Reviewer's report
Title:Inhibitory effects of astaxanthin on azoxymethane-induced colonic
preneoplastic lesions in C57/BL/KsJ-db/db mice
Version:1Date:14 August 2014
Reviewer: Nagini Siddavaram
Reviewer's report:
Minor essential revisions.
Following is a detailed critique of the manuscript
Materials and methods
a) Page no 6 – line no 17: treatment with astaxanthin is mentioned as 8 weeks
but in abstract it is mentioned as 12 weeks. Please clarify
b) PCR conditions for real time RT-PCR should be mentioned
c) Page no 8 – line no 22: replace with 8-hydroxy-2#-deoxyguanosine
Results
Western blotting for SOD, GPx, IL-1###IL-6, and TNF-##should be included.
Discussion
Page no 14 – line no 6: replace with TNF-#
List of abbreviations
Line no 1: replace with 8-hydroxy-2#-deoxyguanosine
References
The reference list has to be updated. There are several papers on the subject
published in 2013 and 2014. Of relevance to this manuscript are recent papers
by Hong et al (2013), Tuominen et al (2013), Khan et al (2013), Kavitha et al
(2013) and Kochi et al (2014). The authors should discuss their data in the light
of these findings.
Figures
Figure 1: representative photomicrographs for ACF and BCAC for all groups
have to be included.
Format
Tables have to be presented after figures as per the journal format
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
Reviewer's report
Title:Inhibitory effects of astaxanthin on azoxymethane-induced colonic
preneoplastic lesions in C57/BL/KsJ-db/db mice
Version:1Date:14 August 2014
Reviewer: Nagini Siddavaram
Reviewer's report:
Minor essential revisions.
Following is a detailed critique of the manuscript
Materials and methods
a) Page no 6 – line no 17: treatment with astaxanthin is mentioned as 8 weeks
but in abstract it is mentioned as 12 weeks. Please clarify
b) PCR conditions for real time RT-PCR should be mentioned
c) Page no 8 – line no 22: replace with 8-hydroxy-2#-deoxyguanosine
Results
Western blotting for SOD, GPx, IL-1###IL-6, and TNF-##should be included.
Discussion
Page no 14 – line no 6: replace with TNF-#
List of abbreviations
Line no 1: replace with 8-hydroxy-2#-deoxyguanosine
References
The reference list has to be updated. There are several papers on the subject
published in 2013 and 2014. Of relevance to this manuscript are recent papers
by Hong et al (2013), Tuominen et al (2013), Khan et al (2013), Kavitha et al
(2013) and Kochi et al (2014). The authors should discuss their data in the light
of these findings.
Figures
Figure 1: representative photomicrographs for ACF and BCAC for all groups
have to be included.
Format
Tables have to be presented after figures as per the journal format
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
Please review this paper.
user
Reviewer's report
Title:Inhibitory effects of astaxanthin on azoxymethane-induced colonic
preneoplastic lesions in C57/BL/KsJ-db/db mice
Version:1Date:14 August 2014
Reviewer: Nagini Siddavaram
Reviewer's report:
Minor essential revisions.
Following is a detailed critique of the manuscript
Materials and methods
a) Page no 6 – line no 17: treatment with astaxanthin is mentioned as 8 weeks
but in abstract it is mentioned as 12 weeks. Please clarify
b) PCR conditions for real time RT-PCR should be mentioned
c) Page no 8 – line no 22: replace with 8-hydroxy-2#-deoxyguanosine
Results
Western blotting for SOD, GPx, IL-1###IL-6, and TNF-##should be included.
Discussion
Page no 14 – line no 6: replace with TNF-#
List of abbreviations
Line no 1: replace with 8-hydroxy-2#-deoxyguanosine
References
The reference list has to be updated. There are several papers on the subject
published in 2013 and 2014. Of relevance to this manuscript are recent papers
by Hong et al (2013), Tuominen et al (2013), Khan et al (2013), Kavitha et al
(2013) and Kochi et al (2014). The authors should discuss their data in the light
of these findings.
Figures
Figure 1: representative photomicrographs for ACF and BCAC for all groups
have to be included.
Format
Tables have to be presented after figures as per the journal format
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
assistant