Reviewer's report
Title:Effects of Interleukin-4 or Interleukin-10 Gene Therapy on
Trinitrobenzenesulfonic Acid-Induced Mouse Colitis
Version: 1Date:18 July 2013
Reviewer: Chien-Yun Hsiang
Reviewer's report:
This manuscript reports the effects of IL-4 or IL-10 gene therapy on
TNBS-induced mouse colitis. Authors find that intraperitoneal injection of plasmid
DNA carrying IL-4 or IL-10 gene is useful in the control of TNBS-induced murine
colitis. However, their combination has less effect on the TNBS-induced colitis.
Finding a way to control IBD is an important issue. This work suggests the
potential usage of IL-4 or IL-10 on the improvement of TNBS-induced colitis.
However, there are some questions needed to be addressed.
1. There are many studies reporting the usage of IL-4 or IL-10 protein on the
treatment of TNBS-induced colitis. Therefore, authors should emphasize the
novelity of this manuscript.
2. The effects of IL-4 or IL-10 on the improvement of TNBS-induced colitis are
controversial. Some reports show that administration of these cytokines is
effective but others don't. The detail mechanisms of IL-4 or IL-10 on the colitis
should be further analyzed.
3. Authors find that combination of IL-4 and IL-10 has less effect on colitis.
Recent study reports that IL-4 induces a suppressive IL-10-producing CD8+ T
cell population. Authors should further elucidate why combinatory therapy shows
less effect.
4. In this manuscript, plasmid DNA carrying IL-4 or IL-10 gene is injected
intraperitoneally and RT-PCR data show that IL-4 and IL-10 RNAs are detectable
in the colon. How do these plasmid DNAs be delivered to colon, uptaken by
colon cells, and expressed in colon? What kinds of cells in the colon are
responsible for the expression of exogenous IL-4 or IL-10 gene? Authors should
elucidate the kinds of cells expressing IL-4 or IL-10 by IHC or other assays.
Additionally, the delivery of plasmid DNA to colon is another question needed to
be analyzed.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
'I declare that I have no competing interests'
Reviewer's report
Title:Effects of Interleukin-4 or Interleukin-10 Gene Therapy on
Trinitrobenzenesulfonic Acid-Induced Mouse Colitis
Version: 1Date:18 July 2013
Reviewer: Chien-Yun Hsiang
Reviewer's report:
This manuscript reports the effects of IL-4 or IL-10 gene therapy on
TNBS-induced mouse colitis. Authors find that intraperitoneal injection of plasmid
DNA carrying IL-4 or IL-10 gene is useful in the control of TNBS-induced murine
colitis. However, their combination has less effect on the TNBS-induced colitis.
Finding a way to control IBD is an important issue. This work suggests the
potential usage of IL-4 or IL-10 on the improvement of TNBS-induced colitis.
However, there are some questions needed to be addressed.
1. There are many studies reporting the usage of IL-4 or IL-10 protein on the
treatment of TNBS-induced colitis. Therefore, authors should emphasize the
novelity of this manuscript.
2. The effects of IL-4 or IL-10 on the improvement of TNBS-induced colitis are
controversial. Some reports show that administration of these cytokines is
effective but others don't. The detail mechanisms of IL-4 or IL-10 on the colitis
should be further analyzed.
3. Authors find that combination of IL-4 and IL-10 has less effect on colitis.
Recent study reports that IL-4 induces a suppressive IL-10-producing CD8+ T
cell population. Authors should further elucidate why combinatory therapy shows
less effect.
4. In this manuscript, plasmid DNA carrying IL-4 or IL-10 gene is injected
intraperitoneally and RT-PCR data show that IL-4 and IL-10 RNAs are detectable
in the colon. How do these plasmid DNAs be delivered to colon, uptaken by
colon cells, and expressed in colon? What kinds of cells in the colon are
responsible for the expression of exogenous IL-4 or IL-10 gene? Authors should
elucidate the kinds of cells expressing IL-4 or IL-10 by IHC or other assays.
Additionally, the delivery of plasmid DNA to colon is another question needed to
be analyzed.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
'I declare that I have no competing interests'
Please review this paper.
user
Reviewer's report
Title:Effects of Interleukin-4 or Interleukin-10 Gene Therapy on
Trinitrobenzenesulfonic Acid-Induced Mouse Colitis
Version: 1Date:18 July 2013
Reviewer: Chien-Yun Hsiang
Reviewer's report:
This manuscript reports the effects of IL-4 or IL-10 gene therapy on
TNBS-induced mouse colitis. Authors find that intraperitoneal injection of plasmid
DNA carrying IL-4 or IL-10 gene is useful in the control of TNBS-induced murine
colitis. However, their combination has less effect on the TNBS-induced colitis.
Finding a way to control IBD is an important issue. This work suggests the
potential usage of IL-4 or IL-10 on the improvement of TNBS-induced colitis.
However, there are some questions needed to be addressed.
1. There are many studies reporting the usage of IL-4 or IL-10 protein on the
treatment of TNBS-induced colitis. Therefore, authors should emphasize the
novelity of this manuscript.
2. The effects of IL-4 or IL-10 on the improvement of TNBS-induced colitis are
controversial. Some reports show that administration of these cytokines is
effective but others don't. The detail mechanisms of IL-4 or IL-10 on the colitis
should be further analyzed.
3. Authors find that combination of IL-4 and IL-10 has less effect on colitis.
Recent study reports that IL-4 induces a suppressive IL-10-producing CD8+ T
cell population. Authors should further elucidate why combinatory therapy shows
less effect.
4. In this manuscript, plasmid DNA carrying IL-4 or IL-10 gene is injected
intraperitoneally and RT-PCR data show that IL-4 and IL-10 RNAs are detectable
in the colon. How do these plasmid DNAs be delivered to colon, uptaken by
colon cells, and expressed in colon? What kinds of cells in the colon are
responsible for the expression of exogenous IL-4 or IL-10 gene? Authors should
elucidate the kinds of cells expressing IL-4 or IL-10 by IHC or other assays.
Additionally, the delivery of plasmid DNA to colon is another question needed to
be analyzed.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
'I declare that I have no competing interests'
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