Reviewer's report
Title:Protective effects of hydrogen enriched saline on liver ischemia reperfusion
injury by reducing oxidative stress and HMGB1 release
Version: 1Date:30 May 2013
Reviewer: Keliang Xie
Reviewer's report:
General Comments:
In this study, Liu et al shows that hydrogen enriched saline protects liver
ischemia reperfusion injury by reducing oxidative stress and HMGB1 release.
The paper is clearly written and data is convincing. However, the novelty of the
study is diminished considerably by two publications (Hayashida K, et al.
Inhalation of hydrogen gas reduces infarct size in the rat model of myocardial
ischemia-reperfusion injury. Biochem Biophys Res Commun 2008, 373(1):30-35.;
Xie K, et al. Protective effects of hydrogen gas on murine polymicrobial sepsis
via reducing oxidative stress and HMGB1 release. Shock. 2010, 34(1):90-7.).
Additionally, the authors’ data is descriptive. In conclusion, I think, this work can
be published in this journal. But the following questions should be answered.
Major comments:
1. Normally, HMGB1 is mainly in the nucleus of cells. HMGB1 can be actively
secreted by activated macrophages or passively released from necrotic tissues.
In this study, did hydrogen enriched saline decrease the HMGB1 level through
inhibition of active secretion or passive release? In addition, how did the
hydrogen enriched saline reduce the HMGB1 levels in liver and serum?
2. In the treatment group, hydrogen enriched saline was used at a dose of 2.5, 5
or 10ml/kg. Why was the dose 3.5 ml/kg in the saline group?
Minor comments:
1. “1% hydrogen enriched saline” is not right. It should be “hydrogen enriched
saline”.
2. Page 3: HMGB1 is an early mediator of injury. HMGB1 should be a late
mediator.
3. Why was the hydrogen enriched saline used intraperitoneally not
intravenously?
4. Figure 1: Schematic illustration should be more precise. Such as, the time
points of saline administration.
5. The grouping method is not clear. In the figure legends, normal saline or
hydrogen enriched saline is used at dose of 2.5, 5 or 10 ml/kg 10 minutes before
reperfusion. But, hydrogen enriched saline is used 10 minutes before
reperfusion, and at 2, 6, 12 and 20 hours after reperfusion in grouping method.
6. The unit of Y-axis in Figure 4 is?
7. In figures, the X-axis “2h, 6, 12, 24” should be “2h, 6h, 12h, 24h”
8. The data of all groups should be offered. Such as, the data of Sham or IR
groups in figure 5.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Reviewer's report
Title:Protective effects of hydrogen enriched saline on liver ischemia reperfusion
injury by reducing oxidative stress and HMGB1 release
Version: 1Date:30 May 2013
Reviewer: Keliang Xie
Reviewer's report:
General Comments:
In this study, Liu et al shows that hydrogen enriched saline protects liver
ischemia reperfusion injury by reducing oxidative stress and HMGB1 release.
The paper is clearly written and data is convincing. However, the novelty of the
study is diminished considerably by two publications (Hayashida K, et al.
Inhalation of hydrogen gas reduces infarct size in the rat model of myocardial
ischemia-reperfusion injury. Biochem Biophys Res Commun 2008, 373(1):30-35.;
Xie K, et al. Protective effects of hydrogen gas on murine polymicrobial sepsis
via reducing oxidative stress and HMGB1 release. Shock. 2010, 34(1):90-7.).
Additionally, the authors’ data is descriptive. In conclusion, I think, this work can
be published in this journal. But the following questions should be answered.
Major comments:
1. Normally, HMGB1 is mainly in the nucleus of cells. HMGB1 can be actively
secreted by activated macrophages or passively released from necrotic tissues.
In this study, did hydrogen enriched saline decrease the HMGB1 level through
inhibition of active secretion or passive release? In addition, how did the
hydrogen enriched saline reduce the HMGB1 levels in liver and serum?
2. In the treatment group, hydrogen enriched saline was used at a dose of 2.5, 5
or 10ml/kg. Why was the dose 3.5 ml/kg in the saline group?
Minor comments:
1. “1% hydrogen enriched saline” is not right. It should be “hydrogen enriched
saline”.
2. Page 3: HMGB1 is an early mediator of injury. HMGB1 should be a late
mediator.
3. Why was the hydrogen enriched saline used intraperitoneally not
intravenously?
4. Figure 1: Schematic illustration should be more precise. Such as, the time
points of saline administration.
5. The grouping method is not clear. In the figure legends, normal saline or
hydrogen enriched saline is used at dose of 2.5, 5 or 10 ml/kg 10 minutes before
reperfusion. But, hydrogen enriched saline is used 10 minutes before
reperfusion, and at 2, 6, 12 and 20 hours after reperfusion in grouping method.
6. The unit of Y-axis in Figure 4 is?
7. In figures, the X-axis “2h, 6, 12, 24” should be “2h, 6h, 12h, 24h”
8. The data of all groups should be offered. Such as, the data of Sham or IR
groups in figure 5.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
Please review this paper.
user
Reviewer's report
Title:Protective effects of hydrogen enriched saline on liver ischemia reperfusion
injury by reducing oxidative stress and HMGB1 release
Version: 1Date:30 May 2013
Reviewer: Keliang Xie
Reviewer's report:
General Comments:
In this study, Liu et al shows that hydrogen enriched saline protects liver
ischemia reperfusion injury by reducing oxidative stress and HMGB1 release.
The paper is clearly written and data is convincing. However, the novelty of the
study is diminished considerably by two publications (Hayashida K, et al.
Inhalation of hydrogen gas reduces infarct size in the rat model of myocardial
ischemia-reperfusion injury. Biochem Biophys Res Commun 2008, 373(1):30-35.;
Xie K, et al. Protective effects of hydrogen gas on murine polymicrobial sepsis
via reducing oxidative stress and HMGB1 release. Shock. 2010, 34(1):90-7.).
Additionally, the authors’ data is descriptive. In conclusion, I think, this work can
be published in this journal. But the following questions should be answered.
Major comments:
1. Normally, HMGB1 is mainly in the nucleus of cells. HMGB1 can be actively
secreted by activated macrophages or passively released from necrotic tissues.
In this study, did hydrogen enriched saline decrease the HMGB1 level through
inhibition of active secretion or passive release? In addition, how did the
hydrogen enriched saline reduce the HMGB1 levels in liver and serum?
2. In the treatment group, hydrogen enriched saline was used at a dose of 2.5, 5
or 10ml/kg. Why was the dose 3.5 ml/kg in the saline group?
Minor comments:
1. “1% hydrogen enriched saline” is not right. It should be “hydrogen enriched
saline”.
2. Page 3: HMGB1 is an early mediator of injury. HMGB1 should be a late
mediator.
3. Why was the hydrogen enriched saline used intraperitoneally not
intravenously?
4. Figure 1: Schematic illustration should be more precise. Such as, the time
points of saline administration.
5. The grouping method is not clear. In the figure legends, normal saline or
hydrogen enriched saline is used at dose of 2.5, 5 or 10 ml/kg 10 minutes before
reperfusion. But, hydrogen enriched saline is used 10 minutes before
reperfusion, and at 2, 6, 12 and 20 hours after reperfusion in grouping method.
6. The unit of Y-axis in Figure 4 is?
7. In figures, the X-axis “2h, 6, 12, 24” should be “2h, 6h, 12h, 24h”
8. The data of all groups should be offered. Such as, the data of Sham or IR
groups in figure 5.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Needs some language corrections before being
published
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests.
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