Title: Prevalence and genotypes distribution of group A rotavirus among outpatient children
under five years with acute diarrhea in Shanghai, China, 2012-2018
Version: 3 Date: 16 Feb 2022
Reviewer's report:
1.Shanghai CDC has been carrying out similar monitoring, and some papers have been
published, for example#
Kuang X, Gong X, Zhang X, Pan H, Teng Z. Genetic diversity of group A rotavirus in
acute gastroenteritis outpatients in Shanghai from 2017 to 2018. BMC Infect Dis. 2020 Aug
12;20(1):596. doi: 10.1186/s12879-020-05279-x. PMID: 32787859; PMCID: PMC7425067.
2.It is necessary to explain clearly the sample collection method, such as how many samples
are taken every day, every week or every month. Different sampling methods affect the overall
detection rate, which also needs to be considered in the discussion.
3.In general, rotaviruses were first detected by real-time reverse transcription polymerase
chain reaction (RT-PCR) or ELISA to detect rotavirus, and  genotyping was performed using
semi-nested PCR . This paper directly uses semi-nested PCR to detect rotavirus, which may
underestimate the detection rate of rotavirus.
4.Detailed statistical methods should be stated.
5.The evolutionary analysis of major genes has not been carried out, which is a big limitation.
Title: Prevalence and genotypes distribution of group A rotavirus among outpatient children
under five years with acute diarrhea in Shanghai, China, 2012-2018
Version: 3 Date: 16 Feb 2022
Reviewer's report:
1.Shanghai CDC has been carrying out similar monitoring, and some papers have been
published, for example#
Kuang X, Gong X, Zhang X, Pan H, Teng Z. Genetic diversity of group A rotavirus in
acute gastroenteritis outpatients in Shanghai from 2017 to 2018. BMC Infect Dis. 2020 Aug
12;20(1):596. doi: 10.1186/s12879-020-05279-x. PMID: 32787859; PMCID: PMC7425067.
2.It is necessary to explain clearly the sample collection method, such as how many samples
are taken every day, every week or every month. Different sampling methods affect the overall
detection rate, which also needs to be considered in the discussion.
3.In general, rotaviruses were first detected by real-time reverse transcription polymerase
chain reaction (RT-PCR) or ELISA to detect rotavirus, and  genotyping was performed using
semi-nested PCR . This paper directly uses semi-nested PCR to detect rotavirus, which may
underestimate the detection rate of rotavirus.
4.Detailed statistical methods should be stated.
5.The evolutionary analysis of major genes has not been carried out, which is a big limitation.
Please review this paper.
user
Title: Prevalence and genotypes distribution of group A rotavirus among outpatient children
under five years with acute diarrhea in Shanghai, China, 2012-2018
Version: 3 Date: 16 Feb 2022
Reviewer's report:
1.Shanghai CDC has been carrying out similar monitoring, and some papers have been
published, for example#
Kuang X, Gong X, Zhang X, Pan H, Teng Z. Genetic diversity of group A rotavirus in
acute gastroenteritis outpatients in Shanghai from 2017 to 2018. BMC Infect Dis. 2020 Aug
12;20(1):596. doi: 10.1186/s12879-020-05279-x. PMID: 32787859; PMCID: PMC7425067.
2.It is necessary to explain clearly the sample collection method, such as how many samples
are taken every day, every week or every month. Different sampling methods affect the overall
detection rate, which also needs to be considered in the discussion.
3.In general, rotaviruses were first detected by real-time reverse transcription polymerase
chain reaction (RT-PCR) or ELISA to detect rotavirus, and  genotyping was performed using
semi-nested PCR . This paper directly uses semi-nested PCR to detect rotavirus, which may
underestimate the detection rate of rotavirus.
4.Detailed statistical methods should be stated.
5.The evolutionary analysis of major genes has not been carried out, which is a big limitation.
assistant