Reviewer's report
Title:Protective effects of hydrogen enriched saline on liver ischemia reperfusion
injury by reducing oxidative stress and HMGB1 release
Version: 1Date:20 May 2013
Reviewer: Min Fang
Reviewer's report:
Increasing evidence suggests that the hydrogen treatment is associated with
anti-inflammation, including myocardial IRI. However, the precise effect and
mechanism of hydrogen-enriched saline treatment in liver injury is still unclear. In
this report, the authors suggest that hydrogen-enriched saline treatment plays a
protective role in liver IRI setting. The result is interesting. However, I have some
concerns:
The manuscript just described the application of the hydrogen-enriched saline
attenuates the liver ischemia reperfusion injury, this is a phenomenon, the
authors should investigate the relevant mechanisms in depth. In addition,
previous other studies already reported the hydrogen-enriched saline has
anti-inflammation such as in the settings of sepsis and myocardial IRI, so the
novelty is limited.
Major points
1. The manuscript has mentioned hydrogen has anti-oxidative effects. However,
the study has no relevant data. Reactive oxygen species (ROS) must be
measured in the liver IRI setting.
2. NF-#B is a crucial nuclear factor controlling the inflammatory cytokines. The
hydrogen-enriched saline influencing the NF-#B signaling must be investigated.
Minor Points
1. In figure 1 legend, n=96? Does repeat the experiments 96 times?
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
no
Reviewer's report
Title:Protective effects of hydrogen enriched saline on liver ischemia reperfusion
injury by reducing oxidative stress and HMGB1 release
Version: 1Date:20 May 2013
Reviewer: Min Fang
Reviewer's report:
Increasing evidence suggests that the hydrogen treatment is associated with
anti-inflammation, including myocardial IRI. However, the precise effect and
mechanism of hydrogen-enriched saline treatment in liver injury is still unclear. In
this report, the authors suggest that hydrogen-enriched saline treatment plays a
protective role in liver IRI setting. The result is interesting. However, I have some
concerns:
The manuscript just described the application of the hydrogen-enriched saline
attenuates the liver ischemia reperfusion injury, this is a phenomenon, the
authors should investigate the relevant mechanisms in depth. In addition,
previous other studies already reported the hydrogen-enriched saline has
anti-inflammation such as in the settings of sepsis and myocardial IRI, so the
novelty is limited.
Major points
1. The manuscript has mentioned hydrogen has anti-oxidative effects. However,
the study has no relevant data. Reactive oxygen species (ROS) must be
measured in the liver IRI setting.
2. NF-#B is a crucial nuclear factor controlling the inflammatory cytokines. The
hydrogen-enriched saline influencing the NF-#B signaling must be investigated.
Minor Points
1. In figure 1 legend, n=96? Does repeat the experiments 96 times?
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
no
Please review this paper.
user
Reviewer's report
Title:Protective effects of hydrogen enriched saline on liver ischemia reperfusion
injury by reducing oxidative stress and HMGB1 release
Version: 1Date:20 May 2013
Reviewer: Min Fang
Reviewer's report:
Increasing evidence suggests that the hydrogen treatment is associated with
anti-inflammation, including myocardial IRI. However, the precise effect and
mechanism of hydrogen-enriched saline treatment in liver injury is still unclear. In
this report, the authors suggest that hydrogen-enriched saline treatment plays a
protective role in liver IRI setting. The result is interesting. However, I have some
concerns:
The manuscript just described the application of the hydrogen-enriched saline
attenuates the liver ischemia reperfusion injury, this is a phenomenon, the
authors should investigate the relevant mechanisms in depth. In addition,
previous other studies already reported the hydrogen-enriched saline has
anti-inflammation such as in the settings of sepsis and myocardial IRI, so the
novelty is limited.
Major points
1. The manuscript has mentioned hydrogen has anti-oxidative effects. However,
the study has no relevant data. Reactive oxygen species (ROS) must be
measured in the liver IRI setting.
2. NF-#B is a crucial nuclear factor controlling the inflammatory cytokines. The
hydrogen-enriched saline influencing the NF-#B signaling must be investigated.
Minor Points
1. In figure 1 legend, n=96? Does repeat the experiments 96 times?
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
no
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