Reviewer's report
Title:Fatty acid composition of subcutaneous adipose tissue and gastric
mucosa: is there a relation with gastric ulceration?
Version: 1Date:8 July 2008
Reviewer: Stachowska Ewa
Reviewer's report:
Article : Fatty acid composition of subcutaneous adipose tissue and gastric
mucosa : is there an relation with gastric ucleration?
VA Packgalos et al.
1. Is the question posed by the authors well defined? – Yes
2. Are the methods appropriate and well described? – No
3. Are the data sound? -Yes
4. Does the manuscript adhere to the relevant standards for reporting and data
deposition? - No
5. Are the discussion and conclusions well balanced and adequately supported
by the data?- Yes
6. Are limitations of the work clearly stated?- Yes
7. Do the authors clearly acknowledge any work upon which they are building,
both published and unpublished?
8. Do the title and abstract accurately convey what has been found? - Yes
9. Is the writing acceptable? -Yes
Please make your review as constructive and detailed as possible in your
comments so that
- Discretionary Revisions (which are recommendations for improvement but
which the author can choose to ignore)
- Minor Essential Revisions (such as missing labels on figures, or the wrong use
of a term, which the author can be trusted to correct)
- Major Compulsory Revisions (which the author must respond to before a
decision on publication can be reached)Background
Sugestion:
1. Page 3 .
It is not true that PUFA cannot be endogenously syntesized from carbohydrates-
see charter „Biosynthesis of Fatty acids” from Harper’s Biochemistry (RK Murray
et al.). In this way oleic acid - precursor of n-9 family should belong to an
essential fatty acids (EFA) – as we know, it isn’t true. Only two EFA: linoleic and
alpha linolenic can be use as biomarkers of dietary intake.
2. Page 4 – from linoleic acid - two different of eikosconids precursor (20 carbon
fatty acids) are synthesized:
a)dihommogamma linoleic – that are of precursor of prostanoids (prostaglandins
and tromboxanes) from series 1 and leukotriens and lipoxins from series 3
b) arachidonic amid tahat are precursor of prostaglandins and TX from 2 series
and LT from 4 series.
PGs (eg PGE2) that are synthesized from arachidonic acid, have both - anti
inflammatory and proinflammatory function. It is a possible to all this PG, always
are able to protect against noxius agents?
3. Page 5 and 6
What was a reason of application of two columns and conditions of fatty acids
separation from different tissues?
4. Page 8 and 9 - That are incomprehensible recordings.- Is C 14:19 cis - mean
C14 : 1cis-9? Or what is mean- C14:19,12c? or C 20 :01?
Level of interest: An article of limited interest
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
Reviewer's report
Title:Fatty acid composition of subcutaneous adipose tissue and gastric
mucosa: is there a relation with gastric ulceration?
Version: 1Date:8 July 2008
Reviewer: Stachowska Ewa
Reviewer's report:
Article : Fatty acid composition of subcutaneous adipose tissue and gastric
mucosa : is there an relation with gastric ucleration?
VA Packgalos et al.
1. Is the question posed by the authors well defined? – Yes
2. Are the methods appropriate and well described? – No
3. Are the data sound? -Yes
4. Does the manuscript adhere to the relevant standards for reporting and data
deposition? - No
5. Are the discussion and conclusions well balanced and adequately supported
by the data?- Yes
6. Are limitations of the work clearly stated?- Yes
7. Do the authors clearly acknowledge any work upon which they are building,
both published and unpublished?
8. Do the title and abstract accurately convey what has been found? - Yes
9. Is the writing acceptable? -Yes
Please make your review as constructive and detailed as possible in your
comments so that
- Discretionary Revisions (which are recommendations for improvement but
which the author can choose to ignore)
- Minor Essential Revisions (such as missing labels on figures, or the wrong use
of a term, which the author can be trusted to correct)
- Major Compulsory Revisions (which the author must respond to before a
decision on publication can be reached)Background
Sugestion:
1. Page 3 .
It is not true that PUFA cannot be endogenously syntesized from carbohydrates-
see charter „Biosynthesis of Fatty acids” from Harper’s Biochemistry (RK Murray
et al.). In this way oleic acid - precursor of n-9 family should belong to an
essential fatty acids (EFA) – as we know, it isn’t true. Only two EFA: linoleic and
alpha linolenic can be use as biomarkers of dietary intake.
