Reviewer's report
Title:Intestinal permeability - a new target for disease prevention and therapy?
Version:3Date:1 May 2014
Reviewer: Robin Spiller
Reviewer's report:
This is a wide ranging review of a subject of great current interest which reflects
the expertise of the international group who produced it. Like many multi-author
manuscripts it need a bit of editing to remove repetition and shorten it a bit.
I very much enjoyed reading it and I think it will be useful to be field.
Comments
1.The first 6 pages defining the terms should be shortened as it does repeat
itself.
2. The section on cannabinoids is very brief. It really does not justify a separate
section unless it is expanded with more references.
3. The section on microbiota has an introduction which can be omitted and
simply referenced. It overlaps so much with many other recent reviews.
4. Page 11 paragraph 2 More comment is warranted on the utilisation of mucus
as an energy source and the role of commensal bacteria in mucus degradation.
There is much current interest in the role of mucus derived sialic acid in pathogen
expansion after antibiotic use 1.
5. Page 12 Here it would be important to mention how Na+-dependent transport
of nutrients such as glucose and alanine increase permeability of tight junctions
2.
6. Page 15 The comment that TNFa increases permeability needs a caveat since
in some models it actually increases the barrier 3.
7. The section on measurement seems out of place. As a methodological section
should it not go at the beginning?
8. Page 17 Should you not mention the effect of glutamine which has been
shown to correct impaired barrier function.4.
9. Not sure I agree that butyrate deficiency is an indirect marker of permeability.
The reference quoted does not validate this in humans.
6.Minor typos
References have dual numbering e.g. 91 (78) please correct
Reference List
(1) Ng KM, Ferreyra JA, Higginbottom SK, Lynch JB, Kashyap PC, Gopinath S et
al. Microbiota-liberated host sugars facilitate post-antibiotic expansion of enteric
pathogens. Nature 2013; 502(7469):96-99.
(2) Turner JR. Show me the pathway! Regulation of paracellular permeability by
Na(+)-glucose cotransport. Adv Drug Deliv Rev 2000; 41(3):265-281.
(3) Corridoni D, Pastorelli L, Mattioli B, Locovei S, Ishikawa D, Arseneau KO et
al. Probiotic bacteria regulate intestinal epithelial permeability in experimental
ileitis by a TNF-dependent mechanism. PLoS One 2012; 7(7):e42067.
(4) Yoshida S, Matsui M, Shirouzu Y, Fujita H, Yamana H, Shirouzu K. Effects of
glutamine supplements and radiochemotherapy on systemic immune and gut
barrier function in patients with advanced esophageal cancer. Ann Surg 1998;
227(4):485-491.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Intestinal permeability - a new target for disease prevention and therapy?
Version:3Date:1 May 2014
Reviewer: Robin Spiller
Reviewer's report:
This is a wide ranging review of a subject of great current interest which reflects
the expertise of the international group who produced it. Like many multi-author
manuscripts it need a bit of editing to remove repetition and shorten it a bit.
I very much enjoyed reading it and I think it will be useful to be field.
Comments
1.The first 6 pages defining the terms should be shortened as it does repeat
itself.
2. The section on cannabinoids is very brief. It really does not justify a separate
section unless it is expanded with more references.
3. The section on microbiota has an introduction which can be omitted and
simply referenced. It overlaps so much with many other recent reviews.
4. Page 11 paragraph 2 More comment is warranted on the utilisation of mucus
as an energy source and the role of commensal bacteria in mucus degradation.
There is much current interest in the role of mucus derived sialic acid in pathogen
expansion after antibiotic use 1.
5. Page 12 Here it would be important to mention how Na+-dependent transport
of nutrients such as glucose and alanine increase permeability of tight junctions
2.
6. Page 15 The comment that TNFa increases permeability needs a caveat since
in some models it actually increases the barrier 3.
7. The section on measurement seems out of place. As a methodological section
should it not go at the beginning?
