Reviewer's report 
 Energy metabolism in pathological human liver Title: 
 2  31 December 2004 Version: Date: 
 Michel Vaubourdolle Reviewer: 
Reviewer's report: 
General 
The authors measured total CK activity and separate CK isoenzymes in 25 samples from liver 
transplants. The work is well-conducted concerning analytical aspects. However, some major points in the 
discussion are not supported by data presented. 
------------------------------------------------------------------------------- Major Compulsory Revisions (that the author must respond to before a decision on publication can be reached) 
1- Liver total homogenate represents the sum of CK activities from hepatocytes and sinusoidal cells, 
which are known to be different regarding to CK isoenzymes content. Thus, it is very difficult to 
interpret results from these isolated data. It is necessary to compare these results with histochemical
data or isolated cells data. 
2- The number of patients is very weak (n=25) with a great heterogeneity. The quantitaive data are 
thus statistically not significant and interpretation of HCC occurence on CK activity cannot be 
supported (n=2) 
3- Discussion section concerning p53 and gene aspects : data presented do not support the 
discussion 
------------------------------------------------------------------------------- 
Minor Essential Revisions (such as missing labels on figures, or the wrong use of a term, which the author can be trusted to correct) 
1- Clinical data from patients are missing 
2- Paper title is not consistent with data presented 
------------------------------------------------------------------------------- 
Discretionary Revisions (which the author can choose to ignore) 
 Unable to decide on acceptance or rejection until the authors have responded to the What next?: 
major compulsory revisions 
 An article whose findings are important to those with closely related research Level of interest: 
interests 
 Not suitable for publication unless extensively edited Quality of written English: 
 Yes Statistical review: 
Declaration of competing interests: 
I declare that I have no competing interests 
Reviewer's report 
 Energy metabolism in pathological human liver Title: 
 2  31 December 2004 Version: Date: 
 Michel Vaubourdolle Reviewer: 
Reviewer's report: 
General 
The authors measured total CK activity and separate CK isoenzymes in 25 samples from liver 
transplants. The work is well-conducted concerning analytical aspects. However, some major points in the 
discussion are not supported by data presented. 
------------------------------------------------------------------------------- Major Compulsory Revisions (that the author must respond to before a decision on publication can be reached) 
1- Liver total homogenate represents the sum of CK activities from hepatocytes and sinusoidal cells, 
which are known to be different regarding to CK isoenzymes content. Thus, it is very difficult to 
interpret results from these isolated data. It is necessary to compare these results with histochemical
data or isolated cells data. 
2- The number of patients is very weak (n=25) with a great heterogeneity. The quantitaive data are 
thus statistically not significant and interpretation of HCC occurence on CK activity cannot be 
supported (n=2) 
3- Discussion section concerning p53 and gene aspects : data presented do not support the 
discussion 
------------------------------------------------------------------------------- 
Minor Essential Revisions (such as missing labels on figures, or the wrong use of a term, which the author can be trusted to correct) 
1- Clinical data from patients are missing 
2- Paper title is not consistent with data presented 
------------------------------------------------------------------------------- 
Discretionary Revisions (which the author can choose to ignore) 
 Unable to decide on acceptance or rejection until the authors have responded to the What next?: 
major compulsory revisions 
 An article whose findings are important to those with closely related research Level of interest: 
interests 
 Not suitable for publication unless extensively edited Quality of written English: 
 Yes Statistical review: 
Declaration of competing interests: 
I declare that I have no competing interests 
Please review this paper.
user
Reviewer's report 
 Energy metabolism in pathological human liver Title: 
 2  31 December 2004 Version: Date: 
 Michel Vaubourdolle Reviewer: 
Reviewer's report: 
General 
The authors measured total CK activity and separate CK isoenzymes in 25 samples from liver 
transplants. The work is well-conducted concerning analytical aspects. However, some major points in the 
discussion are not supported by data presented. 
------------------------------------------------------------------------------- Major Compulsory Revisions (that the author must respond to before a decision on publication can be reached) 
1- Liver total homogenate represents the sum of CK activities from hepatocytes and sinusoidal cells, 
which are known to be different regarding to CK isoenzymes content. Thus, it is very difficult to 
interpret results from these isolated data. It is necessary to compare these results with histochemical
data or isolated cells data. 
2- The number of patients is very weak (n=25) with a great heterogeneity. The quantitaive data are 
thus statistically not significant and interpretation of HCC occurence on CK activity cannot be 
supported (n=2) 
3- Discussion section concerning p53 and gene aspects : data presented do not support the 
discussion 
------------------------------------------------------------------------------- 
Minor Essential Revisions (such as missing labels on figures, or the wrong use of a term, which the author can be trusted to correct) 
1- Clinical data from patients are missing 
2- Paper title is not consistent with data presented 
------------------------------------------------------------------------------- 
Discretionary Revisions (which the author can choose to ignore) 
 Unable to decide on acceptance or rejection until the authors have responded to the What next?: 
major compulsory revisions 
 An article whose findings are important to those with closely related research Level of interest: 
interests 
 Not suitable for publication unless extensively edited Quality of written English: 
 Yes Statistical review: 
Declaration of competing interests: 
I declare that I have no competing interests 
assistant