Reviewer's report
 Electron Paramagnetic Resonance Spectroscopic Studies Of Intestinal Homogenates From Title:
NSAIDs Treated Rats.
Authors:
Sivagurunathan Somasundaram (sivasoma@med.unc.edu )
Jasvinder Shergill (j_shergill@kcl.ac.uk )
John Wrigglesworth (john-wrigglesworth@kcl.ac.uk )
Sue Rafi (s_rafi@kcl.ac.uk )
Ingvar Bjarnason (ingvar_bjarnason@kcl.ac.uk )
 2  18 Mar 2002 Version: Date:
 Dr BL Tepperman Reviewer:
 A paper of limited interest Level of interest:
 Unable to decide on acceptance or rejection until the authors have responded Advice on publication:
to the compulsory revisions
I have a few concerns with the present study. 
1) The data are achieved using only single dosage regimens of indomethacin. If as the authors 
maintain, doses of indomethacin higher than 500 uM inhibit electron transport, then this should be also 
shown for the intestine. 
2) The role of NO is inferred from the use of an iNOS inhibitor. NO should be measured or at least the 
effect confirmed using a standard NO scavenger. The formation of nitrosy complexes could be 
confirmed using nitrotyrosine staining. 
3)Is it possible to quantify the EPR spectra. Examination of the spectra in Figures 1-5 is quite difficult especially without any scale and for a non-expert in the field. THis is the only way a reader may 
appreciate the impact of the procedure on electron transport in response to NSAID treatment. 
Competing interests:
None declared.
Reviewer's report
 Electron Paramagnetic Resonance Spectroscopic Studies Of Intestinal Homogenates From Title:
NSAIDs Treated Rats.
Authors:
Sivagurunathan Somasundaram (sivasoma@med.unc.edu )
Jasvinder Shergill (j_shergill@kcl.ac.uk )
John Wrigglesworth (john-wrigglesworth@kcl.ac.uk )
Sue Rafi (s_rafi@kcl.ac.uk )
Ingvar Bjarnason (ingvar_bjarnason@kcl.ac.uk )
 2  18 Mar 2002 Version: Date:
 Dr BL Tepperman Reviewer:
 A paper of limited interest Level of interest:
 Unable to decide on acceptance or rejection until the authors have responded Advice on publication:
to the compulsory revisions
I have a few concerns with the present study. 
1) The data are achieved using only single dosage regimens of indomethacin. If as the authors 
maintain, doses of indomethacin higher than 500 uM inhibit electron transport, then this should be also 
shown for the intestine. 
2) The role of NO is inferred from the use of an iNOS inhibitor. NO should be measured or at least the 
effect confirmed using a standard NO scavenger. The formation of nitrosy complexes could be 
confirmed using nitrotyrosine staining. 
3)Is it possible to quantify the EPR spectra. Examination of the spectra in Figures 1-5 is quite difficult especially without any scale and for a non-expert in the field. THis is the only way a reader may 
appreciate the impact of the procedure on electron transport in response to NSAID treatment. 
Competing interests:
None declared.
Please review this paper.
user
Reviewer's report
 Electron Paramagnetic Resonance Spectroscopic Studies Of Intestinal Homogenates From Title:
NSAIDs Treated Rats.
Authors:
Sivagurunathan Somasundaram (sivasoma@med.unc.edu )
Jasvinder Shergill (j_shergill@kcl.ac.uk )
John Wrigglesworth (john-wrigglesworth@kcl.ac.uk )
Sue Rafi (s_rafi@kcl.ac.uk )
Ingvar Bjarnason (ingvar_bjarnason@kcl.ac.uk )
 2  18 Mar 2002 Version: Date:
 Dr BL Tepperman Reviewer:
 A paper of limited interest Level of interest:
 Unable to decide on acceptance or rejection until the authors have responded Advice on publication:
to the compulsory revisions
I have a few concerns with the present study. 
1) The data are achieved using only single dosage regimens of indomethacin. If as the authors 
maintain, doses of indomethacin higher than 500 uM inhibit electron transport, then this should be also 
shown for the intestine. 
2) The role of NO is inferred from the use of an iNOS inhibitor. NO should be measured or at least the 
effect confirmed using a standard NO scavenger. The formation of nitrosy complexes could be 
confirmed using nitrotyrosine staining. 
3)Is it possible to quantify the EPR spectra. Examination of the spectra in Figures 1-5 is quite difficult especially without any scale and for a non-expert in the field. THis is the only way a reader may 
appreciate the impact of the procedure on electron transport in response to NSAID treatment. 
Competing interests:
None declared.
assistant