Reviewer's report
Title:Small intestinal mucosa expression of putative chaperone fls485
Version: 1Date:13 July 2009
Reviewer: Dan Hershko
Reviewer's report:
In the present manuscript, the authors describe the expression of fls485 in the
small mucosa of normal subjects and patients wuth celiac disease. this study
was followed previous findings by the group that the fls485 gene was 1 among
415 genes expressed in enterocytes. The expression and function of here they
show that fls485 protein in human tissues is unknown.
The authors have found, as previously reported, that fls485 is found with
increasing gradient from crypt to villi of normal mucosa and that the expression of
the protein is impared in celiac disease Marsh IIIa-c.
Major concerns:
1. the antibodies generated expressed two distinct bands, one of the expected
size and another unexpected band. This may lead to false interpretation of the
results observed by immunohistochemistry.
2. although the authors claim that an agreement between mRNA exists, they do
not provide the results of all experiments and the distribution of the results in
normal mucosa. attempts should also be made to reproduce the results of in-situ
hybridization.
3. Since the function and meaning of these results are still unclear the
importance of the findings are of limited interest.
Level of interest: An article of limited interest
Quality of written English: Needs some language corrections before being
published
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
'I declare that I have no competing interests'
Reviewer's report
Title:Small intestinal mucosa expression of putative chaperone fls485
Version: 1Date:13 July 2009
Reviewer: Dan Hershko
Reviewer's report:
In the present manuscript, the authors describe the expression of fls485 in the
small mucosa of normal subjects and patients wuth celiac disease. this study
was followed previous findings by the group that the fls485 gene was 1 among
415 genes expressed in enterocytes. The expression and function of here they
show that fls485 protein in human tissues is unknown.
The authors have found, as previously reported, that fls485 is found with
increasing gradient from crypt to villi of normal mucosa and that the expression of
the protein is impared in celiac disease Marsh IIIa-c.
Major concerns:
1. the antibodies generated expressed two distinct bands, one of the expected
size and another unexpected band. This may lead to false interpretation of the
results observed by immunohistochemistry.
2. although the authors claim that an agreement between mRNA exists, they do
not provide the results of all experiments and the distribution of the results in
normal mucosa. attempts should also be made to reproduce the results of in-situ
hybridization.
3. Since the function and meaning of these results are still unclear the
importance of the findings are of limited interest.
Level of interest: An article of limited interest
Quality of written English: Needs some language corrections before being
published
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
'I declare that I have no competing interests'
Please review this paper.
user
Reviewer's report
Title:Small intestinal mucosa expression of putative chaperone fls485
Version: 1Date:13 July 2009
Reviewer: Dan Hershko
Reviewer's report:
In the present manuscript, the authors describe the expression of fls485 in the
small mucosa of normal subjects and patients wuth celiac disease. this study
was followed previous findings by the group that the fls485 gene was 1 among
415 genes expressed in enterocytes. The expression and function of here they
show that fls485 protein in human tissues is unknown.
The authors have found, as previously reported, that fls485 is found with
increasing gradient from crypt to villi of normal mucosa and that the expression of
the protein is impared in celiac disease Marsh IIIa-c.
Major concerns:
1. the antibodies generated expressed two distinct bands, one of the expected
size and another unexpected band. This may lead to false interpretation of the
results observed by immunohistochemistry.
2. although the authors claim that an agreement between mRNA exists, they do
not provide the results of all experiments and the distribution of the results in
normal mucosa. attempts should also be made to reproduce the results of in-situ
hybridization.
3. Since the function and meaning of these results are still unclear the
importance of the findings are of limited interest.
Level of interest: An article of limited interest
Quality of written English: Needs some language corrections before being
published
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
'I declare that I have no competing interests'
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