Reviewer's report 
 Decreased Expression of Cytochrome P450 Protein in Non-Malignant Colonic Tissue of Title: 
Patients with Colonic Adenoma 
 1  28 July 2005 Version: Date: 
 james Hardwick Reviewer: 
Reviewer's report: 
General 
--------------------------------------------------------------------------------------------------------------------------------------------------------- 
Minor Essential Revisions (such as missing labels on figures, or the wrong use of a term, which the 
author can be trusted to correct) 
------------------------------------------------------------------------------- 
Background is limited and should be expanded to include the reason for this study and the 
importance of P450 metabolism in chemotherpeutic treatment of colorectal cancer. 
Both the text and figure should be more clearly defined in regards to normal tissue of adenoma 
patients and normal colon tissue of disease free individuals. Figure 3 patient and control should be 
clarified as adenoma patient normal colon tissue vs normal colon tissue from disease free individual. 
Figure should include the level of actin or control protein for each western immunoblot. 
Should include a figure of RT-PCR analysis of P450 mRNA in adenoma, normal colon tissue from 
patient and disease free individual. Expand Figure 1 B or include a table with relative changes in mRNA in individual patients. 
Table 4 should include the relative level of mRNA to the different P450 not whether a amplified band 
is present or not. 
The discussion should also include information on the polymorphic expression of CYP gene in colon 
tissue and importance in drug metabolism. 
 Accept after minor essential revisions What next?: 
 An article whose findings are important to those with closely related research Level of interest: 
interests 
 Acceptable Quality of written English: 
 No Statistical review: 
Declaration of competing interests: 
I declare that I have no competing interests 
Reviewer's report 
 Decreased Expression of Cytochrome P450 Protein in Non-Malignant Colonic Tissue of Title: 
Patients with Colonic Adenoma 
 1  28 July 2005 Version: Date: 
 james Hardwick Reviewer: 
Reviewer's report: 
General 
--------------------------------------------------------------------------------------------------------------------------------------------------------- 
Minor Essential Revisions (such as missing labels on figures, or the wrong use of a term, which the 
author can be trusted to correct) 
------------------------------------------------------------------------------- 
Background is limited and should be expanded to include the reason for this study and the 
importance of P450 metabolism in chemotherpeutic treatment of colorectal cancer. 
Both the text and figure should be more clearly defined in regards to normal tissue of adenoma 
patients and normal colon tissue of disease free individuals. Figure 3 patient and control should be 
clarified as adenoma patient normal colon tissue vs normal colon tissue from disease free individual. 
Figure should include the level of actin or control protein for each western immunoblot. 
Should include a figure of RT-PCR analysis of P450 mRNA in adenoma, normal colon tissue from 
patient and disease free individual. Expand Figure 1 B or include a table with relative changes in mRNA in individual patients. 
Table 4 should include the relative level of mRNA to the different P450 not whether a amplified band 
is present or not. 
The discussion should also include information on the polymorphic expression of CYP gene in colon 
tissue and importance in drug metabolism. 
 Accept after minor essential revisions What next?: 
 An article whose findings are important to those with closely related research Level of interest: 
interests 
 Acceptable Quality of written English: 
 No Statistical review: 
Declaration of competing interests: 
I declare that I have no competing interests 
Please review this paper.
user
Reviewer's report 
 Decreased Expression of Cytochrome P450 Protein in Non-Malignant Colonic Tissue of Title: 
Patients with Colonic Adenoma 
 1  28 July 2005 Version: Date: 
 james Hardwick Reviewer: 
Reviewer's report: 
General 
--------------------------------------------------------------------------------------------------------------------------------------------------------- 
Minor Essential Revisions (such as missing labels on figures, or the wrong use of a term, which the 
author can be trusted to correct) 
------------------------------------------------------------------------------- 
Background is limited and should be expanded to include the reason for this study and the 
importance of P450 metabolism in chemotherpeutic treatment of colorectal cancer. 
Both the text and figure should be more clearly defined in regards to normal tissue of adenoma 
patients and normal colon tissue of disease free individuals. Figure 3 patient and control should be 
clarified as adenoma patient normal colon tissue vs normal colon tissue from disease free individual. 
Figure should include the level of actin or control protein for each western immunoblot. 
Should include a figure of RT-PCR analysis of P450 mRNA in adenoma, normal colon tissue from 
patient and disease free individual. Expand Figure 1 B or include a table with relative changes in mRNA in individual patients. 
Table 4 should include the relative level of mRNA to the different P450 not whether a amplified band 
is present or not. 
The discussion should also include information on the polymorphic expression of CYP gene in colon 
tissue and importance in drug metabolism. 
 Accept after minor essential revisions What next?: 
 An article whose findings are important to those with closely related research Level of interest: 
interests 
 Acceptable Quality of written English: 
 No Statistical review: 
Declaration of competing interests: 
I declare that I have no competing interests 
assistant