Reviewer's report
Title:Fluorescence imaging in vivo visualizes delayed gastric emptying of liquid
enteral nutrition containing pectin
Version:2Date:28 May 2014
Reviewer: James Hollis
Reviewer's report:
Fluorescence imaging in vivo visualizes delayed gastric emptying of liquid enteral
nutrition containing pectin
This paper describes an innovative method for measuring gastric emptying in
mice. It is applied to determine the gastric emptying rate of a novel pectin based
enteral nutrition product. As expected, the addition of pectin to the bolus
increased viscosity in simulated gastric environment. However, a viscosity
measurement was only conducted at one speed on the viscometer and it is not
clear how the material would respond at other speeds (e.g., is it sheer thinning or
sheer thickening) (discretionary revision – may be worth mentioning in
discussion). The bolus with the added pectin reduced gastric emptying rate as
indicated by the florescence imaging. While this result would not be unexpected
the use of the florescence imaging to measure the gastric emptying rate makes it
of interest.
The paper is generally well written and easy to follow. The paper is brief and the
results may be limited in scope. Moreover, I feel the authors should discuss the
limitations of this approach in a little more depth (compulsory revision). However,
I feel this is a worthwhile addition to the literature.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Fluorescence imaging in vivo visualizes delayed gastric emptying of liquid
enteral nutrition containing pectin
Version:2Date:28 May 2014
Reviewer: James Hollis
Reviewer's report:
Fluorescence imaging in vivo visualizes delayed gastric emptying of liquid enteral
nutrition containing pectin
This paper describes an innovative method for measuring gastric emptying in
mice. It is applied to determine the gastric emptying rate of a novel pectin based
enteral nutrition product. As expected, the addition of pectin to the bolus
increased viscosity in simulated gastric environment. However, a viscosity
measurement was only conducted at one speed on the viscometer and it is not
clear how the material would respond at other speeds (e.g., is it sheer thinning or
sheer thickening) (discretionary revision – may be worth mentioning in
discussion). The bolus with the added pectin reduced gastric emptying rate as
indicated by the florescence imaging. While this result would not be unexpected
the use of the florescence imaging to measure the gastric emptying rate makes it
of interest.
The paper is generally well written and easy to follow. The paper is brief and the
results may be limited in scope. Moreover, I feel the authors should discuss the
limitations of this approach in a little more depth (compulsory revision). However,
I feel this is a worthwhile addition to the literature.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Fluorescence imaging in vivo visualizes delayed gastric emptying of liquid
enteral nutrition containing pectin
Version:2Date:28 May 2014
Reviewer: James Hollis
Reviewer's report:
Fluorescence imaging in vivo visualizes delayed gastric emptying of liquid enteral
nutrition containing pectin
This paper describes an innovative method for measuring gastric emptying in
mice. It is applied to determine the gastric emptying rate of a novel pectin based
enteral nutrition product. As expected, the addition of pectin to the bolus
increased viscosity in simulated gastric environment. However, a viscosity
measurement was only conducted at one speed on the viscometer and it is not
clear how the material would respond at other speeds (e.g., is it sheer thinning or
sheer thickening) (discretionary revision – may be worth mentioning in
discussion). The bolus with the added pectin reduced gastric emptying rate as
indicated by the florescence imaging. While this result would not be unexpected
the use of the florescence imaging to measure the gastric emptying rate makes it
of interest.
The paper is generally well written and easy to follow. The paper is brief and the
results may be limited in scope. Moreover, I feel the authors should discuss the
limitations of this approach in a little more depth (compulsory revision). However,
I feel this is a worthwhile addition to the literature.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I declare that I have no competing interests
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