Reviewer's report
Title:Paraoxonase-1 is related to inflammation, fibrosis and PPAR delta in
experimental liver disease
Version: 2Date:2 December 2008
Reviewer: Fabio Marra
Reviewer's report:
The revised version of this manuscript is markedly improved by the addition of
sentences that better explain the findings and allow to put forward some
hypotheses for their interpretation.
Yet, this Reviewer feels that some additional efforts could be made to support the
hypotheses and speculation made in the Discussion. Specifically, and in relation
to point#1 of the previous review:
1. I wonder whether the Authors could run a simple Western blot analysis with
PON1 antibodies, as they have already done in other studies (e.g. J Gastrointest
Surg 2008;12:891). Apparently, this would also allow the Authors to establish
whether PON1 proteolysis is indeed reduced, as hypothesized.
2. Another piece of information could derive from analysis of HDL levels, that are
said to be decreased, but no experimental indication of this fact is provided.
At least one of these approaches would make the study stronger.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Paraoxonase-1 is related to inflammation, fibrosis and PPAR delta in
experimental liver disease
Version: 2Date:2 December 2008
Reviewer: Fabio Marra
Reviewer's report:
The revised version of this manuscript is markedly improved by the addition of
sentences that better explain the findings and allow to put forward some
hypotheses for their interpretation.
Yet, this Reviewer feels that some additional efforts could be made to support the
hypotheses and speculation made in the Discussion. Specifically, and in relation
to point#1 of the previous review:
1. I wonder whether the Authors could run a simple Western blot analysis with
PON1 antibodies, as they have already done in other studies (e.g. J Gastrointest
Surg 2008;12:891). Apparently, this would also allow the Authors to establish
whether PON1 proteolysis is indeed reduced, as hypothesized.
2. Another piece of information could derive from analysis of HDL levels, that are
said to be decreased, but no experimental indication of this fact is provided.
At least one of these approaches would make the study stronger.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Paraoxonase-1 is related to inflammation, fibrosis and PPAR delta in
experimental liver disease
Version: 2Date:2 December 2008
Reviewer: Fabio Marra
Reviewer's report:
The revised version of this manuscript is markedly improved by the addition of
sentences that better explain the findings and allow to put forward some
hypotheses for their interpretation.
Yet, this Reviewer feels that some additional efforts could be made to support the
hypotheses and speculation made in the Discussion. Specifically, and in relation
to point#1 of the previous review:
1. I wonder whether the Authors could run a simple Western blot analysis with
PON1 antibodies, as they have already done in other studies (e.g. J Gastrointest
Surg 2008;12:891). Apparently, this would also allow the Authors to establish
whether PON1 proteolysis is indeed reduced, as hypothesized.
2. Another piece of information could derive from analysis of HDL levels, that are
said to be decreased, but no experimental indication of this fact is provided.
At least one of these approaches would make the study stronger.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
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