Title: A comprehensive computational analysis to explore the importance of SIGLECs in HCC
biology
Version: 1 Date: 08 Dec 2021
Reviewer's report:
In the current manuscript Senbang Yao et al, evaluates the functional significance of SIGLECs
family in HCC . The study use TCGA and GEO database for data analysis and the scientific
message is interesting; however I would raise some concerns:
MAJOR CONCERNS:
1) The authors evaluate mRNA levels in HCC and normal patient of the different SIGLECs
family member, but the results are not so clear. The figures and the text do not specifically
define which member of SIGLECs is differentially expressed. This part of the study should be
improved.
2) The authors make a correlation between the different expression of SIGLECs family with
T cells and B cells infiltration levels in HCC. The paragraph seems to be completely separate
from the other part of the study. The authors should explain better the correlation in the text,
especially in the discussion paragraph.
3) The different expression of SIGLECs family members between differ grade and stage of
tumor is an important feature, but it is very difficult to demonstrate statistically significance of
SIGLECs and tumor grade/stage. Number of samples analyzed for the different groups should
be reported.
4) It is also difficult to accept the definition from the title in which is written “predictive role of
SIGLECs family in HCC patients undergoing sorafenib”. The authors analyze data from patient
with HCC and normal patients. How the authors evaluate the predictive role. This part should
be better explained.
5) Discussion paragraph should be improved.
Title: A comprehensive computational analysis to explore the importance of SIGLECs in HCC
biology
Version: 1 Date: 08 Dec 2021
Reviewer's report:
In the current manuscript Senbang Yao et al, evaluates the functional significance of SIGLECs
family in HCC . The study use TCGA and GEO database for data analysis and the scientific
message is interesting; however I would raise some concerns:
MAJOR CONCERNS:
1) The authors evaluate mRNA levels in HCC and normal patient of the different SIGLECs
family member, but the results are not so clear. The figures and the text do not specifically
define which member of SIGLECs is differentially expressed. This part of the study should be
improved.
2) The authors make a correlation between the different expression of SIGLECs family with
T cells and B cells infiltration levels in HCC. The paragraph seems to be completely separate
from the other part of the study. The authors should explain better the correlation in the text,
especially in the discussion paragraph.
3) The different expression of SIGLECs family members between differ grade and stage of
tumor is an important feature, but it is very difficult to demonstrate statistically significance of
SIGLECs and tumor grade/stage. Number of samples analyzed for the different groups should
be reported.
4) It is also difficult to accept the definition from the title in which is written “predictive role of
SIGLECs family in HCC patients undergoing sorafenib”. The authors analyze data from patient
with HCC and normal patients. How the authors evaluate the predictive role. This part should
be better explained.
5) Discussion paragraph should be improved.
Please review this paper.
user
Title: A comprehensive computational analysis to explore the importance of SIGLECs in HCC
biology
Version: 1 Date: 08 Dec 2021
Reviewer's report:
In the current manuscript Senbang Yao et al, evaluates the functional significance of SIGLECs
family in HCC . The study use TCGA and GEO database for data analysis and the scientific
message is interesting; however I would raise some concerns:
MAJOR CONCERNS:
1) The authors evaluate mRNA levels in HCC and normal patient of the different SIGLECs
family member, but the results are not so clear. The figures and the text do not specifically
define which member of SIGLECs is differentially expressed. This part of the study should be
improved.
2) The authors make a correlation between the different expression of SIGLECs family with
T cells and B cells infiltration levels in HCC. The paragraph seems to be completely separate
from the other part of the study. The authors should explain better the correlation in the text,
especially in the discussion paragraph.
3) The different expression of SIGLECs family members between differ grade and stage of
tumor is an important feature, but it is very difficult to demonstrate statistically significance of
SIGLECs and tumor grade/stage. Number of samples analyzed for the different groups should
be reported.
4) It is also difficult to accept the definition from the title in which is written “predictive role of
SIGLECs family in HCC patients undergoing sorafenib”. The authors analyze data from patient
with HCC and normal patients. How the authors evaluate the predictive role. This part should
be better explained.
5) Discussion paragraph should be improved.
assistant