Title: Detection and characterization of human astrovirus and sapovirus in outpatients with acute
gastroenteritis in Guangzhou, China
Version: 1 Date: 17 Aug 2021
Reviewer's report:
This study determined the incidence of the infection caused by HAstV and SaV in people with
AGE inGuangzhou, China, from September 2013 to January 2016 and characterized the HAstV
and SaV detected according to the genotypes.The results of this study may provide further
epidemiological and molecular information about HAstV and SaV strains causing AGE. This
information may prove useful in the development of vaccines and other preventative therapies
for AGE and related diseases. Totally, the manuscript is well written, but several issues need
to be addressed:
1.Line 107, not “a phylogenetic tree” here, “a” is redundant;
2.Line 110, “The following reference stains and accession numbers were used”. Typo “stains”.
Please elaborate why chose these sequences as references? Did they exhibit different genotypes?
3.Line 134-135. “We also detected three cases of multiple gastrointestinal tract infections.”
Please describe in detail.
4.Line 146-149. What is the distribution of different ages? Please state in sentences or by using
a graph. Then using infection rates for age distribution in HAstV and SaV infections, not just
by case numbers.
5.Line 156-158. “ However, AGE with single HAstV infection is more likely to cause fever and
has been observed in five of the ten cases (50%) compared to infections caused by undetected
pathogens (16.81%)”. Why not use all the HAstV-positive cases here? It’s more reasonable to
use all the HAstV-positive cases here.
6.Line 200-202, “our data also showed that the median age of patients with the single HAstV
infection was higher than that of the patients with other virus infections (P = .0476).” Please
state the potential reason for the phenomenon here.
7.Line 202-204. Why “50% of those with HAstV infection also reported AGE” can “suggesting
that people of all age may be susceptible to HAstV, not just the very young and the elderly”?
Please explain.
8.Line 209-210. “we identified another peak of SaV infection from February to March (41.67%),
indicating the possibility of a high prevalence during early spring months too”, please state the
reason. Is it because of outbreak?
Title: Detection and characterization of human astrovirus and sapovirus in outpatients with acute
gastroenteritis in Guangzhou, China
Version: 1 Date: 17 Aug 2021
Reviewer's report:
This study determined the incidence of the infection caused by HAstV and SaV in people with
AGE inGuangzhou, China, from September 2013 to January 2016 and characterized the HAstV
and SaV detected according to the genotypes.The results of this study may provide further
epidemiological and molecular information about HAstV and SaV strains causing AGE. This
information may prove useful in the development of vaccines and other preventative therapies
for AGE and related diseases. Totally, the manuscript is well written, but several issues need
to be addressed:
1.Line 107, not “a phylogenetic tree” here, “a” is redundant;
2.Line 110, “The following reference stains and accession numbers were used”. Typo “stains”.
Please elaborate why chose these sequences as references? Did they exhibit different genotypes?
3.Line 134-135. “We also detected three cases of multiple gastrointestinal tract infections.”
Please describe in detail.
4.Line 146-149. What is the distribution of different ages? Please state in sentences or by using
a graph. Then using infection rates for age distribution in HAstV and SaV infections, not just
by case numbers.
5.Line 156-158. “ However, AGE with single HAstV infection is more likely to cause fever and
has been observed in five of the ten cases (50%) compared to infections caused by undetected
pathogens (16.81%)”. Why not use all the HAstV-positive cases here? It’s more reasonable to
use all the HAstV-positive cases here.
6.Line 200-202, “our data also showed that the median age of patients with the single HAstV
infection was higher than that of the patients with other virus infections (P = .0476).” Please
state the potential reason for the phenomenon here.
7.Line 202-204. Why “50% of those with HAstV infection also reported AGE” can “suggesting
that people of all age may be susceptible to HAstV, not just the very young and the elderly”?
Please explain.
8.Line 209-210. “we identified another peak of SaV infection from February to March (41.67%),
indicating the possibility of a high prevalence during early spring months too”, please state the
reason. Is it because of outbreak?
Please review this paper.
user
Title: Detection and characterization of human astrovirus and sapovirus in outpatients with acute
gastroenteritis in Guangzhou, China
Version: 1 Date: 17 Aug 2021
Reviewer's report:
This study determined the incidence of the infection caused by HAstV and SaV in people with
AGE inGuangzhou, China, from September 2013 to January 2016 and characterized the HAstV
and SaV detected according to the genotypes.The results of this study may provide further
epidemiological and molecular information about HAstV and SaV strains causing AGE. This
information may prove useful in the development of vaccines and other preventative therapies
for AGE and related diseases. Totally, the manuscript is well written, but several issues need
to be addressed:
1.Line 107, not “a phylogenetic tree” here, “a” is redundant;
2.Line 110, “The following reference stains and accession numbers were used”. Typo “stains”.
Please elaborate why chose these sequences as references? Did they exhibit different genotypes?
3.Line 134-135. “We also detected three cases of multiple gastrointestinal tract infections.”
Please describe in detail.
4.Line 146-149. What is the distribution of different ages? Please state in sentences or by using
a graph. Then using infection rates for age distribution in HAstV and SaV infections, not just
by case numbers.
5.Line 156-158. “ However, AGE with single HAstV infection is more likely to cause fever and
has been observed in five of the ten cases (50%) compared to infections caused by undetected
pathogens (16.81%)”. Why not use all the HAstV-positive cases here? It’s more reasonable to
use all the HAstV-positive cases here.
6.Line 200-202, “our data also showed that the median age of patients with the single HAstV
infection was higher than that of the patients with other virus infections (P = .0476).” Please
state the potential reason for the phenomenon here.
7.Line 202-204. Why “50% of those with HAstV infection also reported AGE” can “suggesting
that people of all age may be susceptible to HAstV, not just the very young and the elderly”?
Please explain.
8.Line 209-210. “we identified another peak of SaV infection from February to March (41.67%),
indicating the possibility of a high prevalence during early spring months too”, please state the
reason. Is it because of outbreak?
assistant