Reviewer's report
Title:Transglucosidase improves the gut microbiota profile of type 2 diabetes
mellitus patients
Version: 3Date:26 January 2013
Reviewer: Antonio Gasbarrini
Reviewer's report:
This is an interesting, well written paper assessing gut microbiota changes in
diabetes patients after the treatment with transglucosidase.
Major compulsory revisions
1 In the Subjects and Methods section the Auhtors stated that the enrollment
criteria and study design were previously described. Are the patients enrolled in
this study the same of reference 1? Please specify and add more details about
the study design and the enrollment criteria.
2 The Authors did not provide any explication about the evaluation of the sample
size, about the number oh healthy controls and about the choice of
tranglucosidase dosage. Please provide them.
3 The Authors should describe what kind of diabetes medication (including the
dosage) are used throughout the study.
4 In the Results section the Authors did not specify the difference in
Bacteroides/Firmicutes ratio between diabetic patients and healthy controls, but
in the Discussion section They stated that smaller Firmicutes populations were
observed in T2DM patients than in the healthy population. Please provide more
details.
5 The major limitation of this study is that the Authors dis not show any
correlation between gut microbiota modification and metabolic state of the
patients. Does modification of gut microbiota correlate with reduced levels of
HbA1c?
Minor essential revisions
6 Please provide a table showing individual results for any patients and not only
mean values and HbA1c levels before and after treatment.
Discretionary revisions
7 The abstract should specify how many healthy controls the study included
8 In the Subjects and Methods section the Authors stated that patients received
treatment after every meal. Please specify that patients received treatment 3
times a day after main meals.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Transglucosidase improves the gut microbiota profile of type 2 diabetes
mellitus patients
Version: 3Date:26 January 2013
Reviewer: Antonio Gasbarrini
Reviewer's report:
This is an interesting, well written paper assessing gut microbiota changes in
diabetes patients after the treatment with transglucosidase.
Major compulsory revisions
1 In the Subjects and Methods section the Auhtors stated that the enrollment
criteria and study design were previously described. Are the patients enrolled in
this study the same of reference 1? Please specify and add more details about
the study design and the enrollment criteria.
2 The Authors did not provide any explication about the evaluation of the sample
size, about the number oh healthy controls and about the choice of
tranglucosidase dosage. Please provide them.
3 The Authors should describe what kind of diabetes medication (including the
dosage) are used throughout the study.
4 In the Results section the Authors did not specify the difference in
Bacteroides/Firmicutes ratio between diabetic patients and healthy controls, but
in the Discussion section They stated that smaller Firmicutes populations were
observed in T2DM patients than in the healthy population. Please provide more
details.
5 The major limitation of this study is that the Authors dis not show any
correlation between gut microbiota modification and metabolic state of the
patients. Does modification of gut microbiota correlate with reduced levels of
HbA1c?
Minor essential revisions
6 Please provide a table showing individual results for any patients and not only
mean values and HbA1c levels before and after treatment.
Discretionary revisions
7 The abstract should specify how many healthy controls the study included
8 In the Subjects and Methods section the Authors stated that patients received
treatment after every meal. Please specify that patients received treatment 3
times a day after main meals.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Transglucosidase improves the gut microbiota profile of type 2 diabetes
mellitus patients
Version: 3Date:26 January 2013
Reviewer: Antonio Gasbarrini
Reviewer's report:
This is an interesting, well written paper assessing gut microbiota changes in
diabetes patients after the treatment with transglucosidase.
Major compulsory revisions
1 In the Subjects and Methods section the Auhtors stated that the enrollment
criteria and study design were previously described. Are the patients enrolled in
this study the same of reference 1? Please specify and add more details about
the study design and the enrollment criteria.
2 The Authors did not provide any explication about the evaluation of the sample
size, about the number oh healthy controls and about the choice of
tranglucosidase dosage. Please provide them.
3 The Authors should describe what kind of diabetes medication (including the
dosage) are used throughout the study.
4 In the Results section the Authors did not specify the difference in
Bacteroides/Firmicutes ratio between diabetic patients and healthy controls, but
in the Discussion section They stated that smaller Firmicutes populations were
observed in T2DM patients than in the healthy population. Please provide more
details.
5 The major limitation of this study is that the Authors dis not show any
correlation between gut microbiota modification and metabolic state of the
patients. Does modification of gut microbiota correlate with reduced levels of
HbA1c?
Minor essential revisions
6 Please provide a table showing individual results for any patients and not only
mean values and HbA1c levels before and after treatment.
Discretionary revisions
7 The abstract should specify how many healthy controls the study included
8 In the Subjects and Methods section the Authors stated that patients received
treatment after every meal. Please specify that patients received treatment 3
times a day after main meals.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
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