Title: RFC2 promotes aerobic glycolysis and progression of colorectal cancer
Version: 1 Date: 11 Jul 2023
Reviewer's report:
RFC2 was upregulated in CRCs and associated with poor prognosis.
Knockdown of RFC2 inhibited CRC cell proliferation, induced apoptosis, and suppressed
migration and invasion; knockdown of CREB5 suppressed RFC2 expression. Furthermore,
RFC2 promoted aerobic glycolysis and the PI3K/AKT/mTOR pathway.
Major questions
1. The underline mechanism of RFC2 in CRC is not fully investigated. What are some of the
things reported in relation to RFC1-5 (except 2) and CRC? (P4, line 57)
2. Higher expression of RFC2 predicted poor prognosis of CRC. I would like to know about the
relationship between RFC2 and prognosis at your institution. (P7, line 152)
3. Why did you use SW480, HCT116 when evaluating proliferation, etc.? I think you need to
describe the expression of RFC2 with other cells.
4. I would like to know more about the relationship between CREB5 in the MET pathway and
which part of RFC2 affects it.
Minor questions
1. I think it would be better to make it clearer which is the tumor in Fig. 1-A.
2. I think it should be noted that a significant difference was found in Fig1-B.
3. What are the numbers 1-8 in Fig. 1-D?
4. I think #, &, etc. in Fig3 need to be explained.
Title: RFC2 promotes aerobic glycolysis and progression of colorectal cancer
Version: 1 Date: 11 Jul 2023
Reviewer's report:
RFC2 was upregulated in CRCs and associated with poor prognosis.
Knockdown of RFC2 inhibited CRC cell proliferation, induced apoptosis, and suppressed
migration and invasion; knockdown of CREB5 suppressed RFC2 expression. Furthermore,
RFC2 promoted aerobic glycolysis and the PI3K/AKT/mTOR pathway.
Major questions
1. The underline mechanism of RFC2 in CRC is not fully investigated. What are some of the
things reported in relation to RFC1-5 (except 2) and CRC? (P4, line 57)
2. Higher expression of RFC2 predicted poor prognosis of CRC. I would like to know about the
relationship between RFC2 and prognosis at your institution. (P7, line 152)
3. Why did you use SW480, HCT116 when evaluating proliferation, etc.? I think you need to
describe the expression of RFC2 with other cells.
4. I would like to know more about the relationship between CREB5 in the MET pathway and
which part of RFC2 affects it.
Minor questions
1. I think it would be better to make it clearer which is the tumor in Fig. 1-A.
2. I think it should be noted that a significant difference was found in Fig1-B.
3. What are the numbers 1-8 in Fig. 1-D?
4. I think #, &, etc. in Fig3 need to be explained.
Please review this paper.
user
Title: RFC2 promotes aerobic glycolysis and progression of colorectal cancer
Version: 1 Date: 11 Jul 2023
Reviewer's report:
RFC2 was upregulated in CRCs and associated with poor prognosis.
Knockdown of RFC2 inhibited CRC cell proliferation, induced apoptosis, and suppressed
migration and invasion; knockdown of CREB5 suppressed RFC2 expression. Furthermore,
RFC2 promoted aerobic glycolysis and the PI3K/AKT/mTOR pathway.
Major questions
1. The underline mechanism of RFC2 in CRC is not fully investigated. What are some of the
things reported in relation to RFC1-5 (except 2) and CRC? (P4, line 57)
2. Higher expression of RFC2 predicted poor prognosis of CRC. I would like to know about the
relationship between RFC2 and prognosis at your institution. (P7, line 152)
3. Why did you use SW480, HCT116 when evaluating proliferation, etc.? I think you need to
describe the expression of RFC2 with other cells.
4. I would like to know more about the relationship between CREB5 in the MET pathway and
which part of RFC2 affects it.
Minor questions
1. I think it would be better to make it clearer which is the tumor in Fig. 1-A.
2. I think it should be noted that a significant difference was found in Fig1-B.
3. What are the numbers 1-8 in Fig. 1-D?
4. I think #, &, etc. in Fig3 need to be explained.
assistant