Reviewer’s report
Title: 
Gene Expression Profiling of Inflammatory Cytokines in Esophageal
Biopsies of Different Phenotypes of Gastroesophageal Reflux Disease: a cross-
sectional study
Version: 
0 
Date: 
01 May 2020
Reviewer: 
Peter W Dettmar
Reviewer's report:
Please include all comments for the authors in this box rather than uploading
your report as an attachment. Please only upload as attachments annotated
versions of manuscripts, graphs, supporting materials or other aspects of your
report which cannot be included in a text format.
Please overwrite this text when adding your comments to the authors.
The study has been well designed and the data generated interesting and well
presented. 
There are a few minor comments which need addressing. These are as follows:
1. Page 5 line 99 - Define GWAS 
2. Page 11 line 241- remove 
the words in a 
3. Page 11 line 254 - should be show not showed
4. Page 13 line 294 - remove the word in should read "with EE compared"
5. Page 13 line 294 - add the word the should read "with the controls"
6. Page 13 line 304 - remove the word in should read "MMP-9 compared"
7.Page 13 line 304 - add the word the should read "with the control group"
8. Page 13 line 310 - add the word it should read "in this study it is important"
9. Page 14 line 315 - add the word in should read "increase in the expression"
10. Page 14 line 324 - first word should be personalized medicine 
11, Page 14 line 331 - should read reversing the alteration of these genes. 
The above are minor corrections which need correcting.
Are the methods appropriate and well described?
If not, please specify what is required in your comments to the authors.
Yes
Does the work include the necessary controls?
If not, please specify which controls are required in your comments to the
authors.
Yes
Are the conclusions drawn adequately supported by the data shown?
If not, please explain in your comments to the authors.
Yes
Are you able to assess any statistics in the manuscript or would you
recommend an additional statistical review?
If an additional statistical review is recommended, please specify what aspects
require further assessment in your comments to the editors.
I am able to assess the statistics
Quality of written English
Please indicate the quality of language in the manuscript:
Acceptable
Declaration of competing interests
Please complete a declaration of competing interests, considering the following
questions:
1
. 
Have you in the past five years received reimbursements, fees, funding, or
salary from an organisation that may in any way gain or lose financially
from the publication of this manuscript, either now or in the future?
2
. 
Do you hold any stocks or shares in an organisation that may in any way
gain or lose financially from the publication of this manuscript, either now
or in the future?
3
. 
Do you hold or are you currently applying for any patents relating to the
content of the manuscript?
4
. 
Have you received reimbursements, fees, funding, or salary from an
organization that holds or has applied for patents relating to the content of
the manuscript?
5
. 
Do you have any other financial competing interests?
6
. 
Do you have any non-financial competing interests in relation to this
paper?
If you can answer no to all of the above, write 'I declare that I have no competing
interests' below. If your reply is yes to any, please give details below.
"I declare that I have no competing interests"
Reviewer Publication Consent.
 I agree for my report to be made available
under an Open Access Creative Commons CC-BY License (
http://creativecommons.org/licenses/by/4.0
) if this manuscript is accepted for
publication. Any comments that I do not wish to be included in the published
report have been included as confidential comments to the editor, which will not
be published.
If you are not happy for us to publish this report, please contact the editorial
office before completing the review.
If you wish, you can include your name in your published report. Please note
you must decide whether to include your name at the start of the process and
confirm this decision whenever prompted. If you change your decision later, we
will not be able to publish your name.
I agree to the terms of the CC-BY 4.0 license; please publish my name with my
report.
Reviewer’s report
Title: 
Gene Expression Profiling of Inflammatory Cytokines in Esophageal
Biopsies of Different Phenotypes of Gastroesophageal Reflux Disease: a cross-
sectional study
Version: 
0 
Date: 
01 May 2020
Reviewer: 
Peter W Dettmar
Reviewer's report:
Please include all comments for the authors in this box rather than uploading
your report as an attachment. Please only upload as attachments annotated
versions of manuscripts, graphs, supporting materials or other aspects of your
report which cannot be included in a text format.
Please overwrite this text when adding your comments to the authors.
The study has been well designed and the data generated interesting and well
presented. 
There are a few minor comments which need addressing. These are as follows:
1. Page 5 line 99 - Define GWAS 
2. Page 11 line 241- remove 
the words in a 
3. Page 11 line 254 - should be show not showed
4. Page 13 line 294 - remove the word in should read "with EE compared"
5. Page 13 line 294 - add the word the should read "with the controls"
6. Page 13 line 304 - remove the word in should read "MMP-9 compared"
7.Page 13 line 304 - add the word the should read "with the control group"
8. Page 13 line 310 - add the word it should read "in this study it is important"
9. Page 14 line 315 - add the word in should read "increase in the expression"
10. Page 14 line 324 - first word should be personalized medicine 
11, Page 14 line 331 - should read reversing the alteration of these genes. 
The above are minor corrections which need correcting.
Are the methods appropriate and well described?
If not, please specify what is required in your comments to the authors.
Yes
Does the work include the necessary controls?
If not, please specify which controls are required in your comments to the
authors.
Yes
Are the conclusions drawn adequately supported by the data shown?
If not, please explain in your comments to the authors.
Yes
Are you able to assess any statistics in the manuscript or would you
recommend an additional statistical review?
