Reviewer's report
Title:DPO multiplex PCR as an alternative to culture and susceptibility testing to
detect Helicobacter pylori and its resistance to clarithromycin.
Version: 1Date:22 August 2011
Reviewer: Michael Selgrad
Reviewer's report:
The authors describe that the Seeplex method can be used in laboratories that
are not able to perform real time pcr.
I wonder about the costs of the new method. Is it really an afforadble tool?
Furthermore I would like to raise one point. Clarihtromycin resistance is of course
the most important factor for failure of H. pylori treatment. However, antibiotic
resistance of the other used antibiotics is also rising. According to international
recommendations, H. pylori suscepitibilty testing should performed after the
failure of second line treatment. Thus, it is mandatory to get to know the
susceptibilty of the other antibiotics, which might be used as salvage therapy.
This test method only detects clarithromycin resistance which in my opinion
lowers the practical application of the test method.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Reviewer's report
Title:DPO multiplex PCR as an alternative to culture and susceptibility testing to
detect Helicobacter pylori and its resistance to clarithromycin.
Version: 1Date:22 August 2011
Reviewer: Michael Selgrad
Reviewer's report:
The authors describe that the Seeplex method can be used in laboratories that
are not able to perform real time pcr.
I wonder about the costs of the new method. Is it really an afforadble tool?
Furthermore I would like to raise one point. Clarihtromycin resistance is of course
the most important factor for failure of H. pylori treatment. However, antibiotic
resistance of the other used antibiotics is also rising. According to international
recommendations, H. pylori suscepitibilty testing should performed after the
failure of second line treatment. Thus, it is mandatory to get to know the
susceptibilty of the other antibiotics, which might be used as salvage therapy.
This test method only detects clarithromycin resistance which in my opinion
lowers the practical application of the test method.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Please review this paper.
user
Reviewer's report
Title:DPO multiplex PCR as an alternative to culture and susceptibility testing to
detect Helicobacter pylori and its resistance to clarithromycin.
Version: 1Date:22 August 2011
Reviewer: Michael Selgrad
Reviewer's report:
The authors describe that the Seeplex method can be used in laboratories that
are not able to perform real time pcr.
I wonder about the costs of the new method. Is it really an afforadble tool?
Furthermore I would like to raise one point. Clarihtromycin resistance is of course
the most important factor for failure of H. pylori treatment. However, antibiotic
resistance of the other used antibiotics is also rising. According to international
recommendations, H. pylori suscepitibilty testing should performed after the
failure of second line treatment. Thus, it is mandatory to get to know the
susceptibilty of the other antibiotics, which might be used as salvage therapy.
This test method only detects clarithromycin resistance which in my opinion
lowers the practical application of the test method.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
assistant