Title: The expression and clinical significance of CFAP65 in colon cancer
Version: 2 Date: 17 Apr 2024
Reviewer:  Pramod Kumar Tiwari
Reviewer's report:
The revised version of the manuscript has tried to justify most of the questions raised under
comments with fair degree of satisfaction. However, there still remains a few other points to be
considered appropriately. The authors are suggested to work out on the following for improving
the quality of the manuscript.
1. Were beta-actin bands taken from the same gel for each of the samples or taken from some
other gels and, if all the samples, 1-6, loaded together in the same gel or in different gels? The
intensities of paired actin bands seem to be varying.
2. In order to make a true representative of the total sample size or number of patients analyzed
(n=189), the number of samples used for western blot should have been appropriately increased.
This could also give statistical strength to the result.
3. In my view, for validation of the result, RT-PCR results can provide conclusive support,
including the results of western blot. Properly frozen samples can be used for both RT-PCR and
western blots.
Title: The expression and clinical significance of CFAP65 in colon cancer
Version: 2 Date: 17 Apr 2024
Reviewer:  Pramod Kumar Tiwari
Reviewer's report:
The revised version of the manuscript has tried to justify most of the questions raised under
comments with fair degree of satisfaction. However, there still remains a few other points to be
considered appropriately. The authors are suggested to work out on the following for improving
the quality of the manuscript.
1. Were beta-actin bands taken from the same gel for each of the samples or taken from some
other gels and, if all the samples, 1-6, loaded together in the same gel or in different gels? The
intensities of paired actin bands seem to be varying.
2. In order to make a true representative of the total sample size or number of patients analyzed
(n=189), the number of samples used for western blot should have been appropriately increased.
This could also give statistical strength to the result.
3. In my view, for validation of the result, RT-PCR results can provide conclusive support,
including the results of western blot. Properly frozen samples can be used for both RT-PCR and
western blots.
Please review this paper.
user
Title: The expression and clinical significance of CFAP65 in colon cancer
Version: 2 Date: 17 Apr 2024
Reviewer:  Pramod Kumar Tiwari
Reviewer's report:
The revised version of the manuscript has tried to justify most of the questions raised under
comments with fair degree of satisfaction. However, there still remains a few other points to be
considered appropriately. The authors are suggested to work out on the following for improving
the quality of the manuscript.
1. Were beta-actin bands taken from the same gel for each of the samples or taken from some
other gels and, if all the samples, 1-6, loaded together in the same gel or in different gels? The
intensities of paired actin bands seem to be varying.
2. In order to make a true representative of the total sample size or number of patients analyzed
(n=189), the number of samples used for western blot should have been appropriately increased.
This could also give statistical strength to the result.
3. In my view, for validation of the result, RT-PCR results can provide conclusive support,
including the results of western blot. Properly frozen samples can be used for both RT-PCR and
western blots.
assistant