Title: Evaluation of vital genes correlated with CD8+ T cell infiltration as prognostic biomarkers
in stomach adenocarcinoma
Version: 5 Date: 10 Oct 2023
Reviewer's report:
Thank you for addressing my comments. Additionally, please answer these comments below.
## It is very unfortunate that immunostaining cannot be performed. The results in the paper are
based only on gene expression level analysis and public database analysis, and it still remains
unclear whether this risk score truly correlates with CD8+TIL. Therefore, if you cannot perform
protein expression analysis such as immunostaining, please mention it in the limitation in the
discussion.
## Although these genes, EPS8L3, MUC13 and PLEKHS1, have been shown to be involved
with cancer cells, it remains unclear how they interact immunologically. Please add another
immunological discussion. If not, please state in the discussion that these mechanisms have not
been shown in the previous report.
I hope these comment will be helpful.
Title: Evaluation of vital genes correlated with CD8+ T cell infiltration as prognostic biomarkers
in stomach adenocarcinoma
Version: 5 Date: 10 Oct 2023
Reviewer's report:
Thank you for addressing my comments. Additionally, please answer these comments below.
## It is very unfortunate that immunostaining cannot be performed. The results in the paper are
based only on gene expression level analysis and public database analysis, and it still remains
unclear whether this risk score truly correlates with CD8+TIL. Therefore, if you cannot perform
protein expression analysis such as immunostaining, please mention it in the limitation in the
discussion.
## Although these genes, EPS8L3, MUC13 and PLEKHS1, have been shown to be involved
with cancer cells, it remains unclear how they interact immunologically. Please add another
immunological discussion. If not, please state in the discussion that these mechanisms have not
been shown in the previous report.
I hope these comment will be helpful.
Please review this paper.
user
Title: Evaluation of vital genes correlated with CD8+ T cell infiltration as prognostic biomarkers
in stomach adenocarcinoma
Version: 5 Date: 10 Oct 2023
Reviewer's report:
Thank you for addressing my comments. Additionally, please answer these comments below.
## It is very unfortunate that immunostaining cannot be performed. The results in the paper are
based only on gene expression level analysis and public database analysis, and it still remains
unclear whether this risk score truly correlates with CD8+TIL. Therefore, if you cannot perform
protein expression analysis such as immunostaining, please mention it in the limitation in the
discussion.
## Although these genes, EPS8L3, MUC13 and PLEKHS1, have been shown to be involved
with cancer cells, it remains unclear how they interact immunologically. Please add another
immunological discussion. If not, please state in the discussion that these mechanisms have not
been shown in the previous report.
I hope these comment will be helpful.
assistant