Reviewer's report
Title:MDR1 polymorphisms are associated with inflammatory bowel disease in a
cohort of Croatian IBD patients
Version: 1Date:21 October 2012
Reviewer: Alfreda Krupoves
Reviewer's report:
The authors carried out a case-control genetic association study in adult IBD
patient’s population. This paper is an interesting addition to the body of research
on this topic. Please find below the aspect to be improved.
Major Compulsory Revisions:
1.The process of controls selection and sample collection should be described,
as well as the possibility of genotyping errors and what attempts were made
aiming to prevent it. The period of time during which patients were followed or
recruited should be provided.
2.This paper would benefit from discussion of study internal validity (to add in the
“discussion” section). The possibility of bias should be discussed. Given small
sample size and generally low magnitude (OR < 1,5) of associations in
gene-disease studies, a power analysis should be made and presented in order
to give an idea about the magnitude of association study was adequately
powered to detect.
3.The detected association with C3435T is a silent substitution, so its role in
pathogenesis should be discussed, what is the biological plausibility supporting
this association.
4.Authors state that some (not anticipated) associations were found between
phenotype of extra intestinal manifestations, but these EM are not described in
details. What was the definition of EM should be clarified.
Minor Essential Revisions:
1.A table for the presentation of results of associations between the disease
characteristics and studied snps would be useful as well.
2.In order to render the table self-explanatory, all abbreviations have to be
specified in table foot note.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests' below
Reviewer's report
Title:MDR1 polymorphisms are associated with inflammatory bowel disease in a
cohort of Croatian IBD patients
Version: 1Date:21 October 2012
Reviewer: Alfreda Krupoves
Reviewer's report:
The authors carried out a case-control genetic association study in adult IBD
patient’s population. This paper is an interesting addition to the body of research
on this topic. Please find below the aspect to be improved.
Major Compulsory Revisions:
1.The process of controls selection and sample collection should be described,
as well as the possibility of genotyping errors and what attempts were made
aiming to prevent it. The period of time during which patients were followed or
recruited should be provided.
2.This paper would benefit from discussion of study internal validity (to add in the
“discussion” section). The possibility of bias should be discussed. Given small
sample size and generally low magnitude (OR < 1,5) of associations in
gene-disease studies, a power analysis should be made and presented in order
to give an idea about the magnitude of association study was adequately
powered to detect.
3.The detected association with C3435T is a silent substitution, so its role in
pathogenesis should be discussed, what is the biological plausibility supporting
this association.
4.Authors state that some (not anticipated) associations were found between
phenotype of extra intestinal manifestations, but these EM are not described in
details. What was the definition of EM should be clarified.
Minor Essential Revisions:
1.A table for the presentation of results of associations between the disease
characteristics and studied snps would be useful as well.
2.In order to render the table self-explanatory, all abbreviations have to be
specified in table foot note.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests' below
Please review this paper.
user
Reviewer's report
Title:MDR1 polymorphisms are associated with inflammatory bowel disease in a
cohort of Croatian IBD patients
Version: 1Date:21 October 2012
Reviewer: Alfreda Krupoves
Reviewer's report:
The authors carried out a case-control genetic association study in adult IBD
patient’s population. This paper is an interesting addition to the body of research
on this topic. Please find below the aspect to be improved.
Major Compulsory Revisions:
1.The process of controls selection and sample collection should be described,
as well as the possibility of genotyping errors and what attempts were made
aiming to prevent it. The period of time during which patients were followed or
recruited should be provided.
2.This paper would benefit from discussion of study internal validity (to add in the
“discussion” section). The possibility of bias should be discussed. Given small
sample size and generally low magnitude (OR < 1,5) of associations in
gene-disease studies, a power analysis should be made and presented in order
to give an idea about the magnitude of association study was adequately
powered to detect.
3.The detected association with C3435T is a silent substitution, so its role in
pathogenesis should be discussed, what is the biological plausibility supporting
this association.
4.Authors state that some (not anticipated) associations were found between
phenotype of extra intestinal manifestations, but these EM are not described in
details. What was the definition of EM should be clarified.
Minor Essential Revisions:
1.A table for the presentation of results of associations between the disease
characteristics and studied snps would be useful as well.
2.In order to render the table self-explanatory, all abbreviations have to be
specified in table foot note.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests' below
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