Title: Lipopolysaccharide-Binding Protein (LBP): A Prognostic Biomarker for Gastric Cancer
Linked to Immune Infiltration
Version: 3 Date: 05 Jan 2025
Reviewer:  Anwar Ashraf Abouelnasr
Reviewer's report:
Dear Authors, please address the following points below
-While the abstract mentions LBP's involvement in various cancers (lung, colon, and cervical),
a more detailed discussion of LBP’s role in other cancers would strengthen the case for its study
in GC. Specifically, a short mention of what is known about LBP’s mechanistic contributions
to other cancers could provide a better context.
-The introduction alludes to LBP's potential role in GC pathogenesis but could clarify whether
the research aims to establish causal or merely associative relationships between LBP and GC.
This would refine the hypothesis and make the study's objectives clearer to the reader.
-While the RNA sequencing data acquisition is well-described, more information on data quality
control would be beneficial. Specifically, how were low-quality samples or outliers handled
before analysis? Providing this detail would increase the transparency of the data processing
pipeline.
-While the cell lines used are mentioned, it would be helpful to provide more information about
their relevance to GC. Are these cell lines representative of different subtypes of gastric cancer?
A brief description of their characteristics would add context to the experimental design.
-While the manuscript suggests several mechanisms through which LBP contributes to gastric
cancer, the mechanistic details could be more fleshed out. Specifically, the signaling pathways
such as NF-#B and STAT3 are mentioned, but a more detailed exploration of how LBP
modulates these pathways in the context of gastric cancer is warranted.
-The paper briefly touches on immune evasion, particularly regarding Tregs and TAMs, but
more in-depth discussion on the molecular interactions between LBP and immune cells would
strengthen the narrative. How exactly does LBP recruit or influence the polarization of these
immune cells? Are there any direct interactions between LBP and immune checkpoints?
Title: Lipopolysaccharide-Binding Protein (LBP): A Prognostic Biomarker for Gastric Cancer
Linked to Immune Infiltration
Version: 3 Date: 05 Jan 2025
Reviewer:  Anwar Ashraf Abouelnasr
Reviewer's report:
Dear Authors, please address the following points below
-While the abstract mentions LBP's involvement in various cancers (lung, colon, and cervical),
a more detailed discussion of LBP’s role in other cancers would strengthen the case for its study
in GC. Specifically, a short mention of what is known about LBP’s mechanistic contributions
to other cancers could provide a better context.
-The introduction alludes to LBP's potential role in GC pathogenesis but could clarify whether
the research aims to establish causal or merely associative relationships between LBP and GC.
This would refine the hypothesis and make the study's objectives clearer to the reader.
-While the RNA sequencing data acquisition is well-described, more information on data quality
control would be beneficial. Specifically, how were low-quality samples or outliers handled
before analysis? Providing this detail would increase the transparency of the data processing
pipeline.
-While the cell lines used are mentioned, it would be helpful to provide more information about
their relevance to GC. Are these cell lines representative of different subtypes of gastric cancer?
A brief description of their characteristics would add context to the experimental design.
-While the manuscript suggests several mechanisms through which LBP contributes to gastric
cancer, the mechanistic details could be more fleshed out. Specifically, the signaling pathways
such as NF-#B and STAT3 are mentioned, but a more detailed exploration of how LBP
modulates these pathways in the context of gastric cancer is warranted.
-The paper briefly touches on immune evasion, particularly regarding Tregs and TAMs, but
more in-depth discussion on the molecular interactions between LBP and immune cells would
strengthen the narrative. How exactly does LBP recruit or influence the polarization of these
immune cells? Are there any direct interactions between LBP and immune checkpoints?
Please review this paper.
user
Title: Lipopolysaccharide-Binding Protein (LBP): A Prognostic Biomarker for Gastric Cancer
Linked to Immune Infiltration
Version: 3 Date: 05 Jan 2025
Reviewer:  Anwar Ashraf Abouelnasr
Reviewer's report:
Dear Authors, please address the following points below
-While the abstract mentions LBP's involvement in various cancers (lung, colon, and cervical),
a more detailed discussion of LBP’s role in other cancers would strengthen the case for its study
in GC. Specifically, a short mention of what is known about LBP’s mechanistic contributions
to other cancers could provide a better context.
-The introduction alludes to LBP's potential role in GC pathogenesis but could clarify whether
the research aims to establish causal or merely associative relationships between LBP and GC.
This would refine the hypothesis and make the study's objectives clearer to the reader.
-While the RNA sequencing data acquisition is well-described, more information on data quality
control would be beneficial. Specifically, how were low-quality samples or outliers handled
before analysis? Providing this detail would increase the transparency of the data processing
pipeline.
-While the cell lines used are mentioned, it would be helpful to provide more information about
their relevance to GC. Are these cell lines representative of different subtypes of gastric cancer?
A brief description of their characteristics would add context to the experimental design.
-While the manuscript suggests several mechanisms through which LBP contributes to gastric
cancer, the mechanistic details could be more fleshed out. Specifically, the signaling pathways
such as NF-#B and STAT3 are mentioned, but a more detailed exploration of how LBP
modulates these pathways in the context of gastric cancer is warranted.
-The paper briefly touches on immune evasion, particularly regarding Tregs and TAMs, but
more in-depth discussion on the molecular interactions between LBP and immune cells would
strengthen the narrative. How exactly does LBP recruit or influence the polarization of these
immune cells? Are there any direct interactions between LBP and immune checkpoints?
assistant