Title: Overexpression of Protein Kinase C iota as a Prognostic Marker and Its Role in Oxaliplatin
Resistance in colorectal Cancer
Version: 3 Date: 25 May 2025
Reviewer's report:
This study investigated the expression of PKC# in colorectal cancer (CRC) and its association
with clinicopathological features, prognosis, and chemotherapy resistance. The research design
is reasonable, the data are substantial, and the conclusions hold certain clinical significance. The
article is well-structured, with detailed methodological descriptions and comprehensive results
analysis. However, some details require further refinement and clarification.
1. Only 42 patients were included, which may affect the statistical power of the results. It is
recommended to mention the limitation of sample size in the Discussion section and suggest
future validation with a larger cohort.
2. In the multivariate analysis, PKC# did not emerge as an independent prognostic factor, yet
the conclusion still emphasizes its importance. It is advisable to discuss this discrepancy more
cautiously or explore how PKC# may indirectly influence prognosis through other factors.
3. Some figures (e.g., Fig. 1) lack detailed explanations in the text. It is recommended to
supplement specific data or descriptions of representative images.
4. The discussion could be deepened by further exploring the specific mechanisms by which
PKC# mediates chemotherapy resistance (e.g., DNA damage repair, apoptosis inhibition, etc.)
to enhance scientific rigor.
5. Full terms should be provided upon first use of abbreviations (e.g., PFS, OS).
Title: Overexpression of Protein Kinase C iota as a Prognostic Marker and Its Role in Oxaliplatin
Resistance in colorectal Cancer
Version: 3 Date: 25 May 2025
Reviewer's report:
This study investigated the expression of PKC# in colorectal cancer (CRC) and its association
with clinicopathological features, prognosis, and chemotherapy resistance. The research design
is reasonable, the data are substantial, and the conclusions hold certain clinical significance. The
article is well-structured, with detailed methodological descriptions and comprehensive results
analysis. However, some details require further refinement and clarification.
1. Only 42 patients were included, which may affect the statistical power of the results. It is
recommended to mention the limitation of sample size in the Discussion section and suggest
future validation with a larger cohort.
2. In the multivariate analysis, PKC# did not emerge as an independent prognostic factor, yet
the conclusion still emphasizes its importance. It is advisable to discuss this discrepancy more
cautiously or explore how PKC# may indirectly influence prognosis through other factors.
3. Some figures (e.g., Fig. 1) lack detailed explanations in the text. It is recommended to
supplement specific data or descriptions of representative images.
4. The discussion could be deepened by further exploring the specific mechanisms by which
PKC# mediates chemotherapy resistance (e.g., DNA damage repair, apoptosis inhibition, etc.)
to enhance scientific rigor.
5. Full terms should be provided upon first use of abbreviations (e.g., PFS, OS).
Please review this paper.
user
Title: Overexpression of Protein Kinase C iota as a Prognostic Marker and Its Role in Oxaliplatin
Resistance in colorectal Cancer
Version: 3 Date: 25 May 2025
Reviewer's report:
This study investigated the expression of PKC# in colorectal cancer (CRC) and its association
with clinicopathological features, prognosis, and chemotherapy resistance. The research design
is reasonable, the data are substantial, and the conclusions hold certain clinical significance. The
article is well-structured, with detailed methodological descriptions and comprehensive results
analysis. However, some details require further refinement and clarification.
1. Only 42 patients were included, which may affect the statistical power of the results. It is
recommended to mention the limitation of sample size in the Discussion section and suggest
future validation with a larger cohort.
2. In the multivariate analysis, PKC# did not emerge as an independent prognostic factor, yet
the conclusion still emphasizes its importance. It is advisable to discuss this discrepancy more
cautiously or explore how PKC# may indirectly influence prognosis through other factors.
3. Some figures (e.g., Fig. 1) lack detailed explanations in the text. It is recommended to
supplement specific data or descriptions of representative images.
4. The discussion could be deepened by further exploring the specific mechanisms by which
PKC# mediates chemotherapy resistance (e.g., DNA damage repair, apoptosis inhibition, etc.)
to enhance scientific rigor.
5. Full terms should be provided upon first use of abbreviations (e.g., PFS, OS).
assistant