Reviewer's report
Title:The role of prostaglandin E2 (PGE2) in toll-like receptor 4 (TLR4)-mediated
colitis-associated neoplasia
Version: 3Date:10 May 2010
Reviewer: Naofumi Mukaida
Reviewer's report:
It is widely known that COX-2 is downstream of TLR-4 and that COX-2-derived
prostaglandin (PG) E2 plays a central role in colon carcinogenesis. The authors
speculated that exogenous PGE2 disturb the balance between prostaglandin E2
and 15d-PGJ2, thereby accelerating colon carcinogenesis, but they failed to
provide any experimental evidence. Moreover, the authors demonstrated that
PGE2 could increase the amount of AR and phosphorylation of EGF receptor
without any effects on proliferation and apoptosis of colon epithelial cells. In
conclusion, the authors failed to provide any definitive mechanistic insights on
how PGE2 can promote colon carcinogenesis, even in this revised text.
Level of interest: An article of limited interest
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Reviewer's report
Title:The role of prostaglandin E2 (PGE2) in toll-like receptor 4 (TLR4)-mediated
colitis-associated neoplasia
Version: 3Date:10 May 2010
Reviewer: Naofumi Mukaida
Reviewer's report:
It is widely known that COX-2 is downstream of TLR-4 and that COX-2-derived
prostaglandin (PG) E2 plays a central role in colon carcinogenesis. The authors
speculated that exogenous PGE2 disturb the balance between prostaglandin E2
and 15d-PGJ2, thereby accelerating colon carcinogenesis, but they failed to
provide any experimental evidence. Moreover, the authors demonstrated that
PGE2 could increase the amount of AR and phosphorylation of EGF receptor
without any effects on proliferation and apoptosis of colon epithelial cells. In
conclusion, the authors failed to provide any definitive mechanistic insights on
how PGE2 can promote colon carcinogenesis, even in this revised text.
Level of interest: An article of limited interest
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Please review this paper.
user
Reviewer's report
Title:The role of prostaglandin E2 (PGE2) in toll-like receptor 4 (TLR4)-mediated
colitis-associated neoplasia
Version: 3Date:10 May 2010
Reviewer: Naofumi Mukaida
Reviewer's report:
It is widely known that COX-2 is downstream of TLR-4 and that COX-2-derived
prostaglandin (PG) E2 plays a central role in colon carcinogenesis. The authors
speculated that exogenous PGE2 disturb the balance between prostaglandin E2
and 15d-PGJ2, thereby accelerating colon carcinogenesis, but they failed to
provide any experimental evidence. Moreover, the authors demonstrated that
PGE2 could increase the amount of AR and phosphorylation of EGF receptor
without any effects on proliferation and apoptosis of colon epithelial cells. In
conclusion, the authors failed to provide any definitive mechanistic insights on
how PGE2 can promote colon carcinogenesis, even in this revised text.
Level of interest: An article of limited interest
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
assistant