Reviewer's report
Title:Heterogeneity of Amplification of HER2, EGFR, CCND1 and MYC in
Gastric Cancer
Version:2Date:12 November 2014
Reviewer: Rocco Cappellesso
Reviewer's report:
In this study the authors dealt with intratumor heterogeneity in gastric cancer and
focused on HER2, EGFR, CCND1, and MYC amplification and HER2 protein
expression. The aim was well defined, the methods appropriate, the findings
interesting, images are good, and the discussion is properly addressed.However,
I have some minor concerns that the authors should address:
1. In the supplementary Table 1 the total number of cancers is 109 on the top of
the table but then they decrease to 94 or less. Control these incongruities.
2. The minimum number of nuclei for each spot that were evaluated should be
stated in FISH scoring.
3. A statistical analysis paragraph should be added to the methods.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
None to declare.
Reviewer's report
Title:Heterogeneity of Amplification of HER2, EGFR, CCND1 and MYC in
Gastric Cancer
Version:2Date:12 November 2014
Reviewer: Rocco Cappellesso
Reviewer's report:
In this study the authors dealt with intratumor heterogeneity in gastric cancer and
focused on HER2, EGFR, CCND1, and MYC amplification and HER2 protein
expression. The aim was well defined, the methods appropriate, the findings
interesting, images are good, and the discussion is properly addressed.However,
I have some minor concerns that the authors should address:
1. In the supplementary Table 1 the total number of cancers is 109 on the top of
the table but then they decrease to 94 or less. Control these incongruities.
2. The minimum number of nuclei for each spot that were evaluated should be
stated in FISH scoring.
3. A statistical analysis paragraph should be added to the methods.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
None to declare.
Please review this paper.
user
Reviewer's report
Title:Heterogeneity of Amplification of HER2, EGFR, CCND1 and MYC in
Gastric Cancer
Version:2Date:12 November 2014
Reviewer: Rocco Cappellesso
Reviewer's report:
In this study the authors dealt with intratumor heterogeneity in gastric cancer and
focused on HER2, EGFR, CCND1, and MYC amplification and HER2 protein
expression. The aim was well defined, the methods appropriate, the findings
interesting, images are good, and the discussion is properly addressed.However,
I have some minor concerns that the authors should address:
1. In the supplementary Table 1 the total number of cancers is 109 on the top of
the table but then they decrease to 94 or less. Control these incongruities.
2. The minimum number of nuclei for each spot that were evaluated should be
stated in FISH scoring.
3. A statistical analysis paragraph should be added to the methods.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
None to declare.
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