2. Page 4 – from linoleic acid - two different of eikosconids precursor (20 carbon
fatty acids) are synthesized:
a)dihommogamma linoleic – that are of precursor of prostanoids (prostaglandins
and tromboxanes) from series 1 and leukotriens and lipoxins from series 3
b) arachidonic amid tahat are precursor of prostaglandins and TX from 2 series
and LT from 4 series.
PGs (eg PGE2) that are synthesized from arachidonic acid, have both - anti
inflammatory and proinflammatory function. It is a possible to all this PG, always
are able to protect against noxius agents?
3. Page 5 and 6
What was a reason of application of two columns and conditions of fatty acids
separation from different tissues?
4. Page 8 and 9 - That are incomprehensible recordings.- Is C 14:19 cis - mean
C14 : 1cis-9? Or what is mean- C14:19,12c? or C 20 :01?
Level of interest: An article of limited interest
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
Please review this paper.
user
Reviewer's report
Title:Fatty acid composition of subcutaneous adipose tissue and gastric
mucosa: is there a relation with gastric ulceration?
Version: 1Date:8 July 2008
Reviewer: Stachowska Ewa
Reviewer's report:
Article : Fatty acid composition of subcutaneous adipose tissue and gastric
mucosa : is there an relation with gastric ucleration?
VA Packgalos et al.
1. Is the question posed by the authors well defined? – Yes
2. Are the methods appropriate and well described? – No
3. Are the data sound? -Yes
4. Does the manuscript adhere to the relevant standards for reporting and data
deposition? - No
5. Are the discussion and conclusions well balanced and adequately supported
by the data?- Yes
6. Are limitations of the work clearly stated?- Yes
7. Do the authors clearly acknowledge any work upon which they are building,
both published and unpublished?
8. Do the title and abstract accurately convey what has been found? - Yes
9. Is the writing acceptable? -Yes
Please make your review as constructive and detailed as possible in your
comments so that
- Discretionary Revisions (which are recommendations for improvement but
which the author can choose to ignore)
- Minor Essential Revisions (such as missing labels on figures, or the wrong use
of a term, which the author can be trusted to correct)
- Major Compulsory Revisions (which the author must respond to before a
decision on publication can be reached)Background
Sugestion:
1. Page 3 .
It is not true that PUFA cannot be endogenously syntesized from carbohydrates-
see charter „Biosynthesis of Fatty acids” from Harper’s Biochemistry (RK Murray
et al.). In this way oleic acid - precursor of n-9 family should belong to an
essential fatty acids (EFA) – as we know, it isn’t true. Only two EFA: linoleic and
alpha linolenic can be use as biomarkers of dietary intake.
2. Page 4 – from linoleic acid - two different of eikosconids precursor (20 carbon
fatty acids) are synthesized:
a)dihommogamma linoleic – that are of precursor of prostanoids (prostaglandins
and tromboxanes) from series 1 and leukotriens and lipoxins from series 3
b) arachidonic amid tahat are precursor of prostaglandins and TX from 2 series
and LT from 4 series.
PGs (eg PGE2) that are synthesized from arachidonic acid, have both - anti
inflammatory and proinflammatory function. It is a possible to all this PG, always
are able to protect against noxius agents?
3. Page 5 and 6
What was a reason of application of two columns and conditions of fatty acids
separation from different tissues?
4. Page 8 and 9 - That are incomprehensible recordings.- Is C 14:19 cis - mean
C14 : 1cis-9? Or what is mean- C14:19,12c? or C 20 :01?
Level of interest: An article of limited interest
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
assistant