Title: Single-cell transcriptomic analysis reveals prognosis-related stromal signatures that
potentiate stratification of patients with extrahepatic cholangiocarcinoma
Version: 0 Date: 12 Jan 2025
Reviewer:  Y. Huang
Reviewer's report:
The authors in this study profiled the tumor cellular composition and identified prognosis-related
stromal signatures by scRNA-seq in eCCA. The results revealed that the eCCA tumors were
categorized into three classes: proliferative Group 1, inflammatory and fibrotic Group 2, and
neuronal Group 3. Patients in Group 3 had a better prognosis when compared to Group 1 and 2,
reflecting different impact of stromal subtypes on tumor progression.
1. There were some reported researches by analysis the features of eCCA using the scRNA-seq,
What is the most important new findings in this study?
2. As discussed in this study, further studies are needed to determine the differences between
pCCA and dCCA.
3. As per the results in this study, what is the clinical benefit to a patient with eCCA? How do
the result stratify a eCCA patient with good or poor prognosis?
4. More discussion should address the future application prospects.
Title: Single-cell transcriptomic analysis reveals prognosis-related stromal signatures that
potentiate stratification of patients with extrahepatic cholangiocarcinoma
Version: 0 Date: 12 Jan 2025
Reviewer:  Y. Huang
Reviewer's report:
The authors in this study profiled the tumor cellular composition and identified prognosis-related
stromal signatures by scRNA-seq in eCCA. The results revealed that the eCCA tumors were
categorized into three classes: proliferative Group 1, inflammatory and fibrotic Group 2, and
neuronal Group 3. Patients in Group 3 had a better prognosis when compared to Group 1 and 2,
reflecting different impact of stromal subtypes on tumor progression.
1. There were some reported researches by analysis the features of eCCA using the scRNA-seq,
What is the most important new findings in this study?
2. As discussed in this study, further studies are needed to determine the differences between
pCCA and dCCA.
3. As per the results in this study, what is the clinical benefit to a patient with eCCA? How do
the result stratify a eCCA patient with good or poor prognosis?
4. More discussion should address the future application prospects.
Please review this paper.
user
Title: Single-cell transcriptomic analysis reveals prognosis-related stromal signatures that
potentiate stratification of patients with extrahepatic cholangiocarcinoma
Version: 0 Date: 12 Jan 2025
Reviewer:  Y. Huang
Reviewer's report:
The authors in this study profiled the tumor cellular composition and identified prognosis-related
stromal signatures by scRNA-seq in eCCA. The results revealed that the eCCA tumors were
categorized into three classes: proliferative Group 1, inflammatory and fibrotic Group 2, and
neuronal Group 3. Patients in Group 3 had a better prognosis when compared to Group 1 and 2,
reflecting different impact of stromal subtypes on tumor progression.
1. There were some reported researches by analysis the features of eCCA using the scRNA-seq,
What is the most important new findings in this study?
2. As discussed in this study, further studies are needed to determine the differences between
pCCA and dCCA.
3. As per the results in this study, what is the clinical benefit to a patient with eCCA? How do
the result stratify a eCCA patient with good or poor prognosis?
4. More discussion should address the future application prospects.
assistant