Reviewer's report
Title:Prevalence of Helicobacter pylori high tetracycline resistant 16S rDNA
AGA926-928TTC genotype in gastric biopsy specimens from dyspeptic patients
of a city in the interior of Sao Paulo, Brazil
Version: 1Date:31 January 2012
Reviewer: Francis Megraud
Reviewer's report:
In this manuscript R.B. Suzuki et al. report their experience in the detection of H.
pylori and H. pylori resistance to tetracycline using a PCR on the 16S rRNA gene
followed by RFLP with the HinfI restriction enzyme.
They detected H. pylori by PCR in 395/435 (90.8%) patients for which histology
was positive. Restriction of the 545 bp amplicon was obtained in 393 out of the
395 cases and presented the tetracycline susceptible pattern. They found that
the absence of restriction of the 2 other amplicons was due to a substitution at
position 958.
An interesting result of this study, performed on a large number of cases, is the
absence of the tetracycline resistance pattern of H. pylori in a region where it was
claimed that this pattern was relatively frequent.
A limit of the study is the lack of culture which would have increased the
accuracy of the H. pylori diagnosis and would have allowed a verification of
whether another mechanism of tetracycline resistance was present.
The article could be shorter especially the background. It would be good to briefly
explain that the amplicon restriction leads to 3 bands instead of 2 when the
resistance genotype is present.
The title should be: “Absence of Helicobacter pylori high tetracycline resistant….”
(instead of Prevalence).
There are a number of misspellings to be corrected.
Page 10, line 13: the reference to Ribeiro is No. 42.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
No.
Reviewer's report
Title:Prevalence of Helicobacter pylori high tetracycline resistant 16S rDNA
AGA926-928TTC genotype in gastric biopsy specimens from dyspeptic patients
of a city in the interior of Sao Paulo, Brazil
Version: 1Date:31 January 2012
Reviewer: Francis Megraud
Reviewer's report:
In this manuscript R.B. Suzuki et al. report their experience in the detection of H.
pylori and H. pylori resistance to tetracycline using a PCR on the 16S rRNA gene
followed by RFLP with the HinfI restriction enzyme.
They detected H. pylori by PCR in 395/435 (90.8%) patients for which histology
was positive. Restriction of the 545 bp amplicon was obtained in 393 out of the
395 cases and presented the tetracycline susceptible pattern. They found that
the absence of restriction of the 2 other amplicons was due to a substitution at
position 958.
An interesting result of this study, performed on a large number of cases, is the
absence of the tetracycline resistance pattern of H. pylori in a region where it was
claimed that this pattern was relatively frequent.
A limit of the study is the lack of culture which would have increased the
accuracy of the H. pylori diagnosis and would have allowed a verification of
whether another mechanism of tetracycline resistance was present.
The article could be shorter especially the background. It would be good to briefly
explain that the amplicon restriction leads to 3 bands instead of 2 when the
resistance genotype is present.
The title should be: “Absence of Helicobacter pylori high tetracycline resistant….”
(instead of Prevalence).
There are a number of misspellings to be corrected.
Page 10, line 13: the reference to Ribeiro is No. 42.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
No.
Please review this paper.
user
Reviewer's report
Title:Prevalence of Helicobacter pylori high tetracycline resistant 16S rDNA
AGA926-928TTC genotype in gastric biopsy specimens from dyspeptic patients
of a city in the interior of Sao Paulo, Brazil
Version: 1Date:31 January 2012
Reviewer: Francis Megraud
Reviewer's report:
In this manuscript R.B. Suzuki et al. report their experience in the detection of H.
pylori and H. pylori resistance to tetracycline using a PCR on the 16S rRNA gene
followed by RFLP with the HinfI restriction enzyme.
They detected H. pylori by PCR in 395/435 (90.8%) patients for which histology
was positive. Restriction of the 545 bp amplicon was obtained in 393 out of the
395 cases and presented the tetracycline susceptible pattern. They found that
the absence of restriction of the 2 other amplicons was due to a substitution at
position 958.
An interesting result of this study, performed on a large number of cases, is the
absence of the tetracycline resistance pattern of H. pylori in a region where it was
claimed that this pattern was relatively frequent.
A limit of the study is the lack of culture which would have increased the
accuracy of the H. pylori diagnosis and would have allowed a verification of
whether another mechanism of tetracycline resistance was present.
The article could be shorter especially the background. It would be good to briefly
explain that the amplicon restriction leads to 3 bands instead of 2 when the
resistance genotype is present.
The title should be: “Absence of Helicobacter pylori high tetracycline resistant….”
(instead of Prevalence).
There are a number of misspellings to be corrected.
Page 10, line 13: the reference to Ribeiro is No. 42.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
No.
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