Title: Nuclear respiratory factor 1 regulates super enhancer-controlled SPIDR to protect
hepatocellular carcinoma cells from oxidative stress
Version: 0 Date: 28 Oct 2023
Reviewer's report:
Authors tried to investigate the role and mechanism of super enhancer (SE)-controlled genes in
HCC cells response to oxidative stress. Authors described a  mechanism that NRF1 trigger SE-
controlled SPIDR protect hepatocellular carcinoma cells from oxidative stress. In some aspect,
it is a novelty. However, some major concerns should be elucidated.
1. Lack of some important figures, such as ROS change after the knock-down of SPIDR.
2. Add some experiments such as the chemical probe  indicates the change of ROS and
mitochondria after the exposure to H2O2.
Title: Nuclear respiratory factor 1 regulates super enhancer-controlled SPIDR to protect
hepatocellular carcinoma cells from oxidative stress
Version: 0 Date: 28 Oct 2023
Reviewer's report:
Authors tried to investigate the role and mechanism of super enhancer (SE)-controlled genes in
HCC cells response to oxidative stress. Authors described a  mechanism that NRF1 trigger SE-
controlled SPIDR protect hepatocellular carcinoma cells from oxidative stress. In some aspect,
it is a novelty. However, some major concerns should be elucidated.
1. Lack of some important figures, such as ROS change after the knock-down of SPIDR.
2. Add some experiments such as the chemical probe  indicates the change of ROS and
mitochondria after the exposure to H2O2.
Please review this paper.
user
Title: Nuclear respiratory factor 1 regulates super enhancer-controlled SPIDR to protect
hepatocellular carcinoma cells from oxidative stress
Version: 0 Date: 28 Oct 2023
Reviewer's report:
Authors tried to investigate the role and mechanism of super enhancer (SE)-controlled genes in
HCC cells response to oxidative stress. Authors described a  mechanism that NRF1 trigger SE-
controlled SPIDR protect hepatocellular carcinoma cells from oxidative stress. In some aspect,
it is a novelty. However, some major concerns should be elucidated.
1. Lack of some important figures, such as ROS change after the knock-down of SPIDR.
2. Add some experiments such as the chemical probe  indicates the change of ROS and
mitochondria after the exposure to H2O2.
assistant