Reviewer’s report
Title: 
Left-Side Incarcerated Amyand’s Hernia with Appendix and Caecum
provoke by Early Banana Diet: A Case Report
Version: 
1 
Date: 
11 Mar 2021
Reviewer's report:
PEER REVIEWER ASSESSMENTS: 
RELEVANCE - Does this case report make a contribution to medical
knowledge, have educational value, or highlight the need for a change in clinical
practice or diagnostic/prognostic approaches? 
Yes, this report contributes to medical knowledge
CASE DESCRIPTION - Are the details of the case sufficiently well described to
understand the patient's symptoms and course of treatment? 
Yes, the description of the case is sufficient
DIAGNOSIS/INTERPRETATION - Based on the facts presented, are the
diagnosis, interpretation, and course of treatment medically sound?
Yes, the work described is medically sound
DISCUSSION OF THE CASE - Does the discussion appropriately analyse the
importance of the findings and their relevance to future understanding of disease
processes, diagnosis or treatment? Has an adequate literature review pertinent to
the case been included?
No - there are minor issues
OVERALL MANUSCRIPT POTENTIAL - Could an appropriately REVISED
version of this work represent a technically sound contribution?
Probably - with minor revisions
PEER REVIEWER COMMENTS: 
GENERAL COMMENTS: What is your overall impression of the report? 
Note I was not the original reviewer therefore I will review this as a new
submission This submission needs quite a bit of editing for grammar and word
usage BUT it does have a potential tidally important message that an early
banana diet, especially in young children with an inguinal hernia can lead to
bowel incarceration . Also Amyand's hernia usually refers to just the appendix
being trapped in the hernia sac not the cecum as well 
I think this is a minor point
but should be acknowledged
Are you confident in the authors' description and interpretation of the report? 
The description is appropriate but the terminology of a true Amyand's hernia that
differentiates it from a more classic incarcerated inguinal hernia is that it usually
refers to just the appendix being incarcerated in the hernia sac and not the distal
small bowel and cecum. The title though is not accurate the patient did not have
appendicitis at least when I look at the appendix it appears normal
Have the authors clearly outlined the need for publication by novelty of the case
or the specific adverse event? 
Yes I think there is a definite message here for mothers in Indonesia. And for this
reason justifies strong consideration of publication especially is the manuscript
also describes how to educate the population of the importance of not using a
banana diet in neonates !
REQUESTED REVISIONS: 
Suggested changes 
1.please in the future number the pages OR number the lines in a progressive
way-not just a new set of 
numbers for each page ( unless you also number the
pages- this will make it much easier for the reviewer and the editor to address
suggestions to 
individual parts of the manuscript.
2 abstract line 6 
the incidence of 4 to 6% is far too high. Do you mean for
incarcerated hernias! 
Also you can say that this occurs primarily on the right side
in the first sentence and thereby delete the 5th sentence of the abstract. Also
most people think of Amyand's hernia as containing only the appendix
3. 4th sentence of abstract if there is small bowel obstruction( also note small
intestinal obstruction is usually referred to as "small bowel obstruction" and is
abbreviated SBO 
and not SIO) 
then abdominal distention 
can occur please
reword it also 
4. Abstract lines 30 and 31. What is a "cito 
herniotomy procedure"? 
Being a left
sided Amyand's hernia did the patient have situs inversus or 
was there an
extremely mobile cecum?
5. Abstract line 40 I would strongly suggest that this be reworded as " This case
suggests the possibility of early feeding of a banana diet may provoke
incarceration of an inguinal hernia and if the incarcerated hernia contents
contains the appendix, then an Amyand's hernia". 
5 Background page line 8 change in to "into" and reword the next sentence to
"Rarely, the appendix can be found in the inguinal sac of an inguinal hernia
which has been called an Amyand's hernia.
6. Background 
Again line 15. 4 to 6 % is far too high especially with the 1%
described in line 20 this needs to be changed to a more realistic %. 
7. Background line 25 and discussion line 52 change " followed by" to
"associated with" 
and change the word "diver" on line 30 to " differentiate" 
8. Background line 37 change "intestinal obstruction (IBO) " to "small bowel
obstruction( SBI)" this is the more common terminology in the literature
9. Background lines 47 and 50 do you mean "was" or "is"? 
That is, is the banana
diet still common today?
