Reviewer's report
Title:Chlamydia trachomatis antigens in enteroendocrine cells of the small
bowel in patients with irritable bowel syndrome
Version: 1Date:14 September 2009
Reviewer: Lena Öhman
Reviewer's report:
Review comments on
Chlamydia trachomatis antigens in enteroendocrine cells of the small bowel in
patients with irritable bowel syndrome.
The authors have investigated the presence of Chlamydia trachomatis in the
small bowel in patients with irritable bowel syndrome. They have performed
staining of jejunal biopsies, but also examined the presence of Chlamydia
trachomatis proteins using western blot and Chlamydia trachomatis DNA using
PCR analysis.
The authors report convincing data of Chlamydia trachomatis antigen presence
in the intestinal mucosa of IBS patients, whereas most healthy controls are
negative for the same antigen.
The study is design in sound, the number of patients is sufficient and data is well
presented. The paper is well written and the findings are clearly stated.
Discretionary revisions
I would prefer a table or figure demonstrating the main finding of the article
(number of C. trachomatis positive patients and controls, and statistics), which is
well described in the first section of “results”. This may very well be
complemented by the current figure 1, showing examples of specific stainings.
It is very difficult to see the arrows described in the figure legend (are there really
any?) in figure 1.
The word macrophages might perhaps be included in the title of the paper?
The word “MOMP” needs an explanation in the abstract.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
'I declare that I have no competing interests'
Reviewer's report
Title:Chlamydia trachomatis antigens in enteroendocrine cells of the small
bowel in patients with irritable bowel syndrome
Version: 1Date:14 September 2009
Reviewer: Lena Öhman
Reviewer's report:
Review comments on
Chlamydia trachomatis antigens in enteroendocrine cells of the small bowel in
patients with irritable bowel syndrome.
The authors have investigated the presence of Chlamydia trachomatis in the
small bowel in patients with irritable bowel syndrome. They have performed
staining of jejunal biopsies, but also examined the presence of Chlamydia
trachomatis proteins using western blot and Chlamydia trachomatis DNA using
PCR analysis.
The authors report convincing data of Chlamydia trachomatis antigen presence
in the intestinal mucosa of IBS patients, whereas most healthy controls are
negative for the same antigen.
The study is design in sound, the number of patients is sufficient and data is well
presented. The paper is well written and the findings are clearly stated.
Discretionary revisions
I would prefer a table or figure demonstrating the main finding of the article
(number of C. trachomatis positive patients and controls, and statistics), which is
well described in the first section of “results”. This may very well be
complemented by the current figure 1, showing examples of specific stainings.
It is very difficult to see the arrows described in the figure legend (are there really
any?) in figure 1.
The word macrophages might perhaps be included in the title of the paper?
The word “MOMP” needs an explanation in the abstract.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
'I declare that I have no competing interests'
Please review this paper.
user
Reviewer's report
Title:Chlamydia trachomatis antigens in enteroendocrine cells of the small
bowel in patients with irritable bowel syndrome
Version: 1Date:14 September 2009
Reviewer: Lena Öhman
Reviewer's report:
Review comments on
Chlamydia trachomatis antigens in enteroendocrine cells of the small bowel in
patients with irritable bowel syndrome.
The authors have investigated the presence of Chlamydia trachomatis in the
small bowel in patients with irritable bowel syndrome. They have performed
staining of jejunal biopsies, but also examined the presence of Chlamydia
trachomatis proteins using western blot and Chlamydia trachomatis DNA using
PCR analysis.
The authors report convincing data of Chlamydia trachomatis antigen presence
in the intestinal mucosa of IBS patients, whereas most healthy controls are
negative for the same antigen.
The study is design in sound, the number of patients is sufficient and data is well
presented. The paper is well written and the findings are clearly stated.
Discretionary revisions
I would prefer a table or figure demonstrating the main finding of the article
(number of C. trachomatis positive patients and controls, and statistics), which is
well described in the first section of “results”. This may very well be
complemented by the current figure 1, showing examples of specific stainings.
It is very difficult to see the arrows described in the figure legend (are there really
any?) in figure 1.
The word macrophages might perhaps be included in the title of the paper?
The word “MOMP” needs an explanation in the abstract.
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
'I declare that I have no competing interests'
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