Reviewer's report
Title:Lubiprostone stimulates intestinal mucin release
Version: 2Date:6 August 2012
Reviewer: Ning Yang
Reviewer's report:
The work communicated by the author represents an extension to his previous
study about lubiprostone. In addition to chloride secretion, the present study
shows that lubiprostone also has prostaglandin-like actions on mucus release in
the mouse small intestine, which may be greater potential for the therapeutic
applications of lubiprostone.
Minor Essential Revisions
1. Background, first paragraph, please delete the “(CF)” in “cystic fibrosis (CF)
transmembrane conductance regulator (CFTR)”.
2. Mucus is a slippery discharge that comprised mucins, inorganic salts, water,
cells and so on, while mucins, a family of high molecular weight, heavily
glycosylated proteins, are the major components of mucus. In the present study,
the author were more likely measuring the relatively quantity of mucus instead of
mucins since the contents in either the standards or the perfusates are not pure
mucins.
3. In Materials and Methods, Measurement of intestinal mucin release using an
ex vivo system., please clarify how many segments were taken from each mouse
and how many mice were used in each group.
4. In Materials and Methods, Quantification of released mucins., were the sample
unknowns determined using the standard curve data from the same
nitrocellulose membrane every time? According my personal experience, the
color of the dried blots changes with even a tiny condition difference, such as
temperature, time. The dots of sample unknowns should be compared with the
dots of the standards under the same condition.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:Lubiprostone stimulates intestinal mucin release
Version: 2Date:6 August 2012
Reviewer: Ning Yang
Reviewer's report:
The work communicated by the author represents an extension to his previous
study about lubiprostone. In addition to chloride secretion, the present study
shows that lubiprostone also has prostaglandin-like actions on mucus release in
the mouse small intestine, which may be greater potential for the therapeutic
applications of lubiprostone.
Minor Essential Revisions
1. Background, first paragraph, please delete the “(CF)” in “cystic fibrosis (CF)
transmembrane conductance regulator (CFTR)”.
2. Mucus is a slippery discharge that comprised mucins, inorganic salts, water,
cells and so on, while mucins, a family of high molecular weight, heavily
glycosylated proteins, are the major components of mucus. In the present study,
the author were more likely measuring the relatively quantity of mucus instead of
mucins since the contents in either the standards or the perfusates are not pure
mucins.
3. In Materials and Methods, Measurement of intestinal mucin release using an
ex vivo system., please clarify how many segments were taken from each mouse
and how many mice were used in each group.
4. In Materials and Methods, Quantification of released mucins., were the sample
unknowns determined using the standard curve data from the same
nitrocellulose membrane every time? According my personal experience, the
color of the dried blots changes with even a tiny condition difference, such as
temperature, time. The dots of sample unknowns should be compared with the
dots of the standards under the same condition.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:Lubiprostone stimulates intestinal mucin release
Version: 2Date:6 August 2012
Reviewer: Ning Yang
Reviewer's report:
The work communicated by the author represents an extension to his previous
study about lubiprostone. In addition to chloride secretion, the present study
shows that lubiprostone also has prostaglandin-like actions on mucus release in
the mouse small intestine, which may be greater potential for the therapeutic
applications of lubiprostone.
Minor Essential Revisions
1. Background, first paragraph, please delete the “(CF)” in “cystic fibrosis (CF)
transmembrane conductance regulator (CFTR)”.
2. Mucus is a slippery discharge that comprised mucins, inorganic salts, water,
cells and so on, while mucins, a family of high molecular weight, heavily
glycosylated proteins, are the major components of mucus. In the present study,
the author were more likely measuring the relatively quantity of mucus instead of
mucins since the contents in either the standards or the perfusates are not pure
mucins.
3. In Materials and Methods, Measurement of intestinal mucin release using an
ex vivo system., please clarify how many segments were taken from each mouse
and how many mice were used in each group.
4. In Materials and Methods, Quantification of released mucins., were the sample
unknowns determined using the standard curve data from the same
nitrocellulose membrane every time? According my personal experience, the
color of the dried blots changes with even a tiny condition difference, such as
temperature, time. The dots of sample unknowns should be compared with the
dots of the standards under the same condition.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
assistant