Title: A new model of portal vein thrombosis in rats with cirrhosis induced by partial portal
vein ligation plus carbon tetrachloride and intervened with rivaroxaban
Version: 3 Date: 03 Apr 2024
Reviewer's report:
Comments to the authors
Abstract
The authors said “49 male SD rats received partial portal vein ligation (PPVL), and 41 survival
rats were divided into 6 groups: PPVL control group; week 4, 6, 8, and 10 model group”. After
the PPVL operation, 44 rats were divided into 6 groups, not 41. Additionally, it is recommended
that the authors include a chart in the article to more clearly illustrate the group distribution of
the model rats as well as the numbers of deaths and survivals.
Materials and methods
The authors said “We took 49 rats to perform PPVL and intraperitoneal injection of CCl4
to establish a rat model of cirrhosis complicated with PVT”. Not all 49 rats received an
intraperitoneal injection of CCl4; the author should clearly describe this point.
The authors said “The rats after PPVL one week later were divided into 6 groups”. Grouping
rats after the PPVL operation could lead to selection bias: the condition of the rats at the time
of grouping might influence which group they are assigned to. For example, rats that are in
better health or have a stronger recovery ability after the PPVL operation are more likely to be
included in the intervention group. This selection bias could affect the study outcomes, including
the observed survival rates and the efficacy of rivaroxaban intervention. The authors need to
explain this problem.
Results
The authors said “Additionally, the expression of fibrinogen and PAI-1 in rivaroxaban-
treated rats was lower than that in rats in the PPVL+CCl4 10w group, as demonstrated by
immunohistochemical staining and western blotting”. It is mentioned here that fibrinogen was
measured, which conflicts with the results in Figure 7C; the authors need to correct this error.
Title: A new model of portal vein thrombosis in rats with cirrhosis induced by partial portal
vein ligation plus carbon tetrachloride and intervened with rivaroxaban
Version: 3 Date: 03 Apr 2024
Reviewer's report:
Comments to the authors
Abstract
The authors said “49 male SD rats received partial portal vein ligation (PPVL), and 41 survival
rats were divided into 6 groups: PPVL control group; week 4, 6, 8, and 10 model group”. After
the PPVL operation, 44 rats were divided into 6 groups, not 41. Additionally, it is recommended
that the authors include a chart in the article to more clearly illustrate the group distribution of
the model rats as well as the numbers of deaths and survivals.
Materials and methods
The authors said “We took 49 rats to perform PPVL and intraperitoneal injection of CCl4
to establish a rat model of cirrhosis complicated with PVT”. Not all 49 rats received an
intraperitoneal injection of CCl4; the author should clearly describe this point.
The authors said “The rats after PPVL one week later were divided into 6 groups”. Grouping
rats after the PPVL operation could lead to selection bias: the condition of the rats at the time
of grouping might influence which group they are assigned to. For example, rats that are in
better health or have a stronger recovery ability after the PPVL operation are more likely to be
included in the intervention group. This selection bias could affect the study outcomes, including
the observed survival rates and the efficacy of rivaroxaban intervention. The authors need to
explain this problem.
Results
The authors said “Additionally, the expression of fibrinogen and PAI-1 in rivaroxaban-
treated rats was lower than that in rats in the PPVL+CCl4 10w group, as demonstrated by
immunohistochemical staining and western blotting”. It is mentioned here that fibrinogen was
measured, which conflicts with the results in Figure 7C; the authors need to correct this error.
Please review this paper.
user
Title: A new model of portal vein thrombosis in rats with cirrhosis induced by partial portal
vein ligation plus carbon tetrachloride and intervened with rivaroxaban
Version: 3 Date: 03 Apr 2024
Reviewer's report:
Comments to the authors
Abstract
The authors said “49 male SD rats received partial portal vein ligation (PPVL), and 41 survival
rats were divided into 6 groups: PPVL control group; week 4, 6, 8, and 10 model group”. After
the PPVL operation, 44 rats were divided into 6 groups, not 41. Additionally, it is recommended
that the authors include a chart in the article to more clearly illustrate the group distribution of
the model rats as well as the numbers of deaths and survivals.
Materials and methods
The authors said “We took 49 rats to perform PPVL and intraperitoneal injection of CCl4
to establish a rat model of cirrhosis complicated with PVT”. Not all 49 rats received an
intraperitoneal injection of CCl4; the author should clearly describe this point.
The authors said “The rats after PPVL one week later were divided into 6 groups”. Grouping
rats after the PPVL operation could lead to selection bias: the condition of the rats at the time
of grouping might influence which group they are assigned to. For example, rats that are in
better health or have a stronger recovery ability after the PPVL operation are more likely to be
included in the intervention group. This selection bias could affect the study outcomes, including
the observed survival rates and the efficacy of rivaroxaban intervention. The authors need to
explain this problem.
Results
The authors said “Additionally, the expression of fibrinogen and PAI-1 in rivaroxaban-
treated rats was lower than that in rats in the PPVL+CCl4 10w group, as demonstrated by
immunohistochemical staining and western blotting”. It is mentioned here that fibrinogen was
measured, which conflicts with the results in Figure 7C; the authors need to correct this error.
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