Title: Formation of non-cholesterol gallstones in populations within clonorchiasis endemic
regions is closely related to Clonorchis sinensis infection
Version: 1 Date: 07 Mar 2025
Reviewer's report:
Dear Authors,
In my opinion, this is a very well-conducted and important study. However, I have some
suggestions for improvement.
Major:
1. Did men under 50 years of age in the highest risk group have concomitant diseases that could
affect the condition of the gallbladder? This includes conditions such as smoking, alcohol use
disorder, metabolic syndrome, type 2 diabetes, etc.
2. Could the lack of reliable results in women be attributed to a smaller sample size compared
to men?
3. Is it possible to calculate the correlation between the number of non-viable or dead eggs and
the severity of gallstone disease in men under 50 years of age with combined Clonorchis sinensis
infection? This could potentially serve as additional evidence.
4. Please include a separate discussion point addressing the clear potential mechanism of
gallbladder stone formation under C. sinensis infection.
Minor:
1. In the Introduction section, please provide supporting data for the statement: "Nevertheless,
little information on the impact of C. sinensis infection on the composition of bile and the
function of the gallbladder has been reported thus far."
2. Please include the ethical approval number and the date of approval in the text.
Title: Formation of non-cholesterol gallstones in populations within clonorchiasis endemic
regions is closely related to Clonorchis sinensis infection
Version: 1 Date: 07 Mar 2025
Reviewer's report:
Dear Authors,
In my opinion, this is a very well-conducted and important study. However, I have some
suggestions for improvement.
Major:
1. Did men under 50 years of age in the highest risk group have concomitant diseases that could
affect the condition of the gallbladder? This includes conditions such as smoking, alcohol use
disorder, metabolic syndrome, type 2 diabetes, etc.
2. Could the lack of reliable results in women be attributed to a smaller sample size compared
to men?
3. Is it possible to calculate the correlation between the number of non-viable or dead eggs and
the severity of gallstone disease in men under 50 years of age with combined Clonorchis sinensis
infection? This could potentially serve as additional evidence.
4. Please include a separate discussion point addressing the clear potential mechanism of
gallbladder stone formation under C. sinensis infection.
Minor:
1. In the Introduction section, please provide supporting data for the statement: "Nevertheless,
little information on the impact of C. sinensis infection on the composition of bile and the
function of the gallbladder has been reported thus far."
2. Please include the ethical approval number and the date of approval in the text.
Please review this paper.
user
Title: Formation of non-cholesterol gallstones in populations within clonorchiasis endemic
regions is closely related to Clonorchis sinensis infection
Version: 1 Date: 07 Mar 2025
Reviewer's report:
Dear Authors,
In my opinion, this is a very well-conducted and important study. However, I have some
suggestions for improvement.
Major:
1. Did men under 50 years of age in the highest risk group have concomitant diseases that could
affect the condition of the gallbladder? This includes conditions such as smoking, alcohol use
disorder, metabolic syndrome, type 2 diabetes, etc.
2. Could the lack of reliable results in women be attributed to a smaller sample size compared
to men?
3. Is it possible to calculate the correlation between the number of non-viable or dead eggs and
the severity of gallstone disease in men under 50 years of age with combined Clonorchis sinensis
infection? This could potentially serve as additional evidence.
4. Please include a separate discussion point addressing the clear potential mechanism of
gallbladder stone formation under C. sinensis infection.
Minor:
1. In the Introduction section, please provide supporting data for the statement: "Nevertheless,
little information on the impact of C. sinensis infection on the composition of bile and the
function of the gallbladder has been reported thus far."
2. Please include the ethical approval number and the date of approval in the text.
assistant