Reviewer's report
Title:The Distribution of jhp0940, jhp0945, jhp0947, jhp0949 and jhp0951 genes
of Helicobacter pylori in China
Version:2Date:6 July 2015
Reviewer: Javed Yakoob
Reviewer's report:
In this manuscript, Yanan Gong and coauthors studied the prevalence of the
plasticity region genes of the H. pylori strains in China. The aim of this study was
to examine the status of the of Helicobacter pylori plasticity region genes and
assess the relationship between the genes and the diseases caused by H. pylori
infection. A total of 141 strains were isolated from patients with chronic active
gastritis, peptic ulcer disease and gastric carcinoma. The prevalence of jhp0940,
jhp0945, 53 jhp0947, jhp0949 and jhp0951 was determined using PCR, and the
results were analyzed using the chi-squared test.
Major comments:
1. Lack of comparison between the distribution of the plasticity region genes of
the H. pylori strains in China and its neighboring countries. Sugimoto M, et al. J
Clin Microbiol 2012 Feb; 50(2): 441–448; Kausar F, et al. J Clin Microbiol. 2005
Apr; 43(4): 1538–1545; Yakoob J, et al. Gut Liver. 2010 September; 4(3):
345–350.
2. Sequencing of the genes would have added strength to the study and further
helped in comparison with the local and regional H. pylori strains.
3. Other H. pylori virulence marker such as cagA, vacA alleles were not studied
that are also associated with the H. pylori associated diseases and would have
further contributed to understanding of hyperplastic region genes association with
complications.
Minor Comments
Results
From lines 153 to 158 on page 7 and 8, results can be presented under a
separate subheading of “geographical variation of hyperplastic region genes”.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
'I declare that I have no competing interests'
Reviewer's report
Title:The Distribution of jhp0940, jhp0945, jhp0947, jhp0949 and jhp0951 genes
of Helicobacter pylori in China
Version:2Date:6 July 2015
Reviewer: Javed Yakoob
Reviewer's report:
In this manuscript, Yanan Gong and coauthors studied the prevalence of the
plasticity region genes of the H. pylori strains in China. The aim of this study was
to examine the status of the of Helicobacter pylori plasticity region genes and
assess the relationship between the genes and the diseases caused by H. pylori
infection. A total of 141 strains were isolated from patients with chronic active
gastritis, peptic ulcer disease and gastric carcinoma. The prevalence of jhp0940,
jhp0945, 53 jhp0947, jhp0949 and jhp0951 was determined using PCR, and the
results were analyzed using the chi-squared test.
Major comments:
1. Lack of comparison between the distribution of the plasticity region genes of
the H. pylori strains in China and its neighboring countries. Sugimoto M, et al. J
Clin Microbiol 2012 Feb; 50(2): 441–448; Kausar F, et al. J Clin Microbiol. 2005
Apr; 43(4): 1538–1545; Yakoob J, et al. Gut Liver. 2010 September; 4(3):
345–350.
2. Sequencing of the genes would have added strength to the study and further
helped in comparison with the local and regional H. pylori strains.
3. Other H. pylori virulence marker such as cagA, vacA alleles were not studied
that are also associated with the H. pylori associated diseases and would have
further contributed to understanding of hyperplastic region genes association with
complications.
Minor Comments
Results
From lines 153 to 158 on page 7 and 8, results can be presented under a
separate subheading of “geographical variation of hyperplastic region genes”.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
'I declare that I have no competing interests'
Please review this paper.
user
Reviewer's report
Title:The Distribution of jhp0940, jhp0945, jhp0947, jhp0949 and jhp0951 genes
of Helicobacter pylori in China
Version:2Date:6 July 2015
Reviewer: Javed Yakoob
Reviewer's report:
In this manuscript, Yanan Gong and coauthors studied the prevalence of the
plasticity region genes of the H. pylori strains in China. The aim of this study was
to examine the status of the of Helicobacter pylori plasticity region genes and
assess the relationship between the genes and the diseases caused by H. pylori
infection. A total of 141 strains were isolated from patients with chronic active
gastritis, peptic ulcer disease and gastric carcinoma. The prevalence of jhp0940,
jhp0945, 53 jhp0947, jhp0949 and jhp0951 was determined using PCR, and the
results were analyzed using the chi-squared test.
Major comments:
1. Lack of comparison between the distribution of the plasticity region genes of
the H. pylori strains in China and its neighboring countries. Sugimoto M, et al. J
Clin Microbiol 2012 Feb; 50(2): 441–448; Kausar F, et al. J Clin Microbiol. 2005
Apr; 43(4): 1538–1545; Yakoob J, et al. Gut Liver. 2010 September; 4(3):
345–350.
2. Sequencing of the genes would have added strength to the study and further
helped in comparison with the local and regional H. pylori strains.
3. Other H. pylori virulence marker such as cagA, vacA alleles were not studied
that are also associated with the H. pylori associated diseases and would have
further contributed to understanding of hyperplastic region genes association with
complications.
Minor Comments
Results
From lines 153 to 158 on page 7 and 8, results can be presented under a
separate subheading of “geographical variation of hyperplastic region genes”.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, but I do not feel adequately qualified to assess the
statistics.
Declaration of competing interests:
'I declare that I have no competing interests'
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