
    agios is no longer developing its second pyruvate kinase-r (pkr) activator, ag-519, and
    withdrew its investigational new drug (ind) application in december 2016
  
['anemia']
['ag-519', 'placebo']

        Inclusion Criteria:

          1. Healthy adult male or female subjects. Female subjects must be of non-childbearing
             potential.

          2. Age 18 to 60 years inclusive at time of consent.

          3. Body mass index (BMI) of ≥18.5 to ≤32.0 kg/m2 or, if outside the range, considered not
             clinically significant by the investigator.

          4. Good state of health (mentally and physically) as indicated by a comprehensive
             clinical assessment (detailed medical history and a complete physical examination),
             ECG and laboratory investigations.

          5. Subjects who are actively non-smokers and have not used other nicotine-containing
             products for at least 12 months prior to the screening assessment.

          6. Must be willing and able to communicate and participate in the whole study.

          7. Must provide written informed consent.

          8. Must agree to use an adequate method of contraception.

          9. Japanese subjects enrolled in Part 4 must be first generation: born in Japan, not
             having lived outside Japan for 5 to 10 years, able to trace maternal and paternal
             Japanese ancestry, with no significant change in lifestyle, including diet (at least
             one Japanese meal consumed per day), since leaving Japan.

        Exclusion Criteria:

          1. Participation in a clinical research study within the previous 3 months.

          2. Subjects who are study site employees, or immediate family members of a study site or
             sponsor employee.

          3. Subjects who have previously been enrolled in this study.

          4. History of any drug or alcohol abuse in the past 2 years.

          5. Regular alcohol consumption in males >21 units per week and females >14 units per week
             (1 unit = ½ pint beer, 25 mL of 40% spirit or a 125 mL glass of wine).

          6. Current smokers and those who have smoked within the last 12 months. A breath carbon
             monoxide reading of greater than 10 ppm at screening.

          7. Any females of childbearing potential.

          8. Subjects who do not have suitable veins for multiple venepunctures/cannulation as
             assessed by the investigator at screening.

          9. Subjects who, after 10 min of supine rest, have a systolic blood pressure (BP) ≥140
             mmHg (≥150 mmHg in subjects >45 years of age) or a diastolic BP of ≥90 mmHg.

         10. Male subjects with QTcF interval (Fridericia's correction factor) ECG >450 msec, or
             female subjects with QTcF interval ECG >470 msec on screening or Day 1 (pre dose) ECG.

         11. Subjects with a history of serious mental illness, that includes, but is not limited
             to schizophrenia, bipolar disorder, and major depression.

         12. Subjects with glucose-6-phosphate-dehydrogenase (G6PD) deficiency.

         13. Subjects with a history of any primary malignancy, including a history of melanoma or
             suspicious undiagnosed skin lesions.

         14. Subjects with any other medical or psychological condition, deemed by the investigator
             to be likely to interfere with a subject's ability to sign informed consent,
             cooperate, or participate in the study.

         15. Subjects who have undergone major surgery within 6 months prior to screening.

         16. Clinically significant abnormal biochemistry, hematology or urinalysis as judged by
             the investigator.

         17. Positive drugs of abuse test result at screening or admission.

         18. Positive hepatitis B surface antigen (HBsAg), hepatitis C virus antibody (HCV Ab) or
             human immunodeficiency virus (HIV) results.

         19. Subjects who are taking, or have taken, any prescribed or over-the-counter drug.

         20. Subjects who have used St. John's Wort within 28 days before the first dose of study
             drug.

         21. History of cardiovascular, renal, hepatic, chronic respiratory or GI disease, or
             hematologic, lymphatic, neurolologic, endocrine, psychiatric, musculoskeletal,
             genitourinary, immunologic, dermatologic or connective tissue disease or disorders, as
             judged by the investigator.

         22. Serious adverse reaction or serious hypersensitivity to any drug or the formulation
             excipients, including history of allergy to sulfonamides.

         23. Presence or history of clinically significant allergy requiring treatment, as judged
             by the investigator.

         24. Donation or loss of greater than 400 mL of blood within the previous 3 months.

         25. Failure to satisfy the investigator of fitness to participate for any other reason.
      