Title: Prognostic and Therapeutic Potential of CXCR6 Expression on CD8+ T Cells in Gastric
Cancer: A Retrospective Cohort Study
Version: 0 Date: 23 Dec 2024
Reviewer's report:
By bioinformatics analysis, the authors uncovered the clinical significance of CD8+ T
cell infiltration in GC patients, as well as found that CXCR6 was mostly expressed in
CD8+ T cells and was clinically significant for GC patients as well as related to an
immunoreactive microenvironment. Finally, CXCR6 expression in immune cells was verified
by immunohistochemistry, and this expression is related to prognosis in GC patients. The
following are specific comments.
1. In the Results section, the authors demonstrated that “Particularly, the grey module showed
a high correlation with the CD8H group (r = 0.65, P < 0.001), and 42 hub genes were identified
within this module.” It is suggested that the 42 hub genes be listed in a table.
2. In the Results section, the authors demonstrated that “Using univariate Cox regression
analysis, we identified CXCR6 as a prognostic gene for GC (P < 0.05) (Supplementary Fig.
2).” What about the association between the remaining 41 hub genes and the prognosis of GC
patients? The authors should clarify why they chose CXCR6 for subsequent study.
3. Figure 6, the conclusion would be more solid if the authors could confirm that CXCR6 is
predominantly expressed in CD8+ T cells in the local patient samples.
4. The authors mentioned Supplementary Table 1 and Supplementary Table 2 in the manuscript,
however they are not available. Please check and provide.
5. According to the manuscript description, Figs. 5b and 5c appear to be in reverse. Please check
and correct.
6. Figures 2 and 6, it is suggested that the survival types be indicated in the images.
7. Some method descriptions in “Analysis of single-cell RNA sequencing data for CXCR6
expression” and “Immunotherapy response” are repeated.
Title: Prognostic and Therapeutic Potential of CXCR6 Expression on CD8+ T Cells in Gastric
Cancer: A Retrospective Cohort Study
Version: 0 Date: 23 Dec 2024
Reviewer's report:
By bioinformatics analysis, the authors uncovered the clinical significance of CD8+ T
cell infiltration in GC patients, as well as found that CXCR6 was mostly expressed in
CD8+ T cells and was clinically significant for GC patients as well as related to an
immunoreactive microenvironment. Finally, CXCR6 expression in immune cells was verified
by immunohistochemistry, and this expression is related to prognosis in GC patients. The
following are specific comments.
1. In the Results section, the authors demonstrated that “Particularly, the grey module showed
a high correlation with the CD8H group (r = 0.65, P < 0.001), and 42 hub genes were identified
within this module.” It is suggested that the 42 hub genes be listed in a table.
2. In the Results section, the authors demonstrated that “Using univariate Cox regression
analysis, we identified CXCR6 as a prognostic gene for GC (P < 0.05) (Supplementary Fig.
2).” What about the association between the remaining 41 hub genes and the prognosis of GC
patients? The authors should clarify why they chose CXCR6 for subsequent study.
3. Figure 6, the conclusion would be more solid if the authors could confirm that CXCR6 is
predominantly expressed in CD8+ T cells in the local patient samples.
4. The authors mentioned Supplementary Table 1 and Supplementary Table 2 in the manuscript,
however they are not available. Please check and provide.
5. According to the manuscript description, Figs. 5b and 5c appear to be in reverse. Please check
and correct.
6. Figures 2 and 6, it is suggested that the survival types be indicated in the images.
7. Some method descriptions in “Analysis of single-cell RNA sequencing data for CXCR6
expression” and “Immunotherapy response” are repeated.
Please review this paper.
user
Title: Prognostic and Therapeutic Potential of CXCR6 Expression on CD8+ T Cells in Gastric
Cancer: A Retrospective Cohort Study
Version: 0 Date: 23 Dec 2024
Reviewer's report:
By bioinformatics analysis, the authors uncovered the clinical significance of CD8+ T
cell infiltration in GC patients, as well as found that CXCR6 was mostly expressed in
CD8+ T cells and was clinically significant for GC patients as well as related to an
immunoreactive microenvironment. Finally, CXCR6 expression in immune cells was verified
by immunohistochemistry, and this expression is related to prognosis in GC patients. The
following are specific comments.
1. In the Results section, the authors demonstrated that “Particularly, the grey module showed
a high correlation with the CD8H group (r = 0.65, P < 0.001), and 42 hub genes were identified
within this module.” It is suggested that the 42 hub genes be listed in a table.
2. In the Results section, the authors demonstrated that “Using univariate Cox regression
analysis, we identified CXCR6 as a prognostic gene for GC (P < 0.05) (Supplementary Fig.
2).” What about the association between the remaining 41 hub genes and the prognosis of GC
patients? The authors should clarify why they chose CXCR6 for subsequent study.
3. Figure 6, the conclusion would be more solid if the authors could confirm that CXCR6 is
predominantly expressed in CD8+ T cells in the local patient samples.
4. The authors mentioned Supplementary Table 1 and Supplementary Table 2 in the manuscript,
however they are not available. Please check and provide.
5. According to the manuscript description, Figs. 5b and 5c appear to be in reverse. Please check
and correct.
6. Figures 2 and 6, it is suggested that the survival types be indicated in the images.
7. Some method descriptions in “Analysis of single-cell RNA sequencing data for CXCR6
expression” and “Immunotherapy response” are repeated.
assistant