Reviewer's report
Title:The influence of astragalus polysaccharide and beta-elemene on LX-2 cell
growth, apoptosis and activation
Version:2Date:26 August 2014
Reviewer: Hua wang
Reviewer's report:
This manuscript by Ming-hui Yang’s group addressed the Single
use of astragalus polysaccharide (APS) or #-elemene (ELE) can inhibit LX-2 cell
proliferation and induce apoptosis. APS+ELE treatment showed higher efficiency
in inducing LX-2 cell apoptosis. The inhibition of mRNA and protein levels of
#-SMA and CD44 induced by TGF-# on LX-2 cells can explain the anti-fibrotic
effect of APS and ELE in treating liver diseases.
Overall, this is interesting research. Most of experiments in the study are
adequately designed and the methods section is adequately explained.
Appropriate references have been cited. However, there are some concerns as
listed below.
In the section of Results, the Schematic diagram illustrating the mechanism of
the whole story should be included.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: Yes, and I have assessed the statistics in my report.
Reviewer's report
Title:The influence of astragalus polysaccharide and beta-elemene on LX-2 cell
growth, apoptosis and activation
Version:2Date:26 August 2014
Reviewer: Hua wang
Reviewer's report:
This manuscript by Ming-hui Yang’s group addressed the Single
use of astragalus polysaccharide (APS) or #-elemene (ELE) can inhibit LX-2 cell
proliferation and induce apoptosis. APS+ELE treatment showed higher efficiency
in inducing LX-2 cell apoptosis. The inhibition of mRNA and protein levels of
#-SMA and CD44 induced by TGF-# on LX-2 cells can explain the anti-fibrotic
effect of APS and ELE in treating liver diseases.
Overall, this is interesting research. Most of experiments in the study are
adequately designed and the methods section is adequately explained.
Appropriate references have been cited. However, there are some concerns as
listed below.
In the section of Results, the Schematic diagram illustrating the mechanism of
the whole story should be included.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: Yes, and I have assessed the statistics in my report.
Please review this paper.
user
Reviewer's report
Title:The influence of astragalus polysaccharide and beta-elemene on LX-2 cell
growth, apoptosis and activation
Version:2Date:26 August 2014
Reviewer: Hua wang
Reviewer's report:
This manuscript by Ming-hui Yang’s group addressed the Single
use of astragalus polysaccharide (APS) or #-elemene (ELE) can inhibit LX-2 cell
proliferation and induce apoptosis. APS+ELE treatment showed higher efficiency
in inducing LX-2 cell apoptosis. The inhibition of mRNA and protein levels of
#-SMA and CD44 induced by TGF-# on LX-2 cells can explain the anti-fibrotic
effect of APS and ELE in treating liver diseases.
Overall, this is interesting research. Most of experiments in the study are
adequately designed and the methods section is adequately explained.
Appropriate references have been cited. However, there are some concerns as
listed below.
In the section of Results, the Schematic diagram illustrating the mechanism of
the whole story should be included.
Level of interest: An article of importance in its field
Quality of written English: Needs some language corrections before being
published
Statistical review: Yes, and I have assessed the statistics in my report.
assistant