Title: Development and Validation of Standard and Real Patient Gallstone Library Using
Fourier Transform Infra-Red Spectroscopy
Version: 0 Date: 29 Nov 2021
Reviewer's report:
1. In the Background section, explain why FT-IR can be used in the determination of Gallstones;
based on reference [9] further explain what molecules/components contained in a Gallstone
specimen can be detected by FT-IR. Add an example of other studies conducting FT-IR
studies for identification of disease or similar components/molecules with Gallstone specimens
(Cholesterol, Calcium , Bilirubin, Bile Salt).
2. In Method "Phase II: ...........Thermo Scientific™ QCheck™ algorithm, embedded within the
OMNIC™ software, was used to identify the unique spectral fingerprint of the patient samples
to match with known, standard material. Matching of >75% was taken as acceptable...." -->
please put reference(s) which said 75% is acceptable or why the value is taken.
3. For next research, a  PLSR calibration method can be developed for  rapid detection of
gallstones’ compositions  analysis.
Title: Development and Validation of Standard and Real Patient Gallstone Library Using
Fourier Transform Infra-Red Spectroscopy
Version: 0 Date: 29 Nov 2021
Reviewer's report:
1. In the Background section, explain why FT-IR can be used in the determination of Gallstones;
based on reference [9] further explain what molecules/components contained in a Gallstone
specimen can be detected by FT-IR. Add an example of other studies conducting FT-IR
studies for identification of disease or similar components/molecules with Gallstone specimens
(Cholesterol, Calcium , Bilirubin, Bile Salt).
2. In Method "Phase II: ...........Thermo Scientific™ QCheck™ algorithm, embedded within the
OMNIC™ software, was used to identify the unique spectral fingerprint of the patient samples
to match with known, standard material. Matching of >75% was taken as acceptable...." -->
please put reference(s) which said 75% is acceptable or why the value is taken.
3. For next research, a  PLSR calibration method can be developed for  rapid detection of
gallstones’ compositions  analysis.
Please review this paper.
user
Title: Development and Validation of Standard and Real Patient Gallstone Library Using
Fourier Transform Infra-Red Spectroscopy
Version: 0 Date: 29 Nov 2021
Reviewer's report:
1. In the Background section, explain why FT-IR can be used in the determination of Gallstones;
based on reference [9] further explain what molecules/components contained in a Gallstone
specimen can be detected by FT-IR. Add an example of other studies conducting FT-IR
studies for identification of disease or similar components/molecules with Gallstone specimens
(Cholesterol, Calcium , Bilirubin, Bile Salt).
2. In Method "Phase II: ...........Thermo Scientific™ QCheck™ algorithm, embedded within the
OMNIC™ software, was used to identify the unique spectral fingerprint of the patient samples
to match with known, standard material. Matching of >75% was taken as acceptable...." -->
please put reference(s) which said 75% is acceptable or why the value is taken.
3. For next research, a  PLSR calibration method can be developed for  rapid detection of
gallstones’ compositions  analysis.
assistant