Reviewer's report
Title:Analysis of cell cycle-related proteins in gastric intramucosal
differentiated-type cancers based on mucin phenotypes: a new concept of
molecular carcinogenesis for early differentiated-type gastric cancers
Version: 1Date:30 November 2009
Reviewer: Shuho Semba
Reviewer's report:
The analysis of cell cycle-related proteins in gastric intramucosal
differentiated-type cancers based on mucin phenotypes: a new concept of
molecular carcinogenesis for early differentiated-type gastric cancers (Sugai et
al.) In this study, the authors investigated expression patterns of cell
cycle-related proteins in the early phase of differentiated-type gastric cancers
removed by ESD. The concept of this study seems good and the results are well
arranged. However, the reviewer recommends to re-prepare the manuscript
along with the points below.
1. The authors subclassified the gastric cancers analyzed according to the
expression patterns of the mucins. However, the Cdx2 status should be included
into the decision of these mucin phenotypes because Cdx2 plays an important
role for intestinal differentiation of gastric epithelia. For example, tumors showing
Cdx2+/CD10+/MUC2+ will be regarded as the intestinal-type gastric cancers and
tumors showing Cdx2-/MUC5AC+/MUC6+ will be included into the gastric-type
gastric cancers.
2. The figures need major modification. Prepare these figures in higher resolution
(figure 1 and 3) with the scale bars. And, in figure 2, the results are difficult to
understand. The results of up- and down-regulation of p27 protein are
overlapped? Figure 4 should be re-arranged for better understanding.
3. The descriptions in the results section are long and pointless. The percentages
of the immunohistochemistry are shown in figure 2.
4. The authors did not mention about the genetic background of these early
gastric cancers. Hasuo et al. examined the status of microsatellite instability to
assess the risk of recurrence of gastric cancer after initial ESD (Br J Cancer 96:
89-94, 2007). The authors need to discuss about this point.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:Analysis of cell cycle-related proteins in gastric intramucosal
differentiated-type cancers based on mucin phenotypes: a new concept of
molecular carcinogenesis for early differentiated-type gastric cancers
Version: 1Date:30 November 2009
Reviewer: Shuho Semba
Reviewer's report:
The analysis of cell cycle-related proteins in gastric intramucosal
differentiated-type cancers based on mucin phenotypes: a new concept of
molecular carcinogenesis for early differentiated-type gastric cancers (Sugai et
al.) In this study, the authors investigated expression patterns of cell
cycle-related proteins in the early phase of differentiated-type gastric cancers
removed by ESD. The concept of this study seems good and the results are well
arranged. However, the reviewer recommends to re-prepare the manuscript
along with the points below.
1. The authors subclassified the gastric cancers analyzed according to the
expression patterns of the mucins. However, the Cdx2 status should be included
into the decision of these mucin phenotypes because Cdx2 plays an important
role for intestinal differentiation of gastric epithelia. For example, tumors showing
Cdx2+/CD10+/MUC2+ will be regarded as the intestinal-type gastric cancers and
tumors showing Cdx2-/MUC5AC+/MUC6+ will be included into the gastric-type
gastric cancers.
2. The figures need major modification. Prepare these figures in higher resolution
(figure 1 and 3) with the scale bars. And, in figure 2, the results are difficult to
understand. The results of up- and down-regulation of p27 protein are
overlapped? Figure 4 should be re-arranged for better understanding.
3. The descriptions in the results section are long and pointless. The percentages
of the immunohistochemistry are shown in figure 2.
4. The authors did not mention about the genetic background of these early
gastric cancers. Hasuo et al. examined the status of microsatellite instability to
assess the risk of recurrence of gastric cancer after initial ESD (Br J Cancer 96:
89-94, 2007). The authors need to discuss about this point.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:Analysis of cell cycle-related proteins in gastric intramucosal
differentiated-type cancers based on mucin phenotypes: a new concept of
molecular carcinogenesis for early differentiated-type gastric cancers
Version: 1Date:30 November 2009
Reviewer: Shuho Semba
Reviewer's report:
The analysis of cell cycle-related proteins in gastric intramucosal
differentiated-type cancers based on mucin phenotypes: a new concept of
molecular carcinogenesis for early differentiated-type gastric cancers (Sugai et
al.) In this study, the authors investigated expression patterns of cell
cycle-related proteins in the early phase of differentiated-type gastric cancers
removed by ESD. The concept of this study seems good and the results are well
arranged. However, the reviewer recommends to re-prepare the manuscript
along with the points below.
1. The authors subclassified the gastric cancers analyzed according to the
expression patterns of the mucins. However, the Cdx2 status should be included
into the decision of these mucin phenotypes because Cdx2 plays an important
role for intestinal differentiation of gastric epithelia. For example, tumors showing
Cdx2+/CD10+/MUC2+ will be regarded as the intestinal-type gastric cancers and
tumors showing Cdx2-/MUC5AC+/MUC6+ will be included into the gastric-type
gastric cancers.
2. The figures need major modification. Prepare these figures in higher resolution
(figure 1 and 3) with the scale bars. And, in figure 2, the results are difficult to
understand. The results of up- and down-regulation of p27 protein are
overlapped? Figure 4 should be re-arranged for better understanding.
3. The descriptions in the results section are long and pointless. The percentages
of the immunohistochemistry are shown in figure 2.
4. The authors did not mention about the genetic background of these early
gastric cancers. Hasuo et al. examined the status of microsatellite instability to
assess the risk of recurrence of gastric cancer after initial ESD (Br J Cancer 96:
89-94, 2007). The authors need to discuss about this point.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
I declare that I have no competing interests.
assistant