Reviewer ’s report  
 
Title: A Hypothesis of Couplet Molecules and Couplet Cells in Gastric Function and an 
Association with Helicobacter pylori  
 
 
Version: 0 Date: 13 Sep 2015  
 
 
Reviewer: Elisabetta Goni  
 
 
Reviewer's report:  
 
 
The full paper could be interesting but the main criticisms are:  
 
1. In backround it is not specify which kind go gastrin isoforms (e.g. G -17 G -34 G -14) 
 
2. Material and methods are completely lacking  
 
3. References are few and it could be increased  
 
4. The described model should be supported by data and better described  
 
 
Are the methods appropriate and well described?  
If not, please specify what is required in your comments to the aut hors.  
 
No 
 
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
 
No 
 
Are the conclusions drawn adequately supported by the data shown?  
If not, please explain in your comments to the authors.  
 
No 
 
Are you able to assess any statistics in the manuscript or would you recommend an 
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects require further 
assessment in your comments to the editors.  
 
Not relevant to this manuscript  
 
Quality of written English  
Please indicate the quality of language in the manuscript:  
 
Acceptable  
 
Declaration of competing interests  
Please complete a declaration of competing interests, considering the following questions:  
 
1. Have you in the past five year s received reimbursements, fees, funding, or salary from 
an organisation that may in any way gain or lose financially from the publication of this  
manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares in an organisation that may in a ny way gain or lose  
financially from the publication of this manuscript, either now or in the future?  
 
3. Do you hold or are you currently applying for any patents relating to the content of the  
manuscript?  
 
4. Have you received reimbursements, fees, funding , or salary from an organization that 
holds  or has applied for patents relating to the content of the manuscript?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have any non -financial competing interests in relation to this paper?  
 
 
If you  can answer no to all of the above, write 'I declare that I have no competing interests' 
below.  If your reply is yes to any, please give details below.  
 
I declare that I have no competition interests  
 
I agree to the open peer review policy of the journal. I un derstand that my name will be included  
on my report to the authors and, if the manuscript is accepted for publication, my named report  
including any attachments I upload will be posted on the website along with the authors'  
responses. I agree for my report  to be made available under an Open Access Creative Commons  
CC-BY lice nse ( http://creativecommons.org/licenses/by/4.0/ ). I understand that any comments  
which I do not wish to be included in my named report can be included as confidential comments  
to the ed itors, which will not be published.  
 
I agree to the open peer review policy of the journal  
Reviewer ’s report  
 
Title: A Hypothesis of Couplet Molecules and Couplet Cells in Gastric Function and an 
Association with Helicobacter pylori  
 
 
Version: 0 Date: 13 Sep 2015  
 
 
Reviewer: Elisabetta Goni  
 
 
Reviewer's report:  
 
 
The full paper could be interesting but the main criticisms are:  
 
1. In backround it is not specify which kind go gastrin isoforms (e.g. G -17 G -34 G -14) 
 
2. Material and methods are completely lacking  
 
3. References are few and it could be increased  
 
4. The described model should be supported by data and better described  
 
 
Are the methods appropriate and well described?  
If not, please specify what is required in your comments to the aut hors.  
 
No 
 
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
 
No 
 
Are the conclusions drawn adequately supported by the data shown?  
If not, please explain in your comments to the authors.  
 
No 
 
Are you able to assess any statistics in the manuscript or would you recommend an 
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects require further 
assessment in your comments to the editors.  
 
Not relevant to this manuscript  
 
Quality of written English  
Please indicate the quality of language in the manuscript:  
 
Acceptable  
 
Declaration of competing interests  
Please complete a declaration of competing interests, considering the following questions:  
 
1. Have you in the past five year s received reimbursements, fees, funding, or salary from 
an organisation that may in any way gain or lose financially from the publication of this  
manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares in an organisation that may in a ny way gain or lose  
financially from the publication of this manuscript, either now or in the future?  
 
3. Do you hold or are you currently applying for any patents relating to the content of the  
manuscript?  
 
4. Have you received reimbursements, fees, funding , or salary from an organization that 
holds  or has applied for patents relating to the content of the manuscript?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have any non -financial competing interests in relation to this paper?  
 
 
If you  can answer no to all of the above, write 'I declare that I have no competing interests' 
below.  If your reply is yes to any, please give details below.  
 
I declare that I have no competition interests  
 
I agree to the open peer review policy of the journal. I un derstand that my name will be included  
on my report to the authors and, if the manuscript is accepted for publication, my named report  
including any attachments I upload will be posted on the website along with the authors'  
responses. I agree for my report  to be made available under an Open Access Creative Commons  
CC-BY lice nse ( http://creativecommons.org/licenses/by/4.0/ ). I understand that any comments  
which I do not wish to be included in my named report can be included as confidential comments  
to the ed itors, which will not be published.  
 
I agree to the open peer review policy of the journal  
Please review this paper.
user
Reviewer ’s report  
 
Title: A Hypothesis of Couplet Molecules and Couplet Cells in Gastric Function and an 
Association with Helicobacter pylori  
 
 
Version: 0 Date: 13 Sep 2015  
 
 
Reviewer: Elisabetta Goni  
 
 
Reviewer's report:  
 
 
The full paper could be interesting but the main criticisms are:  
 
1. In backround it is not specify which kind go gastrin isoforms (e.g. G -17 G -34 G -14) 
 
2. Material and methods are completely lacking  
 
3. References are few and it could be increased  
 
4. The described model should be supported by data and better described  
 
 
Are the methods appropriate and well described?  
If not, please specify what is required in your comments to the aut hors.  
 
No 
 
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
 
No 
 
Are the conclusions drawn adequately supported by the data shown?  
If not, please explain in your comments to the authors.  
 
No 
 
Are you able to assess any statistics in the manuscript or would you recommend an 
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects require further 
assessment in your comments to the editors.  
 
Not relevant to this manuscript  
 
Quality of written English  
Please indicate the quality of language in the manuscript:  
 
Acceptable  
 
Declaration of competing interests  
Please complete a declaration of competing interests, considering the following questions:  
 
1. Have you in the past five year s received reimbursements, fees, funding, or salary from 
an organisation that may in any way gain or lose financially from the publication of this  
manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares in an organisation that may in a ny way gain or lose  
financially from the publication of this manuscript, either now or in the future?  
 
3. Do you hold or are you currently applying for any patents relating to the content of the  
manuscript?  
 
4. Have you received reimbursements, fees, funding , or salary from an organization that 
holds  or has applied for patents relating to the content of the manuscript?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have any non -financial competing interests in relation to this paper?  
 
 
If you  can answer no to all of the above, write 'I declare that I have no competing interests' 
below.  If your reply is yes to any, please give details below.  
 
I declare that I have no competition interests  
 
I agree to the open peer review policy of the journal. I un derstand that my name will be included  
on my report to the authors and, if the manuscript is accepted for publication, my named report  
including any attachments I upload will be posted on the website along with the authors'  
responses. I agree for my report  to be made available under an Open Access Creative Commons  
CC-BY lice nse ( http://creativecommons.org/licenses/by/4.0/ ). I understand that any comments  
which I do not wish to be included in my named report can be included as confidential comments  
to the ed itors, which will not be published.  
 
I agree to the open peer review policy of the journal  
assistant