Reviewer’s report  
 
Title: Impaired Liver Regeneration is associated with Reduced Cyclin B1 in Natural Killer T 
cell-deficient mice  
 
Version: 0 Date: 22 Dec 2016  
 
Reviewer: Jana Pindjakova  
 
Reviewer's report:  
 
The authors of the paper are focused on the rol e of natural killer T cells in liver regeneration 
using mouse model of partial hepatectomy in combination with ConA -induced acute hepatitis. 
The model is widely used in studies of liver diseases and many papers on this topic have been 
published.  
 
Comments:  
Introduction:  
- Abbreviation and explanation of used abbreviation are missing: partial hepatectomy (PH) 
page 4 line 31, PCNA page 5 line 17  
- The relationship of cyclin B1 to liver injury and regeneration should be described more or is 
it a typing error on pa ge 5 line 17 (Cyclin D1 or B1?)  
- There is nothing mentioned about IL -6 cytokine and its role in liver diseases  
 
Methods:  
Partial hepatectomy - this part needs revision, it is hard to understand what it means: ...by 
removal of the median and left lateral lob es. 4 days before mice were sacrificed after 3h or 48h.  
 
Results:  
- They are clearly showing that the presence of NKT cells affect liver regeneration in used 
mouse model but the authors should be avoided to use the term 'type I NKT cells' if there is 
no pro of for particular type of NKT cells in the paper - page 8 line 43  
- ELISA results for IL -6 in serum should be supported by detection of gene expression for IL -
6 in injured liver to prove the source of IL -6 in blood  
 
Are the methods appropriate and well descr ibed?  
If not, please specify what is required in your comments to the authors.  
 
No 
 
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
 
Yes 
 
Are the conclusions drawn adequately  supported by the data shown?  
If not, please explain in your comments to the authors.  
 
Yes 
 
Are you able to assess any statistics in the manuscript or would you recommend an 
additional statistical review?  
If an additional statistical review is recommended,  please specify what aspects require further 
assessment in your comments to the editors.  
 
I am able to assess the statistics  
 
Quality of written English  
Please indicate the quality of language in the manuscript:  
 
Acceptable  
 
Declaration of competing intere sts 
Please complete a declaration of competing interests, considering the following questions:  
 
1. Have you in the past five years received reimbursements, fees, funding, or salary from an 
organisation that may in any way gain or lose financially from the pub lication of this 
manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares in an organisation that may in any way gain or lose 
financially from the publication of this manuscript, either now or in the future?  
 
3. Do you hold or are you curren tly applying for any patents relating to the content of the 
manuscript?  
 
4. Have you received reimbursements, fees, funding, or salary from an organization that 
holds or has applied for patents relating to the content of the manuscript?  
 
5. Do you have any other  financial competing interests?  
 
6. Do you have any non -financial competing interests in relation to this paper?  
 
If you can answer no to all of the above, write 'I declare that I have no competing interests' 
below. If your reply is yes to any, please give de tails below.  
 
I declare that I have no competing interests  
 
I agree to the open peer review policy of the journal. I understand that my name will be included 
on my report to the authors and, if the manuscript is accepted for publication, my named report 
including any attachments I upload will be posted on the website along with the authors' 
responses. I agree for my report to be made available under an Open Access Creative Commons  
CC-BY license ( http://creativecommons.org/licenses/by/4.0/ ). I understand th at any comments 
which I do not wish to be included in my named report can be included as confidential comments 
to the editors, which will not be published.  
 
I agree to the open peer review policy of the journal  
Reviewer’s report  
 
Title: Impaired Liver Regeneration is associated with Reduced Cyclin B1 in Natural Killer T 
cell-deficient mice  
 
Version: 0 Date: 22 Dec 2016  
 
Reviewer: Jana Pindjakova  
 
Reviewer's report:  
 
The authors of the paper are focused on the rol e of natural killer T cells in liver regeneration 
using mouse model of partial hepatectomy in combination with ConA -induced acute hepatitis. 
The model is widely used in studies of liver diseases and many papers on this topic have been 
published.  
 
Comments:  
Introduction:  
- Abbreviation and explanation of used abbreviation are missing: partial hepatectomy (PH) 
page 4 line 31, PCNA page 5 line 17  
- The relationship of cyclin B1 to liver injury and regeneration should be described more or is 
it a typing error on pa ge 5 line 17 (Cyclin D1 or B1?)  
- There is nothing mentioned about IL -6 cytokine and its role in liver diseases  
 
Methods:  
Partial hepatectomy - this part needs revision, it is hard to understand what it means: ...by 
removal of the median and left lateral lob es. 4 days before mice were sacrificed after 3h or 48h.  
 
Results:  
- They are clearly showing that the presence of NKT cells affect liver regeneration in used 
mouse model but the authors should be avoided to use the term 'type I NKT cells' if there is 
no pro of for particular type of NKT cells in the paper - page 8 line 43  
- ELISA results for IL -6 in serum should be supported by detection of gene expression for IL -
6 in injured liver to prove the source of IL -6 in blood  
 
Are the methods appropriate and well descr ibed?  
If not, please specify what is required in your comments to the authors.  
 
No 
 
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
 
Yes 
 
Are the conclusions drawn adequately  supported by the data shown?  
If not, please explain in your comments to the authors.  
 
