 30 patients with PGD, however the bile collection process was described as collecting bile
specimens from 40 patients in both groups after surgery. What about the other 10 patients?
2#Metabolomics took 20 specimens from each group of patients and 9 specimens from each
group for proteomics, however, this was not stated in the paper.
3#The results of the study are not described in detail as to how the metabolome and proteome
were correlated and analysed, the conclusions are not detailed enough, the overall analysis is
too simple, and the images are too simple and not clear enough.
4#Proteomics and metabolomics testing companies should be described in the paper.
assistant