Title: Formation of non-cholesterol gallstones in populations within clonorchiasis endemic
regions is closely related to Clonorchis sinensis infection
Version: 2 Date: 09 Apr 2025
Reviewer's report:
Dear Authors,
Thank you for taking the time to revise the manuscript. I have a few suggestions that might
further enhance the quality of your work.
1. While this may be a matter of personal preference, I recommend making the title smoother.
2. Please rewrite the Ethics Statement and the Participants and Specimens (1.) section to improve
clarity. Please clarify how the patient sample groups were formed. In one study, one group of
patients was used, while in another study, a different group of patients was used.
3. Who was selected for the "healthy medical examiners" group?
4. Please create a separate paragraph detailing the limitations of this study. Additionally, provide
more information about the patients in the Materials and Methods section, including their
comorbidities such as smoking, alcohol use disorders, chronic liver diseases, and other relevant
conditions. Ensure this information is clearly presented.
5. Please clarify how the different types of stones were classified.
6. In my opinion, the authors should mention the following paper:
DOI: 10.1017/S0022149X22000773.
Because similar information was previously presented in the paper, the data stating, "Through
microscopical examination of C. sinensis eggs and the material around them in gallbladder
stones, bile sediments, and bile, we discovered that the C. sinensis eggs participated in the stone
formation (Fig. 3)," is not new. The authors should rewrite this paragraph in the discussion
section.
7. In my opinion, the authors should cite the work by Luo XB et al., titled "Survey on Clonorchis
sinensis infection and bile component of gallstone patients from the Pearl River Delta region in
Guangdong Province," in the context of the presented data: "The results showed that the GSI
patients' bile had lower levels of TBA and CHO than that of the GP patients (P < 0.05), as seen
in Fig. 5." Similar information was previously observed by Luo and co-authors.
8. Please highlight the novelty and medical significance of this research, given that the main
finding—that C. sinensis eggs are involved in gallbladder stone formation—has been previously
published.
Title: Formation of non-cholesterol gallstones in populations within clonorchiasis endemic
regions is closely related to Clonorchis sinensis infection
Version: 2 Date: 09 Apr 2025
Reviewer's report:
Dear Authors,
Thank you for taking the time to revise the manuscript. I have a few suggestions that might
further enhance the quality of your work.
1. While this may be a matter of personal preference, I recommend making the title smoother.
2. Please rewrite the Ethics Statement and the Participants and Specimens (1.) section to improve
clarity. Please clarify how the patient sample groups were formed. In one study, one group of
patients was used, while in another study, a different group of patients was used.
3. Who was selected for the "healthy medical examiners" group?
4. Please create a separate paragraph detailing the limitations of this study. Additionally, provide
more information about the patients in the Materials and Methods section, including their
comorbidities such as smoking, alcohol use disorders, chronic liver diseases, and other relevant
conditions. Ensure this information is clearly presented.
5. Please clarify how the different types of stones were classified.
6. In my opinion, the authors should mention the following paper:
DOI: 10.1017/S0022149X22000773.
Because similar information was previously presented in the paper, the data stating, "Through
microscopical examination of C. sinensis eggs and the material around them in gallbladder
stones, bile sediments, and bile, we discovered that the C. sinensis eggs participated in the stone
formation (Fig. 3)," is not new. The authors should rewrite this paragraph in the discussion
section.
7. In my opinion, the authors should cite the work by Luo XB et al., titled "Survey on Clonorchis
sinensis infection and bile component of gallstone patients from the Pearl River Delta region in
Guangdong Province," in the context of the presented data: "The results showed that the GSI
patients' bile had lower levels of TBA and CHO than that of the GP patients (P < 0.05), as seen
in Fig. 5." Similar information was previously observed by Luo and co-authors.
8. Please highlight the novelty and medical significance of this research, given that the main
finding—that C. sinensis eggs are involved in gallbladder stone formation—has been previously
published.
Please review this paper.
user
Title: Formation of non-cholesterol gallstones in populations within clonorchiasis endemic
regions is closely related to Clonorchis sinensis infection
Version: 2 Date: 09 Apr 2025
Reviewer's report:
Dear Authors,
Thank you for taking the time to revise the manuscript. I have a few suggestions that might
further enhance the quality of your work.
1. While this may be a matter of personal preference, I recommend making the title smoother.
2. Please rewrite the Ethics Statement and the Participants and Specimens (1.) section to improve
clarity. Please clarify how the patient sample groups were formed. In one study, one group of
patients was used, while in another study, a different group of patients was used.
3. Who was selected for the "healthy medical examiners" group?
4. Please create a separate paragraph detailing the limitations of this study. Additionally, provide
more information about the patients in the Materials and Methods section, including their
comorbidities such as smoking, alcohol use disorders, chronic liver diseases, and other relevant
conditions. Ensure this information is clearly presented.
5. Please clarify how the different types of stones were classified.
6. In my opinion, the authors should mention the following paper:
DOI: 10.1017/S0022149X22000773.
Because similar information was previously presented in the paper, the data stating, "Through
microscopical examination of C. sinensis eggs and the material around them in gallbladder
stones, bile sediments, and bile, we discovered that the C. sinensis eggs participated in the stone
formation (Fig. 3)," is not new. The authors should rewrite this paragraph in the discussion
section.
7. In my opinion, the authors should cite the work by Luo XB et al., titled "Survey on Clonorchis
sinensis infection and bile component of gallstone patients from the Pearl River Delta region in
Guangdong Province," in the context of the presented data: "The results showed that the GSI
patients' bile had lower levels of TBA and CHO than that of the GP patients (P < 0.05), as seen
in Fig. 5." Similar information was previously observed by Luo and co-authors.
8. Please highlight the novelty and medical significance of this research, given that the main
finding—that C. sinensis eggs are involved in gallbladder stone formation—has been previously
published.
assistant