Reviewer's report
Title:Lipoprotein lipase activity and mass, apolipoprotein C-II mass and
polymorphisms of apolipoproteins E and A5 in subjects with prior acute
hypertriglyceridaemic pancreatitis
Version: 1Date:26 March 2009
Reviewer: John Brunzell
Reviewer's report:
Major:
1. The authors addressed whether biochemical or clinical differences could be
noted in severely hypertriglyceridemic patients who had and did not have
pancreatitis. Other than finding 5 patients with LPL deficiency, there were few
differences. A bordrline increase in apoA5 snp was noted in HTG with
pancreatitis. The first paragraph is not clear about this.
2. Results: Estimates of positive family history are probably underestimates
unless relatives were actually studied. This should be noted.
3. The measures of chylomicron lipids are estimates at best. This technique is
not able to distinguish between smaller chylomicrons and larger VLDL. Did they
measure apoB48 and apoB100? NMR has not been shown to differentiate
between these particles either. They should call their chylomicrons "estimated".
Minor:
1. Page 3, line 2: "1.3-3.5%" of what?
2. Page 4, line 6: Delete word "promising". This is still experimental.
3. Page 4, para 3: Change lipidaemic to lipaemic.
4. Page 6, para 3: Split last sentence with period after word "plasma". Start
second part of sentence with "One mU".
5. Results: Were fibrates started after the bouts of pancreatitis? This might lead
to further underestimation of triglyceride levels.
6. Put BMI into table, perhaps in place of weight.
7. Page 10, end of para 2: add "or by measuring postheparin plasma LPL
activity"
8. Page 11, delete last sentence in para 1. See major comment #3 above.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:Lipoprotein lipase activity and mass, apolipoprotein C-II mass and
polymorphisms of apolipoproteins E and A5 in subjects with prior acute
hypertriglyceridaemic pancreatitis
Version: 1Date:26 March 2009
Reviewer: John Brunzell
Reviewer's report:
Major:
1. The authors addressed whether biochemical or clinical differences could be
noted in severely hypertriglyceridemic patients who had and did not have
pancreatitis. Other than finding 5 patients with LPL deficiency, there were few
differences. A bordrline increase in apoA5 snp was noted in HTG with
pancreatitis. The first paragraph is not clear about this.
2. Results: Estimates of positive family history are probably underestimates
unless relatives were actually studied. This should be noted.
3. The measures of chylomicron lipids are estimates at best. This technique is
not able to distinguish between smaller chylomicrons and larger VLDL. Did they
measure apoB48 and apoB100? NMR has not been shown to differentiate
between these particles either. They should call their chylomicrons "estimated".
Minor:
1. Page 3, line 2: "1.3-3.5%" of what?
2. Page 4, line 6: Delete word "promising". This is still experimental.
3. Page 4, para 3: Change lipidaemic to lipaemic.
4. Page 6, para 3: Split last sentence with period after word "plasma". Start
second part of sentence with "One mU".
5. Results: Were fibrates started after the bouts of pancreatitis? This might lead
to further underestimation of triglyceride levels.
6. Put BMI into table, perhaps in place of weight.
7. Page 10, end of para 2: add "or by measuring postheparin plasma LPL
activity"
8. Page 11, delete last sentence in para 1. See major comment #3 above.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:Lipoprotein lipase activity and mass, apolipoprotein C-II mass and
polymorphisms of apolipoproteins E and A5 in subjects with prior acute
hypertriglyceridaemic pancreatitis
Version: 1Date:26 March 2009
Reviewer: John Brunzell
Reviewer's report:
Major:
1. The authors addressed whether biochemical or clinical differences could be
noted in severely hypertriglyceridemic patients who had and did not have
pancreatitis. Other than finding 5 patients with LPL deficiency, there were few
differences. A bordrline increase in apoA5 snp was noted in HTG with
pancreatitis. The first paragraph is not clear about this.
2. Results: Estimates of positive family history are probably underestimates
unless relatives were actually studied. This should be noted.
3. The measures of chylomicron lipids are estimates at best. This technique is
not able to distinguish between smaller chylomicrons and larger VLDL. Did they
measure apoB48 and apoB100? NMR has not been shown to differentiate
between these particles either. They should call their chylomicrons "estimated".
Minor:
1. Page 3, line 2: "1.3-3.5%" of what?
2. Page 4, line 6: Delete word "promising". This is still experimental.
3. Page 4, para 3: Change lipidaemic to lipaemic.
4. Page 6, para 3: Split last sentence with period after word "plasma". Start
second part of sentence with "One mU".
5. Results: Were fibrates started after the bouts of pancreatitis? This might lead
to further underestimation of triglyceride levels.
6. Put BMI into table, perhaps in place of weight.
7. Page 10, end of para 2: add "or by measuring postheparin plasma LPL
activity"
8. Page 11, delete last sentence in para 1. See major comment #3 above.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
assistant