Title: A simplified multiplex methylated DNA testing for early detection of colorectal cancer
in stool DNA
Version: 1 Date: 16 Aug 2022
Reviewer:  Mai-britt Worm Orntoft
Reviewer's report:
Thank you for the revisioned version of the manuscript.
I think your revised manuscript has improved compared to the original version.
Best of luck with you validation of you test in a an actual screening cohort. I predict that the
loss of specificity will prove an obstacle for actual implementation, as your supplemental table
3 clearly showed, that with a control group more similar to the cases, specificity dropped below
90%.  The iFOBT test have a specificity above 95%, as I recall.
A side-by-side comparison of your test and the iFOBT test would be most interesting.
Best regards.
Title: A simplified multiplex methylated DNA testing for early detection of colorectal cancer
in stool DNA
Version: 1 Date: 16 Aug 2022
Reviewer:  Mai-britt Worm Orntoft
Reviewer's report:
Thank you for the revisioned version of the manuscript.
I think your revised manuscript has improved compared to the original version.
Best of luck with you validation of you test in a an actual screening cohort. I predict that the
loss of specificity will prove an obstacle for actual implementation, as your supplemental table
3 clearly showed, that with a control group more similar to the cases, specificity dropped below
90%.  The iFOBT test have a specificity above 95%, as I recall.
A side-by-side comparison of your test and the iFOBT test would be most interesting.
Best regards.
Please review this paper.
user
Title: A simplified multiplex methylated DNA testing for early detection of colorectal cancer
in stool DNA
Version: 1 Date: 16 Aug 2022
Reviewer:  Mai-britt Worm Orntoft
Reviewer's report:
Thank you for the revisioned version of the manuscript.
I think your revised manuscript has improved compared to the original version.
Best of luck with you validation of you test in a an actual screening cohort. I predict that the
loss of specificity will prove an obstacle for actual implementation, as your supplemental table
3 clearly showed, that with a control group more similar to the cases, specificity dropped below
90%.  The iFOBT test have a specificity above 95%, as I recall.
A side-by-side comparison of your test and the iFOBT test would be most interesting.
Best regards.
assistant