Title: Intestinal Claudin-7 deficiency impacts the intestinal microbiota in mice with colitis
Version: 0 Date: 24 Apr 2021
Reviewer's report:
This manuscript investigated the role of claudin-7 in intestinal damage repair. The topic is
relevant, as claudin-7 has been reported to be decreased in lBD, and claudin-7 ko has been
demonstrated to cause decreased intestinal stem cell function and intestinal inflammation.
However, the current study is preliminary and descriptive.
Specific comments
1. Animal naming system and shortened is very confusing and rather disruptive for the reviewer
to understand the manuscript. They do not follow conventional mutant mice naming system
and in the microbiome part of the manuscript, an even confusing shortened names are used to
indicate the already confusing name. Description of animals is inadequate, it is not clear what
exactly mice are used for test and control. Although VilCreERT2 transgenic mice are used,
no description of tamoxifen injection is described. In addition, Dr. Sylvie Robine is a female
professor.
2. Rather than a good model for IBD, DSS treatment is a better model for intestinal epithelial
damage. The description should be revised accordingly throughout the manuscript.
3. Fig. 1-2, when possible, individual data points should be shown in addition to average and SE.
4. Fig. 1E. The section of colon shown is not clear, histology also need quantification.
5. Characterization of the colon phenotype, including proliferation cell number, apoptotic cell
number, epithelial cell migration, cytokine production, immune cell number, and immune cell
population remain to be quantified.
6. Fig 2A-C. The author claimed that there is decreased Muc2 producing goblets in claudin-7
KO mice, however, this is not a fair comparison, as claudin-7 KO mice treated with DSS have no
epithelium left, thus the loss of Muc2 producing cell may simply caused by loss of all epithelial
cells. The fraction of Muc2 producing goblet cells of all epithelial cells should be measured.
7. Fig 2D. The images shown in D does not match quantification shown in E. What is the reason
for this?
8. Description of microbiome studies is inadequate. Only methods for alfa and beta diversity
calculation are described, while a lot more analyses were done.
9. Fig. 3-4. Fonts in figure panels are too small to see.
10. Although some differences can be seen, the contribution of altered microbiome to DSS
induced damage was not determined. How dose reducing bacterial load affect DSS induced
tissue damage?
Title: Intestinal Claudin-7 deficiency impacts the intestinal microbiota in mice with colitis
Version: 0 Date: 24 Apr 2021
Reviewer's report:
This manuscript investigated the role of claudin-7 in intestinal damage repair. The topic is
relevant, as claudin-7 has been reported to be decreased in lBD, and claudin-7 ko has been
demonstrated to cause decreased intestinal stem cell function and intestinal inflammation.
However, the current study is preliminary and descriptive.
Specific comments
1. Animal naming system and shortened is very confusing and rather disruptive for the reviewer
to understand the manuscript. They do not follow conventional mutant mice naming system
and in the microbiome part of the manuscript, an even confusing shortened names are used to
indicate the already confusing name. Description of animals is inadequate, it is not clear what
exactly mice are used for test and control. Although VilCreERT2 transgenic mice are used,
no description of tamoxifen injection is described. In addition, Dr. Sylvie Robine is a female
professor.
2. Rather than a good model for IBD, DSS treatment is a better model for intestinal epithelial
damage. The description should be revised accordingly throughout the manuscript.
3. Fig. 1-2, when possible, individual data points should be shown in addition to average and SE.
4. Fig. 1E. The section of colon shown is not clear, histology also need quantification.
5. Characterization of the colon phenotype, including proliferation cell number, apoptotic cell
number, epithelial cell migration, cytokine production, immune cell number, and immune cell
population remain to be quantified.
6. Fig 2A-C. The author claimed that there is decreased Muc2 producing goblets in claudin-7
KO mice, however, this is not a fair comparison, as claudin-7 KO mice treated with DSS have no
epithelium left, thus the loss of Muc2 producing cell may simply caused by loss of all epithelial
cells. The fraction of Muc2 producing goblet cells of all epithelial cells should be measured.
7. Fig 2D. The images shown in D does not match quantification shown in E. What is the reason
for this?
8. Description of microbiome studies is inadequate. Only methods for alfa and beta diversity
calculation are described, while a lot more analyses were done.
9. Fig. 3-4. Fonts in figure panels are too small to see.
10. Although some differences can be seen, the contribution of altered microbiome to DSS
induced damage was not determined. How dose reducing bacterial load affect DSS induced
tissue damage?
Please review this paper.
user
Title: Intestinal Claudin-7 deficiency impacts the intestinal microbiota in mice with colitis
Version: 0 Date: 24 Apr 2021
Reviewer's report:
This manuscript investigated the role of claudin-7 in intestinal damage repair. The topic is
relevant, as claudin-7 has been reported to be decreased in lBD, and claudin-7 ko has been
demonstrated to cause decreased intestinal stem cell function and intestinal inflammation.
However, the current study is preliminary and descriptive.
Specific comments
1. Animal naming system and shortened is very confusing and rather disruptive for the reviewer
to understand the manuscript. They do not follow conventional mutant mice naming system
and in the microbiome part of the manuscript, an even confusing shortened names are used to
indicate the already confusing name. Description of animals is inadequate, it is not clear what
exactly mice are used for test and control. Although VilCreERT2 transgenic mice are used,
no description of tamoxifen injection is described. In addition, Dr. Sylvie Robine is a female
professor.
2. Rather than a good model for IBD, DSS treatment is a better model for intestinal epithelial
damage. The description should be revised accordingly throughout the manuscript.
3. Fig. 1-2, when possible, individual data points should be shown in addition to average and SE.
4. Fig. 1E. The section of colon shown is not clear, histology also need quantification.
5. Characterization of the colon phenotype, including proliferation cell number, apoptotic cell
number, epithelial cell migration, cytokine production, immune cell number, and immune cell
population remain to be quantified.
6. Fig 2A-C. The author claimed that there is decreased Muc2 producing goblets in claudin-7
KO mice, however, this is not a fair comparison, as claudin-7 KO mice treated with DSS have no
epithelium left, thus the loss of Muc2 producing cell may simply caused by loss of all epithelial
cells. The fraction of Muc2 producing goblet cells of all epithelial cells should be measured.
7. Fig 2D. The images shown in D does not match quantification shown in E. What is the reason
for this?
8. Description of microbiome studies is inadequate. Only methods for alfa and beta diversity
calculation are described, while a lot more analyses were done.
9. Fig. 3-4. Fonts in figure panels are too small to see.
10. Although some differences can be seen, the contribution of altered microbiome to DSS
induced damage was not determined. How dose reducing bacterial load affect DSS induced
tissue damage?
assistant