Title: Single-cell transcriptomic analysis reveals prognosis-related stromal signatures that
potentiate stratification of patients with extrahepatic cholangiocarcinoma
Version: 2 Date: 21 Feb 2025
Reviewer:  Hong-Yue Lai
Reviewer's report:
Most issues have been addressed; however, some aspects still require improvement.
1. This study utilizes public-domain datasets that include highly diverse clinicopathological
features and treatment strategies, making it unlikely that all patients were treated under a
consistent protocol. Consequently, the survival impacts observed may lack reliability.
2. Given that confounders such as clinicopathological characteristics, treatment strategies, and
comorbidities may impact patient survival, the authors are encouraged to clarify the independent
role of stromal subpopulations in survival outcomes.
3. These essential data should be included in the manuscript rather than the supplementary
materials. If the data are unavailable, the authors should acknowledge this as a limitation in the
discussion.
Title: Single-cell transcriptomic analysis reveals prognosis-related stromal signatures that
potentiate stratification of patients with extrahepatic cholangiocarcinoma
Version: 2 Date: 21 Feb 2025
Reviewer:  Hong-Yue Lai
Reviewer's report:
Most issues have been addressed; however, some aspects still require improvement.
1. This study utilizes public-domain datasets that include highly diverse clinicopathological
features and treatment strategies, making it unlikely that all patients were treated under a
consistent protocol. Consequently, the survival impacts observed may lack reliability.
2. Given that confounders such as clinicopathological characteristics, treatment strategies, and
comorbidities may impact patient survival, the authors are encouraged to clarify the independent
role of stromal subpopulations in survival outcomes.
3. These essential data should be included in the manuscript rather than the supplementary
materials. If the data are unavailable, the authors should acknowledge this as a limitation in the
discussion.
Please review this paper.
user
Title: Single-cell transcriptomic analysis reveals prognosis-related stromal signatures that
potentiate stratification of patients with extrahepatic cholangiocarcinoma
Version: 2 Date: 21 Feb 2025
Reviewer:  Hong-Yue Lai
Reviewer's report:
Most issues have been addressed; however, some aspects still require improvement.
1. This study utilizes public-domain datasets that include highly diverse clinicopathological
features and treatment strategies, making it unlikely that all patients were treated under a
consistent protocol. Consequently, the survival impacts observed may lack reliability.
2. Given that confounders such as clinicopathological characteristics, treatment strategies, and
comorbidities may impact patient survival, the authors are encouraged to clarify the independent
role of stromal subpopulations in survival outcomes.
3. These essential data should be included in the manuscript rather than the supplementary
materials. If the data are unavailable, the authors should acknowledge this as a limitation in the
discussion.
assistant