Title: Nuclear morphometry and chromatin texture changes in hepatocellular carcinoma samples
may predict liver transplant patients’ outcomes
Version: 2 Date: 14 Feb 2022
Reviewer's report:
Title: Nuclear morphometry and chromatin texture changes in hepatocellular carcinoma samples
may predict liver transplant patients’ outcomes
Authors: Jordan Boeira dos Santos et al.
Reviewer’s comments:
General remarks:
In this manuscript, the authors tried to analyze the nuclear characteristics of hepatocellular
carcinoma in relation to the recurrence rate after liver transplantation. This manuscript is clearly
described and the contents are significant. However, several parts should be dissolved as a
revised manuscript.
Major points:
1. Feret diameter and aspect ratio (AR) or nuclear perimeter are close indicators of nuclear
irregularity. However, either the perimeter or AR did not correlate with patients’ survival after
liver implantation. The authors should discuss the reason why either the perimeter or AR did
not correlate with patients’ survival even though the Feret diameter showed a correlation.
2. I agree that several nuclear factors showed differences in several characteristics (Sex, Age,
MELD score, Recurrence, and survival). However, none of them (nuclear factors) could be
the common factors for each characteristic. If so, why don’t you use discriminant analysis by
combining several factors to predict the difference for each characteristic?
3. I wonder if each HE stained specimen showed different nuclear intensity. Thus nuclear
detection setting for each specimen would be different. However, the authors did not show any
image analysis setting condition for morphometric analysis. This is pretty strange for us. Please
indicate the settings and representative image acquisition findings as new Table and Figure.
Minor points:
1. Please indicate the formula of MELD score in the Materials and Methods part.
2. Please indicate the formula of Aspect ratio (AR) in the Materials and Methods part.
3. Please indicate the definition and meanings of Feret diameter even if the authors take it for
granted in the Materials and Methods part.
4. In Line 176, “carried” is miss-typed as “caried”.
Title: Nuclear morphometry and chromatin texture changes in hepatocellular carcinoma samples
may predict liver transplant patients’ outcomes
Version: 2 Date: 14 Feb 2022
Reviewer's report:
Title: Nuclear morphometry and chromatin texture changes in hepatocellular carcinoma samples
may predict liver transplant patients’ outcomes
Authors: Jordan Boeira dos Santos et al.
Reviewer’s comments:
General remarks:
In this manuscript, the authors tried to analyze the nuclear characteristics of hepatocellular
carcinoma in relation to the recurrence rate after liver transplantation. This manuscript is clearly
described and the contents are significant. However, several parts should be dissolved as a
revised manuscript.
Major points:
1. Feret diameter and aspect ratio (AR) or nuclear perimeter are close indicators of nuclear
irregularity. However, either the perimeter or AR did not correlate with patients’ survival after
liver implantation. The authors should discuss the reason why either the perimeter or AR did
not correlate with patients’ survival even though the Feret diameter showed a correlation.
2. I agree that several nuclear factors showed differences in several characteristics (Sex, Age,
MELD score, Recurrence, and survival). However, none of them (nuclear factors) could be
the common factors for each characteristic. If so, why don’t you use discriminant analysis by
combining several factors to predict the difference for each characteristic?
3. I wonder if each HE stained specimen showed different nuclear intensity. Thus nuclear
detection setting for each specimen would be different. However, the authors did not show any
image analysis setting condition for morphometric analysis. This is pretty strange for us. Please
indicate the settings and representative image acquisition findings as new Table and Figure.
Minor points:
1. Please indicate the formula of MELD score in the Materials and Methods part.
2. Please indicate the formula of Aspect ratio (AR) in the Materials and Methods part.
3. Please indicate the definition and meanings of Feret diameter even if the authors take it for
granted in the Materials and Methods part.
4. In Line 176, “carried” is miss-typed as “caried”.
Please review this paper.
user
Title: Nuclear morphometry and chromatin texture changes in hepatocellular carcinoma samples
may predict liver transplant patients’ outcomes
Version: 2 Date: 14 Feb 2022
Reviewer's report:
Title: Nuclear morphometry and chromatin texture changes in hepatocellular carcinoma samples
may predict liver transplant patients’ outcomes
Authors: Jordan Boeira dos Santos et al.
Reviewer’s comments:
General remarks:
In this manuscript, the authors tried to analyze the nuclear characteristics of hepatocellular
carcinoma in relation to the recurrence rate after liver transplantation. This manuscript is clearly
described and the contents are significant. However, several parts should be dissolved as a
revised manuscript.
Major points:
1. Feret diameter and aspect ratio (AR) or nuclear perimeter are close indicators of nuclear
irregularity. However, either the perimeter or AR did not correlate with patients’ survival after
liver implantation. The authors should discuss the reason why either the perimeter or AR did
not correlate with patients’ survival even though the Feret diameter showed a correlation.
2. I agree that several nuclear factors showed differences in several characteristics (Sex, Age,
MELD score, Recurrence, and survival). However, none of them (nuclear factors) could be
the common factors for each characteristic. If so, why don’t you use discriminant analysis by
combining several factors to predict the difference for each characteristic?
3. I wonder if each HE stained specimen showed different nuclear intensity. Thus nuclear
detection setting for each specimen would be different. However, the authors did not show any
image analysis setting condition for morphometric analysis. This is pretty strange for us. Please
indicate the settings and representative image acquisition findings as new Table and Figure.
Minor points:
1. Please indicate the formula of MELD score in the Materials and Methods part.
2. Please indicate the formula of Aspect ratio (AR) in the Materials and Methods part.
3. Please indicate the definition and meanings of Feret diameter even if the authors take it for
granted in the Materials and Methods part.
4. In Line 176, “carried” is miss-typed as “caried”.
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