Reviewer's report
Title:Fucoidan present in brown algae induces apoptosis of HT-29 human colon
cancer cells
Version: 1Date:8 June 2010
Reviewer: Hee-Kyoung Kang
Reviewer's report:
In this manuscript, authors describe the effect of fucoidan on the apoptosis
induction in HT-29 human colon cancer cells. However, following points should
be explained.
Minor Essential Revisions;
1. The present study seems to be similar to the previous report (Hyun et al.,
2009) except cell line. The differences and advanced points of your study
compared to the previous study need to be mentioned.
2. In Hyun’s study, fucoidan at 100 ug/mL induces the apoptosis of HCT-15,
whereas 5-20 ug/mL fucoidan can induce the apoptosis of HT-29 in this study.
HT-29 and HCT-15 are same human colorectalcarcinoma cells. In regard to
apoptosis induction, what can exhibit the concentration difference between the
two cells? Please comment that in the discussion
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Fucoidan present in brown algae induces apoptosis of HT-29 human colon
cancer cells
Version: 1Date:8 June 2010
Reviewer: Hee-Kyoung Kang
Reviewer's report:
In this manuscript, authors describe the effect of fucoidan on the apoptosis
induction in HT-29 human colon cancer cells. However, following points should
be explained.
Minor Essential Revisions;
1. The present study seems to be similar to the previous report (Hyun et al.,
2009) except cell line. The differences and advanced points of your study
compared to the previous study need to be mentioned.
2. In Hyun’s study, fucoidan at 100 ug/mL induces the apoptosis of HCT-15,
whereas 5-20 ug/mL fucoidan can induce the apoptosis of HT-29 in this study.
HT-29 and HCT-15 are same human colorectalcarcinoma cells. In regard to
apoptosis induction, what can exhibit the concentration difference between the
two cells? Please comment that in the discussion
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Fucoidan present in brown algae induces apoptosis of HT-29 human colon
cancer cells
Version: 1Date:8 June 2010
Reviewer: Hee-Kyoung Kang
Reviewer's report:
In this manuscript, authors describe the effect of fucoidan on the apoptosis
induction in HT-29 human colon cancer cells. However, following points should
be explained.
Minor Essential Revisions;
1. The present study seems to be similar to the previous report (Hyun et al.,
2009) except cell line. The differences and advanced points of your study
compared to the previous study need to be mentioned.
2. In Hyun’s study, fucoidan at 100 ug/mL induces the apoptosis of HCT-15,
whereas 5-20 ug/mL fucoidan can induce the apoptosis of HT-29 in this study.
HT-29 and HCT-15 are same human colorectalcarcinoma cells. In regard to
apoptosis induction, what can exhibit the concentration difference between the
two cells? Please comment that in the discussion
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
assistant