Reviewer's report
Title:Transporter function and cyclic AMP turnover in normal colonic mucosa
from patients with and without colorectal neoplasia
Version: 2Date:1 May 2012
Reviewer: Kacper Wojtal
Reviewer's report:
Minor Essential Revisions:
The fact that db-cAMP had no effect on SCC when applied to the apical side
does not directly disprove the fact that it is cell-permeable analog, which is in my
opinion still the case. The lack of effect may be simply due to specific mucosal
barrier properties, which create limited diffusion barrer for these compounds as
compared to basolateral side. Nevertheless, I understand the rationale of the
authors when trying to correlate the effect of cAMPs and the presence of cNTs at
the basal surface, which is in my opinon difficult to disprove afterall.
Hence, I suggest to modify the existing statement (Page 8, Section: 'Functional
characterization of transport'; line 9) into the following:
...indicating db-cAMP as a non-permeable analog in our experimental setting and
implying the transport mechanism at the balolateral membrane.
Level of interest: An article of outstanding merit and interest in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Transporter function and cyclic AMP turnover in normal colonic mucosa
from patients with and without colorectal neoplasia
Version: 2Date:1 May 2012
Reviewer: Kacper Wojtal
Reviewer's report:
Minor Essential Revisions:
The fact that db-cAMP had no effect on SCC when applied to the apical side
does not directly disprove the fact that it is cell-permeable analog, which is in my
opinion still the case. The lack of effect may be simply due to specific mucosal
barrier properties, which create limited diffusion barrer for these compounds as
compared to basolateral side. Nevertheless, I understand the rationale of the
authors when trying to correlate the effect of cAMPs and the presence of cNTs at
the basal surface, which is in my opinon difficult to disprove afterall.
Hence, I suggest to modify the existing statement (Page 8, Section: 'Functional
characterization of transport'; line 9) into the following:
...indicating db-cAMP as a non-permeable analog in our experimental setting and
implying the transport mechanism at the balolateral membrane.
Level of interest: An article of outstanding merit and interest in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Transporter function and cyclic AMP turnover in normal colonic mucosa
from patients with and without colorectal neoplasia
Version: 2Date:1 May 2012
Reviewer: Kacper Wojtal
Reviewer's report:
Minor Essential Revisions:
The fact that db-cAMP had no effect on SCC when applied to the apical side
does not directly disprove the fact that it is cell-permeable analog, which is in my
opinion still the case. The lack of effect may be simply due to specific mucosal
barrier properties, which create limited diffusion barrer for these compounds as
compared to basolateral side. Nevertheless, I understand the rationale of the
authors when trying to correlate the effect of cAMPs and the presence of cNTs at
the basal surface, which is in my opinon difficult to disprove afterall.
Hence, I suggest to modify the existing statement (Page 8, Section: 'Functional
characterization of transport'; line 9) into the following:
...indicating db-cAMP as a non-permeable analog in our experimental setting and
implying the transport mechanism at the balolateral membrane.
Level of interest: An article of outstanding merit and interest in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
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