Reviewer's report
Title:Laxative effects of Salecan on loperamide-induced constipation in mice
Version: 2Date:27 December 2012
Reviewer: fenghong huang
Reviewer's report:
This piece of work was previously submitted to the journal for consideration. I
was one of the original reviewers.
Major Compulsory Revisions
The authors have failed to address many of the major issues with the first
submission highlighted by both reviewers in their second submission. In their
response to these comments, the authors have given appropriate rationale, yet
failed to highlight these in the second draft of the manuscript.
1. The authors can not explain clearly the rationale for measuring gastric
emptying in this study. If there is no direct relationship between gastric emptying
and constipation, the test of gastric emptying is redundant, as the purpose of the
study is to assess constipation and not intestinal transit.
2. This study tries to assess the effect of salecan on constipation, why did the
authors test some parameters in normal mice.
3. There are lots of statements about short chain fatty acids in section of
discussion. Soluble fiber can produce short chain fatty acids in colon. The
authors should provide evident about salecan and the producing of short chain
fatty acids.
4. In previous review, I have asked the authors to state whether the mice were
treated before the experiment. Feeding interfere all test in this study, so fast is
necessary for this study. But the authors’ answer is disappointing.
Level of interest: An article of limited interest
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Laxative effects of Salecan on loperamide-induced constipation in mice
Version: 2Date:27 December 2012
Reviewer: fenghong huang
Reviewer's report:
This piece of work was previously submitted to the journal for consideration. I
was one of the original reviewers.
Major Compulsory Revisions
The authors have failed to address many of the major issues with the first
submission highlighted by both reviewers in their second submission. In their
response to these comments, the authors have given appropriate rationale, yet
failed to highlight these in the second draft of the manuscript.
1. The authors can not explain clearly the rationale for measuring gastric
emptying in this study. If there is no direct relationship between gastric emptying
and constipation, the test of gastric emptying is redundant, as the purpose of the
study is to assess constipation and not intestinal transit.
2. This study tries to assess the effect of salecan on constipation, why did the
authors test some parameters in normal mice.
3. There are lots of statements about short chain fatty acids in section of
discussion. Soluble fiber can produce short chain fatty acids in colon. The
authors should provide evident about salecan and the producing of short chain
fatty acids.
4. In previous review, I have asked the authors to state whether the mice were
treated before the experiment. Feeding interfere all test in this study, so fast is
necessary for this study. But the authors’ answer is disappointing.
Level of interest: An article of limited interest
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Laxative effects of Salecan on loperamide-induced constipation in mice
Version: 2Date:27 December 2012
Reviewer: fenghong huang
Reviewer's report:
This piece of work was previously submitted to the journal for consideration. I
was one of the original reviewers.
Major Compulsory Revisions
The authors have failed to address many of the major issues with the first
submission highlighted by both reviewers in their second submission. In their
response to these comments, the authors have given appropriate rationale, yet
failed to highlight these in the second draft of the manuscript.
1. The authors can not explain clearly the rationale for measuring gastric
emptying in this study. If there is no direct relationship between gastric emptying
and constipation, the test of gastric emptying is redundant, as the purpose of the
study is to assess constipation and not intestinal transit.
2. This study tries to assess the effect of salecan on constipation, why did the
authors test some parameters in normal mice.
3. There are lots of statements about short chain fatty acids in section of
discussion. Soluble fiber can produce short chain fatty acids in colon. The
authors should provide evident about salecan and the producing of short chain
fatty acids.
4. In previous review, I have asked the authors to state whether the mice were
treated before the experiment. Feeding interfere all test in this study, so fast is
necessary for this study. But the authors’ answer is disappointing.
Level of interest: An article of limited interest
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
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