Reviewer's report
Title:Autoimmune enteropathy with a CD8+ CD7- T-cell small bowel
intraepithelial lymphocytosis: case report and literature review
Version: 2Date:17 June 2011
Reviewer: joseph murray
Reviewer's report:
1. Was specific treatment for tropical sprue given, i.e. folic acid and B12, and
what was the patient’s travel history in the months prior to the onset of the
illness?
2. Were T cell gene rearrangements tested?
3. Is it possible this could represent environmental enteropathy or what used to
be called tropical enteropathy, which is endemic in parts of the developing world?
4. Did the patient have any involvement in other organs, such as the stomach?
Was the patient exposed to any irritant medications, such as non-steroidal
anti-inflammatories?
5. Was the intraepithelial lymphocyte clonal – by this, was T cell gene
rearrangement by PCR or flow cytometry undertaken to identify clonal
expansion?
6. What methodology was used for measuring enterocyte antibodies? Was this
an ELISA-based test using extracted antigen or was this indirect
immunofluorescence undertaken on human or monkey intestine? Were goblet
cells diminished or absent?
7. Were any of the CD8 cells positive for markers for cytotoxicity?
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no interests.
Reviewer's report
Title:Autoimmune enteropathy with a CD8+ CD7- T-cell small bowel
intraepithelial lymphocytosis: case report and literature review
Version: 2Date:17 June 2011
Reviewer: joseph murray
Reviewer's report:
1. Was specific treatment for tropical sprue given, i.e. folic acid and B12, and
what was the patient’s travel history in the months prior to the onset of the
illness?
2. Were T cell gene rearrangements tested?
3. Is it possible this could represent environmental enteropathy or what used to
be called tropical enteropathy, which is endemic in parts of the developing world?
4. Did the patient have any involvement in other organs, such as the stomach?
Was the patient exposed to any irritant medications, such as non-steroidal
anti-inflammatories?
5. Was the intraepithelial lymphocyte clonal – by this, was T cell gene
rearrangement by PCR or flow cytometry undertaken to identify clonal
expansion?
6. What methodology was used for measuring enterocyte antibodies? Was this
an ELISA-based test using extracted antigen or was this indirect
immunofluorescence undertaken on human or monkey intestine? Were goblet
cells diminished or absent?
7. Were any of the CD8 cells positive for markers for cytotoxicity?
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no interests.
Please review this paper.
user
Reviewer's report
Title:Autoimmune enteropathy with a CD8+ CD7- T-cell small bowel
intraepithelial lymphocytosis: case report and literature review
Version: 2Date:17 June 2011
Reviewer: joseph murray
Reviewer's report:
1. Was specific treatment for tropical sprue given, i.e. folic acid and B12, and
what was the patient’s travel history in the months prior to the onset of the
illness?
2. Were T cell gene rearrangements tested?
3. Is it possible this could represent environmental enteropathy or what used to
be called tropical enteropathy, which is endemic in parts of the developing world?
4. Did the patient have any involvement in other organs, such as the stomach?
Was the patient exposed to any irritant medications, such as non-steroidal
anti-inflammatories?
5. Was the intraepithelial lymphocyte clonal – by this, was T cell gene
rearrangement by PCR or flow cytometry undertaken to identify clonal
expansion?
6. What methodology was used for measuring enterocyte antibodies? Was this
an ELISA-based test using extracted antigen or was this indirect
immunofluorescence undertaken on human or monkey intestine? Were goblet
cells diminished or absent?
7. Were any of the CD8 cells positive for markers for cytotoxicity?
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no interests.
assistant