Reviewer ’s report  
 
Title: Survivin Expression Pattern in the Intestine of Normoxic and Ischemic Rats  
 
Version: 0 Date: 08 Oct 2016  
 
Reviewer: Maria Grazia Zizzo  
 
Reviewer's report:  
 
The authors  demonstrate that survivin , in normal conditions,is expressed both in the the cells of 
the epithelial lining of the small  intestine and in the colon without any regional difference . 
Survivin is  co -expressed with proliferation marker Ki -67 . After ischemic insult the level of 
survivin   expression is unchanged indicating  that the regenerative capacity of the intestine 
rema ins intact even if   large parts of intestinal  tissue are destroyed  
The paper is generally well written and easy to follow. However, there are a few unclear 
description and statements. Please find them below and revise accordingly.  
How can you explain tha t the tissue can be repaired even if the survivin is not increased ? Can 
you speculate the action mechanism ?  
 I suggest to add in the discussion more informations about the role of survivin in pathological 
condictions  
Are the methods appropriate and well described?  
If not, please specify what is required in your comments to the authors.  
 
Yes 
 
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
 
Yes 
 
Are the conclusions drawn adequately supported by the data shown?  
If not, please explain in your comments to the authors.  
 
Yes 
 
Are you able to assess any statistics in the manuscript or would you recommend an  
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects require further  
assessment in your comments to the editors.  
 
I am able to assess the statistics  
 
Quality o f written English  
Please indicate the quality of language in the manuscript:  
 
Acceptable  
 
Declaration of competing interests  
Please complete a declaration of competing interests, considering the following questions:  
 
1. Have you in the past five years received reimbursements, fees, funding, or salary from an  
organisation that may in any way gain or lose financially from the publication of this  
manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares i n an organisation that may in any way gain or lose  
financially from the publication of this manuscript, either now or in the future?  
 
3. Do you hold or are you currently applying for any patents relating to the content of the  
manuscript?  
 
4. Have you receive d reimbursements, fees, funding, or salary from an organization that 
holds  or has applied for patents relating to the content of the manuscript?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have any non -financial competing interests in  relation to this paper?  
 
If you can answer no to all of the above, write 'I declare that I have no competing interests' 
below.  If your reply is yes to any, please give details below.  
 
'I declare that I have no competing interests'  
 
I agree to the open peer r eview policy of the journal. I understand that my name will be included  
on my report to the authors and, if the manuscript is accepted for publication, my named report  
including any attachments I upload will be posted on the website along with the authors'  
responses. I agree for my report to be made available under an Open Access Creative Commons  
CC-BY license ( http://creativecommons.org/licenses/by/4.0/ ). I understand that any comments  
which I do not wish to be included in my named report can be included a s confidential comments  
to the editors, which will not be published.  
 
I agree to the open peer review policy of the journal  
Reviewer ’s report  
 
Title: Survivin Expression Pattern in the Intestine of Normoxic and Ischemic Rats  
 
Version: 0 Date: 08 Oct 2016  
 
Reviewer: Maria Grazia Zizzo  
 
Reviewer's report:  
 
The authors  demonstrate that survivin , in normal conditions,is expressed both in the the cells of 
the epithelial lining of the small  intestine and in the colon without any regional difference . 
Survivin is  co -expressed with proliferation marker Ki -67 . After ischemic insult the level of 
survivin   expression is unchanged indicating  that the regenerative capacity of the intestine 
rema ins intact even if   large parts of intestinal  tissue are destroyed  
The paper is generally well written and easy to follow. However, there are a few unclear 
description and statements. Please find them below and revise accordingly.  
How can you explain tha t the tissue can be repaired even if the survivin is not increased ? Can 
you speculate the action mechanism ?  
 I suggest to add in the discussion more informations about the role of survivin in pathological 
condictions  
Are the methods appropriate and well described?  
If not, please specify what is required in your comments to the authors.  
 
Yes 
 
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
 
Yes 
 
Are the conclusions drawn adequately supported by the data shown?  
If not, please explain in your comments to the authors.  
 
Yes 
 
Are you able to assess any statistics in the manuscript or would you recommend an  
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects require further  
assessment in your comments to the editors.  
 
