Title: Helicobacter pylori CagA+ strains modulate colorectal pathology by regulating intestinal
flora
Version: 1 Date: 02 Dec 2024
Reviewer's report:
The author demonstrated the role of HP CagA+ strains to modulate colorectal pathology
by regulating intestinal flora. It is interesting study, but there are several difficulties in this
manuscript.
1. There is no mention of what “GC” stands for.
2. The author states that HP CagA+ strains have an effect on intestinal bacteria, but HP CagA-
strains should be used as control.
3. The author indicates that HP CagA+ strains may cause abnormalities in the intestinal
microbiota and thus influence colon carcinogenesis. However, epidemiological studies need to
be cited to see if this is the case. Based on the data from the present study, it would be an
overstatement to refer to an association between HP CagA+ strains and colorectal cancer.
4. The author states that HP CagA+ strains cause abnormalities in the intestinal microbiota and
progress destruction of the intestinal barrier. However, data on the disruption of the intestinal
barrier are not included in the results of this study, and the author should not discuss this
conclusion.
5. The author has measured serum cytokines in mice infected with HP CagA+ strains. However,
cytokine expression in the colonic mucosa needs to be compared to controls in order to assess
colonic inflammation.
Title: Helicobacter pylori CagA+ strains modulate colorectal pathology by regulating intestinal
flora
Version: 1 Date: 02 Dec 2024
Reviewer's report:
The author demonstrated the role of HP CagA+ strains to modulate colorectal pathology
by regulating intestinal flora. It is interesting study, but there are several difficulties in this
manuscript.
1. There is no mention of what “GC” stands for.
2. The author states that HP CagA+ strains have an effect on intestinal bacteria, but HP CagA-
strains should be used as control.
3. The author indicates that HP CagA+ strains may cause abnormalities in the intestinal
microbiota and thus influence colon carcinogenesis. However, epidemiological studies need to
be cited to see if this is the case. Based on the data from the present study, it would be an
overstatement to refer to an association between HP CagA+ strains and colorectal cancer.
4. The author states that HP CagA+ strains cause abnormalities in the intestinal microbiota and
progress destruction of the intestinal barrier. However, data on the disruption of the intestinal
barrier are not included in the results of this study, and the author should not discuss this
conclusion.
5. The author has measured serum cytokines in mice infected with HP CagA+ strains. However,
cytokine expression in the colonic mucosa needs to be compared to controls in order to assess
colonic inflammation.
Please review this paper.
user
Title: Helicobacter pylori CagA+ strains modulate colorectal pathology by regulating intestinal
flora
Version: 1 Date: 02 Dec 2024
Reviewer's report:
The author demonstrated the role of HP CagA+ strains to modulate colorectal pathology
by regulating intestinal flora. It is interesting study, but there are several difficulties in this
manuscript.
1. There is no mention of what “GC” stands for.
2. The author states that HP CagA+ strains have an effect on intestinal bacteria, but HP CagA-
strains should be used as control.
3. The author indicates that HP CagA+ strains may cause abnormalities in the intestinal
microbiota and thus influence colon carcinogenesis. However, epidemiological studies need to
be cited to see if this is the case. Based on the data from the present study, it would be an
overstatement to refer to an association between HP CagA+ strains and colorectal cancer.
4. The author states that HP CagA+ strains cause abnormalities in the intestinal microbiota and
progress destruction of the intestinal barrier. However, data on the disruption of the intestinal
barrier are not included in the results of this study, and the author should not discuss this
conclusion.
5. The author has measured serum cytokines in mice infected with HP CagA+ strains. However,
cytokine expression in the colonic mucosa needs to be compared to controls in order to assess
colonic inflammation.
assistant