Reviewer's report
Title:TNF-alpha-induced up-regulation of pro-inflammatory cytokines is reduced
by phosphatidylcholine in intestinal epithelial cells
Version: 4Date:11 March 2009
Reviewer: Eva Rath
Reviewer's report:
Too few new data to publish:
Figure 1 A: effect shown in a different readout
see article * Figure 5C
Figure 1B: similar data already published;
see article * Figure 5D: (difference: PC 18:2)
Figure 2: time course of 1B, ok
Figure 3: similar data already published;
see article * Table1: (4%<->95%?)
Figure 4: similar data already published;
see article * Figure 6A: (new: MMP-1, t=240min)
Figure 5: ok; MCP-1: TNF+SN50 higher induction than TNF after 120min?
ICAM-1: TNF+SN50 and TNF: similar induction after 120min?
Figure 6: PC do not inhibit TNF binding to its receptor; ok
Figure 7: Lipid raft association:
“PC and LPC induced also an increased DRM association for the raft marker
proteins Yes and Flotillin.1 (data not shown) that served as controls” -> not TNF
Receptor specific effect
Figure 8: apical/basolateral TNF stimulation;
known effect in polarized cells
Figure 9: PC effective for apical & basolateral side; ok
*Anti-inflammatory effects of phosphatidylcholine.
Treede I, Braun A, Sparla R, Kühnel M, Giese T, Turner JR, Anes E, Kulaksiz H,
Füllekrug J, Stremmel W, Griffiths G, Ehehalt R.
J Biol Chem. 2007 Sep 14;282(37):27155-64. Epub 2007 Jul 18.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:TNF-alpha-induced up-regulation of pro-inflammatory cytokines is reduced
by phosphatidylcholine in intestinal epithelial cells
Version: 4Date:11 March 2009
Reviewer: Eva Rath
Reviewer's report:
Too few new data to publish:
Figure 1 A: effect shown in a different readout
see article * Figure 5C
Figure 1B: similar data already published;
see article * Figure 5D: (difference: PC 18:2)
Figure 2: time course of 1B, ok
Figure 3: similar data already published;
see article * Table1: (4%<->95%?)
Figure 4: similar data already published;
see article * Figure 6A: (new: MMP-1, t=240min)
Figure 5: ok; MCP-1: TNF+SN50 higher induction than TNF after 120min?
ICAM-1: TNF+SN50 and TNF: similar induction after 120min?
Figure 6: PC do not inhibit TNF binding to its receptor; ok
Figure 7: Lipid raft association:
“PC and LPC induced also an increased DRM association for the raft marker
proteins Yes and Flotillin.1 (data not shown) that served as controls” -> not TNF
Receptor specific effect
Figure 8: apical/basolateral TNF stimulation;
known effect in polarized cells
Figure 9: PC effective for apical & basolateral side; ok
*Anti-inflammatory effects of phosphatidylcholine.
Treede I, Braun A, Sparla R, Kühnel M, Giese T, Turner JR, Anes E, Kulaksiz H,
Füllekrug J, Stremmel W, Griffiths G, Ehehalt R.
J Biol Chem. 2007 Sep 14;282(37):27155-64. Epub 2007 Jul 18.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:TNF-alpha-induced up-regulation of pro-inflammatory cytokines is reduced
by phosphatidylcholine in intestinal epithelial cells
Version: 4Date:11 March 2009
Reviewer: Eva Rath
Reviewer's report:
Too few new data to publish:
Figure 1 A: effect shown in a different readout
see article * Figure 5C
Figure 1B: similar data already published;
see article * Figure 5D: (difference: PC 18:2)
Figure 2: time course of 1B, ok
Figure 3: similar data already published;
see article * Table1: (4%<->95%?)
Figure 4: similar data already published;
see article * Figure 6A: (new: MMP-1, t=240min)
Figure 5: ok; MCP-1: TNF+SN50 higher induction than TNF after 120min?
ICAM-1: TNF+SN50 and TNF: similar induction after 120min?
Figure 6: PC do not inhibit TNF binding to its receptor; ok
Figure 7: Lipid raft association:
“PC and LPC induced also an increased DRM association for the raft marker
proteins Yes and Flotillin.1 (data not shown) that served as controls” -> not TNF
Receptor specific effect
Figure 8: apical/basolateral TNF stimulation;
known effect in polarized cells
Figure 9: PC effective for apical & basolateral side; ok
*Anti-inflammatory effects of phosphatidylcholine.
Treede I, Braun A, Sparla R, Kühnel M, Giese T, Turner JR, Anes E, Kulaksiz H,
Füllekrug J, Stremmel W, Griffiths G, Ehehalt R.
J Biol Chem. 2007 Sep 14;282(37):27155-64. Epub 2007 Jul 18.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
Declaration of competing interests:
I declare that I have no competing interests
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