Title: Metabolites mediate the causal associations between gut microbiota and NAFLD#a
Mendelian randomization study
Version: 2 Date: 23 Apr 2024
Reviewer's report:
Reviewed for the journal BMC Gastroenterology
The manuscript  Metabolites mediate the causal associations between gut microbiota and
NAFLD#a Mendelian randomization study, From the team in Tianjin, China
Obtained due ethical approval for the development of the research.
Mendelian randomization was used to search for causal connections between exposures and
outcomes. The assessment was based on the 20-item Strobe-MR (Strengthening the Reporting
of Observational Studies in Epidemiology using Mendelian Randomization)
They sought a causal association between the gut microbiota and NAFLD and then between
NAFLD and 1,400 metabolites.
It used microbiota data from 18,000 individuals, and NAFLD/control data from almost 400,000
people, the vast majority of whom were Europeans, which is an important limiting factor in
extrapolating conclusions to other populations.
He selected 1,400 plasma metabolites, also mostly of European origin.
It presents an adequate methodological description, allowing replication of the study in other
populations.
An inversely proportional correlation was identified between Ruminococcus2( an anaerobic,
Gram-positive Clostridia class bacterium), and NAFLD, so that the presence of this bacterium
in the microbiota reduces the risk of developing NAFLD.
After evaluating the 1,400 metabolites, 7 were identified with a potential relationship with
NAFLD, but after evaluating the Linkage disequilibrium score regression, a relationship
was defined only between 3-indoleglyoxylic acid and .cysteine-glutathione disulfide with
Ruminococcus2.
Despite being methodologically different, previous studies present a conflicting relationship
with the findings between Ruminococcus and NAFLD (doi: 10.1002/hep.28356), which can be
evaluated in the discussion.
Therefore, I believe that the research can add valuable information on the subject, and should
be accepted for publication.
Title: Metabolites mediate the causal associations between gut microbiota and NAFLD#a
Mendelian randomization study
Version: 2 Date: 23 Apr 2024
Reviewer's report:
Reviewed for the journal BMC Gastroenterology
The manuscript  Metabolites mediate the causal associations between gut microbiota and
NAFLD#a Mendelian randomization study, From the team in Tianjin, China
Obtained due ethical approval for the development of the research.
Mendelian randomization was used to search for causal connections between exposures and
outcomes. The assessment was based on the 20-item Strobe-MR (Strengthening the Reporting
of Observational Studies in Epidemiology using Mendelian Randomization)
They sought a causal association between the gut microbiota and NAFLD and then between
NAFLD and 1,400 metabolites.
It used microbiota data from 18,000 individuals, and NAFLD/control data from almost 400,000
people, the vast majority of whom were Europeans, which is an important limiting factor in
extrapolating conclusions to other populations.
He selected 1,400 plasma metabolites, also mostly of European origin.
It presents an adequate methodological description, allowing replication of the study in other
populations.
An inversely proportional correlation was identified between Ruminococcus2( an anaerobic,
Gram-positive Clostridia class bacterium), and NAFLD, so that the presence of this bacterium
in the microbiota reduces the risk of developing NAFLD.
After evaluating the 1,400 metabolites, 7 were identified with a potential relationship with
NAFLD, but after evaluating the Linkage disequilibrium score regression, a relationship
was defined only between 3-indoleglyoxylic acid and .cysteine-glutathione disulfide with
Ruminococcus2.
Despite being methodologically different, previous studies present a conflicting relationship
with the findings between Ruminococcus and NAFLD (doi: 10.1002/hep.28356), which can be
evaluated in the discussion.
Therefore, I believe that the research can add valuable information on the subject, and should
be accepted for publication.
Please review this paper.
user
Title: Metabolites mediate the causal associations between gut microbiota and NAFLD#a
Mendelian randomization study
Version: 2 Date: 23 Apr 2024
Reviewer's report:
Reviewed for the journal BMC Gastroenterology
The manuscript  Metabolites mediate the causal associations between gut microbiota and
NAFLD#a Mendelian randomization study, From the team in Tianjin, China
Obtained due ethical approval for the development of the research.
Mendelian randomization was used to search for causal connections between exposures and
outcomes. The assessment was based on the 20-item Strobe-MR (Strengthening the Reporting
of Observational Studies in Epidemiology using Mendelian Randomization)
They sought a causal association between the gut microbiota and NAFLD and then between
NAFLD and 1,400 metabolites.
It used microbiota data from 18,000 individuals, and NAFLD/control data from almost 400,000
people, the vast majority of whom were Europeans, which is an important limiting factor in
extrapolating conclusions to other populations.
He selected 1,400 plasma metabolites, also mostly of European origin.
It presents an adequate methodological description, allowing replication of the study in other
populations.
An inversely proportional correlation was identified between Ruminococcus2( an anaerobic,
Gram-positive Clostridia class bacterium), and NAFLD, so that the presence of this bacterium
in the microbiota reduces the risk of developing NAFLD.
After evaluating the 1,400 metabolites, 7 were identified with a potential relationship with
NAFLD, but after evaluating the Linkage disequilibrium score regression, a relationship
was defined only between 3-indoleglyoxylic acid and .cysteine-glutathione disulfide with
Ruminococcus2.
Despite being methodologically different, previous studies present a conflicting relationship
with the findings between Ruminococcus and NAFLD (doi: 10.1002/hep.28356), which can be
evaluated in the discussion.
Therefore, I believe that the research can add valuable information on the subject, and should
be accepted for publication.
assistant