Reviewer's report
Title:Lack of efficacy of blueberry in nutritional prevention of
azoxymethane-initiated cancers of rat small intestine and colon
Version: 1Date:15 May 2009
Reviewer: usanee Vinitketkumnuen
Reviewer's report:
General comments:
The manuscript reports the lack of preventive effects of blueberry fruits on
AOM-induced colon preneoplastic lesion (ACF) formation in rats. However, data
show that BB consumption may protect AOM-induced carcinogenesis in male
rats and male rats are more susceptible to AOM-induced ACF formation than
female rats. In addition, no data of food intake by each rat during experiment, the
lack of amount of bioactive component (anthocyanin) intake, makes the result
difficult to conclude.
- Major Compulsory Revisions
1. The authors should report the dose of bioactive compounds in BB that each rat
received. Why do female rats ‘ body weigh gain less than male rats? The author
should explain reason why use 10% BB instead of 5% from previous report,
because they found different and opposite results.
2. The authors have to define criteria the detection of AP (adenomatous polyps)
and AC (adenocarcinoma) in colon and small intestine.
3. The authors discuss about apoptosis mechanism in proximal part but no
experimental results.
- Minor Essential Revisions
1. The author should discuss why C-peptide in female rats is about 30% lower
than male rats.
Reviewer's report
Title:Lack of efficacy of blueberry in nutritional prevention of
azoxymethane-initiated cancers of rat small intestine and colon
Version: 1Date:15 May 2009
Reviewer: usanee Vinitketkumnuen
Reviewer's report:
General comments:
The manuscript reports the lack of preventive effects of blueberry fruits on
AOM-induced colon preneoplastic lesion (ACF) formation in rats. However, data
show that BB consumption may protect AOM-induced carcinogenesis in male
rats and male rats are more susceptible to AOM-induced ACF formation than
female rats. In addition, no data of food intake by each rat during experiment, the
lack of amount of bioactive component (anthocyanin) intake, makes the result
difficult to conclude.
- Major Compulsory Revisions
1. The authors should report the dose of bioactive compounds in BB that each rat
received. Why do female rats ‘ body weigh gain less than male rats? The author
should explain reason why use 10% BB instead of 5% from previous report,
because they found different and opposite results.
2. The authors have to define criteria the detection of AP (adenomatous polyps)
and AC (adenocarcinoma) in colon and small intestine.
3. The authors discuss about apoptosis mechanism in proximal part but no
experimental results.
- Minor Essential Revisions
1. The author should discuss why C-peptide in female rats is about 30% lower
than male rats.
Please review this paper.
user
Reviewer's report
Title:Lack of efficacy of blueberry in nutritional prevention of
azoxymethane-initiated cancers of rat small intestine and colon
Version: 1Date:15 May 2009
Reviewer: usanee Vinitketkumnuen
Reviewer's report:
General comments:
The manuscript reports the lack of preventive effects of blueberry fruits on
AOM-induced colon preneoplastic lesion (ACF) formation in rats. However, data
show that BB consumption may protect AOM-induced carcinogenesis in male
rats and male rats are more susceptible to AOM-induced ACF formation than
female rats. In addition, no data of food intake by each rat during experiment, the
lack of amount of bioactive component (anthocyanin) intake, makes the result
difficult to conclude.
- Major Compulsory Revisions
1. The authors should report the dose of bioactive compounds in BB that each rat
received. Why do female rats ‘ body weigh gain less than male rats? The author
should explain reason why use 10% BB instead of 5% from previous report,
because they found different and opposite results.
2. The authors have to define criteria the detection of AP (adenomatous polyps)
and AC (adenocarcinoma) in colon and small intestine.
3. The authors discuss about apoptosis mechanism in proximal part but no
experimental results.
- Minor Essential Revisions
1. The author should discuss why C-peptide in female rats is about 30% lower
than male rats.
assistant