Title: Effect of disease duration on fecal biomarkers in ulcerative colitis: A prospective cohort
study
Version: 1 Date: 02 Aug 2022
Reviewer:  Sakuma Takahashi
Reviewer's report:
It is my pleasure to review the manuscript entitled "Effect of disease duration on fecal biomarkers
in ulcerative colitis: A prospective cohort study".
The manuscript has been revised well. However, I have several concerns that need to be
addressed before considering publication.
1) The authors write that "To avoid the effects of colonoscopic preparation or examination on
biomarkers, fecal samples for FC and FIT were collected the day before or after the
colonoscopy." on line 140-141, page 9.
I wonder FC and FIT collected the day after the colonoscopy are influenced colonoscopic
preparation or examination, especially biopsy procedures. We published some papers on fecal
biomaker, but we always collect fecal samples the day before the colonoscopy.
Please analyze whether the timing of fecal sampling influence the result or not.
2) The authors write that "We suspect that DNA damage via this mechanism may also alter the
inflammatory cytokine profile, like the apoptosis signal pathway due to DNA damage, in the
intestinal tract and may affect FC, which reflects neutrophils in the intestinal tract [39]." on line
330-334, page 20. I read the 39th paper of the references, but I could not find such result. I think
[39] might be wrong. 40th paper of the references might be correct.
3) The authors write that "A previous study evaluated the relationship between FC and disease
duration for CD by dividing patients into groups with disease duration less than 10 years and
ten years or longer. The authors reported that disease duration did not affect the diagnostic
usefulness of FC [40]." on line 342-345, page 20-21. I read the 40th paper of the references,
but I could not find such result. I think [40] might be wrong. 39th paper of the references might
be correct.
Title: Effect of disease duration on fecal biomarkers in ulcerative colitis: A prospective cohort
study
Version: 1 Date: 02 Aug 2022
Reviewer:  Sakuma Takahashi
Reviewer's report:
It is my pleasure to review the manuscript entitled "Effect of disease duration on fecal biomarkers
in ulcerative colitis: A prospective cohort study".
The manuscript has been revised well. However, I have several concerns that need to be
addressed before considering publication.
1) The authors write that "To avoid the effects of colonoscopic preparation or examination on
biomarkers, fecal samples for FC and FIT were collected the day before or after the
colonoscopy." on line 140-141, page 9.
I wonder FC and FIT collected the day after the colonoscopy are influenced colonoscopic
preparation or examination, especially biopsy procedures. We published some papers on fecal
biomaker, but we always collect fecal samples the day before the colonoscopy.
Please analyze whether the timing of fecal sampling influence the result or not.
2) The authors write that "We suspect that DNA damage via this mechanism may also alter the
inflammatory cytokine profile, like the apoptosis signal pathway due to DNA damage, in the
intestinal tract and may affect FC, which reflects neutrophils in the intestinal tract [39]." on line
330-334, page 20. I read the 39th paper of the references, but I could not find such result. I think
[39] might be wrong. 40th paper of the references might be correct.
3) The authors write that "A previous study evaluated the relationship between FC and disease
duration for CD by dividing patients into groups with disease duration less than 10 years and
ten years or longer. The authors reported that disease duration did not affect the diagnostic
usefulness of FC [40]." on line 342-345, page 20-21. I read the 40th paper of the references,
but I could not find such result. I think [40] might be wrong. 39th paper of the references might
be correct.
Please review this paper.
user
Title: Effect of disease duration on fecal biomarkers in ulcerative colitis: A prospective cohort
study
Version: 1 Date: 02 Aug 2022
Reviewer:  Sakuma Takahashi
Reviewer's report:
It is my pleasure to review the manuscript entitled "Effect of disease duration on fecal biomarkers
in ulcerative colitis: A prospective cohort study".
The manuscript has been revised well. However, I have several concerns that need to be
addressed before considering publication.
1) The authors write that "To avoid the effects of colonoscopic preparation or examination on
biomarkers, fecal samples for FC and FIT were collected the day before or after the
colonoscopy." on line 140-141, page 9.
I wonder FC and FIT collected the day after the colonoscopy are influenced colonoscopic
preparation or examination, especially biopsy procedures. We published some papers on fecal
biomaker, but we always collect fecal samples the day before the colonoscopy.
Please analyze whether the timing of fecal sampling influence the result or not.
2) The authors write that "We suspect that DNA damage via this mechanism may also alter the
inflammatory cytokine profile, like the apoptosis signal pathway due to DNA damage, in the
intestinal tract and may affect FC, which reflects neutrophils in the intestinal tract [39]." on line
330-334, page 20. I read the 39th paper of the references, but I could not find such result. I think
[39] might be wrong. 40th paper of the references might be correct.
3) The authors write that "A previous study evaluated the relationship between FC and disease
duration for CD by dividing patients into groups with disease duration less than 10 years and
ten years or longer. The authors reported that disease duration did not affect the diagnostic
usefulness of FC [40]." on line 342-345, page 20-21. I read the 40th paper of the references,
but I could not find such result. I think [40] might be wrong. 39th paper of the references might
be correct.
assistant