Title: PADI4 promotes epithelial-mesenchymal transition(EMT) in gastric cancer via the
upregulation of interleukin 8
Version: 3 Date: 07 Oct 2021
Reviewer:  Gang Liao
Reviewer's report:
The article reached this conclusion: PADI4 promotes EMT in gastric cancer via the upregulation
of interleukin 8. Although the article is interesting, but it needs attention to the following
comments.
1.The gastric cancer tissues used for Real-time PCR and Western blotting were collected at
Shandong Province Qianfoshan Hospital.GC tissue array used for immunohistochemistry was
obtained commercially from Alenabio. Why the gastric cancer tissues used in these three
experiments are of different sources?
2. Why did the authors choose the two human GC cell lines(SGC-7901, MGC80-3) for the
experiment?
3. The authors only studied that the overexpression of PADI4 up-regulated interleukin 8 in the
human GC cell lines. Therefore the strength of the argument is weak. It is necessary to further
study whether interference with PADI4 down-regulates interleukin 8 in the human GC cell lines.
4. It is recommended to indicate the magnification in these figures(Figure 1 D and E, Figure 2
F, Figure 7 A and C)
Title: PADI4 promotes epithelial-mesenchymal transition(EMT) in gastric cancer via the
upregulation of interleukin 8
Version: 3 Date: 07 Oct 2021
Reviewer:  Gang Liao
Reviewer's report:
The article reached this conclusion: PADI4 promotes EMT in gastric cancer via the upregulation
of interleukin 8. Although the article is interesting, but it needs attention to the following
comments.
1.The gastric cancer tissues used for Real-time PCR and Western blotting were collected at
Shandong Province Qianfoshan Hospital.GC tissue array used for immunohistochemistry was
obtained commercially from Alenabio. Why the gastric cancer tissues used in these three
experiments are of different sources?
2. Why did the authors choose the two human GC cell lines(SGC-7901, MGC80-3) for the
experiment?
3. The authors only studied that the overexpression of PADI4 up-regulated interleukin 8 in the
human GC cell lines. Therefore the strength of the argument is weak. It is necessary to further
study whether interference with PADI4 down-regulates interleukin 8 in the human GC cell lines.
4. It is recommended to indicate the magnification in these figures(Figure 1 D and E, Figure 2
F, Figure 7 A and C)
Please review this paper.
user
Title: PADI4 promotes epithelial-mesenchymal transition(EMT) in gastric cancer via the
upregulation of interleukin 8
Version: 3 Date: 07 Oct 2021
Reviewer:  Gang Liao
Reviewer's report:
The article reached this conclusion: PADI4 promotes EMT in gastric cancer via the upregulation
of interleukin 8. Although the article is interesting, but it needs attention to the following
comments.
1.The gastric cancer tissues used for Real-time PCR and Western blotting were collected at
Shandong Province Qianfoshan Hospital.GC tissue array used for immunohistochemistry was
obtained commercially from Alenabio. Why the gastric cancer tissues used in these three
experiments are of different sources?
2. Why did the authors choose the two human GC cell lines(SGC-7901, MGC80-3) for the
experiment?
3. The authors only studied that the overexpression of PADI4 up-regulated interleukin 8 in the
human GC cell lines. Therefore the strength of the argument is weak. It is necessary to further
study whether interference with PADI4 down-regulates interleukin 8 in the human GC cell lines.
4. It is recommended to indicate the magnification in these figures(Figure 1 D and E, Figure 2
F, Figure 7 A and C)
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