Title: Triclosan Exposure Induced Disturbance of Gut Microbiota and Exaggerated
Experimental Colitis in Mice
Version: 1 Date: 10 Jun 2022
Reviewer's report:
* Please  describe the methods carefully. It is not clear what method (protein, mRNA) was used
to assess expression of p65, occludin, TLR4 and others. And what housekeeping gene/protein
was used as control. It will be important to show representative protein (western blot) bands.
* Histology methods mentions 'Liver' samples, please read the manuscript carefully. Describe
how histology score in DSS was calculated.
* Include information on how many mice were used per group and if independent experiments
were conducted.
* Please check the panel numbers in the text.
* In fig 7, cytokine levels of controls should be included to compare the changes caused by DSS.
* Do authors have data on body weights in DSS colitis?
* What is "intraepithelial neoplasia" is not clear.
* What does author mean by "non-specific colitis?" (page 11, line 7)
* The conclusions are not fully supported by the data. For instance, bacterial translocation (page
11, line 5) is not actually shown. Please modify the language to match the actual results.
* Since the casual role of TCS-induced alterations in microbiota in promoting colitis is not
shown, I suggest modifying the title (e.g. Triclosan Exposure Induced  Disturbance of Gut
Microbiota and Exaggerated Experimental Colitis in Mice).
Title: Triclosan Exposure Induced Disturbance of Gut Microbiota and Exaggerated
Experimental Colitis in Mice
Version: 1 Date: 10 Jun 2022
Reviewer's report:
* Please  describe the methods carefully. It is not clear what method (protein, mRNA) was used
to assess expression of p65, occludin, TLR4 and others. And what housekeeping gene/protein
was used as control. It will be important to show representative protein (western blot) bands.
* Histology methods mentions 'Liver' samples, please read the manuscript carefully. Describe
how histology score in DSS was calculated.
* Include information on how many mice were used per group and if independent experiments
were conducted.
* Please check the panel numbers in the text.
* In fig 7, cytokine levels of controls should be included to compare the changes caused by DSS.
* Do authors have data on body weights in DSS colitis?
* What is "intraepithelial neoplasia" is not clear.
* What does author mean by "non-specific colitis?" (page 11, line 7)
* The conclusions are not fully supported by the data. For instance, bacterial translocation (page
11, line 5) is not actually shown. Please modify the language to match the actual results.
* Since the casual role of TCS-induced alterations in microbiota in promoting colitis is not
shown, I suggest modifying the title (e.g. Triclosan Exposure Induced  Disturbance of Gut
Microbiota and Exaggerated Experimental Colitis in Mice).
Please review this paper.
user
Title: Triclosan Exposure Induced Disturbance of Gut Microbiota and Exaggerated
Experimental Colitis in Mice
Version: 1 Date: 10 Jun 2022
Reviewer's report:
* Please  describe the methods carefully. It is not clear what method (protein, mRNA) was used
to assess expression of p65, occludin, TLR4 and others. And what housekeeping gene/protein
was used as control. It will be important to show representative protein (western blot) bands.
* Histology methods mentions 'Liver' samples, please read the manuscript carefully. Describe
how histology score in DSS was calculated.
* Include information on how many mice were used per group and if independent experiments
were conducted.
* Please check the panel numbers in the text.
* In fig 7, cytokine levels of controls should be included to compare the changes caused by DSS.
* Do authors have data on body weights in DSS colitis?
* What is "intraepithelial neoplasia" is not clear.
* What does author mean by "non-specific colitis?" (page 11, line 7)
* The conclusions are not fully supported by the data. For instance, bacterial translocation (page
11, line 5) is not actually shown. Please modify the language to match the actual results.
* Since the casual role of TCS-induced alterations in microbiota in promoting colitis is not
shown, I suggest modifying the title (e.g. Triclosan Exposure Induced  Disturbance of Gut
Microbiota and Exaggerated Experimental Colitis in Mice).
assistant