Title: Associations between changes in the gut microbiota and liver cirrhosis: a systematic
review and meta-analysis
Version: 0 Date: 11 Oct 2024
Reviewer's report:
This is an interesting systematic review, worthy of publication after correction
1) the abstract should not describe in detail the biodiversity indices, especially with numerical
characteristics - this is not interesting;
2) the abstract should indicate as many taxa as possible that showed significant and insignificant
differences with the control groups, especially at the level of higher taxa;
3) the conclusion indicates minor taxa that have a minimal effect on the macroorganism and are
unlikely to be useful in the  analysis of cirrhosis; authors have  good data on the differential
content of main taxa were and should indicate them  in the conclusions (without numerical
characteristics);
4) intestinal microbiota cannot be used to differentiate patients with cirrhosis from healthy
individuals - there are simpler methods for this; it could be useful in differentiating compensated
cirrhosis from chronic liver diseases at the pre-cirrhotic stage, but this was not studied in your
work, so this statement should be excluded;
5) a number of authors (https://pubmed.ncbi.nlm.nih.gov/34131470/; https://
pubmed.ncbi.nlm.nih.gov/24374295/) proposed dysbiosis ratios for the analysis of gut
microbiota in cirrhosis; this should be discussed based on your data;
6) it should be noted that in cirrhosis, the abundance of taxa of harmful facultative anaerobes
(Bacilli; Proteobacteria; families of these taxa) that are able to survive in oxygenated tissues
and are therefore capable of bacterial translocation is increased, and the abundance of beneficial
obligate anaerobes (Clostridia and families of this class) that are not capable of bacterial
translocation is decreased
7) the Firmicutes phylum is represented by 2 main classes Bacilli and Clostridia, which change in
different directions in cirrhosis and have different role in the gut microbiota; a meta-analysis of
the abundance of these taxa should be conducted and this should be indicated in the Discussion.
Title: Associations between changes in the gut microbiota and liver cirrhosis: a systematic
review and meta-analysis
Version: 0 Date: 11 Oct 2024
Reviewer's report:
This is an interesting systematic review, worthy of publication after correction
1) the abstract should not describe in detail the biodiversity indices, especially with numerical
characteristics - this is not interesting;
2) the abstract should indicate as many taxa as possible that showed significant and insignificant
differences with the control groups, especially at the level of higher taxa;
3) the conclusion indicates minor taxa that have a minimal effect on the macroorganism and are
unlikely to be useful in the  analysis of cirrhosis; authors have  good data on the differential
content of main taxa were and should indicate them  in the conclusions (without numerical
characteristics);
4) intestinal microbiota cannot be used to differentiate patients with cirrhosis from healthy
individuals - there are simpler methods for this; it could be useful in differentiating compensated
cirrhosis from chronic liver diseases at the pre-cirrhotic stage, but this was not studied in your
work, so this statement should be excluded;
5) a number of authors (https://pubmed.ncbi.nlm.nih.gov/34131470/; https://
pubmed.ncbi.nlm.nih.gov/24374295/) proposed dysbiosis ratios for the analysis of gut
microbiota in cirrhosis; this should be discussed based on your data;
6) it should be noted that in cirrhosis, the abundance of taxa of harmful facultative anaerobes
(Bacilli; Proteobacteria; families of these taxa) that are able to survive in oxygenated tissues
and are therefore capable of bacterial translocation is increased, and the abundance of beneficial
obligate anaerobes (Clostridia and families of this class) that are not capable of bacterial
translocation is decreased
7) the Firmicutes phylum is represented by 2 main classes Bacilli and Clostridia, which change in
different directions in cirrhosis and have different role in the gut microbiota; a meta-analysis of
the abundance of these taxa should be conducted and this should be indicated in the Discussion.
Please review this paper.
user
Title: Associations between changes in the gut microbiota and liver cirrhosis: a systematic
review and meta-analysis
Version: 0 Date: 11 Oct 2024
Reviewer's report:
This is an interesting systematic review, worthy of publication after correction
1) the abstract should not describe in detail the biodiversity indices, especially with numerical
characteristics - this is not interesting;
2) the abstract should indicate as many taxa as possible that showed significant and insignificant
differences with the control groups, especially at the level of higher taxa;
3) the conclusion indicates minor taxa that have a minimal effect on the macroorganism and are
unlikely to be useful in the  analysis of cirrhosis; authors have  good data on the differential
content of main taxa were and should indicate them  in the conclusions (without numerical
characteristics);
4) intestinal microbiota cannot be used to differentiate patients with cirrhosis from healthy
individuals - there are simpler methods for this; it could be useful in differentiating compensated
cirrhosis from chronic liver diseases at the pre-cirrhotic stage, but this was not studied in your
work, so this statement should be excluded;
5) a number of authors (https://pubmed.ncbi.nlm.nih.gov/34131470/; https://
pubmed.ncbi.nlm.nih.gov/24374295/) proposed dysbiosis ratios for the analysis of gut
microbiota in cirrhosis; this should be discussed based on your data;
6) it should be noted that in cirrhosis, the abundance of taxa of harmful facultative anaerobes
(Bacilli; Proteobacteria; families of these taxa) that are able to survive in oxygenated tissues
and are therefore capable of bacterial translocation is increased, and the abundance of beneficial
obligate anaerobes (Clostridia and families of this class) that are not capable of bacterial
translocation is decreased
7) the Firmicutes phylum is represented by 2 main classes Bacilli and Clostridia, which change in
different directions in cirrhosis and have different role in the gut microbiota; a meta-analysis of
the abundance of these taxa should be conducted and this should be indicated in the Discussion.
assistant