Title: Clinical characteristics and longitudinal changes of patients with non-alcoholic fatty liver
disease in two decades: the NAGALA study
Version: 2 Date: 03 Jan 2021
Reviewer's report:
Thank you for submitting this paper. I here list a number of comments regarding the methods
of the study.
It is not clarified whether the study population could be considered as a primary or secondary
care population.
How were patients invited for the health chech-up? were they apparently healthy subjects and
was there any selection based on age? Where all consecutive patients invited to take part to the
study?
A diagnosis of liver disease was based on medical history or full liver screen blood tests?
How was the population distributed statistically.
Regarding the results of the study:
As fibrosis is the clinically significant feature on NAFLD, was there any result of non invasive
markers of fibrosis (even those based on blood tests?), if not, it should be listed among the
limitations of the study
It would be interesting to build a predisctive model for NAFLD using binary logistic regression.
This could be derived in the first (derivation) cohort and then tested in the second (validation)
cohort. This could allow  could compare the predictive value of risk factors in the 2 different
cohorts
Title: Clinical characteristics and longitudinal changes of patients with non-alcoholic fatty liver
disease in two decades: the NAGALA study
Version: 2 Date: 03 Jan 2021
Reviewer's report:
Thank you for submitting this paper. I here list a number of comments regarding the methods
of the study.
It is not clarified whether the study population could be considered as a primary or secondary
care population.
How were patients invited for the health chech-up? were they apparently healthy subjects and
was there any selection based on age? Where all consecutive patients invited to take part to the
study?
A diagnosis of liver disease was based on medical history or full liver screen blood tests?
How was the population distributed statistically.
Regarding the results of the study:
As fibrosis is the clinically significant feature on NAFLD, was there any result of non invasive
markers of fibrosis (even those based on blood tests?), if not, it should be listed among the
limitations of the study
It would be interesting to build a predisctive model for NAFLD using binary logistic regression.
This could be derived in the first (derivation) cohort and then tested in the second (validation)
cohort. This could allow  could compare the predictive value of risk factors in the 2 different
cohorts
Please review this paper.
user
Title: Clinical characteristics and longitudinal changes of patients with non-alcoholic fatty liver
disease in two decades: the NAGALA study
Version: 2 Date: 03 Jan 2021
Reviewer's report:
Thank you for submitting this paper. I here list a number of comments regarding the methods
of the study.
It is not clarified whether the study population could be considered as a primary or secondary
care population.
How were patients invited for the health chech-up? were they apparently healthy subjects and
was there any selection based on age? Where all consecutive patients invited to take part to the
study?
A diagnosis of liver disease was based on medical history or full liver screen blood tests?
How was the population distributed statistically.
Regarding the results of the study:
As fibrosis is the clinically significant feature on NAFLD, was there any result of non invasive
markers of fibrosis (even those based on blood tests?), if not, it should be listed among the
limitations of the study
It would be interesting to build a predisctive model for NAFLD using binary logistic regression.
This could be derived in the first (derivation) cohort and then tested in the second (validation)
cohort. This could allow  could compare the predictive value of risk factors in the 2 different
cohorts
assistant