Title: APC mutation correlated with poor response of immunotherapy in colon cancer
Version: 2 Date: 31 Dec 2022
Reviewer's report:
Authors showed an interesting speculation about APC mutation and the response of
immunotherapy in colon cancer.
However, I think that there are several points to be addressed.
1) It is well known that tumors with microsatellite instable have a lower frequency of APC
mutations, compared with microsatellite stable and CpG island methylator phenotype-low
(many of chromosomal instability phenotype) colorectal cancers.
Authors concluded that the colon cancer patients with APC mutations were associated with poor
response of immunotherapy. I think that they should verify the proportion of the chromosomal
instability phenotype, microsatellite instability phenotype and CpG island methylator phenotype
contained in the group with  APC mutations. I wonder if the response to immunotherapy would
be poor because the MSI phenotype was rarely included.
2) If authors have data about analysis of stroma or microenvironment of tumor, they should
show it. I think that these factors are also deeply involved in the efficacy of immunotherapy.
Title: APC mutation correlated with poor response of immunotherapy in colon cancer
Version: 2 Date: 31 Dec 2022
Reviewer's report:
Authors showed an interesting speculation about APC mutation and the response of
immunotherapy in colon cancer.
However, I think that there are several points to be addressed.
1) It is well known that tumors with microsatellite instable have a lower frequency of APC
mutations, compared with microsatellite stable and CpG island methylator phenotype-low
(many of chromosomal instability phenotype) colorectal cancers.
Authors concluded that the colon cancer patients with APC mutations were associated with poor
response of immunotherapy. I think that they should verify the proportion of the chromosomal
instability phenotype, microsatellite instability phenotype and CpG island methylator phenotype
contained in the group with  APC mutations. I wonder if the response to immunotherapy would
be poor because the MSI phenotype was rarely included.
2) If authors have data about analysis of stroma or microenvironment of tumor, they should
show it. I think that these factors are also deeply involved in the efficacy of immunotherapy.
Please review this paper.
user
Title: APC mutation correlated with poor response of immunotherapy in colon cancer
Version: 2 Date: 31 Dec 2022
Reviewer's report:
Authors showed an interesting speculation about APC mutation and the response of
immunotherapy in colon cancer.
However, I think that there are several points to be addressed.
1) It is well known that tumors with microsatellite instable have a lower frequency of APC
mutations, compared with microsatellite stable and CpG island methylator phenotype-low
(many of chromosomal instability phenotype) colorectal cancers.
Authors concluded that the colon cancer patients with APC mutations were associated with poor
response of immunotherapy. I think that they should verify the proportion of the chromosomal
instability phenotype, microsatellite instability phenotype and CpG island methylator phenotype
contained in the group with  APC mutations. I wonder if the response to immunotherapy would
be poor because the MSI phenotype was rarely included.
2) If authors have data about analysis of stroma or microenvironment of tumor, they should
show it. I think that these factors are also deeply involved in the efficacy of immunotherapy.
assistant