Title: Comprehensive analysis of candidate signatures of long non-coding RNA LINC01116
and related protein-coding genes in patients with hepatocellular carcinoma
Version: 2 Date: 30 Jan 2023
Reviewer's report:
The authors examined the functions of long non-coding RNA LINC01116 and related protein-
coding genes in patients with hepatocellular carcinoma. This finding is particularly significant
for uncovering the role of this lncRNA in hepatocellular carcinoma pathology. The manuscript
is well technically, experimentally, and writing. However, there is a few comments:
1- In the abstract, “The diagnostic potentials were then validated by real time-PCR and
Oncomine cohorts.” Mentioned, but in the manuscript, there are no data and results of the real-
time PCR. Authors must mention it in “methods” section.
2- Did the authors check the normality (normal distribution) of the expression data?
3- Authors should mention the cut-off, sensitivity percentage, and specificity percentage (in
ROC curve analysis) in a table.
Title: Comprehensive analysis of candidate signatures of long non-coding RNA LINC01116
and related protein-coding genes in patients with hepatocellular carcinoma
Version: 2 Date: 30 Jan 2023
Reviewer's report:
The authors examined the functions of long non-coding RNA LINC01116 and related protein-
coding genes in patients with hepatocellular carcinoma. This finding is particularly significant
for uncovering the role of this lncRNA in hepatocellular carcinoma pathology. The manuscript
is well technically, experimentally, and writing. However, there is a few comments:
1- In the abstract, “The diagnostic potentials were then validated by real time-PCR and
Oncomine cohorts.” Mentioned, but in the manuscript, there are no data and results of the real-
time PCR. Authors must mention it in “methods” section.
2- Did the authors check the normality (normal distribution) of the expression data?
3- Authors should mention the cut-off, sensitivity percentage, and specificity percentage (in
ROC curve analysis) in a table.
Please review this paper.
user
Title: Comprehensive analysis of candidate signatures of long non-coding RNA LINC01116
and related protein-coding genes in patients with hepatocellular carcinoma
Version: 2 Date: 30 Jan 2023
Reviewer's report:
The authors examined the functions of long non-coding RNA LINC01116 and related protein-
coding genes in patients with hepatocellular carcinoma. This finding is particularly significant
for uncovering the role of this lncRNA in hepatocellular carcinoma pathology. The manuscript
is well technically, experimentally, and writing. However, there is a few comments:
1- In the abstract, “The diagnostic potentials were then validated by real time-PCR and
Oncomine cohorts.” Mentioned, but in the manuscript, there are no data and results of the real-
time PCR. Authors must mention it in “methods” section.
2- Did the authors check the normality (normal distribution) of the expression data?
3- Authors should mention the cut-off, sensitivity percentage, and specificity percentage (in
ROC curve analysis) in a table.
assistant