Title: Unravelling the role of beta-CGRP in inflammatory bowel disease and its potential role
in gastrointestinal homeostasis
Version: 3 Date: 20 Jun 2024
Reviewer's report:
This study observed the circulating levels of beta calcitonin gene-related peptide (beta-CGRP)
measured by ELISA in patients with IBD in comparison with healthy controls (HC) and chronic
migraine (CM) patients. The results showed that the circulating levels of beta-CGRP in both
ulcerative colitis (UC) and Crohn's disease (CD) patients were significantly lower than those
in HC and CM. However, this is only an observed phenomenon in IBD patients in this clinical
study which did nothing to deal with the pathophysiological and protective role of beta-CGRP so
that the conclusion regarding the pathophysiological and protective role of this neuropeptide is
somehow groundless. One needs to perform intervention study, for example, to use beta-CGRP
or its agonist during the induction of experimental IBD in order to study its pathophysiological
and protective role. Therefore, it is advisable to reach the conclusion of this paper in a logical
way from its results. Of course, the authors can discuss the pathophysiological and protective
role of beta-CGRP by citing literatures in their discussions.  By the way, apart from ELISA,
another method needs to be used to measure the circulating levels of this neuropeptide for its
reproducibility.
Title: Unravelling the role of beta-CGRP in inflammatory bowel disease and its potential role
in gastrointestinal homeostasis
Version: 3 Date: 20 Jun 2024
Reviewer's report:
This study observed the circulating levels of beta calcitonin gene-related peptide (beta-CGRP)
measured by ELISA in patients with IBD in comparison with healthy controls (HC) and chronic
migraine (CM) patients. The results showed that the circulating levels of beta-CGRP in both
ulcerative colitis (UC) and Crohn's disease (CD) patients were significantly lower than those
in HC and CM. However, this is only an observed phenomenon in IBD patients in this clinical
study which did nothing to deal with the pathophysiological and protective role of beta-CGRP so
that the conclusion regarding the pathophysiological and protective role of this neuropeptide is
somehow groundless. One needs to perform intervention study, for example, to use beta-CGRP
or its agonist during the induction of experimental IBD in order to study its pathophysiological
and protective role. Therefore, it is advisable to reach the conclusion of this paper in a logical
way from its results. Of course, the authors can discuss the pathophysiological and protective
role of beta-CGRP by citing literatures in their discussions.  By the way, apart from ELISA,
another method needs to be used to measure the circulating levels of this neuropeptide for its
reproducibility.
Please review this paper.
user
Title: Unravelling the role of beta-CGRP in inflammatory bowel disease and its potential role
in gastrointestinal homeostasis
Version: 3 Date: 20 Jun 2024
Reviewer's report:
This study observed the circulating levels of beta calcitonin gene-related peptide (beta-CGRP)
measured by ELISA in patients with IBD in comparison with healthy controls (HC) and chronic
migraine (CM) patients. The results showed that the circulating levels of beta-CGRP in both
ulcerative colitis (UC) and Crohn's disease (CD) patients were significantly lower than those
in HC and CM. However, this is only an observed phenomenon in IBD patients in this clinical
study which did nothing to deal with the pathophysiological and protective role of beta-CGRP so
that the conclusion regarding the pathophysiological and protective role of this neuropeptide is
somehow groundless. One needs to perform intervention study, for example, to use beta-CGRP
or its agonist during the induction of experimental IBD in order to study its pathophysiological
and protective role. Therefore, it is advisable to reach the conclusion of this paper in a logical
way from its results. Of course, the authors can discuss the pathophysiological and protective
role of beta-CGRP by citing literatures in their discussions.  By the way, apart from ELISA,
another method needs to be used to measure the circulating levels of this neuropeptide for its
reproducibility.
assistant