8. Page 17 Should you not mention the effect of glutamine which has been
shown to correct impaired barrier function.4.
9. Not sure I agree that butyrate deficiency is an indirect marker of permeability.
The reference quoted does not validate this in humans.
6.Minor typos
References have dual numbering e.g. 91 (78) please correct
Reference List
(1) Ng KM, Ferreyra JA, Higginbottom SK, Lynch JB, Kashyap PC, Gopinath S et
al. Microbiota-liberated host sugars facilitate post-antibiotic expansion of enteric
pathogens. Nature 2013; 502(7469):96-99.
(2) Turner JR. Show me the pathway! Regulation of paracellular permeability by
Na(+)-glucose cotransport. Adv Drug Deliv Rev 2000; 41(3):265-281.
(3) Corridoni D, Pastorelli L, Mattioli B, Locovei S, Ishikawa D, Arseneau KO et
al. Probiotic bacteria regulate intestinal epithelial permeability in experimental
ileitis by a TNF-dependent mechanism. PLoS One 2012; 7(7):e42067.
(4) Yoshida S, Matsui M, Shirouzu Y, Fujita H, Yamana H, Shirouzu K. Effects of
glutamine supplements and radiochemotherapy on systemic immune and gut
barrier function in patients with advanced esophageal cancer. Ann Surg 1998;
227(4):485-491.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Intestinal permeability - a new target for disease prevention and therapy?
Version:3Date:1 May 2014
Reviewer: Robin Spiller
Reviewer's report:
This is a wide ranging review of a subject of great current interest which reflects
the expertise of the international group who produced it. Like many multi-author
manuscripts it need a bit of editing to remove repetition and shorten it a bit.
I very much enjoyed reading it and I think it will be useful to be field.
Comments
1.The first 6 pages defining the terms should be shortened as it does repeat
itself.
2. The section on cannabinoids is very brief. It really does not justify a separate
section unless it is expanded with more references.
3. The section on microbiota has an introduction which can be omitted and
simply referenced. It overlaps so much with many other recent reviews.
4. Page 11 paragraph 2 More comment is warranted on the utilisation of mucus
as an energy source and the role of commensal bacteria in mucus degradation.
There is much current interest in the role of mucus derived sialic acid in pathogen
expansion after antibiotic use 1.
5. Page 12 Here it would be important to mention how Na+-dependent transport
of nutrients such as glucose and alanine increase permeability of tight junctions
2.
6. Page 15 The comment that TNFa increases permeability needs a caveat since
in some models it actually increases the barrier 3.
7. The section on measurement seems out of place. As a methodological section
should it not go at the beginning?
8. Page 17 Should you not mention the effect of glutamine which has been
shown to correct impaired barrier function.4.
9. Not sure I agree that butyrate deficiency is an indirect marker of permeability.
The reference quoted does not validate this in humans.
6.Minor typos
References have dual numbering e.g. 91 (78) please correct
Reference List
(1) Ng KM, Ferreyra JA, Higginbottom SK, Lynch JB, Kashyap PC, Gopinath S et
al. Microbiota-liberated host sugars facilitate post-antibiotic expansion of enteric
pathogens. Nature 2013; 502(7469):96-99.
(2) Turner JR. Show me the pathway! Regulation of paracellular permeability by
Na(+)-glucose cotransport. Adv Drug Deliv Rev 2000; 41(3):265-281.
(3) Corridoni D, Pastorelli L, Mattioli B, Locovei S, Ishikawa D, Arseneau KO et
al. Probiotic bacteria regulate intestinal epithelial permeability in experimental
ileitis by a TNF-dependent mechanism. PLoS One 2012; 7(7):e42067.
(4) Yoshida S, Matsui M, Shirouzu Y, Fujita H, Yamana H, Shirouzu K. Effects of
glutamine supplements and radiochemotherapy on systemic immune and gut
barrier function in patients with advanced esophageal cancer. Ann Surg 1998;
227(4):485-491.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I declare that I have no competing interests
assistant