10. Case presentation line 6 and 8 change the word "repositioned" to " reduced"
also line 8 change the word " for" to " from"
11. Case presentation line 33 what do your mean by "excoriated " when
describing the cecum. The figure to me looks like an erythematous cecum not
excoriated; excoriation 
means external trauma with bleeding g from the serosal
surface
12. Case presentation line 38-40. You say " hernia repair" what do you mean by
this. Do you mean just ligation of the sac or did you really try to obliterate the
internal hernia defect ( internal ring- 
that would be quite unusual in a neonate!
13. Discussion line 15 change " could perform 
following" to " can 
develop in an
incarcerated" 
14 discussion lines 23-32. Please define all the types of Amyland's hernia so the
reader understands all the types
15. Discussion lines 35-47 did your patient have situs Inversus ? He obviously
had a mobile cecum you should describe or at the least say it. 
16 discussion is the early banana diet still common in Indonesia today? 
If so this
needs to be stressed and some statement made to highlight the risk of this
especially if the paper by Wiryo and colleagues really did show that the
incidence of SBO was as high as 9%; was this really good epidemiologic study?.
Also more solid foods do not stay as solid food in the small intestine e so the
ability of neonate to "digest" banana implies that there is a large amount of
nondigestable fiber in banana. This needs to be explained.
17. Your title needs to be changed. Your patient did not have appendicitis so
delete the term appendicitis in the title
18 on the conclusions-please make more effort to describe how to educate the
mothers to prevent this complication. Also change the term "morbidity " to "
intestinal obstruction". the term 
morbidity 
is too general.
ADDITIONAL REQUESTS/SUGGESTIONS: 
see above
Publons Reviewer Recognition
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review directly to Publons (a subsidiary of Clarivate Analytics). If you would
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Please complete a declaration of competing
interests, considering the following questions:
1
. 
Have you in the past five years received reimbursements, fees, funding, or
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from the publication of this manuscript, either now or in the future?
2
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3
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Have you received reimbursements, fees, funding, or salary from an
organization that holds or has applied for patents relating to the content of
the manuscript?
5
. 
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6
. 
Do you have any non-financial competing interests in relation to this
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If you can answer no to all of the above, write 'I declare that I have no competing
interests' below. If your reply is yes to any, please give details below.
This reviewer has been recruited by a partner organization, Research Square.
Reviewers with declared or apparent competing interests are not utilized for
these reviews. This reviewer has agreed to publication of their comments online
under a Creative Commons Attribution License attributed to Research Square
and was paid a small honorarium for completing the review within a specified
timeframe. Honoraria for reviews such as this are paid regardless of the reviewer
recommendation.
Reviewer Publication Consent.
 I agree for my report to be made available
under an Open Access Creative Commons CC-BY License (
http://creativecommons.org/licenses/by/4.0
) if this manuscript is accepted for
publication. Any comments that I do not wish to be included in the published
report have been included as confidential comments to the editor, which will not
be published.
If you are not happy for us to publish this report, please contact the
editorial office before completing the review.
If you wish, you can include your
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your name at the start of the process and confirm this decision whenever
prompted. If you change your decision later, we will not be able to publish your
name.
I agree to the terms of the CC-BY 4.0 license; please 
do not
 publish my name
with my report. (default)
Is the study design appropriate to answer the research question (including the use
of appropriate controls), and are the conclusions supported by the evidence
presented?
Yes
Are the methods sufficiently described to allow the study to be repeated?
Yes
Is the use of statistics and treatment of uncertainties appropriate?
Yes
Is the presentation of the work clear?
No
Are the images in this manuscript (including electrophoretic gels and blots) free
from apparent manipulation?
Yes
Reviewer’s report
Title: 
Left-Side Incarcerated Amyand’s Hernia with Appendix and Caecum
provoke by Early Banana Diet: A Case Report
Version: 
1 
Date: 
11 Mar 2021
Reviewer's report:
PEER REVIEWER ASSESSMENTS: 
RELEVANCE - Does this case report make a contribution to medical
knowledge, have educational value, or highlight the need for a change in clinical
practice or diagnostic/prognostic approaches? 
Yes, this report contributes to medical knowledge
CASE DESCRIPTION - Are the details of the case sufficiently well described to
understand the patient's symptoms and course of treatment? 
Yes, the description of the case is sufficient
DIAGNOSIS/INTERPRETATION - Based on the facts presented, are the
diagnosis, interpretation, and course of treatment medically sound?
