Title: Administration of Probiotics to Healthy Volunteers – Effects on Reactivity of Intestinal
Mucosa and Systemic Leukocytes
Version: 4 Date: 21 Oct 2021
Reviewer:  Jayesh Ahire
Reviewer's report:
1. Line 60-62: Provide clarity to the sentences. After ingestion of what?
2. Line 62: change the sentence for better understanding, “Blood tests were sampled”
3. Study start date (January 2006)/actual primary completion date (March 2006) and actual study
completion (February 2012) dates are too old. Please justify the reason behind the delay (in
what?)? Publication?
4. Besides this, study indicated as retrospectively registered. Justify the reason in manuscript.
5. Provide statement in the manuscript that “All ongoing and related trials for this intervention
are registered”.
6. Line 113: Provide human ethics committee approval date.
7. Line 114: as per the clinical trial registration data, the dates appear different. Justify.
8. Line 125: correct sentence construction error.
9. Provide manufacturing details like batch number and date for probiotics used in this study.
10. Line 133: The gastrointestinal function scale is validated scale? Provide reference for the
same.
11. Line 242-243: The label claim is evaluated for the probiotic product? The product is stable at
room temperature or 4 oC. The required conditions were maintained to keep label claim strength
of the bacteria?
12. Line 314: Bifidobacteria capable to grow well in MRS supplemented with 0.1% L-Cysteine,
without cysteine it is hard to cultivate them.
13. Why placebo is not included in the study? In my opinion, placebo results are must to confirm
the finding and effects of probiotics.
14. It could be nice, if authors can add histology images.
15. Figure 2 a and b: properly label the “Y” axis.
Title: Administration of Probiotics to Healthy Volunteers – Effects on Reactivity of Intestinal
Mucosa and Systemic Leukocytes
Version: 4 Date: 21 Oct 2021
Reviewer:  Jayesh Ahire
Reviewer's report:
1. Line 60-62: Provide clarity to the sentences. After ingestion of what?
2. Line 62: change the sentence for better understanding, “Blood tests were sampled”
3. Study start date (January 2006)/actual primary completion date (March 2006) and actual study
completion (February 2012) dates are too old. Please justify the reason behind the delay (in
what?)? Publication?
4. Besides this, study indicated as retrospectively registered. Justify the reason in manuscript.
5. Provide statement in the manuscript that “All ongoing and related trials for this intervention
are registered”.
6. Line 113: Provide human ethics committee approval date.
7. Line 114: as per the clinical trial registration data, the dates appear different. Justify.
8. Line 125: correct sentence construction error.
9. Provide manufacturing details like batch number and date for probiotics used in this study.
10. Line 133: The gastrointestinal function scale is validated scale? Provide reference for the
same.
11. Line 242-243: The label claim is evaluated for the probiotic product? The product is stable at
room temperature or 4 oC. The required conditions were maintained to keep label claim strength
of the bacteria?
12. Line 314: Bifidobacteria capable to grow well in MRS supplemented with 0.1% L-Cysteine,
without cysteine it is hard to cultivate them.
13. Why placebo is not included in the study? In my opinion, placebo results are must to confirm
the finding and effects of probiotics.
14. It could be nice, if authors can add histology images.
15. Figure 2 a and b: properly label the “Y” axis.
Please review this paper.
user
Title: Administration of Probiotics to Healthy Volunteers – Effects on Reactivity of Intestinal
Mucosa and Systemic Leukocytes
Version: 4 Date: 21 Oct 2021
Reviewer:  Jayesh Ahire
Reviewer's report:
1. Line 60-62: Provide clarity to the sentences. After ingestion of what?
2. Line 62: change the sentence for better understanding, “Blood tests were sampled”
3. Study start date (January 2006)/actual primary completion date (March 2006) and actual study
completion (February 2012) dates are too old. Please justify the reason behind the delay (in
what?)? Publication?
4. Besides this, study indicated as retrospectively registered. Justify the reason in manuscript.
5. Provide statement in the manuscript that “All ongoing and related trials for this intervention
are registered”.
6. Line 113: Provide human ethics committee approval date.
7. Line 114: as per the clinical trial registration data, the dates appear different. Justify.
8. Line 125: correct sentence construction error.
9. Provide manufacturing details like batch number and date for probiotics used in this study.
10. Line 133: The gastrointestinal function scale is validated scale? Provide reference for the
same.
11. Line 242-243: The label claim is evaluated for the probiotic product? The product is stable at
room temperature or 4 oC. The required conditions were maintained to keep label claim strength
of the bacteria?
12. Line 314: Bifidobacteria capable to grow well in MRS supplemented with 0.1% L-Cysteine,
without cysteine it is hard to cultivate them.
13. Why placebo is not included in the study? In my opinion, placebo results are must to confirm
the finding and effects of probiotics.
14. It could be nice, if authors can add histology images.
15. Figure 2 a and b: properly label the “Y” axis.
assistant