Title: Markers of immune dysregulation in response to the ageing gut: insights from aged murine
gut microbiota transplants
Version: 1 Date: 29 Jul 2022
Reviewer's report:
The manuscript “Markers of immune dysregulation in response to the ageing gut: insights
from aged murine gut microbiota transplants” by Prokopidis et al. describes the analysis of
transcriptomic data of another published study by Kundu and colleagues from 2019 (DOI:
10.1126/scitranslmed.aau4760) that colonized young germ-free mice with microbiomes of
either old or young mice to identify immune-associated differentially expressed genes. The
entire manuscript is based on minor analyses (DEGs, GO enrichment, network) of published
transcriptional data. The authors did not perform any validation experiments. The findings do
not significantly extend current knowledge as an increased “inflammatory” response by an
aged microbiome was already known, e.g. DOI 10.1016/j.chom.2017.03.002. The association
analyses between DEG expression and abundance of immune infiltrates based on another
published dataset (TCGA and GTEx projects) is more original, yet there is no validation of any
of these potential associations and thus it remains speculative. Therefore, in its current form the
manuscript does not warrant publication.
Title: Markers of immune dysregulation in response to the ageing gut: insights from aged murine
gut microbiota transplants
Version: 1 Date: 29 Jul 2022
Reviewer's report:
The manuscript “Markers of immune dysregulation in response to the ageing gut: insights
from aged murine gut microbiota transplants” by Prokopidis et al. describes the analysis of
transcriptomic data of another published study by Kundu and colleagues from 2019 (DOI:
10.1126/scitranslmed.aau4760) that colonized young germ-free mice with microbiomes of
either old or young mice to identify immune-associated differentially expressed genes. The
entire manuscript is based on minor analyses (DEGs, GO enrichment, network) of published
transcriptional data. The authors did not perform any validation experiments. The findings do
not significantly extend current knowledge as an increased “inflammatory” response by an
aged microbiome was already known, e.g. DOI 10.1016/j.chom.2017.03.002. The association
analyses between DEG expression and abundance of immune infiltrates based on another
published dataset (TCGA and GTEx projects) is more original, yet there is no validation of any
of these potential associations and thus it remains speculative. Therefore, in its current form the
manuscript does not warrant publication.
Please review this paper.
user
Title: Markers of immune dysregulation in response to the ageing gut: insights from aged murine
gut microbiota transplants
Version: 1 Date: 29 Jul 2022
Reviewer's report:
The manuscript “Markers of immune dysregulation in response to the ageing gut: insights
from aged murine gut microbiota transplants” by Prokopidis et al. describes the analysis of
transcriptomic data of another published study by Kundu and colleagues from 2019 (DOI:
10.1126/scitranslmed.aau4760) that colonized young germ-free mice with microbiomes of
either old or young mice to identify immune-associated differentially expressed genes. The
entire manuscript is based on minor analyses (DEGs, GO enrichment, network) of published
transcriptional data. The authors did not perform any validation experiments. The findings do
not significantly extend current knowledge as an increased “inflammatory” response by an
aged microbiome was already known, e.g. DOI 10.1016/j.chom.2017.03.002. The association
analyses between DEG expression and abundance of immune infiltrates based on another
published dataset (TCGA and GTEx projects) is more original, yet there is no validation of any
of these potential associations and thus it remains speculative. Therefore, in its current form the
manuscript does not warrant publication.
assistant