Reviewer's report
Title:IQGAP1 and IQGAP2 are Reciprocally Altered in Hepatocellular
Carcinoma
Version: 2Date:24 September 2010
Reviewer: Jun-ichi Okano
Reviewer's report:
I have read carefully their revised article entitled “IQGAP1 and IQGAP2 are
Reciprocally Altered in Hepatocellular Carcinoma.” by White CD et al. They have
answered our questions in most parts. Therefore, I believe their revised article is
acceptable in BMC Gastroenterology except two minor points as below.
1. In page 5, lines 6 and 7, spaces are lost before units. “lysed in lysis buffer
(50mM Tris (pH 8.0), 100 mM NaF, 30 mM Na4P2O7, 2 mM Na2MoO4, 5 mM
EDTA, 2mM Na3VO4)” should be “lysed in lysis buffer (50 mM Tris (pH 8.0), 100
mM NaF, 30 mM Na4P2O7, 2 mM Na2MoO4, 5 mM EDTA, 2 mM Na3VO4)”.
2. In page 7, line 3, a space is necessary between “positive if more than 10% of
cells stained strongly in the cytoplasm.” and semi-title “Quantitative real-time
PCR”.
Best regards,
Jun-ichi Okano, M.D.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:IQGAP1 and IQGAP2 are Reciprocally Altered in Hepatocellular
Carcinoma
Version: 2Date:24 September 2010
Reviewer: Jun-ichi Okano
Reviewer's report:
I have read carefully their revised article entitled “IQGAP1 and IQGAP2 are
Reciprocally Altered in Hepatocellular Carcinoma.” by White CD et al. They have
answered our questions in most parts. Therefore, I believe their revised article is
acceptable in BMC Gastroenterology except two minor points as below.
1. In page 5, lines 6 and 7, spaces are lost before units. “lysed in lysis buffer
(50mM Tris (pH 8.0), 100 mM NaF, 30 mM Na4P2O7, 2 mM Na2MoO4, 5 mM
EDTA, 2mM Na3VO4)” should be “lysed in lysis buffer (50 mM Tris (pH 8.0), 100
mM NaF, 30 mM Na4P2O7, 2 mM Na2MoO4, 5 mM EDTA, 2 mM Na3VO4)”.
2. In page 7, line 3, a space is necessary between “positive if more than 10% of
cells stained strongly in the cytoplasm.” and semi-title “Quantitative real-time
PCR”.
Best regards,
Jun-ichi Okano, M.D.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:IQGAP1 and IQGAP2 are Reciprocally Altered in Hepatocellular
Carcinoma
Version: 2Date:24 September 2010
Reviewer: Jun-ichi Okano
Reviewer's report:
I have read carefully their revised article entitled “IQGAP1 and IQGAP2 are
Reciprocally Altered in Hepatocellular Carcinoma.” by White CD et al. They have
answered our questions in most parts. Therefore, I believe their revised article is
acceptable in BMC Gastroenterology except two minor points as below.
1. In page 5, lines 6 and 7, spaces are lost before units. “lysed in lysis buffer
(50mM Tris (pH 8.0), 100 mM NaF, 30 mM Na4P2O7, 2 mM Na2MoO4, 5 mM
EDTA, 2mM Na3VO4)” should be “lysed in lysis buffer (50 mM Tris (pH 8.0), 100
mM NaF, 30 mM Na4P2O7, 2 mM Na2MoO4, 5 mM EDTA, 2 mM Na3VO4)”.
2. In page 7, line 3, a space is necessary between “positive if more than 10% of
cells stained strongly in the cytoplasm.” and semi-title “Quantitative real-time
PCR”.
Best regards,
Jun-ichi Okano, M.D.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
assistant