Reviewer's report
Title:Supplementation with vitamin E (alpha-tocopherol) in diabetic rats: effects
on the proximal colon
Version: 2Date:14 August 2009
Reviewer: MC C SABU
Reviewer's report:
Major Compulsory Revisions
1. The author has not corrected the term Balanced, Padronized and
Standardized chow to common term “Padronized chow” in the text as they
replyed
2. What is the initial body weight of animals used? What is the rational of Vitamin
E 900 mg/animals administration for long term animal experiments? What is the
LD50 of Vitamin E. Give any reference for the high dose of Vitamin E treatment.
3. There is no use of showing only the normal and diabetic group
photomicrographies (Fig 3). It is important to include the photomicrographies for
other treatment groups also. Or else the author omits the fig 3, from the text.
4. If the experiment of the typical clinical manifestations has been already carried
out in the author’s laboratory and confirmed with their earlier data, then why the
author repeated the measurement in this study. It is not necessary to mention in
the discussion section (1st para) too. It is important to include the data for long
term study.
5. From the author’s report the low dose of Vitamin E treatment is not effective
(glycemia and glycated haemoglobin level) when compared with diabetic group
(Table 1). The values are not just a numeric difference (as author said) and
eventhough it is not significant both the values are higher than diabetic control
group. The data is not agreeable.Or it mean the 0.1% of Vit E is not having action
on diabetic rats.
Reviewer's report
Title:Supplementation with vitamin E (alpha-tocopherol) in diabetic rats: effects
on the proximal colon
Version: 2Date:14 August 2009
Reviewer: MC C SABU
Reviewer's report:
Major Compulsory Revisions
1. The author has not corrected the term Balanced, Padronized and
Standardized chow to common term “Padronized chow” in the text as they
replyed
2. What is the initial body weight of animals used? What is the rational of Vitamin
E 900 mg/animals administration for long term animal experiments? What is the
LD50 of Vitamin E. Give any reference for the high dose of Vitamin E treatment.
3. There is no use of showing only the normal and diabetic group
photomicrographies (Fig 3). It is important to include the photomicrographies for
other treatment groups also. Or else the author omits the fig 3, from the text.
4. If the experiment of the typical clinical manifestations has been already carried
out in the author’s laboratory and confirmed with their earlier data, then why the
author repeated the measurement in this study. It is not necessary to mention in
the discussion section (1st para) too. It is important to include the data for long
term study.
5. From the author’s report the low dose of Vitamin E treatment is not effective
(glycemia and glycated haemoglobin level) when compared with diabetic group
(Table 1). The values are not just a numeric difference (as author said) and
eventhough it is not significant both the values are higher than diabetic control
group. The data is not agreeable.Or it mean the 0.1% of Vit E is not having action
on diabetic rats.
Please review this paper.
user
Reviewer's report
Title:Supplementation with vitamin E (alpha-tocopherol) in diabetic rats: effects
on the proximal colon
Version: 2Date:14 August 2009
Reviewer: MC C SABU
Reviewer's report:
Major Compulsory Revisions
1. The author has not corrected the term Balanced, Padronized and
Standardized chow to common term “Padronized chow” in the text as they
replyed
2. What is the initial body weight of animals used? What is the rational of Vitamin
E 900 mg/animals administration for long term animal experiments? What is the
LD50 of Vitamin E. Give any reference for the high dose of Vitamin E treatment.
3. There is no use of showing only the normal and diabetic group
photomicrographies (Fig 3). It is important to include the photomicrographies for
other treatment groups also. Or else the author omits the fig 3, from the text.
4. If the experiment of the typical clinical manifestations has been already carried
out in the author’s laboratory and confirmed with their earlier data, then why the
author repeated the measurement in this study. It is not necessary to mention in
the discussion section (1st para) too. It is important to include the data for long
term study.
5. From the author’s report the low dose of Vitamin E treatment is not effective
(glycemia and glycated haemoglobin level) when compared with diabetic group
(Table 1). The values are not just a numeric difference (as author said) and
eventhough it is not significant both the values are higher than diabetic control
group. The data is not agreeable.Or it mean the 0.1% of Vit E is not having action
on diabetic rats.
assistant