Title: 7-Hydroxycoumarin ameliorates ulcerative colitis in mice by inhibiting the MAPK
pathway and alleviating gut microbiota dysbiosis
Version: 5 Date: 29 Aug 2024
Reviewer's report:
In the manuscript titled "7-Hydroxycoumarin ameliorates ulcerative colitis in mice by inhibiting
the MAPK pathway and alleviating gut microbiota dysbiosis," the authors conclude that the
protective effect of 7-Hydroxycoumarin on DSS-induced ulcerative colitis in mice is mediated
through the inhibition of the MAPK signaling pathway in the colon and the restoration of
intestinal microbiota composition. However, before this manuscript can be considered for
publication, several critical issues need to be addressed to enhance the overall quality of the
study:
Consideration of NF-#B Activation: Since NF-#B is a crucial transcription factor involved in
the production of proinflammatory cytokines, it would be valuable to investigate the potential
effects of 7-Hydroxycoumarin on NF-#B activation.
Selection of Male Mice: The authors should clarify why only male mice were used in this study,
considering that sex differences can influence disease outcomes and drug efficacy.
Impact on Epithelial Barrier Function: Disruption of the epithelial barrier is a hallmark of
ulcerative colitis. The authors should examine whether 7-Hydroxycoumarin has any positive
effects on the expression or function of tight junction proteins.
Inclusion of Additional Colitis Models: To strengthen the findings, the study would benefit from
the inclusion of other colitis models beyond DSS-induced colitis, which would provide a broader
perspective on the therapeutic potential of 7-Hydroxycoumarin.
Improvement of Histological Images: The H&E images in Figure 1F need improvement to better
illustrate the effects of different doses of 7-Hydroxycoumarin on epithelial structure. The current
images primarily highlight the underlying lamina propria and submucosa rather than the crypt-
villus axis.
Title: 7-Hydroxycoumarin ameliorates ulcerative colitis in mice by inhibiting the MAPK
pathway and alleviating gut microbiota dysbiosis
Version: 5 Date: 29 Aug 2024
Reviewer's report:
In the manuscript titled "7-Hydroxycoumarin ameliorates ulcerative colitis in mice by inhibiting
the MAPK pathway and alleviating gut microbiota dysbiosis," the authors conclude that the
protective effect of 7-Hydroxycoumarin on DSS-induced ulcerative colitis in mice is mediated
through the inhibition of the MAPK signaling pathway in the colon and the restoration of
intestinal microbiota composition. However, before this manuscript can be considered for
publication, several critical issues need to be addressed to enhance the overall quality of the
study:
Consideration of NF-#B Activation: Since NF-#B is a crucial transcription factor involved in
the production of proinflammatory cytokines, it would be valuable to investigate the potential
effects of 7-Hydroxycoumarin on NF-#B activation.
Selection of Male Mice: The authors should clarify why only male mice were used in this study,
considering that sex differences can influence disease outcomes and drug efficacy.
Impact on Epithelial Barrier Function: Disruption of the epithelial barrier is a hallmark of
ulcerative colitis. The authors should examine whether 7-Hydroxycoumarin has any positive
effects on the expression or function of tight junction proteins.
Inclusion of Additional Colitis Models: To strengthen the findings, the study would benefit from
the inclusion of other colitis models beyond DSS-induced colitis, which would provide a broader
perspective on the therapeutic potential of 7-Hydroxycoumarin.
Improvement of Histological Images: The H&E images in Figure 1F need improvement to better
illustrate the effects of different doses of 7-Hydroxycoumarin on epithelial structure. The current
images primarily highlight the underlying lamina propria and submucosa rather than the crypt-
villus axis.
Please review this paper.
user
Title: 7-Hydroxycoumarin ameliorates ulcerative colitis in mice by inhibiting the MAPK
pathway and alleviating gut microbiota dysbiosis
Version: 5 Date: 29 Aug 2024
Reviewer's report:
In the manuscript titled "7-Hydroxycoumarin ameliorates ulcerative colitis in mice by inhibiting
the MAPK pathway and alleviating gut microbiota dysbiosis," the authors conclude that the
protective effect of 7-Hydroxycoumarin on DSS-induced ulcerative colitis in mice is mediated
through the inhibition of the MAPK signaling pathway in the colon and the restoration of
intestinal microbiota composition. However, before this manuscript can be considered for
publication, several critical issues need to be addressed to enhance the overall quality of the
study:
Consideration of NF-#B Activation: Since NF-#B is a crucial transcription factor involved in
the production of proinflammatory cytokines, it would be valuable to investigate the potential
effects of 7-Hydroxycoumarin on NF-#B activation.
Selection of Male Mice: The authors should clarify why only male mice were used in this study,
considering that sex differences can influence disease outcomes and drug efficacy.
Impact on Epithelial Barrier Function: Disruption of the epithelial barrier is a hallmark of
ulcerative colitis. The authors should examine whether 7-Hydroxycoumarin has any positive
effects on the expression or function of tight junction proteins.
Inclusion of Additional Colitis Models: To strengthen the findings, the study would benefit from
the inclusion of other colitis models beyond DSS-induced colitis, which would provide a broader
perspective on the therapeutic potential of 7-Hydroxycoumarin.
Improvement of Histological Images: The H&E images in Figure 1F need improvement to better
illustrate the effects of different doses of 7-Hydroxycoumarin on epithelial structure. The current
images primarily highlight the underlying lamina propria and submucosa rather than the crypt-
villus axis.
assistant