Reviewer's report
Title:Ringer's Lactate improves liver recovery in a murine model of
acetaminophen toxicity
Version: 3Date:29 September 2011
Reviewer: Jim P. Luyendyk
Reviewer's report:
The 48 hour clinical chemistry data are sufficiently critical as to deserve inclusion
in the results section at a minimum, but should be included in the figure package.
It seems likely that issues with figure panel arrangement can be overcome to
accomodate this change. It is particularly important to include these data insofar
as the authors indicate with respect to PCNA data that necrosis is reduced from
25% to 2% by RLS treatment, despite a lack of significant change in ALT at
either 24 or 48 hours.
It is surprising that PCNA-positive hepatocytes were not present in livers of
APAP-treated mice and that the vast majority of PCNA positive cells were
inflammatory cells. Previous studies have definitively shown that hepatocytes
bordering areas of necrosis express PCNA during injury progression.
Clarificiation is required.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Ringer's Lactate improves liver recovery in a murine model of
acetaminophen toxicity
Version: 3Date:29 September 2011
Reviewer: Jim P. Luyendyk
Reviewer's report:
The 48 hour clinical chemistry data are sufficiently critical as to deserve inclusion
in the results section at a minimum, but should be included in the figure package.
It seems likely that issues with figure panel arrangement can be overcome to
accomodate this change. It is particularly important to include these data insofar
as the authors indicate with respect to PCNA data that necrosis is reduced from
25% to 2% by RLS treatment, despite a lack of significant change in ALT at
either 24 or 48 hours.
It is surprising that PCNA-positive hepatocytes were not present in livers of
APAP-treated mice and that the vast majority of PCNA positive cells were
inflammatory cells. Previous studies have definitively shown that hepatocytes
bordering areas of necrosis express PCNA during injury progression.
Clarificiation is required.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Ringer's Lactate improves liver recovery in a murine model of
acetaminophen toxicity
Version: 3Date:29 September 2011
Reviewer: Jim P. Luyendyk
Reviewer's report:
The 48 hour clinical chemistry data are sufficiently critical as to deserve inclusion
in the results section at a minimum, but should be included in the figure package.
It seems likely that issues with figure panel arrangement can be overcome to
accomodate this change. It is particularly important to include these data insofar
as the authors indicate with respect to PCNA data that necrosis is reduced from
25% to 2% by RLS treatment, despite a lack of significant change in ALT at
either 24 or 48 hours.
It is surprising that PCNA-positive hepatocytes were not present in livers of
APAP-treated mice and that the vast majority of PCNA positive cells were
inflammatory cells. Previous studies have definitively shown that hepatocytes
bordering areas of necrosis express PCNA during injury progression.
Clarificiation is required.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
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