Reviewer ’s report  
Title: The gut microbiome as a target for regulatory T cell -based immunotherapy: Induction of  
regulatory lymphocytes by oral administration of anti -LPS enriched colostrum alleviates immune  
mediated colitis  
 
Version: 0 Date: 19 Sep 2015  
Reviewer: Chen Shuan Chung  
Reviewer's report:  
It is an interesting study using oral  administration  of   Imm124E -induced colostrum to 
investigate the immune reponse in colitic mice model. However, there are some comments to this 
study:  
 
Major:  
1. The number of mice in each group is too small to draw a conclusion to the result since 
that some data did not sh ow significant difference between groups but only trends.  
 
2. In the method part, please describe the grading system for damage and regeneration 
scores in details.  
 
Minor:  
1. page 4 Grading of colon histology: There are some non -English letters at the endin g of 
the paragraph.  
 
2. page 3 line 50 Imm124Einduced -> Imm124E -induced  
 
3. page 4 line 25 mice were purchase -> mice were purchased  
 
4. page 5 line 15 4oC -> should be superscript character for "o".  
 
5. page 6 line 16,29,30 significantly and significant were  marked, please describe it why?  
 
6. page 9 line 7 Imm124Einduced -> imm124E induced  
Are the methods appropriate and well described?  
If not, please specify what is required in your comments to the authors.  
No 
Does the work include the necessary controls?  
If not, please specify which controls are re quired in your comments to the authors.  
Yes 
Are the conclusions drawn adequately supported by the data shown?  
If not, please explain in your comments to the authors.  
Unable to assess  
Are you able to assess any statistics in the manuscript or would you recommen d an 
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects require further  
assessment in your comments to the editors.  
I am able to assess the statistics  
Quality of written En glish  
Please indicate the quality of language in the manuscript:  
Acceptable  
Declaration of competing interests  
Please complete a declaration of competing interests, considering the following questions:  
1. Have you in the past five years received reimbursements, fees, funding, or salary from an 
organisation that may in any way gain or lose financially from the publication of this 
manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares in an organisation that may in any way gain or lose 
financially from the publication of this manuscript, either now or in th e future?  
 
3. Do you hold or are you currently applying for any patents relating to the content of the 
manuscript?  
 
4. Have you received reimbursements, fees, funding, or salary from an organization that 
holds or has applied for patents relating to the content of the manuscript?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have any non -financial competing interests in relation to this paper?  
If you can answer no to all of the above, write 'I declare that I have no competing interests' 
below. If your reply is yes to any, please give details below.  
I declare that I have no competing interests.  
I agree to the open peer review policy of the journal . I understand that my name will be included 
on my report to the authors and, if the manuscript is accepted for publication, my named report 
including any attachments I upload will be posted on the website along with the authors' 
responses. I agree for my report to be made available under an Open Acc ess Creative Commons 
CC-BY license ( http://creativecommons.org/licenses/by/4.0/ ). I understand that any comments 
which I do not wish to be included in my named report can be included as confidential comments 
to the editors, which will not be published.  
I agree to the open peer review policy of the journal  
Reviewer ’s report  
Title: The gut microbiome as a target for regulatory T cell -based immunotherapy: Induction of  
regulatory lymphocytes by oral administration of anti -LPS enriched colostrum alleviates immune  
mediated colitis  
 
Version: 0 Date: 19 Sep 2015  
Reviewer: Chen Shuan Chung  
Reviewer's report:  
It is an interesting study using oral  administration  of   Imm124E -induced colostrum to 
investigate the immune reponse in colitic mice model. However, there are some comments to this 
study:  
 
Major:  
1. The number of mice in each group is too small to draw a conclusion to the result since 
that some data did not sh ow significant difference between groups but only trends.  
 
2. In the method part, please describe the grading system for damage and regeneration 
scores in details.  
 
Minor:  
1. page 4 Grading of colon histology: There are some non -English letters at the endin g of 
the paragraph.  
 
2. page 3 line 50 Imm124Einduced -> Imm124E -induced  
 
3. page 4 line 25 mice were purchase -> mice were purchased  
 
4. page 5 line 15 4oC -> should be superscript character for "o".  
 
5. page 6 line 16,29,30 significantly and significant were  marked, please describe it why?  
 
