Reviewer's report
Title:Supplementation with vitamin E (alpha-tocopherol) in diabetic rats: effects
on the proximal colon
Version: 1Date:1 June 2009
Reviewer: Cheng Juei-Tang
Reviewer's report:
Antioxidant substances as vitamin E seem important in the reduction of the
neurological damages caused by DM. The aim of this study was to evaluate if the
vitamin E (#-tocopherol) in different concentrations perform any effects over the
morphology of the intestinal wall and intrinsic innervation in the proximal colon of
diabetic rats. Rats in diabetic or not were supplemented with vitamin E (0.1%)
and (2%), respectively. Authors found that this supplementation of vitamin E
significantly reduced glycemia and the values of the glycated hemoglobin and
preserved the number of myenteric neurons for animals in diabetic rats, without
effects for the intestine area, thickness of intestinal wall and muscular tunic. It
seems interesting and helpful. But some of the points as follows shall be
concerned.
1. Penis vein was used to inject the streptozotocin (STZ). Is this really? What is
the background you followed? The method in reference 27th did not show this
way.
2. This result is obtained from the reduction of blood sugar or not. Please make
sure of this.
3. Direct effect of vitamin E on neurogeneration in cultured cell will be helpful to
support this finding. Please add the reference(s).
4. The histological pictures in figures shall be improved.
5. What is the difference of this from previous reports such as reference 22 or
26? Please discuss this in detail.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:Supplementation with vitamin E (alpha-tocopherol) in diabetic rats: effects
on the proximal colon
Version: 1Date:1 June 2009
Reviewer: Cheng Juei-Tang
Reviewer's report:
Antioxidant substances as vitamin E seem important in the reduction of the
neurological damages caused by DM. The aim of this study was to evaluate if the
vitamin E (#-tocopherol) in different concentrations perform any effects over the
morphology of the intestinal wall and intrinsic innervation in the proximal colon of
diabetic rats. Rats in diabetic or not were supplemented with vitamin E (0.1%)
and (2%), respectively. Authors found that this supplementation of vitamin E
significantly reduced glycemia and the values of the glycated hemoglobin and
preserved the number of myenteric neurons for animals in diabetic rats, without
effects for the intestine area, thickness of intestinal wall and muscular tunic. It
seems interesting and helpful. But some of the points as follows shall be
concerned.
1. Penis vein was used to inject the streptozotocin (STZ). Is this really? What is
the background you followed? The method in reference 27th did not show this
way.
2. This result is obtained from the reduction of blood sugar or not. Please make
sure of this.
3. Direct effect of vitamin E on neurogeneration in cultured cell will be helpful to
support this finding. Please add the reference(s).
4. The histological pictures in figures shall be improved.
5. What is the difference of this from previous reports such as reference 22 or
26? Please discuss this in detail.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:Supplementation with vitamin E (alpha-tocopherol) in diabetic rats: effects
on the proximal colon
Version: 1Date:1 June 2009
Reviewer: Cheng Juei-Tang
Reviewer's report:
Antioxidant substances as vitamin E seem important in the reduction of the
neurological damages caused by DM. The aim of this study was to evaluate if the
vitamin E (#-tocopherol) in different concentrations perform any effects over the
morphology of the intestinal wall and intrinsic innervation in the proximal colon of
diabetic rats. Rats in diabetic or not were supplemented with vitamin E (0.1%)
and (2%), respectively. Authors found that this supplementation of vitamin E
significantly reduced glycemia and the values of the glycated hemoglobin and
preserved the number of myenteric neurons for animals in diabetic rats, without
effects for the intestine area, thickness of intestinal wall and muscular tunic. It
seems interesting and helpful. But some of the points as follows shall be
concerned.
1. Penis vein was used to inject the streptozotocin (STZ). Is this really? What is
the background you followed? The method in reference 27th did not show this
way.
2. This result is obtained from the reduction of blood sugar or not. Please make
sure of this.
3. Direct effect of vitamin E on neurogeneration in cultured cell will be helpful to
support this finding. Please add the reference(s).
4. The histological pictures in figures shall be improved.
5. What is the difference of this from previous reports such as reference 22 or
26? Please discuss this in detail.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
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