Title: The gut microbiota composition in patients with right- and left-sided colorectal cancer
and after curative colectomy, as analyzed by 16S rRNA gene amplicon sequencing
Version: 3 Date: 15 Mar 2022
Reviewer's report:
This manuscript is well conducted, however I think that some aspects need to be clarified and
the quality needs to be improved in order to be considered for publication.
- The authors should clarify why they used two methodologies to determine the composition of
the microbiota. If T-RFLP was used as a filter to reduce the number of samples to be analysed
by NGS, what were the sample selection criteria?
In addition, the title of the manuscript states that the analysis of the microbiota is based on NGS.
- Keep the term microbiota throughout the article, in some sections you may see the term "flora".
- Please check carefully for grammatical errors and typos during proofreading.
-Page 10, “The LDA score is useful in discriminating between each group”, this sentence needs
to be supported with more information, on what basis is this assertion made?
-Page 11, subtitle: “Metagenomic analysis” needs to be revised, this section describes the
prediction of metabolic potential. The same applies to the titles of tables 3 and 4.
Title: The gut microbiota composition in patients with right- and left-sided colorectal cancer
and after curative colectomy, as analyzed by 16S rRNA gene amplicon sequencing
Version: 3 Date: 15 Mar 2022
Reviewer's report:
This manuscript is well conducted, however I think that some aspects need to be clarified and
the quality needs to be improved in order to be considered for publication.
- The authors should clarify why they used two methodologies to determine the composition of
the microbiota. If T-RFLP was used as a filter to reduce the number of samples to be analysed
by NGS, what were the sample selection criteria?
In addition, the title of the manuscript states that the analysis of the microbiota is based on NGS.
- Keep the term microbiota throughout the article, in some sections you may see the term "flora".
- Please check carefully for grammatical errors and typos during proofreading.
-Page 10, “The LDA score is useful in discriminating between each group”, this sentence needs
to be supported with more information, on what basis is this assertion made?
-Page 11, subtitle: “Metagenomic analysis” needs to be revised, this section describes the
prediction of metabolic potential. The same applies to the titles of tables 3 and 4.
Please review this paper.
user
Title: The gut microbiota composition in patients with right- and left-sided colorectal cancer
and after curative colectomy, as analyzed by 16S rRNA gene amplicon sequencing
Version: 3 Date: 15 Mar 2022
Reviewer's report:
This manuscript is well conducted, however I think that some aspects need to be clarified and
the quality needs to be improved in order to be considered for publication.
- The authors should clarify why they used two methodologies to determine the composition of
the microbiota. If T-RFLP was used as a filter to reduce the number of samples to be analysed
by NGS, what were the sample selection criteria?
In addition, the title of the manuscript states that the analysis of the microbiota is based on NGS.
- Keep the term microbiota throughout the article, in some sections you may see the term "flora".
- Please check carefully for grammatical errors and typos during proofreading.
-Page 10, “The LDA score is useful in discriminating between each group”, this sentence needs
to be supported with more information, on what basis is this assertion made?
-Page 11, subtitle: “Metagenomic analysis” needs to be revised, this section describes the
prediction of metabolic potential. The same applies to the titles of tables 3 and 4.
assistant