Title: The Effect of Trolox on the Rabbit Anal Sphincterotomy Repair
Version: 1 Date: 01 May 2023
Reviewer:  Massimo Mongardini
Reviewer's report:
Considerations
In the introduction and in the discussion, the statement that sphincter injury surgery produces
an insufficient result is not precise: the result of sphincter repairs is directly proportional to the
earliness of the repair itself: in obstetric injuries with immediate surgical repair, operated in
high-volume centres, the success rate even in the long time is optimal and favourable.
The role of vitamin E in antioxidant and antifibrotic processes is certainly important, but a
proactive role in muscle regeneration need to be strongly demonstrated: this is the underlying
reason, perhaps not clearly stressed in the workplace. There is no specification of the extent
(extension, not just length) of the damage and the times of injury/repair.
The method of detecting manometry and electromyography should be specified: were the rabbits
sedated for the examination, for how long and in what manner? How much does this sedation
affect the reported findings?
A further study group would have been useful in which Trolox was taken after the lesion and
not at the same time and before the lesion itself: this to evaluate the real clinical applicability of
the procedure: no patient with lesions knows before being injured and, in the best of hypothesis,
obstetric lesions with evident symptoms are repaired or repairable after a few days or weeks.
It would also be useful to verify the reliability of vitamin E after ablation of more significant
quantities of the sphincter system (5-10 degrees - 1 or 2 cm) to actually measure the myogenic
potential. The study track could be interesting by combining the demonstrated capabilities of
Trolox with early surgery. In this way the meaning of the study could be clearer and more
evident.
Title: The Effect of Trolox on the Rabbit Anal Sphincterotomy Repair
Version: 1 Date: 01 May 2023
Reviewer:  Massimo Mongardini
Reviewer's report:
Considerations
In the introduction and in the discussion, the statement that sphincter injury surgery produces
an insufficient result is not precise: the result of sphincter repairs is directly proportional to the
earliness of the repair itself: in obstetric injuries with immediate surgical repair, operated in
high-volume centres, the success rate even in the long time is optimal and favourable.
The role of vitamin E in antioxidant and antifibrotic processes is certainly important, but a
proactive role in muscle regeneration need to be strongly demonstrated: this is the underlying
reason, perhaps not clearly stressed in the workplace. There is no specification of the extent
(extension, not just length) of the damage and the times of injury/repair.
The method of detecting manometry and electromyography should be specified: were the rabbits
sedated for the examination, for how long and in what manner? How much does this sedation
affect the reported findings?
A further study group would have been useful in which Trolox was taken after the lesion and
not at the same time and before the lesion itself: this to evaluate the real clinical applicability of
the procedure: no patient with lesions knows before being injured and, in the best of hypothesis,
obstetric lesions with evident symptoms are repaired or repairable after a few days or weeks.
It would also be useful to verify the reliability of vitamin E after ablation of more significant
quantities of the sphincter system (5-10 degrees - 1 or 2 cm) to actually measure the myogenic
potential. The study track could be interesting by combining the demonstrated capabilities of
Trolox with early surgery. In this way the meaning of the study could be clearer and more
evident.
Please review this paper.
user
Title: The Effect of Trolox on the Rabbit Anal Sphincterotomy Repair
Version: 1 Date: 01 May 2023
Reviewer:  Massimo Mongardini
Reviewer's report:
Considerations
In the introduction and in the discussion, the statement that sphincter injury surgery produces
an insufficient result is not precise: the result of sphincter repairs is directly proportional to the
earliness of the repair itself: in obstetric injuries with immediate surgical repair, operated in
high-volume centres, the success rate even in the long time is optimal and favourable.
The role of vitamin E in antioxidant and antifibrotic processes is certainly important, but a
proactive role in muscle regeneration need to be strongly demonstrated: this is the underlying
reason, perhaps not clearly stressed in the workplace. There is no specification of the extent
(extension, not just length) of the damage and the times of injury/repair.
The method of detecting manometry and electromyography should be specified: were the rabbits
sedated for the examination, for how long and in what manner? How much does this sedation
affect the reported findings?
A further study group would have been useful in which Trolox was taken after the lesion and
not at the same time and before the lesion itself: this to evaluate the real clinical applicability of
the procedure: no patient with lesions knows before being injured and, in the best of hypothesis,
obstetric lesions with evident symptoms are repaired or repairable after a few days or weeks.
It would also be useful to verify the reliability of vitamin E after ablation of more significant
quantities of the sphincter system (5-10 degrees - 1 or 2 cm) to actually measure the myogenic
potential. The study track could be interesting by combining the demonstrated capabilities of
Trolox with early surgery. In this way the meaning of the study could be clearer and more
evident.
assistant