Title: Efficacy of extracorporeal immunoadsorption therapy for removal of HBsAg
Version: 4 Date: 03 May 2025
Reviewer's report:
Dear Sirs,
Good work on changes from the past versions.  But still some points which confuse me:
1. p. 2.3 is more about agarose matrix choice. not about Adsorption efficacy. In materials better
to show HOW you do experiments.
2. 2.4. i s about ligand concentration determination.
3. Dynamic and static adsorption in vitro more show to resulted immunosorbent capacity.  This
parameter best describes your immunosorbent properties.
4. If you are claiming what your sorbent can remove HBV DNA you shall discuss it.
Theoretically it is impossible. It may be a result of the action of rabbit’s immune system.
5. I miss the explanation between in vitro and in vivo efficacy differences.
Probably it is my subjective impression, but some information presented in “Materials and
Methods” shall be moved to “Discussion” and results, and some information from “Discussion”
shall be described in “Materials and Methods”.  I also placed some comments in the file attached.
Kind regards,
Title: Efficacy of extracorporeal immunoadsorption therapy for removal of HBsAg
Version: 4 Date: 03 May 2025
Reviewer's report:
Dear Sirs,
Good work on changes from the past versions.  But still some points which confuse me:
1. p. 2.3 is more about agarose matrix choice. not about Adsorption efficacy. In materials better
to show HOW you do experiments.
2. 2.4. i s about ligand concentration determination.
3. Dynamic and static adsorption in vitro more show to resulted immunosorbent capacity.  This
parameter best describes your immunosorbent properties.
4. If you are claiming what your sorbent can remove HBV DNA you shall discuss it.
Theoretically it is impossible. It may be a result of the action of rabbit’s immune system.
5. I miss the explanation between in vitro and in vivo efficacy differences.
Probably it is my subjective impression, but some information presented in “Materials and
Methods” shall be moved to “Discussion” and results, and some information from “Discussion”
shall be described in “Materials and Methods”.  I also placed some comments in the file attached.
Kind regards,
Please review this paper.
user
Title: Efficacy of extracorporeal immunoadsorption therapy for removal of HBsAg
Version: 4 Date: 03 May 2025
Reviewer's report:
Dear Sirs,
Good work on changes from the past versions.  But still some points which confuse me:
1. p. 2.3 is more about agarose matrix choice. not about Adsorption efficacy. In materials better
to show HOW you do experiments.
2. 2.4. i s about ligand concentration determination.
3. Dynamic and static adsorption in vitro more show to resulted immunosorbent capacity.  This
parameter best describes your immunosorbent properties.
4. If you are claiming what your sorbent can remove HBV DNA you shall discuss it.
Theoretically it is impossible. It may be a result of the action of rabbit’s immune system.
5. I miss the explanation between in vitro and in vivo efficacy differences.
Probably it is my subjective impression, but some information presented in “Materials and
Methods” shall be moved to “Discussion” and results, and some information from “Discussion”
shall be described in “Materials and Methods”.  I also placed some comments in the file attached.
Kind regards,
assistant