Reviewer's report
Title:DNA Methylation Subgroups and the CpG Island Methylator Phenotype in
Gastric Cancer: A Comprehensive Profiling Approach
Version:2Date:8 November 2013
Reviewer: Alfred SL Cheng
Reviewer's report:
Major Compulsory Revisions
1. In the Conclusion of the abstract, the authors stated that 'High-throughput
methylation analysis implicates genes involved in embryonic development and
hedgehog signaling in gastric tumorigenesis.' It is an interesting conclusion yet to
be confirmed. Although the GoldenGate methylation platform is well-established,
potential important biological findings ought to be confirmed by an independent
method. The methylation of 2-3 identified genes in hedgehog signaling is
required to be confirmed.
Minor Essential Revisions
2. If true normal patients are not involved in the analysis, the authors should
change the wording from ‘normal gastric tissue’ to tumor-adjacent gastric tissues.
3. The authors should then state in the Discussion that 'we believe that the
high-quality tissues used in our study allows us to add value to the available
scientific knowledge'.
Level of interest: An article of limited interest
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:DNA Methylation Subgroups and the CpG Island Methylator Phenotype in
Gastric Cancer: A Comprehensive Profiling Approach
Version:2Date:8 November 2013
Reviewer: Alfred SL Cheng
Reviewer's report:
Major Compulsory Revisions
1. In the Conclusion of the abstract, the authors stated that 'High-throughput
methylation analysis implicates genes involved in embryonic development and
hedgehog signaling in gastric tumorigenesis.' It is an interesting conclusion yet to
be confirmed. Although the GoldenGate methylation platform is well-established,
potential important biological findings ought to be confirmed by an independent
method. The methylation of 2-3 identified genes in hedgehog signaling is
required to be confirmed.
Minor Essential Revisions
2. If true normal patients are not involved in the analysis, the authors should
change the wording from ‘normal gastric tissue’ to tumor-adjacent gastric tissues.
3. The authors should then state in the Discussion that 'we believe that the
high-quality tissues used in our study allows us to add value to the available
scientific knowledge'.
Level of interest: An article of limited interest
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:DNA Methylation Subgroups and the CpG Island Methylator Phenotype in
Gastric Cancer: A Comprehensive Profiling Approach
Version:2Date:8 November 2013
Reviewer: Alfred SL Cheng
Reviewer's report:
Major Compulsory Revisions
1. In the Conclusion of the abstract, the authors stated that 'High-throughput
methylation analysis implicates genes involved in embryonic development and
hedgehog signaling in gastric tumorigenesis.' It is an interesting conclusion yet to
be confirmed. Although the GoldenGate methylation platform is well-established,
potential important biological findings ought to be confirmed by an independent
method. The methylation of 2-3 identified genes in hedgehog signaling is
required to be confirmed.
Minor Essential Revisions
2. If true normal patients are not involved in the analysis, the authors should
change the wording from ‘normal gastric tissue’ to tumor-adjacent gastric tissues.
3. The authors should then state in the Discussion that 'we believe that the
high-quality tissues used in our study allows us to add value to the available
scientific knowledge'.
Level of interest: An article of limited interest
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a statistician.
Declaration of competing interests:
I declare that I have no competing interests.
assistant