Reviewer's report
Title:Gene expression analysis of a Helicobacter pylori-infected and high-salt
diet-treated mouse gastric tumor model: Identification of CD177 as a novel
prognostic factor in patients with gastric cancer
Version: 1Date:1 October 2012
Reviewer: Matteo Fassan
Reviewer's report:
Gastric cancer still remains one of the most prominent cause of cancer-related
mortality worldwide. Helicobacter pylori (Hp) is considered the main etiological
determinant of both gastric precancerous lesions and intestinal-type (so called
epidemic) gastric adenocarcinoma. In this study, Dr. Toyoda and colleagues
identified CD177 as a novel biomarker in a HP-based murine model of gastric
adenocarcinoma. The Authors used appropriated methods of analysis that
yielded some interesting results. However, I have some concerns that the
authors should address:
• References should be up-dated and commented within the "Conclusions"
section. Some examples: 1) Gonda TA et al. (Folic Acid Increases Global DNA
Methylation and Reduces Inflammation to Prevent Helicobacter-Associated
Gastric Cancer in Mice. Gastroenterology 2012;142: 681-683) found CD177 as
dysregulated in a similar murine model; 2) Suh YS, et al. (The combined
expression of metaplasia biomarkers predicts the prognosis of gastric cancer.
Ann Surg Oncol. 2012;19:1240-9) for muc13 and gastric cancer.
• The three selected markers (CD177, muc17 and reg3g) should be tested by
qRT-PCR/IHC also in the adenomatous lesions to test if they are early
dysregulated in the carcinogenetic cascade.
• Instead of pooling the different RNAs (and for groups with different sample
sizes), it would be better to have analyzed (at least) three samples per group.
Please, add some comments and add an image of the microarray findings.
• 606 weeks is an unusual follow-up interval for advanced gastric
adenocarcinoma patients. Please, add median values.
• English should be improved.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Gene expression analysis of a Helicobacter pylori-infected and high-salt
diet-treated mouse gastric tumor model: Identification of CD177 as a novel
prognostic factor in patients with gastric cancer
Version: 1Date:1 October 2012
Reviewer: Matteo Fassan
Reviewer's report:
Gastric cancer still remains one of the most prominent cause of cancer-related
mortality worldwide. Helicobacter pylori (Hp) is considered the main etiological
determinant of both gastric precancerous lesions and intestinal-type (so called
epidemic) gastric adenocarcinoma. In this study, Dr. Toyoda and colleagues
identified CD177 as a novel biomarker in a HP-based murine model of gastric
adenocarcinoma. The Authors used appropriated methods of analysis that
yielded some interesting results. However, I have some concerns that the
authors should address:
• References should be up-dated and commented within the "Conclusions"
section. Some examples: 1) Gonda TA et al. (Folic Acid Increases Global DNA
Methylation and Reduces Inflammation to Prevent Helicobacter-Associated
Gastric Cancer in Mice. Gastroenterology 2012;142: 681-683) found CD177 as
dysregulated in a similar murine model; 2) Suh YS, et al. (The combined
expression of metaplasia biomarkers predicts the prognosis of gastric cancer.
Ann Surg Oncol. 2012;19:1240-9) for muc13 and gastric cancer.
• The three selected markers (CD177, muc17 and reg3g) should be tested by
qRT-PCR/IHC also in the adenomatous lesions to test if they are early
dysregulated in the carcinogenetic cascade.
• Instead of pooling the different RNAs (and for groups with different sample
sizes), it would be better to have analyzed (at least) three samples per group.
Please, add some comments and add an image of the microarray findings.
• 606 weeks is an unusual follow-up interval for advanced gastric
adenocarcinoma patients. Please, add median values.
• English should be improved.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Gene expression analysis of a Helicobacter pylori-infected and high-salt
diet-treated mouse gastric tumor model: Identification of CD177 as a novel
prognostic factor in patients with gastric cancer
Version: 1Date:1 October 2012
Reviewer: Matteo Fassan
Reviewer's report:
Gastric cancer still remains one of the most prominent cause of cancer-related
mortality worldwide. Helicobacter pylori (Hp) is considered the main etiological
determinant of both gastric precancerous lesions and intestinal-type (so called
epidemic) gastric adenocarcinoma. In this study, Dr. Toyoda and colleagues
identified CD177 as a novel biomarker in a HP-based murine model of gastric
adenocarcinoma. The Authors used appropriated methods of analysis that
yielded some interesting results. However, I have some concerns that the
authors should address:
• References should be up-dated and commented within the "Conclusions"
section. Some examples: 1) Gonda TA et al. (Folic Acid Increases Global DNA
Methylation and Reduces Inflammation to Prevent Helicobacter-Associated
Gastric Cancer in Mice. Gastroenterology 2012;142: 681-683) found CD177 as
dysregulated in a similar murine model; 2) Suh YS, et al. (The combined
expression of metaplasia biomarkers predicts the prognosis of gastric cancer.
Ann Surg Oncol. 2012;19:1240-9) for muc13 and gastric cancer.
• The three selected markers (CD177, muc17 and reg3g) should be tested by
qRT-PCR/IHC also in the adenomatous lesions to test if they are early
dysregulated in the carcinogenetic cascade.
• Instead of pooling the different RNAs (and for groups with different sample
sizes), it would be better to have analyzed (at least) three samples per group.
Please, add some comments and add an image of the microarray findings.
• 606 weeks is an unusual follow-up interval for advanced gastric
adenocarcinoma patients. Please, add median values.
• English should be improved.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
assistant