Title: Neutrophil extracellular traps predict postoperative pulmonary complications in paediatric
patients undergoing parental liver transplantation
Version: 1 Date: 18 Jul 2022
Reviewer:  Takashi Matsusaki
Reviewer's report:
This manuscript was a prospective observational study in a single academic center regarding the
postoperative pulmonary complication after parental liver transplantation. This study was quite
interesting for future research. I have some question.
1. Their sample size was too small for their conclusion compare to other papers.
2. 47% of PPC was a little high compared to other reports. I feel their definition of PPC was so
different among other reports. How did they decide the definition of “PPC”?
3. I felt that there were a lot of factors regarding postoperative PPC such as preoperative factors
including massive ascites and intraoperative factors of transfusion amounts and fluid balance.
Postoperative pain control and inhaled oxygen concentration were also important related to PPC.
They didn’t analyze these factors by multivariate analysis.
4. The frequency of RDS after pediatric liver transplantation was so rare. What was the reason
of RDS?
5. In living donor liver transplantation, the cold ischemic time was so small. I felt that the effect
of ischemia-reperfusion injury was so small to consider higher serum level of NET components.
What factor did influence the higher level of NET components?
6. What did influence the measurement of serum level of NET components?
Title: Neutrophil extracellular traps predict postoperative pulmonary complications in paediatric
patients undergoing parental liver transplantation
Version: 1 Date: 18 Jul 2022
Reviewer:  Takashi Matsusaki
Reviewer's report:
This manuscript was a prospective observational study in a single academic center regarding the
postoperative pulmonary complication after parental liver transplantation. This study was quite
interesting for future research. I have some question.
1. Their sample size was too small for their conclusion compare to other papers.
2. 47% of PPC was a little high compared to other reports. I feel their definition of PPC was so
different among other reports. How did they decide the definition of “PPC”?
3. I felt that there were a lot of factors regarding postoperative PPC such as preoperative factors
including massive ascites and intraoperative factors of transfusion amounts and fluid balance.
Postoperative pain control and inhaled oxygen concentration were also important related to PPC.
They didn’t analyze these factors by multivariate analysis.
4. The frequency of RDS after pediatric liver transplantation was so rare. What was the reason
of RDS?
5. In living donor liver transplantation, the cold ischemic time was so small. I felt that the effect
of ischemia-reperfusion injury was so small to consider higher serum level of NET components.
What factor did influence the higher level of NET components?
6. What did influence the measurement of serum level of NET components?
Please review this paper.
user
Title: Neutrophil extracellular traps predict postoperative pulmonary complications in paediatric
patients undergoing parental liver transplantation
Version: 1 Date: 18 Jul 2022
Reviewer:  Takashi Matsusaki
Reviewer's report:
This manuscript was a prospective observational study in a single academic center regarding the
postoperative pulmonary complication after parental liver transplantation. This study was quite
interesting for future research. I have some question.
1. Their sample size was too small for their conclusion compare to other papers.
2. 47% of PPC was a little high compared to other reports. I feel their definition of PPC was so
different among other reports. How did they decide the definition of “PPC”?
3. I felt that there were a lot of factors regarding postoperative PPC such as preoperative factors
including massive ascites and intraoperative factors of transfusion amounts and fluid balance.
Postoperative pain control and inhaled oxygen concentration were also important related to PPC.
They didn’t analyze these factors by multivariate analysis.
4. The frequency of RDS after pediatric liver transplantation was so rare. What was the reason
of RDS?
5. In living donor liver transplantation, the cold ischemic time was so small. I felt that the effect
of ischemia-reperfusion injury was so small to consider higher serum level of NET components.
What factor did influence the higher level of NET components?
6. What did influence the measurement of serum level of NET components?
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