Title: The role of m6A regulators mRNA expression in peripheral blood mononuclear cells in
chronic hepatitis B: A novel Diagnostic and prognostic indicator
Version: 1 Date: 08 Dec 2024
Reviewer's report:
The authors have conducted an important study on chronic hepatitis B (CHB), which provides
valuable insights into the role of m6A regulators in disease progression. However, there are
several key points that need to be addressed to strengthen the manuscript.
#The finding that METTL3 expression is correlated with inflammatory markers such as the
neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and lymphocyte-
to-monocyte ratio (LMR) is noteworthy and may have clinical significance. However, the
biological mechanisms linking METTL3 to these inflammatory markers are not fully explained
in the paper. As the role of METTL3 in regulating immune responses is a central hypothesis, a
more detailed discussion of its mechanistic role is needed.
#The use of logistic regression analysis with SPSS 29 is appropriate, and a P-value threshold
of less than 0.05 is acceptable. However, because multiple comparisons are being made, it is
essential to apply appropriate multiple comparison corrections, such as the Bonferroni correction
or false discovery rate (FDR) adjustment.
#The total sample size is relatively small, especially in the subgroup analysis of patients with
liver cirrhosis (LC). The limited number of patients with liver cirrhosis (n=26) may affect
the statistical validity and generalizability of the results. A larger and more diverse cohort is
recommended to increase the reliability of the findings. If it is not possible to increase the
sample size, the authors should discuss the limitations posed by the small sample size in the
"Limitations" section of the paper.
Title: The role of m6A regulators mRNA expression in peripheral blood mononuclear cells in
chronic hepatitis B: A novel Diagnostic and prognostic indicator
Version: 1 Date: 08 Dec 2024
Reviewer's report:
The authors have conducted an important study on chronic hepatitis B (CHB), which provides
valuable insights into the role of m6A regulators in disease progression. However, there are
several key points that need to be addressed to strengthen the manuscript.
#The finding that METTL3 expression is correlated with inflammatory markers such as the
neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and lymphocyte-
to-monocyte ratio (LMR) is noteworthy and may have clinical significance. However, the
biological mechanisms linking METTL3 to these inflammatory markers are not fully explained
in the paper. As the role of METTL3 in regulating immune responses is a central hypothesis, a
more detailed discussion of its mechanistic role is needed.
#The use of logistic regression analysis with SPSS 29 is appropriate, and a P-value threshold
of less than 0.05 is acceptable. However, because multiple comparisons are being made, it is
essential to apply appropriate multiple comparison corrections, such as the Bonferroni correction
or false discovery rate (FDR) adjustment.
#The total sample size is relatively small, especially in the subgroup analysis of patients with
liver cirrhosis (LC). The limited number of patients with liver cirrhosis (n=26) may affect
the statistical validity and generalizability of the results. A larger and more diverse cohort is
recommended to increase the reliability of the findings. If it is not possible to increase the
sample size, the authors should discuss the limitations posed by the small sample size in the
"Limitations" section of the paper.
Please review this paper.
user
Title: The role of m6A regulators mRNA expression in peripheral blood mononuclear cells in
chronic hepatitis B: A novel Diagnostic and prognostic indicator
Version: 1 Date: 08 Dec 2024
Reviewer's report:
The authors have conducted an important study on chronic hepatitis B (CHB), which provides
valuable insights into the role of m6A regulators in disease progression. However, there are
several key points that need to be addressed to strengthen the manuscript.
#The finding that METTL3 expression is correlated with inflammatory markers such as the
neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and lymphocyte-
to-monocyte ratio (LMR) is noteworthy and may have clinical significance. However, the
biological mechanisms linking METTL3 to these inflammatory markers are not fully explained
in the paper. As the role of METTL3 in regulating immune responses is a central hypothesis, a
more detailed discussion of its mechanistic role is needed.
#The use of logistic regression analysis with SPSS 29 is appropriate, and a P-value threshold
of less than 0.05 is acceptable. However, because multiple comparisons are being made, it is
essential to apply appropriate multiple comparison corrections, such as the Bonferroni correction
or false discovery rate (FDR) adjustment.
#The total sample size is relatively small, especially in the subgroup analysis of patients with
liver cirrhosis (LC). The limited number of patients with liver cirrhosis (n=26) may affect
the statistical validity and generalizability of the results. A larger and more diverse cohort is
recommended to increase the reliability of the findings. If it is not possible to increase the
sample size, the authors should discuss the limitations posed by the small sample size in the
"Limitations" section of the paper.
assistant