Reviewer's report
Title:Role of inducible nitric oxide synthase pathway on methotrexate-induced
intestinal mucositis in rodents
Version: 1Date:26 May 2011
Reviewer: jun kobayashi
Reviewer's report:
The authors showed that NOS inhibitors such as aminoguanidine and L-NAME
prevented MTX-induced intestinal damages by inhibiting intestinal iNOS
expressions and subsequent nitrosative stresses and MPO activities. The
experimental designs, the methods, and the results are all easy to understand,
suggesting a possibility to extend an investigation to developing promising
therapeutic solution in this field.
Major Compulsory Revisions:
(1) The descriptions for Fig.4 and Fig.5 in the results and legends does not
match Figs (4 and 5) at all and not enough for all figures. Please check and
rewrite.
(2) The authors showed MTX-induced protein levels of intestinal iNOS. Did you
measure iNOS activities? For example, How about intestinal tissue levels of
nitrite and nitrate.
(3) Where the origin of iNOS? Is it from enterocytes or circulating leukocytes?
(4) Different from duodenum and jejunum, there is no beneficial effect of L-NAME
treatment on villus height recovery in ileum, why?
Minor Essential Revisions:
There are a couple of miss spells in this manuscript.
(1) Introduction, paragraph 4, line 6 from bottom.
nitrotirosine------nitrotyrosine
(2) Discussion, last paragraph, line 9 from bottom.
NOSi---------iNOS
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Reviewer's report
Title:Role of inducible nitric oxide synthase pathway on methotrexate-induced
intestinal mucositis in rodents
Version: 1Date:26 May 2011
Reviewer: jun kobayashi
Reviewer's report:
The authors showed that NOS inhibitors such as aminoguanidine and L-NAME
prevented MTX-induced intestinal damages by inhibiting intestinal iNOS
expressions and subsequent nitrosative stresses and MPO activities. The
experimental designs, the methods, and the results are all easy to understand,
suggesting a possibility to extend an investigation to developing promising
therapeutic solution in this field.
Major Compulsory Revisions:
(1) The descriptions for Fig.4 and Fig.5 in the results and legends does not
match Figs (4 and 5) at all and not enough for all figures. Please check and
rewrite.
(2) The authors showed MTX-induced protein levels of intestinal iNOS. Did you
measure iNOS activities? For example, How about intestinal tissue levels of
nitrite and nitrate.
(3) Where the origin of iNOS? Is it from enterocytes or circulating leukocytes?
(4) Different from duodenum and jejunum, there is no beneficial effect of L-NAME
treatment on villus height recovery in ileum, why?
Minor Essential Revisions:
There are a couple of miss spells in this manuscript.
(1) Introduction, paragraph 4, line 6 from bottom.
nitrotirosine------nitrotyrosine
(2) Discussion, last paragraph, line 9 from bottom.
NOSi---------iNOS
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Please review this paper.
user
Reviewer's report
Title:Role of inducible nitric oxide synthase pathway on methotrexate-induced
intestinal mucositis in rodents
Version: 1Date:26 May 2011
Reviewer: jun kobayashi
Reviewer's report:
The authors showed that NOS inhibitors such as aminoguanidine and L-NAME
prevented MTX-induced intestinal damages by inhibiting intestinal iNOS
expressions and subsequent nitrosative stresses and MPO activities. The
experimental designs, the methods, and the results are all easy to understand,
suggesting a possibility to extend an investigation to developing promising
therapeutic solution in this field.
Major Compulsory Revisions:
(1) The descriptions for Fig.4 and Fig.5 in the results and legends does not
match Figs (4 and 5) at all and not enough for all figures. Please check and
rewrite.
(2) The authors showed MTX-induced protein levels of intestinal iNOS. Did you
measure iNOS activities? For example, How about intestinal tissue levels of
nitrite and nitrate.
(3) Where the origin of iNOS? Is it from enterocytes or circulating leukocytes?
(4) Different from duodenum and jejunum, there is no beneficial effect of L-NAME
treatment on villus height recovery in ileum, why?
Minor Essential Revisions:
There are a couple of miss spells in this manuscript.
(1) Introduction, paragraph 4, line 6 from bottom.
nitrotirosine------nitrotyrosine
(2) Discussion, last paragraph, line 9 from bottom.
NOSi---------iNOS
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
assistant