Reviewer's report
Title:Over-expression of COX-2 mRNA in colorectal cancer
Version: 1Date:26 August 2013
Reviewer: li liang
Reviewer's report:
Major compulsory revisions:
1. The authors performed qPCR to detect the mRNA level of COX-2 relative to
tissue weight or the mRNA levels of two housekeeping genes in 60 paired CRC
tissues. They did not mention the quality of mRNAs extracted from tissues.
Moreover, it is too simple for experimental design. The changes of COX-2 protein
levels should be shown by Western blotting in the same CRC tissues.
2. The clinicopathological analyses of COX-2 in 60 paired CRC tissues should be
shown in a table.
3. The figure of COX-2 expression in CRC tissues is not intuitive. Figures with
bar may be better.
Minor essential revisions
1. There are many grammar and careless mistakes. Some sentences are hard to
read. Authors should have the manuscript edited by a native English speaker
with scientific experience.
Level of interest: An article of limited interest
Quality of written English: Not suitable for publication unless extensively edited
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests' below.
Reviewer's report
Title:Over-expression of COX-2 mRNA in colorectal cancer
Version: 1Date:26 August 2013
Reviewer: li liang
Reviewer's report:
Major compulsory revisions:
1. The authors performed qPCR to detect the mRNA level of COX-2 relative to
tissue weight or the mRNA levels of two housekeeping genes in 60 paired CRC
tissues. They did not mention the quality of mRNAs extracted from tissues.
Moreover, it is too simple for experimental design. The changes of COX-2 protein
levels should be shown by Western blotting in the same CRC tissues.
2. The clinicopathological analyses of COX-2 in 60 paired CRC tissues should be
shown in a table.
3. The figure of COX-2 expression in CRC tissues is not intuitive. Figures with
bar may be better.
Minor essential revisions
1. There are many grammar and careless mistakes. Some sentences are hard to
read. Authors should have the manuscript edited by a native English speaker
with scientific experience.
Level of interest: An article of limited interest
Quality of written English: Not suitable for publication unless extensively edited
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests' below.
Please review this paper.
user
Reviewer's report
Title:Over-expression of COX-2 mRNA in colorectal cancer
Version: 1Date:26 August 2013
Reviewer: li liang
Reviewer's report:
Major compulsory revisions:
1. The authors performed qPCR to detect the mRNA level of COX-2 relative to
tissue weight or the mRNA levels of two housekeeping genes in 60 paired CRC
tissues. They did not mention the quality of mRNAs extracted from tissues.
Moreover, it is too simple for experimental design. The changes of COX-2 protein
levels should be shown by Western blotting in the same CRC tissues.
2. The clinicopathological analyses of COX-2 in 60 paired CRC tissues should be
shown in a table.
3. The figure of COX-2 expression in CRC tissues is not intuitive. Figures with
bar may be better.
Minor essential revisions
1. There are many grammar and careless mistakes. Some sentences are hard to
read. Authors should have the manuscript edited by a native English speaker
with scientific experience.
Level of interest: An article of limited interest
Quality of written English: Not suitable for publication unless extensively edited
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests' below.
assistant