Title: Protective role of thymoquinone in hyperlipidemia-induced liver injury in LDL-R -/- mice
Version: 1 Date: 23 Feb 2023
Reviewer's report:
Dear editor of BMC Gastroenterology below is my opinion of the manuscript.
I suggest that add at least one type of method to the keywords.
Materials and Methods
In general, this research applied many related methods that are very good, but the writing in
this part is very brief.
- Why you didn't use the normal mice that make results more logical and beneficial?
-  Did you prepare the high-cholesterol diet dally or was it ready-made? or what?
-  Is there a reference for the period of animal treatment?
-  Please, add a reference for the euthanized method that you used
-  Why you did not measure the HDL?
-  Please, give a reference for the standard protocol of H&E tissue preparation or write it in
more detail.
-  Please, put the explanation of NLRP3 below table 1 as well as write the complete words of
F and R or explain them below the table.
- Were you applying the same secondary antibody of all parameters in the western blot
technique?
Results (also, the writing in this part is very brief)
- What is the significance level in the hepatic biochemical parameters and metabolic
characteristics among groups? All were smaller than 0.05 or what?
- In the H&E histology pictures, the changes are not clear. You should point to changes with
arrows or letters or else.
- (The HD + TQ group exhibited a markedly reduced accumulation of CD68-positive cells in
the LT compared with the ND group. There was no variation with respect to the aforementioned
parameters in the ND group.) This part needs more revision.
Discussion
Why did body weight decrease in the HD group?
- This part is very good as a comparison, but you do not give any explanation of the results that
may be related to Thymoquinone components. You may find that in some previous research. So,
I encourage you to continue working on this point in corporation with biochemistry scientists.
Title: Protective role of thymoquinone in hyperlipidemia-induced liver injury in LDL-R -/- mice
Version: 1 Date: 23 Feb 2023
Reviewer's report:
Dear editor of BMC Gastroenterology below is my opinion of the manuscript.
I suggest that add at least one type of method to the keywords.
Materials and Methods
In general, this research applied many related methods that are very good, but the writing in
this part is very brief.
- Why you didn't use the normal mice that make results more logical and beneficial?
-  Did you prepare the high-cholesterol diet dally or was it ready-made? or what?
-  Is there a reference for the period of animal treatment?
-  Please, add a reference for the euthanized method that you used
-  Why you did not measure the HDL?
-  Please, give a reference for the standard protocol of H&E tissue preparation or write it in
more detail.
-  Please, put the explanation of NLRP3 below table 1 as well as write the complete words of
F and R or explain them below the table.
- Were you applying the same secondary antibody of all parameters in the western blot
technique?
Results (also, the writing in this part is very brief)
- What is the significance level in the hepatic biochemical parameters and metabolic
characteristics among groups? All were smaller than 0.05 or what?
- In the H&E histology pictures, the changes are not clear. You should point to changes with
arrows or letters or else.
- (The HD + TQ group exhibited a markedly reduced accumulation of CD68-positive cells in
the LT compared with the ND group. There was no variation with respect to the aforementioned
parameters in the ND group.) This part needs more revision.
Discussion
Why did body weight decrease in the HD group?
- This part is very good as a comparison, but you do not give any explanation of the results that
may be related to Thymoquinone components. You may find that in some previous research. So,
I encourage you to continue working on this point in corporation with biochemistry scientists.
Please review this paper.
user
Title: Protective role of thymoquinone in hyperlipidemia-induced liver injury in LDL-R -/- mice
Version: 1 Date: 23 Feb 2023
Reviewer's report:
Dear editor of BMC Gastroenterology below is my opinion of the manuscript.
I suggest that add at least one type of method to the keywords.
Materials and Methods
In general, this research applied many related methods that are very good, but the writing in
this part is very brief.
- Why you didn't use the normal mice that make results more logical and beneficial?
-  Did you prepare the high-cholesterol diet dally or was it ready-made? or what?
-  Is there a reference for the period of animal treatment?
-  Please, add a reference for the euthanized method that you used
-  Why you did not measure the HDL?
-  Please, give a reference for the standard protocol of H&E tissue preparation or write it in
more detail.
-  Please, put the explanation of NLRP3 below table 1 as well as write the complete words of
F and R or explain them below the table.
- Were you applying the same secondary antibody of all parameters in the western blot
technique?
Results (also, the writing in this part is very brief)
- What is the significance level in the hepatic biochemical parameters and metabolic
characteristics among groups? All were smaller than 0.05 or what?
- In the H&E histology pictures, the changes are not clear. You should point to changes with
arrows or letters or else.
- (The HD + TQ group exhibited a markedly reduced accumulation of CD68-positive cells in
the LT compared with the ND group. There was no variation with respect to the aforementioned
parameters in the ND group.) This part needs more revision.
Discussion
Why did body weight decrease in the HD group?
- This part is very good as a comparison, but you do not give any explanation of the results that
may be related to Thymoquinone components. You may find that in some previous research. So,
I encourage you to continue working on this point in corporation with biochemistry scientists.
assistant