I am able to assess the statistics  
 
Quality o f written English  
Please indicate the quality of language in the manuscript:  
 
Acceptable  
 
Declaration of competing interests  
Please complete a declaration of competing interests, considering the following questions:  
 
1. Have you in the past five years received reimbursements, fees, funding, or salary from an  
organisation that may in any way gain or lose financially from the publication of this  
manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares i n an organisation that may in any way gain or lose  
financially from the publication of this manuscript, either now or in the future?  
 
3. Do you hold or are you currently applying for any patents relating to the content of the  
manuscript?  
 
4. Have you receive d reimbursements, fees, funding, or salary from an organization that 
holds  or has applied for patents relating to the content of the manuscript?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have any non -financial competing interests in  relation to this paper?  
 
If you can answer no to all of the above, write 'I declare that I have no competing interests' 
below.  If your reply is yes to any, please give details below.  
 
'I declare that I have no competing interests'  
 
I agree to the open peer r eview policy of the journal. I understand that my name will be included  
on my report to the authors and, if the manuscript is accepted for publication, my named report  
including any attachments I upload will be posted on the website along with the authors'  
responses. I agree for my report to be made available under an Open Access Creative Commons  
CC-BY license ( http://creativecommons.org/licenses/by/4.0/ ). I understand that any comments  
which I do not wish to be included in my named report can be included a s confidential comments  
to the editors, which will not be published.  
 
I agree to the open peer review policy of the journal  
Please review this paper.
user
Reviewer ’s report  
 
Title: Survivin Expression Pattern in the Intestine of Normoxic and Ischemic Rats  
 
Version: 0 Date: 08 Oct 2016  
 
Reviewer: Maria Grazia Zizzo  
 
Reviewer's report:  
 
The authors  demonstrate that survivin , in normal conditions,is expressed both in the the cells of 
the epithelial lining of the small  intestine and in the colon without any regional difference . 
Survivin is  co -expressed with proliferation marker Ki -67 . After ischemic insult the level of 
survivin   expression is unchanged indicating  that the regenerative capacity of the intestine 
rema ins intact even if   large parts of intestinal  tissue are destroyed  
The paper is generally well written and easy to follow. However, there are a few unclear 
description and statements. Please find them below and revise accordingly.  
How can you explain tha t the tissue can be repaired even if the survivin is not increased ? Can 
you speculate the action mechanism ?  
 I suggest to add in the discussion more informations about the role of survivin in pathological 
condictions  
Are the methods appropriate and well described?  
If not, please specify what is required in your comments to the authors.  
 
Yes 
 
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
 
Yes 
 
Are the conclusions drawn adequately supported by the data shown?  
If not, please explain in your comments to the authors.  
 
Yes 
 
Are you able to assess any statistics in the manuscript or would you recommend an  
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects require further  
assessment in your comments to the editors.  
 
I am able to assess the statistics  
 
Quality o f written English  
Please indicate the quality of language in the manuscript:  
 
Acceptable  
 
Declaration of competing interests  
Please complete a declaration of competing interests, considering the following questions:  
 
1. Have you in the past five years received reimbursements, fees, funding, or salary from an  
organisation that may in any way gain or lose financially from the publication of this  
manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares i n an organisation that may in any way gain or lose  
financially from the publication of this manuscript, either now or in the future?  
 
3. Do you hold or are you currently applying for any patents relating to the content of the  
manuscript?  
 
4. Have you receive d reimbursements, fees, funding, or salary from an organization that 
holds  or has applied for patents relating to the content of the manuscript?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have any non -financial competing interests in  relation to this paper?  
 
If you can answer no to all of the above, write 'I declare that I have no competing interests' 
below.  If your reply is yes to any, please give details below.  
 
'I declare that I have no competing interests'  
 
I agree to the open peer r eview policy of the journal. I understand that my name will be included  
on my report to the authors and, if the manuscript is accepted for publication, my named report  
including any attachments I upload will be posted on the website along with the authors'  
responses. I agree for my report to be made available under an Open Access Creative Commons  
CC-BY license ( http://creativecommons.org/licenses/by/4.0/ ). I understand that any comments  
which I do not wish to be included in my named report can be included a s confidential comments  
to the editors, which will not be published.  
 
I agree to the open peer review policy of the journal  
assistant