Title: PPAR# activation attenuates neonatal CRD-induced visceral pain sensitization and
anxiety in male rats by alleviating oxidative stress
Version: 4 Date: 03 Dec 2024
Reviewer's report:
In Study Design and Methodology Section:
1. The sham group design method for inducing IBS does not appear to be appropriate. It would be
more logical to insert the angioplasty balloon into the colon without applying distention pressure.
2. Reference number 21 describes performing distention on days 8, 10, and 12 with varying
pressures to create the IBS model. Please address the modifications made to the model in your
study and, if possible, explain the rationale behind these changes.
3. To enhance the comparison of results, particularly in the nociceptive test section, it would
have been beneficial to include a naive group in this study.
4. Reconsider the electromyography (EMG) recording method used in the nociceptive test
section. Provide detailed information on the recording frequency, device specifications for
amplifying and filtering signals, high-frequency filter set point, time constant, sampling
frequency, device sensitivity voltage, and scanning speed.
5. Clarify whether spike-to-spike measurements were taken during the evaluation of
electromyography activity.
6. Explain the method used to calculate the percentage of EMG amplitude.
7. Indicate whether the EMG results were statistically analyzed using two-way or one-way
repeated measures ANOVA.
8. Specify the statistical method used for each part of the results in the text and the caption
of the corresponding graph. If two-way repeated measures ANOVA was employed, report the
significance of the interaction between treatment and pressure and the F-value.
9. The internal standard protein and its measurement method were not mentioned in the Western
blot section. Provide a more detailed description of the protein quantification method used.
In Discussion Section:
1. Article No. 51 is a comprehensive review on the role of PPAR-gamma in irritable
bowel syndrome. Numerous experimental studies have induced IBS using various methods
and demonstrated the role of PPAR-gamma and its agonists in controlling allodynia and
inflammation. These findings were not reviewed or compared in the discussion of the current
article. It would be beneficial to highlight the possible advantages or disadvantages of your study
by referring to these other studies.
2. One of the findings of the present study is anxiety-like symptoms in mice. The discussion of
this result is inadequately written, and the cited reference (No. 55) is not strongly related. This
section should be rewritten to better discuss the observed anxiety-like symptoms.
Title: PPAR# activation attenuates neonatal CRD-induced visceral pain sensitization and
anxiety in male rats by alleviating oxidative stress
Version: 4 Date: 03 Dec 2024
Reviewer's report:
In Study Design and Methodology Section:
1. The sham group design method for inducing IBS does not appear to be appropriate. It would be
more logical to insert the angioplasty balloon into the colon without applying distention pressure.
2. Reference number 21 describes performing distention on days 8, 10, and 12 with varying
pressures to create the IBS model. Please address the modifications made to the model in your
study and, if possible, explain the rationale behind these changes.
3. To enhance the comparison of results, particularly in the nociceptive test section, it would
have been beneficial to include a naive group in this study.
4. Reconsider the electromyography (EMG) recording method used in the nociceptive test
section. Provide detailed information on the recording frequency, device specifications for
amplifying and filtering signals, high-frequency filter set point, time constant, sampling
frequency, device sensitivity voltage, and scanning speed.
5. Clarify whether spike-to-spike measurements were taken during the evaluation of
electromyography activity.
6. Explain the method used to calculate the percentage of EMG amplitude.
7. Indicate whether the EMG results were statistically analyzed using two-way or one-way
repeated measures ANOVA.
8. Specify the statistical method used for each part of the results in the text and the caption
of the corresponding graph. If two-way repeated measures ANOVA was employed, report the
significance of the interaction between treatment and pressure and the F-value.
9. The internal standard protein and its measurement method were not mentioned in the Western
blot section. Provide a more detailed description of the protein quantification method used.
In Discussion Section:
1. Article No. 51 is a comprehensive review on the role of PPAR-gamma in irritable
bowel syndrome. Numerous experimental studies have induced IBS using various methods
and demonstrated the role of PPAR-gamma and its agonists in controlling allodynia and
inflammation. These findings were not reviewed or compared in the discussion of the current
article. It would be beneficial to highlight the possible advantages or disadvantages of your study
by referring to these other studies.
2. One of the findings of the present study is anxiety-like symptoms in mice. The discussion of
this result is inadequately written, and the cited reference (No. 55) is not strongly related. This
section should be rewritten to better discuss the observed anxiety-like symptoms.
Please review this paper.
user
Title: PPAR# activation attenuates neonatal CRD-induced visceral pain sensitization and
anxiety in male rats by alleviating oxidative stress
Version: 4 Date: 03 Dec 2024
Reviewer's report:
In Study Design and Methodology Section:
1. The sham group design method for inducing IBS does not appear to be appropriate. It would be
more logical to insert the angioplasty balloon into the colon without applying distention pressure.
2. Reference number 21 describes performing distention on days 8, 10, and 12 with varying
pressures to create the IBS model. Please address the modifications made to the model in your
study and, if possible, explain the rationale behind these changes.
3. To enhance the comparison of results, particularly in the nociceptive test section, it would
have been beneficial to include a naive group in this study.
4. Reconsider the electromyography (EMG) recording method used in the nociceptive test
section. Provide detailed information on the recording frequency, device specifications for
amplifying and filtering signals, high-frequency filter set point, time constant, sampling
frequency, device sensitivity voltage, and scanning speed.
5. Clarify whether spike-to-spike measurements were taken during the evaluation of
electromyography activity.
6. Explain the method used to calculate the percentage of EMG amplitude.
7. Indicate whether the EMG results were statistically analyzed using two-way or one-way
repeated measures ANOVA.
8. Specify the statistical method used for each part of the results in the text and the caption
of the corresponding graph. If two-way repeated measures ANOVA was employed, report the
significance of the interaction between treatment and pressure and the F-value.
9. The internal standard protein and its measurement method were not mentioned in the Western
blot section. Provide a more detailed description of the protein quantification method used.
In Discussion Section:
1. Article No. 51 is a comprehensive review on the role of PPAR-gamma in irritable
bowel syndrome. Numerous experimental studies have induced IBS using various methods
and demonstrated the role of PPAR-gamma and its agonists in controlling allodynia and
inflammation. These findings were not reviewed or compared in the discussion of the current
article. It would be beneficial to highlight the possible advantages or disadvantages of your study
by referring to these other studies.
2. One of the findings of the present study is anxiety-like symptoms in mice. The discussion of
this result is inadequately written, and the cited reference (No. 55) is not strongly related. This
section should be rewritten to better discuss the observed anxiety-like symptoms.
assistant