Title: The hsa_circ_0039857/miR-338-3p/RAB32 axis promotes the malignant progression of
colorectal cancer
Version: 4 Date: 16 Nov 2022
Reviewer's report:
The sequence of siRNAs for circRNA was not provided.  Please provide the sequence in the
material and method section.
The real-time PCR  method for miRNA was so ambiguous. Did the authors use stem-loop
method? Please provide the RT primer for U6 and miRNA.
in the sentence "Interaction of circRNA-miRNA was predicted by the StarBase and circBase
online databases. " please mention the web page addresses.
The sentence "Target regions of miRNA-mRNA were predicted by StarBase and miRBase
bioinformatics tools. RAB32 was a predicted target of miR-338-3p." should be checked did you
mean mirdb instead of miRBase. please mention the related web page dresses.
There was some grammatical and writing error that should be revised.
In table 2 please provide the annealing temperature of primers
Title: The hsa_circ_0039857/miR-338-3p/RAB32 axis promotes the malignant progression of
colorectal cancer
Version: 4 Date: 16 Nov 2022
Reviewer's report:
The sequence of siRNAs for circRNA was not provided.  Please provide the sequence in the
material and method section.
The real-time PCR  method for miRNA was so ambiguous. Did the authors use stem-loop
method? Please provide the RT primer for U6 and miRNA.
in the sentence "Interaction of circRNA-miRNA was predicted by the StarBase and circBase
online databases. " please mention the web page addresses.
The sentence "Target regions of miRNA-mRNA were predicted by StarBase and miRBase
bioinformatics tools. RAB32 was a predicted target of miR-338-3p." should be checked did you
mean mirdb instead of miRBase. please mention the related web page dresses.
There was some grammatical and writing error that should be revised.
In table 2 please provide the annealing temperature of primers
Please review this paper.
user
Title: The hsa_circ_0039857/miR-338-3p/RAB32 axis promotes the malignant progression of
colorectal cancer
Version: 4 Date: 16 Nov 2022
Reviewer's report:
The sequence of siRNAs for circRNA was not provided.  Please provide the sequence in the
material and method section.
The real-time PCR  method for miRNA was so ambiguous. Did the authors use stem-loop
method? Please provide the RT primer for U6 and miRNA.
in the sentence "Interaction of circRNA-miRNA was predicted by the StarBase and circBase
online databases. " please mention the web page addresses.
The sentence "Target regions of miRNA-mRNA were predicted by StarBase and miRBase
bioinformatics tools. RAB32 was a predicted target of miR-338-3p." should be checked did you
mean mirdb instead of miRBase. please mention the related web page dresses.
There was some grammatical and writing error that should be revised.
In table 2 please provide the annealing temperature of primers
assistant