Reviewer's report
Title:Co-culture of primary human tumor hepatocytes from patients with
hepatocellular carcinoma with autologous peripheral blood mononuclear cells:
Study of their in vitro immunological interactions.
Version: 2Date:27 September 2012
Reviewer: Frank Tacke
Reviewer's report:
The authors have improved the manuscript and I acknowledge that they tried to
address all concerns that I raised in the first review.
To me, two points remain unclear from the responses to my comments:
1. The new figure 3 is not convincing, because two control conditions are lacking:
isotype control and positive control (T-cells/lymphocytes and macrophages,
respectively). Without these data, the "negative" staining is not interpretable.
2. I am furthermore not fully convinced by the MHC-II staining. However, I think it
should be easy to confirm this point by real-time PCR.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Co-culture of primary human tumor hepatocytes from patients with
hepatocellular carcinoma with autologous peripheral blood mononuclear cells:
Study of their in vitro immunological interactions.
Version: 2Date:27 September 2012
Reviewer: Frank Tacke
Reviewer's report:
The authors have improved the manuscript and I acknowledge that they tried to
address all concerns that I raised in the first review.
To me, two points remain unclear from the responses to my comments:
1. The new figure 3 is not convincing, because two control conditions are lacking:
isotype control and positive control (T-cells/lymphocytes and macrophages,
respectively). Without these data, the "negative" staining is not interpretable.
2. I am furthermore not fully convinced by the MHC-II staining. However, I think it
should be easy to confirm this point by real-time PCR.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Co-culture of primary human tumor hepatocytes from patients with
hepatocellular carcinoma with autologous peripheral blood mononuclear cells:
Study of their in vitro immunological interactions.
Version: 2Date:27 September 2012
Reviewer: Frank Tacke
Reviewer's report:
The authors have improved the manuscript and I acknowledge that they tried to
address all concerns that I raised in the first review.
To me, two points remain unclear from the responses to my comments:
1. The new figure 3 is not convincing, because two control conditions are lacking:
isotype control and positive control (T-cells/lymphocytes and macrophages,
respectively). Without these data, the "negative" staining is not interpretable.
2. I am furthermore not fully convinced by the MHC-II staining. However, I think it
should be easy to confirm this point by real-time PCR.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests
assistant