Reviewer's report
Title:Transcript levels of Toll-like receptors 5, 8 and 9 correlate with
inflammatory activity in Ulcerative Colitis
Version: 3Date:22 January 2011
Reviewer: Mathias Chamaillard
Reviewer's report:
The manuscript by Sánchez-Muñoz et al showed a significant correlation
between disease activity and colonic expression of TLR5, TLR8 and TLR9
mRNA levels in Ulcerative colitis. The findings are consistent with previous
reports showing an increased expression of the latter pattern recognition
receptors in active disease (Int J Colorectal Dis. 2007 Mar;22(3):293-301; Scand
J Gastroenterol. 2009;44(2):195-204; Inflamm Bowel Dis. 2010
Aug;16(8):1267-8). The manuscript is well written and easy to follow. However, it
lacks an in-depth functional analysis to assess the relevance of the expression
changes of TLR5, TLR8 and TLR9 on disease pathogenesis. Whereas the
methodology used in this study is adequate, here are some suggestions that, I
think, need to be considered :
- Is there any abnormal responsiveness to TLR5, TLR8 and TLR9 agonists of
colonocytes from UC patients with quiescent disease?
- Is there any influence of the therapeutical management of the UC patients on
changes of TLR5, TLR8 and TLR9 transcript levels?
Specific comments:
- In the abstract, the authors wrote that 50 UC patients were included in their
study while in the methods section they mentioned that rectum biospies were
collected from 60 UC patients. This is confusing.
Level of interest: An article of insufficient interest to warrant publication in a
scientific/medical journal
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Transcript levels of Toll-like receptors 5, 8 and 9 correlate with
inflammatory activity in Ulcerative Colitis
Version: 3Date:22 January 2011
Reviewer: Mathias Chamaillard
Reviewer's report:
The manuscript by Sánchez-Muñoz et al showed a significant correlation
between disease activity and colonic expression of TLR5, TLR8 and TLR9
mRNA levels in Ulcerative colitis. The findings are consistent with previous
reports showing an increased expression of the latter pattern recognition
receptors in active disease (Int J Colorectal Dis. 2007 Mar;22(3):293-301; Scand
J Gastroenterol. 2009;44(2):195-204; Inflamm Bowel Dis. 2010
Aug;16(8):1267-8). The manuscript is well written and easy to follow. However, it
lacks an in-depth functional analysis to assess the relevance of the expression
changes of TLR5, TLR8 and TLR9 on disease pathogenesis. Whereas the
methodology used in this study is adequate, here are some suggestions that, I
think, need to be considered :
- Is there any abnormal responsiveness to TLR5, TLR8 and TLR9 agonists of
colonocytes from UC patients with quiescent disease?
- Is there any influence of the therapeutical management of the UC patients on
changes of TLR5, TLR8 and TLR9 transcript levels?
Specific comments:
- In the abstract, the authors wrote that 50 UC patients were included in their
study while in the methods section they mentioned that rectum biospies were
collected from 60 UC patients. This is confusing.
Level of interest: An article of insufficient interest to warrant publication in a
scientific/medical journal
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Transcript levels of Toll-like receptors 5, 8 and 9 correlate with
inflammatory activity in Ulcerative Colitis
Version: 3Date:22 January 2011
Reviewer: Mathias Chamaillard
Reviewer's report:
The manuscript by Sánchez-Muñoz et al showed a significant correlation
between disease activity and colonic expression of TLR5, TLR8 and TLR9
mRNA levels in Ulcerative colitis. The findings are consistent with previous
reports showing an increased expression of the latter pattern recognition
receptors in active disease (Int J Colorectal Dis. 2007 Mar;22(3):293-301; Scand
J Gastroenterol. 2009;44(2):195-204; Inflamm Bowel Dis. 2010
Aug;16(8):1267-8). The manuscript is well written and easy to follow. However, it
lacks an in-depth functional analysis to assess the relevance of the expression
changes of TLR5, TLR8 and TLR9 on disease pathogenesis. Whereas the
methodology used in this study is adequate, here are some suggestions that, I
think, need to be considered :
- Is there any abnormal responsiveness to TLR5, TLR8 and TLR9 agonists of
colonocytes from UC patients with quiescent disease?
- Is there any influence of the therapeutical management of the UC patients on
changes of TLR5, TLR8 and TLR9 transcript levels?
Specific comments:
- In the abstract, the authors wrote that 50 UC patients were included in their
study while in the methods section they mentioned that rectum biospies were
collected from 60 UC patients. This is confusing.
Level of interest: An article of insufficient interest to warrant publication in a
scientific/medical journal
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
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