Reviewer's report
Title:Interrelationship between TP53 gene deletion, protein expression and
chromosome 17 aneusomy in gastric adenocarcinoma
Version: 1Date:7 April 2009
Reviewer: Tamotsu Sugai
Reviewer's report:
The author indicates that there are chr17 and TP53 copy number alterations in
gastric cancers. Although this manuscript is generally well written, several points
regarding the work are listed below:
1. The author analyzed 20 intestinal- and diffuse-type gastric cancers for
chromosome 17p and TP53 copy number alterations. However, the number of
tumor samples examined is too small to determine the role of 17p and TP53 copy
number alterations in gastric carcinogenesis. To examine the role of 17p and
TP53 copy number alterations in gastric carcinogenesis, the author should
increase the number of tumor samples.
2. Although the manuscript has some novel findings, in its present form the
author has not provided new findings about gastric carcinogenesis.
3. The author suggests that deletion of TP53 and chromosome 17 aneusomy are
common events in gastric cancer and that p53 mutation may be implicated in the
distinct carcinogenic process of diffuse- and intestinal-type cancers. However,
contrasting data were given. The author should discuss in the text the roles of
TP53 deletion, chromosome 17 aneusomy, and p53 mutation with regard to
intestinal- and diffuse-type cancers.
4. This study could not clarify the role of the above-mentioned alterations in the
early development of gastric cancers. The author should discuss this point in the
text.
5. In the Materials and Methods section the author should describe in detail the
FISH method that was used.
Level of interest: An article of limited interest
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests' below.
Reviewer's report
Title:Interrelationship between TP53 gene deletion, protein expression and
chromosome 17 aneusomy in gastric adenocarcinoma
Version: 1Date:7 April 2009
Reviewer: Tamotsu Sugai
Reviewer's report:
The author indicates that there are chr17 and TP53 copy number alterations in
gastric cancers. Although this manuscript is generally well written, several points
regarding the work are listed below:
1. The author analyzed 20 intestinal- and diffuse-type gastric cancers for
chromosome 17p and TP53 copy number alterations. However, the number of
tumor samples examined is too small to determine the role of 17p and TP53 copy
number alterations in gastric carcinogenesis. To examine the role of 17p and
TP53 copy number alterations in gastric carcinogenesis, the author should
increase the number of tumor samples.
2. Although the manuscript has some novel findings, in its present form the
author has not provided new findings about gastric carcinogenesis.
3. The author suggests that deletion of TP53 and chromosome 17 aneusomy are
common events in gastric cancer and that p53 mutation may be implicated in the
distinct carcinogenic process of diffuse- and intestinal-type cancers. However,
contrasting data were given. The author should discuss in the text the roles of
TP53 deletion, chromosome 17 aneusomy, and p53 mutation with regard to
intestinal- and diffuse-type cancers.
4. This study could not clarify the role of the above-mentioned alterations in the
early development of gastric cancers. The author should discuss this point in the
text.
5. In the Materials and Methods section the author should describe in detail the
FISH method that was used.
Level of interest: An article of limited interest
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests' below.
Please review this paper.
user
Reviewer's report
Title:Interrelationship between TP53 gene deletion, protein expression and
chromosome 17 aneusomy in gastric adenocarcinoma
Version: 1Date:7 April 2009
Reviewer: Tamotsu Sugai
Reviewer's report:
The author indicates that there are chr17 and TP53 copy number alterations in
gastric cancers. Although this manuscript is generally well written, several points
regarding the work are listed below:
1. The author analyzed 20 intestinal- and diffuse-type gastric cancers for
chromosome 17p and TP53 copy number alterations. However, the number of
tumor samples examined is too small to determine the role of 17p and TP53 copy
number alterations in gastric carcinogenesis. To examine the role of 17p and
TP53 copy number alterations in gastric carcinogenesis, the author should
increase the number of tumor samples.
2. Although the manuscript has some novel findings, in its present form the
author has not provided new findings about gastric carcinogenesis.
3. The author suggests that deletion of TP53 and chromosome 17 aneusomy are
common events in gastric cancer and that p53 mutation may be implicated in the
distinct carcinogenic process of diffuse- and intestinal-type cancers. However,
contrasting data were given. The author should discuss in the text the roles of
TP53 deletion, chromosome 17 aneusomy, and p53 mutation with regard to
intestinal- and diffuse-type cancers.
4. This study could not clarify the role of the above-mentioned alterations in the
early development of gastric cancers. The author should discuss this point in the
text.
5. In the Materials and Methods section the author should describe in detail the
FISH method that was used.
Level of interest: An article of limited interest
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests' below.
assistant