Reviewer's report
Title:Mucosal Progranulin expression is induced by H. pylori, but independent of
Secretory Leukocyte Protease Inhibitor (SLPI) expression
Version: 1Date:24 April 2011
Reviewer: Antonia Sepulveda
Reviewer's report:
Progranulins are converted to granulins and are secreted by epithelial cells and
immune cells. Progranulin and granulins have been shown to regulate
inflammation, proliferation and wound healing. The authors characterized the
expression of progranulin and granulins in gastric mucosa (by measuring
progranulin) in relation to H. pylori status.
Further, since progranulin was previously shown to be regulated by SLP1 in
SLP1 deficient mice (SLP1 prevents progranulin degradation by elastase) the
authors hypothesized that progranulin levels could be correlated with SLP1 levels
in gastric mucosa and used the AGS cell culture model to determine the effects
of changes in SLP1 levels using SLP1-siRNA.
The study reports that progranulin is up-regulated during H. pylori infection,
however, SLP1 did not appear to have a regulatory role on progranulin in H.
pylori gastritis.
The methods used are appropriate and well described and the conclusions are
supported by the data presented in the paper.
The manuscript is well written, with only rare typos throughout the text, which
should be corrected.
The hypothesis regarding the possible role of SLP1 in progranulin regulation
could be better outlined by stating the hypothesis directly in the introduction and
abstract.
This is a descriptive study, that provides additive information regarding the host
response to H. pylori, suggesting a role for progranulins during H. pylori gastritis.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
'I declare that I have no competing interests'
Reviewer's report
Title:Mucosal Progranulin expression is induced by H. pylori, but independent of
Secretory Leukocyte Protease Inhibitor (SLPI) expression
Version: 1Date:24 April 2011
Reviewer: Antonia Sepulveda
Reviewer's report:
Progranulins are converted to granulins and are secreted by epithelial cells and
immune cells. Progranulin and granulins have been shown to regulate
inflammation, proliferation and wound healing. The authors characterized the
expression of progranulin and granulins in gastric mucosa (by measuring
progranulin) in relation to H. pylori status.
Further, since progranulin was previously shown to be regulated by SLP1 in
SLP1 deficient mice (SLP1 prevents progranulin degradation by elastase) the
authors hypothesized that progranulin levels could be correlated with SLP1 levels
in gastric mucosa and used the AGS cell culture model to determine the effects
of changes in SLP1 levels using SLP1-siRNA.
The study reports that progranulin is up-regulated during H. pylori infection,
however, SLP1 did not appear to have a regulatory role on progranulin in H.
pylori gastritis.
The methods used are appropriate and well described and the conclusions are
supported by the data presented in the paper.
The manuscript is well written, with only rare typos throughout the text, which
should be corrected.
The hypothesis regarding the possible role of SLP1 in progranulin regulation
could be better outlined by stating the hypothesis directly in the introduction and
abstract.
This is a descriptive study, that provides additive information regarding the host
response to H. pylori, suggesting a role for progranulins during H. pylori gastritis.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
'I declare that I have no competing interests'
Please review this paper.
user
Reviewer's report
Title:Mucosal Progranulin expression is induced by H. pylori, but independent of
Secretory Leukocyte Protease Inhibitor (SLPI) expression
Version: 1Date:24 April 2011
Reviewer: Antonia Sepulveda
Reviewer's report:
Progranulins are converted to granulins and are secreted by epithelial cells and
immune cells. Progranulin and granulins have been shown to regulate
inflammation, proliferation and wound healing. The authors characterized the
expression of progranulin and granulins in gastric mucosa (by measuring
progranulin) in relation to H. pylori status.
Further, since progranulin was previously shown to be regulated by SLP1 in
SLP1 deficient mice (SLP1 prevents progranulin degradation by elastase) the
authors hypothesized that progranulin levels could be correlated with SLP1 levels
in gastric mucosa and used the AGS cell culture model to determine the effects
of changes in SLP1 levels using SLP1-siRNA.
The study reports that progranulin is up-regulated during H. pylori infection,
however, SLP1 did not appear to have a regulatory role on progranulin in H.
pylori gastritis.
The methods used are appropriate and well described and the conclusions are
supported by the data presented in the paper.
The manuscript is well written, with only rare typos throughout the text, which
should be corrected.
The hypothesis regarding the possible role of SLP1 in progranulin regulation
could be better outlined by stating the hypothesis directly in the introduction and
abstract.
This is a descriptive study, that provides additive information regarding the host
response to H. pylori, suggesting a role for progranulins during H. pylori gastritis.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
'I declare that I have no competing interests'
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