Title: Sodium nitroprusside protects HFD induced gut dysfunction via activating AMPK#/
SIRT1 signaling
Version: 5 Date: 07 May 2021
Reviewer's report:
The manuscript has been revised as suggested previously, but it still needs revision prior to
publication. Besides, the language need to be further improved, there are still many typos and
grammatical errors, it is unacceptable at its current form, please seek for help from native English
speakers or professionals.
Line 31 mechnism---mechanism
Line 30 Please delete the extra space between “to” and “activate”, similar modifications should
be done throughout the text (i.e. line 54, 61 and 77).
Line 42 Please add space between “complicated” and “by”, same mistakes can be found at line
51, 85 and 230 etc. Please check again throughout the manuscript.
Line 44, implied-imply, alleviate-alleviates
Line 101 Please insert space between the numeral and the unit, i.e. 1mg/mL should be changed
to 1 mg/mL.  The only two exceptions are % and #.  Similar modifications should be done
throughout the text, including all figures.
Line 120 “mg/ml” should be changed to “mg/mL”, please keep consistent with the former.
Similar modifications should be done throughout the text (i.e. line 135-136, ng/mL instead of
ng/ml).
Line 131 The procedure for cell culture is too simple, lacking some key information, such as
the density of cells, types of plates, cell passage number and cell culture condition, etc. Please
add the above and other necessary information.
Line 196, 207, 236, please replace “compared to” by “compared with”, as the phrase compare
to is used when the intent is to assert, without the need for elaboration, that two or more items
are similar. Whereas the phrase compare with is used to juxtapose two or more items to illustrate
similarities and/or differences.
Line 257 Please replace the word “involed” by “involved” and add “be” before it.
Fig 1 H K I, Please revise the word “exprssion” in the ordinate title.
Furthermore, the resolution of the figures need to be improved, especially Fig 4C and Fig 5A
and B
Title: Sodium nitroprusside protects HFD induced gut dysfunction via activating AMPK#/
SIRT1 signaling
Version: 5 Date: 07 May 2021
Reviewer's report:
The manuscript has been revised as suggested previously, but it still needs revision prior to
publication. Besides, the language need to be further improved, there are still many typos and
grammatical errors, it is unacceptable at its current form, please seek for help from native English
speakers or professionals.
Line 31 mechnism---mechanism
Line 30 Please delete the extra space between “to” and “activate”, similar modifications should
be done throughout the text (i.e. line 54, 61 and 77).
Line 42 Please add space between “complicated” and “by”, same mistakes can be found at line
51, 85 and 230 etc. Please check again throughout the manuscript.
Line 44, implied-imply, alleviate-alleviates
Line 101 Please insert space between the numeral and the unit, i.e. 1mg/mL should be changed
to 1 mg/mL.  The only two exceptions are % and #.  Similar modifications should be done
throughout the text, including all figures.
Line 120 “mg/ml” should be changed to “mg/mL”, please keep consistent with the former.
Similar modifications should be done throughout the text (i.e. line 135-136, ng/mL instead of
ng/ml).
Line 131 The procedure for cell culture is too simple, lacking some key information, such as
the density of cells, types of plates, cell passage number and cell culture condition, etc. Please
add the above and other necessary information.
Line 196, 207, 236, please replace “compared to” by “compared with”, as the phrase compare
to is used when the intent is to assert, without the need for elaboration, that two or more items
are similar. Whereas the phrase compare with is used to juxtapose two or more items to illustrate
similarities and/or differences.
Line 257 Please replace the word “involed” by “involved” and add “be” before it.
Fig 1 H K I, Please revise the word “exprssion” in the ordinate title.
Furthermore, the resolution of the figures need to be improved, especially Fig 4C and Fig 5A
and B
Please review this paper.
user
Title: Sodium nitroprusside protects HFD induced gut dysfunction via activating AMPK#/
SIRT1 signaling
Version: 5 Date: 07 May 2021
Reviewer's report:
The manuscript has been revised as suggested previously, but it still needs revision prior to
publication. Besides, the language need to be further improved, there are still many typos and
grammatical errors, it is unacceptable at its current form, please seek for help from native English
speakers or professionals.
Line 31 mechnism---mechanism
Line 30 Please delete the extra space between “to” and “activate”, similar modifications should
be done throughout the text (i.e. line 54, 61 and 77).
Line 42 Please add space between “complicated” and “by”, same mistakes can be found at line
51, 85 and 230 etc. Please check again throughout the manuscript.
Line 44, implied-imply, alleviate-alleviates
Line 101 Please insert space between the numeral and the unit, i.e. 1mg/mL should be changed
to 1 mg/mL.  The only two exceptions are % and #.  Similar modifications should be done
throughout the text, including all figures.
Line 120 “mg/ml” should be changed to “mg/mL”, please keep consistent with the former.
Similar modifications should be done throughout the text (i.e. line 135-136, ng/mL instead of
ng/ml).
Line 131 The procedure for cell culture is too simple, lacking some key information, such as
the density of cells, types of plates, cell passage number and cell culture condition, etc. Please
add the above and other necessary information.
Line 196, 207, 236, please replace “compared to” by “compared with”, as the phrase compare
to is used when the intent is to assert, without the need for elaboration, that two or more items
are similar. Whereas the phrase compare with is used to juxtapose two or more items to illustrate
similarities and/or differences.
Line 257 Please replace the word “involed” by “involved” and add “be” before it.
Fig 1 H K I, Please revise the word “exprssion” in the ordinate title.
Furthermore, the resolution of the figures need to be improved, especially Fig 4C and Fig 5A
and B
assistant