Reviewer's report
Title:The roles of tumor necrosis factor-alpha in colon tight junction protein
expression and intestinal mucosa structure in a mouse model of acute liver
failure
Version: 4Date:2 July 2009
Reviewer: Hiroyasu Ito
Reviewer's report:
Comments;
In this manuscript, the authors have investigated the effects of TNF-# on the
ultrastructure of the intestinal mucosa using acute liver failure murine model.
They demonstrated that TNF-# damages TJs and affects the expression of ZO-1
protein in vivo, and may participate in the pathological mechanism of SBP
complicated to ALF. Although the present data are potentially easily
understandable and very interesting, there are several issues that should be
addressed as noted below.
Major;
1, Authors demonstrated that TNF-# damages TJs and decreases the expression
of ZO-1 protein in vivo. However, in the group in which only mice was
administrated with recombinant TNF-#, there was no damage on TJs and the
expression of ZO-1 was not decrease. If high dose of recombinant TNF-# was
injected to mice, is it observed the damage of TJs and the decrease of the
expression of ZO-1?
Authors should discuss on these issues.
2, In Western blot analysis of tissue ZO-1 content, the author should show the
internal control protein expression (e.g. #-actin, GAPDH etc.) to normalize for
protein loading. (Fig. 5)
3, In Fig.1, the concentration of TNF-# level was elevated at 9 hour after
GalN/LPS injection again. Why are observed these bimodal peaks of the
concentration of TNF-# level after GalN/LPS injection?
Authors should address this issues in Discussion.
Minor;
1, In Table 1, number of mice in the groups of anti-TNF-#-IgG+GalN/LPS and
anti-TNF-#R-IgG+GalN/LPS might be wrong.
2, The description `TNF-a` at line 32 in Discussion might be wrong.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:The roles of tumor necrosis factor-alpha in colon tight junction protein
expression and intestinal mucosa structure in a mouse model of acute liver
failure
Version: 4Date:2 July 2009
Reviewer: Hiroyasu Ito
Reviewer's report:
Comments;
In this manuscript, the authors have investigated the effects of TNF-# on the
ultrastructure of the intestinal mucosa using acute liver failure murine model.
They demonstrated that TNF-# damages TJs and affects the expression of ZO-1
protein in vivo, and may participate in the pathological mechanism of SBP
complicated to ALF. Although the present data are potentially easily
understandable and very interesting, there are several issues that should be
addressed as noted below.
Major;
1, Authors demonstrated that TNF-# damages TJs and decreases the expression
of ZO-1 protein in vivo. However, in the group in which only mice was
administrated with recombinant TNF-#, there was no damage on TJs and the
expression of ZO-1 was not decrease. If high dose of recombinant TNF-# was
injected to mice, is it observed the damage of TJs and the decrease of the
expression of ZO-1?
Authors should discuss on these issues.
2, In Western blot analysis of tissue ZO-1 content, the author should show the
internal control protein expression (e.g. #-actin, GAPDH etc.) to normalize for
protein loading. (Fig. 5)
3, In Fig.1, the concentration of TNF-# level was elevated at 9 hour after
GalN/LPS injection again. Why are observed these bimodal peaks of the
concentration of TNF-# level after GalN/LPS injection?
Authors should address this issues in Discussion.
Minor;
1, In Table 1, number of mice in the groups of anti-TNF-#-IgG+GalN/LPS and
anti-TNF-#R-IgG+GalN/LPS might be wrong.
2, The description `TNF-a` at line 32 in Discussion might be wrong.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:The roles of tumor necrosis factor-alpha in colon tight junction protein
expression and intestinal mucosa structure in a mouse model of acute liver
failure
Version: 4Date:2 July 2009
Reviewer: Hiroyasu Ito
Reviewer's report:
Comments;
In this manuscript, the authors have investigated the effects of TNF-# on the
ultrastructure of the intestinal mucosa using acute liver failure murine model.
They demonstrated that TNF-# damages TJs and affects the expression of ZO-1
protein in vivo, and may participate in the pathological mechanism of SBP
complicated to ALF. Although the present data are potentially easily
understandable and very interesting, there are several issues that should be
addressed as noted below.
Major;
1, Authors demonstrated that TNF-# damages TJs and decreases the expression
of ZO-1 protein in vivo. However, in the group in which only mice was
administrated with recombinant TNF-#, there was no damage on TJs and the
expression of ZO-1 was not decrease. If high dose of recombinant TNF-# was
injected to mice, is it observed the damage of TJs and the decrease of the
expression of ZO-1?
Authors should discuss on these issues.
2, In Western blot analysis of tissue ZO-1 content, the author should show the
internal control protein expression (e.g. #-actin, GAPDH etc.) to normalize for
protein loading. (Fig. 5)
3, In Fig.1, the concentration of TNF-# level was elevated at 9 hour after
GalN/LPS injection again. Why are observed these bimodal peaks of the
concentration of TNF-# level after GalN/LPS injection?
Authors should address this issues in Discussion.
Minor;
1, In Table 1, number of mice in the groups of anti-TNF-#-IgG+GalN/LPS and
anti-TNF-#R-IgG+GalN/LPS might be wrong.
2, The description `TNF-a` at line 32 in Discussion might be wrong.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
assistant