Yes, the work described is medically sound
DISCUSSION OF THE CASE - Does the discussion appropriately analyse the
importance of the findings and their relevance to future understanding of disease
processes, diagnosis or treatment? Has an adequate literature review pertinent to
the case been included?
No - there are minor issues
OVERALL MANUSCRIPT POTENTIAL - Could an appropriately REVISED
version of this work represent a technically sound contribution?
Probably - with minor revisions
PEER REVIEWER COMMENTS: 
GENERAL COMMENTS: What is your overall impression of the report? 
Note I was not the original reviewer therefore I will review this as a new
submission This submission needs quite a bit of editing for grammar and word
usage BUT it does have a potential tidally important message that an early
banana diet, especially in young children with an inguinal hernia can lead to
bowel incarceration . Also Amyand's hernia usually refers to just the appendix
being trapped in the hernia sac not the cecum as well 
I think this is a minor point
but should be acknowledged
Are you confident in the authors' description and interpretation of the report? 
The description is appropriate but the terminology of a true Amyand's hernia that
differentiates it from a more classic incarcerated inguinal hernia is that it usually
refers to just the appendix being incarcerated in the hernia sac and not the distal
small bowel and cecum. The title though is not accurate the patient did not have
appendicitis at least when I look at the appendix it appears normal
Have the authors clearly outlined the need for publication by novelty of the case
or the specific adverse event? 
Yes I think there is a definite message here for mothers in Indonesia. And for this
reason justifies strong consideration of publication especially is the manuscript
also describes how to educate the population of the importance of not using a
banana diet in neonates !
REQUESTED REVISIONS: 
Suggested changes 
1.please in the future number the pages OR number the lines in a progressive
way-not just a new set of 
numbers for each page ( unless you also number the
pages- this will make it much easier for the reviewer and the editor to address
suggestions to 
individual parts of the manuscript.
2 abstract line 6 
the incidence of 4 to 6% is far too high. Do you mean for
incarcerated hernias! 
Also you can say that this occurs primarily on the right side
in the first sentence and thereby delete the 5th sentence of the abstract. Also
most people think of Amyand's hernia as containing only the appendix
3. 4th sentence of abstract if there is small bowel obstruction( also note small
intestinal obstruction is usually referred to as "small bowel obstruction" and is
abbreviated SBO 
and not SIO) 
then abdominal distention 
can occur please
reword it also 
4. Abstract lines 30 and 31. What is a "cito 
herniotomy procedure"? 
Being a left
sided Amyand's hernia did the patient have situs inversus or 
was there an
extremely mobile cecum?
5. Abstract line 40 I would strongly suggest that this be reworded as " This case
suggests the possibility of early feeding of a banana diet may provoke
incarceration of an inguinal hernia and if the incarcerated hernia contents
contains the appendix, then an Amyand's hernia". 
5 Background page line 8 change in to "into" and reword the next sentence to
"Rarely, the appendix can be found in the inguinal sac of an inguinal hernia
which has been called an Amyand's hernia.
6. Background 
Again line 15. 4 to 6 % is far too high especially with the 1%
described in line 20 this needs to be changed to a more realistic %. 
7. Background line 25 and discussion line 52 change " followed by" to
"associated with" 
and change the word "diver" on line 30 to " differentiate" 
8. Background line 37 change "intestinal obstruction (IBO) " to "small bowel
obstruction( SBI)" this is the more common terminology in the literature
9. Background lines 47 and 50 do you mean "was" or "is"? 
That is, is the banana
diet still common today?
10. Case presentation line 6 and 8 change the word "repositioned" to " reduced"
also line 8 change the word " for" to " from"
11. Case presentation line 33 what do your mean by "excoriated " when
describing the cecum. The figure to me looks like an erythematous cecum not
excoriated; excoriation 
means external trauma with bleeding g from the serosal
surface
12. Case presentation line 38-40. You say " hernia repair" what do you mean by
this. Do you mean just ligation of the sac or did you really try to obliterate the
internal hernia defect ( internal ring- 
that would be quite unusual in a neonate!
13. Discussion line 15 change " could perform 
following" to " can 
develop in an
incarcerated" 
14 discussion lines 23-32. Please define all the types of Amyland's hernia so the
reader understands all the types
15. Discussion lines 35-47 did your patient have situs Inversus ? He obviously
had a mobile cecum you should describe or at the least say it. 
16 discussion is the early banana diet still common in Indonesia today? 
