Reviewer's report
Title:Growth Inhibition and Apoptosis Induced by 6-fluoro-3-formylchromone in
Hepatocellular Carcinoma
Version:1Date:2 January 2014
Reviewer: yi huang
Reviewer's report:
The authors intended to explore the effects of 6-fluoro-3-formylchromone on
hepatocellular carcinoma cell line SMMC-7721 and some interesting results were
revealed. There are a few comments for the authors:
1 In the abstract, the concentrations of 6-fluoro-3-formylchromone and incubation
time for detecting PCNA, Bax and Bcl-2 should be supplemented.
2 In the introduction, the authors mentioned that “FCC is one of the most
cytotoxic 3-formylchromone derivatives against tumor cell lines such as HSC-2,
HSC-3, HL-60 and human submandibular gland carcinoma cell line”, while their
mechanisms were not involved.
3 We could found that different concentrations of FCC (0, 2, 5, 10, and 20 µg/ml)
were incubated with SMMC-7721. It might be reasonable for the authors to
explain the reason why these concentrations were selected.
4 Though this study revealed that 6-fluoro-3-formylchromone could inhibit the
proliferation of SMMC-7721 cells, the effects of 6-fluoro-3-formylchromone on
hepatocellular carcinoma were not investigated in animal models. Therefore, the
further study design needs to be stated in the discussion.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Growth Inhibition and Apoptosis Induced by 6-fluoro-3-formylchromone in
Hepatocellular Carcinoma
Version:1Date:2 January 2014
Reviewer: yi huang
Reviewer's report:
The authors intended to explore the effects of 6-fluoro-3-formylchromone on
hepatocellular carcinoma cell line SMMC-7721 and some interesting results were
revealed. There are a few comments for the authors:
1 In the abstract, the concentrations of 6-fluoro-3-formylchromone and incubation
time for detecting PCNA, Bax and Bcl-2 should be supplemented.
2 In the introduction, the authors mentioned that “FCC is one of the most
cytotoxic 3-formylchromone derivatives against tumor cell lines such as HSC-2,
HSC-3, HL-60 and human submandibular gland carcinoma cell line”, while their
mechanisms were not involved.
3 We could found that different concentrations of FCC (0, 2, 5, 10, and 20 µg/ml)
were incubated with SMMC-7721. It might be reasonable for the authors to
explain the reason why these concentrations were selected.
4 Though this study revealed that 6-fluoro-3-formylchromone could inhibit the
proliferation of SMMC-7721 cells, the effects of 6-fluoro-3-formylchromone on
hepatocellular carcinoma were not investigated in animal models. Therefore, the
further study design needs to be stated in the discussion.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Growth Inhibition and Apoptosis Induced by 6-fluoro-3-formylchromone in
Hepatocellular Carcinoma
Version:1Date:2 January 2014
Reviewer: yi huang
Reviewer's report:
The authors intended to explore the effects of 6-fluoro-3-formylchromone on
hepatocellular carcinoma cell line SMMC-7721 and some interesting results were
revealed. There are a few comments for the authors:
1 In the abstract, the concentrations of 6-fluoro-3-formylchromone and incubation
time for detecting PCNA, Bax and Bcl-2 should be supplemented.
2 In the introduction, the authors mentioned that “FCC is one of the most
cytotoxic 3-formylchromone derivatives against tumor cell lines such as HSC-2,
HSC-3, HL-60 and human submandibular gland carcinoma cell line”, while their
mechanisms were not involved.
3 We could found that different concentrations of FCC (0, 2, 5, 10, and 20 µg/ml)
were incubated with SMMC-7721. It might be reasonable for the authors to
explain the reason why these concentrations were selected.
4 Though this study revealed that 6-fluoro-3-formylchromone could inhibit the
proliferation of SMMC-7721 cells, the effects of 6-fluoro-3-formylchromone on
hepatocellular carcinoma were not investigated in animal models. Therefore, the
further study design needs to be stated in the discussion.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I declare that I have no competing interests
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