Title: Behavioral abnormalities in C57BL/6 mice with chronic ulcerative colitis induced by DSS
Version: 3 Date: 15 Feb 2023
Reviewer's report:
1)
In the method, “In the sucrose preference experiment, the consumption of purified water and
sucrose water was significantly reduced in the 3% DSS group.” Isn't it only sucrose water that
has decreased significantly? In the figure, purified water is indicated as n.s.. Which is correct?
Also in the discussion, it is stated that ''in the sucrose preference test, the pure water drinking
amount of the 3% DSS model group was slightly reduced while the sucrose water drinking, the
amount is greatly reduced.''
2)
In the method, the author describes "the sucrose preference experiment" as a method of
behavioral evaluations. However, in the discussion, it states that: In the 3% DSS model, the
reason for the decrease in the amount of sucrose consumed was considered to be pain due to
enteritis. In the 1.5% DSS model, despite behavioral changes other than "the sucrose preference
test," the amount of sucrose drinking was not reduced because the enteritis was mild. Again, isn't
the sugar water preference test assessing behavioral change as in the method? In other words,
in the 1.5% DSS model, why does sugar water consumption not decrease despite all behavioral
changes occurring except for the sugar water preference test? Or is there any literature that shows
it?
Title: Behavioral abnormalities in C57BL/6 mice with chronic ulcerative colitis induced by DSS
Version: 3 Date: 15 Feb 2023
Reviewer's report:
1)
In the method, “In the sucrose preference experiment, the consumption of purified water and
sucrose water was significantly reduced in the 3% DSS group.” Isn't it only sucrose water that
has decreased significantly? In the figure, purified water is indicated as n.s.. Which is correct?
Also in the discussion, it is stated that ''in the sucrose preference test, the pure water drinking
amount of the 3% DSS model group was slightly reduced while the sucrose water drinking, the
amount is greatly reduced.''
2)
In the method, the author describes "the sucrose preference experiment" as a method of
behavioral evaluations. However, in the discussion, it states that: In the 3% DSS model, the
reason for the decrease in the amount of sucrose consumed was considered to be pain due to
enteritis. In the 1.5% DSS model, despite behavioral changes other than "the sucrose preference
test," the amount of sucrose drinking was not reduced because the enteritis was mild. Again, isn't
the sugar water preference test assessing behavioral change as in the method? In other words,
in the 1.5% DSS model, why does sugar water consumption not decrease despite all behavioral
changes occurring except for the sugar water preference test? Or is there any literature that shows
it?
Please review this paper.
user
Title: Behavioral abnormalities in C57BL/6 mice with chronic ulcerative colitis induced by DSS
Version: 3 Date: 15 Feb 2023
Reviewer's report:
1)
In the method, “In the sucrose preference experiment, the consumption of purified water and
sucrose water was significantly reduced in the 3% DSS group.” Isn't it only sucrose water that
has decreased significantly? In the figure, purified water is indicated as n.s.. Which is correct?
Also in the discussion, it is stated that ''in the sucrose preference test, the pure water drinking
amount of the 3% DSS model group was slightly reduced while the sucrose water drinking, the
amount is greatly reduced.''
2)
In the method, the author describes "the sucrose preference experiment" as a method of
behavioral evaluations. However, in the discussion, it states that: In the 3% DSS model, the
reason for the decrease in the amount of sucrose consumed was considered to be pain due to
enteritis. In the 1.5% DSS model, despite behavioral changes other than "the sucrose preference
test," the amount of sucrose drinking was not reduced because the enteritis was mild. Again, isn't
the sugar water preference test assessing behavioral change as in the method? In other words,
in the 1.5% DSS model, why does sugar water consumption not decrease despite all behavioral
changes occurring except for the sugar water preference test? Or is there any literature that shows
it?
assistant