Title: Comprehensive analysis of ferroptosis-related long non-coding RNA and its
association with tumor progression and ferroptosis in gastric cancer
Version: 1 Date: 30 Jan 2025
Reviewer's report:
In this study, the authors identified lncRNAs correlated with ferroptosis-related genes in GC
patients using the public database TCGA. Additionally, they extracted eight lncRNAs related to
patient prognosis among these ferroptosis-related lncRNAs, and derived the risk score from their
expression levels and Cox regression coefficients. Finally, they conducted in vitro functional
analyses focusing on HAGLR among these lncRNAs, suggesting that this lncRNA could be
a prognostic factor and potentially a therapeutic target. However, this study lacks scientific
evidence in the following important aspects, and its conclusions are excessive.
1. In Figures 3-5, they state that the risk score derived from the expression levels of the eight
lncRNAs is correlated with prognosis. However, they initially extracted lncRNAs related to
prognosis, and their risk score considered the COX regression coefficients within the same
cohort. It is natural for this score to correlate with prognosis when validated in the same cohort.
Moreover, the cohort used for validation was an intrinsic cohort extracted from the cohort
where the expression of these eight lncRNAs correlated with prognosis, making it predictably
correlated with prognosis. In other words, they need to use an entirely different validation cohort
to evaluate this prognostic risk score properly. Otherwise, at this point, their derived score can
only be said to apply to the 334 cases within TCGA.
2. Among the eight lncRNAs, they conducted in vitro functional analyses on HAGLR. They
showed that targeting HAGLR resulted in the inhibition of proliferation, apoptosis induction,
and migration suppression. At the same time, the authors should evaluate how ferroptosis
changes when targeting HAGLR. Is ferroptosis indeed involved in these tumor suppressions?
Title: Comprehensive analysis of ferroptosis-related long non-coding RNA and its
association with tumor progression and ferroptosis in gastric cancer
Version: 1 Date: 30 Jan 2025
Reviewer's report:
In this study, the authors identified lncRNAs correlated with ferroptosis-related genes in GC
patients using the public database TCGA. Additionally, they extracted eight lncRNAs related to
patient prognosis among these ferroptosis-related lncRNAs, and derived the risk score from their
expression levels and Cox regression coefficients. Finally, they conducted in vitro functional
analyses focusing on HAGLR among these lncRNAs, suggesting that this lncRNA could be
a prognostic factor and potentially a therapeutic target. However, this study lacks scientific
evidence in the following important aspects, and its conclusions are excessive.
1. In Figures 3-5, they state that the risk score derived from the expression levels of the eight
lncRNAs is correlated with prognosis. However, they initially extracted lncRNAs related to
prognosis, and their risk score considered the COX regression coefficients within the same
cohort. It is natural for this score to correlate with prognosis when validated in the same cohort.
Moreover, the cohort used for validation was an intrinsic cohort extracted from the cohort
where the expression of these eight lncRNAs correlated with prognosis, making it predictably
correlated with prognosis. In other words, they need to use an entirely different validation cohort
to evaluate this prognostic risk score properly. Otherwise, at this point, their derived score can
only be said to apply to the 334 cases within TCGA.
2. Among the eight lncRNAs, they conducted in vitro functional analyses on HAGLR. They
showed that targeting HAGLR resulted in the inhibition of proliferation, apoptosis induction,
and migration suppression. At the same time, the authors should evaluate how ferroptosis
changes when targeting HAGLR. Is ferroptosis indeed involved in these tumor suppressions?
Please review this paper.
user
Title: Comprehensive analysis of ferroptosis-related long non-coding RNA and its
association with tumor progression and ferroptosis in gastric cancer
Version: 1 Date: 30 Jan 2025
Reviewer's report:
In this study, the authors identified lncRNAs correlated with ferroptosis-related genes in GC
patients using the public database TCGA. Additionally, they extracted eight lncRNAs related to
patient prognosis among these ferroptosis-related lncRNAs, and derived the risk score from their
expression levels and Cox regression coefficients. Finally, they conducted in vitro functional
analyses focusing on HAGLR among these lncRNAs, suggesting that this lncRNA could be
a prognostic factor and potentially a therapeutic target. However, this study lacks scientific
evidence in the following important aspects, and its conclusions are excessive.
1. In Figures 3-5, they state that the risk score derived from the expression levels of the eight
lncRNAs is correlated with prognosis. However, they initially extracted lncRNAs related to
prognosis, and their risk score considered the COX regression coefficients within the same
cohort. It is natural for this score to correlate with prognosis when validated in the same cohort.
Moreover, the cohort used for validation was an intrinsic cohort extracted from the cohort
where the expression of these eight lncRNAs correlated with prognosis, making it predictably
correlated with prognosis. In other words, they need to use an entirely different validation cohort
to evaluate this prognostic risk score properly. Otherwise, at this point, their derived score can
only be said to apply to the 334 cases within TCGA.
2. Among the eight lncRNAs, they conducted in vitro functional analyses on HAGLR. They
showed that targeting HAGLR resulted in the inhibition of proliferation, apoptosis induction,
and migration suppression. At the same time, the authors should evaluate how ferroptosis
changes when targeting HAGLR. Is ferroptosis indeed involved in these tumor suppressions?
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