If an additional statistical review is recommended, please specify what aspects
require further assessment in your comments to the editors.
I am able to assess the statistics
Quality of written English
Please indicate the quality of language in the manuscript:
Acceptable
Declaration of competing interests
Please complete a declaration of competing interests, considering the following
questions:
1
. 
Have you in the past five years received reimbursements, fees, funding, or
salary from an organisation that may in any way gain or lose financially
from the publication of this manuscript, either now or in the future?
2
. 
Do you hold any stocks or shares in an organisation that may in any way
gain or lose financially from the publication of this manuscript, either now
or in the future?
3
. 
Do you hold or are you currently applying for any patents relating to the
content of the manuscript?
4
. 
Have you received reimbursements, fees, funding, or salary from an
organization that holds or has applied for patents relating to the content of
the manuscript?
5
. 
Do you have any other financial competing interests?
6
. 
Do you have any non-financial competing interests in relation to this
paper?
If you can answer no to all of the above, write 'I declare that I have no competing
interests' below. If your reply is yes to any, please give details below.
"I declare that I have no competing interests"
Reviewer Publication Consent.
 I agree for my report to be made available
under an Open Access Creative Commons CC-BY License (
http://creativecommons.org/licenses/by/4.0
) if this manuscript is accepted for
publication. Any comments that I do not wish to be included in the published
report have been included as confidential comments to the editor, which will not
be published.
If you are not happy for us to publish this report, please contact the editorial
office before completing the review.
If you wish, you can include your name in your published report. Please note
you must decide whether to include your name at the start of the process and
confirm this decision whenever prompted. If you change your decision later, we
will not be able to publish your name.
I agree to the terms of the CC-BY 4.0 license; please publish my name with my
report.
Please review this paper.
user
Reviewer’s report
Title: 
Gene Expression Profiling of Inflammatory Cytokines in Esophageal
Biopsies of Different Phenotypes of Gastroesophageal Reflux Disease: a cross-
sectional study
Version: 
0 
Date: 
01 May 2020
Reviewer: 
Peter W Dettmar
Reviewer's report:
Please include all comments for the authors in this box rather than uploading
your report as an attachment. Please only upload as attachments annotated
versions of manuscripts, graphs, supporting materials or other aspects of your
report which cannot be included in a text format.
Please overwrite this text when adding your comments to the authors.
The study has been well designed and the data generated interesting and well
presented. 
There are a few minor comments which need addressing. These are as follows:
1. Page 5 line 99 - Define GWAS 
2. Page 11 line 241- remove 
the words in a 
3. Page 11 line 254 - should be show not showed
4. Page 13 line 294 - remove the word in should read "with EE compared"
5. Page 13 line 294 - add the word the should read "with the controls"
6. Page 13 line 304 - remove the word in should read "MMP-9 compared"
7.Page 13 line 304 - add the word the should read "with the control group"
8. Page 13 line 310 - add the word it should read "in this study it is important"
9. Page 14 line 315 - add the word in should read "increase in the expression"
10. Page 14 line 324 - first word should be personalized medicine 
11, Page 14 line 331 - should read reversing the alteration of these genes. 
The above are minor corrections which need correcting.
Are the methods appropriate and well described?
If not, please specify what is required in your comments to the authors.
Yes
Does the work include the necessary controls?
If not, please specify which controls are required in your comments to the
authors.
Yes
Are the conclusions drawn adequately supported by the data shown?
If not, please explain in your comments to the authors.
Yes
Are you able to assess any statistics in the manuscript or would you
recommend an additional statistical review?
If an additional statistical review is recommended, please specify what aspects
require further assessment in your comments to the editors.
I am able to assess the statistics
Quality of written English
Please indicate the quality of language in the manuscript:
Acceptable
Declaration of competing interests
Please complete a declaration of competing interests, considering the following
questions:
1
. 
Have you in the past five years received reimbursements, fees, funding, or
salary from an organisation that may in any way gain or lose financially
from the publication of this manuscript, either now or in the future?
2
. 
Do you hold any stocks or shares in an organisation that may in any way
gain or lose financially from the publication of this manuscript, either now
or in the future?
3
. 
Do you hold or are you currently applying for any patents relating to the
content of the manuscript?
4
. 
Have you received reimbursements, fees, funding, or salary from an
organization that holds or has applied for patents relating to the content of
the manuscript?
5
. 
Do you have any other financial competing interests?
6
. 
Do you have any non-financial competing interests in relation to this
paper?
If you can answer no to all of the above, write 'I declare that I have no competing
interests' below. If your reply is yes to any, please give details below.
"I declare that I have no competing interests"
Reviewer Publication Consent.
 I agree for my report to be made available
under an Open Access Creative Commons CC-BY License (
http://creativecommons.org/licenses/by/4.0
) if this manuscript is accepted for
publication. Any comments that I do not wish to be included in the published
report have been included as confidential comments to the editor, which will not
be published.
If you are not happy for us to publish this report, please contact the editorial
office before completing the review.
If you wish, you can include your name in your published report. Please note
you must decide whether to include your name at the start of the process and
confirm this decision whenever prompted. If you change your decision later, we
will not be able to publish your name.
I agree to the terms of the CC-BY 4.0 license; please publish my name with my
report.
assistant