Reviewer's report
Title:Mouse model of carbon tetrachloride induced liver fibrosis:
Histopathological changes and expression of CD133 and epidermal growth factor
Version: 2Date:10 May 2010
Reviewer: helene gilgenkrantz
Reviewer's report:
1. The new data showing CD133 and a-SMA immunohistochemistry in the same
area on serial sections are not really convincing. Particularly a-SMA staining
looks as an artefact in injured cells or area. Therefore it should only be indicated
that « CD133 positive cells could probably correspond to HSC ».
2. The new data showing phosphorylated EGFR expression shows a clear
up-regulation - in contrast to what has been seen with the non –phosphorylated
form of EGFR-. It is in correlation with PCNA expression and therefore probably
with cell proliferation. The discussion has bo be improved on this point.
3. Recent evidences suggest that Amhiregulin, that has been found up-regulated
in the new version, plays a unique role not only in liver fibrosis but also in liver
tumorigenesis and in the maintenance of the neoplastic phenotype of
hepatocarcinoma cells, particularly in humans. This point gives some clinical
relevance to the model developed here.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I have no competing interests
Reviewer's report
Title:Mouse model of carbon tetrachloride induced liver fibrosis:
Histopathological changes and expression of CD133 and epidermal growth factor
Version: 2Date:10 May 2010
Reviewer: helene gilgenkrantz
Reviewer's report:
1. The new data showing CD133 and a-SMA immunohistochemistry in the same
area on serial sections are not really convincing. Particularly a-SMA staining
looks as an artefact in injured cells or area. Therefore it should only be indicated
that « CD133 positive cells could probably correspond to HSC ».
2. The new data showing phosphorylated EGFR expression shows a clear
up-regulation - in contrast to what has been seen with the non –phosphorylated
form of EGFR-. It is in correlation with PCNA expression and therefore probably
with cell proliferation. The discussion has bo be improved on this point.
3. Recent evidences suggest that Amhiregulin, that has been found up-regulated
in the new version, plays a unique role not only in liver fibrosis but also in liver
tumorigenesis and in the maintenance of the neoplastic phenotype of
hepatocarcinoma cells, particularly in humans. This point gives some clinical
relevance to the model developed here.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I have no competing interests
Please review this paper.
user
Reviewer's report
Title:Mouse model of carbon tetrachloride induced liver fibrosis:
Histopathological changes and expression of CD133 and epidermal growth factor
Version: 2Date:10 May 2010
Reviewer: helene gilgenkrantz
Reviewer's report:
1. The new data showing CD133 and a-SMA immunohistochemistry in the same
area on serial sections are not really convincing. Particularly a-SMA staining
looks as an artefact in injured cells or area. Therefore it should only be indicated
that « CD133 positive cells could probably correspond to HSC ».
2. The new data showing phosphorylated EGFR expression shows a clear
up-regulation - in contrast to what has been seen with the non –phosphorylated
form of EGFR-. It is in correlation with PCNA expression and therefore probably
with cell proliferation. The discussion has bo be improved on this point.
3. Recent evidences suggest that Amhiregulin, that has been found up-regulated
in the new version, plays a unique role not only in liver fibrosis but also in liver
tumorigenesis and in the maintenance of the neoplastic phenotype of
hepatocarcinoma cells, particularly in humans. This point gives some clinical
relevance to the model developed here.
Level of interest: An article whose findings are important to those with closely
related research interests
Quality of written English: Acceptable
Statistical review: No, the manuscript does not need to be seen by a
statistician.
Declaration of competing interests:
I have no competing interests
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