Title: Transcriptome sequencing reveals high-salt diet-induced abnormal liver metabolic
pathways in mice
Version: 1 Date: 01 Jun 2021
Reviewer's report:
The study presented is interesting and add novelties to the scientific literature regarding the salt
effects in the metabolism. The paper is well presented and requires only a few observations to
be further improved.
Methods.
1. Why did the authors include female mice in the study? We know that in metabolic-related
studies the use of male mice is preferrable as females have a more accentuated hormone
interference. I suggest to include this justification and limitation in the discussion section.
2. Please provide the diets composition details.
3. I suggest the inclusion of the tissues thickness used for this staining procedure.
4. The biochemical procedure to assess hepatic enzymes activities should be reported and
detailed in the methods section, including the methodology protocol and reagents used.
Results
1. In figure 3, the green indicates up-regulated? In the figure legend it appears to be the opposite.
Green for down-regulated and red for up-regulated. Please clarify.
Conclusions
1. In the conclusion the authors used the term long-term high-salt diet. Please correct this
affirmation. The study presented a short-term treatment, not a long-term high-salt intervention.
In fact, it is an important study that shows early signs of metabolic damage, even after a
short-term intervention, which must be acknowledged, however, further studies are needed to
clarify how these pathways will behave on a long-term high-salt diet. Please comment these
observations, either in the conclusion or discussion sections.
Abbreviations
1. There are abbreviations missing from this list.
Title: Transcriptome sequencing reveals high-salt diet-induced abnormal liver metabolic
pathways in mice
Version: 1 Date: 01 Jun 2021
Reviewer's report:
The study presented is interesting and add novelties to the scientific literature regarding the salt
effects in the metabolism. The paper is well presented and requires only a few observations to
be further improved.
Methods.
1. Why did the authors include female mice in the study? We know that in metabolic-related
studies the use of male mice is preferrable as females have a more accentuated hormone
interference. I suggest to include this justification and limitation in the discussion section.
2. Please provide the diets composition details.
3. I suggest the inclusion of the tissues thickness used for this staining procedure.
4. The biochemical procedure to assess hepatic enzymes activities should be reported and
detailed in the methods section, including the methodology protocol and reagents used.
Results
1. In figure 3, the green indicates up-regulated? In the figure legend it appears to be the opposite.
Green for down-regulated and red for up-regulated. Please clarify.
Conclusions
1. In the conclusion the authors used the term long-term high-salt diet. Please correct this
affirmation. The study presented a short-term treatment, not a long-term high-salt intervention.
In fact, it is an important study that shows early signs of metabolic damage, even after a
short-term intervention, which must be acknowledged, however, further studies are needed to
clarify how these pathways will behave on a long-term high-salt diet. Please comment these
observations, either in the conclusion or discussion sections.
Abbreviations
1. There are abbreviations missing from this list.
Please review this paper.
user
Title: Transcriptome sequencing reveals high-salt diet-induced abnormal liver metabolic
pathways in mice
Version: 1 Date: 01 Jun 2021
Reviewer's report:
The study presented is interesting and add novelties to the scientific literature regarding the salt
effects in the metabolism. The paper is well presented and requires only a few observations to
be further improved.
Methods.
1. Why did the authors include female mice in the study? We know that in metabolic-related
studies the use of male mice is preferrable as females have a more accentuated hormone
interference. I suggest to include this justification and limitation in the discussion section.
2. Please provide the diets composition details.
3. I suggest the inclusion of the tissues thickness used for this staining procedure.
4. The biochemical procedure to assess hepatic enzymes activities should be reported and
detailed in the methods section, including the methodology protocol and reagents used.
Results
1. In figure 3, the green indicates up-regulated? In the figure legend it appears to be the opposite.
Green for down-regulated and red for up-regulated. Please clarify.
Conclusions
1. In the conclusion the authors used the term long-term high-salt diet. Please correct this
affirmation. The study presented a short-term treatment, not a long-term high-salt intervention.
In fact, it is an important study that shows early signs of metabolic damage, even after a
short-term intervention, which must be acknowledged, however, further studies are needed to
clarify how these pathways will behave on a long-term high-salt diet. Please comment these
observations, either in the conclusion or discussion sections.
Abbreviations
1. There are abbreviations missing from this list.
assistant