Title: Bacterial and Clinical Metabolic Signatures and Their Interactions in Obese Patients Post-
Bariatric Surgery
Version: 5 Date: 29 Jul 2024
Reviewer's report:
Thank you for your valuable article and it is my pleasure to review the manuscript of BMC
Gastroenterology " Changes and Effects of Intestinal Microbiota after Laparoscopic Sleeve
Gastrectomy".
In this paper, the authors compared the composition of the intestinal microbiota in obese patients
before and after laparoscopic sleeve gastrectomy (LSG) through 16S rDNA analysis of the stool
samples. In the results, LSG increased the richness and diversity of the intestinal microbiota in
obese patients after surgery.
This paper gives us the important information, however it has some weaknesses. Thus, it needs
to be revised in some respects. I would provide the following comments and queries.
Major Comments
1. In general, weight loss after LSG does not reach a plateau at 6 months after surgery. Why
did you compare the intestinal microbiota before and 6 months after surgery? If you compared
before and 1 year after surgery, you might get a clearer result.
2. In the Methods section, please provide more details about the 16S rDNA Sequencing and
Analysis, or cite a reference.
3. Please explain what indices related to #-diversity analysis are shown in Fig. 2A. Did #-
diversity decrease after LSG?
4. this study conducted Spearman correlation coefficient analysis between post-bariatric surgery
patients' gut microbiota and clinical indicators, as shown in Fig. 4. Is not there any benefit to
conducting Spearman correlation coefficient analysis between pre-bariatric surgery patients' gut
microbiota and clinical indicators?
5. Bypass procedures like the RYGB or DS could be expected to alter the microbiota especially
in the small intestine. Why should we expect to have such changes after a LSG?
6. At the phylum and class level, there are statistically significant changes in the microbial
communities, although some of these appear to be very small changes. Do the authors believe
this will make a clinically significant change?
Title: Bacterial and Clinical Metabolic Signatures and Their Interactions in Obese Patients Post-
Bariatric Surgery
Version: 5 Date: 29 Jul 2024
Reviewer's report:
Thank you for your valuable article and it is my pleasure to review the manuscript of BMC
Gastroenterology " Changes and Effects of Intestinal Microbiota after Laparoscopic Sleeve
Gastrectomy".
In this paper, the authors compared the composition of the intestinal microbiota in obese patients
before and after laparoscopic sleeve gastrectomy (LSG) through 16S rDNA analysis of the stool
samples. In the results, LSG increased the richness and diversity of the intestinal microbiota in
obese patients after surgery.
This paper gives us the important information, however it has some weaknesses. Thus, it needs
to be revised in some respects. I would provide the following comments and queries.
Major Comments
1. In general, weight loss after LSG does not reach a plateau at 6 months after surgery. Why
did you compare the intestinal microbiota before and 6 months after surgery? If you compared
before and 1 year after surgery, you might get a clearer result.
2. In the Methods section, please provide more details about the 16S rDNA Sequencing and
Analysis, or cite a reference.
3. Please explain what indices related to #-diversity analysis are shown in Fig. 2A. Did #-
diversity decrease after LSG?
4. this study conducted Spearman correlation coefficient analysis between post-bariatric surgery
patients' gut microbiota and clinical indicators, as shown in Fig. 4. Is not there any benefit to
conducting Spearman correlation coefficient analysis between pre-bariatric surgery patients' gut
microbiota and clinical indicators?
5. Bypass procedures like the RYGB or DS could be expected to alter the microbiota especially
in the small intestine. Why should we expect to have such changes after a LSG?
6. At the phylum and class level, there are statistically significant changes in the microbial
communities, although some of these appear to be very small changes. Do the authors believe
this will make a clinically significant change?
Please review this paper.
user
Title: Bacterial and Clinical Metabolic Signatures and Their Interactions in Obese Patients Post-
Bariatric Surgery
Version: 5 Date: 29 Jul 2024
Reviewer's report:
Thank you for your valuable article and it is my pleasure to review the manuscript of BMC
Gastroenterology " Changes and Effects of Intestinal Microbiota after Laparoscopic Sleeve
Gastrectomy".
In this paper, the authors compared the composition of the intestinal microbiota in obese patients
before and after laparoscopic sleeve gastrectomy (LSG) through 16S rDNA analysis of the stool
samples. In the results, LSG increased the richness and diversity of the intestinal microbiota in
obese patients after surgery.
This paper gives us the important information, however it has some weaknesses. Thus, it needs
to be revised in some respects. I would provide the following comments and queries.
Major Comments
1. In general, weight loss after LSG does not reach a plateau at 6 months after surgery. Why
did you compare the intestinal microbiota before and 6 months after surgery? If you compared
before and 1 year after surgery, you might get a clearer result.
2. In the Methods section, please provide more details about the 16S rDNA Sequencing and
Analysis, or cite a reference.
3. Please explain what indices related to #-diversity analysis are shown in Fig. 2A. Did #-
diversity decrease after LSG?
4. this study conducted Spearman correlation coefficient analysis between post-bariatric surgery
patients' gut microbiota and clinical indicators, as shown in Fig. 4. Is not there any benefit to
conducting Spearman correlation coefficient analysis between pre-bariatric surgery patients' gut
microbiota and clinical indicators?
5. Bypass procedures like the RYGB or DS could be expected to alter the microbiota especially
in the small intestine. Why should we expect to have such changes after a LSG?
6. At the phylum and class level, there are statistically significant changes in the microbial
communities, although some of these appear to be very small changes. Do the authors believe
this will make a clinically significant change?
assistant