Yes 
 
Are you able to assess any statistics in the manuscript or would you recommend an 
additional statistical review?  
If an additional statistical review is recommended,  please specify what aspects require further 
assessment in your comments to the editors.  
 
I am able to assess the statistics  
 
Quality of written English  
Please indicate the quality of language in the manuscript:  
 
Acceptable  
 
Declaration of competing intere sts 
Please complete a declaration of competing interests, considering the following questions:  
 
1. Have you in the past five years received reimbursements, fees, funding, or salary from an 
organisation that may in any way gain or lose financially from the pub lication of this 
manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares in an organisation that may in any way gain or lose 
financially from the publication of this manuscript, either now or in the future?  
 
3. Do you hold or are you curren tly applying for any patents relating to the content of the 
manuscript?  
 
4. Have you received reimbursements, fees, funding, or salary from an organization that 
holds or has applied for patents relating to the content of the manuscript?  
 
5. Do you have any other  financial competing interests?  
 
6. Do you have any non -financial competing interests in relation to this paper?  
 
If you can answer no to all of the above, write 'I declare that I have no competing interests' 
below. If your reply is yes to any, please give de tails below.  
 
I declare that I have no competing interests  
 
I agree to the open peer review policy of the journal. I understand that my name will be included 
on my report to the authors and, if the manuscript is accepted for publication, my named report 
including any attachments I upload will be posted on the website along with the authors' 
responses. I agree for my report to be made available under an Open Access Creative Commons  
CC-BY license ( http://creativecommons.org/licenses/by/4.0/ ). I understand th at any comments 
which I do not wish to be included in my named report can be included as confidential comments 
to the editors, which will not be published.  
 
I agree to the open peer review policy of the journal  
Please review this paper.
user
Reviewer’s report  
 
Title: Impaired Liver Regeneration is associated with Reduced Cyclin B1 in Natural Killer T 
cell-deficient mice  
 
Version: 0 Date: 22 Dec 2016  
 
Reviewer: Jana Pindjakova  
 
Reviewer's report:  
 
The authors of the paper are focused on the rol e of natural killer T cells in liver regeneration 
using mouse model of partial hepatectomy in combination with ConA -induced acute hepatitis. 
The model is widely used in studies of liver diseases and many papers on this topic have been 
published.  
 
Comments:  
Introduction:  
- Abbreviation and explanation of used abbreviation are missing: partial hepatectomy (PH) 
page 4 line 31, PCNA page 5 line 17  
- The relationship of cyclin B1 to liver injury and regeneration should be described more or is 
it a typing error on pa ge 5 line 17 (Cyclin D1 or B1?)  
- There is nothing mentioned about IL -6 cytokine and its role in liver diseases  
 
Methods:  
Partial hepatectomy - this part needs revision, it is hard to understand what it means: ...by 
removal of the median and left lateral lob es. 4 days before mice were sacrificed after 3h or 48h.  
 
Results:  
- They are clearly showing that the presence of NKT cells affect liver regeneration in used 
mouse model but the authors should be avoided to use the term 'type I NKT cells' if there is 
no pro of for particular type of NKT cells in the paper - page 8 line 43  
- ELISA results for IL -6 in serum should be supported by detection of gene expression for IL -
6 in injured liver to prove the source of IL -6 in blood  
 
Are the methods appropriate and well descr ibed?  
If not, please specify what is required in your comments to the authors.  
 
No 
 
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
 
Yes 
 
Are the conclusions drawn adequately  supported by the data shown?  
If not, please explain in your comments to the authors.  
 
Yes 
 
Are you able to assess any statistics in the manuscript or would you recommend an 
additional statistical review?  
If an additional statistical review is recommended,  please specify what aspects require further 
assessment in your comments to the editors.  
 
I am able to assess the statistics  
 
Quality of written English  
Please indicate the quality of language in the manuscript:  
 
Acceptable  
 
Declaration of competing intere sts 
Please complete a declaration of competing interests, considering the following questions:  
 
1. Have you in the past five years received reimbursements, fees, funding, or salary from an 
organisation that may in any way gain or lose financially from the pub lication of this 
manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares in an organisation that may in any way gain or lose 
financially from the publication of this manuscript, either now or in the future?  
 
3. Do you hold or are you curren tly applying for any patents relating to the content of the 
manuscript?  
 
4. Have you received reimbursements, fees, funding, or salary from an organization that 
holds or has applied for patents relating to the content of the manuscript?  
 
5. Do you have any other  financial competing interests?  
 
6. Do you have any non -financial competing interests in relation to this paper?  
 
If you can answer no to all of the above, write 'I declare that I have no competing interests' 
below. If your reply is yes to any, please give de tails below.  
 
I declare that I have no competing interests  
 
I agree to the open peer review policy of the journal. I understand that my name will be included 
on my report to the authors and, if the manuscript is accepted for publication, my named report 
including any attachments I upload will be posted on the website along with the authors' 
responses. I agree for my report to be made available under an Open Access Creative Commons  
CC-BY license ( http://creativecommons.org/licenses/by/4.0/ ). I understand th at any comments 
which I do not wish to be included in my named report can be included as confidential comments 
to the editors, which will not be published.  
 
I agree to the open peer review policy of the journal  
assistant