Reviewer's report
Title:The Pancreatic Zymogen Granule Membrane Protein, GP2, Binds
Escherichia coli Type 1 Fimbriae
Version: 3Date:8 June 2009
Reviewer: Luis Angel Fernández Herrero
Reviewer's report:
The revised manuscript has been improved to a reasonable extent, especially
because the wild speculations regarding the anti-infective role of GP2 in the gut,
which are now only mentioned in the Discussion. Binding of recombinant GP2 to
bacteria expressing type I fimbriae and FimH is established, although its
biological significant remains unsolved. A comparison of the binding to GP2 and
Uromodulin is now shown. A direct binding assay between purified FimH and
GP2 is still missing, but it may not be essential for this manuscript. Data are well
presented and the paper is brief and easy to read. Minor text corrections have
been made. The manuscript may be of sufficient quality to merit publication.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
'I declare that I have no competing interests'
Reviewer's report
Title:The Pancreatic Zymogen Granule Membrane Protein, GP2, Binds
Escherichia coli Type 1 Fimbriae
Version: 3Date:8 June 2009
Reviewer: Luis Angel Fernández Herrero
Reviewer's report:
The revised manuscript has been improved to a reasonable extent, especially
because the wild speculations regarding the anti-infective role of GP2 in the gut,
which are now only mentioned in the Discussion. Binding of recombinant GP2 to
bacteria expressing type I fimbriae and FimH is established, although its
biological significant remains unsolved. A comparison of the binding to GP2 and
Uromodulin is now shown. A direct binding assay between purified FimH and
GP2 is still missing, but it may not be essential for this manuscript. Data are well
presented and the paper is brief and easy to read. Minor text corrections have
been made. The manuscript may be of sufficient quality to merit publication.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
'I declare that I have no competing interests'
Please review this paper.
user
Reviewer's report
Title:The Pancreatic Zymogen Granule Membrane Protein, GP2, Binds
Escherichia coli Type 1 Fimbriae
Version: 3Date:8 June 2009
Reviewer: Luis Angel Fernández Herrero
Reviewer's report:
The revised manuscript has been improved to a reasonable extent, especially
because the wild speculations regarding the anti-infective role of GP2 in the gut,
which are now only mentioned in the Discussion. Binding of recombinant GP2 to
bacteria expressing type I fimbriae and FimH is established, although its
biological significant remains unsolved. A comparison of the binding to GP2 and
Uromodulin is now shown. A direct binding assay between purified FimH and
GP2 is still missing, but it may not be essential for this manuscript. Data are well
presented and the paper is brief and easy to read. Minor text corrections have
been made. The manuscript may be of sufficient quality to merit publication.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
'I declare that I have no competing interests'
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