Title: A novel role for Helicobacter pylori cytotoxin-associated gene A in negative regulation
of autophagy in human gastric cells.
Version: 3 Date: 17 Jun 2023
Reviewer's report:
I think the authors revised their manuscript well according to my suggestions. I think this
manuscript is suitable to be published in BMC Gastroenterology after dealing with the following
point:
I asked authors to provide a description about P62 data, and authors explained the reason
why they didn't as below. I agree with the authors' point and I suggest authors to provide
the explanation not only to me but also to future readers by including the explanation in the
manuscript.
------------------
Comment 8: Fig 1B: P62 also is an important marker of autophagy. It would be better if a
description about P62 data is provided.
Response: As you mentioned, P62 is a marker of autophagy. However, we were concerned
that we did not measure it in subsequent experiments and could not provide P62 data in
the manuscript. We did not measure it for several reasons. First, we believe that the most
valuable evaluation of autophagic flux is the assessment of the formation and degradation
of autophagosomes, and we assessed autophagic flux using interpretation data from LC3-II
immunoblotting. Second, the timing of the increase or decrease in P62 does not necessarily
coincide with that of LC3. Third, factors other than autophagy may be involved in the production
and decrease of P62.
------------------
Title: A novel role for Helicobacter pylori cytotoxin-associated gene A in negative regulation
of autophagy in human gastric cells.
Version: 3 Date: 17 Jun 2023
Reviewer's report:
I think the authors revised their manuscript well according to my suggestions. I think this
manuscript is suitable to be published in BMC Gastroenterology after dealing with the following
point:
I asked authors to provide a description about P62 data, and authors explained the reason
why they didn't as below. I agree with the authors' point and I suggest authors to provide
the explanation not only to me but also to future readers by including the explanation in the
manuscript.
------------------
Comment 8: Fig 1B: P62 also is an important marker of autophagy. It would be better if a
description about P62 data is provided.
Response: As you mentioned, P62 is a marker of autophagy. However, we were concerned
that we did not measure it in subsequent experiments and could not provide P62 data in
the manuscript. We did not measure it for several reasons. First, we believe that the most
valuable evaluation of autophagic flux is the assessment of the formation and degradation
of autophagosomes, and we assessed autophagic flux using interpretation data from LC3-II
immunoblotting. Second, the timing of the increase or decrease in P62 does not necessarily
coincide with that of LC3. Third, factors other than autophagy may be involved in the production
and decrease of P62.
------------------
Please review this paper.
user
Title: A novel role for Helicobacter pylori cytotoxin-associated gene A in negative regulation
of autophagy in human gastric cells.
Version: 3 Date: 17 Jun 2023
Reviewer's report:
I think the authors revised their manuscript well according to my suggestions. I think this
manuscript is suitable to be published in BMC Gastroenterology after dealing with the following
point:
I asked authors to provide a description about P62 data, and authors explained the reason
why they didn't as below. I agree with the authors' point and I suggest authors to provide
the explanation not only to me but also to future readers by including the explanation in the
manuscript.
------------------
Comment 8: Fig 1B: P62 also is an important marker of autophagy. It would be better if a
description about P62 data is provided.
Response: As you mentioned, P62 is a marker of autophagy. However, we were concerned
that we did not measure it in subsequent experiments and could not provide P62 data in
the manuscript. We did not measure it for several reasons. First, we believe that the most
valuable evaluation of autophagic flux is the assessment of the formation and degradation
of autophagosomes, and we assessed autophagic flux using interpretation data from LC3-II
immunoblotting. Second, the timing of the increase or decrease in P62 does not necessarily
coincide with that of LC3. Third, factors other than autophagy may be involved in the production
and decrease of P62.
------------------
assistant