Reviewer's report
Title:Transplantation of human fetal biliary tree stem/progenitor cells into two
patients with advanced liver cirrhosis
Version:1Date:3 September 2014
Reviewer: fusheng F.W. Wang
Reviewer's report:
This is a very interesting clinical trial. The author transfused human fetal biliary
tree stem/progenitor cells to treat advanced liver cirrhosis. The result showed
that the procedure was safe, and both patients got biochemical and clinical
improvement during the short time follow-up studies. Although there were only
two patients in their treatment, but it really provided some important suggestions
for the forthcoming application. One is the whole isolation procedure was carried
out under “European guidelines of GMP for medicinal products for human use”,
which will promote its commercialization. The other one is fetal biliary tree stem
cells are a suitable and large source for cell therapy, which will overcomes
limitations of hepatocyte-based therapy.
Major Compulsory Revisions:
1. We noticed that the highest purity for human fetal biliary tree stem/progenitor
cells was only 73.9%, if the rest of other cells will affect the treatment effeciency
? and how to improve the purity in the future.
Discretionary Revisions:
1.Both patients showed biochemical and clinical improvement during the 6 month
follow-up study, if it indicate that the second treatment should be give at this time
point(6 month)?
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Transplantation of human fetal biliary tree stem/progenitor cells into two
patients with advanced liver cirrhosis
Version:1Date:3 September 2014
Reviewer: fusheng F.W. Wang
Reviewer's report:
This is a very interesting clinical trial. The author transfused human fetal biliary
tree stem/progenitor cells to treat advanced liver cirrhosis. The result showed
that the procedure was safe, and both patients got biochemical and clinical
improvement during the short time follow-up studies. Although there were only
two patients in their treatment, but it really provided some important suggestions
for the forthcoming application. One is the whole isolation procedure was carried
out under “European guidelines of GMP for medicinal products for human use”,
which will promote its commercialization. The other one is fetal biliary tree stem
cells are a suitable and large source for cell therapy, which will overcomes
limitations of hepatocyte-based therapy.
Major Compulsory Revisions:
1. We noticed that the highest purity for human fetal biliary tree stem/progenitor
cells was only 73.9%, if the rest of other cells will affect the treatment effeciency
? and how to improve the purity in the future.
Discretionary Revisions:
1.Both patients showed biochemical and clinical improvement during the 6 month
follow-up study, if it indicate that the second treatment should be give at this time
point(6 month)?
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Transplantation of human fetal biliary tree stem/progenitor cells into two
patients with advanced liver cirrhosis
Version:1Date:3 September 2014
Reviewer: fusheng F.W. Wang
Reviewer's report:
This is a very interesting clinical trial. The author transfused human fetal biliary
tree stem/progenitor cells to treat advanced liver cirrhosis. The result showed
that the procedure was safe, and both patients got biochemical and clinical
improvement during the short time follow-up studies. Although there were only
two patients in their treatment, but it really provided some important suggestions
for the forthcoming application. One is the whole isolation procedure was carried
out under “European guidelines of GMP for medicinal products for human use”,
which will promote its commercialization. The other one is fetal biliary tree stem
cells are a suitable and large source for cell therapy, which will overcomes
limitations of hepatocyte-based therapy.
Major Compulsory Revisions:
1. We noticed that the highest purity for human fetal biliary tree stem/progenitor
cells was only 73.9%, if the rest of other cells will affect the treatment effeciency
? and how to improve the purity in the future.
Discretionary Revisions:
1.Both patients showed biochemical and clinical improvement during the 6 month
follow-up study, if it indicate that the second treatment should be give at this time
point(6 month)?
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I declare that I have no competing interests
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