Title: Nuclear respiratory factor 1 promotes the growth of liver hepatocellular carcinoma cells
via E2F1 transcriptional activation
Version: 5 Date: 31 Mar 2022
Reviewer's report:
In this manuscript, the authors found the NRF1 expression was associated with tumour size
and poor prognosis in patients. Besides, they also discovered the downstream target genes of
NRF1 through CHIP, and found NRF1 directly binds to the E2F1 promoter as a transcription
factor and regulates  its cognate gene expression profile. Overall,  almost all questions raised
by previous Reviewers have been addressed in this revised version of this paper, which should
thus be acceptable for publication in this journal.
Title: Nuclear respiratory factor 1 promotes the growth of liver hepatocellular carcinoma cells
via E2F1 transcriptional activation
Version: 5 Date: 31 Mar 2022
Reviewer's report:
In this manuscript, the authors found the NRF1 expression was associated with tumour size
and poor prognosis in patients. Besides, they also discovered the downstream target genes of
NRF1 through CHIP, and found NRF1 directly binds to the E2F1 promoter as a transcription
factor and regulates  its cognate gene expression profile. Overall,  almost all questions raised
by previous Reviewers have been addressed in this revised version of this paper, which should
thus be acceptable for publication in this journal.
Please review this paper.
user
Title: Nuclear respiratory factor 1 promotes the growth of liver hepatocellular carcinoma cells
via E2F1 transcriptional activation
Version: 5 Date: 31 Mar 2022
Reviewer's report:
In this manuscript, the authors found the NRF1 expression was associated with tumour size
and poor prognosis in patients. Besides, they also discovered the downstream target genes of
NRF1 through CHIP, and found NRF1 directly binds to the E2F1 promoter as a transcription
factor and regulates  its cognate gene expression profile. Overall,  almost all questions raised
by previous Reviewers have been addressed in this revised version of this paper, which should
thus be acceptable for publication in this journal.
assistant