Title: Evaluation of vital genes correlated with CD8+ T cell infiltration as prognostic biomarkers
in stomach adenocarcinoma
Version: 4 Date: 16 Sep 2023
Reviewer's report:
Additional experiments have further clarified the impact of this risk score model using vital 4
genes on STAD prognosis, and thus I read this part with great interest. However, this paper
includes a major problem especially for the evaluation of  CD8+ TILs that cannot be ignored,
and as a result, the clinical part holds only limited meaning.
1. Please perform immunohistochemical (IHC) analysis about CD8+ TILs using STAD resected
samples in your institution to evaluate the association between risk score and the extent of CD8+
TILs. I  think it's not time-consuming  process.
To describe that "the risk score was positively correlated with CD8+ T cell infiltration" in
abstract, this is essential experiment.
2.Please discuss the supposed mechanisms about the correlation between these four genes and
the infiltration of CD8+ T cells into the tumor, rather than just stating that a previous report
exists.
3. The chemotherapies usually used in gastric cancer are fluorouracila , oxaliplatin and cisplatin.
Please evaluate the relationship between risk score and IC50 of  these three drugs.
I hope these comments will be helpful.
Title: Evaluation of vital genes correlated with CD8+ T cell infiltration as prognostic biomarkers
in stomach adenocarcinoma
Version: 4 Date: 16 Sep 2023
Reviewer's report:
Additional experiments have further clarified the impact of this risk score model using vital 4
genes on STAD prognosis, and thus I read this part with great interest. However, this paper
includes a major problem especially for the evaluation of  CD8+ TILs that cannot be ignored,
and as a result, the clinical part holds only limited meaning.
1. Please perform immunohistochemical (IHC) analysis about CD8+ TILs using STAD resected
samples in your institution to evaluate the association between risk score and the extent of CD8+
TILs. I  think it's not time-consuming  process.
To describe that "the risk score was positively correlated with CD8+ T cell infiltration" in
abstract, this is essential experiment.
2.Please discuss the supposed mechanisms about the correlation between these four genes and
the infiltration of CD8+ T cells into the tumor, rather than just stating that a previous report
exists.
3. The chemotherapies usually used in gastric cancer are fluorouracila , oxaliplatin and cisplatin.
Please evaluate the relationship between risk score and IC50 of  these three drugs.
I hope these comments will be helpful.
Please review this paper.
user
Title: Evaluation of vital genes correlated with CD8+ T cell infiltration as prognostic biomarkers
in stomach adenocarcinoma
Version: 4 Date: 16 Sep 2023
Reviewer's report:
Additional experiments have further clarified the impact of this risk score model using vital 4
genes on STAD prognosis, and thus I read this part with great interest. However, this paper
includes a major problem especially for the evaluation of  CD8+ TILs that cannot be ignored,
and as a result, the clinical part holds only limited meaning.
1. Please perform immunohistochemical (IHC) analysis about CD8+ TILs using STAD resected
samples in your institution to evaluate the association between risk score and the extent of CD8+
TILs. I  think it's not time-consuming  process.
To describe that "the risk score was positively correlated with CD8+ T cell infiltration" in
abstract, this is essential experiment.
2.Please discuss the supposed mechanisms about the correlation between these four genes and
the infiltration of CD8+ T cells into the tumor, rather than just stating that a previous report
exists.
3. The chemotherapies usually used in gastric cancer are fluorouracila , oxaliplatin and cisplatin.
Please evaluate the relationship between risk score and IC50 of  these three drugs.
I hope these comments will be helpful.
assistant