Title: Neutrophil extracellular traps predict postoperative pulmonary complications in paediatric
patients undergoing parental liver transplantation
Version: 1 Date: 14 Feb 2023
Reviewer's report:
"PEER REVIEWER ASSESSMENTS:
OBJECTIVE - Full research articles: is there a clear objective that addresses a testable research
question(s) (brief or other article types: is there a clear objective)?
Yes - there is a clear objective
DESIGN - Is the current approach (including controls and analysis protocols) appropriate for
the objective?
Yes - the approach is appropriate
EXECUTION - Are the experiments and analyses performed with technical rigor to allow
confidence in the results?
Yes - experiments and analyses were performed appropriately
STATISTICS - Is the use of statistics in the manuscript appropriate?
Yes - appropriate statistical analyses have been used in the study
INTERPRETATION - Is the current interpretation/discussion of the results reasonable and not
overstated?
Yes - the author's interpretation is reasonable
OVERALL MANUSCRIPT POTENTIAL - Is the current version of this work technically
sound? If not, can revisions be made to make the work technically sound?
Yes - current version is technically sound
PEER REVIEWER COMMENTS:
GENERAL COMMENTS: Authors have evaluated NETs as biomarkers of pulmonary
complications arising out of parental liver transplantation in pediatric patients. Study topic is
interesting and of importance. Authors have recruited sufficient number of patients and have
evaluated NETs by 4 different techniques including immunostaining which provides strength
to the study.
The major findings of the present study are: different components of NETs could serve as a
biomarker for the development of PPC, risk factor of prolonged ICU stay and even prognostic
indicator of mortality at 1 year. Authors have evaluated NETs by four different techniques;
cfDNA, DNA-histone complexes, DNA-MPO complexes, and immunostaining on confocal
microscopy. Authors have recruited quite a good number of patients (n=133) with a complete 1-
year follow-up of all the patients. Study raises serious relationship between NETs and PPC and
can be utilized by other researchers in this field  to further delve into the complex relationship
between NETs and PPC."
Title: Neutrophil extracellular traps predict postoperative pulmonary complications in paediatric
patients undergoing parental liver transplantation
Version: 1 Date: 14 Feb 2023
Reviewer's report:
"PEER REVIEWER ASSESSMENTS:
OBJECTIVE - Full research articles: is there a clear objective that addresses a testable research
question(s) (brief or other article types: is there a clear objective)?
Yes - there is a clear objective
DESIGN - Is the current approach (including controls and analysis protocols) appropriate for
the objective?
Yes - the approach is appropriate
EXECUTION - Are the experiments and analyses performed with technical rigor to allow
confidence in the results?
Yes - experiments and analyses were performed appropriately
STATISTICS - Is the use of statistics in the manuscript appropriate?
Yes - appropriate statistical analyses have been used in the study
INTERPRETATION - Is the current interpretation/discussion of the results reasonable and not
overstated?
Yes - the author's interpretation is reasonable
OVERALL MANUSCRIPT POTENTIAL - Is the current version of this work technically
sound? If not, can revisions be made to make the work technically sound?
Yes - current version is technically sound
PEER REVIEWER COMMENTS:
GENERAL COMMENTS: Authors have evaluated NETs as biomarkers of pulmonary
complications arising out of parental liver transplantation in pediatric patients. Study topic is
interesting and of importance. Authors have recruited sufficient number of patients and have
evaluated NETs by 4 different techniques including immunostaining which provides strength
to the study.
The major findings of the present study are: different components of NETs could serve as a
biomarker for the development of PPC, risk factor of prolonged ICU stay and even prognostic
indicator of mortality at 1 year. Authors have evaluated NETs by four different techniques;
cfDNA, DNA-histone complexes, DNA-MPO complexes, and immunostaining on confocal
microscopy. Authors have recruited quite a good number of patients (n=133) with a complete 1-
year follow-up of all the patients. Study raises serious relationship between NETs and PPC and
can be utilized by other researchers in this field  to further delve into the complex relationship
between NETs and PPC."
Please review this paper.
user
Title: Neutrophil extracellular traps predict postoperative pulmonary complications in paediatric
patients undergoing parental liver transplantation
Version: 1 Date: 14 Feb 2023
Reviewer's report:
"PEER REVIEWER ASSESSMENTS:
OBJECTIVE - Full research articles: is there a clear objective that addresses a testable research
question(s) (brief or other article types: is there a clear objective)?
Yes - there is a clear objective
DESIGN - Is the current approach (including controls and analysis protocols) appropriate for
the objective?
Yes - the approach is appropriate
EXECUTION - Are the experiments and analyses performed with technical rigor to allow
confidence in the results?
Yes - experiments and analyses were performed appropriately
STATISTICS - Is the use of statistics in the manuscript appropriate?
Yes - appropriate statistical analyses have been used in the study
INTERPRETATION - Is the current interpretation/discussion of the results reasonable and not
overstated?
Yes - the author's interpretation is reasonable
OVERALL MANUSCRIPT POTENTIAL - Is the current version of this work technically
sound? If not, can revisions be made to make the work technically sound?
Yes - current version is technically sound
PEER REVIEWER COMMENTS:
GENERAL COMMENTS: Authors have evaluated NETs as biomarkers of pulmonary
complications arising out of parental liver transplantation in pediatric patients. Study topic is
interesting and of importance. Authors have recruited sufficient number of patients and have
evaluated NETs by 4 different techniques including immunostaining which provides strength
to the study.
The major findings of the present study are: different components of NETs could serve as a
biomarker for the development of PPC, risk factor of prolonged ICU stay and even prognostic
indicator of mortality at 1 year. Authors have evaluated NETs by four different techniques;
cfDNA, DNA-histone complexes, DNA-MPO complexes, and immunostaining on confocal
microscopy. Authors have recruited quite a good number of patients (n=133) with a complete 1-
year follow-up of all the patients. Study raises serious relationship between NETs and PPC and
can be utilized by other researchers in this field  to further delve into the complex relationship
between NETs and PPC."
assistant