Title: Emodin Protects Against Severe Acute Pancreatitis-associated Acute Lung Injury by
Activating Nrf2/HO-1/GPX4 Signal and Inhibiting Ferroptosis in Vivo and in Vitro
Version: 2 Date: 04 Jan 2025
Reviewer's report:
This is a revised version of a manuscript by Shen et al. in which a research article to explore how
EMO protects against lung injury in SAP rats and alveolar epithelial cells, concentrating on the
classical ferroptosis pathway. The authors show relevant data in vitro and in vivo. The study is
sound, and the protocol detailed enough for others to replicate. In my opinion, the authors have
addressed the concerns raised by my colleague reviewers, but I do have some minor concerns/
comments for the authors:
I found a couple of petty typos and minor comments for the authors to fix:
Minor Comments:
1. Line 107-line 109: “The SO group, SAP group, SAP+EMO group, SAP+Nrf2 inhibitor
group (SAP+ML385 group), and SAP+ML385+EMO group were randomly assigned to forty
SD rats” should be written as “Forty SD rats were randomly assigned into five groups: SO
group, SAP group, SAP+EMO group, SAP+Nrf2 inhibitor group (SAP+ML385 group), and
SAP+ML385+EMO group”.
2. In future study, it would be better to include both male and female subjects.
3. Line 131: the title of Figure 1 should be changed to “Experimental design schematic diagram.
(A) In vivo study design. (B) In vitro study design.”
4. Line 133: it should be changed to “Chemicals and Reagents.”
5. Line 193: “PyMol” instead of “Pymol”
6. Line 256: the title of “3.1” is a little bit confused, and please rewrite it.
Title: Emodin Protects Against Severe Acute Pancreatitis-associated Acute Lung Injury by
Activating Nrf2/HO-1/GPX4 Signal and Inhibiting Ferroptosis in Vivo and in Vitro
Version: 2 Date: 04 Jan 2025
Reviewer's report:
This is a revised version of a manuscript by Shen et al. in which a research article to explore how
EMO protects against lung injury in SAP rats and alveolar epithelial cells, concentrating on the
classical ferroptosis pathway. The authors show relevant data in vitro and in vivo. The study is
sound, and the protocol detailed enough for others to replicate. In my opinion, the authors have
addressed the concerns raised by my colleague reviewers, but I do have some minor concerns/
comments for the authors:
I found a couple of petty typos and minor comments for the authors to fix:
Minor Comments:
1. Line 107-line 109: “The SO group, SAP group, SAP+EMO group, SAP+Nrf2 inhibitor
group (SAP+ML385 group), and SAP+ML385+EMO group were randomly assigned to forty
SD rats” should be written as “Forty SD rats were randomly assigned into five groups: SO
group, SAP group, SAP+EMO group, SAP+Nrf2 inhibitor group (SAP+ML385 group), and
SAP+ML385+EMO group”.
2. In future study, it would be better to include both male and female subjects.
3. Line 131: the title of Figure 1 should be changed to “Experimental design schematic diagram.
(A) In vivo study design. (B) In vitro study design.”
4. Line 133: it should be changed to “Chemicals and Reagents.”
5. Line 193: “PyMol” instead of “Pymol”
6. Line 256: the title of “3.1” is a little bit confused, and please rewrite it.
Please review this paper.
user
Title: Emodin Protects Against Severe Acute Pancreatitis-associated Acute Lung Injury by
Activating Nrf2/HO-1/GPX4 Signal and Inhibiting Ferroptosis in Vivo and in Vitro
Version: 2 Date: 04 Jan 2025
Reviewer's report:
This is a revised version of a manuscript by Shen et al. in which a research article to explore how
EMO protects against lung injury in SAP rats and alveolar epithelial cells, concentrating on the
classical ferroptosis pathway. The authors show relevant data in vitro and in vivo. The study is
sound, and the protocol detailed enough for others to replicate. In my opinion, the authors have
addressed the concerns raised by my colleague reviewers, but I do have some minor concerns/
comments for the authors:
I found a couple of petty typos and minor comments for the authors to fix:
Minor Comments:
1. Line 107-line 109: “The SO group, SAP group, SAP+EMO group, SAP+Nrf2 inhibitor
group (SAP+ML385 group), and SAP+ML385+EMO group were randomly assigned to forty
SD rats” should be written as “Forty SD rats were randomly assigned into five groups: SO
group, SAP group, SAP+EMO group, SAP+Nrf2 inhibitor group (SAP+ML385 group), and
SAP+ML385+EMO group”.
2. In future study, it would be better to include both male and female subjects.
3. Line 131: the title of Figure 1 should be changed to “Experimental design schematic diagram.
(A) In vivo study design. (B) In vitro study design.”
4. Line 133: it should be changed to “Chemicals and Reagents.”
5. Line 193: “PyMol” instead of “Pymol”
6. Line 256: the title of “3.1” is a little bit confused, and please rewrite it.
assistant