Reviewer's report
Title:Reduced diversity and increased virulence-gene carriage in intestinal
enterobacteria of coeliac children
Version: 1Date:15 June 2008
Reviewer: Christel Neut
Reviewer's report:
The presented work consists of research of virulence factors of E. coli isolates
from children suffering from coeliac disease. Virulent strains can be implicated in
the physiopathological conditions.
Different points need to be clarified (major compulsory revisions):
-The methods used enables the authors to outnumber enterobacteria, why no
quantitative results are shown? A higher amount of enterobacteria may exist in
CD children which would further increase the role of E. coli in pathological
conditions.
-Five colonies are subcultured for each sample. How is the diversity of
distribution of the virulence genes in these five subcultures. Are identical clones
present frequently. How are they treated further? The table 1 mentionned 17
clones in active CD patients. How can we relate this number to the 50 colonies
subcultured from 10 samples. When more than 3 clones can be identified per
sample, the number of subcultured colonies should be increased.
-Why the number of clones belonging the the B2 and D group is identical to the A
and B1 identical in the three groups (table 1)?
-The results in the figures 1 and 2 should be expressed in number of clones and
not percentages. So we can be able to better compare the diversity.
Only after clarification of the methodology, the discussion can be evaluated.
Minor revisions: spelling mistakes need to be removed.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Reduced diversity and increased virulence-gene carriage in intestinal
enterobacteria of coeliac children
Version: 1Date:15 June 2008
Reviewer: Christel Neut
Reviewer's report:
The presented work consists of research of virulence factors of E. coli isolates
from children suffering from coeliac disease. Virulent strains can be implicated in
the physiopathological conditions.
Different points need to be clarified (major compulsory revisions):
-The methods used enables the authors to outnumber enterobacteria, why no
quantitative results are shown? A higher amount of enterobacteria may exist in
CD children which would further increase the role of E. coli in pathological
conditions.
-Five colonies are subcultured for each sample. How is the diversity of
distribution of the virulence genes in these five subcultures. Are identical clones
present frequently. How are they treated further? The table 1 mentionned 17
clones in active CD patients. How can we relate this number to the 50 colonies
subcultured from 10 samples. When more than 3 clones can be identified per
sample, the number of subcultured colonies should be increased.
-Why the number of clones belonging the the B2 and D group is identical to the A
and B1 identical in the three groups (table 1)?
-The results in the figures 1 and 2 should be expressed in number of clones and
not percentages. So we can be able to better compare the diversity.
Only after clarification of the methodology, the discussion can be evaluated.
Minor revisions: spelling mistakes need to be removed.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Reduced diversity and increased virulence-gene carriage in intestinal
enterobacteria of coeliac children
Version: 1Date:15 June 2008
Reviewer: Christel Neut
Reviewer's report:
The presented work consists of research of virulence factors of E. coli isolates
from children suffering from coeliac disease. Virulent strains can be implicated in
the physiopathological conditions.
Different points need to be clarified (major compulsory revisions):
-The methods used enables the authors to outnumber enterobacteria, why no
quantitative results are shown? A higher amount of enterobacteria may exist in
CD children which would further increase the role of E. coli in pathological
conditions.
-Five colonies are subcultured for each sample. How is the diversity of
distribution of the virulence genes in these five subcultures. Are identical clones
present frequently. How are they treated further? The table 1 mentionned 17
clones in active CD patients. How can we relate this number to the 50 colonies
subcultured from 10 samples. When more than 3 clones can be identified per
sample, the number of subcultured colonies should be increased.
-Why the number of clones belonging the the B2 and D group is identical to the A
and B1 identical in the three groups (table 1)?
-The results in the figures 1 and 2 should be expressed in number of clones and
not percentages. So we can be able to better compare the diversity.
Only after clarification of the methodology, the discussion can be evaluated.
Minor revisions: spelling mistakes need to be removed.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
assistant