Title: Overexpression of Protein Kinase C iota as a Prognostic Marker and Its Role in Oxaliplatin
Resistance in colorectal Cancer
Version: 4 Date: 18 Jun 2025
Reviewer's report:
To enhance the scientific rigor and contextual relevance of the manuscript, the authors are
encouraged to incorporate several recent studies related to colorectal cancer prognosis and
chemotherapy resistance. BEAN1 has been shown to correlate with poor prognosis and
oxaliplatin resistance in rectal adenocarcinoma (PMID: 39276259), supporting the discussion
of PKC#’s role in chemoresistance. Immune-related prognostic markers such as pan-immune-
inflammatory values (PMID: 40046636) and combined NP/LHb indices with monocyte
count (PMID: 40087531) offer additional evidence for the clinical significance of systemic
inflammation in colorectal cancer, which aligns with the manuscript’s findings. Furthermore,
metabolic indicators including serum calcium (PMID: 40162418) have demonstrated prognostic
value and can complement the discussion of body weight and lipid profiles in predicting
progression-free survival. Finally, research on nutritional and inflammatory markers for
anastomotic leakage (PMID: 40291866) can be cited in the Introduction to highlight the
complexity of CRC management and the importance of comprehensive prognostic evaluation.
Incorporating these references will improve the manuscript’s depth, strengthen its discussion,
and situate the findings within the broader landscape of colorectal cancer research.
Title: Overexpression of Protein Kinase C iota as a Prognostic Marker and Its Role in Oxaliplatin
Resistance in colorectal Cancer
Version: 4 Date: 18 Jun 2025
Reviewer's report:
To enhance the scientific rigor and contextual relevance of the manuscript, the authors are
encouraged to incorporate several recent studies related to colorectal cancer prognosis and
chemotherapy resistance. BEAN1 has been shown to correlate with poor prognosis and
oxaliplatin resistance in rectal adenocarcinoma (PMID: 39276259), supporting the discussion
of PKC#’s role in chemoresistance. Immune-related prognostic markers such as pan-immune-
inflammatory values (PMID: 40046636) and combined NP/LHb indices with monocyte
count (PMID: 40087531) offer additional evidence for the clinical significance of systemic
inflammation in colorectal cancer, which aligns with the manuscript’s findings. Furthermore,
metabolic indicators including serum calcium (PMID: 40162418) have demonstrated prognostic
value and can complement the discussion of body weight and lipid profiles in predicting
progression-free survival. Finally, research on nutritional and inflammatory markers for
anastomotic leakage (PMID: 40291866) can be cited in the Introduction to highlight the
complexity of CRC management and the importance of comprehensive prognostic evaluation.
Incorporating these references will improve the manuscript’s depth, strengthen its discussion,
and situate the findings within the broader landscape of colorectal cancer research.
Please review this paper.
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Title: Overexpression of Protein Kinase C iota as a Prognostic Marker and Its Role in Oxaliplatin
Resistance in colorectal Cancer
Version: 4 Date: 18 Jun 2025
Reviewer's report:
To enhance the scientific rigor and contextual relevance of the manuscript, the authors are
encouraged to incorporate several recent studies related to colorectal cancer prognosis and
chemotherapy resistance. BEAN1 has been shown to correlate with poor prognosis and
oxaliplatin resistance in rectal adenocarcinoma (PMID: 39276259), supporting the discussion
of PKC#’s role in chemoresistance. Immune-related prognostic markers such as pan-immune-
inflammatory values (PMID: 40046636) and combined NP/LHb indices with monocyte
count (PMID: 40087531) offer additional evidence for the clinical significance of systemic
inflammation in colorectal cancer, which aligns with the manuscript’s findings. Furthermore,
metabolic indicators including serum calcium (PMID: 40162418) have demonstrated prognostic
value and can complement the discussion of body weight and lipid profiles in predicting
progression-free survival. Finally, research on nutritional and inflammatory markers for
anastomotic leakage (PMID: 40291866) can be cited in the Introduction to highlight the
complexity of CRC management and the importance of comprehensive prognostic evaluation.
Incorporating these references will improve the manuscript’s depth, strengthen its discussion,
and situate the findings within the broader landscape of colorectal cancer research.
assistant