Reviewer's report
Title:Association of Gut Microbiota with Post-operative Clinical Course in
Crohn's Disease
Version: 2Date:11 April 2013
Reviewer: arsun swaminath
Reviewer's report:
Dr Dey et al. have provided pilot data to characterize changes to the microbiome
in a small groups of patients with Crohn’s disease who have undergone surgery.
Minor Comments:
1) If these patients were not specifically recruited for this study, was the tissue
obtained from a tissue bank? Was each colonoscopy done at 6mo post op or
was there
2) Page 3 states that colonoscopy was done for “check up.” This should be
worked to explain study was done per standard clinical protocol to assess
disease recurrence at a specific time point post operatively.
3) A significant different in the types of species identified is found on pg 13, but
refers to a supplemental figure, which refers back to fig 1c#maybe more useful to
integrate fig S2 into Fig 1.
Major Comments:
1) No table of clinical information is provided. If there are a limited number of
patients, it would be useful to give the reader an idea of how heterogenous the
studied population is given all the variables that can affect the microbiome
-include duration of disease,disease phenotype, distribution, pre-op meds and
post-op meds (esp if antibiotics were part of the post op regimen), time of post-op
colonoscopy
2) If the same biopsy forcep is used through the same colonoscopy channel to
biopsy different sites within the same patients, does that allow you to answer the
question you’re interested in? Are you actually able to distinguish between
inflamed and uninflamed sites within the same patient? This information is not
included in fig 1b # inflamed vs noninflamed within patient.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Reviewer's report
Title:Association of Gut Microbiota with Post-operative Clinical Course in
Crohn's Disease
Version: 2Date:11 April 2013
Reviewer: arsun swaminath
Reviewer's report:
Dr Dey et al. have provided pilot data to characterize changes to the microbiome
in a small groups of patients with Crohn’s disease who have undergone surgery.
Minor Comments:
1) If these patients were not specifically recruited for this study, was the tissue
obtained from a tissue bank? Was each colonoscopy done at 6mo post op or
was there
2) Page 3 states that colonoscopy was done for “check up.” This should be
worked to explain study was done per standard clinical protocol to assess
disease recurrence at a specific time point post operatively.
3) A significant different in the types of species identified is found on pg 13, but
refers to a supplemental figure, which refers back to fig 1c#maybe more useful to
integrate fig S2 into Fig 1.
Major Comments:
1) No table of clinical information is provided. If there are a limited number of
patients, it would be useful to give the reader an idea of how heterogenous the
studied population is given all the variables that can affect the microbiome
-include duration of disease,disease phenotype, distribution, pre-op meds and
post-op meds (esp if antibiotics were part of the post op regimen), time of post-op
colonoscopy
2) If the same biopsy forcep is used through the same colonoscopy channel to
biopsy different sites within the same patients, does that allow you to answer the
question you’re interested in? Are you actually able to distinguish between
inflamed and uninflamed sites within the same patient? This information is not
included in fig 1b # inflamed vs noninflamed within patient.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Please review this paper.
user
Reviewer's report
Title:Association of Gut Microbiota with Post-operative Clinical Course in
Crohn's Disease
Version: 2Date:11 April 2013
Reviewer: arsun swaminath
Reviewer's report:
Dr Dey et al. have provided pilot data to characterize changes to the microbiome
in a small groups of patients with Crohn’s disease who have undergone surgery.
Minor Comments:
1) If these patients were not specifically recruited for this study, was the tissue
obtained from a tissue bank? Was each colonoscopy done at 6mo post op or
was there
2) Page 3 states that colonoscopy was done for “check up.” This should be
worked to explain study was done per standard clinical protocol to assess
disease recurrence at a specific time point post operatively.
3) A significant different in the types of species identified is found on pg 13, but
refers to a supplemental figure, which refers back to fig 1c#maybe more useful to
integrate fig S2 into Fig 1.
Major Comments:
1) No table of clinical information is provided. If there are a limited number of
patients, it would be useful to give the reader an idea of how heterogenous the
studied population is given all the variables that can affect the microbiome
-include duration of disease,disease phenotype, distribution, pre-op meds and
post-op meds (esp if antibiotics were part of the post op regimen), time of post-op
colonoscopy
2) If the same biopsy forcep is used through the same colonoscopy channel to
biopsy different sites within the same patients, does that allow you to answer the
question you’re interested in? Are you actually able to distinguish between
inflamed and uninflamed sites within the same patient? This information is not
included in fig 1b # inflamed vs noninflamed within patient.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
assistant