Reviewer's report
Title:Hepatoprotective role of liver fatty acid binding protein in acetaminophen
induced toxicity
Version: 2Date:15 July 2013
Reviewer: akif alt&#305;nbas
Reviewer's report:
The authors investigated the role of FABP1 in acetominophen treated Chang cell
line. Why Chang cell has no FAPP1 expression, it seems to be ideal in assesing
the exact role of FABP1 in AAP induced liver injury in cell cultere model.
Moreover, the crucial pathogenesis of AAP related liver injury is thought to be
related qith oxidant stress, and FABP1 was shown to have anti-axidant
properties.
The authors investigated not only cell viability, but also the degree of cell lysis by
LDH measurement, and apoptosis by BAX measurement, a pr-apoptotic protein,
in different doses of AAP treated cell lines. They have founded that with FABP1
treatment, the cell death process, in the begining apoptosis and the latter phases
necrosis, was improved in different stages, both in early and late stages.
I do not have any major or minor criticism.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Hepatoprotective role of liver fatty acid binding protein in acetaminophen
induced toxicity
Version: 2Date:15 July 2013
Reviewer: akif alt&#305;nbas
Reviewer's report:
The authors investigated the role of FABP1 in acetominophen treated Chang cell
line. Why Chang cell has no FAPP1 expression, it seems to be ideal in assesing
the exact role of FABP1 in AAP induced liver injury in cell cultere model.
Moreover, the crucial pathogenesis of AAP related liver injury is thought to be
related qith oxidant stress, and FABP1 was shown to have anti-axidant
properties.
The authors investigated not only cell viability, but also the degree of cell lysis by
LDH measurement, and apoptosis by BAX measurement, a pr-apoptotic protein,
in different doses of AAP treated cell lines. They have founded that with FABP1
treatment, the cell death process, in the begining apoptosis and the latter phases
necrosis, was improved in different stages, both in early and late stages.
I do not have any major or minor criticism.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Hepatoprotective role of liver fatty acid binding protein in acetaminophen
induced toxicity
Version: 2Date:15 July 2013
Reviewer: akif alt&#305;nbas
Reviewer's report:
The authors investigated the role of FABP1 in acetominophen treated Chang cell
line. Why Chang cell has no FAPP1 expression, it seems to be ideal in assesing
the exact role of FABP1 in AAP induced liver injury in cell cultere model.
Moreover, the crucial pathogenesis of AAP related liver injury is thought to be
related qith oxidant stress, and FABP1 was shown to have anti-axidant
properties.
The authors investigated not only cell viability, but also the degree of cell lysis by
LDH measurement, and apoptosis by BAX measurement, a pr-apoptotic protein,
in different doses of AAP treated cell lines. They have founded that with FABP1
treatment, the cell death process, in the begining apoptosis and the latter phases
necrosis, was improved in different stages, both in early and late stages.
I do not have any major or minor criticism.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
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