Title: Construction of a novel necroptosis-related lncRNA signature for prognosis prediction
in esophageal cancer
Version: 1 Date: 29 May 2022
Reviewer's report:
The research conducted by Yang Liu and colleagues under the title "Construction of a
novel necroptosis-related lncRNA signature for prognosis in esophageal cancer" shows the
significance in the scientific sense and future potential applications. The authors performed
the analysis by using a dataset of 171 samples obtained from the TCGA database with 160
esophageal cancer samples and 11 samples of normal esophageal tissue to seek necroptosis-
related lncRNAs as well as related genes in EC. Through multi-analysis using packages from
R software and others, the authors succeeded in building a predictive or prognostic model for
EC patients based on a group of biomarkers, including six necroptosis-associated lncRNAs.
The manuscript was also well prepared; therefore, I believe this study should be considered
for publication in the BMC Gastroenterology journal. However, before being published, the
following issues should be clarified to better convince readers, including:
1. The author should re-read the manuscript to correct the errors, for example, AUC value 536
on Page 10, line 222?
2. The author should revise Figure 1 to make the article more logical and understandable to
readers. For example, is the value 159 mentioned in Full-text?
3. In the supplementary section, the author should provide clinical information of 185 cases as
well as information on 171 samples used in this analysis.
4. Please consider using the term "correlation" instead of the expression "relationship/relation."
5. In Figure 7 about the nomogram, please explain more about the parameters. Commonly,
a nomogram should be used to quickly identify conditions (e.g., disease). Could the authors
explain how a clinician can quickly read the results from clinical tests using these six (06)
lncRNAs?
6. The study would be perfect and suitable for publication in the journal of BMC
Gastroenterology if at least one of these three (03) lncRNAs (Z94721.2, AL035461.2, and
AC051619.4) were verified experimentally. The author may consider performing a quick
quantitative qPCR analysis to verify the factual accuracy of this newly built model.
7. Finally, I strongly recommend that authors review the entire manuscript to revise the typos
and other related issues.
Title: Construction of a novel necroptosis-related lncRNA signature for prognosis prediction
in esophageal cancer
Version: 1 Date: 29 May 2022
Reviewer's report:
The research conducted by Yang Liu and colleagues under the title "Construction of a
novel necroptosis-related lncRNA signature for prognosis in esophageal cancer" shows the
significance in the scientific sense and future potential applications. The authors performed
the analysis by using a dataset of 171 samples obtained from the TCGA database with 160
esophageal cancer samples and 11 samples of normal esophageal tissue to seek necroptosis-
related lncRNAs as well as related genes in EC. Through multi-analysis using packages from
R software and others, the authors succeeded in building a predictive or prognostic model for
EC patients based on a group of biomarkers, including six necroptosis-associated lncRNAs.
The manuscript was also well prepared; therefore, I believe this study should be considered
for publication in the BMC Gastroenterology journal. However, before being published, the
following issues should be clarified to better convince readers, including:
1. The author should re-read the manuscript to correct the errors, for example, AUC value 536
on Page 10, line 222?
2. The author should revise Figure 1 to make the article more logical and understandable to
readers. For example, is the value 159 mentioned in Full-text?
3. In the supplementary section, the author should provide clinical information of 185 cases as
well as information on 171 samples used in this analysis.
4. Please consider using the term "correlation" instead of the expression "relationship/relation."
5. In Figure 7 about the nomogram, please explain more about the parameters. Commonly,
a nomogram should be used to quickly identify conditions (e.g., disease). Could the authors
explain how a clinician can quickly read the results from clinical tests using these six (06)
lncRNAs?
6. The study would be perfect and suitable for publication in the journal of BMC
Gastroenterology if at least one of these three (03) lncRNAs (Z94721.2, AL035461.2, and
AC051619.4) were verified experimentally. The author may consider performing a quick
quantitative qPCR analysis to verify the factual accuracy of this newly built model.
7. Finally, I strongly recommend that authors review the entire manuscript to revise the typos
and other related issues.
Please review this paper.
user
Title: Construction of a novel necroptosis-related lncRNA signature for prognosis prediction
in esophageal cancer
Version: 1 Date: 29 May 2022
Reviewer's report:
The research conducted by Yang Liu and colleagues under the title "Construction of a
novel necroptosis-related lncRNA signature for prognosis in esophageal cancer" shows the
significance in the scientific sense and future potential applications. The authors performed
the analysis by using a dataset of 171 samples obtained from the TCGA database with 160
esophageal cancer samples and 11 samples of normal esophageal tissue to seek necroptosis-
related lncRNAs as well as related genes in EC. Through multi-analysis using packages from
R software and others, the authors succeeded in building a predictive or prognostic model for
EC patients based on a group of biomarkers, including six necroptosis-associated lncRNAs.
The manuscript was also well prepared; therefore, I believe this study should be considered
for publication in the BMC Gastroenterology journal. However, before being published, the
following issues should be clarified to better convince readers, including:
1. The author should re-read the manuscript to correct the errors, for example, AUC value 536
on Page 10, line 222?
2. The author should revise Figure 1 to make the article more logical and understandable to
readers. For example, is the value 159 mentioned in Full-text?
3. In the supplementary section, the author should provide clinical information of 185 cases as
well as information on 171 samples used in this analysis.
4. Please consider using the term "correlation" instead of the expression "relationship/relation."
5. In Figure 7 about the nomogram, please explain more about the parameters. Commonly,
a nomogram should be used to quickly identify conditions (e.g., disease). Could the authors
explain how a clinician can quickly read the results from clinical tests using these six (06)
lncRNAs?
6. The study would be perfect and suitable for publication in the journal of BMC
Gastroenterology if at least one of these three (03) lncRNAs (Z94721.2, AL035461.2, and
AC051619.4) were verified experimentally. The author may consider performing a quick
quantitative qPCR analysis to verify the factual accuracy of this newly built model.
7. Finally, I strongly recommend that authors review the entire manuscript to revise the typos
and other related issues.
assistant