Reviewer's report
Title:Hyperammonia Induces Specific Liver Injury through an Intrinsic
Ca2+-independent Apoptosis Pathway
Version:2Date:2 May 2014
Reviewer: Manlio Vinciguerra
Reviewer's report:
This study is intended to study hyperammonia-specific liver injury. The study is of
relevance to the field as the effects of ammonia on the hepatocytes themselves
is not completely clear. The authors use established HepG2 cell models to show
that hyperammonia induces specific liver injury through an intrinsic
Ca2+independent apoptosis pathway. The design is clear and the methods are
sounds. The conclusions are consistent with the results. I have only minor but
essential comments.
Minor Essential Revisions
The manuscript needs extensive proof-reading and correction by an English
native.
Typos: Line 44, 51, 54, 125, 163, 176, 193, 194, 206, 215, 230, 244, 256, 257,
266
Insert spaces between one paragraph of Results and the other. Sometime the
spaces are there, sometime they are not there. Be consistent.
Line 149: “Add…”. This is not a lab protocol, but the Material&Methods section.
Be consistent between “h” and “hours” throughout the manuscript.
Lines 192-195, 230 are grammatically obscure.
Line 205: a sentence can’t start with “And”.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Hyperammonia Induces Specific Liver Injury through an Intrinsic
Ca2+-independent Apoptosis Pathway
Version:2Date:2 May 2014
Reviewer: Manlio Vinciguerra
Reviewer's report:
This study is intended to study hyperammonia-specific liver injury. The study is of
relevance to the field as the effects of ammonia on the hepatocytes themselves
is not completely clear. The authors use established HepG2 cell models to show
that hyperammonia induces specific liver injury through an intrinsic
Ca2+independent apoptosis pathway. The design is clear and the methods are
sounds. The conclusions are consistent with the results. I have only minor but
essential comments.
Minor Essential Revisions
The manuscript needs extensive proof-reading and correction by an English
native.
Typos: Line 44, 51, 54, 125, 163, 176, 193, 194, 206, 215, 230, 244, 256, 257,
266
Insert spaces between one paragraph of Results and the other. Sometime the
spaces are there, sometime they are not there. Be consistent.
Line 149: “Add…”. This is not a lab protocol, but the Material&Methods section.
Be consistent between “h” and “hours” throughout the manuscript.
Lines 192-195, 230 are grammatically obscure.
Line 205: a sentence can’t start with “And”.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Hyperammonia Induces Specific Liver Injury through an Intrinsic
Ca2+-independent Apoptosis Pathway
Version:2Date:2 May 2014
Reviewer: Manlio Vinciguerra
Reviewer's report:
This study is intended to study hyperammonia-specific liver injury. The study is of
relevance to the field as the effects of ammonia on the hepatocytes themselves
is not completely clear. The authors use established HepG2 cell models to show
that hyperammonia induces specific liver injury through an intrinsic
Ca2+independent apoptosis pathway. The design is clear and the methods are
sounds. The conclusions are consistent with the results. I have only minor but
essential comments.
Minor Essential Revisions
The manuscript needs extensive proof-reading and correction by an English
native.
Typos: Line 44, 51, 54, 125, 163, 176, 193, 194, 206, 215, 230, 244, 256, 257,
266
Insert spaces between one paragraph of Results and the other. Sometime the
spaces are there, sometime they are not there. Be consistent.
Line 149: “Add…”. This is not a lab protocol, but the Material&Methods section.
Be consistent between “h” and “hours” throughout the manuscript.
Lines 192-195, 230 are grammatically obscure.
Line 205: a sentence can’t start with “And”.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests
assistant