Title: Finding key genes (UBE2T, KIF4A, CDCA3, and CDCA5) co-expressed in hepatitis,
cirrhosis and hepatocellular carcinoma based on multiple bioinformatics techniques
Version: 1 Date: 24 Mar 2024
Reviewer:  Kazunori Kawaguchi
Reviewer's report:
Authors analyzed gene expression using various state of HBV infected patients including
HCC from database. Though they found 9 genes were associated with chronic liver injury
development such as CAH, LC, HCC regarding HBV infection there is no novelty since these
molecules were already reported and there is no further analysis for these 9 molecules.
Major points:
1. Though they listed 9 genes for HBV related chronic liver injury development and poor
prognosis, these genes have already been reported in HCC. The reason of lack of originality
arises they and other researchers used same databases, that leads to pick up same candidate
genes listed. By analyzing identical databases without using original clinical samples, it leads
to obtain similar results.
2. It is very unclear about the status of background of the liver in HCC cases by reading
manuscript. There is little information of clinical samples data. There is no information about
HBV infection status such as HBe, HBV-DNA, HBV genotype, fibrosis or chronic liver
injury state, and another fundamental clinical information including HCC staging, pathological
information, and background of patients. This information should be listed clearly in the table.
3. In the discussion they did not explain how 9 genes were important for HBV infected chronic
liver injury.
Minor points:
1. This manuscript looks like unclear since there is no explanation of what kind of genes
candidate. They just expressed ‘key genes’ in the title and abstract, no explanation what kind of
function for HBV related chronic liver injury.
2. In the result for the explanation Figure 4F, 9 genes were identified, in the manuscript. In this
portion authors should explain what kind of genes found.
Title: Finding key genes (UBE2T, KIF4A, CDCA3, and CDCA5) co-expressed in hepatitis,
cirrhosis and hepatocellular carcinoma based on multiple bioinformatics techniques
Version: 1 Date: 24 Mar 2024
Reviewer:  Kazunori Kawaguchi
Reviewer's report:
Authors analyzed gene expression using various state of HBV infected patients including
HCC from database. Though they found 9 genes were associated with chronic liver injury
development such as CAH, LC, HCC regarding HBV infection there is no novelty since these
molecules were already reported and there is no further analysis for these 9 molecules.
Major points:
1. Though they listed 9 genes for HBV related chronic liver injury development and poor
prognosis, these genes have already been reported in HCC. The reason of lack of originality
arises they and other researchers used same databases, that leads to pick up same candidate
genes listed. By analyzing identical databases without using original clinical samples, it leads
to obtain similar results.
2. It is very unclear about the status of background of the liver in HCC cases by reading
manuscript. There is little information of clinical samples data. There is no information about
HBV infection status such as HBe, HBV-DNA, HBV genotype, fibrosis or chronic liver
injury state, and another fundamental clinical information including HCC staging, pathological
information, and background of patients. This information should be listed clearly in the table.
3. In the discussion they did not explain how 9 genes were important for HBV infected chronic
liver injury.
Minor points:
1. This manuscript looks like unclear since there is no explanation of what kind of genes
candidate. They just expressed ‘key genes’ in the title and abstract, no explanation what kind of
function for HBV related chronic liver injury.
2. In the result for the explanation Figure 4F, 9 genes were identified, in the manuscript. In this
portion authors should explain what kind of genes found.
Please review this paper.
user
Title: Finding key genes (UBE2T, KIF4A, CDCA3, and CDCA5) co-expressed in hepatitis,
cirrhosis and hepatocellular carcinoma based on multiple bioinformatics techniques
Version: 1 Date: 24 Mar 2024
Reviewer:  Kazunori Kawaguchi
Reviewer's report:
Authors analyzed gene expression using various state of HBV infected patients including
HCC from database. Though they found 9 genes were associated with chronic liver injury
development such as CAH, LC, HCC regarding HBV infection there is no novelty since these
molecules were already reported and there is no further analysis for these 9 molecules.
Major points:
1. Though they listed 9 genes for HBV related chronic liver injury development and poor
prognosis, these genes have already been reported in HCC. The reason of lack of originality
arises they and other researchers used same databases, that leads to pick up same candidate
genes listed. By analyzing identical databases without using original clinical samples, it leads
to obtain similar results.
2. It is very unclear about the status of background of the liver in HCC cases by reading
manuscript. There is little information of clinical samples data. There is no information about
HBV infection status such as HBe, HBV-DNA, HBV genotype, fibrosis or chronic liver
injury state, and another fundamental clinical information including HCC staging, pathological
information, and background of patients. This information should be listed clearly in the table.
3. In the discussion they did not explain how 9 genes were important for HBV infected chronic
liver injury.
Minor points:
1. This manuscript looks like unclear since there is no explanation of what kind of genes
candidate. They just expressed ‘key genes’ in the title and abstract, no explanation what kind of
function for HBV related chronic liver injury.
2. In the result for the explanation Figure 4F, 9 genes were identified, in the manuscript. In this
portion authors should explain what kind of genes found.
assistant