Reviewer's report
Title:Palm Vitamin E Reduces Catecholamines, Xanthine Oxidase Activity and
Gastric Lesions in Rats Exposed To Water-Immersion Restraint Stress
Version: 1Date:22 November 2011
Reviewer: Fang Yan
Reviewer's report:
In this manuscript, the authors studied effects of Palm vitamin E (PVE) and
#-tocopherol (#-TF) supplementation on gastric lesions in rats exposed to
water-immersion restraint
stress. The results from these experiments showed that supplementations with
either PVE or #-TF reduced gastric lesions, inhibited stress induced elevation of
adrenalin and noradrenalin in the blood and decreased xanthine oxidase and
dehydrogenase activities in the stomach.
Major revisions;
1. All of these effects were detected immediately after rats were exposed to
stress. Does the stress induce sever gastric injury at later time point, compared
to that occurring immediately after injury? The authors need to test the effects of
PVE and #-TF on gastric injury in more than one time point.
2. A brief explanation method should be added to the figure legend, which will
help the readers to understand the data from these experiments.
3. Histology of gastric tissues should be assessed to address the gastric injury.
4.Other cellular responses in the gastric tissues that are related to decreased
gastric injury should be detected to support the protective effects of PVE and
#-TF.
5. PVE and #-TF may have direct protective roles in the gastric tissue. What is
the mechanism of reduction of adrenalin and noradrenalin in blood by PVE and
#-TF?
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:Palm Vitamin E Reduces Catecholamines, Xanthine Oxidase Activity and
Gastric Lesions in Rats Exposed To Water-Immersion Restraint Stress
Version: 1Date:22 November 2011
Reviewer: Fang Yan
Reviewer's report:
In this manuscript, the authors studied effects of Palm vitamin E (PVE) and
#-tocopherol (#-TF) supplementation on gastric lesions in rats exposed to
water-immersion restraint
stress. The results from these experiments showed that supplementations with
either PVE or #-TF reduced gastric lesions, inhibited stress induced elevation of
adrenalin and noradrenalin in the blood and decreased xanthine oxidase and
dehydrogenase activities in the stomach.
Major revisions;
1. All of these effects were detected immediately after rats were exposed to
stress. Does the stress induce sever gastric injury at later time point, compared
to that occurring immediately after injury? The authors need to test the effects of
PVE and #-TF on gastric injury in more than one time point.
2. A brief explanation method should be added to the figure legend, which will
help the readers to understand the data from these experiments.
3. Histology of gastric tissues should be assessed to address the gastric injury.
4.Other cellular responses in the gastric tissues that are related to decreased
gastric injury should be detected to support the protective effects of PVE and
#-TF.
5. PVE and #-TF may have direct protective roles in the gastric tissue. What is
the mechanism of reduction of adrenalin and noradrenalin in blood by PVE and
#-TF?
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:Palm Vitamin E Reduces Catecholamines, Xanthine Oxidase Activity and
Gastric Lesions in Rats Exposed To Water-Immersion Restraint Stress
Version: 1Date:22 November 2011
Reviewer: Fang Yan
Reviewer's report:
In this manuscript, the authors studied effects of Palm vitamin E (PVE) and
#-tocopherol (#-TF) supplementation on gastric lesions in rats exposed to
water-immersion restraint
stress. The results from these experiments showed that supplementations with
either PVE or #-TF reduced gastric lesions, inhibited stress induced elevation of
adrenalin and noradrenalin in the blood and decreased xanthine oxidase and
dehydrogenase activities in the stomach.
Major revisions;
1. All of these effects were detected immediately after rats were exposed to
stress. Does the stress induce sever gastric injury at later time point, compared
to that occurring immediately after injury? The authors need to test the effects of
PVE and #-TF on gastric injury in more than one time point.
2. A brief explanation method should be added to the figure legend, which will
help the readers to understand the data from these experiments.
3. Histology of gastric tissues should be assessed to address the gastric injury.
4.Other cellular responses in the gastric tissues that are related to decreased
gastric injury should be detected to support the protective effects of PVE and
#-TF.
5. PVE and #-TF may have direct protective roles in the gastric tissue. What is
the mechanism of reduction of adrenalin and noradrenalin in blood by PVE and
#-TF?
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
Declaration of competing interests:
I declare that I have no competing interests.
assistant