Title: Biochemical and histopathological evidence for beneficial effects of empagliflozin
pretreatment on acetic acid-induced colitis in rats
Version: 3 Date: 02 Mar 2023
Reviewer:  Paul Aimé Noubissi
Reviewer's report:
Biochemical and histopathological evidence for beneficial effects of empagliflozin pretreatment
on acetic acid-induced colitis in rats
Background: Ulcerative Colitis (UC) is a chronic disorder with oxidative stress playing a critical
role in its pathogenesis via upregulation of pro-inflammatory markers in colonic tissues. .
Results:  write “Furthermore, a significant increase in catalase (P<0.01), superoxide dismutase
(p<0.001), and glutathione peroxidase (p<0.01) activities was observed by EMPA pretreatment
when compared to AA group. These results were in line with those of the reference drug.”
Not
“Furthermore, a significant increase in catalase (P<0.01), superoxide dismutase (p<0.001),
and glutathione peroxidase (p<0.01) activities were observed by EMPA pretreatment when
compared to AA group. These results were in line with the results of the reference drug.”
Conclusions:
From the abstract conclusion, one can think the only ulcerative colitis symptom is the oxidative
stress. What about the colon weight/length ratio, macroscopic and histological score?
Background
Write “Investigations reported the potential anti-inflammatory, anti-oxidative and anti-apoptotic
properties of EMPA which could also exert possible beneficial effects on colitis.” rather
than “Investigations reported the potential anti-inflammatory, anti-oxidative and anti-apoptotic
properties of EMPA that could exert its possible beneficial effects on colitis too”
Experimental design
Rephrase this statement “(3) EMPA 10 mg/kg administered once per day treated group in AA-
induced colitis rats (AA+ EMPA); (4) Dexa 8 mg/kg per day administered group in AA-induced
colitis rats (AA + Dexa).”
Evaluation of antioxidant enzyme activity and oxidative stress biomarkers
Write “In brief, to 0.1 ml of tissue homogenate were added 0.9 ml of 1.8% sodium dodecyl
sulfate (SDS), 1.5 ml of 20% acetic acid solution (pH 3.5), and 1.5 ml of aqueous solution of
Thio barbituric acid.”
rather than
“In brief, 0.1 ml of tissue homogenates were added 0.9 ml of 1.8% sodium dodecyl sulfate
(SDS), 1.5 ml of 20% acetic acid solution (pH 3.5), and 1.5 ml of aqueous solution of Thio
barbituric acid.”
95oC correct to 95°C
Effect of empagliflozin on oxidative stress and antioxidant activity
- It is highly recommended to add some digital results rather than elastic sentences. Don’t just
write … significantly lower, significantly decreased, significantly increased, …. Write more
digital results.
Title: Biochemical and histopathological evidence for beneficial effects of empagliflozin
pretreatment on acetic acid-induced colitis in rats
Version: 3 Date: 02 Mar 2023
Reviewer:  Paul Aimé Noubissi
Reviewer's report:
Biochemical and histopathological evidence for beneficial effects of empagliflozin pretreatment
on acetic acid-induced colitis in rats
Background: Ulcerative Colitis (UC) is a chronic disorder with oxidative stress playing a critical
role in its pathogenesis via upregulation of pro-inflammatory markers in colonic tissues. .
Results:  write “Furthermore, a significant increase in catalase (P<0.01), superoxide dismutase
(p<0.001), and glutathione peroxidase (p<0.01) activities was observed by EMPA pretreatment
when compared to AA group. These results were in line with those of the reference drug.”
Not
“Furthermore, a significant increase in catalase (P<0.01), superoxide dismutase (p<0.001),
and glutathione peroxidase (p<0.01) activities were observed by EMPA pretreatment when
compared to AA group. These results were in line with the results of the reference drug.”
Conclusions:
From the abstract conclusion, one can think the only ulcerative colitis symptom is the oxidative
stress. What about the colon weight/length ratio, macroscopic and histological score?
