Title: Variations in the gastric mucosal microbiome and its predictive functions between
chronic atrophic gastritis and chronic nonatrophic gastritis patients based on Helicobacter pylori
negativity (A study of variations in the gastric mucosal microbiome)
Version: 2 Date: 22 Mar 2025
Reviewer's report:
The authors have revised the manuscript, but I have not found  all of the corrections to be
satisfactory.
Ad. 1: The authors state that 'the analysis focused on identifying statistically significant
differences in microbial composition'. I am unclear whether they mean microbiome composition
or bacterial structure.
Ad. 3: The authors state that 'functional pathway predictions were performed using PICRUSt2
(Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) with the
KEGG database', but this has nothing to do with the evaluation of bacterial metabolism, e.g.
glucose uptake. Considering this, the statement 'We observed alterations in the metabolism and
signal transduction of gastric' should be removed.
Title: Variations in the gastric mucosal microbiome and its predictive functions between
chronic atrophic gastritis and chronic nonatrophic gastritis patients based on Helicobacter pylori
negativity (A study of variations in the gastric mucosal microbiome)
Version: 2 Date: 22 Mar 2025
Reviewer's report:
The authors have revised the manuscript, but I have not found  all of the corrections to be
satisfactory.
Ad. 1: The authors state that 'the analysis focused on identifying statistically significant
differences in microbial composition'. I am unclear whether they mean microbiome composition
or bacterial structure.
Ad. 3: The authors state that 'functional pathway predictions were performed using PICRUSt2
(Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) with the
KEGG database', but this has nothing to do with the evaluation of bacterial metabolism, e.g.
glucose uptake. Considering this, the statement 'We observed alterations in the metabolism and
signal transduction of gastric' should be removed.
Please review this paper.
user
Title: Variations in the gastric mucosal microbiome and its predictive functions between
chronic atrophic gastritis and chronic nonatrophic gastritis patients based on Helicobacter pylori
negativity (A study of variations in the gastric mucosal microbiome)
Version: 2 Date: 22 Mar 2025
Reviewer's report:
The authors have revised the manuscript, but I have not found  all of the corrections to be
satisfactory.
Ad. 1: The authors state that 'the analysis focused on identifying statistically significant
differences in microbial composition'. I am unclear whether they mean microbiome composition
or bacterial structure.
Ad. 3: The authors state that 'functional pathway predictions were performed using PICRUSt2
(Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) with the
KEGG database', but this has nothing to do with the evaluation of bacterial metabolism, e.g.
glucose uptake. Considering this, the statement 'We observed alterations in the metabolism and
signal transduction of gastric' should be removed.
assistant