Reviewer's report
Title:HMGB1 cytoplasmic translocation in patients with acute liver failure
Version: 1Date:20 December 2010
Reviewer: Allan Tsung
Reviewer's report:
In this paper, Zhou and colleagues examine HMGB1 cytoplasmic translocation in
acute liver failure. Several issues that should be addressed:
Minor Essential Revisions
1. Page 6: The authors mention the use of murine macrophages (RAW 264.7
cells), however, there are no experiments using RAW cells in the results.
2. Page 11: The listing of figures in figure 1 should be in alphabetical order- from
A to F, not B to A, D and F to C and E.
3. Page 11-12: LPS induced HMGB1 release at a peak concentration of
400ng/ml. Why are different doses of LPS used for other experiments? (200ng/ml
for figure 1B and 100ng/ml for figure 2 and figure 3.)
4. Page 11-12: Why were HepG2 cells used instead of hepatocytes? It is much
more relavent to the in vivo experiments.
5. Page 12: Figure 5A is not mentioned?
6. Multiple grammatical errors need to be corrected.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
'I declare that I have no competing interests'
Reviewer's report
Title:HMGB1 cytoplasmic translocation in patients with acute liver failure
Version: 1Date:20 December 2010
Reviewer: Allan Tsung
Reviewer's report:
In this paper, Zhou and colleagues examine HMGB1 cytoplasmic translocation in
acute liver failure. Several issues that should be addressed:
Minor Essential Revisions
1. Page 6: The authors mention the use of murine macrophages (RAW 264.7
cells), however, there are no experiments using RAW cells in the results.
2. Page 11: The listing of figures in figure 1 should be in alphabetical order- from
A to F, not B to A, D and F to C and E.
3. Page 11-12: LPS induced HMGB1 release at a peak concentration of
400ng/ml. Why are different doses of LPS used for other experiments? (200ng/ml
for figure 1B and 100ng/ml for figure 2 and figure 3.)
4. Page 11-12: Why were HepG2 cells used instead of hepatocytes? It is much
more relavent to the in vivo experiments.
5. Page 12: Figure 5A is not mentioned?
6. Multiple grammatical errors need to be corrected.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
'I declare that I have no competing interests'
Please review this paper.
user
Reviewer's report
Title:HMGB1 cytoplasmic translocation in patients with acute liver failure
Version: 1Date:20 December 2010
Reviewer: Allan Tsung
Reviewer's report:
In this paper, Zhou and colleagues examine HMGB1 cytoplasmic translocation in
acute liver failure. Several issues that should be addressed:
Minor Essential Revisions
1. Page 6: The authors mention the use of murine macrophages (RAW 264.7
cells), however, there are no experiments using RAW cells in the results.
2. Page 11: The listing of figures in figure 1 should be in alphabetical order- from
A to F, not B to A, D and F to C and E.
3. Page 11-12: LPS induced HMGB1 release at a peak concentration of
400ng/ml. Why are different doses of LPS used for other experiments? (200ng/ml
for figure 1B and 100ng/ml for figure 2 and figure 3.)
4. Page 11-12: Why were HepG2 cells used instead of hepatocytes? It is much
more relavent to the in vivo experiments.
5. Page 12: Figure 5A is not mentioned?
6. Multiple grammatical errors need to be corrected.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
'I declare that I have no competing interests'
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