Title: Efficacy of extracorporeal immunoadsorption therapy for removal of HBsAg
Version: 3 Date: 02 Apr 2025
Reviewer's report:
As I discussed in the previous and original review, the authors could not provide a suitable
response to the queries related to the fundamental aspect of the article. Please follow the
comments regarding this.
1. The methodology of production of HBV-infected rabbit is not a rational one. Injecting HBsAg-
positive sera is not supposed to be an animal model of chronic HBV infection.
2. It has been mentioned that a rabbit model of viremia was produced by injecting HBsAg-
positive rabbit serum. I have considerable doubt about this. Additionally, this is not a rational
approach. In other words, a rabbit immunized by HBsAg is not supposed to show HBV DNA.
3. How did the rabbits exhibit HBV DNA? This is not possible in this type of model.
4. I am confused about the production of the model and the fundamental aspect of the article.
Title: Efficacy of extracorporeal immunoadsorption therapy for removal of HBsAg
Version: 3 Date: 02 Apr 2025
Reviewer's report:
As I discussed in the previous and original review, the authors could not provide a suitable
response to the queries related to the fundamental aspect of the article. Please follow the
comments regarding this.
1. The methodology of production of HBV-infected rabbit is not a rational one. Injecting HBsAg-
positive sera is not supposed to be an animal model of chronic HBV infection.
2. It has been mentioned that a rabbit model of viremia was produced by injecting HBsAg-
positive rabbit serum. I have considerable doubt about this. Additionally, this is not a rational
approach. In other words, a rabbit immunized by HBsAg is not supposed to show HBV DNA.
3. How did the rabbits exhibit HBV DNA? This is not possible in this type of model.
4. I am confused about the production of the model and the fundamental aspect of the article.
Please review this paper.
user
Title: Efficacy of extracorporeal immunoadsorption therapy for removal of HBsAg
Version: 3 Date: 02 Apr 2025
Reviewer's report:
As I discussed in the previous and original review, the authors could not provide a suitable
response to the queries related to the fundamental aspect of the article. Please follow the
comments regarding this.
1. The methodology of production of HBV-infected rabbit is not a rational one. Injecting HBsAg-
positive sera is not supposed to be an animal model of chronic HBV infection.
2. It has been mentioned that a rabbit model of viremia was produced by injecting HBsAg-
positive rabbit serum. I have considerable doubt about this. Additionally, this is not a rational
approach. In other words, a rabbit immunized by HBsAg is not supposed to show HBV DNA.
3. How did the rabbits exhibit HBV DNA? This is not possible in this type of model.
4. I am confused about the production of the model and the fundamental aspect of the article.
assistant