Reviewer's report
Title:Pharmacologic cholinesterase inhibition improves survival in
acetaminophen-induced acute liver failure in the mouse
Version:1Date:11 February 2014
Reviewer: Mitchell Fink
Reviewer's report:
Major Comments:
1. Although neostigmine pre-treatment prolonged survival, administration of this
drug failed to change outcome. 100% of the animals died irrespective of whether
or not they received neostigmine. The authors need to address this issue. Was
their model too overwhelming? If so, additional studies using a model with lower
mortality would be helpful. Was the dose of neostigmine inadequate? If so,
additional, dose-response studies would strengthen the paper.
2. N-acetylcysteine (NAC) is the current "gold standard" approach for managing
acetominophen overdoses in patients. Thus, it is appropriate to ask whether
survival can be improved adding neostigmine to a treatment regimen based on
administration of NAC.
Minor Comment:
1. Neostigmine is a (reversible) acetylcholinesterase (not acetylcholine) inhibitor.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
Reviewer's report
Title:Pharmacologic cholinesterase inhibition improves survival in
acetaminophen-induced acute liver failure in the mouse
Version:1Date:11 February 2014
Reviewer: Mitchell Fink
Reviewer's report:
Major Comments:
1. Although neostigmine pre-treatment prolonged survival, administration of this
drug failed to change outcome. 100% of the animals died irrespective of whether
or not they received neostigmine. The authors need to address this issue. Was
their model too overwhelming? If so, additional studies using a model with lower
mortality would be helpful. Was the dose of neostigmine inadequate? If so,
additional, dose-response studies would strengthen the paper.
2. N-acetylcysteine (NAC) is the current "gold standard" approach for managing
acetominophen overdoses in patients. Thus, it is appropriate to ask whether
survival can be improved adding neostigmine to a treatment regimen based on
administration of NAC.
Minor Comment:
1. Neostigmine is a (reversible) acetylcholinesterase (not acetylcholine) inhibitor.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
Please review this paper.
user
Reviewer's report
Title:Pharmacologic cholinesterase inhibition improves survival in
acetaminophen-induced acute liver failure in the mouse
Version:1Date:11 February 2014
Reviewer: Mitchell Fink
Reviewer's report:
Major Comments:
1. Although neostigmine pre-treatment prolonged survival, administration of this
drug failed to change outcome. 100% of the animals died irrespective of whether
or not they received neostigmine. The authors need to address this issue. Was
their model too overwhelming? If so, additional studies using a model with lower
mortality would be helpful. Was the dose of neostigmine inadequate? If so,
additional, dose-response studies would strengthen the paper.
2. N-acetylcysteine (NAC) is the current "gold standard" approach for managing
acetominophen overdoses in patients. Thus, it is appropriate to ask whether
survival can be improved adding neostigmine to a treatment regimen based on
administration of NAC.
Minor Comment:
1. Neostigmine is a (reversible) acetylcholinesterase (not acetylcholine) inhibitor.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
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