Reviewer’s report  
 
Title: Rapidly declining trend of signet ring cell cancer of the stomach may parallel the 
infection rate of Helicobacter pylori  
 
Version: 1 Date: 07 Sep 2019  
 
Reviewer: Giovanni Mario Pes  
 
Reviewer's report:  
 
The authors have responded exhaustively and satisfactorily to all my concerns, and added 
new sequence data making the results more robust. They also amended the few mistyping 
errors in the text. I have no other comments and I congratulate for their excelle nt work.  
 
Are the methods appropriate and well described?  
If not, please specify what is required in your comments to the authors.  
 
Yes 
 
Does the work include the necessary controls?  
If not, please specif y which controls are required in your comments to the authors.  
 
Yes 
 
Are the conclusions drawn adequately supported by the data shown?  
If not, please explain in your comments to the authors.  
 
Yes 
 
Are you able to assess any statistics in the manuscript or would you recommend an 
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects require further 
assessment in your comments to the editors.  
 
I am able to assess the statistics  
 
Quality of written English  
Please indicate the quality of language in the manuscript:  
 
Acceptable  
 
Declaration of competing interests  
Please complete a declaration of competing interests, considering the following questions:  
 
1. Have you in the past five years received reimburs ements, fees, funding, or salary from 
an organisation that may in any way gain or lose financially from the publication of 
this manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares in an organisation that may in any way gain or lose 
financially from the publication of this manuscript, either now or in the future?  
 
3. Do you hold or are you currently applying for any patents relating to the content of 
the manuscript?  
 
4. Have you received reimbursements, fees, funding, or salary from an organization that 
holds or has applied for patents relating to the content of the manuscript?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have any non -financial competing interests in relation to this paper?  
 
If you can answer no to all o f the above, write 'I declare that I have no competing interests' 
below.  
If your reply is yes to any, please give details below.  
 
I declare that I have no competing interests  
 
I agree to the open peer review policy of the journal. I understand that my name  will be 
included on my report to the authors and, if the manuscript is accepted for publication, my 
named report including any attachments I upload will be posted on the website along with the 
authors' responses. I agree for my report to be made available  under an Open Access Creative 
Commons CC -BY license ( http://creativecommons.org/licenses/by/4.0/ ). I understand that 
any comments which I do not wish to be included in my named report can be incl uded as 
confidential comments to the editors, which will not be published.  
 
I agree to the open peer review policy of the journal  
 
 
Reviewer’s report  
 
Title: Rapidly declining trend of signet ring cell cancer of the stomach may parallel the 
infection rate of Helicobacter pylori  
 
Version: 1 Date: 07 Sep 2019  
 
Reviewer: Giovanni Mario Pes  
 
Reviewer's report:  
 
The authors have responded exhaustively and satisfactorily to all my concerns, and added 
new sequence data making the results more robust. They also amended the few mistyping 
errors in the text. I have no other comments and I congratulate for their excelle nt work.  
 
Are the methods appropriate and well described?  
If not, please specify what is required in your comments to the authors.  
 
Yes 
 
Does the work include the necessary controls?  
If not, please specif y which controls are required in your comments to the authors.  
 
Yes 
 
Are the conclusions drawn adequately supported by the data shown?  
If not, please explain in your comments to the authors.  
 
Yes 
 
Are you able to assess any statistics in the manuscript or would you recommend an 
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects require further 
assessment in your comments to the editors.  
 
I am able to assess the statistics  
 
Quality of written English  
Please indicate the quality of language in the manuscript:  
 
Acceptable  
 
Declaration of competing interests  
Please complete a declaration of competing interests, considering the following questions:  
 
1. Have you in the past five years received reimburs ements, fees, funding, or salary from 
an organisation that may in any way gain or lose financially from the publication of 
this manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares in an organisation that may in any way gain or lose 
financially from the publication of this manuscript, either now or in the future?  
 
3. Do you hold or are you currently applying for any patents relating to the content of 
the manuscript?  
 
4. Have you received reimbursements, fees, funding, or salary from an organization that 
holds or has applied for patents relating to the content of the manuscript?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have any non -financial competing interests in relation to this paper?  
 
If you can answer no to all o f the above, write 'I declare that I have no competing interests' 
below.  
If your reply is yes to any, please give details below.  
 
I declare that I have no competing interests  
 
I agree to the open peer review policy of the journal. I understand that my name  will be 
included on my report to the authors and, if the manuscript is accepted for publication, my 
named report including any attachments I upload will be posted on the website along with the 
authors' responses. I agree for my report to be made available  under an Open Access Creative 
Commons CC -BY license ( http://creativecommons.org/licenses/by/4.0/ ). I understand that 
any comments which I do not wish to be included in my named report can be incl uded as 
confidential comments to the editors, which will not be published.  
 
I agree to the open peer review policy of the journal  
 
 
Please review this paper.
user
Reviewer’s report  
 
Title: Rapidly declining trend of signet ring cell cancer of the stomach may parallel the 
infection rate of Helicobacter pylori  
 
Version: 1 Date: 07 Sep 2019  
 
Reviewer: Giovanni Mario Pes  
 
Reviewer's report:  
 
The authors have responded exhaustively and satisfactorily to all my concerns, and added 
new sequence data making the results more robust. They also amended the few mistyping 
errors in the text. I have no other comments and I congratulate for their excelle nt work.  
 
Are the methods appropriate and well described?  
If not, please specify what is required in your comments to the authors.  
 
Yes 
 
Does the work include the necessary controls?  
If not, please specif y which controls are required in your comments to the authors.  
 
Yes 
 
Are the conclusions drawn adequately supported by the data shown?  
If not, please explain in your comments to the authors.  
 
Yes 
 
Are you able to assess any statistics in the manuscript or would you recommend an 
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects require further 
assessment in your comments to the editors.  
 
I am able to assess the statistics  
 
Quality of written English  
Please indicate the quality of language in the manuscript:  
 
Acceptable  
 
Declaration of competing interests  
Please complete a declaration of competing interests, considering the following questions:  
 
1. Have you in the past five years received reimburs ements, fees, funding, or salary from 
an organisation that may in any way gain or lose financially from the publication of 
this manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares in an organisation that may in any way gain or lose 
financially from the publication of this manuscript, either now or in the future?  
 
3. Do you hold or are you currently applying for any patents relating to the content of 
the manuscript?  
 
4. Have you received reimbursements, fees, funding, or salary from an organization that 
holds or has applied for patents relating to the content of the manuscript?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have any non -financial competing interests in relation to this paper?  
 
If you can answer no to all o f the above, write 'I declare that I have no competing interests' 
below.  
If your reply is yes to any, please give details below.  
 
I declare that I have no competing interests  
 
I agree to the open peer review policy of the journal. I understand that my name  will be 
included on my report to the authors and, if the manuscript is accepted for publication, my 
named report including any attachments I upload will be posted on the website along with the 
authors' responses. I agree for my report to be made available  under an Open Access Creative 
Commons CC -BY license ( http://creativecommons.org/licenses/by/4.0/ ). I understand that 
any comments which I do not wish to be included in my named report can be incl uded as 
confidential comments to the editors, which will not be published.  
 
I agree to the open peer review policy of the journal  
 
 
assistant