Reviewer's report
Title:Mycoplasma hyorhinis infection in gastric carcinoma and its effects on the
malignant phenotypes of gastric cancer cells
Version: 1Date:9 August 2010
Reviewer: zheng hua-chuan
Reviewer's report:
Minor essential revisions
In the manuscript entitled " Mycoplasma hyorhinis infection in gastric carcinoma
and its effects on the malignant phenotypes of gastric cancer cells“, Yang et al.
reported that the existence of Mycoplasma hyorhinis was positively correlated
with lymph node metastasis, Lauren’s classification, TNM staging and patient
age of gastric cancer. Mycoplasma hyorhinis promoted tumor cell migration,
invasion and metastasis, which was possibly associated with the enhanced
phosphorylation of EGFR and ERK1/2, while the antibody against p37 protein
could block these biological events, mentioned above. Overall, the design of the
manuscript is very nice and of greatly scientific significance although the current
manuscript suffers from no novel findings and numerous grammatical and syntax
errors.
Minor comments:
1. It is very rare to say “Lauren Typing” in stead of “Lauren’s classification”.
2. Please give the full name when abbreviation for the first time appears.
3. In Page 5 line 2, please tell how to process the gastric cancer tissues.
4. In nested PCR, what amount of DNA has been added in PCR mixture?
5. The description bout the second set of PCR primers is confusing for
Mycoplasma hyorhinis examination. What about the first set?
6. It is better to give a brief description about why to use the mouse B16f10 cell
for the metastatic experiments of C57B/6.
7. What about the Mycoplasma hyorhinis existence in non-neoplastic mucosa
adjacent to gastric carcinoma.
8. The positive rate of Mycoplasma hyorhinis existence was a little higher by
nested-PCR than immunohistochemistry(IHC). However, sensitivity of
nested-PCR is much higher using 10-fold dilution of the first PCR product.
Therefore, the data of IHC should be confirmed by Western blot if possible.
9. Please give the statistical description in the figures and their legends.
10. In page 13 line 7, have you analyzed the association between Mycoplasma
hyorhinis existence and prognosis of gastric carcinoma patients?
11. There were many mistakes in spelling, syntax and grammar throughout the
manuscript. For example, “we……” is frequently used in the paper. It is
necessary to revise the manuscript by a native English-speaker.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Reviewer's report
Title:Mycoplasma hyorhinis infection in gastric carcinoma and its effects on the
malignant phenotypes of gastric cancer cells
Version: 1Date:9 August 2010
Reviewer: zheng hua-chuan
Reviewer's report:
Minor essential revisions
In the manuscript entitled " Mycoplasma hyorhinis infection in gastric carcinoma
and its effects on the malignant phenotypes of gastric cancer cells“, Yang et al.
reported that the existence of Mycoplasma hyorhinis was positively correlated
with lymph node metastasis, Lauren’s classification, TNM staging and patient
age of gastric cancer. Mycoplasma hyorhinis promoted tumor cell migration,
invasion and metastasis, which was possibly associated with the enhanced
phosphorylation of EGFR and ERK1/2, while the antibody against p37 protein
could block these biological events, mentioned above. Overall, the design of the
manuscript is very nice and of greatly scientific significance although the current
manuscript suffers from no novel findings and numerous grammatical and syntax
errors.
Minor comments:
1. It is very rare to say “Lauren Typing” in stead of “Lauren’s classification”.
2. Please give the full name when abbreviation for the first time appears.
3. In Page 5 line 2, please tell how to process the gastric cancer tissues.
4. In nested PCR, what amount of DNA has been added in PCR mixture?
5. The description bout the second set of PCR primers is confusing for
Mycoplasma hyorhinis examination. What about the first set?
6. It is better to give a brief description about why to use the mouse B16f10 cell
for the metastatic experiments of C57B/6.
7. What about the Mycoplasma hyorhinis existence in non-neoplastic mucosa
adjacent to gastric carcinoma.
8. The positive rate of Mycoplasma hyorhinis existence was a little higher by
nested-PCR than immunohistochemistry(IHC). However, sensitivity of
nested-PCR is much higher using 10-fold dilution of the first PCR product.
Therefore, the data of IHC should be confirmed by Western blot if possible.
9. Please give the statistical description in the figures and their legends.
10. In page 13 line 7, have you analyzed the association between Mycoplasma
hyorhinis existence and prognosis of gastric carcinoma patients?
11. There were many mistakes in spelling, syntax and grammar throughout the
manuscript. For example, “we……” is frequently used in the paper. It is
necessary to revise the manuscript by a native English-speaker.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Please review this paper.
user
Reviewer's report
Title:Mycoplasma hyorhinis infection in gastric carcinoma and its effects on the
malignant phenotypes of gastric cancer cells
Version: 1Date:9 August 2010
Reviewer: zheng hua-chuan
Reviewer's report:
Minor essential revisions
In the manuscript entitled " Mycoplasma hyorhinis infection in gastric carcinoma
and its effects on the malignant phenotypes of gastric cancer cells“, Yang et al.
reported that the existence of Mycoplasma hyorhinis was positively correlated
with lymph node metastasis, Lauren’s classification, TNM staging and patient
age of gastric cancer. Mycoplasma hyorhinis promoted tumor cell migration,
invasion and metastasis, which was possibly associated with the enhanced
phosphorylation of EGFR and ERK1/2, while the antibody against p37 protein
could block these biological events, mentioned above. Overall, the design of the
manuscript is very nice and of greatly scientific significance although the current
manuscript suffers from no novel findings and numerous grammatical and syntax
errors.
Minor comments:
1. It is very rare to say “Lauren Typing” in stead of “Lauren’s classification”.
2. Please give the full name when abbreviation for the first time appears.
3. In Page 5 line 2, please tell how to process the gastric cancer tissues.
4. In nested PCR, what amount of DNA has been added in PCR mixture?
5. The description bout the second set of PCR primers is confusing for
Mycoplasma hyorhinis examination. What about the first set?
6. It is better to give a brief description about why to use the mouse B16f10 cell
for the metastatic experiments of C57B/6.
7. What about the Mycoplasma hyorhinis existence in non-neoplastic mucosa
adjacent to gastric carcinoma.
8. The positive rate of Mycoplasma hyorhinis existence was a little higher by
nested-PCR than immunohistochemistry(IHC). However, sensitivity of
nested-PCR is much higher using 10-fold dilution of the first PCR product.
Therefore, the data of IHC should be confirmed by Western blot if possible.
9. Please give the statistical description in the figures and their legends.
10. In page 13 line 7, have you analyzed the association between Mycoplasma
hyorhinis existence and prognosis of gastric carcinoma patients?
11. There were many mistakes in spelling, syntax and grammar throughout the
manuscript. For example, “we……” is frequently used in the paper. It is
necessary to revise the manuscript by a native English-speaker.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
assistant