Title: ncRNA-mediated SOX4 overexpression correlates with unfavorable outcomes and
immune infiltration in hepatocellular carcinoma
Version: 3 Date: 02 Jul 2024
Reviewer's report:
The revised version of the manuscript mainly addressed my previous comments and concerns.
Overall, the manuscript is well written and of interests to readers.
However, even if the Authors have correctly re-described the results at lines 183-190, they did
not discuss the finding that obesity and overweight seem to be associated with increased OS
in their analysis, while different studies have described that obesity is associated with reduced
cancer survivorship.
At line 179, I would suggest the Authors to state that “increased SOX4 expression was inversely
associated with OS, progression-free survival, disease-free survival, and relapse-free survival
(Figure 2A–D).”
The titles of figure 2H and 2I are still inverted. The Gender graph should have 6 groups/color
lines and the Race graph only 4.
I thank the Authors for the new provided original WB results with miha cell line and GAPDH
instead of b-Actin. However, also in these images the KDa ladders are not merged together
with the protein bands. Can they provide a merged image which would confirm the correct
identification of the protein?
Title: ncRNA-mediated SOX4 overexpression correlates with unfavorable outcomes and
immune infiltration in hepatocellular carcinoma
Version: 3 Date: 02 Jul 2024
Reviewer's report:
The revised version of the manuscript mainly addressed my previous comments and concerns.
Overall, the manuscript is well written and of interests to readers.
However, even if the Authors have correctly re-described the results at lines 183-190, they did
not discuss the finding that obesity and overweight seem to be associated with increased OS
in their analysis, while different studies have described that obesity is associated with reduced
cancer survivorship.
At line 179, I would suggest the Authors to state that “increased SOX4 expression was inversely
associated with OS, progression-free survival, disease-free survival, and relapse-free survival
(Figure 2A–D).”
The titles of figure 2H and 2I are still inverted. The Gender graph should have 6 groups/color
lines and the Race graph only 4.
I thank the Authors for the new provided original WB results with miha cell line and GAPDH
instead of b-Actin. However, also in these images the KDa ladders are not merged together
with the protein bands. Can they provide a merged image which would confirm the correct
identification of the protein?
Please review this paper.
user
Title: ncRNA-mediated SOX4 overexpression correlates with unfavorable outcomes and
immune infiltration in hepatocellular carcinoma
Version: 3 Date: 02 Jul 2024
Reviewer's report:
The revised version of the manuscript mainly addressed my previous comments and concerns.
Overall, the manuscript is well written and of interests to readers.
However, even if the Authors have correctly re-described the results at lines 183-190, they did
not discuss the finding that obesity and overweight seem to be associated with increased OS
in their analysis, while different studies have described that obesity is associated with reduced
cancer survivorship.
At line 179, I would suggest the Authors to state that “increased SOX4 expression was inversely
associated with OS, progression-free survival, disease-free survival, and relapse-free survival
(Figure 2A–D).”
The titles of figure 2H and 2I are still inverted. The Gender graph should have 6 groups/color
lines and the Race graph only 4.
I thank the Authors for the new provided original WB results with miha cell line and GAPDH
instead of b-Actin. However, also in these images the KDa ladders are not merged together
with the protein bands. Can they provide a merged image which would confirm the correct
identification of the protein?
assistant