Title: Effect and potential mechanism of oncometabolite succinate promotes distant metastasis
of colorectal cancer by activating STAT3
Version: 2 Date: 02 Oct 2023
Reviewer's report:
The authors report that succinate could significantly enhance the metastasis and invasion of
colorectal cancer cells and enhance p-STAT3 expression and the EMT process.
I recommend to address following points for the article more informative.
We would like to inquire if there are any results available regarding the analysis of succinate
expression levels in clinical samples and its correlation with prognosis or distant metastasis.
The text states that an increase in succinate concentration leads to a decrease in cell viability
due to the effects of drug toxicity. Kindly, share the supporting evidence.
We would like to know if experiments have been performed to confirm the link between
metabolic pathways, cell cycle, and cell death.
In vivo experiments, it was confirmed that succinate enhances the distant metastasis of colorectal
cancer. We are interested in the effects when inhibiting the phosphorylation of STAT3.
You are conducting in vivo experiments to study the promotion of distant metastasis in colorectal
cancer.
Please let us know if there is a specific reason why you evaluated lung metastasis rather than
liver metastasis.
The notation regarding the presence or absence of significance in the comparison between the
two groups is missing.
There are some spelling mistakes in the text, so please check again.
Title: Effect and potential mechanism of oncometabolite succinate promotes distant metastasis
of colorectal cancer by activating STAT3
Version: 2 Date: 02 Oct 2023
Reviewer's report:
The authors report that succinate could significantly enhance the metastasis and invasion of
colorectal cancer cells and enhance p-STAT3 expression and the EMT process.
I recommend to address following points for the article more informative.
We would like to inquire if there are any results available regarding the analysis of succinate
expression levels in clinical samples and its correlation with prognosis or distant metastasis.
The text states that an increase in succinate concentration leads to a decrease in cell viability
due to the effects of drug toxicity. Kindly, share the supporting evidence.
We would like to know if experiments have been performed to confirm the link between
metabolic pathways, cell cycle, and cell death.
In vivo experiments, it was confirmed that succinate enhances the distant metastasis of colorectal
cancer. We are interested in the effects when inhibiting the phosphorylation of STAT3.
You are conducting in vivo experiments to study the promotion of distant metastasis in colorectal
cancer.
Please let us know if there is a specific reason why you evaluated lung metastasis rather than
liver metastasis.
The notation regarding the presence or absence of significance in the comparison between the
two groups is missing.
There are some spelling mistakes in the text, so please check again.
Please review this paper.
user
Title: Effect and potential mechanism of oncometabolite succinate promotes distant metastasis
of colorectal cancer by activating STAT3
Version: 2 Date: 02 Oct 2023
Reviewer's report:
The authors report that succinate could significantly enhance the metastasis and invasion of
colorectal cancer cells and enhance p-STAT3 expression and the EMT process.
I recommend to address following points for the article more informative.
We would like to inquire if there are any results available regarding the analysis of succinate
expression levels in clinical samples and its correlation with prognosis or distant metastasis.
The text states that an increase in succinate concentration leads to a decrease in cell viability
due to the effects of drug toxicity. Kindly, share the supporting evidence.
We would like to know if experiments have been performed to confirm the link between
metabolic pathways, cell cycle, and cell death.
In vivo experiments, it was confirmed that succinate enhances the distant metastasis of colorectal
cancer. We are interested in the effects when inhibiting the phosphorylation of STAT3.
You are conducting in vivo experiments to study the promotion of distant metastasis in colorectal
cancer.
Please let us know if there is a specific reason why you evaluated lung metastasis rather than
liver metastasis.
The notation regarding the presence or absence of significance in the comparison between the
two groups is missing.
There are some spelling mistakes in the text, so please check again.
assistant