Reviewer's report 
 Cyclin A and cyclin D as significant prognostic markers in colorectal patients. Title: 
 1  4 June 2004 Version: Date: 
 Masao Miyashita Reviewer: 
Reviewer's report: 
General 
As authors mentioned as reference papers,there are several papers that reported similar results to 
this present paper submitted here. However, since authors investigated some molecular markers as 
cell cycle regulators, it is worthy to be published here. However, there are several points to be 
revised or to be structurally re-organized. After these problems are all cleared, this paper will be 
suitable to be accepted for publication. ------------------------------------------------------------------------------- Major Compulsory Revisions (that the author must respond to before a decision on publication can 
be reached) 
1) Authors must verify whether a selection of autosy samples as a negative control is suitable to the 
present study using fresh surgical samples because the time from death to sampling at autopsy may 
possibly affect the results. 
2) Authors must describe the detection rates of each staining in the results section before discussing 
the detection rates of cyclin D1 (68%) and cyclin A (80%) in discussion described in p11, 13, 
resepectively. 
------------------------------------------------------------------------------- Minor Essential Revisions (such as missing labels on figures, or the wrong use of a term, which the author can be trusted to correct) 
1) Figure numbers must be corrected. 2) Errors of words must be corrected. 
allover (all over)(p2, line 1), Ppoorly (Poorly)(p8,line 13), whether (either)(p8, line20),Ki-67 was 
(Ki-67and histone H3 were)(p9, line 11), Utsonomia (Utsunomia) (p11,line 14), the the (the)(p12, line 
13) 
------------------------------------------------------------------------------- Discretionary Revisions (which the author can choose to ignore) 
In results, the description of cyclin D1 gene amplification (cyclin D1 amplification V.S. staining, 
pathological data, prognosis) is confusing. Because these are the most important and central results in this study, these need to be summarized in Table. 
 Unable to decide on acceptance or rejection until the authors have responded to the What next?: 
major compulsory revisions 
 An article of importance in its field Level of interest: 
 Needs some language corrections before being published Quality of written English: 
 No Statistical review: 
Declaration of competing interests: 
None 
Reviewer's report 
 Cyclin A and cyclin D as significant prognostic markers in colorectal patients. Title: 
 1  4 June 2004 Version: Date: 
 Masao Miyashita Reviewer: 
Reviewer's report: 
General 
As authors mentioned as reference papers,there are several papers that reported similar results to 
this present paper submitted here. However, since authors investigated some molecular markers as 
cell cycle regulators, it is worthy to be published here. However, there are several points to be 
revised or to be structurally re-organized. After these problems are all cleared, this paper will be 
suitable to be accepted for publication. ------------------------------------------------------------------------------- Major Compulsory Revisions (that the author must respond to before a decision on publication can 
be reached) 
1) Authors must verify whether a selection of autosy samples as a negative control is suitable to the 
present study using fresh surgical samples because the time from death to sampling at autopsy may 
possibly affect the results. 
2) Authors must describe the detection rates of each staining in the results section before discussing 
the detection rates of cyclin D1 (68%) and cyclin A (80%) in discussion described in p11, 13, 
resepectively. 
------------------------------------------------------------------------------- Minor Essential Revisions (such as missing labels on figures, or the wrong use of a term, which the author can be trusted to correct) 
1) Figure numbers must be corrected. 2) Errors of words must be corrected. 
allover (all over)(p2, line 1), Ppoorly (Poorly)(p8,line 13), whether (either)(p8, line20),Ki-67 was 
(Ki-67and histone H3 were)(p9, line 11), Utsonomia (Utsunomia) (p11,line 14), the the (the)(p12, line 
13) 
------------------------------------------------------------------------------- Discretionary Revisions (which the author can choose to ignore) 
In results, the description of cyclin D1 gene amplification (cyclin D1 amplification V.S. staining, 
pathological data, prognosis) is confusing. Because these are the most important and central results in this study, these need to be summarized in Table. 
 Unable to decide on acceptance or rejection until the authors have responded to the What next?: 
major compulsory revisions 
 An article of importance in its field Level of interest: 
 Needs some language corrections before being published Quality of written English: 
 No Statistical review: 
Declaration of competing interests: 
None 
Please review this paper.
user
Reviewer's report 
 Cyclin A and cyclin D as significant prognostic markers in colorectal patients. Title: 
 1  4 June 2004 Version: Date: 
 Masao Miyashita Reviewer: 
Reviewer's report: 
General 
As authors mentioned as reference papers,there are several papers that reported similar results to 
this present paper submitted here. However, since authors investigated some molecular markers as 
cell cycle regulators, it is worthy to be published here. However, there are several points to be 
revised or to be structurally re-organized. After these problems are all cleared, this paper will be 
suitable to be accepted for publication. ------------------------------------------------------------------------------- Major Compulsory Revisions (that the author must respond to before a decision on publication can 
be reached) 
1) Authors must verify whether a selection of autosy samples as a negative control is suitable to the 
present study using fresh surgical samples because the time from death to sampling at autopsy may 
possibly affect the results. 
2) Authors must describe the detection rates of each staining in the results section before discussing 
the detection rates of cyclin D1 (68%) and cyclin A (80%) in discussion described in p11, 13, 
resepectively. 
------------------------------------------------------------------------------- Minor Essential Revisions (such as missing labels on figures, or the wrong use of a term, which the author can be trusted to correct) 
1) Figure numbers must be corrected. 2) Errors of words must be corrected. 
allover (all over)(p2, line 1), Ppoorly (Poorly)(p8,line 13), whether (either)(p8, line20),Ki-67 was 
(Ki-67and histone H3 were)(p9, line 11), Utsonomia (Utsunomia) (p11,line 14), the the (the)(p12, line 
13) 
------------------------------------------------------------------------------- Discretionary Revisions (which the author can choose to ignore) 
In results, the description of cyclin D1 gene amplification (cyclin D1 amplification V.S. staining, 
pathological data, prognosis) is confusing. Because these are the most important and central results in this study, these need to be summarized in Table. 
 Unable to decide on acceptance or rejection until the authors have responded to the What next?: 
major compulsory revisions 
 An article of importance in its field Level of interest: 
 Needs some language corrections before being published Quality of written English: 
 No Statistical review: 
Declaration of competing interests: 
None 
assistant