Background
Write “Investigations reported the potential anti-inflammatory, anti-oxidative and anti-apoptotic
properties of EMPA which could also exert possible beneficial effects on colitis.” rather
than “Investigations reported the potential anti-inflammatory, anti-oxidative and anti-apoptotic
properties of EMPA that could exert its possible beneficial effects on colitis too”
Experimental design
Rephrase this statement “(3) EMPA 10 mg/kg administered once per day treated group in AA-
induced colitis rats (AA+ EMPA); (4) Dexa 8 mg/kg per day administered group in AA-induced
colitis rats (AA + Dexa).”
Evaluation of antioxidant enzyme activity and oxidative stress biomarkers
Write “In brief, to 0.1 ml of tissue homogenate were added 0.9 ml of 1.8% sodium dodecyl
sulfate (SDS), 1.5 ml of 20% acetic acid solution (pH 3.5), and 1.5 ml of aqueous solution of
Thio barbituric acid.”
rather than
“In brief, 0.1 ml of tissue homogenates were added 0.9 ml of 1.8% sodium dodecyl sulfate
(SDS), 1.5 ml of 20% acetic acid solution (pH 3.5), and 1.5 ml of aqueous solution of Thio
barbituric acid.”
95oC correct to 95°C
Effect of empagliflozin on oxidative stress and antioxidant activity
- It is highly recommended to add some digital results rather than elastic sentences. Don’t just
write … significantly lower, significantly decreased, significantly increased, …. Write more
digital results.
Please review this paper.
user
Title: Biochemical and histopathological evidence for beneficial effects of empagliflozin
pretreatment on acetic acid-induced colitis in rats
Version: 3 Date: 02 Mar 2023
Reviewer:  Paul Aimé Noubissi
Reviewer's report:
Biochemical and histopathological evidence for beneficial effects of empagliflozin pretreatment
on acetic acid-induced colitis in rats
Background: Ulcerative Colitis (UC) is a chronic disorder with oxidative stress playing a critical
role in its pathogenesis via upregulation of pro-inflammatory markers in colonic tissues. .
Results:  write “Furthermore, a significant increase in catalase (P<0.01), superoxide dismutase
(p<0.001), and glutathione peroxidase (p<0.01) activities was observed by EMPA pretreatment
when compared to AA group. These results were in line with those of the reference drug.”
Not
“Furthermore, a significant increase in catalase (P<0.01), superoxide dismutase (p<0.001),
and glutathione peroxidase (p<0.01) activities were observed by EMPA pretreatment when
compared to AA group. These results were in line with the results of the reference drug.”
Conclusions:
From the abstract conclusion, one can think the only ulcerative colitis symptom is the oxidative
stress. What about the colon weight/length ratio, macroscopic and histological score?
Background
Write “Investigations reported the potential anti-inflammatory, anti-oxidative and anti-apoptotic
properties of EMPA which could also exert possible beneficial effects on colitis.” rather
than “Investigations reported the potential anti-inflammatory, anti-oxidative and anti-apoptotic
properties of EMPA that could exert its possible beneficial effects on colitis too”
Experimental design
Rephrase this statement “(3) EMPA 10 mg/kg administered once per day treated group in AA-
induced colitis rats (AA+ EMPA); (4) Dexa 8 mg/kg per day administered group in AA-induced
colitis rats (AA + Dexa).”
Evaluation of antioxidant enzyme activity and oxidative stress biomarkers
Write “In brief, to 0.1 ml of tissue homogenate were added 0.9 ml of 1.8% sodium dodecyl
sulfate (SDS), 1.5 ml of 20% acetic acid solution (pH 3.5), and 1.5 ml of aqueous solution of
Thio barbituric acid.”
rather than
“In brief, 0.1 ml of tissue homogenates were added 0.9 ml of 1.8% sodium dodecyl sulfate
(SDS), 1.5 ml of 20% acetic acid solution (pH 3.5), and 1.5 ml of aqueous solution of Thio
barbituric acid.”
95oC correct to 95°C
Effect of empagliflozin on oxidative stress and antioxidant activity
- It is highly recommended to add some digital results rather than elastic sentences. Don’t just
write … significantly lower, significantly decreased, significantly increased, …. Write more
digital results.
assistant