Title: Sodium nitroprusside protects HFD induced gut dysfunction via activating AMPK#/
SIRT1 signaling
Version: 2 Date: 19 Jan 2021
Reviewer's report:
1. The methods used in this manuscript should note the reference.
2. How many animals for experiment? What's the house condition?
3. The language of this manuscript need to be improved.
4. why the expression of ZO-1 protein with the treatment of SNP is much higher than blank
control?
5. Only body weight changed, fat mass and fasting blood glucose content were measure, which
is not enough for evaluation obesity.
6.  The gut microbiota analysis was not enough,such as the richness (ACE and Chao1 indexes)
and diversity (Shannon and Simpson indexes) of microbial communities.
7. The relationship between the AMPK pathway and the intestinal barrier was not shown in the
manuscript.
8.The influence of NO on the AMPK pathway and tight junction proteins should be discussed
fully.
9. Each aspect of the study is relatively simple and needs to be further studied.
Title: Sodium nitroprusside protects HFD induced gut dysfunction via activating AMPK#/
SIRT1 signaling
Version: 2 Date: 19 Jan 2021
Reviewer's report:
1. The methods used in this manuscript should note the reference.
2. How many animals for experiment? What's the house condition?
3. The language of this manuscript need to be improved.
4. why the expression of ZO-1 protein with the treatment of SNP is much higher than blank
control?
5. Only body weight changed, fat mass and fasting blood glucose content were measure, which
is not enough for evaluation obesity.
6.  The gut microbiota analysis was not enough,such as the richness (ACE and Chao1 indexes)
and diversity (Shannon and Simpson indexes) of microbial communities.
7. The relationship between the AMPK pathway and the intestinal barrier was not shown in the
manuscript.
8.The influence of NO on the AMPK pathway and tight junction proteins should be discussed
fully.
9. Each aspect of the study is relatively simple and needs to be further studied.
Please review this paper.
user
Title: Sodium nitroprusside protects HFD induced gut dysfunction via activating AMPK#/
SIRT1 signaling
Version: 2 Date: 19 Jan 2021
Reviewer's report:
1. The methods used in this manuscript should note the reference.
2. How many animals for experiment? What's the house condition?
3. The language of this manuscript need to be improved.
4. why the expression of ZO-1 protein with the treatment of SNP is much higher than blank
control?
5. Only body weight changed, fat mass and fasting blood glucose content were measure, which
is not enough for evaluation obesity.
6.  The gut microbiota analysis was not enough,such as the richness (ACE and Chao1 indexes)
and diversity (Shannon and Simpson indexes) of microbial communities.
7. The relationship between the AMPK pathway and the intestinal barrier was not shown in the
manuscript.
8.The influence of NO on the AMPK pathway and tight junction proteins should be discussed
fully.
9. Each aspect of the study is relatively simple and needs to be further studied.
assistant