Title: Identification of SCN7A as the key gene associated with tumor mutation burden in gastric
cancer
Version: 1 Date: 14 Oct 2021
Reviewer's report:
In this manuscript, to identify tumor mutation burden (TMB) related genes in gastric cancer
and to explore their prognostic value, the authors analyzed the gastric cancer data in TCGA
database by different tools. The authors screened the differentially expressed genes, found the
most relevant TMB modules and then identified hub genes. Next, the authors analyzed the
relationship among hub genes and immune cells. Finally, the authors identified gene SCN7A
affected the signaling pathways of immune system and revealed the possibility that SCN7A is
a novel biomarker. In general, this study is a routine work. In addition, there are some issues
should be revised.
1. The expression of abbreviations are not properly. Some abbreviations are not attached the full
name when they first appeared such as TMB and GC in “abstract” section, GS in main body.
And the abbreviation of “TNM” is not attached the full name.
2. The authors cited “figure S2 A-D” in the manuscript, however, there is not a figure S2 in
manuscript or related files. And the authors provided a table file, however there is not citation
in the main body. The authors should confirm and revise them.
3. The format of some references are not consistent with others. The authors should revise them
in consistent format.
4. The identification of hub gene in “Functional enrichment analysis and hub gene identification”
section should be described with more details.
Title: Identification of SCN7A as the key gene associated with tumor mutation burden in gastric
cancer
Version: 1 Date: 14 Oct 2021
Reviewer's report:
In this manuscript, to identify tumor mutation burden (TMB) related genes in gastric cancer
and to explore their prognostic value, the authors analyzed the gastric cancer data in TCGA
database by different tools. The authors screened the differentially expressed genes, found the
most relevant TMB modules and then identified hub genes. Next, the authors analyzed the
relationship among hub genes and immune cells. Finally, the authors identified gene SCN7A
affected the signaling pathways of immune system and revealed the possibility that SCN7A is
a novel biomarker. In general, this study is a routine work. In addition, there are some issues
should be revised.
1. The expression of abbreviations are not properly. Some abbreviations are not attached the full
name when they first appeared such as TMB and GC in “abstract” section, GS in main body.
And the abbreviation of “TNM” is not attached the full name.
2. The authors cited “figure S2 A-D” in the manuscript, however, there is not a figure S2 in
manuscript or related files. And the authors provided a table file, however there is not citation
in the main body. The authors should confirm and revise them.
3. The format of some references are not consistent with others. The authors should revise them
in consistent format.
4. The identification of hub gene in “Functional enrichment analysis and hub gene identification”
section should be described with more details.
Please review this paper.
user
Title: Identification of SCN7A as the key gene associated with tumor mutation burden in gastric
cancer
Version: 1 Date: 14 Oct 2021
Reviewer's report:
In this manuscript, to identify tumor mutation burden (TMB) related genes in gastric cancer
and to explore their prognostic value, the authors analyzed the gastric cancer data in TCGA
database by different tools. The authors screened the differentially expressed genes, found the
most relevant TMB modules and then identified hub genes. Next, the authors analyzed the
relationship among hub genes and immune cells. Finally, the authors identified gene SCN7A
affected the signaling pathways of immune system and revealed the possibility that SCN7A is
a novel biomarker. In general, this study is a routine work. In addition, there are some issues
should be revised.
1. The expression of abbreviations are not properly. Some abbreviations are not attached the full
name when they first appeared such as TMB and GC in “abstract” section, GS in main body.
And the abbreviation of “TNM” is not attached the full name.
2. The authors cited “figure S2 A-D” in the manuscript, however, there is not a figure S2 in
manuscript or related files. And the authors provided a table file, however there is not citation
in the main body. The authors should confirm and revise them.
3. The format of some references are not consistent with others. The authors should revise them
in consistent format.
4. The identification of hub gene in “Functional enrichment analysis and hub gene identification”
section should be described with more details.
assistant