Title: Deciphering the Integrated Immunogenomic Landscape of Colorectal Cancer: Insights
from Mendelian Randomization and Immune-Stratified Molecular Subtyping
Version: 3 Date: 03 Sep 2024
Reviewer's report:
1. The authors did not use FDR or other correction methods, please emphasize the limitation.
2. External Validation set is lacked in the prognostic signature.
3. Colony formation assays: lacked replications and statistical significance.
4. siRNA-lacked Western blot validation.
5. Annexin V-PI result: gate in the upper right corner is currently regarded as expressing
apoptosis with questionable accuracy, and it is suggested that the result be deleted if there is no
discrepancy in the lower right corner.
Title: Deciphering the Integrated Immunogenomic Landscape of Colorectal Cancer: Insights
from Mendelian Randomization and Immune-Stratified Molecular Subtyping
Version: 3 Date: 03 Sep 2024
Reviewer's report:
1. The authors did not use FDR or other correction methods, please emphasize the limitation.
2. External Validation set is lacked in the prognostic signature.
3. Colony formation assays: lacked replications and statistical significance.
4. siRNA-lacked Western blot validation.
5. Annexin V-PI result: gate in the upper right corner is currently regarded as expressing
apoptosis with questionable accuracy, and it is suggested that the result be deleted if there is no
discrepancy in the lower right corner.
Please review this paper.
user
Title: Deciphering the Integrated Immunogenomic Landscape of Colorectal Cancer: Insights
from Mendelian Randomization and Immune-Stratified Molecular Subtyping
Version: 3 Date: 03 Sep 2024
Reviewer's report:
1. The authors did not use FDR or other correction methods, please emphasize the limitation.
2. External Validation set is lacked in the prognostic signature.
3. Colony formation assays: lacked replications and statistical significance.
4. siRNA-lacked Western blot validation.
5. Annexin V-PI result: gate in the upper right corner is currently regarded as expressing
apoptosis with questionable accuracy, and it is suggested that the result be deleted if there is no
discrepancy in the lower right corner.
assistant