Reviewer's report
Title:Age-of-onset-dependent Influence of NOD2/CARD15 gene variants on
Disease behaviour and treatment in Crohn's disease
Version: 2Date:14 January 2013
Reviewer: Francis Vasseur
Reviewer's report:
The manuscript entitled " Age-of-onset-dependent Influence of NOD2/CARD15
gene variants on Disease behaviour and treatment in Crohn's disease" submitted
by Carsten Posovszky et al. aims at evaluating the influence of NOD2 gene
variants in disease behavior and treatment of pediatric and adult-onset Crohn’s
disease, has been amended by the authors.
The questions suggestions and comments have been taken into account by the
authors. Some Minor Essential Revisions remain.
#1 In the sentence "In this retrospective single center study in a tertiary center we
report high prevalence of patients with NOD2 variant alleles with 44% in the
pediatric- and 42% in adult-onset CD. The frequency of NOD2 variants in our
cohort is similar to other European pediatric multicenter cohort studies with a
prevalence of 35 upto 45.6% {Lacher, 2010 #19;de Ridder, 2007 #72;Sun, 2003
#82}." The first part of the sentence reports a frequency of patients and the
second part of the sentence (beginning with " The frequency of NOD2
variants...") sounds like an allelic frequency although it seems a genotype
frequency. This kind of confusion should be corrected.
#2 The previous confusion in mind of the authors as reported in my #1 is evident
in the authors response letter. To the previous #41 they answered " Indeed, we
compared the NOD2 variant allele frequency but we did not found a statistical
difference between the two groups." and refered to allelic frequency although
they seem to mean "patients with variant allele?", indeed in the "change in the
manuscript" they now use a correct formulation with " the presence of two NOD2
mutant allele was found more frequent in the pediatric-onset CD patients" which
refers to patients and thus to genotype frequencies and not allelic frequencies.
The authors must be aware of this mistakes and should not confoung between
allelic and genotype frequencies.
#3 In the legend of supplemental figure 1, why did the authors do not add the
word "allele" to the "patients with NOD2 variant" although they wrote "NOD2
wild-type alleles" just after?
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
Declaration of competing interests:
I declare that I have no competing interests
Reviewer's report
Title:Age-of-onset-dependent Influence of NOD2/CARD15 gene variants on
Disease behaviour and treatment in Crohn's disease
Version: 2Date:14 January 2013
Reviewer: Francis Vasseur
Reviewer's report:
The manuscript entitled " Age-of-onset-dependent Influence of NOD2/CARD15
gene variants on Disease behaviour and treatment in Crohn's disease" submitted
by Carsten Posovszky et al. aims at evaluating the influence of NOD2 gene
variants in disease behavior and treatment of pediatric and adult-onset Crohn’s
disease, has been amended by the authors.
The questions suggestions and comments have been taken into account by the
authors. Some Minor Essential Revisions remain.
#1 In the sentence "In this retrospective single center study in a tertiary center we
report high prevalence of patients with NOD2 variant alleles with 44% in the
pediatric- and 42% in adult-onset CD. The frequency of NOD2 variants in our
cohort is similar to other European pediatric multicenter cohort studies with a
prevalence of 35 upto 45.6% {Lacher, 2010 #19;de Ridder, 2007 #72;Sun, 2003
#82}." The first part of the sentence reports a frequency of patients and the
second part of the sentence (beginning with " The frequency of NOD2
variants...") sounds like an allelic frequency although it seems a genotype
frequency. This kind of confusion should be corrected.
#2 The previous confusion in mind of the authors as reported in my #1 is evident
in the authors response letter. To the previous #41 they answered " Indeed, we
compared the NOD2 variant allele frequency but we did not found a statistical
difference between the two groups." and refered to allelic frequency although
they seem to mean "patients with variant allele?", indeed in the "change in the
manuscript" they now use a correct formulation with " the presence of two NOD2
mutant allele was found more frequent in the pediatric-onset CD patients" which
refers to patients and thus to genotype frequencies and not allelic frequencies.
The authors must be aware of this mistakes and should not confoung between
allelic and genotype frequencies.
#3 In the legend of supplemental figure 1, why did the authors do not add the
word "allele" to the "patients with NOD2 variant" although they wrote "NOD2
wild-type alleles" just after?
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
Declaration of competing interests:
I declare that I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Age-of-onset-dependent Influence of NOD2/CARD15 gene variants on
Disease behaviour and treatment in Crohn's disease
Version: 2Date:14 January 2013
Reviewer: Francis Vasseur
Reviewer's report:
The manuscript entitled " Age-of-onset-dependent Influence of NOD2/CARD15
gene variants on Disease behaviour and treatment in Crohn's disease" submitted
by Carsten Posovszky et al. aims at evaluating the influence of NOD2 gene
variants in disease behavior and treatment of pediatric and adult-onset Crohn’s
disease, has been amended by the authors.
The questions suggestions and comments have been taken into account by the
authors. Some Minor Essential Revisions remain.
#1 In the sentence "In this retrospective single center study in a tertiary center we
report high prevalence of patients with NOD2 variant alleles with 44% in the
pediatric- and 42% in adult-onset CD. The frequency of NOD2 variants in our
cohort is similar to other European pediatric multicenter cohort studies with a
prevalence of 35 upto 45.6% {Lacher, 2010 #19;de Ridder, 2007 #72;Sun, 2003
#82}." The first part of the sentence reports a frequency of patients and the
second part of the sentence (beginning with " The frequency of NOD2
variants...") sounds like an allelic frequency although it seems a genotype
frequency. This kind of confusion should be corrected.
#2 The previous confusion in mind of the authors as reported in my #1 is evident
in the authors response letter. To the previous #41 they answered " Indeed, we
compared the NOD2 variant allele frequency but we did not found a statistical
difference between the two groups." and refered to allelic frequency although
they seem to mean "patients with variant allele?", indeed in the "change in the
manuscript" they now use a correct formulation with " the presence of two NOD2
mutant allele was found more frequent in the pediatric-onset CD patients" which
refers to patients and thus to genotype frequencies and not allelic frequencies.
The authors must be aware of this mistakes and should not confoung between
allelic and genotype frequencies.
#3 In the legend of supplemental figure 1, why did the authors do not add the
word "allele" to the "patients with NOD2 variant" although they wrote "NOD2
wild-type alleles" just after?
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: Yes, and I have assessed the statistics in my report.
Declaration of competing interests:
I declare that I have no competing interests
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