Reviewer's report
Title:Deregulated expression of Nucleophosmin 1 in gastric cancer and its
clinicopathological implications
Version: 2Date:21 August 2013
Reviewer: Maria Piazuelo
Reviewer's report:
Immunohistochemistry studies for NPM1 are incomplete and the number of
samples used is even smaller (n=12 tumors), providing unclear results. There is
no data supporting IHC evaluation in non-tumoral and/or normal gastric mucosa.
However, in Table 2, which includes only tumors, case #5 shows score for
staining in oxyntic cells.
There is no comment on the correlation between NPM1 expression by Western
blot and IHC.
Again, the authors conclude that they have “demonstrated that nucleophosmin
may act as a tumor suppressor in gastric carcinogenesis, especially in
intestinal-type GC and in tumors from patients with distant metastasis”. The small
sample size and the results presented do not support this conclusion.
Minor comment: In second paragraph of Background section it should read NO38
instead of NO28.
Level of interest: An article of limited interest
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:Deregulated expression of Nucleophosmin 1 in gastric cancer and its
clinicopathological implications
Version: 2Date:21 August 2013
Reviewer: Maria Piazuelo
Reviewer's report:
Immunohistochemistry studies for NPM1 are incomplete and the number of
samples used is even smaller (n=12 tumors), providing unclear results. There is
no data supporting IHC evaluation in non-tumoral and/or normal gastric mucosa.
However, in Table 2, which includes only tumors, case #5 shows score for
staining in oxyntic cells.
There is no comment on the correlation between NPM1 expression by Western
blot and IHC.
Again, the authors conclude that they have “demonstrated that nucleophosmin
may act as a tumor suppressor in gastric carcinogenesis, especially in
intestinal-type GC and in tumors from patients with distant metastasis”. The small
sample size and the results presented do not support this conclusion.
Minor comment: In second paragraph of Background section it should read NO38
instead of NO28.
Level of interest: An article of limited interest
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:Deregulated expression of Nucleophosmin 1 in gastric cancer and its
clinicopathological implications
Version: 2Date:21 August 2013
Reviewer: Maria Piazuelo
Reviewer's report:
Immunohistochemistry studies for NPM1 are incomplete and the number of
samples used is even smaller (n=12 tumors), providing unclear results. There is
no data supporting IHC evaluation in non-tumoral and/or normal gastric mucosa.
However, in Table 2, which includes only tumors, case #5 shows score for
staining in oxyntic cells.
There is no comment on the correlation between NPM1 expression by Western
blot and IHC.
Again, the authors conclude that they have “demonstrated that nucleophosmin
may act as a tumor suppressor in gastric carcinogenesis, especially in
intestinal-type GC and in tumors from patients with distant metastasis”. The small
sample size and the results presented do not support this conclusion.
Minor comment: In second paragraph of Background section it should read NO38
instead of NO28.
Level of interest: An article of limited interest
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests.
assistant