Reviewer's report
Title:Constitutive activation of glycogen synthase kinase-3beta correlates with
better prognosis and cyclin-dependent kinase inhibitors in human gastric cancer
Version: 5Date:11 February 2010
Reviewer: Kazunari Tominaga
Reviewer's report:
The authors investigated the relationship between activation of glycogen
synthase kinase-3# and prognosis of gastric cancer and the effect of
cyclin-dependent kinase inhibitors on gastric cancer cells. Overall, the data look
interesting. However, there are some problems with this paper.
Major comment
1. The authors concluded that constitutive activation of GSK correlates with
better prognosis of human gastric cancer by immunohistological analysis.
However, if expression of GSK is constitutive in the stomach and correlated with
the prognosis of patients with gastric cancer after the tumorigenesis, abundant
expression of GSK must be observed in the normal gastric tissues. They should
show the data about the expression of GSK in the normal gastric tissues.
2. The authors did not demonstrate the histological types of the overall cases in
the present study. As they know, gastric cancer cells often exhibit various
biological properties according to their histological types. So, I think this is a
serious problem in this study. They should evaluate the expression of GSK
according to the histological types.
3. In the survival data, there are some differences in the number of each stage of
gastric cancer between GSK-positive and –negative groups. Actually, survival
data about gastric cancer is generally correlated with the ratio of clinical stages.
Thus, I think this significant result about clinical survival may be due to the ratio
of clinical stages but not the differences in the expression of GSK. In addition, the
authors did not show the detailed information about postoperative adjuvant
chemotherapy which is an important factor for the prognosis of gastric cancer.
Level of interest: An article of insufficient interest to warrant publication in a
scientific/medical journal
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Reviewer's report
Title:Constitutive activation of glycogen synthase kinase-3beta correlates with
better prognosis and cyclin-dependent kinase inhibitors in human gastric cancer
Version: 5Date:11 February 2010
Reviewer: Kazunari Tominaga
Reviewer's report:
The authors investigated the relationship between activation of glycogen
synthase kinase-3# and prognosis of gastric cancer and the effect of
cyclin-dependent kinase inhibitors on gastric cancer cells. Overall, the data look
interesting. However, there are some problems with this paper.
Major comment
1. The authors concluded that constitutive activation of GSK correlates with
better prognosis of human gastric cancer by immunohistological analysis.
However, if expression of GSK is constitutive in the stomach and correlated with
the prognosis of patients with gastric cancer after the tumorigenesis, abundant
expression of GSK must be observed in the normal gastric tissues. They should
show the data about the expression of GSK in the normal gastric tissues.
2. The authors did not demonstrate the histological types of the overall cases in
the present study. As they know, gastric cancer cells often exhibit various
biological properties according to their histological types. So, I think this is a
serious problem in this study. They should evaluate the expression of GSK
according to the histological types.
3. In the survival data, there are some differences in the number of each stage of
gastric cancer between GSK-positive and –negative groups. Actually, survival
data about gastric cancer is generally correlated with the ratio of clinical stages.
Thus, I think this significant result about clinical survival may be due to the ratio
of clinical stages but not the differences in the expression of GSK. In addition, the
authors did not show the detailed information about postoperative adjuvant
chemotherapy which is an important factor for the prognosis of gastric cancer.
Level of interest: An article of insufficient interest to warrant publication in a
scientific/medical journal
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Please review this paper.
user
Reviewer's report
Title:Constitutive activation of glycogen synthase kinase-3beta correlates with
better prognosis and cyclin-dependent kinase inhibitors in human gastric cancer
Version: 5Date:11 February 2010
Reviewer: Kazunari Tominaga
Reviewer's report:
The authors investigated the relationship between activation of glycogen
synthase kinase-3# and prognosis of gastric cancer and the effect of
cyclin-dependent kinase inhibitors on gastric cancer cells. Overall, the data look
interesting. However, there are some problems with this paper.
Major comment
1. The authors concluded that constitutive activation of GSK correlates with
better prognosis of human gastric cancer by immunohistological analysis.
However, if expression of GSK is constitutive in the stomach and correlated with
the prognosis of patients with gastric cancer after the tumorigenesis, abundant
expression of GSK must be observed in the normal gastric tissues. They should
show the data about the expression of GSK in the normal gastric tissues.
2. The authors did not demonstrate the histological types of the overall cases in
the present study. As they know, gastric cancer cells often exhibit various
biological properties according to their histological types. So, I think this is a
serious problem in this study. They should evaluate the expression of GSK
according to the histological types.
3. In the survival data, there are some differences in the number of each stage of
gastric cancer between GSK-positive and –negative groups. Actually, survival
data about gastric cancer is generally correlated with the ratio of clinical stages.
Thus, I think this significant result about clinical survival may be due to the ratio
of clinical stages but not the differences in the expression of GSK. In addition, the
authors did not show the detailed information about postoperative adjuvant
chemotherapy which is an important factor for the prognosis of gastric cancer.
Level of interest: An article of insufficient interest to warrant publication in a
scientific/medical journal
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
assistant