6. page 9 line 7 Imm124Einduced -> imm124E induced  
Are the methods appropriate and well described?  
If not, please specify what is required in your comments to the authors.  
No 
Does the work include the necessary controls?  
If not, please specify which controls are re quired in your comments to the authors.  
Yes 
Are the conclusions drawn adequately supported by the data shown?  
If not, please explain in your comments to the authors.  
Unable to assess  
Are you able to assess any statistics in the manuscript or would you recommen d an 
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects require further  
assessment in your comments to the editors.  
I am able to assess the statistics  
Quality of written En glish  
Please indicate the quality of language in the manuscript:  
Acceptable  
Declaration of competing interests  
Please complete a declaration of competing interests, considering the following questions:  
1. Have you in the past five years received reimbursements, fees, funding, or salary from an 
organisation that may in any way gain or lose financially from the publication of this 
manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares in an organisation that may in any way gain or lose 
financially from the publication of this manuscript, either now or in th e future?  
 
3. Do you hold or are you currently applying for any patents relating to the content of the 
manuscript?  
 
4. Have you received reimbursements, fees, funding, or salary from an organization that 
holds or has applied for patents relating to the content of the manuscript?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have any non -financial competing interests in relation to this paper?  
If you can answer no to all of the above, write 'I declare that I have no competing interests' 
below. If your reply is yes to any, please give details below.  
I declare that I have no competing interests.  
I agree to the open peer review policy of the journal . I understand that my name will be included 
on my report to the authors and, if the manuscript is accepted for publication, my named report 
including any attachments I upload will be posted on the website along with the authors' 
responses. I agree for my report to be made available under an Open Acc ess Creative Commons 
CC-BY license ( http://creativecommons.org/licenses/by/4.0/ ). I understand that any comments 
which I do not wish to be included in my named report can be included as confidential comments 
to the editors, which will not be published.  
I agree to the open peer review policy of the journal  
Please review this paper.
user
Reviewer ’s report  
Title: The gut microbiome as a target for regulatory T cell -based immunotherapy: Induction of  
regulatory lymphocytes by oral administration of anti -LPS enriched colostrum alleviates immune  
mediated colitis  
 
Version: 0 Date: 19 Sep 2015  
Reviewer: Chen Shuan Chung  
Reviewer's report:  
It is an interesting study using oral  administration  of   Imm124E -induced colostrum to 
investigate the immune reponse in colitic mice model. However, there are some comments to this 
study:  
 
Major:  
1. The number of mice in each group is too small to draw a conclusion to the result since 
that some data did not sh ow significant difference between groups but only trends.  
 
2. In the method part, please describe the grading system for damage and regeneration 
scores in details.  
 
Minor:  
1. page 4 Grading of colon histology: There are some non -English letters at the endin g of 
the paragraph.  
 
2. page 3 line 50 Imm124Einduced -> Imm124E -induced  
 
3. page 4 line 25 mice were purchase -> mice were purchased  
 
4. page 5 line 15 4oC -> should be superscript character for "o".  
 
5. page 6 line 16,29,30 significantly and significant were  marked, please describe it why?  
 
6. page 9 line 7 Imm124Einduced -> imm124E induced  
Are the methods appropriate and well described?  
If not, please specify what is required in your comments to the authors.  
No 
Does the work include the necessary controls?  
If not, please specify which controls are re quired in your comments to the authors.  
Yes 
Are the conclusions drawn adequately supported by the data shown?  
If not, please explain in your comments to the authors.  
Unable to assess  
Are you able to assess any statistics in the manuscript or would you recommen d an 
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects require further  
assessment in your comments to the editors.  
I am able to assess the statistics  
Quality of written En glish  
Please indicate the quality of language in the manuscript:  
Acceptable  
Declaration of competing interests  
Please complete a declaration of competing interests, considering the following questions:  
1. Have you in the past five years received reimbursements, fees, funding, or salary from an 
organisation that may in any way gain or lose financially from the publication of this 
manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares in an organisation that may in any way gain or lose 
financially from the publication of this manuscript, either now or in th e future?  
 
3. Do you hold or are you currently applying for any patents relating to the content of the 
manuscript?  
 
4. Have you received reimbursements, fees, funding, or salary from an organization that 
holds or has applied for patents relating to the content of the manuscript?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have any non -financial competing interests in relation to this paper?  
If you can answer no to all of the above, write 'I declare that I have no competing interests' 
below. If your reply is yes to any, please give details below.  
I declare that I have no competing interests.  
I agree to the open peer review policy of the journal . I understand that my name will be included 
on my report to the authors and, if the manuscript is accepted for publication, my named report 
including any attachments I upload will be posted on the website along with the authors' 
responses. I agree for my report to be made available under an Open Acc ess Creative Commons 
CC-BY license ( http://creativecommons.org/licenses/by/4.0/ ). I understand that any comments 
which I do not wish to be included in my named report can be included as confidential comments 
to the editors, which will not be published.  
I agree to the open peer review policy of the journal  
assistant