Reviewer's report
Title:NOX2-generated oxidative stress is associated with severity of ultrasound
liver steatosis in patients with non-alcoholic fatty liver disease
Version:3Date:16 December 2013
Reviewer: Carmine Finelli
Reviewer's report:
Major Compulsory Revisions
• What is the concentration of 8-iso-prostaglandin F2# (8-iso-PGF2#) in patients
with NAFLD without METS?
• Are patients smoking?
• What is the renal function of patients?!
In fact, the prostaglandin, 8-iso-PGF2#, is an isoprostane that is produced by the
non-enzymatic peroxidation of arachidonic acid in membrane phospholipids.
Measurement of 8-iso-PGF2# is a reliable tool for the identification of subjects
with enhanced rates of lipid peroxidation. Enhanced formation of 8-iso- PGF2#
has been reported in association with several cardiovascu¬lar risk factors, as
well as atherosclerosis. Cigarette smoking is a risk factor for renal function
alteration in healthy smokers and is characterized by a high eGFR and a high
urinary protein associated with an increase in the 8-iso- PGF2# and hs-CRP.
(Sauriasari R, Sakano N, Wang DH, Takaki J, Takemoto K, Wang B, Sugiyama
H, Sato Y, Takigawa T, Takahashi N, Kanbara S, Hitomi Y, Nakamura H, Ogino
K. C-reactive protein is associated with cigarette smoking-induced hyperfiltration
and proteinuria in an apparently healthy population. Hypertens Res. 2010 Nov;
33 (11): 1129-36.)
Patients with NAFLD have almost all damage endothelial (dysfunction). This
damage is known to be correlated with elevated concentrations of 8-iso-PGF2#
(Nassar H, Furukado S, Tanaka M, Miwa K, Okazaki S, Sakaguchi M, Mochizuki
H, Kitagawa K. The relation between carotid plaque echogenicity and oxidative
stress marker 8-iso-prostaglandin F2#. Ultrasound Med Biol. 2012
Mar;38(3):487-91.). Should it be evaluated perhaps?!
In addition, lacking a healthy control group !!!
Minor Essential Revisions
The interpretation (discussion and conclusion) should be reevaluated in the light
of these results, as well as any statistical analysis.
Tables are not easily accessible to the reader and should be semplicate and
divided.
Discretionary Revisions
• Why the authors not describe patients without METS?
Level of interest: An article of outstanding merit and interest in its field
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:NOX2-generated oxidative stress is associated with severity of ultrasound
liver steatosis in patients with non-alcoholic fatty liver disease
Version:3Date:16 December 2013
Reviewer: Carmine Finelli
Reviewer's report:
Major Compulsory Revisions
• What is the concentration of 8-iso-prostaglandin F2# (8-iso-PGF2#) in patients
with NAFLD without METS?
• Are patients smoking?
• What is the renal function of patients?!
In fact, the prostaglandin, 8-iso-PGF2#, is an isoprostane that is produced by the
non-enzymatic peroxidation of arachidonic acid in membrane phospholipids.
Measurement of 8-iso-PGF2# is a reliable tool for the identification of subjects
with enhanced rates of lipid peroxidation. Enhanced formation of 8-iso- PGF2#
has been reported in association with several cardiovascu¬lar risk factors, as
well as atherosclerosis. Cigarette smoking is a risk factor for renal function
alteration in healthy smokers and is characterized by a high eGFR and a high
urinary protein associated with an increase in the 8-iso- PGF2# and hs-CRP.
(Sauriasari R, Sakano N, Wang DH, Takaki J, Takemoto K, Wang B, Sugiyama
H, Sato Y, Takigawa T, Takahashi N, Kanbara S, Hitomi Y, Nakamura H, Ogino
K. C-reactive protein is associated with cigarette smoking-induced hyperfiltration
and proteinuria in an apparently healthy population. Hypertens Res. 2010 Nov;
33 (11): 1129-36.)
Patients with NAFLD have almost all damage endothelial (dysfunction). This
damage is known to be correlated with elevated concentrations of 8-iso-PGF2#
(Nassar H, Furukado S, Tanaka M, Miwa K, Okazaki S, Sakaguchi M, Mochizuki
H, Kitagawa K. The relation between carotid plaque echogenicity and oxidative
stress marker 8-iso-prostaglandin F2#. Ultrasound Med Biol. 2012
Mar;38(3):487-91.). Should it be evaluated perhaps?!
In addition, lacking a healthy control group !!!
Minor Essential Revisions
The interpretation (discussion and conclusion) should be reevaluated in the light
of these results, as well as any statistical analysis.
Tables are not easily accessible to the reader and should be semplicate and
divided.
Discretionary Revisions
• Why the authors not describe patients without METS?
Level of interest: An article of outstanding merit and interest in its field
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:NOX2-generated oxidative stress is associated with severity of ultrasound
liver steatosis in patients with non-alcoholic fatty liver disease
Version:3Date:16 December 2013
Reviewer: Carmine Finelli
Reviewer's report:
Major Compulsory Revisions
• What is the concentration of 8-iso-prostaglandin F2# (8-iso-PGF2#) in patients
with NAFLD without METS?
• Are patients smoking?
• What is the renal function of patients?!
In fact, the prostaglandin, 8-iso-PGF2#, is an isoprostane that is produced by the
non-enzymatic peroxidation of arachidonic acid in membrane phospholipids.
Measurement of 8-iso-PGF2# is a reliable tool for the identification of subjects
with enhanced rates of lipid peroxidation. Enhanced formation of 8-iso- PGF2#
has been reported in association with several cardiovascu¬lar risk factors, as
well as atherosclerosis. Cigarette smoking is a risk factor for renal function
alteration in healthy smokers and is characterized by a high eGFR and a high
urinary protein associated with an increase in the 8-iso- PGF2# and hs-CRP.
(Sauriasari R, Sakano N, Wang DH, Takaki J, Takemoto K, Wang B, Sugiyama
H, Sato Y, Takigawa T, Takahashi N, Kanbara S, Hitomi Y, Nakamura H, Ogino
K. C-reactive protein is associated with cigarette smoking-induced hyperfiltration
and proteinuria in an apparently healthy population. Hypertens Res. 2010 Nov;
33 (11): 1129-36.)
Patients with NAFLD have almost all damage endothelial (dysfunction). This
damage is known to be correlated with elevated concentrations of 8-iso-PGF2#
(Nassar H, Furukado S, Tanaka M, Miwa K, Okazaki S, Sakaguchi M, Mochizuki
H, Kitagawa K. The relation between carotid plaque echogenicity and oxidative
stress marker 8-iso-prostaglandin F2#. Ultrasound Med Biol. 2012
Mar;38(3):487-91.). Should it be evaluated perhaps?!
In addition, lacking a healthy control group !!!
Minor Essential Revisions
The interpretation (discussion and conclusion) should be reevaluated in the light
of these results, as well as any statistical analysis.
Tables are not easily accessible to the reader and should be semplicate and
divided.
Discretionary Revisions
• Why the authors not describe patients without METS?
Level of interest: An article of outstanding merit and interest in its field
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
Declaration of competing interests:
I declare that I have no competing interests.
assistant