Title: MiRNA-374b-5p and miRNA-106a-5p are related to inflammatory bowel disease via
regulating IL-10 and STAT3 signaling pathways
Version: 7 Date: 24 Aug 2022
Reviewer's report:
This is a very interesting manuscript. The authors investigated the role of miR-374-5p and
miR-160a-5p in regulating IBD development. The purpose of the article is original, and such
an important issue. The study is well designed. The introduction is objective and appropriate,
as well as the aims of the study. The methodological process was adequate and the results are
consistent.
I suggest a few reviews:
1. I suggest adding the number of Ethics Committee in the item “2. Materials and Methods” too,
even if that is already at the end of the manuscript.
2. I suggest including a table describing/contenting the RT-qPCR primer sequences, which are
in item “2.3 RT-qPCR”. I think that could be better for understanding.
3. It is important to add the code of antibodies used for Western Blotting analyses. In the item
“2.7 Western blotting”, the code of antibodies for “JAK1, IL-10” used is missing.
4. I also think that is important include the weak points of the study that were not mentioned.
Please do so, if you consider there is space for improvement.
Title: MiRNA-374b-5p and miRNA-106a-5p are related to inflammatory bowel disease via
regulating IL-10 and STAT3 signaling pathways
Version: 7 Date: 24 Aug 2022
Reviewer's report:
This is a very interesting manuscript. The authors investigated the role of miR-374-5p and
miR-160a-5p in regulating IBD development. The purpose of the article is original, and such
an important issue. The study is well designed. The introduction is objective and appropriate,
as well as the aims of the study. The methodological process was adequate and the results are
consistent.
I suggest a few reviews:
1. I suggest adding the number of Ethics Committee in the item “2. Materials and Methods” too,
even if that is already at the end of the manuscript.
2. I suggest including a table describing/contenting the RT-qPCR primer sequences, which are
in item “2.3 RT-qPCR”. I think that could be better for understanding.
3. It is important to add the code of antibodies used for Western Blotting analyses. In the item
“2.7 Western blotting”, the code of antibodies for “JAK1, IL-10” used is missing.
4. I also think that is important include the weak points of the study that were not mentioned.
Please do so, if you consider there is space for improvement.
Please review this paper.
user
Title: MiRNA-374b-5p and miRNA-106a-5p are related to inflammatory bowel disease via
regulating IL-10 and STAT3 signaling pathways
Version: 7 Date: 24 Aug 2022
Reviewer's report:
This is a very interesting manuscript. The authors investigated the role of miR-374-5p and
miR-160a-5p in regulating IBD development. The purpose of the article is original, and such
an important issue. The study is well designed. The introduction is objective and appropriate,
as well as the aims of the study. The methodological process was adequate and the results are
consistent.
I suggest a few reviews:
1. I suggest adding the number of Ethics Committee in the item “2. Materials and Methods” too,
even if that is already at the end of the manuscript.
2. I suggest including a table describing/contenting the RT-qPCR primer sequences, which are
in item “2.3 RT-qPCR”. I think that could be better for understanding.
3. It is important to add the code of antibodies used for Western Blotting analyses. In the item
“2.7 Western blotting”, the code of antibodies for “JAK1, IL-10” used is missing.
4. I also think that is important include the weak points of the study that were not mentioned.
Please do so, if you consider there is space for improvement.
assistant