Title: The expression of interleukin-1# in patients with chronic hepatitis B treated with
Pegylated-interferon-# combined with tenofovir and monotherapy: analysis of 162 cases from
a single center
Version: 1 Date: 09 Mar 2023
Reviewer's report:
1.The language used in this article requires refinement.
2.The article needs to provide a clear explanation of the diagnostic criteria for patients with CHB.
3.Regarding the expression of IL-1# in Result 1, it is insufficient to rely solely on detecting
mRNA content. This is because an increase in mRNA level does not always translate to a
corresponding increase in protein level. Therefore, it is necessary to supplement with ELISA
assay to detect protein content in the cell supernatant.
4.To enhance the logical coherence of the article, the author should include an explanation of
the changes in HBV protein levels following treatment of HBV-infected liver cells with IL-1#,
in order to illustrate the potential antiviral activity of IL-1#.
Title: The expression of interleukin-1# in patients with chronic hepatitis B treated with
Pegylated-interferon-# combined with tenofovir and monotherapy: analysis of 162 cases from
a single center
Version: 1 Date: 09 Mar 2023
Reviewer's report:
1.The language used in this article requires refinement.
2.The article needs to provide a clear explanation of the diagnostic criteria for patients with CHB.
3.Regarding the expression of IL-1# in Result 1, it is insufficient to rely solely on detecting
mRNA content. This is because an increase in mRNA level does not always translate to a
corresponding increase in protein level. Therefore, it is necessary to supplement with ELISA
assay to detect protein content in the cell supernatant.
4.To enhance the logical coherence of the article, the author should include an explanation of
the changes in HBV protein levels following treatment of HBV-infected liver cells with IL-1#,
in order to illustrate the potential antiviral activity of IL-1#.
Please review this paper.
user
Title: The expression of interleukin-1# in patients with chronic hepatitis B treated with
Pegylated-interferon-# combined with tenofovir and monotherapy: analysis of 162 cases from
a single center
Version: 1 Date: 09 Mar 2023
Reviewer's report:
1.The language used in this article requires refinement.
2.The article needs to provide a clear explanation of the diagnostic criteria for patients with CHB.
3.Regarding the expression of IL-1# in Result 1, it is insufficient to rely solely on detecting
mRNA content. This is because an increase in mRNA level does not always translate to a
corresponding increase in protein level. Therefore, it is necessary to supplement with ELISA
assay to detect protein content in the cell supernatant.
4.To enhance the logical coherence of the article, the author should include an explanation of
the changes in HBV protein levels following treatment of HBV-infected liver cells with IL-1#,
in order to illustrate the potential antiviral activity of IL-1#.
assistant