Reviewer's report
Title:MiR-195 Affects Cell Migration and Cell Proliferation by Down-regulating
DIEXF in Hirschsprung's Disease
Version:1Date:14 December 2013
Reviewer: Alessio Pini Prato
Reviewer's report:
Methods section
1) The colonic sampling from patients with intussusception or incarcerated
nguinal hernia seemsto me definitely risky and unjustfied given the risk-benefit
ratio of this research indication.
2) Were biopsies full thickness or seromuscular? This is of utmost imortance to
understand the risk of the procedure, particularly in control subjects
Minor essential revision:
Results section:
1) Although this section is clear, I think that the Authors should provide more
information regarding patients with syndromic features (Down, syndrome, CCHS,
MW, WS4, etc) that could have a distinct genetic background.
Discretionary revisions:
1) It would be extremely interesting that the Authors try to address the possible
consequences in terms of tumor predisposition or protection of overexpression of
miR-195 as well as of the consequential down-regulation of DIEXF. In a few
words I would be interested in reading a speculative discussion with regard to the
long term consequences of these disturbed pathways in HSCR patients. In fact, a
recent paper (Oncogene 2013) demonstrated the oncosuppressor role of RET in
colorectal cancer and its relationship to tumor predisposition in HSCR patients.
What about miR-195 and other miRNAs?
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:MiR-195 Affects Cell Migration and Cell Proliferation by Down-regulating
DIEXF in Hirschsprung's Disease
Version:1Date:14 December 2013
Reviewer: Alessio Pini Prato
Reviewer's report:
Methods section
1) The colonic sampling from patients with intussusception or incarcerated
nguinal hernia seemsto me definitely risky and unjustfied given the risk-benefit
ratio of this research indication.
2) Were biopsies full thickness or seromuscular? This is of utmost imortance to
understand the risk of the procedure, particularly in control subjects
Minor essential revision:
Results section:
1) Although this section is clear, I think that the Authors should provide more
information regarding patients with syndromic features (Down, syndrome, CCHS,
MW, WS4, etc) that could have a distinct genetic background.
Discretionary revisions:
1) It would be extremely interesting that the Authors try to address the possible
consequences in terms of tumor predisposition or protection of overexpression of
miR-195 as well as of the consequential down-regulation of DIEXF. In a few
words I would be interested in reading a speculative discussion with regard to the
long term consequences of these disturbed pathways in HSCR patients. In fact, a
recent paper (Oncogene 2013) demonstrated the oncosuppressor role of RET in
colorectal cancer and its relationship to tumor predisposition in HSCR patients.
What about miR-195 and other miRNAs?
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:MiR-195 Affects Cell Migration and Cell Proliferation by Down-regulating
DIEXF in Hirschsprung's Disease
Version:1Date:14 December 2013
Reviewer: Alessio Pini Prato
Reviewer's report:
Methods section
1) The colonic sampling from patients with intussusception or incarcerated
nguinal hernia seemsto me definitely risky and unjustfied given the risk-benefit
ratio of this research indication.
2) Were biopsies full thickness or seromuscular? This is of utmost imortance to
understand the risk of the procedure, particularly in control subjects
Minor essential revision:
Results section:
1) Although this section is clear, I think that the Authors should provide more
information regarding patients with syndromic features (Down, syndrome, CCHS,
MW, WS4, etc) that could have a distinct genetic background.
Discretionary revisions:
1) It would be extremely interesting that the Authors try to address the possible
consequences in terms of tumor predisposition or protection of overexpression of
miR-195 as well as of the consequential down-regulation of DIEXF. In a few
words I would be interested in reading a speculative discussion with regard to the
long term consequences of these disturbed pathways in HSCR patients. In fact, a
recent paper (Oncogene 2013) demonstrated the oncosuppressor role of RET in
colorectal cancer and its relationship to tumor predisposition in HSCR patients.
What about miR-195 and other miRNAs?
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I declare that I have no competing interests
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