Reviewer's report
Title:Comparative analysis of inflamed and non-inflamed colon biopsies reveals
strong proteomic inflammation profile in patients with ulcerative colitis
Version: 3Date:4 June 2012
Reviewer: Ryan Stidham
Reviewer's report:
Prior Major Revision Suggestions:
1. Clearly state in the abstract and background that the purpose of this work is to
describe the proteomic profile in ulcerative colitis, not the express discovery of
new biomarkers: The authors have altered the text to better characterize their
conclusions.
2. Narrow the UC cohort to identical medical therapy (all 5-ASA, all treatment
naïve, etc) including non-IBD medications and reanalyze the data. This should be
possible given the small number of patients necessary to enroll: Appreciate
author's thoughtful response, and agree that they present reasonable justification
to conclude that variation in 5-ASA use did not impact results.
3. Add a non-IBD inflammation control group (i.e. C diff colitis distal colon
biopsies) for comparison and clarification of proteins unique to IBD related
inflammation: I still suggest a Non-IBD colitis positive control. However, this will
not be practical for the authors and their results and conclusion are a valuable
addition to IBD-related proteomic research.
4. Please provide histology inflammation grading for UC cohort: Sufficient
clarification.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests' below. If your reply is yes to any,
please give details below.
Reviewer's report
Title:Comparative analysis of inflamed and non-inflamed colon biopsies reveals
strong proteomic inflammation profile in patients with ulcerative colitis
Version: 3Date:4 June 2012
Reviewer: Ryan Stidham
Reviewer's report:
Prior Major Revision Suggestions:
1. Clearly state in the abstract and background that the purpose of this work is to
describe the proteomic profile in ulcerative colitis, not the express discovery of
new biomarkers: The authors have altered the text to better characterize their
conclusions.
2. Narrow the UC cohort to identical medical therapy (all 5-ASA, all treatment
naïve, etc) including non-IBD medications and reanalyze the data. This should be
possible given the small number of patients necessary to enroll: Appreciate
author's thoughtful response, and agree that they present reasonable justification
to conclude that variation in 5-ASA use did not impact results.
3. Add a non-IBD inflammation control group (i.e. C diff colitis distal colon
biopsies) for comparison and clarification of proteins unique to IBD related
inflammation: I still suggest a Non-IBD colitis positive control. However, this will
not be practical for the authors and their results and conclusion are a valuable
addition to IBD-related proteomic research.
4. Please provide histology inflammation grading for UC cohort: Sufficient
clarification.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests' below. If your reply is yes to any,
please give details below.
Please review this paper.
user
Reviewer's report
Title:Comparative analysis of inflamed and non-inflamed colon biopsies reveals
strong proteomic inflammation profile in patients with ulcerative colitis
Version: 3Date:4 June 2012
Reviewer: Ryan Stidham
Reviewer's report:
Prior Major Revision Suggestions:
1. Clearly state in the abstract and background that the purpose of this work is to
describe the proteomic profile in ulcerative colitis, not the express discovery of
new biomarkers: The authors have altered the text to better characterize their
conclusions.
2. Narrow the UC cohort to identical medical therapy (all 5-ASA, all treatment
naïve, etc) including non-IBD medications and reanalyze the data. This should be
possible given the small number of patients necessary to enroll: Appreciate
author's thoughtful response, and agree that they present reasonable justification
to conclude that variation in 5-ASA use did not impact results.
3. Add a non-IBD inflammation control group (i.e. C diff colitis distal colon
biopsies) for comparison and clarification of proteins unique to IBD related
inflammation: I still suggest a Non-IBD colitis positive control. However, this will
not be practical for the authors and their results and conclusion are a valuable
addition to IBD-related proteomic research.
4. Please provide histology inflammation grading for UC cohort: Sufficient
clarification.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests' below. If your reply is yes to any,
please give details below.
assistant