Title: Dietary intervention reverses molecular markers of hepatocellular senescence in the GAN
diet-induced obese and biopsy-confirmed mouse model of NASH
Version: 1 Date: 10 Dec 2023
Reviewer's report:
Felnsted-Jensen M. et al, have nicely described how dietary intervention reverses molecular
signatures of hepatocellular senescence in GAN diet-induced mouse model of NASH. Studies in
mice models that resemble human pathophysiology are essential to improve the understanding
of human NASH and pave the way for novel therapeutic approaches.
In order to accept the article some minor issues need to be fixed.
First, figures should be reorganized since the section “Dietary intervention improves hallmarks
of NASH and fibrosis in GAN DIO-NASH mice” results confusing and adds complexity to
the reader due to constant jumps from one figure to another, without respecting the established
order. Figures and graphs should be reorganized in order to fit with the text.
Second, regarding liver histology, pictures of vehicle 8w and 16w are missing. They should be
included to have the full image.
Third, showing all the groups (Chow vehicle, DIO-NASH vehicles-8w,16w,24w- and DIO-
NASH Chow-reversal-8w,16,24w) in the RNA-seq results is key to understand the changes.
Statistical significances also need to be shown. Fig 5B, Fig 6C-D and Fig 8B need to me
modified. Additionally, a vast amount of genes has been studied and the reader could benefit
from some guidance to understand their relevance. In this regard, the most relevant results could
be confirmed by qPCR or WB.
Title: Dietary intervention reverses molecular markers of hepatocellular senescence in the GAN
diet-induced obese and biopsy-confirmed mouse model of NASH
Version: 1 Date: 10 Dec 2023
Reviewer's report:
Felnsted-Jensen M. et al, have nicely described how dietary intervention reverses molecular
signatures of hepatocellular senescence in GAN diet-induced mouse model of NASH. Studies in
mice models that resemble human pathophysiology are essential to improve the understanding
of human NASH and pave the way for novel therapeutic approaches.
In order to accept the article some minor issues need to be fixed.
First, figures should be reorganized since the section “Dietary intervention improves hallmarks
of NASH and fibrosis in GAN DIO-NASH mice” results confusing and adds complexity to
the reader due to constant jumps from one figure to another, without respecting the established
order. Figures and graphs should be reorganized in order to fit with the text.
Second, regarding liver histology, pictures of vehicle 8w and 16w are missing. They should be
included to have the full image.
Third, showing all the groups (Chow vehicle, DIO-NASH vehicles-8w,16w,24w- and DIO-
NASH Chow-reversal-8w,16,24w) in the RNA-seq results is key to understand the changes.
Statistical significances also need to be shown. Fig 5B, Fig 6C-D and Fig 8B need to me
modified. Additionally, a vast amount of genes has been studied and the reader could benefit
from some guidance to understand their relevance. In this regard, the most relevant results could
be confirmed by qPCR or WB.
Please review this paper.
user
Title: Dietary intervention reverses molecular markers of hepatocellular senescence in the GAN
diet-induced obese and biopsy-confirmed mouse model of NASH
Version: 1 Date: 10 Dec 2023
Reviewer's report:
Felnsted-Jensen M. et al, have nicely described how dietary intervention reverses molecular
signatures of hepatocellular senescence in GAN diet-induced mouse model of NASH. Studies in
mice models that resemble human pathophysiology are essential to improve the understanding
of human NASH and pave the way for novel therapeutic approaches.
In order to accept the article some minor issues need to be fixed.
First, figures should be reorganized since the section “Dietary intervention improves hallmarks
of NASH and fibrosis in GAN DIO-NASH mice” results confusing and adds complexity to
the reader due to constant jumps from one figure to another, without respecting the established
order. Figures and graphs should be reorganized in order to fit with the text.
Second, regarding liver histology, pictures of vehicle 8w and 16w are missing. They should be
included to have the full image.
Third, showing all the groups (Chow vehicle, DIO-NASH vehicles-8w,16w,24w- and DIO-
NASH Chow-reversal-8w,16,24w) in the RNA-seq results is key to understand the changes.
Statistical significances also need to be shown. Fig 5B, Fig 6C-D and Fig 8B need to me
modified. Additionally, a vast amount of genes has been studied and the reader could benefit
from some guidance to understand their relevance. In this regard, the most relevant results could
be confirmed by qPCR or WB.
assistant