Reviewer's report
Title:The role of cytoplasmic p57 in invasion of hepatocellular carcinoma
Version:2Date:16 April 2015
Reviewer: Robert Eferl
Reviewer's report:
Major Compulsory Revisions
1. Nuclear staining of p57 is not evident in Figure 1A. The authors should label
nuclei that are considered to be p57-positive with arrows. There seem to be no
cofilin-positive cells in Figure 1C. Have the brownish vesicles been considered as
cofilin-positive signals? Cofilin IHC-staining of cancer tissues including
hepatocellular carcinoma looks different. The reliability of the correlations
between nuclear p57 with tumor size, cytoplasmic p57 with metastasis as well as
cytoplasmic absence of p57/cofilin with TNM stage and metastasis require
improved stainings which should be provided in Figure 1.
2. The authors claim that p57 interacts with LIMK1 and provide
co-immunoprecipitation data in Figure 2D. There is, however, no visible effect on
“co-immunoprecipitation” of LIMK1 in the hepatoma cell lines after knock-down of
p57. The authors should evaluate other mechanisms of p57-mediated regulation
of cofilin expression. Realtime PCR analysis for cofilin mRNA should be
performed to reveal transcriptional versus post-transcriptional mechanisms.
Level of interest: An article of limited interest
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I have no competing interests.
Reviewer's report
Title:The role of cytoplasmic p57 in invasion of hepatocellular carcinoma
Version:2Date:16 April 2015
Reviewer: Robert Eferl
Reviewer's report:
Major Compulsory Revisions
1. Nuclear staining of p57 is not evident in Figure 1A. The authors should label
nuclei that are considered to be p57-positive with arrows. There seem to be no
cofilin-positive cells in Figure 1C. Have the brownish vesicles been considered as
cofilin-positive signals? Cofilin IHC-staining of cancer tissues including
hepatocellular carcinoma looks different. The reliability of the correlations
between nuclear p57 with tumor size, cytoplasmic p57 with metastasis as well as
cytoplasmic absence of p57/cofilin with TNM stage and metastasis require
improved stainings which should be provided in Figure 1.
2. The authors claim that p57 interacts with LIMK1 and provide
co-immunoprecipitation data in Figure 2D. There is, however, no visible effect on
“co-immunoprecipitation” of LIMK1 in the hepatoma cell lines after knock-down of
p57. The authors should evaluate other mechanisms of p57-mediated regulation
of cofilin expression. Realtime PCR analysis for cofilin mRNA should be
performed to reveal transcriptional versus post-transcriptional mechanisms.
Level of interest: An article of limited interest
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:The role of cytoplasmic p57 in invasion of hepatocellular carcinoma
Version:2Date:16 April 2015
Reviewer: Robert Eferl
Reviewer's report:
Major Compulsory Revisions
1. Nuclear staining of p57 is not evident in Figure 1A. The authors should label
nuclei that are considered to be p57-positive with arrows. There seem to be no
cofilin-positive cells in Figure 1C. Have the brownish vesicles been considered as
cofilin-positive signals? Cofilin IHC-staining of cancer tissues including
hepatocellular carcinoma looks different. The reliability of the correlations
between nuclear p57 with tumor size, cytoplasmic p57 with metastasis as well as
cytoplasmic absence of p57/cofilin with TNM stage and metastasis require
improved stainings which should be provided in Figure 1.
2. The authors claim that p57 interacts with LIMK1 and provide
co-immunoprecipitation data in Figure 2D. There is, however, no visible effect on
“co-immunoprecipitation” of LIMK1 in the hepatoma cell lines after knock-down of
p57. The authors should evaluate other mechanisms of p57-mediated regulation
of cofilin expression. Realtime PCR analysis for cofilin mRNA should be
performed to reveal transcriptional versus post-transcriptional mechanisms.
Level of interest: An article of limited interest
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I have no competing interests.
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