Title: Title# Defibrotide improved outcome of monocrotaline induced rat hepatic sinusoidal
obstruction syndrome
Version: 2 Date: 05 Jul 2022
Reviewer's report:
The authors attempt to test therapeutic effect of Defibrotide on amending liver injury with
hepatic sinusoidal obstruction syndrome in rat. Well, Sinusoidal obstruction syndrome is a veno-
occlusive disease arises in liver. Therapeutic strategies to ameliorate HSOS remain to develop. In
this study, monocrotaline (MCT) administration to rat was applied to establish hepatic sinusoidal
obstruction syndrome (HSOS) diseased rat model to test the therapeutic effect of Defibrotide
on treating HSOS.
Description is insufficient and unclear in the text. Linkage of data to explanation is inadequate.
For examples, The Fig 1. is not adapted to text and subtitle 3.1 narrated gross anatomy. Anatomic
pictures did not display in this manuscript. Also English writing is not good enough. English
proofreading is required.
Line 38-39 Defibrotide was efficient for HSOS followed hematopoietic stem cell
transplantation.
HSOS being caused by hematopoietic stem cell transplantation did not have the equivalent cell
damages in liver with HSOS by MCT administration.
Line 42-47
The method paragraph abstract
Should drop more ward to describe what methods the authors applied to biochemical analysis.
In second paragraph in abstract, the authors did only write the animal grouping
Line 185
The subtitle 3.1 “The gross anatomy and pathology characteristics of MCT induced HSOS”
The authors showed only histochemical staining but none of data for gross anatomy
Line 187-193 none of data are available for those words.
Line 282
“We created an MCT induced HSOS rat model………….”
The way that MCT induced HSOS rat was reported previous studies. The authors are not the
first one who established this animal model.
Line 301 “Defibrotide has been proved to stabilize and protect endothelial cells against
activation”
The mean of this sentence is not clear. What is therapeutic effect of Defibrotide on stabilizing
and protecting endothelial cells against activation. What kind of activation that endothelial cells
do against?
Title: Title# Defibrotide improved outcome of monocrotaline induced rat hepatic sinusoidal
obstruction syndrome
Version: 2 Date: 05 Jul 2022
Reviewer's report:
The authors attempt to test therapeutic effect of Defibrotide on amending liver injury with
hepatic sinusoidal obstruction syndrome in rat. Well, Sinusoidal obstruction syndrome is a veno-
occlusive disease arises in liver. Therapeutic strategies to ameliorate HSOS remain to develop. In
this study, monocrotaline (MCT) administration to rat was applied to establish hepatic sinusoidal
obstruction syndrome (HSOS) diseased rat model to test the therapeutic effect of Defibrotide
on treating HSOS.
Description is insufficient and unclear in the text. Linkage of data to explanation is inadequate.
For examples, The Fig 1. is not adapted to text and subtitle 3.1 narrated gross anatomy. Anatomic
pictures did not display in this manuscript. Also English writing is not good enough. English
proofreading is required.
Line 38-39 Defibrotide was efficient for HSOS followed hematopoietic stem cell
transplantation.
HSOS being caused by hematopoietic stem cell transplantation did not have the equivalent cell
damages in liver with HSOS by MCT administration.
Line 42-47
The method paragraph abstract
Should drop more ward to describe what methods the authors applied to biochemical analysis.
In second paragraph in abstract, the authors did only write the animal grouping
Line 185
The subtitle 3.1 “The gross anatomy and pathology characteristics of MCT induced HSOS”
The authors showed only histochemical staining but none of data for gross anatomy
Line 187-193 none of data are available for those words.
Line 282
“We created an MCT induced HSOS rat model………….”
The way that MCT induced HSOS rat was reported previous studies. The authors are not the
first one who established this animal model.
Line 301 “Defibrotide has been proved to stabilize and protect endothelial cells against
activation”
The mean of this sentence is not clear. What is therapeutic effect of Defibrotide on stabilizing
and protecting endothelial cells against activation. What kind of activation that endothelial cells
do against?
Please review this paper.
user
Title: Title# Defibrotide improved outcome of monocrotaline induced rat hepatic sinusoidal
obstruction syndrome
Version: 2 Date: 05 Jul 2022
Reviewer's report:
The authors attempt to test therapeutic effect of Defibrotide on amending liver injury with
hepatic sinusoidal obstruction syndrome in rat. Well, Sinusoidal obstruction syndrome is a veno-
occlusive disease arises in liver. Therapeutic strategies to ameliorate HSOS remain to develop. In
this study, monocrotaline (MCT) administration to rat was applied to establish hepatic sinusoidal
obstruction syndrome (HSOS) diseased rat model to test the therapeutic effect of Defibrotide
on treating HSOS.
Description is insufficient and unclear in the text. Linkage of data to explanation is inadequate.
For examples, The Fig 1. is not adapted to text and subtitle 3.1 narrated gross anatomy. Anatomic
pictures did not display in this manuscript. Also English writing is not good enough. English
proofreading is required.
Line 38-39 Defibrotide was efficient for HSOS followed hematopoietic stem cell
transplantation.
HSOS being caused by hematopoietic stem cell transplantation did not have the equivalent cell
damages in liver with HSOS by MCT administration.
Line 42-47
The method paragraph abstract
Should drop more ward to describe what methods the authors applied to biochemical analysis.
In second paragraph in abstract, the authors did only write the animal grouping
Line 185
The subtitle 3.1 “The gross anatomy and pathology characteristics of MCT induced HSOS”
The authors showed only histochemical staining but none of data for gross anatomy
Line 187-193 none of data are available for those words.
Line 282
“We created an MCT induced HSOS rat model………….”
The way that MCT induced HSOS rat was reported previous studies. The authors are not the
first one who established this animal model.
Line 301 “Defibrotide has been proved to stabilize and protect endothelial cells against
activation”
The mean of this sentence is not clear. What is therapeutic effect of Defibrotide on stabilizing
and protecting endothelial cells against activation. What kind of activation that endothelial cells
do against?
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