Reviewer's report
Title:Overexpression of SLC38A1 is associated with poorer prognosis in
Chinese patients with gastric cancer
Version: 1Date:12 August 2013
Reviewer: Rui Manuel Reis
Reviewer's report:
Overexpression of SLC38A1 is associated with poorer prognosis in Chinese
patients with gastric cancer
Jing Xie1*#, M.D., Jie-jun Wang*3, M.D., Ping Li2#, MS, Hui-feng Gao1, MS,
Jian-Xin Qian3, MS, Ling-Yan Yuan3, MS
In the present study, Jing Xie and co-authors analyzed the IHC profile of large
series of gastric cancer, and correlated with patient’s clinical-pathological
features. The authors find that SLC38A1 overexpression is an independent
prognostic factor and in vitro depletion of SLC38A1 was associated with a lower
aggressive behavior.
The study is interesting, pertinent and the overall results are important for the
scientific community. Despite of that, some issues most be answered in order to
send a rigorous and clear message of this manuscript.
Major Compulsory Revisions
- Despite the mention that demographic and clinical-pathological features of the
series herein analyzed were previously described ion other studies, it is
fundamental to show to the readers of the present study that epidemiological
data in the format of a new table 1.
- The manuscript lacks current table 1 and table 2, and without that it is
impossible to assess the text written by the authors.
- Considering the IHC analysis. The authors correctly used as a negative control,
the omission of the primary antibody. However, it is also mandatory to use a
second negative control, where the primary antibody is replaced by a universal
negative antibody from the specie, in order to evaluate the specificity of the
SLC38A1 antibody used in the study.
- In the material and methods, or results section, the authors most detailed the
semi-quantitative scoring system used for IHC evaluation.
- In the results section, a more detailed and deeper description of the SLC38A1
expression pattern must be presented. What types of cells were positive, it was
focal, diffuse, etc.
- The in vitro results are interesting, however, it is missing the behavior of the
parental cells in all the experiments.
Minor Essential Revisions
Some grammatical mistakes should be corrected. A full written re-evaluation is
suggested.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Overexpression of SLC38A1 is associated with poorer prognosis in
Chinese patients with gastric cancer
Version: 1Date:12 August 2013
Reviewer: Rui Manuel Reis
Reviewer's report:
Overexpression of SLC38A1 is associated with poorer prognosis in Chinese
patients with gastric cancer
Jing Xie1*#, M.D., Jie-jun Wang*3, M.D., Ping Li2#, MS, Hui-feng Gao1, MS,
Jian-Xin Qian3, MS, Ling-Yan Yuan3, MS
In the present study, Jing Xie and co-authors analyzed the IHC profile of large
series of gastric cancer, and correlated with patient’s clinical-pathological
features. The authors find that SLC38A1 overexpression is an independent
prognostic factor and in vitro depletion of SLC38A1 was associated with a lower
aggressive behavior.
The study is interesting, pertinent and the overall results are important for the
scientific community. Despite of that, some issues most be answered in order to
send a rigorous and clear message of this manuscript.
Major Compulsory Revisions
- Despite the mention that demographic and clinical-pathological features of the
series herein analyzed were previously described ion other studies, it is
fundamental to show to the readers of the present study that epidemiological
data in the format of a new table 1.
- The manuscript lacks current table 1 and table 2, and without that it is
impossible to assess the text written by the authors.
- Considering the IHC analysis. The authors correctly used as a negative control,
the omission of the primary antibody. However, it is also mandatory to use a
second negative control, where the primary antibody is replaced by a universal
negative antibody from the specie, in order to evaluate the specificity of the
SLC38A1 antibody used in the study.
- In the material and methods, or results section, the authors most detailed the
semi-quantitative scoring system used for IHC evaluation.
- In the results section, a more detailed and deeper description of the SLC38A1
expression pattern must be presented. What types of cells were positive, it was
focal, diffuse, etc.
- The in vitro results are interesting, however, it is missing the behavior of the
parental cells in all the experiments.
Minor Essential Revisions
Some grammatical mistakes should be corrected. A full written re-evaluation is
suggested.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Overexpression of SLC38A1 is associated with poorer prognosis in
Chinese patients with gastric cancer
Version: 1Date:12 August 2013
Reviewer: Rui Manuel Reis
Reviewer's report:
Overexpression of SLC38A1 is associated with poorer prognosis in Chinese
patients with gastric cancer
Jing Xie1*#, M.D., Jie-jun Wang*3, M.D., Ping Li2#, MS, Hui-feng Gao1, MS,
Jian-Xin Qian3, MS, Ling-Yan Yuan3, MS
In the present study, Jing Xie and co-authors analyzed the IHC profile of large
series of gastric cancer, and correlated with patient’s clinical-pathological
features. The authors find that SLC38A1 overexpression is an independent
prognostic factor and in vitro depletion of SLC38A1 was associated with a lower
aggressive behavior.
The study is interesting, pertinent and the overall results are important for the
scientific community. Despite of that, some issues most be answered in order to
send a rigorous and clear message of this manuscript.
Major Compulsory Revisions
- Despite the mention that demographic and clinical-pathological features of the
series herein analyzed were previously described ion other studies, it is
fundamental to show to the readers of the present study that epidemiological
data in the format of a new table 1.
- The manuscript lacks current table 1 and table 2, and without that it is
impossible to assess the text written by the authors.
- Considering the IHC analysis. The authors correctly used as a negative control,
the omission of the primary antibody. However, it is also mandatory to use a
second negative control, where the primary antibody is replaced by a universal
negative antibody from the specie, in order to evaluate the specificity of the
SLC38A1 antibody used in the study.
- In the material and methods, or results section, the authors most detailed the
semi-quantitative scoring system used for IHC evaluation.
- In the results section, a more detailed and deeper description of the SLC38A1
expression pattern must be presented. What types of cells were positive, it was
focal, diffuse, etc.
- The in vitro results are interesting, however, it is missing the behavior of the
parental cells in all the experiments.
Minor Essential Revisions
Some grammatical mistakes should be corrected. A full written re-evaluation is
suggested.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
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