Title: Rhaponticin suppresses the stemness phenotype of gastric cancer stem-like cells CD133+/
CD166+ by inhibiting programmed death-ligand 1
Version: 2 Date: 26 Jul 2024
Reviewer's report:
# In Fig1, are the population of CD133+/CD166+ cells lower? Please add double stained data
of parent BGC-823 and SGC 7901 cells.
# In Fig4, Did Rhaponticin reduce CD133+/CD166+ cells? In addition, does PD-L1 knockdown
influence CD133+ and/or CD166+ expression? Please add further data and discussion.
#In Fig5, generally, nude mice are deficient in the thymus gland, so T cells are almost non-
existent. Please explain with data why CD4+ or CD8+ cells are present.
#Please add further data and discussion on the mechanism of action of Rhaponticin to reduce
PD-L1 expression.
Title: Rhaponticin suppresses the stemness phenotype of gastric cancer stem-like cells CD133+/
CD166+ by inhibiting programmed death-ligand 1
Version: 2 Date: 26 Jul 2024
Reviewer's report:
# In Fig1, are the population of CD133+/CD166+ cells lower? Please add double stained data
of parent BGC-823 and SGC 7901 cells.
# In Fig4, Did Rhaponticin reduce CD133+/CD166+ cells? In addition, does PD-L1 knockdown
influence CD133+ and/or CD166+ expression? Please add further data and discussion.
#In Fig5, generally, nude mice are deficient in the thymus gland, so T cells are almost non-
existent. Please explain with data why CD4+ or CD8+ cells are present.
#Please add further data and discussion on the mechanism of action of Rhaponticin to reduce
PD-L1 expression.
Please review this paper.
user
Title: Rhaponticin suppresses the stemness phenotype of gastric cancer stem-like cells CD133+/
CD166+ by inhibiting programmed death-ligand 1
Version: 2 Date: 26 Jul 2024
Reviewer's report:
# In Fig1, are the population of CD133+/CD166+ cells lower? Please add double stained data
of parent BGC-823 and SGC 7901 cells.
# In Fig4, Did Rhaponticin reduce CD133+/CD166+ cells? In addition, does PD-L1 knockdown
influence CD133+ and/or CD166+ expression? Please add further data and discussion.
#In Fig5, generally, nude mice are deficient in the thymus gland, so T cells are almost non-
existent. Please explain with data why CD4+ or CD8+ cells are present.
#Please add further data and discussion on the mechanism of action of Rhaponticin to reduce
PD-L1 expression.
assistant