Reviewer's report
Title:Up-Regulation of CNDP2 Facilitates the Proliferation of Colon Cancer
Version: 1Date:15 September 2013
Reviewer: Katarzyna Kilis-Pstrusinska
Reviewer's report:
The Authors studied the possible role of CNDP2 in colon cancer proliferation.
They applied two different approaches. Firstly, they analysed CNDP2 expression
in colon cancers and its relationship with clinico-pathological characteristics of
patients. Secondly, they studied the CNDP2 association with cell proliferation,
cell cycle progression and tumorigenicity.
The paper deserves attention. However, The Authors should improve it.
Major Compulsory Revisions
Introduction.
1. CNDP2, cytosol non-specific dipeptidase, is a member of the M20 family of
metalloproteases. The CNDP2 gene encodes CNDP2, not „ CNDP2 gene
belongs to the M20 family”. This sentence should be corrected.
2. The last sentences are unclear. Did the Authors publish the papers concerning
CNDP2 and colon cancer previously? The Authors wrote: “The strong CNDP2
expression that we observed in neoplastic colon cells and tumor tissues
suggested a potential role for CNDP2 in colon tumorigenesis. Knockdown of
CNDP2 reduced tumor cell growth, colony formation and inhibited colon cancer
cell (RKO) tumorigenicity in nude mice. Our work demonstrates that CNDP2
promotes cancer growth. Moreover, CNDP2 plays a significant role in different
colon locations”. If the results are from the current study, above sentences
should be given in the part Discussion.
Material and Methods
1. Lack the information concerning samples of colon mucosa and benign colon
diseases (BCD). What kind of BCD had the patients whose samples were
examined?
2. Please add the reference to TNM stages of colorectal cancer defined by the
WHO.
Discussion. Conclusions.
1. The limitations of the work aren’t stated.
2. EGFR, cyclin B1 and cyclin E were not a subject of the study. The Authors
analysed these proteins expression only for CNDP2. Therefore, the sentence in
part Conclusions “ EGFR, cyclin B1 (…) is unfounded and not the result of
current study.
Minor Essential Revisions
Abstract
The Authors wrote: „CNDP2 expression was markedly increased in clinical colon
tumors and colon cancer cell lines.” Compared with?
Table
Table 1: There are some mistakes, e.g. variales (variables?). CNDP2 expression
in the title and Kin17 expression in body of table. It is unclear.
The abbreviations used in the table 1 should be explained below it.
Figures
Figure legends contain legend for Figure 1-3 and S1-S2. There are figures 1 to 5
in the paper. These discrepancies should be corrected.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:Up-Regulation of CNDP2 Facilitates the Proliferation of Colon Cancer
Version: 1Date:15 September 2013
Reviewer: Katarzyna Kilis-Pstrusinska
Reviewer's report:
The Authors studied the possible role of CNDP2 in colon cancer proliferation.
They applied two different approaches. Firstly, they analysed CNDP2 expression
in colon cancers and its relationship with clinico-pathological characteristics of
patients. Secondly, they studied the CNDP2 association with cell proliferation,
cell cycle progression and tumorigenicity.
The paper deserves attention. However, The Authors should improve it.
Major Compulsory Revisions
Introduction.
1. CNDP2, cytosol non-specific dipeptidase, is a member of the M20 family of
metalloproteases. The CNDP2 gene encodes CNDP2, not „ CNDP2 gene
belongs to the M20 family”. This sentence should be corrected.
2. The last sentences are unclear. Did the Authors publish the papers concerning
CNDP2 and colon cancer previously? The Authors wrote: “The strong CNDP2
expression that we observed in neoplastic colon cells and tumor tissues
suggested a potential role for CNDP2 in colon tumorigenesis. Knockdown of
CNDP2 reduced tumor cell growth, colony formation and inhibited colon cancer
cell (RKO) tumorigenicity in nude mice. Our work demonstrates that CNDP2
promotes cancer growth. Moreover, CNDP2 plays a significant role in different
colon locations”. If the results are from the current study, above sentences
should be given in the part Discussion.
Material and Methods
1. Lack the information concerning samples of colon mucosa and benign colon
diseases (BCD). What kind of BCD had the patients whose samples were
examined?
2. Please add the reference to TNM stages of colorectal cancer defined by the
WHO.
Discussion. Conclusions.
1. The limitations of the work aren’t stated.
2. EGFR, cyclin B1 and cyclin E were not a subject of the study. The Authors
analysed these proteins expression only for CNDP2. Therefore, the sentence in
part Conclusions “ EGFR, cyclin B1 (…) is unfounded and not the result of
current study.
Minor Essential Revisions
Abstract
The Authors wrote: „CNDP2 expression was markedly increased in clinical colon
tumors and colon cancer cell lines.” Compared with?
Table
Table 1: There are some mistakes, e.g. variales (variables?). CNDP2 expression
in the title and Kin17 expression in body of table. It is unclear.
The abbreviations used in the table 1 should be explained below it.
Figures
Figure legends contain legend for Figure 1-3 and S1-S2. There are figures 1 to 5
in the paper. These discrepancies should be corrected.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:Up-Regulation of CNDP2 Facilitates the Proliferation of Colon Cancer
Version: 1Date:15 September 2013
Reviewer: Katarzyna Kilis-Pstrusinska
Reviewer's report:
The Authors studied the possible role of CNDP2 in colon cancer proliferation.
They applied two different approaches. Firstly, they analysed CNDP2 expression
in colon cancers and its relationship with clinico-pathological characteristics of
patients. Secondly, they studied the CNDP2 association with cell proliferation,
cell cycle progression and tumorigenicity.
The paper deserves attention. However, The Authors should improve it.
Major Compulsory Revisions
Introduction.
1. CNDP2, cytosol non-specific dipeptidase, is a member of the M20 family of
metalloproteases. The CNDP2 gene encodes CNDP2, not „ CNDP2 gene
belongs to the M20 family”. This sentence should be corrected.
2. The last sentences are unclear. Did the Authors publish the papers concerning
CNDP2 and colon cancer previously? The Authors wrote: “The strong CNDP2
expression that we observed in neoplastic colon cells and tumor tissues
suggested a potential role for CNDP2 in colon tumorigenesis. Knockdown of
CNDP2 reduced tumor cell growth, colony formation and inhibited colon cancer
cell (RKO) tumorigenicity in nude mice. Our work demonstrates that CNDP2
promotes cancer growth. Moreover, CNDP2 plays a significant role in different
colon locations”. If the results are from the current study, above sentences
should be given in the part Discussion.
Material and Methods
1. Lack the information concerning samples of colon mucosa and benign colon
diseases (BCD). What kind of BCD had the patients whose samples were
examined?
2. Please add the reference to TNM stages of colorectal cancer defined by the
WHO.
Discussion. Conclusions.
1. The limitations of the work aren’t stated.
2. EGFR, cyclin B1 and cyclin E were not a subject of the study. The Authors
analysed these proteins expression only for CNDP2. Therefore, the sentence in
part Conclusions “ EGFR, cyclin B1 (…) is unfounded and not the result of
current study.
Minor Essential Revisions
Abstract
The Authors wrote: „CNDP2 expression was markedly increased in clinical colon
tumors and colon cancer cell lines.” Compared with?
Table
Table 1: There are some mistakes, e.g. variales (variables?). CNDP2 expression
in the title and Kin17 expression in body of table. It is unclear.
The abbreviations used in the table 1 should be explained below it.
Figures
Figure legends contain legend for Figure 1-3 and S1-S2. There are figures 1 to 5
in the paper. These discrepancies should be corrected.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
assistant