Title: Identification and validation of a CD4 + T cell-related prognostic model to predict immune
responses in stage III-IV colorectal cancer
Version: 3 Date: 04 Jan 2025
Reviewer:  Wenlong Chen
Reviewer's report:
The authors used SingleR to annotate cell subpopulations, however manual annotation is
preferred. Marker gene expression in each cluster should be represented. The authors categorized
the cells in Figure2B,E as CD4+ T cells, T cells? What is the basis? because the latter includes
the former. The authors' analysis of single-cell data is puzzling.
Title: Identification and validation of a CD4 + T cell-related prognostic model to predict immune
responses in stage III-IV colorectal cancer
Version: 3 Date: 04 Jan 2025
Reviewer:  Wenlong Chen
Reviewer's report:
The authors used SingleR to annotate cell subpopulations, however manual annotation is
preferred. Marker gene expression in each cluster should be represented. The authors categorized
the cells in Figure2B,E as CD4+ T cells, T cells? What is the basis? because the latter includes
the former. The authors' analysis of single-cell data is puzzling.
Please review this paper.
user
Title: Identification and validation of a CD4 + T cell-related prognostic model to predict immune
responses in stage III-IV colorectal cancer
Version: 3 Date: 04 Jan 2025
Reviewer:  Wenlong Chen
Reviewer's report:
The authors used SingleR to annotate cell subpopulations, however manual annotation is
preferred. Marker gene expression in each cluster should be represented. The authors categorized
the cells in Figure2B,E as CD4+ T cells, T cells? What is the basis? because the latter includes
the former. The authors' analysis of single-cell data is puzzling.
assistant