Reviewer's report
Title:Luteolin decreases IGF-II production and down-regulates insulin-like
growth factor-I receptor signaling in HT-29 human colon cancer cells
Version: 1Date:29 July 2011
Reviewer: Sang Woon Choi
Reviewer's report:
Major Compulsory Revisions
1) Page 2: The method section of the abstract needs to be changed. Authors just
listed the names of method and did not address what they measured.
2) Page 12, Line 4: ‘IGF-1 stimulated the association of the p58 regulatory….’
What is ‘the association’? Please make it clear.
3) The conclusion section includes only a brief summary of the results. The
statement of significance of the results or the future perspectives could be
needed.
Minor Essential Revisions
1) Page 2: The method section of the abstract: What does MTT stands for? If the
method is just a routine one for cell culture work, it doesn’t need to be here.
Discretionary Revisions
1) Does HT-29 cell also increase the synthesis of IGF-I? If so, can it also be
inhibited by luteolin?
2) Would it be reasonable to test if IGF II stimulated HT-29 cell growth also does
not abrogate the luteolin-induced growth inhibition?
3) Authors used only one cancer cell line. It is curious whether or not luteolin also
inhibits normal cell’s IGF-IR signaling. If so, too much luteolin could be bad for
the normal cells, even though it is good to inhibit the cancer cell growth?
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:Luteolin decreases IGF-II production and down-regulates insulin-like
growth factor-I receptor signaling in HT-29 human colon cancer cells
Version: 1Date:29 July 2011
Reviewer: Sang Woon Choi
Reviewer's report:
Major Compulsory Revisions
1) Page 2: The method section of the abstract needs to be changed. Authors just
listed the names of method and did not address what they measured.
2) Page 12, Line 4: ‘IGF-1 stimulated the association of the p58 regulatory….’
What is ‘the association’? Please make it clear.
3) The conclusion section includes only a brief summary of the results. The
statement of significance of the results or the future perspectives could be
needed.
Minor Essential Revisions
1) Page 2: The method section of the abstract: What does MTT stands for? If the
method is just a routine one for cell culture work, it doesn’t need to be here.
Discretionary Revisions
1) Does HT-29 cell also increase the synthesis of IGF-I? If so, can it also be
inhibited by luteolin?
2) Would it be reasonable to test if IGF II stimulated HT-29 cell growth also does
not abrogate the luteolin-induced growth inhibition?
3) Authors used only one cancer cell line. It is curious whether or not luteolin also
inhibits normal cell’s IGF-IR signaling. If so, too much luteolin could be bad for
the normal cells, even though it is good to inhibit the cancer cell growth?
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:Luteolin decreases IGF-II production and down-regulates insulin-like
growth factor-I receptor signaling in HT-29 human colon cancer cells
Version: 1Date:29 July 2011
Reviewer: Sang Woon Choi
Reviewer's report:
Major Compulsory Revisions
1) Page 2: The method section of the abstract needs to be changed. Authors just
listed the names of method and did not address what they measured.
2) Page 12, Line 4: ‘IGF-1 stimulated the association of the p58 regulatory….’
What is ‘the association’? Please make it clear.
3) The conclusion section includes only a brief summary of the results. The
statement of significance of the results or the future perspectives could be
needed.
Minor Essential Revisions
1) Page 2: The method section of the abstract: What does MTT stands for? If the
method is just a routine one for cell culture work, it doesn’t need to be here.
Discretionary Revisions
1) Does HT-29 cell also increase the synthesis of IGF-I? If so, can it also be
inhibited by luteolin?
2) Would it be reasonable to test if IGF II stimulated HT-29 cell growth also does
not abrogate the luteolin-induced growth inhibition?
3) Authors used only one cancer cell line. It is curious whether or not luteolin also
inhibits normal cell’s IGF-IR signaling. If so, too much luteolin could be bad for
the normal cells, even though it is good to inhibit the cancer cell growth?
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
assistant