Title: SPINK4 promotes colorectal cancer cell proliferation and inhibits ferroptosis
Version: 2 Date: 23 Feb 2023
Reviewer's report:
1.In figure 1 I and J, it did not show which picture represnets normal tissues and which represents
the tumor tissues,  I think this may confuse the readers; moreover, the rulers are missing in
Figure 1 I and J, I think it should be added  .
2.It did not show p values between different groups in Figure 2 A, I think it should be added.
3.According to the author's study showed that SPINK4 expression was reduced in CRC tissues
compared with normal tissues, however, SPINK4 expression in four CRC cell lines is higher than
that in the human colon mucosal epithelial cell line NCM460, and overexpression of SPINK4
significantly facilitated the proliferation and metastasis of LoVo and HCT116 cells. I think this
is contradictionary, please explain it.
4. I think the language needs polishing.
Title: SPINK4 promotes colorectal cancer cell proliferation and inhibits ferroptosis
Version: 2 Date: 23 Feb 2023
Reviewer's report:
1.In figure 1 I and J, it did not show which picture represnets normal tissues and which represents
the tumor tissues,  I think this may confuse the readers; moreover, the rulers are missing in
Figure 1 I and J, I think it should be added  .
2.It did not show p values between different groups in Figure 2 A, I think it should be added.
3.According to the author's study showed that SPINK4 expression was reduced in CRC tissues
compared with normal tissues, however, SPINK4 expression in four CRC cell lines is higher than
that in the human colon mucosal epithelial cell line NCM460, and overexpression of SPINK4
significantly facilitated the proliferation and metastasis of LoVo and HCT116 cells. I think this
is contradictionary, please explain it.
4. I think the language needs polishing.
Please review this paper.
user
Title: SPINK4 promotes colorectal cancer cell proliferation and inhibits ferroptosis
Version: 2 Date: 23 Feb 2023
Reviewer's report:
1.In figure 1 I and J, it did not show which picture represnets normal tissues and which represents
the tumor tissues,  I think this may confuse the readers; moreover, the rulers are missing in
Figure 1 I and J, I think it should be added  .
2.It did not show p values between different groups in Figure 2 A, I think it should be added.
3.According to the author's study showed that SPINK4 expression was reduced in CRC tissues
compared with normal tissues, however, SPINK4 expression in four CRC cell lines is higher than
that in the human colon mucosal epithelial cell line NCM460, and overexpression of SPINK4
significantly facilitated the proliferation and metastasis of LoVo and HCT116 cells. I think this
is contradictionary, please explain it.
4. I think the language needs polishing.
assistant