Reviewer's report
Title:Paneth cell metaplasia in newly diagnosed inflammatory bowel disease in
children
Version:2Date:24 December 2013
Reviewer: Chris Dekaney
Reviewer's report:
The goal of this manuscript was to describe the presence of Paneth cell
metaplasia in newly diagnosed inflammatory bowel disease in children. The data
clearly demonstrates an increase in Paneth cell numbers in most colonic regions
in children with diagnosed IBD. While it is important to have an understanding of
how to define Paneth cell metaplasia in children there are several
issues/questions that need to be addressed.
Major Compulsory Revisions
1. What was the authors’ hypothesis for this study?
2. The control biopsies were taken from patients who had IBD-like symptoms. Is
it possible that the presence of Paneth cells in these patients were abnormal due
to the gastrointestinal issues they were having? Is it possible to follow up with
those patients to determine if they ultimately were diagnosed with IBD?
3. How are Paneth cells defined (i.e. H&E, lysozyme)? This should be noted in
the methods.
4. The authors should define acute and chronic inflammation. How were they
scored? Please define architectural distortion. How was it scored?
5. The authors mention no correlation of the degree of acute or chronic
inflammation or architectural distortion with Paneth cell number. The data for
acute and chronic inflammation and architectural distortion should be included in
the manuscript.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:Paneth cell metaplasia in newly diagnosed inflammatory bowel disease in
children
Version:2Date:24 December 2013
Reviewer: Chris Dekaney
Reviewer's report:
The goal of this manuscript was to describe the presence of Paneth cell
metaplasia in newly diagnosed inflammatory bowel disease in children. The data
clearly demonstrates an increase in Paneth cell numbers in most colonic regions
in children with diagnosed IBD. While it is important to have an understanding of
how to define Paneth cell metaplasia in children there are several
issues/questions that need to be addressed.
Major Compulsory Revisions
1. What was the authors’ hypothesis for this study?
2. The control biopsies were taken from patients who had IBD-like symptoms. Is
it possible that the presence of Paneth cells in these patients were abnormal due
to the gastrointestinal issues they were having? Is it possible to follow up with
those patients to determine if they ultimately were diagnosed with IBD?
3. How are Paneth cells defined (i.e. H&E, lysozyme)? This should be noted in
the methods.
4. The authors should define acute and chronic inflammation. How were they
scored? Please define architectural distortion. How was it scored?
5. The authors mention no correlation of the degree of acute or chronic
inflammation or architectural distortion with Paneth cell number. The data for
acute and chronic inflammation and architectural distortion should be included in
the manuscript.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:Paneth cell metaplasia in newly diagnosed inflammatory bowel disease in
children
Version:2Date:24 December 2013
Reviewer: Chris Dekaney
Reviewer's report:
The goal of this manuscript was to describe the presence of Paneth cell
metaplasia in newly diagnosed inflammatory bowel disease in children. The data
clearly demonstrates an increase in Paneth cell numbers in most colonic regions
in children with diagnosed IBD. While it is important to have an understanding of
how to define Paneth cell metaplasia in children there are several
issues/questions that need to be addressed.
Major Compulsory Revisions
1. What was the authors’ hypothesis for this study?
2. The control biopsies were taken from patients who had IBD-like symptoms. Is
it possible that the presence of Paneth cells in these patients were abnormal due
to the gastrointestinal issues they were having? Is it possible to follow up with
those patients to determine if they ultimately were diagnosed with IBD?
3. How are Paneth cells defined (i.e. H&E, lysozyme)? This should be noted in
the methods.
4. The authors should define acute and chronic inflammation. How were they
scored? Please define architectural distortion. How was it scored?
5. The authors mention no correlation of the degree of acute or chronic
inflammation or architectural distortion with Paneth cell number. The data for
acute and chronic inflammation and architectural distortion should be included in
the manuscript.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests.
assistant