Reviewer's report
Title:Influence of acute pancreatitis on the responsiveness of rat mesenteric and
pulmonary arteries
Version: 1Date:21 February 2008
Reviewer: Werner Hartwig
Reviewer's report:
Camargo et al. investigated the responsiveness of rat mesenteric and pulmonary
arteries in acute pancreatitis. In an experimental design they isolated these
vessels and performed in vitro experiments using acetylcholine, sodium
nitroprussid and phenylephrine to assess vascular reactivity. The authors
conclude that acute pancreatitis induces endothelium-dependent changes in
relaxation/contractile response in these vessels which might be involved in the
pathogenesis of systemic organ injury.
Overall, the manuscript is well written. However, the reviewer would suggest
some improvements:
1. Arterial endothelium damage has been suggested to be responsible for the
observed findings. It would be helpful if the authors could provide more evidence
to support this hypothesis (e.g. electron microscopy). Since all measurements of
vascular reactivity have been performed in vitro, it may also help to assess
vascular changes in vivo (e.g. mesenteric vessels are well accessible for
intravital microscopy).
2. Changes have been presented only for the very early course of acute
pancreatitis (4 hours). Since the maximum expression of inducible NOS is at
about 6-12 hours after induction of acute pancreatitis, the reviewer suggests
additional experiments at some later point to show possible changes also in
pulmonary arteries.
3. Do the findings correlate with the severity of organ injury in these models of
acute pancreatitis?
Minors:
Methods: Please provide the amount of animals used for each experiment.
Furthermore, data should be presented characterizing the severity of the disease
in each experimental group.
Some spelling errors, e.g.:
Page 3: â#¦have showedâ#¦
Page 6: â#¦.proinflammatory cytokines is â#¦
Page 8: â#¦cut into to ringsâ#¦.
Figure 1: no significances provided (but in figure legends)
Table 1: just maximal relaxation response presented. Somewhat confusing with
phenylephrine
What next?: Unable to decide on acceptance or rejection until the authors have
responded to the major compulsory revisions
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: Yes, and I have assessed the statistics in my report.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Influence of acute pancreatitis on the responsiveness of rat mesenteric and
pulmonary arteries
Version: 1Date:21 February 2008
Reviewer: Werner Hartwig
Reviewer's report:
Camargo et al. investigated the responsiveness of rat mesenteric and pulmonary
arteries in acute pancreatitis. In an experimental design they isolated these
vessels and performed in vitro experiments using acetylcholine, sodium
nitroprussid and phenylephrine to assess vascular reactivity. The authors
conclude that acute pancreatitis induces endothelium-dependent changes in
relaxation/contractile response in these vessels which might be involved in the
pathogenesis of systemic organ injury.
Overall, the manuscript is well written. However, the reviewer would suggest
some improvements:
1. Arterial endothelium damage has been suggested to be responsible for the
observed findings. It would be helpful if the authors could provide more evidence
to support this hypothesis (e.g. electron microscopy). Since all measurements of
vascular reactivity have been performed in vitro, it may also help to assess
vascular changes in vivo (e.g. mesenteric vessels are well accessible for
intravital microscopy).
2. Changes have been presented only for the very early course of acute
pancreatitis (4 hours). Since the maximum expression of inducible NOS is at
about 6-12 hours after induction of acute pancreatitis, the reviewer suggests
additional experiments at some later point to show possible changes also in
pulmonary arteries.
3. Do the findings correlate with the severity of organ injury in these models of
acute pancreatitis?
Minors:
Methods: Please provide the amount of animals used for each experiment.
Furthermore, data should be presented characterizing the severity of the disease
in each experimental group.
Some spelling errors, e.g.:
Page 3: â#¦have showedâ#¦
Page 6: â#¦.proinflammatory cytokines is â#¦
Page 8: â#¦cut into to ringsâ#¦.
Figure 1: no significances provided (but in figure legends)
Table 1: just maximal relaxation response presented. Somewhat confusing with
phenylephrine
What next?: Unable to decide on acceptance or rejection until the authors have
responded to the major compulsory revisions
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: Yes, and I have assessed the statistics in my report.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Influence of acute pancreatitis on the responsiveness of rat mesenteric and
pulmonary arteries
Version: 1Date:21 February 2008
Reviewer: Werner Hartwig
Reviewer's report:
Camargo et al. investigated the responsiveness of rat mesenteric and pulmonary
arteries in acute pancreatitis. In an experimental design they isolated these
vessels and performed in vitro experiments using acetylcholine, sodium
nitroprussid and phenylephrine to assess vascular reactivity. The authors
conclude that acute pancreatitis induces endothelium-dependent changes in
relaxation/contractile response in these vessels which might be involved in the
pathogenesis of systemic organ injury.
Overall, the manuscript is well written. However, the reviewer would suggest
some improvements:
1. Arterial endothelium damage has been suggested to be responsible for the
observed findings. It would be helpful if the authors could provide more evidence
to support this hypothesis (e.g. electron microscopy). Since all measurements of
vascular reactivity have been performed in vitro, it may also help to assess
vascular changes in vivo (e.g. mesenteric vessels are well accessible for
intravital microscopy).
2. Changes have been presented only for the very early course of acute
pancreatitis (4 hours). Since the maximum expression of inducible NOS is at
about 6-12 hours after induction of acute pancreatitis, the reviewer suggests
additional experiments at some later point to show possible changes also in
pulmonary arteries.
3. Do the findings correlate with the severity of organ injury in these models of
acute pancreatitis?
Minors:
Methods: Please provide the amount of animals used for each experiment.
Furthermore, data should be presented characterizing the severity of the disease
in each experimental group.
Some spelling errors, e.g.:
Page 3: â#¦have showedâ#¦
Page 6: â#¦.proinflammatory cytokines is â#¦
Page 8: â#¦cut into to ringsâ#¦.
Figure 1: no significances provided (but in figure legends)
Table 1: just maximal relaxation response presented. Somewhat confusing with
phenylephrine
What next?: Unable to decide on acceptance or rejection until the authors have
responded to the major compulsory revisions
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: Yes, and I have assessed the statistics in my report.
Declaration of competing interests:
I declare that I have no competing interests
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