If so this
needs to be stressed and some statement made to highlight the risk of this
especially if the paper by Wiryo and colleagues really did show that the
incidence of SBO was as high as 9%; was this really good epidemiologic study?.
Also more solid foods do not stay as solid food in the small intestine e so the
ability of neonate to "digest" banana implies that there is a large amount of
nondigestable fiber in banana. This needs to be explained.
17. Your title needs to be changed. Your patient did not have appendicitis so
delete the term appendicitis in the title
18 on the conclusions-please make more effort to describe how to educate the
mothers to prevent this complication. Also change the term "morbidity " to "
intestinal obstruction". the term 
morbidity 
is too general.
ADDITIONAL REQUESTS/SUGGESTIONS: 
see above
Publons Reviewer Recognition
. Springer Nature can send verification of this
review directly to Publons (a subsidiary of Clarivate Analytics). If you would
like to take advantage of this service, please click on the “Yes” option below.
Your name, email address, title of the reviewed manuscript, name of the journal,
and date of your review submission (the “Review Data”) will then be transmitted
to Publons after the final decision on the manuscript has been made. If you have
already registered at Publons, they will notify you of the receipt of this review
and update your profile 
as per your settings and their policy. If you are not
registered with Publons, you will receive an email from them asking you to
register in order for them to be able to recognize your review on your new
profile page. Publons may use the Review Data to generate derivative metadata
for the benefit of Publons and you as a reviewer, carefully considering the
sensitivity of such information. For example, Publons may verify your record as
a reviewer by updating your profile published on its webservice if you have
registered for such service or help editors to identify candidate reviewers. Please
find the details of processing in Publons’ privacy policy
https://publons.com/about/terms
No
Declaration of competing interests
Please complete a declaration of competing
interests, considering the following questions:
1
. 
Have you in the past five years received reimbursements, fees, funding, or
salary from an organisation that may in any way gain or lose financially
from the publication of this manuscript, either now or in the future?
2
. 
Do you hold any stocks or shares in an organisation that may in any way
gain or lose financially from the publication of this manuscript, either now
or in the future?
3
. 
Do you hold or are you currently applying for any patents relating to the
content of the manuscript?
4
. 
Have you received reimbursements, fees, funding, or salary from an
organization that holds or has applied for patents relating to the content of
the manuscript?
5
. 
Do you have any other financial competing interests?
6
. 
Do you have any non-financial competing interests in relation to this
paper?
If you can answer no to all of the above, write 'I declare that I have no competing
interests' below. If your reply is yes to any, please give details below.
This reviewer has been recruited by a partner organization, Research Square.
Reviewers with declared or apparent competing interests are not utilized for
these reviews. This reviewer has agreed to publication of their comments online
under a Creative Commons Attribution License attributed to Research Square
and was paid a small honorarium for completing the review within a specified
timeframe. Honoraria for reviews such as this are paid regardless of the reviewer
recommendation.
Reviewer Publication Consent.
 I agree for my report to be made available
under an Open Access Creative Commons CC-BY License (
http://creativecommons.org/licenses/by/4.0
) if this manuscript is accepted for
publication. Any comments that I do not wish to be included in the published
report have been included as confidential comments to the editor, which will not
be published.
If you are not happy for us to publish this report, please contact the
editorial office before completing the review.
If you wish, you can include your
name in your published report. Please note you must decide whether to include
your name at the start of the process and confirm this decision whenever
prompted. If you change your decision later, we will not be able to publish your
name.
I agree to the terms of the CC-BY 4.0 license; please 
do not
 publish my name
with my report. (default)
Is the study design appropriate to answer the research question (including the use
of appropriate controls), and are the conclusions supported by the evidence
presented?
Yes
Are the methods sufficiently described to allow the study to be repeated?
Yes
Is the use of statistics and treatment of uncertainties appropriate?
Yes
Is the presentation of the work clear?
No
Are the images in this manuscript (including electrophoretic gels and blots) free
from apparent manipulation?
Yes
Please review this paper.
user
Reviewer’s report
Title: 
Left-Side Incarcerated Amyand’s Hernia with Appendix and Caecum
provoke by Early Banana Diet: A Case Report
Version: 
1 
Date: 
11 Mar 2021
Reviewer's report:
PEER REVIEWER ASSESSMENTS: 
RELEVANCE - Does this case report make a contribution to medical
knowledge, have educational value, or highlight the need for a change in clinical
practice or diagnostic/prognostic approaches? 
