Title: PTEN-induced kinase 1 gene single-nucleotide variants as biomarkers in adjuvant
chemotherapy for colorectal cancer: a retrospective study
Version: 1 Date: 23 Jul 2023
Reviewer's report:
PTEN-induced kinase 1 gene single-nucleotide variants as biomarkers in adjuvant chemotherapy
for colorectal cancer
Journal Name: BMC Gastroenterology
This study aimed to find an early prognostic marker for CRC disease recurrence through deep
sequencing analysis for some autophagy related genes. In the 84 analyzed specimens, they found
single-nucleotide variants c.1018G>A or c.1562A>C of the mitophagy-related gene PTEN-
induced kinase 1 to be significantly associated with the low rate of recurrence in patients treated
with 5-FU based therapy. I summarized my comments:
1. Please provide legend each table in the main text or in the supplementary describing the data
presented, any abbreviations, and statistical analysis used to measure the significance.
2. Please present data as count and % not count only.
3. Please write at the end of the Material and Methods section; the statistical procedure in detail
and the survival measurement how it is calculated?
4. How recurrence was evaluated (radio, pathology, clinical …), is it local or distant, and what
were the main sites of recurrence?
5. How long did the treatment with 5-FU based therapy last? Was there any discontinuation, or
signs of toxicity in patients with the detected mutations?
6. The results showed significant differences only for the mutation in PINK1 to be associated
with the non-recurrence of CRC disease, suggesting to cause low expression in the transcripts
and proteins, is there a chance to test that in patient`s tissue samples with the appropriate
antibodies via immunohistochemistry?
Title: PTEN-induced kinase 1 gene single-nucleotide variants as biomarkers in adjuvant
chemotherapy for colorectal cancer: a retrospective study
Version: 1 Date: 23 Jul 2023
Reviewer's report:
PTEN-induced kinase 1 gene single-nucleotide variants as biomarkers in adjuvant chemotherapy
for colorectal cancer
Journal Name: BMC Gastroenterology
This study aimed to find an early prognostic marker for CRC disease recurrence through deep
sequencing analysis for some autophagy related genes. In the 84 analyzed specimens, they found
single-nucleotide variants c.1018G>A or c.1562A>C of the mitophagy-related gene PTEN-
induced kinase 1 to be significantly associated with the low rate of recurrence in patients treated
with 5-FU based therapy. I summarized my comments:
1. Please provide legend each table in the main text or in the supplementary describing the data
presented, any abbreviations, and statistical analysis used to measure the significance.
2. Please present data as count and % not count only.
3. Please write at the end of the Material and Methods section; the statistical procedure in detail
and the survival measurement how it is calculated?
4. How recurrence was evaluated (radio, pathology, clinical …), is it local or distant, and what
were the main sites of recurrence?
5. How long did the treatment with 5-FU based therapy last? Was there any discontinuation, or
signs of toxicity in patients with the detected mutations?
6. The results showed significant differences only for the mutation in PINK1 to be associated
with the non-recurrence of CRC disease, suggesting to cause low expression in the transcripts
and proteins, is there a chance to test that in patient`s tissue samples with the appropriate
antibodies via immunohistochemistry?
Please review this paper.
user
Title: PTEN-induced kinase 1 gene single-nucleotide variants as biomarkers in adjuvant
chemotherapy for colorectal cancer: a retrospective study
Version: 1 Date: 23 Jul 2023
Reviewer's report:
PTEN-induced kinase 1 gene single-nucleotide variants as biomarkers in adjuvant chemotherapy
for colorectal cancer
Journal Name: BMC Gastroenterology
This study aimed to find an early prognostic marker for CRC disease recurrence through deep
sequencing analysis for some autophagy related genes. In the 84 analyzed specimens, they found
single-nucleotide variants c.1018G>A or c.1562A>C of the mitophagy-related gene PTEN-
induced kinase 1 to be significantly associated with the low rate of recurrence in patients treated
with 5-FU based therapy. I summarized my comments:
1. Please provide legend each table in the main text or in the supplementary describing the data
presented, any abbreviations, and statistical analysis used to measure the significance.
2. Please present data as count and % not count only.
3. Please write at the end of the Material and Methods section; the statistical procedure in detail
and the survival measurement how it is calculated?
4. How recurrence was evaluated (radio, pathology, clinical …), is it local or distant, and what
were the main sites of recurrence?
5. How long did the treatment with 5-FU based therapy last? Was there any discontinuation, or
signs of toxicity in patients with the detected mutations?
6. The results showed significant differences only for the mutation in PINK1 to be associated
with the non-recurrence of CRC disease, suggesting to cause low expression in the transcripts
and proteins, is there a chance to test that in patient`s tissue samples with the appropriate
antibodies via immunohistochemistry?
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