Reviewer's report 
 Epidermal growth factor mediates detachment from and invasion through collagen I and Title: 
Matrigel in Capan-1 pancreatic cancer cells 
 2  17 January 2005 Version: Date: 
 Surinder Batra Reviewer: 
Reviewer's report: 
The revised manuscript has clarified several points raised by the reviewers in the first submission. 
As pointed by other reviewer and the authors, there are differences between various reports on the 
expression of EGFR1 and EGFR2 in Capan 1 cells; therefore, it is important to show their 
expression, including EGFR3 and EGFR4 in these cells (including MiaPaca) in this manuscript. 
Additionally, a large number of Figures are included in this manuscript and can be reduced. Figure 
10 and 11 can become part of Figures with Capan 1. There are different nomenclature, for example, 
vehicle, no treatment or control. It should be same for clarity. Results on MiaPaca should be 
integrated with Capan 1 and explanations should be provided for differences on the percentage of 
adhesion values. In summary, the manuscript is recommended for publication contingent upon completion of the changes requested above. 
Reviewer's report 
 Epidermal growth factor mediates detachment from and invasion through collagen I and Title: 
Matrigel in Capan-1 pancreatic cancer cells 
 2  17 January 2005 Version: Date: 
 Surinder Batra Reviewer: 
Reviewer's report: 
The revised manuscript has clarified several points raised by the reviewers in the first submission. 
As pointed by other reviewer and the authors, there are differences between various reports on the 
expression of EGFR1 and EGFR2 in Capan 1 cells; therefore, it is important to show their 
expression, including EGFR3 and EGFR4 in these cells (including MiaPaca) in this manuscript. 
Additionally, a large number of Figures are included in this manuscript and can be reduced. Figure 
10 and 11 can become part of Figures with Capan 1. There are different nomenclature, for example, 
vehicle, no treatment or control. It should be same for clarity. Results on MiaPaca should be 
integrated with Capan 1 and explanations should be provided for differences on the percentage of 
adhesion values. In summary, the manuscript is recommended for publication contingent upon completion of the changes requested above. 
Please review this paper.
user
Reviewer's report 
 Epidermal growth factor mediates detachment from and invasion through collagen I and Title: 
Matrigel in Capan-1 pancreatic cancer cells 
 2  17 January 2005 Version: Date: 
 Surinder Batra Reviewer: 
Reviewer's report: 
The revised manuscript has clarified several points raised by the reviewers in the first submission. 
As pointed by other reviewer and the authors, there are differences between various reports on the 
expression of EGFR1 and EGFR2 in Capan 1 cells; therefore, it is important to show their 
expression, including EGFR3 and EGFR4 in these cells (including MiaPaca) in this manuscript. 
Additionally, a large number of Figures are included in this manuscript and can be reduced. Figure 
10 and 11 can become part of Figures with Capan 1. There are different nomenclature, for example, 
vehicle, no treatment or control. It should be same for clarity. Results on MiaPaca should be 
integrated with Capan 1 and explanations should be provided for differences on the percentage of 
adhesion values. In summary, the manuscript is recommended for publication contingent upon completion of the changes requested above. 
assistant