Reviewer's report
Title:The balance between two isoforms of LEF-1 is a regulator in the growth of
colon carcinoma
Version: 4Date:8 February 2012
Reviewer: Andrew Quest
Reviewer's report:
As indicated previously, the authors addressed many of my previous comments
in a satisfactory fashion with the exception of those concerning Figures 2 and 6.
In this second revision these points were addressed again.
Previous review: The authors responded that they had quantified the flow
cytometry data and observed significant increases in G0/G1. However, my
question related to the SUB G0/G1 population that would be expected since it is
indicative of apoptosis. Accumulation in G0/G1 alone is more indicative of cell
cycle arrest.
The authors now provide a possible explanation that should be included in the
discussion.
With respect to Figure 6, I commented that the authors should evaluate whether
reduced tumor vascularization observed for cells expressing the LEF-1-dL variant
is also significantly reduced in tumors of the same size.
The authors did this analysis and found differences that were not statistically
signifcant. This is now stated in the manuscript. However, with this finding, the
consequence of changes in expression of Lef-1 variants in vivo remains unclear.
The title was changed but is not readily understandable as is.
The balance between two isoforms of LEF-1 is a regulator in the growth of colon
carcinoma
Should perhaps be changed to
The balance between two isoforms of LEF-1 regulates colon carcinoma growth
Sections added to this manuscript need to be revised by a native English
speaker
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
no competing interests
Reviewer's report
Title:The balance between two isoforms of LEF-1 is a regulator in the growth of
colon carcinoma
Version: 4Date:8 February 2012
Reviewer: Andrew Quest
Reviewer's report:
As indicated previously, the authors addressed many of my previous comments
in a satisfactory fashion with the exception of those concerning Figures 2 and 6.
In this second revision these points were addressed again.
Previous review: The authors responded that they had quantified the flow
cytometry data and observed significant increases in G0/G1. However, my
question related to the SUB G0/G1 population that would be expected since it is
indicative of apoptosis. Accumulation in G0/G1 alone is more indicative of cell
cycle arrest.
The authors now provide a possible explanation that should be included in the
discussion.
With respect to Figure 6, I commented that the authors should evaluate whether
reduced tumor vascularization observed for cells expressing the LEF-1-dL variant
is also significantly reduced in tumors of the same size.
The authors did this analysis and found differences that were not statistically
signifcant. This is now stated in the manuscript. However, with this finding, the
consequence of changes in expression of Lef-1 variants in vivo remains unclear.
The title was changed but is not readily understandable as is.
The balance between two isoforms of LEF-1 is a regulator in the growth of colon
carcinoma
Should perhaps be changed to
The balance between two isoforms of LEF-1 regulates colon carcinoma growth
Sections added to this manuscript need to be revised by a native English
speaker
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
no competing interests
Please review this paper.
user
Reviewer's report
Title:The balance between two isoforms of LEF-1 is a regulator in the growth of
colon carcinoma
Version: 4Date:8 February 2012
Reviewer: Andrew Quest
Reviewer's report:
As indicated previously, the authors addressed many of my previous comments
in a satisfactory fashion with the exception of those concerning Figures 2 and 6.
In this second revision these points were addressed again.
Previous review: The authors responded that they had quantified the flow
cytometry data and observed significant increases in G0/G1. However, my
question related to the SUB G0/G1 population that would be expected since it is
indicative of apoptosis. Accumulation in G0/G1 alone is more indicative of cell
cycle arrest.
The authors now provide a possible explanation that should be included in the
discussion.
With respect to Figure 6, I commented that the authors should evaluate whether
reduced tumor vascularization observed for cells expressing the LEF-1-dL variant
is also significantly reduced in tumors of the same size.
The authors did this analysis and found differences that were not statistically
signifcant. This is now stated in the manuscript. However, with this finding, the
consequence of changes in expression of Lef-1 variants in vivo remains unclear.
The title was changed but is not readily understandable as is.
The balance between two isoforms of LEF-1 is a regulator in the growth of colon
carcinoma
Should perhaps be changed to
The balance between two isoforms of LEF-1 regulates colon carcinoma growth
Sections added to this manuscript need to be revised by a native English
speaker
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
no competing interests
assistant