Reviewer's report
Title:Antiulcer activity of fluvoxamine in rats and its effect on oxidant and
antioxidant parameters in stomach tissue
Version: 3Date:25 March 2009
Reviewer: Predrag Sikiric
Reviewer's report:
Generally, the manuscript is now considerably improved and all of my previous
comments were adequately solved.
However, the only point that I could object is the statement that ranitidine was
evaluated as an ulcerogenic agent (!) (i.e., Abstract:
„Methods: Groups of rats fasted for 24 h received fluvoxamine (25, 50, 100 and
200 mg/kg), ranitidine (50 mg/kg) or distilled water by oral gavage.
Indomethacin (25 mg/kg) was orally administered to the rats as an ulcerative
agent. Six hours after ulcer induction, the stomachs of the rats were excised and
an ulcer index determined. Separate groups of rats were treated with the same
doses of fluvoxamine and ranitidine, but not with indomethacin, to test for
ulcerative effects of these drugs alone. The stomachs were evaluated
biochemically to determine oxidant and antioxidant parameters. We used
one-way ANOVA and LSD options for data analysis. Results: The 25, 50, 100
and 200 mg/kg doses of fluvoxamine exerted antiulcer effects of 48.5, 67.5, 82.1
and 96.1%, respectively, compared to the control rat group. Ranitidine showed
an 86.5% antiulcer effect. No ulcers were observed in the absence of
indomethacin treatment for any dose of fluvoxamine or for ranitidine.
….“ Results: The antiulcer effect of 50 mg/kg ranitidine was 86.5%. No ulcers
were observed in the absence of indomethacin treatment following any dose of
fluvoxamine or ranitidine.“).
Thus, although the intention of the authors is clear, the idea that ranitidine may
induce ulcer by itself is quite unusual, and the above statements should be
adequately corrected.
The same goes for fluvoxamine. If they authors think that fluvoxamine may
produce ulcer by itself, and then, protect against indomethacin lesion, they
should provide some background (i.e., small irritant may protect against strong
irritant).
Finally, providing that this technical point could be solved without problem, I
maintained my previous high estimation of this very interesting manuscript that
deserves full attention and presentation.
Reviewer's report
Title:Antiulcer activity of fluvoxamine in rats and its effect on oxidant and
antioxidant parameters in stomach tissue
Version: 3Date:25 March 2009
Reviewer: Predrag Sikiric
Reviewer's report:
Generally, the manuscript is now considerably improved and all of my previous
comments were adequately solved.
However, the only point that I could object is the statement that ranitidine was
evaluated as an ulcerogenic agent (!) (i.e., Abstract:
„Methods: Groups of rats fasted for 24 h received fluvoxamine (25, 50, 100 and
200 mg/kg), ranitidine (50 mg/kg) or distilled water by oral gavage.
Indomethacin (25 mg/kg) was orally administered to the rats as an ulcerative
agent. Six hours after ulcer induction, the stomachs of the rats were excised and
an ulcer index determined. Separate groups of rats were treated with the same
doses of fluvoxamine and ranitidine, but not with indomethacin, to test for
ulcerative effects of these drugs alone. The stomachs were evaluated
biochemically to determine oxidant and antioxidant parameters. We used
one-way ANOVA and LSD options for data analysis. Results: The 25, 50, 100
and 200 mg/kg doses of fluvoxamine exerted antiulcer effects of 48.5, 67.5, 82.1
and 96.1%, respectively, compared to the control rat group. Ranitidine showed
an 86.5% antiulcer effect. No ulcers were observed in the absence of
indomethacin treatment for any dose of fluvoxamine or for ranitidine.
….“ Results: The antiulcer effect of 50 mg/kg ranitidine was 86.5%. No ulcers
were observed in the absence of indomethacin treatment following any dose of
fluvoxamine or ranitidine.“).
Thus, although the intention of the authors is clear, the idea that ranitidine may
induce ulcer by itself is quite unusual, and the above statements should be
adequately corrected.
The same goes for fluvoxamine. If they authors think that fluvoxamine may
produce ulcer by itself, and then, protect against indomethacin lesion, they
should provide some background (i.e., small irritant may protect against strong
irritant).
Finally, providing that this technical point could be solved without problem, I
maintained my previous high estimation of this very interesting manuscript that
deserves full attention and presentation.
Please review this paper.
user
Reviewer's report
Title:Antiulcer activity of fluvoxamine in rats and its effect on oxidant and
antioxidant parameters in stomach tissue
Version: 3Date:25 March 2009
Reviewer: Predrag Sikiric
Reviewer's report:
Generally, the manuscript is now considerably improved and all of my previous
comments were adequately solved.
However, the only point that I could object is the statement that ranitidine was
evaluated as an ulcerogenic agent (!) (i.e., Abstract:
„Methods: Groups of rats fasted for 24 h received fluvoxamine (25, 50, 100 and
200 mg/kg), ranitidine (50 mg/kg) or distilled water by oral gavage.
Indomethacin (25 mg/kg) was orally administered to the rats as an ulcerative
agent. Six hours after ulcer induction, the stomachs of the rats were excised and
an ulcer index determined. Separate groups of rats were treated with the same
doses of fluvoxamine and ranitidine, but not with indomethacin, to test for
ulcerative effects of these drugs alone. The stomachs were evaluated
biochemically to determine oxidant and antioxidant parameters. We used
one-way ANOVA and LSD options for data analysis. Results: The 25, 50, 100
and 200 mg/kg doses of fluvoxamine exerted antiulcer effects of 48.5, 67.5, 82.1
and 96.1%, respectively, compared to the control rat group. Ranitidine showed
an 86.5% antiulcer effect. No ulcers were observed in the absence of
indomethacin treatment for any dose of fluvoxamine or for ranitidine.
….“ Results: The antiulcer effect of 50 mg/kg ranitidine was 86.5%. No ulcers
were observed in the absence of indomethacin treatment following any dose of
fluvoxamine or ranitidine.“).
Thus, although the intention of the authors is clear, the idea that ranitidine may
induce ulcer by itself is quite unusual, and the above statements should be
adequately corrected.
The same goes for fluvoxamine. If they authors think that fluvoxamine may
produce ulcer by itself, and then, protect against indomethacin lesion, they
should provide some background (i.e., small irritant may protect against strong
irritant).
Finally, providing that this technical point could be solved without problem, I
maintained my previous high estimation of this very interesting manuscript that
deserves full attention and presentation.
assistant