Title: Correlation between Color Doppler Flow Pattern and Molecular Biology in Elderly
Patients with Colon Cancer
Version: 2 Date: 07 May 2023
Reviewer's report:
In this study, 76 elderly patients with colorectal cancer were included. The blood flow grade
and blood flow distribution type of tumor tissue were analyzed by CDFI, and the levels of
tumor-related cytokines in serum were detected by ELISA assay. Then the correlation between
cytokines and CDFI blood flow analysis was studied. This study has certain innovation and
scientific value, but there are still the following problems, it is recommended to make minor
revision:
1. The groups about gender, tumor length, tumor infiltration of serous layer and lymphatic
metastasis in the "content" of the first column of Table 1 should be placed below each item, not
on the right; Table 2 is the same, please modify.
2. What is the basis for choosing cytokines such as TNF-#, IL-6 and VEGF for research?
3. Only using binary logistic regression to show that TNF-#, IL-6 and VEGF are independent
risk factors for the death of elderly patients with colon cancer lacks rigor.
4. What is the clinical value of studying the correlation between CDFI blood flow grade, tumor
vascularity and different cytokines, and it should be explained.
5. All the cases were operated and diagnosed by doctors with different seniority. Since the
diagnosis of blood flow signal largely depends on subjective judgment, how to exclude the
influence of subjective factors?
6. The specific statistical methods are not fully elaborated in the "Materials and Methods"
section, such as the method used for the normality test is not mentioned.
7. Please attach the ethics approval document.
8. Statistical difference "P" and "t" of t test should be expressed in italics.
9. The language of the article needs further improvement.
Title: Correlation between Color Doppler Flow Pattern and Molecular Biology in Elderly
Patients with Colon Cancer
Version: 2 Date: 07 May 2023
Reviewer's report:
In this study, 76 elderly patients with colorectal cancer were included. The blood flow grade
and blood flow distribution type of tumor tissue were analyzed by CDFI, and the levels of
tumor-related cytokines in serum were detected by ELISA assay. Then the correlation between
cytokines and CDFI blood flow analysis was studied. This study has certain innovation and
scientific value, but there are still the following problems, it is recommended to make minor
revision:
1. The groups about gender, tumor length, tumor infiltration of serous layer and lymphatic
metastasis in the "content" of the first column of Table 1 should be placed below each item, not
on the right; Table 2 is the same, please modify.
2. What is the basis for choosing cytokines such as TNF-#, IL-6 and VEGF for research?
3. Only using binary logistic regression to show that TNF-#, IL-6 and VEGF are independent
risk factors for the death of elderly patients with colon cancer lacks rigor.
4. What is the clinical value of studying the correlation between CDFI blood flow grade, tumor
vascularity and different cytokines, and it should be explained.
5. All the cases were operated and diagnosed by doctors with different seniority. Since the
diagnosis of blood flow signal largely depends on subjective judgment, how to exclude the
influence of subjective factors?
6. The specific statistical methods are not fully elaborated in the "Materials and Methods"
section, such as the method used for the normality test is not mentioned.
7. Please attach the ethics approval document.
8. Statistical difference "P" and "t" of t test should be expressed in italics.
9. The language of the article needs further improvement.
Please review this paper.
user
Title: Correlation between Color Doppler Flow Pattern and Molecular Biology in Elderly
Patients with Colon Cancer
Version: 2 Date: 07 May 2023
Reviewer's report:
In this study, 76 elderly patients with colorectal cancer were included. The blood flow grade
and blood flow distribution type of tumor tissue were analyzed by CDFI, and the levels of
tumor-related cytokines in serum were detected by ELISA assay. Then the correlation between
cytokines and CDFI blood flow analysis was studied. This study has certain innovation and
scientific value, but there are still the following problems, it is recommended to make minor
revision:
1. The groups about gender, tumor length, tumor infiltration of serous layer and lymphatic
metastasis in the "content" of the first column of Table 1 should be placed below each item, not
on the right; Table 2 is the same, please modify.
2. What is the basis for choosing cytokines such as TNF-#, IL-6 and VEGF for research?
3. Only using binary logistic regression to show that TNF-#, IL-6 and VEGF are independent
risk factors for the death of elderly patients with colon cancer lacks rigor.
4. What is the clinical value of studying the correlation between CDFI blood flow grade, tumor
vascularity and different cytokines, and it should be explained.
5. All the cases were operated and diagnosed by doctors with different seniority. Since the
diagnosis of blood flow signal largely depends on subjective judgment, how to exclude the
influence of subjective factors?
6. The specific statistical methods are not fully elaborated in the "Materials and Methods"
section, such as the method used for the normality test is not mentioned.
7. Please attach the ethics approval document.
8. Statistical difference "P" and "t" of t test should be expressed in italics.
9. The language of the article needs further improvement.
assistant