Yes, this report contributes to medical knowledge
CASE DESCRIPTION - Are the details of the case sufficiently well described to
understand the patient's symptoms and course of treatment? 
Yes, the description of the case is sufficient
DIAGNOSIS/INTERPRETATION - Based on the facts presented, are the
diagnosis, interpretation, and course of treatment medically sound?
Yes, the work described is medically sound
DISCUSSION OF THE CASE - Does the discussion appropriately analyse the
importance of the findings and their relevance to future understanding of disease
processes, diagnosis or treatment? Has an adequate literature review pertinent to
the case been included?
No - there are minor issues
OVERALL MANUSCRIPT POTENTIAL - Could an appropriately REVISED
version of this work represent a technically sound contribution?
Probably - with minor revisions
PEER REVIEWER COMMENTS: 
GENERAL COMMENTS: What is your overall impression of the report? 
Note I was not the original reviewer therefore I will review this as a new
submission This submission needs quite a bit of editing for grammar and word
usage BUT it does have a potential tidally important message that an early
banana diet, especially in young children with an inguinal hernia can lead to
bowel incarceration . Also Amyand's hernia usually refers to just the appendix
being trapped in the hernia sac not the cecum as well 
I think this is a minor point
but should be acknowledged
Are you confident in the authors' description and interpretation of the report? 
The description is appropriate but the terminology of a true Amyand's hernia that
differentiates it from a more classic incarcerated inguinal hernia is that it usually
refers to just the appendix being incarcerated in the hernia sac and not the distal
small bowel and cecum. The title though is not accurate the patient did not have
appendicitis at least when I look at the appendix it appears normal
Have the authors clearly outlined the need for publication by novelty of the case
or the specific adverse event? 
Yes I think there is a definite message here for mothers in Indonesia. And for this
reason justifies strong consideration of publication especially is the manuscript
also describes how to educate the population of the importance of not using a
banana diet in neonates !
REQUESTED REVISIONS: 
Suggested changes 
1.please in the future number the pages OR number the lines in a progressive
way-not just a new set of 
numbers for each page ( unless you also number the
pages- this will make it much easier for the reviewer and the editor to address
suggestions to 
individual parts of the manuscript.
2 abstract line 6 
the incidence of 4 to 6% is far too high. Do you mean for
incarcerated hernias! 
Also you can say that this occurs primarily on the right side
in the first sentence and thereby delete the 5th sentence of the abstract. Also
most people think of Amyand's hernia as containing only the appendix
3. 4th sentence of abstract if there is small bowel obstruction( also note small
intestinal obstruction is usually referred to as "small bowel obstruction" and is
abbreviated SBO 
and not SIO) 
then abdominal distention 
can occur please
reword it also 
4. Abstract lines 30 and 31. What is a "cito 
herniotomy procedure"? 
Being a left
sided Amyand's hernia did the patient have situs inversus or 
was there an
extremely mobile cecum?
5. Abstract line 40 I would strongly suggest that this be reworded as " This case
suggests the possibility of early feeding of a banana diet may provoke
incarceration of an inguinal hernia and if the incarcerated hernia contents
contains the appendix, then an Amyand's hernia". 
5 Background page line 8 change in to "into" and reword the next sentence to
"Rarely, the appendix can be found in the inguinal sac of an inguinal hernia
which has been called an Amyand's hernia.
6. Background 
Again line 15. 4 to 6 % is far too high especially with the 1%
described in line 20 this needs to be changed to a more realistic %. 
7. Background line 25 and discussion line 52 change " followed by" to
"associated with" 
and change the word "diver" on line 30 to " differentiate" 
8. Background line 37 change "intestinal obstruction (IBO) " to "small bowel
obstruction( SBI)" this is the more common terminology in the literature
9. Background lines 47 and 50 do you mean "was" or "is"? 
That is, is the banana
diet still common today?
10. Case presentation line 6 and 8 change the word "repositioned" to " reduced"
also line 8 change the word " for" to " from"
11. Case presentation line 33 what do your mean by "excoriated " when
describing the cecum. The figure to me looks like an erythematous cecum not
excoriated; excoriation 
means external trauma with bleeding g from the serosal
surface
12. Case presentation line 38-40. You say " hernia repair" what do you mean by
this. Do you mean just ligation of the sac or did you really try to obliterate the
internal hernia defect ( internal ring- 
that would be quite unusual in a neonate!
