Reviewer's report
Title:TNF-alpha-induced up-regulation of pro-inflammatory cytokines is delayed
by phosphatidylcholine in intestinal epithelial cells
Version: 3Date:24 October 2008
Reviewer: Jan Wehkamp
Reviewer's report:
The data by Treede at al. are sound, fit well to already published data and the
experiments seem well done. I only have some minor comments which might add
the quality of paper discusssion.
Figures: in my view, the figures would benefit from the inclusion of some
statistical analysis- even though the differences seem very clear.
Introduction: It is possible - or even likely- that the immune response is
adaequate and secondary due to the entry of bacteria. The authors should make
their first sentnce statement more carefull in less exclusive.
Discussion and introduction: NFkB also regulates many protective factors
including inducible beta defensins (e.g. induction of beta defensins by probiotic
bacteria, Infection and Immunity 2004). Eptithelial knock out of NFkB leads to
intestinal inflammation at least partyl associated with a lack of beta defensins.
Both aspects should be added in order to avoid a too simplistic view of nFkB and
its role in inflammatory regulation.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Reviewer's report
Title:TNF-alpha-induced up-regulation of pro-inflammatory cytokines is delayed
by phosphatidylcholine in intestinal epithelial cells
Version: 3Date:24 October 2008
Reviewer: Jan Wehkamp
Reviewer's report:
The data by Treede at al. are sound, fit well to already published data and the
experiments seem well done. I only have some minor comments which might add
the quality of paper discusssion.
Figures: in my view, the figures would benefit from the inclusion of some
statistical analysis- even though the differences seem very clear.
Introduction: It is possible - or even likely- that the immune response is
adaequate and secondary due to the entry of bacteria. The authors should make
their first sentnce statement more carefull in less exclusive.
Discussion and introduction: NFkB also regulates many protective factors
including inducible beta defensins (e.g. induction of beta defensins by probiotic
bacteria, Infection and Immunity 2004). Eptithelial knock out of NFkB leads to
intestinal inflammation at least partyl associated with a lack of beta defensins.
Both aspects should be added in order to avoid a too simplistic view of nFkB and
its role in inflammatory regulation.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Please review this paper.
user
Reviewer's report
Title:TNF-alpha-induced up-regulation of pro-inflammatory cytokines is delayed
by phosphatidylcholine in intestinal epithelial cells
Version: 3Date:24 October 2008
Reviewer: Jan Wehkamp
Reviewer's report:
The data by Treede at al. are sound, fit well to already published data and the
experiments seem well done. I only have some minor comments which might add
the quality of paper discusssion.
Figures: in my view, the figures would benefit from the inclusion of some
statistical analysis- even though the differences seem very clear.
Introduction: It is possible - or even likely- that the immune response is
adaequate and secondary due to the entry of bacteria. The authors should make
their first sentnce statement more carefull in less exclusive.
Discussion and introduction: NFkB also regulates many protective factors
including inducible beta defensins (e.g. induction of beta defensins by probiotic
bacteria, Infection and Immunity 2004). Eptithelial knock out of NFkB leads to
intestinal inflammation at least partyl associated with a lack of beta defensins.
Both aspects should be added in order to avoid a too simplistic view of nFkB and
its role in inflammatory regulation.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
assistant