Title: Potential association of eEF1A dimethylation at lysine 55 in the basal area of Helicobacter
pylori-eradicated gastric mucosa with the risk of gastric cancer: A retrospective observational
study
Version: 1 Date: 11 Jul 2022
Reviewer's report:
1. I have major concerns regarding the English language used in this manuscript. The English
language needs improvement and there are some errors in the manuscript.
2. The Objective section of the abstract should specify that the efficacyness of H. pylori
eradication therapy was effect on eEF1A dimethylation and on gastric cancer risk.
3. The scientific hypothesis should be defined according to all aims that has been tested in this
study.
In the Introduction section:
4. There were not references addressed the importance factors such as H.pylori strain or virulence
factor in creation cancer.
5. you should explain what have eradication rate shown disappointing success in Japan.
In the Materials and Methods section:
6. According to patients enrolled between 2001 and 2018 with a total of 115 gastric biopsies
obtained, exclusion criteria are poorly defined in the Materials and Methods section.
7. It is not explained why they decided area of detection in surface, middle and basal. Which
was the outcome or H. pylori colonization model?
8. The description of histological analysis should go together with the sampling of gastric
biopsies or merged with “Study Subjects and sampling“.
9. Please provide detailed of Follow-up statement.
10. The definition of H. pylori eradication should be provided: negative stool antigen test plus
negative rapid urease test plus negative histology? In addition, the time when the eradication
was confirmed should be provided.
11. Although it is not the main goal of this study, it might be interesting to provide a figure with
representative cell transfection results (in Method section or as supplementary file).
In the Results section:
12. I recommend to delete references cited in Results section. It should be mentioned in
Introduction section.
13. Have Nanong and Oct4 been quantified in IM and GC that show the high expression level
lead to the association of carcinogenesis?  If so, please include those figure.
14. Was "normal" expression restored of control METTL13-depleted TMK1 cells? please
include those data. This provide a helpful point of control the experiment.
In the Discussion section:
15. The authors point out that “there was no significant increase in eEF1A dimethylation in the
IM area in eradicated patients with GC incidence”. It should be explained some discussion.
16. It should be clearer possible of H. pylori infection which generally occur in the first year
after eradication, or # 1 year after eradication.
17. The list of the references should be updated according to the recent research in this field.
18. The importance of overexpression of many factors associated gastric cancer should be
discussed.
19. Compare the way you work with other methods for examples.
20. This article shows the importance to field that indicates the H. pylori infection and
related disease. Therefore, discussion about H. pylori virulence factor or residue molecules -
related disease should added to be risk of GC. 21. I recommend please rewrite the discussion
extensively.
22. Acknowledgements should be made only to those who have made a substantial contribution
to the study.
Title: Potential association of eEF1A dimethylation at lysine 55 in the basal area of Helicobacter
pylori-eradicated gastric mucosa with the risk of gastric cancer: A retrospective observational
study
Version: 1 Date: 11 Jul 2022
Reviewer's report:
1. I have major concerns regarding the English language used in this manuscript. The English
language needs improvement and there are some errors in the manuscript.
2. The Objective section of the abstract should specify that the efficacyness of H. pylori
eradication therapy was effect on eEF1A dimethylation and on gastric cancer risk.
3. The scientific hypothesis should be defined according to all aims that has been tested in this
study.
In the Introduction section:
4. There were not references addressed the importance factors such as H.pylori strain or virulence
factor in creation cancer.
5. you should explain what have eradication rate shown disappointing success in Japan.
In the Materials and Methods section:
6. According to patients enrolled between 2001 and 2018 with a total of 115 gastric biopsies
obtained, exclusion criteria are poorly defined in the Materials and Methods section.
7. It is not explained why they decided area of detection in surface, middle and basal. Which
was the outcome or H. pylori colonization model?
8. The description of histological analysis should go together with the sampling of gastric
biopsies or merged with “Study Subjects and sampling“.
9. Please provide detailed of Follow-up statement.
10. The definition of H. pylori eradication should be provided: negative stool antigen test plus
negative rapid urease test plus negative histology? In addition, the time when the eradication
was confirmed should be provided.
