Title: CDH1 genetic variants and its aberrant expression are the risk factors for colorectal cancer
metastasis
Version: 3 Date: 05 Jan 2025
Reviewer's report:
This study identified the association between the CDH1-160C>A SNP and colorectal cancer
(CRC) development and progression, exploring how the downregulation of E-cadherin
contributes to intestinal carcinogenesis and metastasis, with the aim of identifying potential
molecular mechanisms and early diagnostic biomarkers.
We recommend addressing following points for the article more informative.
First, the detailed mechanisms by which the CDH1-160 polymorphism influences E-cadherin
transcription and expression are not fully explored. Factors like transcription factor binding or
epigenetic modifications should be considered.
The study focuses solely on CDH1 but does not consider the roles of other key genes (e.g., APC,
KRAS, p53) in CRC progression and metastasis. Examining potential interactions with other
genetic factors would provide a more comprehensive understanding.
Finally, E-cadherin expression is evaluated using a semi-quantitative scale, which lacks
precision. Incorporating quantitative techniques such as digital image analysis or fluorescence
intensity measurements would enhance accuracy.
Title: CDH1 genetic variants and its aberrant expression are the risk factors for colorectal cancer
metastasis
Version: 3 Date: 05 Jan 2025
Reviewer's report:
This study identified the association between the CDH1-160C>A SNP and colorectal cancer
(CRC) development and progression, exploring how the downregulation of E-cadherin
contributes to intestinal carcinogenesis and metastasis, with the aim of identifying potential
molecular mechanisms and early diagnostic biomarkers.
We recommend addressing following points for the article more informative.
First, the detailed mechanisms by which the CDH1-160 polymorphism influences E-cadherin
transcription and expression are not fully explored. Factors like transcription factor binding or
epigenetic modifications should be considered.
The study focuses solely on CDH1 but does not consider the roles of other key genes (e.g., APC,
KRAS, p53) in CRC progression and metastasis. Examining potential interactions with other
genetic factors would provide a more comprehensive understanding.
Finally, E-cadherin expression is evaluated using a semi-quantitative scale, which lacks
precision. Incorporating quantitative techniques such as digital image analysis or fluorescence
intensity measurements would enhance accuracy.
Please review this paper.
user
Title: CDH1 genetic variants and its aberrant expression are the risk factors for colorectal cancer
metastasis
Version: 3 Date: 05 Jan 2025
Reviewer's report:
This study identified the association between the CDH1-160C>A SNP and colorectal cancer
(CRC) development and progression, exploring how the downregulation of E-cadherin
contributes to intestinal carcinogenesis and metastasis, with the aim of identifying potential
molecular mechanisms and early diagnostic biomarkers.
We recommend addressing following points for the article more informative.
First, the detailed mechanisms by which the CDH1-160 polymorphism influences E-cadherin
transcription and expression are not fully explored. Factors like transcription factor binding or
epigenetic modifications should be considered.
The study focuses solely on CDH1 but does not consider the roles of other key genes (e.g., APC,
KRAS, p53) in CRC progression and metastasis. Examining potential interactions with other
genetic factors would provide a more comprehensive understanding.
Finally, E-cadherin expression is evaluated using a semi-quantitative scale, which lacks
precision. Incorporating quantitative techniques such as digital image analysis or fluorescence
intensity measurements would enhance accuracy.
assistant