Reviewer's report
Title:Influence of acute pancreatitis on the responsiveness of rat mesenteric and
pulmonary arteries
Version: 1Date:20 February 2008
Reviewer: Daniel Closa
Reviewer's report:
The paper entitled â##Influence of acute pancreatitis on the responsiveness of
rat mesenteric and pulmonary arteriesâ## from Camargo EA et al. reports on the
effect of two different models of experimental cute pancreatitis on the relaxing
and contractile responses of mesenteric or pulmonary artery rings. Authors
observed that, the potency of acetylcholine was decreased in mesenteric but not
in pulmonary rings. No changes were observed in response to SNP and the
potency for PHE was decreased in both mesenteric and pulmonary rings. It was
also observed marked increases in NOx- levels after induction of pancreatitis.
Authors concluded that the changes in response to Ach were associated with
high plasma NOx- levels and that the subsensitivity of contractile response to
PHE migh be involved in the complications of acute pancreatitis.
Major revisions
1. The main problem is the conclusion that links the NOx- levels observed in
plasma with the changes observed in response to PHE. Reports from other
disorders suggests that this could be an explanation, but there is no a direct
demonstration in this work that allows to conclude the link between the two
phenomena. The use of an inhibitor of NOS could be an easy way to
demonstrate this hypothesis or, alternatively, conclusions can be modiffied.
Minor revisions
1. Authors used two different models of pancreatitis. Taurocholate-induced
pancreatitis is a usual model to induce severe pancreatitis, but administration of
PLA2 form Naja mocambique is less common. The rationale for using two
models, and in particular the second one must be explained.
2. In background, authors indicate that â##acute pancreatitis is a disease
characterized by activation of pancreatic enzymes, with severe tissue damage,
local inflammatory reaction, haemorrhage that is associated with remote organ
failure, sepsis and a high prevalence of mortalityâ##. This definition only applies
to the severe forms of the disease. This fat needs to be pointed out.
3. Despite models of pancreatitis are well established, a marker of severity
(lipase, amylase,â#¦) must be included in the experimental design.
What next?: Unable to decide on acceptance or rejection until the authors have
responded to the major compulsory revisions
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Influence of acute pancreatitis on the responsiveness of rat mesenteric and
pulmonary arteries
Version: 1Date:20 February 2008
Reviewer: Daniel Closa
Reviewer's report:
The paper entitled â##Influence of acute pancreatitis on the responsiveness of
rat mesenteric and pulmonary arteriesâ## from Camargo EA et al. reports on the
effect of two different models of experimental cute pancreatitis on the relaxing
and contractile responses of mesenteric or pulmonary artery rings. Authors
observed that, the potency of acetylcholine was decreased in mesenteric but not
in pulmonary rings. No changes were observed in response to SNP and the
potency for PHE was decreased in both mesenteric and pulmonary rings. It was
also observed marked increases in NOx- levels after induction of pancreatitis.
Authors concluded that the changes in response to Ach were associated with
high plasma NOx- levels and that the subsensitivity of contractile response to
PHE migh be involved in the complications of acute pancreatitis.
Major revisions
1. The main problem is the conclusion that links the NOx- levels observed in
plasma with the changes observed in response to PHE. Reports from other
disorders suggests that this could be an explanation, but there is no a direct
demonstration in this work that allows to conclude the link between the two
phenomena. The use of an inhibitor of NOS could be an easy way to
demonstrate this hypothesis or, alternatively, conclusions can be modiffied.
Minor revisions
1. Authors used two different models of pancreatitis. Taurocholate-induced
pancreatitis is a usual model to induce severe pancreatitis, but administration of
PLA2 form Naja mocambique is less common. The rationale for using two
models, and in particular the second one must be explained.
2. In background, authors indicate that â##acute pancreatitis is a disease
characterized by activation of pancreatic enzymes, with severe tissue damage,
local inflammatory reaction, haemorrhage that is associated with remote organ
failure, sepsis and a high prevalence of mortalityâ##. This definition only applies
to the severe forms of the disease. This fat needs to be pointed out.
3. Despite models of pancreatitis are well established, a marker of severity
(lipase, amylase,â#¦) must be included in the experimental design.
What next?: Unable to decide on acceptance or rejection until the authors have
responded to the major compulsory revisions
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Influence of acute pancreatitis on the responsiveness of rat mesenteric and
pulmonary arteries
Version: 1Date:20 February 2008
Reviewer: Daniel Closa
Reviewer's report:
The paper entitled â##Influence of acute pancreatitis on the responsiveness of
rat mesenteric and pulmonary arteriesâ## from Camargo EA et al. reports on the
effect of two different models of experimental cute pancreatitis on the relaxing
and contractile responses of mesenteric or pulmonary artery rings. Authors
observed that, the potency of acetylcholine was decreased in mesenteric but not
in pulmonary rings. No changes were observed in response to SNP and the
potency for PHE was decreased in both mesenteric and pulmonary rings. It was
also observed marked increases in NOx- levels after induction of pancreatitis.
Authors concluded that the changes in response to Ach were associated with
high plasma NOx- levels and that the subsensitivity of contractile response to
PHE migh be involved in the complications of acute pancreatitis.
Major revisions
1. The main problem is the conclusion that links the NOx- levels observed in
plasma with the changes observed in response to PHE. Reports from other
disorders suggests that this could be an explanation, but there is no a direct
demonstration in this work that allows to conclude the link between the two
phenomena. The use of an inhibitor of NOS could be an easy way to
demonstrate this hypothesis or, alternatively, conclusions can be modiffied.
Minor revisions
1. Authors used two different models of pancreatitis. Taurocholate-induced
pancreatitis is a usual model to induce severe pancreatitis, but administration of
PLA2 form Naja mocambique is less common. The rationale for using two
models, and in particular the second one must be explained.
2. In background, authors indicate that â##acute pancreatitis is a disease
characterized by activation of pancreatic enzymes, with severe tissue damage,
local inflammatory reaction, haemorrhage that is associated with remote organ
failure, sepsis and a high prevalence of mortalityâ##. This definition only applies
to the severe forms of the disease. This fat needs to be pointed out.
3. Despite models of pancreatitis are well established, a marker of severity
(lipase, amylase,â#¦) must be included in the experimental design.
What next?: Unable to decide on acceptance or rejection until the authors have
responded to the major compulsory revisions
Level of interest: An article of importance in its field
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I declare that I have no competing interests
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