Title: Increased expression of NOP14 is associated with improved prognosis due to immune
regulation in colorectal cancer
Version: 1 Date: 14 Mar 2022
Reviewer's report:
This manuscript from Lu et al., concerns NOP14 and its relationship with CRC and other cancer
types. The authors use publicly available data to provide some evidence that in CRC, NOP14
over-expression indicates a better prognosis. It is a well constructed paper with some interesting
results. Below are some comments on the manuscript:
Introduction
- It’s important to make note that the 5-yr survival rate for CRC is for the Chinese population.
Materials & Methods
- Unfortunately, I am unable to critique the bioinformatic methods put forward in this
manuscript, as it is outside my expertise.
- How did the authors come to having exactly 322 low expression tumors AND high
expression tumors. This isn’t explained, and is it assumed you identified these and picked them
purposefully? If so, were there any other parameters for picking these particular tumors?
- When the authors assessed NOP14 protein expression via IHC, were any of these images from
the same subjects that were used for the gene expression analyses? If so, please emphasize. If
not, please describe how this may be a weakness in the methodology.
Discussion
- In the second sentence of this section, there are words missing after “requiring”.
- The 4th sentence indicates that the authors were examining multiple genes for expression in
tumor cells, but NOP14 seems to be the only one studied directly in the manuscript.
- This sentence has no reference. Please include references. “It has been recently reported that
NOP14 can be considered an immune regulator factor, and that it is involved in various diseases.”
- The concept of having a Figure 10 is important, however the current iteration of the figure
does not provide much information, particularly since NOP14 is basically “floating” around in
the nucleus rather ambiguously.
Other
- In table 2, “over survival” is not a used phrase. “Survival” should be fine.
- There are over 2 dozen grammatical errors including missing words and run-on sentences that
need to be addressed.
Title: Increased expression of NOP14 is associated with improved prognosis due to immune
regulation in colorectal cancer
Version: 1 Date: 14 Mar 2022
Reviewer's report:
This manuscript from Lu et al., concerns NOP14 and its relationship with CRC and other cancer
types. The authors use publicly available data to provide some evidence that in CRC, NOP14
over-expression indicates a better prognosis. It is a well constructed paper with some interesting
results. Below are some comments on the manuscript:
Introduction
- It’s important to make note that the 5-yr survival rate for CRC is for the Chinese population.
Materials & Methods
- Unfortunately, I am unable to critique the bioinformatic methods put forward in this
manuscript, as it is outside my expertise.
- How did the authors come to having exactly 322 low expression tumors AND high
expression tumors. This isn’t explained, and is it assumed you identified these and picked them
purposefully? If so, were there any other parameters for picking these particular tumors?
- When the authors assessed NOP14 protein expression via IHC, were any of these images from
the same subjects that were used for the gene expression analyses? If so, please emphasize. If
not, please describe how this may be a weakness in the methodology.
Discussion
- In the second sentence of this section, there are words missing after “requiring”.
- The 4th sentence indicates that the authors were examining multiple genes for expression in
tumor cells, but NOP14 seems to be the only one studied directly in the manuscript.
- This sentence has no reference. Please include references. “It has been recently reported that
NOP14 can be considered an immune regulator factor, and that it is involved in various diseases.”
- The concept of having a Figure 10 is important, however the current iteration of the figure
does not provide much information, particularly since NOP14 is basically “floating” around in
the nucleus rather ambiguously.
Other
- In table 2, “over survival” is not a used phrase. “Survival” should be fine.
- There are over 2 dozen grammatical errors including missing words and run-on sentences that
need to be addressed.
Please review this paper.
user
Title: Increased expression of NOP14 is associated with improved prognosis due to immune
regulation in colorectal cancer
Version: 1 Date: 14 Mar 2022
Reviewer's report:
This manuscript from Lu et al., concerns NOP14 and its relationship with CRC and other cancer
types. The authors use publicly available data to provide some evidence that in CRC, NOP14
over-expression indicates a better prognosis. It is a well constructed paper with some interesting
results. Below are some comments on the manuscript:
Introduction
- It’s important to make note that the 5-yr survival rate for CRC is for the Chinese population.
Materials & Methods
- Unfortunately, I am unable to critique the bioinformatic methods put forward in this
manuscript, as it is outside my expertise.
- How did the authors come to having exactly 322 low expression tumors AND high
expression tumors. This isn’t explained, and is it assumed you identified these and picked them
purposefully? If so, were there any other parameters for picking these particular tumors?
- When the authors assessed NOP14 protein expression via IHC, were any of these images from
the same subjects that were used for the gene expression analyses? If so, please emphasize. If
not, please describe how this may be a weakness in the methodology.
Discussion
- In the second sentence of this section, there are words missing after “requiring”.
- The 4th sentence indicates that the authors were examining multiple genes for expression in
tumor cells, but NOP14 seems to be the only one studied directly in the manuscript.
- This sentence has no reference. Please include references. “It has been recently reported that
NOP14 can be considered an immune regulator factor, and that it is involved in various diseases.”
- The concept of having a Figure 10 is important, however the current iteration of the figure
does not provide much information, particularly since NOP14 is basically “floating” around in
the nucleus rather ambiguously.
Other
- In table 2, “over survival” is not a used phrase. “Survival” should be fine.
- There are over 2 dozen grammatical errors including missing words and run-on sentences that
need to be addressed.
assistant