11. Although it is not the main goal of this study, it might be interesting to provide a figure with
representative cell transfection results (in Method section or as supplementary file).
In the Results section:
12. I recommend to delete references cited in Results section. It should be mentioned in
Introduction section.
13. Have Nanong and Oct4 been quantified in IM and GC that show the high expression level
lead to the association of carcinogenesis?  If so, please include those figure.
14. Was "normal" expression restored of control METTL13-depleted TMK1 cells? please
include those data. This provide a helpful point of control the experiment.
In the Discussion section:
15. The authors point out that “there was no significant increase in eEF1A dimethylation in the
IM area in eradicated patients with GC incidence”. It should be explained some discussion.
16. It should be clearer possible of H. pylori infection which generally occur in the first year
after eradication, or # 1 year after eradication.
17. The list of the references should be updated according to the recent research in this field.
18. The importance of overexpression of many factors associated gastric cancer should be
discussed.
19. Compare the way you work with other methods for examples.
20. This article shows the importance to field that indicates the H. pylori infection and
related disease. Therefore, discussion about H. pylori virulence factor or residue molecules -
related disease should added to be risk of GC. 21. I recommend please rewrite the discussion
extensively.
22. Acknowledgements should be made only to those who have made a substantial contribution
to the study.
Please review this paper.
user
Title: Potential association of eEF1A dimethylation at lysine 55 in the basal area of Helicobacter
pylori-eradicated gastric mucosa with the risk of gastric cancer: A retrospective observational
study
Version: 1 Date: 11 Jul 2022
Reviewer's report:
1. I have major concerns regarding the English language used in this manuscript. The English
language needs improvement and there are some errors in the manuscript.
2. The Objective section of the abstract should specify that the efficacyness of H. pylori
eradication therapy was effect on eEF1A dimethylation and on gastric cancer risk.
3. The scientific hypothesis should be defined according to all aims that has been tested in this
study.
In the Introduction section:
4. There were not references addressed the importance factors such as H.pylori strain or virulence
factor in creation cancer.
5. you should explain what have eradication rate shown disappointing success in Japan.
In the Materials and Methods section:
6. According to patients enrolled between 2001 and 2018 with a total of 115 gastric biopsies
obtained, exclusion criteria are poorly defined in the Materials and Methods section.
7. It is not explained why they decided area of detection in surface, middle and basal. Which
was the outcome or H. pylori colonization model?
8. The description of histological analysis should go together with the sampling of gastric
biopsies or merged with “Study Subjects and sampling“.
9. Please provide detailed of Follow-up statement.
10. The definition of H. pylori eradication should be provided: negative stool antigen test plus
negative rapid urease test plus negative histology? In addition, the time when the eradication
was confirmed should be provided.
11. Although it is not the main goal of this study, it might be interesting to provide a figure with
representative cell transfection results (in Method section or as supplementary file).
In the Results section:
12. I recommend to delete references cited in Results section. It should be mentioned in
Introduction section.
13. Have Nanong and Oct4 been quantified in IM and GC that show the high expression level
lead to the association of carcinogenesis?  If so, please include those figure.
14. Was "normal" expression restored of control METTL13-depleted TMK1 cells? please
include those data. This provide a helpful point of control the experiment.
In the Discussion section:
15. The authors point out that “there was no significant increase in eEF1A dimethylation in the
IM area in eradicated patients with GC incidence”. It should be explained some discussion.
16. It should be clearer possible of H. pylori infection which generally occur in the first year
after eradication, or # 1 year after eradication.
17. The list of the references should be updated according to the recent research in this field.
18. The importance of overexpression of many factors associated gastric cancer should be
discussed.
19. Compare the way you work with other methods for examples.
20. This article shows the importance to field that indicates the H. pylori infection and
related disease. Therefore, discussion about H. pylori virulence factor or residue molecules -
related disease should added to be risk of GC. 21. I recommend please rewrite the discussion
extensively.
22. Acknowledgements should be made only to those who have made a substantial contribution
to the study.
assistant