Reviewer's report
Title:Induction of G1 and G2/M cell cycle arrests by the dietary compound
3,3'-diindolylmethane in HT-29 human colon cancer cells
Version: 1Date:11 January 2009
Reviewer: Thomas Szekeres
Reviewer's report:
Induction of G1 and G2/M cell cycle arrests by the dietary compound 3, 3’
diindolylmethane in HT-29 human colon cancer cells by Choi et al.
The authors describe the effects of 3, 3’ diindolylmethane on the cell cycle
progression in human colon cancer cells (HT-29). The data and results presented
are interesting, however I would suggest extending this study and including
additional results, as the results presented are describing the effects of one
compound in one cell line at one concentration. The concentration of 30 uM used
for the experiments is 10 times higher than the concentration which can be
achieved and therefore additional data are necessary to draw the conclusions
stated by the authors. I would suggest including results with lower concentrations
prior to publication of this manuscript.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Reviewer's report
Title:Induction of G1 and G2/M cell cycle arrests by the dietary compound
3,3'-diindolylmethane in HT-29 human colon cancer cells
Version: 1Date:11 January 2009
Reviewer: Thomas Szekeres
Reviewer's report:
Induction of G1 and G2/M cell cycle arrests by the dietary compound 3, 3’
diindolylmethane in HT-29 human colon cancer cells by Choi et al.
The authors describe the effects of 3, 3’ diindolylmethane on the cell cycle
progression in human colon cancer cells (HT-29). The data and results presented
are interesting, however I would suggest extending this study and including
additional results, as the results presented are describing the effects of one
compound in one cell line at one concentration. The concentration of 30 uM used
for the experiments is 10 times higher than the concentration which can be
achieved and therefore additional data are necessary to draw the conclusions
stated by the authors. I would suggest including results with lower concentrations
prior to publication of this manuscript.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Please review this paper.
user
Reviewer's report
Title:Induction of G1 and G2/M cell cycle arrests by the dietary compound
3,3'-diindolylmethane in HT-29 human colon cancer cells
Version: 1Date:11 January 2009
Reviewer: Thomas Szekeres
Reviewer's report:
Induction of G1 and G2/M cell cycle arrests by the dietary compound 3, 3’
diindolylmethane in HT-29 human colon cancer cells by Choi et al.
The authors describe the effects of 3, 3’ diindolylmethane on the cell cycle
progression in human colon cancer cells (HT-29). The data and results presented
are interesting, however I would suggest extending this study and including
additional results, as the results presented are describing the effects of one
compound in one cell line at one concentration. The concentration of 30 uM used
for the experiments is 10 times higher than the concentration which can be
achieved and therefore additional data are necessary to draw the conclusions
stated by the authors. I would suggest including results with lower concentrations
prior to publication of this manuscript.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
assistant