13. Discussion line 15 change " could perform 
following" to " can 
develop in an
incarcerated" 
14 discussion lines 23-32. Please define all the types of Amyland's hernia so the
reader understands all the types
15. Discussion lines 35-47 did your patient have situs Inversus ? He obviously
had a mobile cecum you should describe or at the least say it. 
16 discussion is the early banana diet still common in Indonesia today? 
If so this
needs to be stressed and some statement made to highlight the risk of this
especially if the paper by Wiryo and colleagues really did show that the
incidence of SBO was as high as 9%; was this really good epidemiologic study?.
Also more solid foods do not stay as solid food in the small intestine e so the
ability of neonate to "digest" banana implies that there is a large amount of
nondigestable fiber in banana. This needs to be explained.
17. Your title needs to be changed. Your patient did not have appendicitis so
delete the term appendicitis in the title
18 on the conclusions-please make more effort to describe how to educate the
mothers to prevent this complication. Also change the term "morbidity " to "
intestinal obstruction". the term 
morbidity 
is too general.
ADDITIONAL REQUESTS/SUGGESTIONS: 
see above
Publons Reviewer Recognition
. Springer Nature can send verification of this
review directly to Publons (a subsidiary of Clarivate Analytics). If you would
like to take advantage of this service, please click on the “Yes” option below.
Your name, email address, title of the reviewed manuscript, name of the journal,
and date of your review submission (the “Review Data”) will then be transmitted
to Publons after the final decision on the manuscript has been made. If you have
already registered at Publons, they will notify you of the receipt of this review
and update your profile 
as per your settings and their policy. If you are not
registered with Publons, you will receive an email from them asking you to
register in order for them to be able to recognize your review on your new
profile page. Publons may use the Review Data to generate derivative metadata
for the benefit of Publons and you as a reviewer, carefully considering the
sensitivity of such information. For example, Publons may verify your record as
a reviewer by updating your profile published on its webservice if you have
registered for such service or help editors to identify candidate reviewers. Please
find the details of processing in Publons’ privacy policy
https://publons.com/about/terms
No
Declaration of competing interests
Please complete a declaration of competing
interests, considering the following questions:
1
. 
Have you in the past five years received reimbursements, fees, funding, or
salary from an organisation that may in any way gain or lose financially
from the publication of this manuscript, either now or in the future?
2
. 
Do you hold any stocks or shares in an organisation that may in any way
gain or lose financially from the publication of this manuscript, either now
or in the future?
3
. 
Do you hold or are you currently applying for any patents relating to the
content of the manuscript?
4
. 
Have you received reimbursements, fees, funding, or salary from an
organization that holds or has applied for patents relating to the content of
the manuscript?
5
. 
Do you have any other financial competing interests?
6
. 
Do you have any non-financial competing interests in relation to this
paper?
If you can answer no to all of the above, write 'I declare that I have no competing
interests' below. If your reply is yes to any, please give details below.
This reviewer has been recruited by a partner organization, Research Square.
Reviewers with declared or apparent competing interests are not utilized for
these reviews. This reviewer has agreed to publication of their comments online
under a Creative Commons Attribution License attributed to Research Square
and was paid a small honorarium for completing the review within a specified
timeframe. Honoraria for reviews such as this are paid regardless of the reviewer
recommendation.
Reviewer Publication Consent.
 I agree for my report to be made available
under an Open Access Creative Commons CC-BY License (
http://creativecommons.org/licenses/by/4.0
) if this manuscript is accepted for
publication. Any comments that I do not wish to be included in the published
report have been included as confidential comments to the editor, which will not
be published.
If you are not happy for us to publish this report, please contact the
editorial office before completing the review.
If you wish, you can include your
name in your published report. Please note you must decide whether to include
your name at the start of the process and confirm this decision whenever
prompted. If you change your decision later, we will not be able to publish your
name.
I agree to the terms of the CC-BY 4.0 license; please 
do not
 publish my name
with my report. (default)
Is the study design appropriate to answer the research question (including the use
of appropriate controls), and are the conclusions supported by the evidence
presented?
Yes
Are the methods sufficiently described to allow the study to be repeated?
Yes
Is the use of statistics and treatment of uncertainties appropriate?
Yes
Is the presentation of the work clear?
No
Are the images in this manuscript (including electrophoretic gels and blots) free
from apparent manipulation?
Yes
assistant