Reviewer's report
Title:MYC, FBXW7 and TP53 copy number variation and expression in Gastric
Cancer
Version: 2Date:21 March 2013
Reviewer: Yueyong Liu
Reviewer's report:
This manuscript is a revised version of previous submission. The authors
improved the results, discussion and added zymography data to this manuscript.
Due to the complexity of oncogene and tumor suppressor in the tumorigenesis,
it’s hard to straighten out the relationship of MYC, FBXW7 and TP53 under
several parameters including copy number, mRNA level and protein expression
level. Even so this manuscript still brings to us lots of interesting information
including the firstly reported deregulation of MYC (amplification) and FBXW7
(LOH) associated with lymph node metastasis and stage III-IV disease. The
authors made great efforts in investigating gene status of MYC, FBXW7 and
TP53 in three different levels in GC tumors and cancer cell lines which is
potentially useful for the researchers in this related field. Based on the data
presented, it is worthy of being published in BMC gastroenterology journal.
Minor concerns:
1. The authors added and showed the increased active MMP9 form in ACP02
cell line which is more invasive as compared to ACP02. Increased MMP9 and
reduced FBXW7 level can promote cell motility. However, ACP2 showed
impaired migration capability instead, please specify.
2. Typos: a. line 2 in results: deletion instead of delection.
b. line 2 in subtitle 2 of results: non-neoplastic instead of non-tissue
c.line 10 in subtitle 4 of results: punctiform instead of puntiform
d. the symbol in table2: age 50.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Reviewer's report
Title:MYC, FBXW7 and TP53 copy number variation and expression in Gastric
Cancer
Version: 2Date:21 March 2013
Reviewer: Yueyong Liu
Reviewer's report:
This manuscript is a revised version of previous submission. The authors
improved the results, discussion and added zymography data to this manuscript.
Due to the complexity of oncogene and tumor suppressor in the tumorigenesis,
it’s hard to straighten out the relationship of MYC, FBXW7 and TP53 under
several parameters including copy number, mRNA level and protein expression
level. Even so this manuscript still brings to us lots of interesting information
including the firstly reported deregulation of MYC (amplification) and FBXW7
(LOH) associated with lymph node metastasis and stage III-IV disease. The
authors made great efforts in investigating gene status of MYC, FBXW7 and
TP53 in three different levels in GC tumors and cancer cell lines which is
potentially useful for the researchers in this related field. Based on the data
presented, it is worthy of being published in BMC gastroenterology journal.
Minor concerns:
1. The authors added and showed the increased active MMP9 form in ACP02
cell line which is more invasive as compared to ACP02. Increased MMP9 and
reduced FBXW7 level can promote cell motility. However, ACP2 showed
impaired migration capability instead, please specify.
2. Typos: a. line 2 in results: deletion instead of delection.
b. line 2 in subtitle 2 of results: non-neoplastic instead of non-tissue
c.line 10 in subtitle 4 of results: punctiform instead of puntiform
d. the symbol in table2: age 50.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Please review this paper.
user
Reviewer's report
Title:MYC, FBXW7 and TP53 copy number variation and expression in Gastric
Cancer
Version: 2Date:21 March 2013
Reviewer: Yueyong Liu
Reviewer's report:
This manuscript is a revised version of previous submission. The authors
improved the results, discussion and added zymography data to this manuscript.
Due to the complexity of oncogene and tumor suppressor in the tumorigenesis,
it’s hard to straighten out the relationship of MYC, FBXW7 and TP53 under
several parameters including copy number, mRNA level and protein expression
level. Even so this manuscript still brings to us lots of interesting information
including the firstly reported deregulation of MYC (amplification) and FBXW7
(LOH) associated with lymph node metastasis and stage III-IV disease. The
authors made great efforts in investigating gene status of MYC, FBXW7 and
TP53 in three different levels in GC tumors and cancer cell lines which is
potentially useful for the researchers in this related field. Based on the data
presented, it is worthy of being published in BMC gastroenterology journal.
Minor concerns:
1. The authors added and showed the increased active MMP9 form in ACP02
cell line which is more invasive as compared to ACP02. Increased MMP9 and
reduced FBXW7 level can promote cell motility. However, ACP2 showed
impaired migration capability instead, please specify.
2. Typos: a. line 2 in results: deletion instead of delection.
b. line 2 in subtitle 2 of results: non-neoplastic instead of non-tissue
c.line 10 in subtitle 4 of results: punctiform instead of puntiform
d. the symbol in table2: age 50.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
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