Title: Plasma and fecal zonulin are not altered by a high green leafy vegetable dietary
intervention: secondary analysis of a randomized control crossover trial.
Version: 4 Date: 20 Jan 2022
Reviewer's report:
The manuscript by Riviere et al. on the effect o a green leafy diet on plasma and fecal zonulin,
inflammatory biomarkers, and the fecal microbiome has been substantially revised and has
dramatically improved.
There are some minor points to be addressed for the article to be improved:
1. Abstract: please omit the abbreviations INT and CON and use the full terms (intervention
or control, respectively). The results section of the abstract needs to be substantially revised
to include baseline correlations of zonulin and inflammation and present the actual biomarkers
changes.
2. Statistical analysis: parametric tests are not appropriate for use in such a small sample size.
Please report only non-parametric tests results. Also, multiple a
3. Table 1: please add more baseline information on study participants - laboratory and
background medical conditions.
4. Please discuss in-depth the clinical significance of zonulin concerning systemic inflammation
and its interaction with the gut microbiome.
5. Why are the analyses between zonulin and inflammatory biomarkers reported across sex
strata? Please report associations first in the entire cohort. If there was an interaction on the
observed associations across sex strata, please state so.
6. Please report CRP in ng/L since this is more acceptable.
7. Title: please revise to highlight that this manuscript deals with plasma and fecal zonulin
changes and their clinical relevance. As reflected from the manuscript, the associations with
systemic inflammation and the gut microbiome were secondary aims.
8. Please also report baseline associations between zonulin and different biomarkers to highlight
the clinical relevance of this biomarker.
9. The manuscript needs to undergo English language editing.
Title: Plasma and fecal zonulin are not altered by a high green leafy vegetable dietary
intervention: secondary analysis of a randomized control crossover trial.
Version: 4 Date: 20 Jan 2022
Reviewer's report:
The manuscript by Riviere et al. on the effect o a green leafy diet on plasma and fecal zonulin,
inflammatory biomarkers, and the fecal microbiome has been substantially revised and has
dramatically improved.
There are some minor points to be addressed for the article to be improved:
1. Abstract: please omit the abbreviations INT and CON and use the full terms (intervention
or control, respectively). The results section of the abstract needs to be substantially revised
to include baseline correlations of zonulin and inflammation and present the actual biomarkers
changes.
2. Statistical analysis: parametric tests are not appropriate for use in such a small sample size.
Please report only non-parametric tests results. Also, multiple a
3. Table 1: please add more baseline information on study participants - laboratory and
background medical conditions.
4. Please discuss in-depth the clinical significance of zonulin concerning systemic inflammation
and its interaction with the gut microbiome.
5. Why are the analyses between zonulin and inflammatory biomarkers reported across sex
strata? Please report associations first in the entire cohort. If there was an interaction on the
observed associations across sex strata, please state so.
6. Please report CRP in ng/L since this is more acceptable.
7. Title: please revise to highlight that this manuscript deals with plasma and fecal zonulin
changes and their clinical relevance. As reflected from the manuscript, the associations with
systemic inflammation and the gut microbiome were secondary aims.
8. Please also report baseline associations between zonulin and different biomarkers to highlight
the clinical relevance of this biomarker.
9. The manuscript needs to undergo English language editing.
Please review this paper.
user
Title: Plasma and fecal zonulin are not altered by a high green leafy vegetable dietary
intervention: secondary analysis of a randomized control crossover trial.
Version: 4 Date: 20 Jan 2022
Reviewer's report:
The manuscript by Riviere et al. on the effect o a green leafy diet on plasma and fecal zonulin,
inflammatory biomarkers, and the fecal microbiome has been substantially revised and has
dramatically improved.
There are some minor points to be addressed for the article to be improved:
1. Abstract: please omit the abbreviations INT and CON and use the full terms (intervention
or control, respectively). The results section of the abstract needs to be substantially revised
to include baseline correlations of zonulin and inflammation and present the actual biomarkers
changes.
2. Statistical analysis: parametric tests are not appropriate for use in such a small sample size.
Please report only non-parametric tests results. Also, multiple a
3. Table 1: please add more baseline information on study participants - laboratory and
background medical conditions.
4. Please discuss in-depth the clinical significance of zonulin concerning systemic inflammation
and its interaction with the gut microbiome.
5. Why are the analyses between zonulin and inflammatory biomarkers reported across sex
strata? Please report associations first in the entire cohort. If there was an interaction on the
observed associations across sex strata, please state so.
6. Please report CRP in ng/L since this is more acceptable.
7. Title: please revise to highlight that this manuscript deals with plasma and fecal zonulin
changes and their clinical relevance. As reflected from the manuscript, the associations with
systemic inflammation and the gut microbiome were secondary aims.
8. Please also report baseline associations between zonulin and different biomarkers to highlight
the clinical relevance of this biomarker.
9. The manuscript needs to undergo English language editing.
assistant