Title: Analysis of the NUDT15 Gene and Metabolites of Azathioprine in Japanese Patients with
Inflammatory Bowel Disease
Version: 0 Date: 24 May 2023
Reviewer:  Devendra Desai
Reviewer's report:
This study looks at the molecular causes for thiopurine toxicity, ITPA, NUDT 15 and TPMT.
In introduction the authors should remove reference to their previous study and add the aims.
Why were older blood sample used in all patients?  Was there any fixed time for thiopurine
metabolite measurement?  Can we compare the measurement made at week 8 with measurement
at week 52?
Authors should also explain why they decided to club the molecular analysis with thiopurine
metabolite measurement.
The result of this study suggests that leucopenia may be related to ITPA and /or NUDT
15 genotype variation. However some of the recent studies show that ITPA variation is not
important in causing leucopenia. So authors need to discuss it.
Finally there are some spelling mistakes that need correction.
Title: Analysis of the NUDT15 Gene and Metabolites of Azathioprine in Japanese Patients with
Inflammatory Bowel Disease
Version: 0 Date: 24 May 2023
Reviewer:  Devendra Desai
Reviewer's report:
This study looks at the molecular causes for thiopurine toxicity, ITPA, NUDT 15 and TPMT.
In introduction the authors should remove reference to their previous study and add the aims.
Why were older blood sample used in all patients?  Was there any fixed time for thiopurine
metabolite measurement?  Can we compare the measurement made at week 8 with measurement
at week 52?
Authors should also explain why they decided to club the molecular analysis with thiopurine
metabolite measurement.
The result of this study suggests that leucopenia may be related to ITPA and /or NUDT
15 genotype variation. However some of the recent studies show that ITPA variation is not
important in causing leucopenia. So authors need to discuss it.
Finally there are some spelling mistakes that need correction.
Please review this paper.
user
Title: Analysis of the NUDT15 Gene and Metabolites of Azathioprine in Japanese Patients with
Inflammatory Bowel Disease
Version: 0 Date: 24 May 2023
Reviewer:  Devendra Desai
Reviewer's report:
This study looks at the molecular causes for thiopurine toxicity, ITPA, NUDT 15 and TPMT.
In introduction the authors should remove reference to their previous study and add the aims.
Why were older blood sample used in all patients?  Was there any fixed time for thiopurine
metabolite measurement?  Can we compare the measurement made at week 8 with measurement
at week 52?
Authors should also explain why they decided to club the molecular analysis with thiopurine
metabolite measurement.
The result of this study suggests that leucopenia may be related to ITPA and /or NUDT
15 genotype variation. However some of the recent studies show that ITPA variation is not
important in causing leucopenia. So authors need to discuss it.
Finally there are some spelling mistakes that need correction.
assistant