Reviewer's report
Title:Increased proportion of nitric oxide synthase immunoreactive neurons in
rat ileal myenteric ganglia after severe acute pancreatitis
Version: 6Date:20 July 2011
Reviewer: Colin R Anderson
Reviewer's report:
The paper has been largely revised along the lines I requested. However, I
requested three compulsory revisions that have not been made, nor have the
authors responded to the requests. First, I asked whether the Hu neurons
changed in number as this is the only way that the reader can know whether
NOS neurons have increased in number versus whether Hu neurons have
decreased. This has not been answered, but I note that the authors now say they
have counted only 200 Hu neurons at a time, so this implies they don't have the
data to answer my original request. Therefore, I would simply ask that they point
out the impossibility of determining whether NOS neurons have increased or Hu
neurons decreased. Second, I asked whether the increase in NOS expression
was confined to the myenteric plexus. I can't find this information in the revised
version. Third, I requested that they show the size data as a frequency histogram
before and after SAP treatment. This has not been done.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests'
Reviewer's report
Title:Increased proportion of nitric oxide synthase immunoreactive neurons in
rat ileal myenteric ganglia after severe acute pancreatitis
Version: 6Date:20 July 2011
Reviewer: Colin R Anderson
Reviewer's report:
The paper has been largely revised along the lines I requested. However, I
requested three compulsory revisions that have not been made, nor have the
authors responded to the requests. First, I asked whether the Hu neurons
changed in number as this is the only way that the reader can know whether
NOS neurons have increased in number versus whether Hu neurons have
decreased. This has not been answered, but I note that the authors now say they
have counted only 200 Hu neurons at a time, so this implies they don't have the
data to answer my original request. Therefore, I would simply ask that they point
out the impossibility of determining whether NOS neurons have increased or Hu
neurons decreased. Second, I asked whether the increase in NOS expression
was confined to the myenteric plexus. I can't find this information in the revised
version. Third, I requested that they show the size data as a frequency histogram
before and after SAP treatment. This has not been done.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests'
Please review this paper.
user
Reviewer's report
Title:Increased proportion of nitric oxide synthase immunoreactive neurons in
rat ileal myenteric ganglia after severe acute pancreatitis
Version: 6Date:20 July 2011
Reviewer: Colin R Anderson
Reviewer's report:
The paper has been largely revised along the lines I requested. However, I
requested three compulsory revisions that have not been made, nor have the
authors responded to the requests. First, I asked whether the Hu neurons
changed in number as this is the only way that the reader can know whether
NOS neurons have increased in number versus whether Hu neurons have
decreased. This has not been answered, but I note that the authors now say they
have counted only 200 Hu neurons at a time, so this implies they don't have the
data to answer my original request. Therefore, I would simply ask that they point
out the impossibility of determining whether NOS neurons have increased or Hu
neurons decreased. Second, I asked whether the increase in NOS expression
was confined to the myenteric plexus. I can't find this information in the revised
version. Third, I requested that they show the size data as a frequency histogram
before and after SAP treatment. This has not been done.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests'
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