Title: The relationship between innate/adaptive immunity and gastrointestinal cancer : a multi-
omics Mendelian randomization study
Version: 0 Date: 25 May 2024
Reviewer's report:
Review comments
This manuscript presents very interesting data on the association between immune-related genes
and GI cancer. The authors used databases and published data to identify HLA-DRA genes and
methylation sites that regulate HLA-DRA gene expression and clarified their association with
gastric cancer risk. However, several questions arise from this study, and I would like to ask the
authors to provide appropriate answers.
1. Expression of HLA-DRA has also been found in colorectal cancer and has been shown to
correlate with tumor infiltrating lymphocyte (TIL). What was the correlation between HLA-
DRA expression and TILs and other clinicopathological factors in this study?
2. The authors showed by single-cell expression analysis that HLA-DRA expression is enriched
in macrophages and myeloid cells in gastric cancer tissue. What do you think is the effects of
this high expression of HLA-DRA in the gastric cancer microenvironment on carcinogenesis
and prognosis of gastric cancer?
3. The authors identified methylation site (cg17294865) associated with gastric cancer risk.
Where is this methylation site in the HLA-DRA gene? Promoter region?
Title: The relationship between innate/adaptive immunity and gastrointestinal cancer : a multi-
omics Mendelian randomization study
Version: 0 Date: 25 May 2024
Reviewer's report:
Review comments
This manuscript presents very interesting data on the association between immune-related genes
and GI cancer. The authors used databases and published data to identify HLA-DRA genes and
methylation sites that regulate HLA-DRA gene expression and clarified their association with
gastric cancer risk. However, several questions arise from this study, and I would like to ask the
authors to provide appropriate answers.
1. Expression of HLA-DRA has also been found in colorectal cancer and has been shown to
correlate with tumor infiltrating lymphocyte (TIL). What was the correlation between HLA-
DRA expression and TILs and other clinicopathological factors in this study?
2. The authors showed by single-cell expression analysis that HLA-DRA expression is enriched
in macrophages and myeloid cells in gastric cancer tissue. What do you think is the effects of
this high expression of HLA-DRA in the gastric cancer microenvironment on carcinogenesis
and prognosis of gastric cancer?
3. The authors identified methylation site (cg17294865) associated with gastric cancer risk.
Where is this methylation site in the HLA-DRA gene? Promoter region?
Please review this paper.
user
Title: The relationship between innate/adaptive immunity and gastrointestinal cancer : a multi-
omics Mendelian randomization study
Version: 0 Date: 25 May 2024
Reviewer's report:
Review comments
This manuscript presents very interesting data on the association between immune-related genes
and GI cancer. The authors used databases and published data to identify HLA-DRA genes and
methylation sites that regulate HLA-DRA gene expression and clarified their association with
gastric cancer risk. However, several questions arise from this study, and I would like to ask the
authors to provide appropriate answers.
1. Expression of HLA-DRA has also been found in colorectal cancer and has been shown to
correlate with tumor infiltrating lymphocyte (TIL). What was the correlation between HLA-
DRA expression and TILs and other clinicopathological factors in this study?
2. The authors showed by single-cell expression analysis that HLA-DRA expression is enriched
in macrophages and myeloid cells in gastric cancer tissue. What do you think is the effects of
this high expression of HLA-DRA in the gastric cancer microenvironment on carcinogenesis
and prognosis of gastric cancer?
3. The authors identified methylation site (cg17294865) associated with gastric cancer risk.
Where is this methylation site in the HLA-DRA gene? Promoter region?
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