Reviewer's report
Title:IGF-1 attenuates portal hypertension by decreasing portal vein endotoxin
via regulating intestinal tight junctions in cirrhotic rats
Version:4Date:23 March 2015
Reviewer: Jesus S Prieto
Reviewer's report:
The paper addresses an important issue with great clinical relevance, that is the
fact that IGF-I restores the deranged intestinal barrier present in cirrhotic rats and
that this hormone may have a relevant role in the treatment of liver cirrhosis
Major compulsory revisions:
1) Association of two findings do not indicate causal relationship among them.
Thus, although portal hypertension in cirrhotic rats is associated with reduced
occludin and claudin expression and IGF1 restores intestinal barrier function
accompanied by occludin and claudin upregulation, this mere coindence of
effects is no proof of a causal relationship. Therefore the text of the manuscript
should mention that improvement of tight junctions might be one possible
mechamism, together with reduced enterocyte apoptosis, explaining the
restoration of intestinal barrier function mediated by IGF-1.
2) PCR data should be presented by means of real-time quantitative PCR using
the appropriate instruments (and not by densitometry of the PCR bands). If the
authors do not have access to this methodology they should present only WB
data
3) The authors should revise the bibliography. In page 9 (fourth line from the
bottom) they state that IGF-1 displays trophic effects on intestinal endothelium. Is
this correct or do they refer to the intestinal epithelium?
4) page 10 (second paragraph) : IGF-1 instead of HGF-1
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
None
Reviewer's report
Title:IGF-1 attenuates portal hypertension by decreasing portal vein endotoxin
via regulating intestinal tight junctions in cirrhotic rats
Version:4Date:23 March 2015
Reviewer: Jesus S Prieto
Reviewer's report:
The paper addresses an important issue with great clinical relevance, that is the
fact that IGF-I restores the deranged intestinal barrier present in cirrhotic rats and
that this hormone may have a relevant role in the treatment of liver cirrhosis
Major compulsory revisions:
1) Association of two findings do not indicate causal relationship among them.
Thus, although portal hypertension in cirrhotic rats is associated with reduced
occludin and claudin expression and IGF1 restores intestinal barrier function
accompanied by occludin and claudin upregulation, this mere coindence of
effects is no proof of a causal relationship. Therefore the text of the manuscript
should mention that improvement of tight junctions might be one possible
mechamism, together with reduced enterocyte apoptosis, explaining the
restoration of intestinal barrier function mediated by IGF-1.
2) PCR data should be presented by means of real-time quantitative PCR using
the appropriate instruments (and not by densitometry of the PCR bands). If the
authors do not have access to this methodology they should present only WB
data
3) The authors should revise the bibliography. In page 9 (fourth line from the
bottom) they state that IGF-1 displays trophic effects on intestinal endothelium. Is
this correct or do they refer to the intestinal epithelium?
4) page 10 (second paragraph) : IGF-1 instead of HGF-1
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
None
Please review this paper.
user
Reviewer's report
Title:IGF-1 attenuates portal hypertension by decreasing portal vein endotoxin
via regulating intestinal tight junctions in cirrhotic rats
Version:4Date:23 March 2015
Reviewer: Jesus S Prieto
Reviewer's report:
The paper addresses an important issue with great clinical relevance, that is the
fact that IGF-I restores the deranged intestinal barrier present in cirrhotic rats and
that this hormone may have a relevant role in the treatment of liver cirrhosis
Major compulsory revisions:
1) Association of two findings do not indicate causal relationship among them.
Thus, although portal hypertension in cirrhotic rats is associated with reduced
occludin and claudin expression and IGF1 restores intestinal barrier function
accompanied by occludin and claudin upregulation, this mere coindence of
effects is no proof of a causal relationship. Therefore the text of the manuscript
should mention that improvement of tight junctions might be one possible
mechamism, together with reduced enterocyte apoptosis, explaining the
restoration of intestinal barrier function mediated by IGF-1.
2) PCR data should be presented by means of real-time quantitative PCR using
the appropriate instruments (and not by densitometry of the PCR bands). If the
authors do not have access to this methodology they should present only WB
data
3) The authors should revise the bibliography. In page 9 (fourth line from the
bottom) they state that IGF-1 displays trophic effects on intestinal endothelium. Is
this correct or do they refer to the intestinal epithelium?
4) page 10 (second paragraph) : IGF-1 instead of HGF-1
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
None
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