Reviewer's report 
 Transfection of IL-10 expression vectors into endothelial cultures attenuates alpha 4 beta Title: 
7-dependent lymphocyte adhesion mediated by MAdCAM-1 
Authors: 
Makoto Sasaki ( msasak@lsuhsc.edu )
Paul Jordan ( pjorda1@lsuhsc.edu )
Jeff Houghton ( jhough@lsuhsc.edu )
Xianmin Meng ( mengderm@yahoo.com )
Makoto Itoh ( mitoh@med.nagoya-cu.ac.jp )
Takashi Joh ( tjoh@med.nagoya-cu.ac.jp )
J. S. Alexander ( jalexa@lsuhsc.edu )
 1  14 Jan 2003 Version: Date: 
 David Binion Reviewer: 
 A paper of considerable general medical or scientific interest Level of interest: 
 Accept without revision Advice on publication: 
The paper is a straightforward and interesting report of the inhibition of MAdCAM-1 expression in a 
mouse endothelial cell line using IL-10 transfection. This is the first report of MAdCAM-1 inhibition using 
a transfection based strategy. The data is succinct and convincing, and is an important contribution to 
the area of experimental microvascular gene therapy, with potential relevance to human IBD. 
Competing interests: 
None declared. 
Reviewer's report 
 Transfection of IL-10 expression vectors into endothelial cultures attenuates alpha 4 beta Title: 
7-dependent lymphocyte adhesion mediated by MAdCAM-1 
Authors: 
Makoto Sasaki ( msasak@lsuhsc.edu )
Paul Jordan ( pjorda1@lsuhsc.edu )
Jeff Houghton ( jhough@lsuhsc.edu )
Xianmin Meng ( mengderm@yahoo.com )
Makoto Itoh ( mitoh@med.nagoya-cu.ac.jp )
Takashi Joh ( tjoh@med.nagoya-cu.ac.jp )
J. S. Alexander ( jalexa@lsuhsc.edu )
 1  14 Jan 2003 Version: Date: 
 David Binion Reviewer: 
 A paper of considerable general medical or scientific interest Level of interest: 
 Accept without revision Advice on publication: 
The paper is a straightforward and interesting report of the inhibition of MAdCAM-1 expression in a 
mouse endothelial cell line using IL-10 transfection. This is the first report of MAdCAM-1 inhibition using 
a transfection based strategy. The data is succinct and convincing, and is an important contribution to 
the area of experimental microvascular gene therapy, with potential relevance to human IBD. 
Competing interests: 
None declared. 
Please review this paper.
user
Reviewer's report 
 Transfection of IL-10 expression vectors into endothelial cultures attenuates alpha 4 beta Title: 
7-dependent lymphocyte adhesion mediated by MAdCAM-1 
Authors: 
Makoto Sasaki ( msasak@lsuhsc.edu )
Paul Jordan ( pjorda1@lsuhsc.edu )
Jeff Houghton ( jhough@lsuhsc.edu )
Xianmin Meng ( mengderm@yahoo.com )
Makoto Itoh ( mitoh@med.nagoya-cu.ac.jp )
Takashi Joh ( tjoh@med.nagoya-cu.ac.jp )
J. S. Alexander ( jalexa@lsuhsc.edu )
 1  14 Jan 2003 Version: Date: 
 David Binion Reviewer: 
 A paper of considerable general medical or scientific interest Level of interest: 
 Accept without revision Advice on publication: 
The paper is a straightforward and interesting report of the inhibition of MAdCAM-1 expression in a 
mouse endothelial cell line using IL-10 transfection. This is the first report of MAdCAM-1 inhibition using 
a transfection based strategy. The data is succinct and convincing, and is an important contribution to 
the area of experimental microvascular gene therapy, with potential relevance to human IBD. 
Competing interests: 
None declared. 
assistant